GR399733

EST Overview


Name	GR399733
Unique Name	GR399733
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	698

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR399733 vs. SwissProt
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.990e-40
Identity = 74/85 (87.06%), Postives = 81/85 (95.29%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+GYYKICRGRN+CGVDSMVSTVAA  +++
Sbjct:  279 MQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQSNH 363

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BLAST of GR399733 vs. SwissProt
Match: PAPA5_CARPA (Cysteine proteinase (Fragment) OS=Carica papaya PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 9.002e-37
Identity = 69/85 (81.18%), Postives = 80/85 (94.12%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ G+APIRLKEKPYW+IKNSWG+NWGE GYYKICRGRNICGVDSMVSTVAAVH ++
Sbjct:   12 MQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGYAPIRLKEKPYWVIKNSWGENWGENGYYKICRGRNICGVDSMVSTVAAVHTTS 95

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BLAST of GR399733 vs. SwissProt
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.100e-34
Identity = 70/87 (80.46%), Postives = 77/87 (88.51%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFG-NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRG---RNICGVDSMVSTVAAVHAS 249
            MQTY+ GVSCPYIC +  LDHGVLLVG+G +GFAPIRLK+KPYWIIKNSWG+NWGE GYYKICRG   RN CGVDSMVSTV+AVHAS
Sbjct:  284 MQTYIGGVSCPYICGR-HLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVSTVSAVHAS 369

[Close]

BLAST of GR399733 vs. SwissProt
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.997e-32
Identity = 63/80 (78.75%), Postives = 71/80 (88.75%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAA 237
            MQTY+ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE G+YKIC+GRNICGVDSMVSTVAA
Sbjct:  283 MQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTVAA 361

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BLAST of GR399733 vs. SwissProt
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 137.887 bits (346), Expect = 5.112e-32
Identity = 62/80 (77.50%), Postives = 74/80 (92.50%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAA 237
            MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE G+YKIC+GRNICGVDS+VSTVAA
Sbjct:  280 MQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

[Close]

BLAST of GR399733 vs. SwissProt
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 8.465e-19
Identity = 42/78 (53.85%), Postives = 53/78 (67.95%), Query Frame = 1

Query:    1 MQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVST 228
            MQ Y  GVS P+  +C+K  LDHGVL+VG+G    P   K  PYWI+KNSWG  WGE+GYY++ RG N CGV  M ++
Sbjct:  533 MQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATS 610

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BLAST of GR399733 vs. SwissProt
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.678e-15
Identity = 38/78 (48.72%), Postives = 50/78 (64.10%), Query Frame = 1

Query:    1 MQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVST 228
            MQ Y  G++ P+  +C+   +DH VLLVG+GN          PYW IKNSWG +WGEEGYY + RG   CGV++M S+
Sbjct:  387 MQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNR------SNIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASS 458

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BLAST of GR399733 vs. SwissProt
Match: CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.158e-14
Identity = 37/83 (44.58%), Postives = 55/83 (66.27%), Query Frame = 1

Query:    1 MQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVH 243
            +Q Y  G+S P+   C+K  LDH VLLVG+G     +  K +P+WI+KNSWG  WGE GY+++ RG   CG+++ V+T A ++
Sbjct:  243 LQFYQHGISHPWWIFCSKYLLDHAVLLVGYG-----VSEKNEPFWIVKNSWGVEWGENGYFRMYRGDGSCGINT-VATSAMIY 319

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BLAST of GR399733 vs. SwissProt
Match: CATF_HUMAN (Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.680e-14
Identity = 37/78 (47.44%), Postives = 50/78 (64.10%), Query Frame = 1

Query:    1 MQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVST 228
            MQ Y  G+S P   +C+   +DH VLLVG+GN        + P+W IKNSWG +WGE+GYY + RG   CGV++M S+
Sbjct:  409 MQFYRHGISRPLRPLCSPWLIDHAVLLVGYGNR------SDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASS 480

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BLAST of GR399733 vs. SwissProt
Match: CYSP1_DICDI (Cysteine proteinase 1 OS=Dictyostelium discoideum GN=cprA PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 8.199e-14
Identity = 37/75 (49.33%), Postives = 48/75 (64.00%), Query Frame = 1

