GR396736

EST Overview


Name	GR396736
Unique Name	GR396736
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	728

Libraries


Library Name	Type
LIBEST_024782 ICC4958 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR396736 vs. SwissProt
Match: CP74A_ARATH (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana GN=CYP74A PE=1 SV=3)

HSP 1 Score: 225.713 bits (574), Expect = 1.997e-58
Identity = 108/222 (48.65%), Postives = 147/222 (66.22%), Query Frame = 3

Query:   60 IPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            IPG+YGLP++GP+ DR DYFY++G ++FF SR++KYNSTV +VNMPPG FIA NP+V+ALLDG SFP+LFD  KV+K+D+  GT+MPST+  GGYR  ++ D +EP H+ +K  +F +L S      P F +  S+ F  LEK+L+ K  KA F  S     FNFL R     NP +TK+    P L+  W+   L PL + GLP++   IE+ LI T  +P
Sbjct:   57 IPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLK-GKADFGGSSDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRV---IEEPLIHTFSLP 274

[Close]

BLAST of GR396736 vs. SwissProt
Match: CP74_LINUS (Allene oxide synthase, chloroplastic OS=Linum usitatissimum GN=CYP74A PE=1 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 1.690e-57
Identity = 107/224 (47.77%), Postives = 150/224 (66.96%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            + IPG YGLP IGP+ DR DYFYN+GR++FF SR+QKY STV + NMPPG FIA NP+VI LLD  SFP+LFD SKV+K+D+  GT+MPST+  GGYR  ++ D +EP+H  +K  +F+++ ++ +  IP FSS+ +D    +E DLA K  KA+FN     A FNFL R      P +T +G   P+L+  W+   LAP+ + GLPK    +E+  + ++ +P
Sbjct:   73 RQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGPFIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATK-GKAAFNDPAEQAAFNFLSRAFFGVKPIDTPLGKDAPSLISKWVLFNLAPILSVGLPK---EVEEATLHSVRLP 292

[Close]

BLAST of GR396736 vs. SwissProt
Match: C74A2_ORYSJ (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 213.772 bits (543), Expect = 7.852e-55
Identity = 102/225 (45.33%), Postives = 145/225 (64.44%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPT-ETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            +P+PGSYG+P +  + DR D++Y +G+DK+F SR ++Y STV+++N+PPG F+A +P+V+ALLD  SFP+LFD +KV+KRDV  GTFMPST   GGYR CA+ D +EP+H  IK  +  +L S+ + F+P+F SN       +E  LA    K+ F +     +F F+        P+  + +G  GPT    WL  QLAPL T GLP I   IED L+ T+P+P
Sbjct:   10 RPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDPRVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLLLSLLVSRKDAFVPVFRSNFGALLDTVESQLASGGGKSDFTALNDATSFEFIGEAYFGVRPSASSSLGTGGPTKAALWLLWQLAPLTTLGLPMI---IEDPLLHTLPLP 231

[Close]

BLAST of GR396736 vs. SwissProt
Match: C74A2_PARAR (Allene oxide synthase OS=Parthenium argentatum GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 213.386 bits (542), Expect = 1.025e-54
Identity = 108/226 (47.79%), Postives = 145/226 (64.16%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEG-RDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            + IPGSYG+P   P+ DR +YFY  G RD++F SR+QKY STV + NMPPG F++ NPKVI LLD  SFPILFD SKV+K+D+  GT+MPST   GGYR  ++ D +EP H  +K  +F +L +     IP F +  ++ F  LE +LA K+ KA+FN     A F FL R   N NP ETK+G S PTL+ +W+   LAP    GLP    ++++ L+ T  +PA
Sbjct:    9 REIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPFVSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELA-KNGKAAFNDVGEQAAFRFLGRAYFNSNPEETKLGTSAPTLISSWVLFNLAPTLDLGLPW---FLQEPLLHTFRLPA 230

[Close]

