GR399795

EST Overview


Name	GR399795
Unique Name	GR399795
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	732

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR399795 vs. SwissProt
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.499e-37
Identity = 71/86 (82.56%), Postives = 79/86 (91.86%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV +++DQI ANLVKNGP AV INAAW+QT+MSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+
Sbjct:  250 FSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQ 335

HSP 2 Score: 54.6842 bits (130), Expect = 6.144e-7
Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = -2

Query:  291 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAAVHASN 407
            K   G   G  GYYKICRGRN+CGVDSMVSTVAA  +++
Sbjct:  325 KNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQSNH 363

[Close]

BLAST of GR399795 vs. SwissProt
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 4.744e-30
Identity = 60/85 (70.59%), Postives = 74/85 (87.06%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFG-NGFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV V++ QI ANL+K+GP A+ INAA++QT++ GVSCPYIC +  LDHGVLLVG+G +GFAPIRLK+KPYWIIKNSWG+NWGE
Sbjct:  255 FSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICGR-HLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGE 338

HSP 2 Score: 21.557 bits (44), Expect = 4.744e-30
Identity = 7/12 (58.33%), Postives = 10/12 (83.33%), Query Frame = -2

Query:  642 DGSCKFTKTRMV 677
            DG CKF K+++V
Sbjct:  238 DGKCKFDKSKIV 249

[Close]

BLAST of GR399795 vs. SwissProt
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 129.798 bits (325), Expect = 1.503e-29
Identity = 59/85 (69.41%), Postives = 74/85 (87.06%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV + +DQI ANL+KNGP AV INAA++QT++ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE
Sbjct:  251 FSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGE 334

[Close]

BLAST of GR399795 vs. SwissProt
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 9.741e-29
Identity = 57/85 (67.06%), Postives = 71/85 (83.53%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFG-NGFAPIRLKEKPYWIIKNSWGQNWGE 628
            F V+ ++++QI ANLVKNGP AV INA ++QT++ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE
Sbjct:  254 FSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGE 337

[Close]

BLAST of GR399795 vs. SwissProt
Match: PAPA5_CARPA (Cysteine proteinase (Fragment) OS=Carica papaya PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.114e-24
Identity = 50/67 (74.63%), Postives = 62/67 (92.54%), Query Frame = -3

Query:  377 GPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 574
            GP AV INAA++QT++ GVSCPYIC++ RL+HGVLLVG+G+ G+APIRLKEKPYW+IKNSWG+NWGE
Sbjct:    1 GPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGYAPIRLKEKPYWVIKNSWGENWGE 66

HSP 2 Score: 60.077 bits (144), Expect = 1.462e-8
Identity = 28/39 (71.79%), Postives = 30/39 (76.92%), Query Frame = -2

Query:  291 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAAVHASN 407
            K   G   G  GYYKICRGRNICGVDSMVSTVAAVH ++
Sbjct:   57 KNSWGENWGENGYYKICRGRNICGVDSMVSTVAAVHTTS 95

[Close]

BLAST of GR399795 vs. SwissProt
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 1.783e-14
Identity = 38/77 (49.35%), Postives = 50/77 (64.94%), Query Frame = -3

Query:  362 LVKNGPFAVVINAAWVQTFMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEDITR 586
            L+ NGP ++ INA  +Q +  GVS P+  +C+K  LDHGVL+VG+G    P   K  PYWI+KNSWG  WGE+   R
Sbjct:  518 LLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYR 594

[Close]

BLAST of GR399795 vs. SwissProt
Match: CYSP_TRYCR (Cruzipain OS=Trypanosoma cruzi PE=1 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.278e-12
Identity = 40/89 (44.94%), Postives = 51/89 (57.30%), Query Frame = -3

Query:  353 VPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEDITRSAE 619
            +P ++ QI A L  NGP AV ++A+   T+  GV     C   +LDHGVLLVG+ +  A       PYWIIKNSW   WGEE   R A+
Sbjct:  240 LPQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTS--CVSEQLDHGVLLVGYNDSAAV------PYWIIKNSWTTQWGEEGYIRIAK 320

