GR394608

EST Overview


Name	GR394608
Unique Name	GR394608
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	745

Libraries


Library Name	Type
LIBEST_024779 ICC1882 Slow drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_SOLLC (Guanine nucleotide-binding protein alpha-1 subunit OS=Solanum lycopersicum GN=GPA1 PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.958e-17
Identity = 38/86 (44.19%), Postives = 57/86 (66.28%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + FE ++  P FE   F+L LNKFD+FE+K+ +VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVTAVIFCAAISEYDQTLFEDERKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEQKVPKVPLNACEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA2_SOYBN (Guanine nucleotide-binding protein alpha-2 subunit OS=Glycine max GN=GPA2 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.170e-17
Identity = 40/86 (46.51%), Postives = 57/86 (66.28%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + FE I+  P FE   F+L LNKFD+FE+KI +VPL  C+WF D+
Sbjct:  227 ERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENRNRMMETKELFEWILKQPCFEKTSFMLFLNKFDIFEKKILKVPLNVCEWFKDY 312

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_SOLTU (Guanine nucleotide-binding protein alpha-1 subunit OS=Solanum tuberosum GN=GPA1 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.752e-17
Identity = 38/86 (44.19%), Postives = 57/86 (66.28%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + FE ++  P FE   F+L LNKFD+FE+K+ +VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVTAVIFCAAISEYDQTLFEDERKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEQKVLKVPLNTCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_LOTJA (Guanine nucleotide-binding protein alpha-1 subunit OS=Lotus japonicus GN=GPA1 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.752e-17
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + FE ++  P FE   F+L LNKFD+FE+KI +VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEKKILKVPLNVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_LUPLU (Guanine nucleotide-binding protein alpha-1 subunit OS=Lupinus luteus GN=GPA1 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.818e-17
Identity = 40/86 (46.51%), Postives = 56/86 (65.12%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M  + + FE I+  P FE   F+L LNKFD+FE+KI +VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRMTETKELFEWILKQPCFEKTSFMLFLNKFDIFEKKILKVPLNVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA2_PEA (Guanine nucleotide-binding protein alpha-2 subunit OS=Pisum sativum GN=GPA2 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.964e-16
Identity = 39/86 (45.35%), Postives = 56/86 (65.12%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+++ + + FE I+  P FE   F+L LNKFD+FE+KI  VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVTAVIFCAAISEYDQTLFEDESKNRLMETKELFEWILKQPCFEKTSFMLFLNKFDIFEKKILNVPLNVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_ORYSJ (Guanine nucleotide-binding protein alpha-1 subunit OS=Oryza sativa subsp. japonica GN=GPA1 PE=2 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 1.964e-16
Identity = 37/86 (43.02%), Postives = 58/86 (67.44%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + F+ ++    FE   F+L LNKFD+FE+KI++VPL+ C+WF D+
Sbjct:  226 ERRKWIHLFEGVNAVIFCAAISEYDQMLFEDETKNRMMETKELFDWVLKQRCFEKTSFILFLNKFDIFEKKIQKVPLSVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_ORYSI (Guanine nucleotide-binding protein alpha-1 subunit OS=Oryza sativa subsp. indica GN=GPA1 PE=2 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 1.964e-16
Identity = 37/86 (43.02%), Postives = 58/86 (67.44%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + F+ ++    FE   F+L LNKFD+FE+KI++VPL+ C+WF D+
Sbjct:  226 ERRKWIHLFEGVNAVIFCAAISEYDQMLFEDETKNRMMETKELFDWVLKQRCFEKTSFILFLNKFDIFEKKIQKVPLSVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_PEA (Guanine nucleotide-binding protein alpha-1 subunit OS=Pisum sativum GN=GPA1 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.566e-16
Identity = 39/86 (45.35%), Postives = 56/86 (65.12%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFCV++S+YDQ   +    N+M+ + + FE ++    FE   F+L LNKFD+FE+KI  VPL  C+WF D+
Sbjct:  226 ERRKWIHLFEGVSAVIFCVAISEYDQTLFEDENKNRMMETKELFEWVLKQQCFEKTSFMLFLNKFDIFEKKILDVPLNVCEWFKDY 311

[Close]