BLAST of GR396736 vs. SwissProt
Match: C74A1_ORYSJ (Allene oxide synthase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=CYP74A1 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 4.308e-53
Identity = 102/225 (45.33%), Postives = 148/225 (65.78%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEG-RDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            + +PG YG PV+G + DR++YFY  G RD FFA+RV+ + STV+++NMPPG F+A +P+V+ALLD  SFP+LFD S VDK D+  GTFMPSTD  GGYR  ++ D +EP+H  +KT +F++LS + +  IP F     D F  +E DLA +  KA F      A F FL + L  ++P ++ +G  GP L+  W+  QL+PL + GLP +   +ED L+ ++ +P
Sbjct:   48 RKVPGDYGPPVVGAIRDRYEYFYGPGGRDGFFAARVRAHRSTVVRLNMPPGPFVARDPRVVALLDAASFPVLFDTSLVDKTDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHAPLKTLLFYLLSHRRQQVIPKFREVYGDLFGLMENDLA-RVGKADFGVHNDAAAFGFLCQGLLGRDPAKSALGRDGPKLITKWVLFQLSPLLSLGLPTL---VEDTLLHSLRLP 268

[Close]

BLAST of GR396736 vs. SwissProt
Match: C74A3_ORYSJ (Allene oxide synthase 3 OS=Oryza sativa subsp. japonica GN=CYP74A3 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.589e-39
Identity = 89/225 (39.56%), Postives = 126/225 (56.00%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYN-STVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            + IPG YG+P   PL DR DYFY +G +++F SRV ++  +TV++VNMPPG FI+ NP+V+ALLD  SF +L D+S VDK D LDGT+MPS   FGG+R  AF D  +P H  IK  +  + +++     P F +  +  F  +E   AG  A   FN        +F    L    P    +GD   T    WLA QL P+A+  +      +E++L+ T  +P
Sbjct:   30 REIPGGYGVPFFSPLRDRLDYFYFQGAEEYFRSRVARHGGATVLRVNMPPGPFISGNPRVVALLDARSFRVLLDDSMVDKADTLDGTYMPSRALFGGHRPLAFLDAADPRHAKIKRVVMSLAAARMHHVAPAFRAAFAAMFDAVE---AGLGAAVEFNKLNMRYMLDFTCAALFGGEPPSKVVGDGAVTKAMAWLAFQLHPIASKVVKPW--PLEELLLHTFSLP 249

[Close]

BLAST of GR396736 vs. SwissProt
Match: C74A4_ORYSJ (Allene oxide synthase 4 OS=Oryza sativa subsp. japonica GN=CYP74A4 PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.030e-38
Identity = 88/225 (39.11%), Postives = 126/225 (56.00%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYN-STVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            + IPG YG+P + PL DR DY+Y +G D+FF SRV ++  +TV++VNMPPG F+A +P+V+ALLD  SF +L D+S VDK D LDGTFMPS   FGG+R  AF D  +P H  IK  +  + +++     P F +  +  F +++  L        FN        +F    L    P    +GD+  T    WL  QL PLA+  +      +ED+L+ T  +P
Sbjct:   30 REIPGGYGVPFLSPLRDRLDYYYFQGADEFFRSRVARHGGATVLRVNMPPGPFLAGDPRVVALLDARSFRVLLDDSMVDKADTLDGTFMPSLALFGGHRPLAFLDAADPRHAKIKRVVMSLAAARMHHVAPAFRAAFAAMFDEVDAGLVA-GGPVEFNKLNMRYMLDFTCAALFGGAPPSKAMGDAAVTKAVKWLIFQLHPLASKVVKPW--PLEDLLLHTFRLP 251

[Close]

BLAST of GR396736 vs. TrEMBL
Match: Q7X9B3_MEDTR (9/13 hydroperoxide lyase OS=Medicago truncatula GN=hpl1 PE=2 SV=1)