Query:    4 QTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVST 228
            Q Y+ GV     C  + LDHG+L+VG+       R K  PYWI+KNSWG +WGE+GY  + RG+N CGV + VST
Sbjct:  268 QFYIGGVF-DIPCNPNSLDHGILIVGYSAKNTIFR-KNMPYWIVKNSWGADWGEQGYIYLRRGKNTCGVSNFVST 340

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BLAST of GR399733 vs. TrEMBL
Match: O81930_CICAR (Cysteine proteinase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.840e-45
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 255
            MQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN
Sbjct:  278 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 362

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BLAST of GR399733 vs. TrEMBL
Match: Q8GV06_VICFA (Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.509e-39
Identity = 74/85 (87.06%), Postives = 81/85 (95.29%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+GYYKICRGRN+CGVDSMVSTVAA  ++N
Sbjct:  279 MQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQSNN 363

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BLAST of GR399733 vs. TrEMBL
Match: Q41671_VICFA (Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYSPRO PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.509e-39
Identity = 74/85 (87.06%), Postives = 81/85 (95.29%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+GYYKICRGRN+CGVDSMVSTVAA  ++N
Sbjct:  279 MQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQSNN 363

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BLAST of GR399733 vs. TrEMBL
Match: Q41698_VICSA (Cysteine proteinase OS=Vicia sativa PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 9.781e-39
Identity = 73/85 (85.88%), Postives = 80/85 (94.12%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQ YMSGVSCPY+CAK+RLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+GYYKICRGRN+CGVDSMVSTVAA  ++N
Sbjct:  274 MQAYMSGVSCPYVCAKARLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQSNN 358

[Close]

BLAST of GR399733 vs. TrEMBL
Match: Q9STA4_MEDSA (Cysteine protease (Fragment) OS=Medicago sativa GN=cyp15a PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.668e-38
Identity = 74/83 (89.16%), Postives = 80/83 (96.39%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHA 246
            MQTYMSGVSCP+ICAK+RLDHGVLLVGFG+G +APIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRN+CGVDSMVSTVAA  +
Sbjct:  127 MQTYMSGVSCPHICAKARLDHGVLLVGFGSGGYAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNVCGVDSMVSTVAAAQS 209

[Close]

BLAST of GR399733 vs. TrEMBL
Match: C6TDZ7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 7.010e-37
Identity = 71/86 (82.56%), Postives = 79/86 (91.86%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 255
            MQTY+ GVSCPYIC K  LDHGVLLVG+G+G +APIR KEKPYWIIKNSWG++WGEEGYYKICRGRN+CGVDSMVSTVAA+H SNN
Sbjct:  282 MQTYIGGVSCPYICGK-HLDHGVLLVGYGSGAYAPIRFKEKPYWIIKNSWGESWGEEGYYKICRGRNVCGVDSMVSTVAAIHVSNN 366

[Close]

BLAST of GR399733 vs. TrEMBL
Match: B5SV85_ROBPS (Cysteine protease-like protein (Fragment) OS=Robinia pseudoacacia PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.664e-36
Identity = 71/86 (82.56%), Postives = 79/86 (91.86%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 255
            MQTY+ GVSCPYIC K+ LDHGVLLVG+G  G+APIRLKEKPYWIIKNSWG++WGE GYYKICRGRN+CGVDSMVSTVAAVH S+N
Sbjct:  251 MQTYIGGVSCPYICGKN-LDHGVLLVGYGKAGYAPIRLKEKPYWIIKNSWGESWGENGYYKICRGRNVCGVDSMVSTVAAVHTSHN 335

[Close]

BLAST of GR399733 vs. TrEMBL
Match: A9UFX8_VITVI (Cysteine protease OS=Vitis vinifera GN=CysP PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 3.479e-36
Identity = 69/86 (80.23%), Postives = 81/86 (94.19%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 255
            MQTY+ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+K+KPYWIIKNSWG+NWGE G+YKICRGRN+CGVDSMVSTVAAVH ++N
Sbjct:  293 MQTYVGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKDKPYWIIKNSWGENWGENGFYKICRGRNVCGVDSMVSTVAAVHTTSN 377

[Close]