BLAST of GR394608 vs. SwissProt
Match: GPA1_ARATH (Guanine nucleotide-binding protein alpha-1 subunit OS=Arabidopsis thaliana GN=GPA1 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.376e-16
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + F+ ++  P FE   F+L LNKFD+FE+K+  VPL  C+WF D+
Sbjct:  225 ERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFDWVLKQPCFEKTSFMLFLNKFDIFEKKVLDVPLNVCEWFRDY 310

[Close]

BLAST of GR394608 vs. TrEMBL
Match: B9HEG0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_764631 PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 5.917e-72
Identity = 138/192 (71.88%), Postives = 154/192 (80.21%), Query Frame = 3

Query:   12 VHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXX--LGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            VHARG GENCKWLEMF+DV MVIFCV+++DYDQF+VDGN      M+LS KFFE+IVTHPTFE M+FLLILNKFDLFEEKIE+VPLT+CDWF DFH                      LG L +HY+AVKFKRLYSSLTGRKLY SVVKGLEP SVDA+LKYAKEILKW+EEKPNF+ SEYSMYSTEASS+S
Sbjct:  709 VHARGLGENCKWLEMFDDVGMVIFCVAMTDYDQFTVDGNGTSTNNMMLSRKFFESIVTHPTFEQMDFLLILNKFDLFEEKIERVPLTQCDWFDDFHPVISRHRSNSNSNSNSINTSPSLGQLGAHYMAVKFKRLYSSLTGRKLYTSVVKGLEPDSVDAALKYAKEILKWDEEKPNFSLSEYSMYSTEASSYS 900

[Close]

BLAST of GR394608 vs. TrEMBL
Match: B9H6B4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831419 PE=4 SV=1)

HSP 1 Score: 270.781 bits (691), Expect = 8.544e-71
Identity = 133/192 (69.27%), Postives = 155/192 (80.73%), Query Frame = 3

Query:   12 VHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXX--LGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            VH+RG GENCKWLEMFEDV MVIFCV+++DYDQ++VDGN     KM+LS KFFE+IVTHPTFE M+FLLILNKFDLFEEKIE+VPLT+C+WF DFH                      LGHL +HY+AVKFKRLY+ LTGRKLY SVVKGLEP SVDA+LKYA+EI+KW+EEKPNF+ SEYS+YSTEASS+S
Sbjct:  694 VHSRGLGENCKWLEMFEDVGMVIFCVAMNDYDQYTVDGNGLSTNKMLLSRKFFESIVTHPTFEQMDFLLILNKFDLFEEKIERVPLTQCEWFDDFHPVISSHRSNSNSNSNSINTSPSLGHLGAHYMAVKFKRLYALLTGRKLYASVVKGLEPDSVDAALKYAREIMKWDEEKPNFSLSEYSLYSTEASSYS 885

[Close]

BLAST of GR394608 vs. TrEMBL
Match: B9S8Q3_RICCO (GTP-binding protein alpha subunit, gna, putative OS=Ricinus communis GN=RCOM_0603610 PE=4 SV=1)

HSP 1 Score: 265.388 bits (677), Expect = 3.590e-69
Identity = 130/190 (68.42%), Postives = 151/190 (79.47%), Query Frame = 3

Query:   12 VHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            VHARG GENCKWLEMFEDV MVIFCV+LSDYDQ+++DGN     KM+LS +FFE+IVTHPTF+ M+FLLILNKFDLFEEK+E+V LT C+WF DFH                    LG L  HYIAVKFK+LY+SLTG+KLYVS+VKGLEP SVDASLKYA+EILKW+EE+ NF+ SEYS YSTEASS+S
Sbjct:  727 VHARGFGENCKWLEMFEDVGMVIFCVALSDYDQYAIDGNGCSTNKMLLSRRFFESIVTHPTFDQMDFLLILNKFDLFEEKVERVSLTHCEWFDDFHPVVSHHRSNSNSNSINSSPSLGQLGFHYIAVKFKKLYASLTGKKLYVSMVKGLEPDSVDASLKYAREILKWDEERHNFSLSEYSFYSTEASSYS 916

[Close]