HSP 1 Score: 416.001 bits (1068), Expect = 1.572e-114
Identity = 190/225 (84.44%), Postives = 216/225 (96.00%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            KPIPGSYGLP+IGPLHDRHDYFYN+GRDK+F +R++KYNSTV+K+NMPPGGFIAP+PKVIALLDG SFPILFDN+KV+KRDVLDGTFMPSTD+FGGYRTCAFQDT EPSH L+K FIFHILSSKH+TFIPLF +N+++HFTDLEK+LAGKH KASFN+SIG  TFNFLF+L+T+KNP+ETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNY+ED+LIRTIPIPA
Sbjct:   15 KPIPGSYGLPIIGPLHDRHDYFYNQGRDKYFQTRIEKYNSTVLKLNMPPGGFIAPDPKVIALLDGASFPILFDNAKVEKRDVLDGTFMPSTDFFGGYRTCAFQDTAEPSHSLLKRFIFHILSSKHDTFIPLFQTNLTEHFTDLEKELAGKHQKASFNTSIGGITFNFLFKLITDKNPSETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYLEDVLIRTIPIPA 239

[Close]

BLAST of GR396736 vs. TrEMBL
Match: B7FLW4_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 409.838 bits (1052), Expect = 1.127e-112
Identity = 188/225 (83.56%), Postives = 214/225 (95.11%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            KPIPGSYGLP+IGPLHDRHDYFYN+GRDK+F +R++KYNSTV+K+NMPPGGFIA +PKVIALLDG SFPILFDN+KV+KRDVLDGTFMPSTD+FGGYRTCAFQDT EPSH L+K FIFHILSSKH+TFIPL  +N+++HFTDLEK+LAGKH KASFN+SIG  TFNFLF+L+T+KNP+ETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNY+ED+LIRTIPIPA
Sbjct:   15 KPIPGSYGLPIIGPLHDRHDYFYNQGRDKYFQTRIEKYNSTVLKLNMPPGGFIASDPKVIALLDGASFPILFDNAKVEKRDVLDGTFMPSTDFFGGYRTCAFQDTAEPSHSLLKRFIFHILSSKHDTFIPLSQTNLTEHFTDLEKELAGKHQKASFNTSIGGITFNFLFKLITDKNPSETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYLEDVLIRTIPIPA 239

[Close]

BLAST of GR396736 vs. TrEMBL
Match: Q7X9B2_MEDTR (9/13 hydroperoxide lyase OS=Medicago truncatula GN=hpl2 PE=2 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 4.618e-82
Identity = 144/226 (63.72%), Postives = 177/226 (78.32%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLL-TNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            K IPGSYGLP IGP+ DRHDYFYN+GRDKFF++R+QKYNST+ + NMPPG FI+ NP+VIALLD  SFPILFDN KV+K +VLDGTFMPST + GGYR CA+ DTTEP+H LIK F  + L  + +TFIPLF + +SD F ++E  L+ K  KA FNS +  A+FNF+F+L   +KNP+ET +GD GP +  TWL  QLAPLAT G PKIFNY+EDIL+RT+P PA
Sbjct:   18 KQIPGSYGLPFIGPIFDRHDYFYNQGRDKFFSTRIQKYNSTIFRTNMPPGPFISSNPRVIALLDAASFPILFDNKKVEKLNVLDGTFMPSTKFTGGYRVCAYLDTTEPNHALIKGFYLNTLLLRKDTFIPLFKTILSDGFNEIEDGLSSKSGKADFNSMVSVASFNFMFKLFCDDKNPSETILGDQGPKMFDTWLLFQLAPLATLGPPKIFNYLEDILLRTVPFPA 243

[Close]