[Close]

BLAST of GR399795 vs. SwissProt
Match: CATW_FELCA (Cathepsin W OS=Felis catus GN=CTSW PE=2 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 2.333e-12
Identity = 34/97 (35.05%), Postives = 46/97 (47.42%), Query Frame = -3

Query:  380 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGV--SCPYICAKSRLDHGVLLVGFGN------------GFAPIRLKEKPYWIIKNSWGQNWG 628
            F ++P  + +I   L   GP  V IN   ++ +  GV  + P  C    +DH VLLVGFG             G  P   +  P+WI+KNSWG  WG
Sbjct:  240 FIMLPDNEQKIAWYLATQGPITVTINMKLLKLYKKGVIEATPTSCDPFLVDHSVLLVGFGKSESVADRRAGAAGAQPQSRRSIPFWILKNSWGTKWG 336

HSP 2 Score: 32.3426 bits (72), Expect = 2.333e-12
Identity = 12/25 (48.00%), Postives = 17/25 (68.00%), Query Frame = -2

Query:  333 KEFMGTELG*GGYYKICRGRNICGV 407
            K   GT+ G GGY+++ RG N CG+
Sbjct:  328 KNSWGTKWGXGGYFRLYRGNNTCGI 352

[Close]

BLAST of GR399795 vs. SwissProt
Match: CYSP_TRYBB (Cysteine proteinase OS=Trypanosoma brucei brucei PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.719e-12
Identity = 35/86 (40.70%), Postives = 48/86 (55.81%), Query Frame = -3

Query:  362 VPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEDITR 619
            +P ++D I A L +NGP A+ ++A     +  G+     C   +LDHGVLLVG+ +   P      PYWIIKNSW   WGE+   R
Sbjct:  243 LPQDEDAIAAYLAENGPLAIAVDAESFMDYNGGILTS--CTSKQLDHGVLLVGYNDNSNP------PYWIIKNSWSNMWGEDGYIR 320

[Close]

BLAST of GR399795 vs. SwissProt
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.343e-12
Identity = 35/80 (43.75%), Postives = 50/80 (62.50%), Query Frame = -3

Query:  374 KDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEE 607
            +++I A L + GP +V INA  +Q +  G++ P+  +C+   +DH VLLVG+GN          PYW IKNSWG +WGEE
Sbjct:  365 ENKIAAWLAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNR------SNIPYWAIKNSWGSDWGEE 438

[Close]

BLAST of GR399795 vs. TrEMBL
Match: O24322_PHAVU (Cysteine proteinase OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 2.059e-40
Identity = 58/84 (69.05%), Postives = 71/84 (84.52%), Query Frame = -3

Query:  380 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWG 628
            + V+ ++++QI ANLVKNGP AV INA ++QT++ GVSCPYIC K  LDHGVLLVG+G G +APIR KEKPYWIIKNSWG+NWG
Sbjct:  252 YSVISLDEEQIAANLVKNGPLAVAINAVYMQTYVGGVSCPYICGK-HLDHGVLLVGYGEGAYAPIRFKEKPYWIIKNSWGENWG 334

HSP 2 Score: 60.077 bits (144), Expect = 2.059e-40
Identity = 27/38 (71.05%), Postives = 29/38 (76.32%), Query Frame = -2

Query:  294 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAAVHAS 407
            K   G   G  GYYKICRGRN+CGVDSMVSTV A+HAS
Sbjct:  326 KNSWGENWGGNGYYKICRGRNVCGVDSMVSTVGAIHAS 363

HSP 3 Score: 22.3274 bits (46), Expect = 2.059e-40
Identity = 7/12 (58.33%), Postives = 11/12 (91.67%), Query Frame = -2

Query:  645 RDGSCKFTKTRM 680
            RDG+CKF K+++
Sbjct:  234 RDGTCKFDKSKI 245

[Close]