BLAST of GR394608 vs. TrEMBL
Match: D7UE13_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_255.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005090001 PE=4 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 3.715e-66
Identity = 130/192 (67.71%), Postives = 148/192 (77.08%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV ARG GENCKWLEMFEDV +VIFCVSL+DYDQ+S D N     KM+LS + FE+IVTHPTFE M+FLLILNKFDLFEEKIE+VPLT+CDWF DFH                    LG LA HYIAV+FK LYSSLTGRKLYVS+VKGLE  SVD +LKYA+EILKW+EE+ NF+ S+ S+YSTE SSFSH
Sbjct:  565 RVQARGLGENCKWLEMFEDVRIVIFCVSLNDYDQYSYDANGSLVNKMMLSQRLFESIVTHPTFEQMDFLLILNKFDLFEEKIERVPLTQCDWFEDFHPVVSRNRSNSNSNNINNSPSLGQLAFHYIAVRFKTLYSSLTGRKLYVSLVKGLELNSVDETLKYAREILKWDEERANFSLSD-SVYSTEPSSFSH 755

[Close]

BLAST of GR394608 vs. TrEMBL
Match: A5AKX9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002365 PE=4 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 3.715e-66
Identity = 130/192 (67.71%), Postives = 148/192 (77.08%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV ARG GENCKWLEMFEDV +VIFCVSL+DYDQ+S D N     KM+LS + FE+IVTHPTFE M+FLLILNKFDLFEEKIE+VPLT+CDWF DFH                    LG LA HYIAV+FK LYSSLTGRKLYVS+VKGLE  SVD +LKYA+EILKW+EE+ NF+ S+ S+YSTE SSFSH
Sbjct:  866 RVQARGLGENCKWLEMFEDVRIVIFCVSLNDYDQYSYDANGSLVNKMMLSQRLFESIVTHPTFEQMDFLLILNKFDLFEEKIERVPLTQCDWFEDFHPVVSRNRSNSNSNNINNSPSLGQLAFHYIAVRFKTLYSSLTGRKLYVSLVKGLELNSVDETLKYAREILKWDEERANFSLSD-SVYSTEPSSFSH 1056

[Close]

BLAST of GR394608 vs. TrEMBL
Match: D7LFC2_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_667596 PE=4 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 2.183e-58
Identity = 116/188 (61.70%), Postives = 140/188 (74.47%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG-NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV +RG GENCKW++MFEDV MV+F VS+SDYDQ S DG NKM+L+ K FE+I+THP FEHM+FLLILNK+DL EEK+E+VPL+ C WF DF+                    LG LA H++AVKFKR YSSLTG+KL+VS  K L+P SVD+SLK A EILKW+EE+ N   SEYSMYSTE SSFS+
Sbjct:  709 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDGTNKMLLTKKLFESIITHPIFEHMDFLLILNKYDLLEEKVERVPLSHCKWFQDFNPVVSHHRGSNNGNPT-----LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICLSEYSMYSTEPSSFSN 891

[Close]

BLAST of GR394608 vs. TrEMBL
Match: O81225_ARATH (Extra-large G-protein OS=Arabidopsis thaliana GN=XLG PE=4 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 2.851e-58
Identity = 115/188 (61.17%), Postives = 141/188 (75.00%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG-NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV +RG GENCKW++MFEDV MV+F VS+SDYDQ S DG NKM+L+ K FE+I+THP FE+M+FLLILNK+DL EEK+E+VPL +C+WF DF+                    LG LA H++AVKFKR YSSLTG+KL+VS  K L+P SVD+SLK A EILKW+EE+ N   SEYSMYSTE SSFS+
Sbjct:  706 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDGTNKMLLTKKLFESIITHPIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHRGSNNGNPT-----LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888

[Close]

BLAST of GR394608 vs. TrEMBL
Match: O80462_ARATH (Extra-large G-protein OS=Arabidopsis thaliana GN=XLG PE=2 SV=2)

HSP 1 Score: 229.18 bits (583), Expect = 2.851e-58
Identity = 115/188 (61.17%), Postives = 141/188 (75.00%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG-NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV +RG GENCKW++MFEDV MV+F VS+SDYDQ S DG NKM+L+ K FE+I+THP FE+M+FLLILNK+DL EEK+E+VPL +C+WF DF+                    LG LA H++AVKFKR YSSLTG+KL+VS  K L+P SVD+SLK A EILKW+EE+ N   SEYSMYSTE SSFS+
Sbjct:  706 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDGTNKMLLTKKLFESIITHPIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHRGSNNGNPT-----LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888