BLAST of GR396736 vs. TrEMBL
Match: Q2LAJ5_SOYBN (Cytochrome P450 monooxygenase CYP74A1 OS=Glycine max GN=CYP74A1 PE=2 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 1.065e-78
Identity = 136/226 (60.18%), Postives = 180/226 (79.65%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTN-KNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            KPIPGSYG+P  G + DRH+YFY++GRDKFFA+R++K+NSTVI+ NMPPG FI+ +P+V+ALLDG SFPILFDN KV+K +VLDGTFMPST + GG+R CA+ DTTEP+H LIK F  ++L+ + ++F+PLF + + + F ++E  L+ K+ KA FN+   +A+FNF+FRL  + K+P++T +G  GP LV TWL  QLAPLAT GLPKIFNYIED LIRT+P PA
Sbjct:   21 KPIPGSYGMPFFGAISDRHNYFYHQGRDKFFATRIEKHNSTVIRTNMPPGPFISSDPRVVALLDGASFPILFDNDKVEKLNVLDGTFMPSTKFTGGFRVCAYLDTTEPNHALIKQFFLNVLAKRKDSFVPLFRNCLQESFAEIEDQLS-KNTKADFNTVFSDASFNFMFRLFCDGKDPSQTNLGSKGPKLVDTWLLFQLAPLATLGLPKIFNYIEDFLIRTLPFPA 245

[Close]

BLAST of GR396736 vs. TrEMBL
Match: B9P6E9_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP74C6v1-2 PE=3 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 1.140e-72
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            KPIPGSYGLP  G + DR DYFYN+G+D+FF+SRV+KY STV K NMPPG FIA NPKVIA+LD +SFPILFD SK++K +VLDGT++PS  + GGYR CA+ D +EP+H  +K+F   +L+SKH+ F+PLF + +S  F D+E ++  K   A+FN S    +FNF+FRL   K+P+ETK+G  GP +V  W+  QLAPLAT G PK   + ED+L+ T PIP
Sbjct:   11 KPIPGSYGLPFFGAIKDRLDYFYNQGKDEFFSSRVEKYQSTVFKTNMPPGPFIAQNPKVIAVLDAISFPILFDTSKIEKFNVLDGTYLPSLSFTGGYRVCAYLDPSEPNHTSLKSFFMSVLASKHKDFVPLFRTCLSQMFIDIEDEMGSKRT-ANFNDSSDAMSFNFVFRLFCEKDPSETKLGSEGPAIVDKWVGLQLAPLATIGFPKFLKHFEDLLMHTFPIP 233

[Close]

BLAST of GR396736 vs. TrEMBL
Match: B9H0K5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_759609 PE=3 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 1.140e-72
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            KPIPGSYGLP  G + DR DYFYN+G+D+FF+SRV+KY STV K NMPPG FIA NPKVIA+LD +SFPILFD SK++K +VLDGT++PS  + GGYR CA+ D +EP+H  +K+F   +L+SKH+ F+PLF + +S  F D+E ++  K   A+FN S    +FNF+FRL   K+P+ETK+G  GP +V  W+  QLAPLAT G PK   + ED+L+ T PIP
Sbjct:   24 KPIPGSYGLPFFGAIKDRLDYFYNQGKDEFFSSRVEKYQSTVFKTNMPPGPFIAQNPKVIAVLDAISFPILFDTSKIEKFNVLDGTYLPSLSFTGGYRVCAYLDPSEPNHTSLKSFFMSVLASKHKDFVPLFRTCLSQMFIDIEDEMGSKRT-ANFNDSSDAMSFNFVFRLFCEKDPSETKLGSEGPAIVDKWVGLQLAPLATIGFPKFLKHFEDLLMHTFPIP 246

[Close]