BLAST of GR399733 vs. TrEMBL
Match: Q5Y804_PETHY (Cysteine proteinase (Fragment) OS=Petunia hybrida GN=CP5 PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 5.934e-36
Identity = 69/84 (82.14%), Postives = 77/84 (91.67%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTY+ GVSCPYIC+K R DHGVLLVG+G+GFAPIR+KEKPYWIIKNSWG+ WGE GYYKICRGRN+CGVDSMVSTVAAV  S+
Sbjct:  108 MQTYVGGVSCPYICSK-RQDHGVLLVGYGSGFAPIRMKEKPYWIIKNSWGEKWGESGYYKICRGRNVCGVDSMVSTVAAVSTSS 190

[Close]

BLAST of GR399733 vs. TrEMBL
Match: B9RHA4_RICCO (Cysteine protease, putative OS=Ricinus communis GN=RCOM_1449120 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.727e-35
Identity = 70/85 (82.35%), Postives = 79/85 (92.94%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASN 252
            MQTY+ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+KEKPYWIIKNSWG+NWGE GYYKICRGRNICGVDSMVSTVAAV  ++
Sbjct:  289 MQTYIGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGENWGESGYYKICRGRNICGVDSMVSTVAAVQTAS 372

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT4G16190.1 (| Symbols: | Papain family cysteine protease | chr4:9171512-9172877 FORWARD LENGTH=373)

HSP 1 Score: 142.895 bits (359), Expect = 1.599e-34
Identity = 67/85 (78.82%), Postives = 76/85 (89.41%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRG-RNICGVDSMVSTVAAVHAS 249
            MQTY+ GVSCPY+C+KS+ DHGVLLVGFG+ G+APIRLKEKPYWIIKNSWG  WGE GYYKICRG  N+CG+D+MVSTVAAVH S
Sbjct:  288 MQTYIGGVSCPYVCSKSQ-DHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVSTVAAVHTS 371

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT4G39090.1 (| Symbols: RD19, RD19A | Papain family cysteine protease | chr4:18215826-18217326 REVERSE LENGTH=368)

HSP 1 Score: 138.658 bits (348), Expect = 3.015e-33
Identity = 63/80 (78.75%), Postives = 71/80 (88.75%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAA 237
            MQTY+ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE G+YKIC+GRNICGVDSMVSTVAA
Sbjct:  283 MQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTVAA 361

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT2G21430.1 (| Symbols: | Papain family cysteine protease | chr2:9171964-9173301 REVERSE LENGTH=361)

HSP 1 Score: 137.887 bits (346), Expect = 5.144e-33
Identity = 62/80 (77.50%), Postives = 74/80 (92.50%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAA 237
            MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE G+YKIC+GRNICGVDS+VSTVAA
Sbjct:  280 MQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT3G54940.2 (| Symbols: | Papain family cysteine protease | chr3:20354402-20356127 FORWARD LENGTH=367)

HSP 1 Score: 126.331 bits (316), Expect = 1.549e-29
Identity = 54/79 (68.35%), Postives = 67/79 (84.81%), Query Frame = 1

Query:    1 MQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVA 234
            MQTY+ GVSCP IC+K  ++HGVLLVG+G+ GF+ +RL  KPYWIIKNSWG+ WGE GYYK+CRG +ICG++SMVS VA
Sbjct:  284 MQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT5G60360.1 (| Symbols: SAG2, AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=358)

HSP 1 Score: 65.0846 bits (157), Expect = 4.237e-11
Identity = 32/76 (42.11%), Postives = 46/76 (60.53%), Query Frame = 1

Query:   10 YMSGVSCPYICAKSRLD--HGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVS 225
            Y SGV     C  + +D  H VL VG+G  +G         PYW+IKNSWG +WG++GY+K+  G+N+CG+ +  S
Sbjct:  286 YKSGVYTDSHCGSTPMDVNHAVLAVGYGVEDGV--------PYWLIKNSWGADWGDKGYFKMEMGKNMCGIATCAS 353

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT3G45310.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=358)

HSP 1 Score: 63.929 bits (154), Expect = 9.439e-11
Identity = 30/69 (43.48%), Postives = 40/69 (57.97%), Query Frame = 1