[Close]

BLAST of GR394608 vs. TrEMBL
Match: B9HRU3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_876286 PE=4 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 2.338e-52
Identity = 110/191 (57.59%), Postives = 132/191 (69.11%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            RVH    G NCKWLEMFEDV++V+FCVSL DYD+FS D N     KMI S + FE I THPTFE  +FLLILNKFDL EEKIEQVPLT+C+WF DF+                    L   A  YIAVKFKRL++SLT RKLYVS   GLEP +VD +LKYA+E+L+W +E+PN++++E S  S EASS S
Sbjct:  669 RVHPTTLGGNCKWLEMFEDVDIVLFCVSLIDYDEFSEDNNGVPINKMIASRQLFERIATHPTFEEKKFLLILNKFDLLEEKIEQVPLTRCEWFHDFNPVMGNNPNSSRSTNSP----LELRAFQYIAVKFKRLFNSLTDRKLYVSKCTGLEPDNVDEALKYAREVLRWEQEEPNYHNNELSSTSIEASSSS 855

[Close]

BLAST of GR394608 vs. TrEMBL
Match: B9N4S6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_267688 PE=4 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 5.389e-49
Identity = 106/191 (55.50%), Postives = 127/191 (66.49%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDGN-----KMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            RVH    G NCK LEMFEDV++V+FCVSL DYD+FS D N     KMI S + FE   THPTFE  +FLLILNKFDL EEKI+QVPLT+C+WF DF+                    L   AS YIAVKFKRL+  LT RKLYVS+  GLEP +VD + KYA+E+LKW +E+ N+ ++E S  S EASS S
Sbjct:  519 RVHPTTLGGNCKRLEMFEDVDVVLFCVSLIDYDEFSEDKNGVLINKMIASRQLFERTATHPTFEEKKFLLILNKFDLLEEKIQQVPLTQCEWFDDFNPVIGYNPNSSSSTNPS----LARRASQYIAVKFKRLFRDLTDRKLYVSLATGLEPDNVDEAFKYAREVLKWKQEELNYPNNELSSTSIEASSSS 705

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT2G23460.1 (| Symbols: XLG1, ATXLG1 | extra-large G-protein 1 | chr2:9995699-9998945 FORWARD LENGTH=888)

HSP 1 Score: 229.18 bits (583), Expect = 1.866e-60
Identity = 115/188 (61.17%), Postives = 141/188 (75.00%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG-NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFSH 569
            RV +RG GENCKW++MFEDV MV+F VS+SDYDQ S DG NKM+L+ K FE+I+THP FE+M+FLLILNK+DL EEK+E+VPL +C+WF DF+                    LG LA H++AVKFKR YSSLTG+KL+VS  K L+P SVD+SLK A EILKW+EE+ N   SEYSMYSTE SSFS+
Sbjct:  706 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDGTNKMLLTKKLFESIITHPIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHRGSNNGNPT-----LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT1G31930.3 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 168.703 bits (426), Expect = 2.994e-42
Identity = 89/196 (45.41%), Postives = 118/196 (60.20%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---------NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNF---NSSEYSMYSTEASS 560
            RV+A+G  ++CKW+EMFEDV  VIFC+SLSDYDQ ++           NKMI S + FE++V HP F+   F+LILNK+D FEEK+ + PLT CDWFSDF                     L + A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW+EEK         E S YST+ SS
Sbjct:  656 RVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSCDWFSDF----------CPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT1G31930.2 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 168.703 bits (426), Expect = 2.994e-42
Identity = 89/196 (45.41%), Postives = 118/196 (60.20%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---------NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNF---NSSEYSMYSTEASS 560
            RV+A+G  ++CKW+EMFEDV  VIFC+SLSDYDQ ++           NKMI S + FE++V HP F+   F+LILNK+D FEEK+ + PLT CDWFSDF                     L + A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW+EEK         E S YST+ SS
Sbjct:  656 RVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSCDWFSDF----------CPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT1G31930.1 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 168.703 bits (426), Expect = 2.994e-42
Identity = 89/196 (45.41%), Postives = 118/196 (60.20%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---------NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSSLTGRKLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNF---NSSEYSMYSTEASS 560
            RV+A+G  ++CKW+EMFEDV  VIFC+SLSDYDQ ++           NKMI S + FE++V HP F+   F+LILNK+D FEEK+ + PLT CDWFSDF                     L + A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW+EEK         E S YST+ SS
Sbjct:  656 RVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSCDWFSDF----------CPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT4G34390.1 (| Symbols: XLG2 | extra-large GTP-binding protein 2 | chr4:16441579-16444840 FORWARD LENGTH=861)