BLAST of GR396736 vs. TrEMBL
Match: B9HRU0_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP74C8 PE=3 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 1.260e-71
Identity = 127/224 (56.70%), Postives = 164/224 (73.21%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            K IPG YGLP  G + DR DYFYN+GRD+FF++RVQKY ST+ K NMPPG FIA NPKVIA+LD VSFP+LFD SKV+K +VLDGTF PS  + GGYR CA+ D +EP H  +K+F    L+SKH  FIPLF +++S  F ++E ++  +  KA+FN      +F+F+FRL  +K+ ++TK+G  GP +V  WLA QLAPLAT GLPK F Y+EDI +RT P+P
Sbjct:   16 KSIPGDYGLPFFGAIRDRLDYFYNQGRDEFFSTRVQKYESTIFKTNMPPGPFIAKNPKVIAVLDAVSFPVLFDTSKVEKYNVLDGTFFPSVSFTGGYRACAYLDPSEPQHTSLKSFFMSTLASKHNDFIPLFRASLSKLFINIEDEIVSQ-KKANFNKPSEAMSFDFVFRLFGDKDSSDTKVGSEGPAIVNKWLALQLAPLATLGLPKYFKYLEDIFLRTFPLP 238

[Close]

BLAST of GR396736 vs. TrEMBL
Match: B9H0K6_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP74C7-1 PE=3 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 7.647e-69
Identity = 122/224 (54.46%), Postives = 159/224 (70.98%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            KPIPG YG P  G + DR DYFYN+G+D+FF +R+QK+NSTVIK NMPPG FIA NPKVIA+LD +SFPILFD SKV+K +VLDGTF+PS  Y GGYR CA+ D +E  H  +K++   +L+SKH  FIPLF + +S  F  +E  +A +  KA+FN+     +FN++FRL   K+P+ET IG  GP +   WLA QL PL T GLPK   Y++D+L+ T  +P
Sbjct:   16 KPIPGDYGTPFFGAIRDRLDYFYNQGKDEFFKTRIQKHNSTVIKTNMPPGPFIAKNPKVIAVLDAISFPILFDTSKVEKYNVLDGTFLPSLSYSGGYRVCAYLDPSETKHTALKSYFMSVLASKHSEFIPLFRTCLSQLFISIEDGIASQ-KKANFNNVCQVMSFNYIFRLFCGKDPSETAIGSKGPAIADKWLALQLGPLFTLGLPKFLKYVDDLLLHTFKLP 238

[Close]

BLAST of GR396736 vs. TrEMBL
Match: A9PC92_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 7.647e-69
Identity = 122/224 (54.46%), Postives = 159/224 (70.98%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            KPIPG YG P  G + DR DYFYN+G+D+FF +R+QK+NSTVIK NMPPG FIA NPKVIA+LD +SFPILFD SKV+K +VLDGTF+PS  Y GGYR CA+ D +E  H  +K++   +L+SKH  FIPLF + +S  F  +E  +A +  KA+FN+     +FN++FRL   K+P+ET IG  GP +   WLA QL PL T GLPK   Y++D+L+ T  +P
Sbjct:   16 KPIPGDYGTPFFGAIRDRLDYFYNQGKDEFFKTRIQKHNSTVIKTNMPPGPFIAKNPKVIAVLDAISFPILFDTSKVEKYNVLDGTFLPSLSYSGGYRVCAYLDPSETKHTALKSYFMSVLASKHSEFIPLFRTCLSQLFISIEDGIASQ-KKANFNNVCQVMSFNYIFRLFCGKDPSETAIGSKGPAIADKWLALQLGPLFTLGLPKFLKYVDDLLLHTFKLP 238

[Close]

BLAST of GR396736 vs. TrEMBL
Match: Q7XB42_PRUDU (Cytochrome P450 OS=Prunus dulcis GN=cyp74C5 PE=3 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 3.552e-66
Identity = 124/225 (55.11%), Postives = 156/225 (69.33%), Query Frame = 3