Query:   10 YMSGVSCPYICAKSRLD--HGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGV 210
            Y  GV     C  + +D  H VL VG+G         + PYW+IKNSWG  WG+ GY+K+  G+N+CGV
Sbjct:  286 YKKGVFTSNTCGNTPMDVNHAVLAVGYGVE------DDVPYWLIKNSWGGEWGDNGYFKMEMGKNMCGV 348

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT5G60360.3 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282157 FORWARD LENGTH=361)

HSP 1 Score: 63.1586 bits (152), Expect = 1.610e-10
Identity = 31/70 (44.29%), Postives = 43/70 (61.43%), Query Frame = 1

Query:   10 YMSGVSCPYICAKSRLD--HGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICG 207
            Y SGV     C  + +D  H VL VG+G  +G         PYW+IKNSWG +WG++GY+K+  G+N+CG
Sbjct:  286 YKSGVYTDSHCGSTPMDVNHAVLAVGYGVEDGV--------PYWLIKNSWGADWGDKGYFKMEMGKNMCG 347

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT3G19390.1 (| Symbols: | Granulin repeat cysteine protease family protein | chr3:6723024-6724768 FORWARD LENGTH=452)

HSP 1 Score: 61.2326 bits (147), Expect = 6.118e-10
Identity = 39/89 (43.82%), Postives = 47/89 (52.81%), Query Frame = 1

Query:    4 QTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNI------CGVDSMVSTVAAVHASN 252
            Q Y SGV     C  S LDHGV+ VG+G+         + YWI++NSWG NWGE GY+K+   RNI      CGV  M S       SN
Sbjct:  273 QLYTSGVFTG-TCGTS-LDHGVVAVGYGSEGG------QDYWIVRNSWGSNWGESGYFKL--ERNIKESSGKCGVAMMASYPTKSSGSN 351

[Close]

BLAST of GR399733 vs. TAIR peptide
Match: AT5G60360.2 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=357)

HSP 1 Score: 60.8474 bits (146), Expect = 7.991e-10
Identity = 30/69 (43.48%), Postives = 42/69 (60.87%), Query Frame = 1

Query:   10 YMSGVSCPYICAKSRLD--HGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIC 204
            Y SGV     C  + +D  H VL VG+G  +G         PYW+IKNSWG +WG++GY+K+  G+N+C
Sbjct:  286 YKSGVYTDSHCGSTPMDVNHAVLAVGYGVEDGV--------PYWLIKNSWGADWGDKGYFKMEMGKNMC 346

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BLAST of GR399733 vs. TAIR peptide
Match: AT3G45310.2 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=357)

HSP 1 Score: 60.077 bits (144), Expect = 1.363e-9
Identity = 28/67 (41.79%), Postives = 38/67 (56.72%), Query Frame = 1

Query:   10 YMSGVSCPYICAKSRLD--HGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIC 204
            Y  GV     C  + +D  H VL VG+G         + PYW+IKNSWG  WG+ GY+K+  G+N+C
Sbjct:  286 YKKGVFTSNTCGNTPMDVNHAVLAVGYGVE------DDVPYWLIKNSWGGEWGDNGYFKMEMGKNMC 346

The following BLAST results are available for this feature:

BLAST of GR399733 vs. SwissProt
BLAST of GR399733 vs. TrEMBL
BLAST of GR399733 vs. TAIR peptide

BLAST of GR399733 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CYSP_PEA	2.990e-40	87.06	Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1	[more]
PAPA5_CARPA	9.002e-37	81.18	Cysteine proteinase (Fragment) OS=Carica papaya PE...	[more]
CYSP1_MAIZE	1.100e-34	80.46	Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=...	[more]
RD19A_ARATH	2.997e-32	78.75	Cysteine proteinase RD19a OS=Arabidopsis thaliana ...	[more]
A494_ARATH	5.112e-32	77.50	Probable cysteine proteinase A494 OS=Arabidopsis t...	[more]
CPR1_DROME	8.465e-19	53.85	Putative cysteine proteinase CG12163 OS=Drosophila...	[more]
CATF_MOUSE	5.678e-15	48.72	Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1	[more]
CATL_SCHMA	2.158e-14	44.58	Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=...	[more]
CATF_HUMAN	3.680e-14	47.44	Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1	[more]
CYSP1_DICDI	8.199e-14	49.33	Cysteine proteinase 1 OS=Dictyostelium discoideum ...	[more]