BLAST of GR399795 vs. TrEMBL
Match: O81930_CICAR (Cysteine proteinase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.284e-39
Identity = 76/85 (89.41%), Postives = 80/85 (94.12%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV V++DQI ANLVKNGP AV INAAW+QT+MSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEE
Sbjct:  249 FSVVSVDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEE 333

HSP 2 Score: 66.2402 bits (160), Expect = 3.084e-9
Identity = 32/40 (80.00%), Postives = 32/40 (80.00%), Query Frame = -2

Query:  288 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAAVHASNN 407
            K   G   G  GYYKICRGRNICGVDSMVSTVAAVHASNN
Sbjct:  323 KNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAAVHASNN 362

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q96455_SOYBN (Thiol protease isoform A (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.479e-36
Identity = 58/84 (69.05%), Postives = 70/84 (83.33%), Query Frame = -3

Query:  371 VPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEED 619
            V ++++QI ANLVKNGP AV INA ++QT++ GVSCPYIC K  LDHGVLLVG+G G +APIR K KPYWIIKNSWG++WGE D
Sbjct:  207 VSLDEEQIAANLVKNGPLAVAINAVFMQTYVGGVSCPYICGK-HLDHGVLLVGYGEGRYAPIRFKNKPYWIIKNSWGESWGEND 289

HSP 2 Score: 50.8322 bits (120), Expect = 1.479e-36
Identity = 21/29 (72.41%), Postives = 28/29 (96.55%), Query Frame = -2

Query:  288 GYYKICRGRNICGVDSMVSTVAAVHASNN 374
            GY +ICRGRN+CGVD+MVSTVAA++AS++
Sbjct:  290 GYDEICRGRNVCGVDAMVSTVAAIYASSH 318

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q9STA4_MEDSA (Cysteine protease (Fragment) OS=Medicago sativa GN=cyp15a PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 5.048e-36
Identity = 71/86 (82.56%), Postives = 80/86 (93.02%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV +++DQI ANLVKNGP AV INAAW+QT+MSGVSCP+ICAK+RLDHGVLLVGFG+ G+APIRLKEKPYWIIKNSWGQNWGEE
Sbjct:   98 FSVVSLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPHICAKARLDHGVLLVGFGSGGYAPIRLKEKPYWIIKNSWGQNWGEE 183

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q8GV06_VICFA (Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.918e-35
Identity = 69/86 (80.23%), Postives = 79/86 (91.86%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV ++++QI ANLVKNGP AV INAAW+QT+MSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+
Sbjct:  250 FSVVSLDEEQIAANLVKNGPLAVGINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQ 335

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q41671_VICFA (Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYSPRO PE=2 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.918e-35
Identity = 69/86 (80.23%), Postives = 79/86 (91.86%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV ++++QI ANLVKNGP AV INAAW+QT+MSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+
Sbjct:  250 FSVVSLDEEQIAANLVKNGPLAVGINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQ 335

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q41698_VICSA (Cysteine proteinase OS=Vicia sativa PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 5.582e-35
Identity = 68/86 (79.07%), Postives = 78/86 (90.70%), Query Frame = -3

Query:  374 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEE 628
            F VV ++++QI ANLVKNGP AV INAAW+Q +MSGVSCPY+CAK+RLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+
Sbjct:  245 FSVVSLDEEQIAANLVKNGPLAVAINAAWMQAYMSGVSCPYVCAKARLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQ 330

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q5ZF63_PLAMJ (Cysteine protease 2 (Fragment) OS=Plantago major GN=cpr2 PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 4.890e-31
Identity = 61/84 (72.62%), Postives = 73/84 (86.90%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV +++DQI ANLVK GP AV INAAW+QT++ GVSCPYIC KS LDHGVL+VG+G G+AP+RLK KPYWIIKNSWG++WGE
Sbjct:  133 FSVVSIDEDQIAANLVKYGPLAVGINAAWMQTYIGGVSCPYICGKS-LDHGVLIVGYGTGYAPVRLKNKPYWIIKNSWGESWGE 215