Query:   54 KPIPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            KPIPG YG P  G + DR+DYFYN+GR  FF +R++KY STV + NMPPG  IA NPKVIALLD  SFPI+FDN+KV +RDVLDGT+MPST Y GGYR CA+ D +EP+H  +K++   +L+S+H  FIPLF S+ SD F +LE  L+ K  KA FN+   + +FNF F L   ++P+ TK+G  GP+LV  WL  QLAP  T GLPK     ED L+ T   PA
Sbjct:   17 KPIPGDYGWPFFGHIKDRYDYFYNQGRYDFFKTRIEKYQSTVFRTNMPPGILIASNPKVIALLDAKSFPIIFDNTKVLRRDVLDGTYMPSTAYTGGYRVCAYLDPSEPNHATLKSYFAALLASQHTKFIPLFQSSTSDMFLNLEAQLS-KDGKAYFNTLSDDMSFNFAFELFCGQSPSNTKLGSKGPSLVTLWLFPQLAPQITFGLPKFLALAEDFLLHTFSYPA 240

[Close]

BLAST of GR396736 vs. TAIR peptide
Match: AT5G42650.1 (| Symbols: AOS, CYP74A, DDE2 | allene oxide synthase | chr5:17097803-17099359 REVERSE LENGTH=518)

HSP 1 Score: 225.713 bits (574), Expect = 1.994e-59
Identity = 108/222 (48.65%), Postives = 147/222 (66.22%), Query Frame = 3

Query:   60 IPGSYGLPVIGPLHDRHDYFYNEGRDKFFASRVQKYNSTVIKVNMPPGGFIAPNPKVIALLDGVSFPILFDNSKVDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPTETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIP 725
            IPG+YGLP++GP+ DR DYFY++G ++FF SR++KYNSTV +VNMPPG FIA NP+V+ALLDG SFP+LFD  KV+K+D+  GT+MPST+  GGYR  ++ D +EP H+ +K  +F +L S      P F +  S+ F  LEK+L+ K  KA F  S     FNFL R     NP +TK+    P L+  W+   L PL + GLP++   IE+ LI T  +P
Sbjct:   57 IPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLK-GKADFGGSSDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRV---IEEPLIHTFSLP 274

[Close]

BLAST of GR396736 vs. TAIR peptide
Match: AT4G15440.1 (| Symbols: HPL1, CYP74B2 | hydroperoxide lyase 1 | chr4:8835869-8838462 FORWARD LENGTH=384)

HSP 1 Score: 82.8037 bits (203), Expect = 2.088e-16
Identity = 50/150 (33.33%), Postives = 79/150 (52.67%), Query Frame = 3

Query:  282 VDKRDVLDGTFMPSTDYFGGYRTCAFQDTTEPSHKLIKTFIFHILSSKHETFIPLFSSNISDHFTDLEKDLAGKHAKASFNSSIGNATFNFLFRLLTNKNPT-ETKIGDSGPTLVQTWLAAQLAPLATAGLPKIFNYIEDILIRTIPIPA 728
            VDKRDVL G F PS  ++GG R   + DTTEP H  IK F    L    + ++    SN++  +  +E +++ K+  AS+   +    F+FL   L   + +    I ++G   + TWLA Q+ P A  G+  +   +E+IL+ T P P+
Sbjct:    4 VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNLNIFWGTIESEIS-KNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLALQVIPTAKLGV--VPQPLEEILLHTWPYPS 150

The following BLAST results are available for this feature:

BLAST of GR396736 vs. SwissProt
BLAST of GR396736 vs. TrEMBL
BLAST of GR396736 vs. TAIR peptide

BLAST of GR396736 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
CP74A_ARATH	1.997e-58	48.65	Allene oxide synthase, chloroplastic OS=Arabidopsi...	[more]
CP74_LINUS	1.690e-57	47.77	Allene oxide synthase, chloroplastic OS=Linum usit...	[more]
C74A2_ORYSJ	7.852e-55	45.33	Allene oxide synthase 2 OS=Oryza sativa subsp. jap...	[more]
C74A2_PARAR	1.025e-54	47.79	Allene oxide synthase OS=Parthenium argentatum GN=...	[more]
C74A1_ORYSJ	4.308e-53	45.33	Allene oxide synthase 1, chloroplastic OS=Oryza sa...	[more]
C74A3_ORYSJ	1.589e-39	39.56	Allene oxide synthase 3 OS=Oryza sativa subsp. jap...	[more]
C74A4_ORYSJ	1.030e-38	39.11	Allene oxide synthase 4 OS=Oryza sativa subsp. jap...	[more]