BLAST of GR399733 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O81930_CICAR	1.840e-45	100.00	Cysteine proteinase OS=Cicer arietinum PE=2 SV=1	[more]
Q8GV06_VICFA	1.509e-39	87.06	Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=...	[more]
Q41671_VICFA	1.509e-39	87.06	Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYS...	[more]
Q41698_VICSA	9.781e-39	85.88	Cysteine proteinase OS=Vicia sativa PE=2 SV=1	[more]
Q9STA4_MEDSA	1.668e-38	89.16	Cysteine protease (Fragment) OS=Medicago sativa GN...	[more]
C6TDZ7_SOYBN	7.010e-37	82.56	Putative uncharacterized protein OS=Glycine max PE...	[more]
B5SV85_ROBPS	2.664e-36	82.56	Cysteine protease-like protein (Fragment) OS=Robin...	[more]
A9UFX8_VITVI	3.479e-36	80.23	Cysteine protease OS=Vitis vinifera GN=CysP PE=2 S...	[more]
Q5Y804_PETHY	5.934e-36	82.14	Cysteine proteinase (Fragment) OS=Petunia hybrida ...	[more]
B9RHA4_RICCO	1.727e-35	82.35	Cysteine protease, putative OS=Ricinus communis GN...	[more]

BLAST of GR399733 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G16190.1	1.599e-34	78.82	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT4G39090.1	3.015e-33	78.75	\| Symbols: RD19, RD19A \| Papain family cysteine pr...	[more]
AT2G21430.1	5.144e-33	77.50	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT3G54940.2	1.549e-29	68.35	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT5G60360.1	4.237e-11	42.11	\| Symbols: SAG2, AALP, ALP \| aleurain-like proteas...	[more]
AT3G45310.1	9.439e-11	43.48	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT5G60360.3	1.610e-10	44.29	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT3G19390.1	6.118e-10	43.82	\| Symbols: \| Granulin repeat cysteine protease fa...	[more]
AT5G60360.2	7.991e-10	43.48	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT3G45310.2	1.363e-9	41.79	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 19..29 score
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 42..61 score
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 2..76 score: 7.8
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 1..84 score: 6.7
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 2..78 score: 6.9
None	No IPR available	PANTHER	PTHR12411:SF42	CYSTEINE PROTEASE	coord: 1..84 score: 6.7
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..79 score: 8.5
None	No IPR available	SEG	seg	seg	coord: 49..64 score
None	No IPR available	SEG	seg	seg	coord: 18..31 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR399733 ID=GR399733; Name=GR399733; organism=Cicer arietinum; type=EST; length=698bp
ATGCAAACATATATGAGTGGTGTCTCATGCCCATACATCTGTGCAAAATC
GCGTTTGGATCATGGGGTTCTTCTAGTTGGTTTTGGAAATGGTTTTGCTC
CCATCCGATTGAAGGAAAAGCCTTACTGGATCATAAAGAATTCATGGGGA
CAGAATTGGGGTGAGGAAGGATATTACAAGATCTGCAGAGGTCGAAATAT
ATGTGGAGTGGATTCAATGGTCTCAACTGTAGCTGCAGTTCATGCATCCA
ACAATTAATAATACGGAGATGCTGGTTCTGGTGGTCTCATCCTATGTGAC
ACGACTCTAGTTCAATTTGTGTAAATATATAATCATGAAACTGTGAATTT
ATCTTCTGGTTCTGTGCAAACTATCTCACTATAGGATCTGAAAATTACCC
CAATCACGTTATTTTATGAATATGATATATTTTATATGTCTTTTTACTCC
AGTGTTGTGCTAACTATATTCTTGATAACACCGGCACATATTTGTTATTA
TCATATTAATAAATTGTATACAATAACACTCTTATCATTCCATTTTCACT
CAATTATTTTCTTCAAAATATTATTATATTCAACAACTAATAGTTTGTTT
TTCTTTTTTTATTAATTACTCTAATCAACATTTATTTATTCCTTTTTAAT
TCTATTATTTATAATTTTAACACTCTTCAATTAAATATCCTATGTATC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004197	cysteine-type endopeptidase activity
GO:0008234	cysteine-type peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A

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