HSP 1 Score: 146.362 bits (368), Expect = 1.591e-35
Identity = 91/201 (45.27%), Postives = 117/201 (58.21%), Query Frame = 3

Query:    9 RVHARGPGENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG-----NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDFHXXXXXXXXXXXXXXXXXXXXLGHLASHYIAVKFKRLYSS------LTGR----KLYVSVVKGLEPGSVDASLKYAKEILKWNEEKPNFNSSEYSMYSTEASSFS 566
            R++ R  GEN K LEMFED ++VIFCVSL+DY +   DG     NKM+ + + FE +VTHP+  +  FLL+L KFDL EEKIE+VPL  C+WF DF+                    +   A HYI  KFKRLY S      + GR    KL+V  V  LE  +VD +L+YA+EILKW+ E+ +    E S  S EASS S
Sbjct:  669 RLNPRSLGENWKLLEMFEDADLVIFCVSLTDYAENIEDGEGNIVNKMLATKQLFENMVTHPSLANKRFLLVLTKFDLLEEKIEEVPLRTCEWFEDFN------PLISQNQTSRHNPPMAQRAFHYIGYKFKRLYDSILEPVNMRGRSFKPKLFVCQV-SLESDTVDNALRYAREILKWHVEETSM-FQEMSTTSIEASSSS 861

[Close]

BLAST of GR394608 vs. TAIR peptide
Match: AT2G26300.1 (| Symbols: GPA1, GP ALPHA 1, ATGPA1 | G protein alpha subunit 1 | chr2:11198087-11200822 FORWARD LENGTH=383)

HSP 1 Score: 85.1149 bits (209), Expect = 4.353e-17
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 3

Query:   33 ENCKWLEMFEDVEMVIFCVSLSDYDQFSVDG---NKMILSMKFFETIVTHPTFEHMEFLLILNKFDLFEEKIEQVPLTKCDWFSDF 281
            E  KW+ +FE V  VIFC ++S+YDQ   +    N+M+ + + F+ ++  P FE   F+L LNKFD+FE+K+  VPL  C+WF D+
Sbjct:  225 ERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFDWVLKQPCFEKTSFMLFLNKFDIFEKKVLDVPLNVCEWFRDY 310

The following BLAST results are available for this feature:

BLAST of GR394608 vs. SwissProt
BLAST of GR394608 vs. TrEMBL
BLAST of GR394608 vs. TAIR peptide

BLAST of GR394608 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GPA1_SOLLC	3.958e-17	44.19	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA2_SOYBN	5.170e-17	46.51	Guanine nucleotide-binding protein alpha-2 subunit...	[more]
GPA1_SOLTU	6.752e-17	44.19	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA1_LOTJA	6.752e-17	45.35	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA1_LUPLU	8.818e-17	46.51	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA2_PEA	1.964e-16	45.35	Guanine nucleotide-binding protein alpha-2 subunit...	[more]
GPA1_ORYSJ	1.964e-16	43.02	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA1_ORYSI	1.964e-16	43.02	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA1_PEA	2.566e-16	45.35	Guanine nucleotide-binding protein alpha-1 subunit...	[more]
GPA1_ARATH	4.376e-16	43.02	Guanine nucleotide-binding protein alpha-1 subunit...	[more]