BLAST of GR396736 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q7X9B3_MEDTR	1.572e-114	84.44	9/13 hydroperoxide lyase OS=Medicago truncatula GN...	[more]
B7FLW4_MEDTR	1.127e-112	83.56	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q7X9B2_MEDTR	4.618e-82	63.72	9/13 hydroperoxide lyase OS=Medicago truncatula GN...	[more]
Q2LAJ5_SOYBN	1.065e-78	60.18	Cytochrome P450 monooxygenase CYP74A1 OS=Glycine m...	[more]
B9P6E9_POPTR	1.140e-72	56.25	Cytochrome P450 OS=Populus trichocarpa GN=CYP74C6v...	[more]
B9H0K5_POPTR	1.140e-72	56.25	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HRU0_POPTR	1.260e-71	56.70	Cytochrome P450 OS=Populus trichocarpa GN=CYP74C8 ...	[more]
B9H0K6_POPTR	7.647e-69	54.46	Cytochrome P450 OS=Populus trichocarpa GN=CYP74C7-...	[more]
A9PC92_POPTR	7.647e-69	54.46	Putative uncharacterized protein OS=Populus tricho...	[more]
Q7XB42_PRUDU	3.552e-66	55.11	Cytochrome P450 OS=Prunus dulcis GN=cyp74C5 PE=3 S...	[more]

BLAST of GR396736 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G42650.1	1.994e-59	48.65	\| Symbols: AOS, CYP74A, DDE2 \| allene oxide syntha...	[more]
AT4G15440.1	2.088e-16	33.33	\| Symbols: HPL1, CYP74B2 \| hydroperoxide lyase 1 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 23..34 score: NAcoord: 44..62 score: NAcoord: 100..108 score: NAcoord: 119..136 score
None	No IPR available	SEG	seg	seg	coord: 20..33 score
None	No IPR available	SEG	seg	seg	coord: 6..17 score
None	No IPR available	SEG	seg	seg	coord: 9..20 score
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 16..195 score: 8.7
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 13..238 score: 6.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR396736 ID=GR396736; Name=GR396736; organism=Cicer arietinum; type=EST; length=728bp
GATCAACAAAAATGGCTTCATCCTCTGAACCTTCCTCCACCACCCTCCCC
TTGAAACCAATCCCTGGAAGCTACGGTCTTCCCGTCATAGGACCCTTACA
TGACAGACATGACTATTTCTACAACGAAGGACGCGACAAATTCTTCGCTT
CAAGAGTCCAAAAGTACAATTCCACCGTCATCAAAGTCAACATGCCACCT
GGTGGTTTCATCGCTCCAAACCCTAAAGTCATCGCTCTTCTCGACGGTGT
TTCTTTCCCTATTCTTTTCGACAACTCCAAAGTCGATAAACGTGACGTTC
TCGACGGAACTTTCATGCCTTCCACCGATTACTTCGGCGGTTACCGCACC
TGCGCTTTTCAAGACACAACTGAACCTTCCCATAAACTCATCAAAACCTT
CATCTTCCACATCCTTTCATCAAAACACGAAACCTTCATACCTCTCTTCA
GCTCCAACATCTCCGATCACTTCACCGACCTCGAGAAAGACCTCGCCGGA
AAACATGCTAAAGCGAGTTTCAACTCCTCTATCGGCAACGCCACGTTCAA
CTTCCTCTTCCGTCTTTTAACAAACAAAAACCCAACCGAAACAAAAATCG
GCGACTCCGGTCCCACACTCGTTCAAACATGGCTTGCGGCTCAGCTTGCG
CCGTTAGCCACAGCCGGTCTTCCGAAAATATTTAACTATATAGAAGATAT
TCTGATCAGAACAATTCCGATCCCGGCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

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