[Close]

BLAST of GR399795 vs. TrEMBL
Match: B9RHA4_RICCO (Cysteine protease, putative OS=Ricinus communis GN=RCOM_1449120 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.089e-30
Identity = 63/85 (74.12%), Postives = 76/85 (89.41%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV +++DQI ANLVKNGP AV INA ++QT++ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+KEKPYWIIKNSWG+NWGE
Sbjct:  260 FSVVSLDEDQIAANLVKNGPLAVAINAVFMQTYIGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGENWGE 343

[Close]

BLAST of GR399795 vs. TrEMBL
Match: Q5Y804_PETHY (Cysteine proteinase (Fragment) OS=Petunia hybrida GN=CP5 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.423e-30
Identity = 61/84 (72.62%), Postives = 74/84 (88.10%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV ++++QI ANLVKNGP AV INA ++QT++ GVSCPYIC+K R DHGVLLVG+G+GFAPIR+KEKPYWIIKNSWG+ WGE
Sbjct:   79 FSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYVGGVSCPYICSK-RQDHGVLLVGYGSGFAPIRMKEKPYWIIKNSWGEKWGE 161

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT4G16190.1 (| Symbols: | Papain family cysteine protease | chr4:9171512-9172877 FORWARD LENGTH=373)

HSP 1 Score: 131.724 bits (330), Expect = 3.946e-31
Identity = 60/85 (70.59%), Postives = 73/85 (85.88%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV  ++DQI ANLV++GP A+ INA W+QT++ GVSCPY+C+KS+ DHGVLLVGFG+ G+APIRLKEKPYWIIKNSWG  WGE
Sbjct:  259 FSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSKSQ-DHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGE 342

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT2G21430.1 (| Symbols: | Papain family cysteine protease | chr2:9171964-9173301 REVERSE LENGTH=361)

HSP 1 Score: 129.798 bits (325), Expect = 1.500e-30
Identity = 59/85 (69.41%), Postives = 74/85 (87.06%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 628
            F VV + +DQI ANL+KNGP AV INAA++QT++ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE
Sbjct:  251 FSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGE 334

HSP 2 Score: 50.8322 bits (120), Expect = 8.853e-7
Identity = 23/34 (67.65%), Postives = 26/34 (76.47%), Query Frame = -2

Query:  306 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAA 407
            K   G   G  G+YKIC+GRNICGVDS+VSTVAA
Sbjct:  325 KNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT4G39090.1 (| Symbols: RD19, RD19A | Papain family cysteine protease | chr4:18215826-18217326 REVERSE LENGTH=368)

HSP 1 Score: 127.102 bits (318), Expect = 9.720e-30
Identity = 57/85 (67.06%), Postives = 71/85 (83.53%), Query Frame = -3

Query:  377 FRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFG-NGFAPIRLKEKPYWIIKNSWGQNWGE 628
            F V+ ++++QI ANLVKNGP AV INA ++QT++ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE
Sbjct:  254 FSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGE 337

HSP 2 Score: 51.6026 bits (122), Expect = 5.190e-7
Identity = 24/34 (70.59%), Postives = 26/34 (76.47%), Query Frame = -2

Query:  306 KEFMGTELG*GGYYKICRGRNICGVDSMVSTVAA 407
            K   G   G  G+YKIC+GRNICGVDSMVSTVAA
Sbjct:  328 KNSWGETWGENGFYKICKGRNICGVDSMVSTVAA 361

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT3G54940.2 (| Symbols: | Papain family cysteine protease | chr3:20354402-20356127 FORWARD LENGTH=367)

HSP 1 Score: 119.013 bits (297), Expect = 2.647e-27
Identity = 51/87 (58.62%), Postives = 71/87 (81.61%), Query Frame = -3