BLAST of GR394608 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HEG0_POPTR	5.917e-72	71.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H6B4_POPTR	8.544e-71	69.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S8Q3_RICCO	3.590e-69	68.42	GTP-binding protein alpha subunit, gna, putative O...	[more]
D7UE13_VITVI	3.715e-66	67.71	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AKX9_VITVI	3.715e-66	67.71	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7LFC2_ARALY	2.183e-58	61.70	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
O81225_ARATH	2.851e-58	61.17	Extra-large G-protein OS=Arabidopsis thaliana GN=X...	[more]
O80462_ARATH	2.851e-58	61.17	Extra-large G-protein OS=Arabidopsis thaliana GN=X...	[more]
B9HRU3_POPTR	2.338e-52	57.59	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N4S6_POPTR	5.389e-49	55.50	Predicted protein (Fragment) OS=Populus trichocarp...	[more]

BLAST of GR394608 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT2G23460.1	1.866e-60	61.17	\| Symbols: XLG1, ATXLG1 \| extra-large G-protein 1 ...	[more]
AT1G31930.3	2.994e-42	45.41	\| Symbols: XLG3 \| extra-large GTP-binding protein ...	[more]
AT1G31930.2	2.994e-42	45.41	\| Symbols: XLG3 \| extra-large GTP-binding protein ...	[more]
AT1G31930.1	2.994e-42	45.41	\| Symbols: XLG3 \| extra-large GTP-binding protein ...	[more]
AT4G34390.1	1.591e-35	45.27	\| Symbols: XLG2 \| extra-large GTP-binding protein ...	[more]
AT2G26300.1	4.353e-17	43.02	\| Symbols: GPA1, GP ALPHA 1, ATGPA1 \| G protein al...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	PANTHER	PTHR10218	GTP-BINDING PROTEIN ALPHA SUBUNIT	coord: 9..140 score: 3.9
IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	PFAM	PF00503	G-alpha	coord: 12..163 score: 1.8
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 11..169 score: 5.4
None	No IPR available	PANTHER	PTHR10218:SF90	EXTRA-LARGE G-PROTEIN	coord: 9..140 score: 3.9
None	No IPR available	SEG	seg	seg	coord: 95..114 score
None	No IPR available	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 14..169 score: 3.7
None	No IPR available	SEG	seg	seg	coord: 18..33 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR394608 ID=GR394608; Name=GR394608; organism=Cicer arietinum; type=EST; length=745bp
ATCAACGCAGAGTGCATGCAAGGGGGCCAGGAGAAAATTGCAAGTGGCTA
GAGATGTTTGAGGATGTTGAAATGGTCATCTTCTGTGTTTCCTTGAGTGA
CTATGATCAGTTTTCTGTGGATGGAAACAAGATGATATTGAGCATGAAGT
TTTTTGAAACAATTGTCACTCATCCAACTTTTGAACACATGGAATTCCTG
TTGATATTGAACAAATTTGATCTATTTGAGGAGAAAATTGAACAAGTTCC
TTTGACCAAATGTGATTGGTTCTCTGATTTTCATCCAATAACTAGTCGCA
ATCGCACCAACAGCAATAGCAACAGCATTAATAATAATCCCTCCCTTGGT
CACCTGGCTTCCCATTACATAGCAGTTAAGTTTAAAAGACTTTATTCATC
TCTTACAGGACGAAAGTTGTATGTTTCTGTGGTGAAAGGGTTGGAACCAG
GTAGTGTTGATGCATCGCTTAAATATGCCAAGGAAATTTTGAAGTGGAAT
GAAGAGAAACCAAATTTTAACTCAAGTGAGTACTCAATGTATAGCACTGA
GGCGAGTTCATTCTCTCATTGATTACTAGTTCAGGCATGAGTTGCAAGTC
ATACTTGTGTATGACTTGAATTATGCCTTGTACATATATTGCTAACTAGG
AGTAACAGGGAATGTACCTTCCACTAGCTATCAACTTGTAAGATGCACTA
GACACGTGTAGGTAAGATTTCATGTGGTTAGGGAATGCTGGTGAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004871	obsolete signal transducer activity
GO:0019001	guanyl nucleotide binding

Vocabulary: **Biological Process**
Term	Definition
GO:0007186	G protein-coupled receptor signaling pathway

Vocabulary: **INTERPRO**
Term	Definition
IPR001019	Gprotein_alpha_su

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