Query:  377 LKFRVVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGE 634
            L F  +P++++QI ANLV++GP AV +NA ++QT++ GVSCP IC+K  ++HGVLLVG+G+ GF+ +RL  KPYWIIKNSWG+ WGE
Sbjct:  253 LNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGE 339

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT3G43960.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:15774122-15775628 REVERSE LENGTH=376)

HSP 1 Score: 55.4546 bits (132), Expect = 3.594e-8
Identity = 35/84 (41.67%), Postives = 45/84 (53.57%), Query Frame = -3

Query:  377 VVPVEKDQIGANLVKNGPFAVVINAAWVQTFMSGVSCPYICAKSRL--DHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGE 622
            VVPV  +      V   P +V+I+AA +  + SGV   Y  A S L  DH VL+VG+G         E  YW+I+NSWG  WGE
Sbjct:  244 VVPVNDEMSLKKAVAYQPISVMISAANMSDYKSGV---YKGACSNLWGDHNVLIVGYGTSS-----DEGDYWLIRNSWGPEWGE 319

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT1G47128.1 (| Symbols: RD21, RD21A | Granulin repeat cysteine protease family protein | chr1:17283139-17285609 REVERSE LENGTH=462)

HSP 1 Score: 52.373 bits (124), Expect = 3.043e-7
Identity = 34/95 (35.79%), Postives = 49/95 (51.58%), Query Frame = -3

Query:  356 VPVEKDQIGANLVKNGPFAVVINAAW--VQTFMSGV---SCPYICAKSRLDHGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEDITRSA 619
            VP   ++     V + P ++ I A     Q + SG+   SC      ++LDHGV+ VG+G  NG        K YWI++NSWG++WGE    R A
Sbjct:  251 VPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCG-----TQLDHGVVAVGYGTENG--------KDYWIVRNSWGKSWGESGYLRMA 332

[Close]

BLAST of GR399795 vs. TAIR peptide
Match: AT3G19390.1 (| Symbols: | Granulin repeat cysteine protease family protein | chr3:6723024-6724768 FORWARD LENGTH=452)

HSP 1 Score: 51.2174 bits (121), Expect = 6.779e-7
Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = -3

Query:  377 VPVEKDQIGANLVKNGPFAVVINAAW--VQTFMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGE 619
            VP   ++     + N P +V I A     Q + SGV     C  S LDHGV+ VG+G+         + YWI++NSWG NWGE
Sbjct:  244 VPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG-TCGTS-LDHGVVAVGYGSEGG------QDYWIVRNSWGSNWGE 318

The following BLAST results are available for this feature:

BLAST of GR399795 vs. SwissProt
BLAST of GR399795 vs. TrEMBL
BLAST of GR399795 vs. TAIR peptide

BLAST of GR399795 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CYSP_PEA	1.499e-37	82.56	Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1	[more]
CYSP1_MAIZE	4.744e-30	70.59	Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=...	[more]
A494_ARATH	1.503e-29	69.41	Probable cysteine proteinase A494 OS=Arabidopsis t...	[more]
RD19A_ARATH	9.741e-29	67.06	Cysteine proteinase RD19a OS=Arabidopsis thaliana ...	[more]
PAPA5_CARPA	1.114e-24	74.63	Cysteine proteinase (Fragment) OS=Carica papaya PE...	[more]
CPR1_DROME	1.783e-14	49.35	Putative cysteine proteinase CG12163 OS=Drosophila...	[more]
CYSP_TRYCR	1.278e-12	44.94	Cruzipain OS=Trypanosoma cruzi PE=1 SV=1	[more]
CATW_FELCA	2.333e-12	35.05	Cathepsin W OS=Felis catus GN=CTSW PE=2 SV=2	[more]
CYSP_TRYBB	3.719e-12	40.70	Cysteine proteinase OS=Trypanosoma brucei brucei P...	[more]
CATF_MOUSE	6.343e-12	43.75	Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1	[more]

BLAST of GR399795 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O24322_PHAVU	2.059e-40	69.05	Cysteine proteinase OS=Phaseolus vulgaris PE=3 SV=...	[more]
O81930_CICAR	1.284e-39	89.41	Cysteine proteinase OS=Cicer arietinum PE=2 SV=1	[more]
Q96455_SOYBN	1.479e-36	69.05	Thiol protease isoform A (Fragment) OS=Glycine max...	[more]
Q9STA4_MEDSA	5.048e-36	82.56	Cysteine protease (Fragment) OS=Medicago sativa GN...	[more]
Q8GV06_VICFA	1.918e-35	80.23	Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=...	[more]
Q41671_VICFA	1.918e-35	80.23	Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYS...	[more]
Q41698_VICSA	5.582e-35	79.07	Cysteine proteinase OS=Vicia sativa PE=2 SV=1	[more]
Q5ZF63_PLAMJ	4.890e-31	72.62	Cysteine protease 2 (Fragment) OS=Plantago major G...	[more]
B9RHA4_RICCO	1.089e-30	74.12	Cysteine protease, putative OS=Ricinus communis GN...	[more]
Q5Y804_PETHY	1.423e-30	72.62	Cysteine proteinase (Fragment) OS=Petunia hybrida ...	[more]

BLAST of GR399795 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT4G16190.1	3.946e-31	70.59	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT2G21430.1	1.500e-30	69.41	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT4G39090.1	9.720e-30	67.06	\| Symbols: RD19, RD19A \| Papain family cysteine pr...	[more]
AT3G54940.2	2.647e-27	58.62	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT3G43960.1	3.594e-8	41.67	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT1G47128.1	3.043e-7	35.79	\| Symbols: RD21, RD21A \| Granulin repeat cysteine ...	[more]
AT3G19390.1	6.779e-7	38.55	\| Symbols: \| Granulin repeat cysteine protease fa...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 82..92 score
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 35..119 score: 6.2
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 35..119 score: 3.1
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 35..119 score: 8.9
None	No IPR available	PANTHER	PTHR12411:SF42	CYSTEINE PROTEASE	coord: 35..119 score: 3.1
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 34..119 score: 1.7
None	No IPR available	SEG	seg	seg	coord: 22..35 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR399795 ID=GR399795; Name=GR399795; organism=Cicer arietinum; type=EST; length=732bp
ATGCTCCATAAACATTAGAAGATAATTAAGTTTACACTTATGATATTCTA
ATTCATGTACCTACCATGTTGCCATGGCTATGGATAAACAACACTTGAGT
AACAAGCATACATAATTACCAACTACATAAATAACATGATTGGGGTAGTT
TTCTGATCCTATATGGAGATAGTTTTGCCAGAACCAGAAGCTAAATTCAC
AACTTCATGATTATATATTTACACAAATTGAACTAGAGTCGTGTCACATA
GGATGAGACCACCAGAACCAGCATCTCCGTATTATTAATTGTTGGATGCA
TGAACTGCAGCTACAGTTGAGACCATTGAATCCACTCCACATATATTTCG
ACCTCTGCAGATCTTGTAATATCCTCCTCACCCCAATTCTGTCCCCATGA
ATTCTTTATGATCCAGTAAGGCTTTTCCTTCAATCGGATGGGAGCAAAAC
CATTTCCAAAACCAACTAGAAGAACCCCATGATCCAAACGCGATTTTGCA
CAAATGTATGGGCATGAAACACCACTCATAAATGTTTGCACCCAGGCTGC
GTTAATAACAACTGCAAAAGGGCCATTTTTTACTAAATTTGCACCAATTT
GGTCTTTCTCAACGGGAACAACCCTGAATTTTAAACGGAAACACCATTCT
TGTTTTTGTAAATTTACAGGAACCATCTCTTCTGGGTAAAAAAAACTTGT
TTNACAATAACCCCCCAAATGGGAAAGTATTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004197	cysteine-type endopeptidase activity
GO:0008234	cysteine-type peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A

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