GR401063

EST Overview
NameGR401063
Unique NameGR401063
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length330
Libraries
Library NameType
LIBEST_024783 ICC4958 Slow drought stresses root cDNA librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL2_ARATH (4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana GN=4CL2 PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 2.803e-13
Identity = 37/58 (63.79%), Postives = 44/58 (75.86%), Query Frame = -2 
Query:  153 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGFM 326
            VRS    I EDEIKQ++S+QVVFYKRI+KVFF +SI KA SGK LRK+L A+   G M
Sbjct:  498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 555
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.661e-13
Identity = 35/52 (67.31%), Postives = 44/52 (84.62%), Query Frame = -2 
Query:  171 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAK 326
            V+SNG  I EDEIKQ+IS+QV+FYKRI++VFF E+I KA SGK LRK+L A+
Sbjct:  493 VKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRAR 544
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.391e-12
Identity = 34/56 (60.71%), Postives = 44/56 (78.57%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VR+NGF   E+EIKQ++S+QVVFYKRI +VFF ++I K+ SGK LRK+L AK   G
Sbjct:  485 VRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.817e-12
Identity = 34/56 (60.71%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG  I EDE+K +IS+QV+FYKRI +VFF ++I K+ SGK LRK+L AK   G
Sbjct:  484 VRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 539
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.373e-12
Identity = 33/56 (58.93%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG  I EDE+K +IS+QV+FYKRI +VFF E++ K+ SGK LRK+L A+   G
Sbjct:  487 VRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.373e-12
Identity = 33/56 (58.93%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG  I EDE+K +IS+QV+FYKRI +VFF E++ K+ SGK LRK+L A+   G
Sbjct:  487 VRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL1_ARATH (4-coumarate--CoA ligase 1 OS=Arabidopsis thaliana GN=4CL1 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.373e-12
Identity = 34/56 (60.71%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            V+S    + ED++KQ++S+QVVFYKRI+KVFF ESI KA SGK LRK+L AK   G
Sbjct:  505 VKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANG 560
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.100e-12
Identity = 32/56 (57.14%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG  I EDE+K +IS+QV+FYKR+ +VFF E++ K+ SGK LRK+L A+   G
Sbjct:  489 VRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 544
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.100e-12
Identity = 33/56 (58.93%), Postives = 44/56 (78.57%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VR+NGF   E+EIKQ++S+QVVFYKRI +VFF ++I K+ SGK LRK+L A+   G
Sbjct:  485 VRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540
[Close]
BLAST of GR401063 vs. SwissProt
Match: 4CL4_ORYSJ (Probable 4-coumarate--CoA ligase 4 OS=Oryza sativa subsp. japonica GN=4CL4 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.538e-11
Identity = 33/56 (58.93%), Postives = 44/56 (78.57%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VR  G  I E+EIKQ+++++VVFYKR++KVFFA+SI K+ SGK LRK+L AK   G
Sbjct:  493 VRIEGSAISENEIKQFVAKEVVFYKRLNKVFFADSIPKSPSGKILRKDLRAKLAAG 548
[Close]
BLAST of GR401063 vs. TrEMBL
Match: Q8S564_SOYBN (4-coumarate:coenzyme A ligase OS=Glycine max GN=4CL1 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.289e-15
Identity = 42/59 (71.19%), Postives = 48/59 (81.36%), Query Frame = -2 
Query:  150 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGFMV 326
            VRSNG  I EDEIK+YISQQVVFYKRI +VFF +SI KA SGK LRK LTA+ N+G +V
Sbjct:  486 VRSNGSEIAEDEIKKYISQQVVFYKRICRVFFTDSIPKAPSGKILRKVLTARLNEGLVV 544
[Close]
BLAST of GR401063 vs. TrEMBL
Match: D7SQ89_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_52.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029182001 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.053e-13
Identity = 39/57 (68.42%), Postives = 46/57 (80.70%), Query Frame = -2 
Query:  156 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGF 326
            VRSNGF I EDEIK+YI++QVV+YKRI +VFF E+I KA SGK LRK+L AK   GF
Sbjct:  467 VRSNGFEITEDEIKEYIAKQVVYYKRIKRVFFIETIPKAPSGKILRKDLKAKLAAGF 523
[Close]
BLAST of GR401063 vs. TrEMBL
Match: O48869_9ROSI (4-coumarate:CoA ligase 1 OS=Populus trichocarpa x Populus deltoides GN=4CL1 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.063e-13
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VR+NG +I EDEIKQYIS+QVVFYKRIS+VFF E+I KA SGK LRK+L A+   G
Sbjct:  486 VRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLATG 541
[Close]
BLAST of GR401063 vs. TrEMBL
Match: O48868_9ROSI (4-coumarate:CoA ligase 2 OS=Populus trichocarpa x Populus deltoides GN=4CL2 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.063e-13
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG +I EDEIKQYIS+QV+FYKRI +VFF E+I KA SGK LRK+L A+ + G
Sbjct:  485 VRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRARVSAG 540
[Close]
BLAST of GR401063 vs. TrEMBL
Match: B9N2Y9_POPTR (4-Coumarate:CoA ligase OS=Populus trichocarpa GN=4CL1 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.063e-13
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VR+NG +I EDEIKQYIS+QVVFYKRIS+VFF E+I KA SGK LRK+L A+   G
Sbjct:  486 VRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLATG 541
[Close]
BLAST of GR401063 vs. TrEMBL
Match: Q9LL50_RUBID (4-coumarate:coA ligase 2 OS=Rubus idaeus PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 4.000e-13
Identity = 39/56 (69.64%), Postives = 45/56 (80.36%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG +I ED+IKQYIS+QVVFYKRISKVFF + I KA SGK LRK+L A+   G
Sbjct:  486 VRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLAAG 541
[Close]
BLAST of GR401063 vs. TrEMBL
Match: Q0GZS1_9BORA (4-coumarate:CoA ligase OS=Arnebia euchroma PE=2 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 4.000e-13
Identity = 37/58 (63.79%), Postives = 47/58 (81.03%), Query Frame = -2 
Query:  153 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGFM 326
            VRSNG  I EDEIKQ++S+QV+FYKRI++VFF +SI K+ SGK LRK+L AK   GF+
Sbjct:  487 VRSNGSTITEDEIKQFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLAAGFL 544
[Close]
BLAST of GR401063 vs. TrEMBL
Match: B9IM06_POPTR (4-Coumarate:CoA ligase (Fragment) OS=Populus trichocarpa GN=Ptr4CL1 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.823e-13
Identity = 38/56 (67.86%), Postives = 45/56 (80.36%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            VRSNG +I EDEIKQYIS+QV+FYKRI +VFF E+I KA SGK LRK+L A+   G
Sbjct:  485 VRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRARVAAG 540
[Close]
BLAST of GR401063 vs. TrEMBL
Match: B6RIV5_RUTGR (4-coumarate:CoA ligase 2 OS=Ruta graveolens PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.823e-13
Identity = 37/57 (64.91%), Postives = 47/57 (82.46%), Query Frame = -2 
Query:  156 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGF 326
            V+SNG +I EDEIKQ++S+QVVFYKRIS+VFF ++I KA SGK LRK+L AK   G+
Sbjct:  499 VKSNGSQISEDEIKQFVSKQVVFYKRISRVFFVDAIPKAPSGKILRKDLRAKLAAGY 555
[Close]
BLAST of GR401063 vs. TrEMBL
Match: B3TJI5_RUTGR (4-coumarate CoA ligase 2 OS=Ruta graveolens PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.823e-13
Identity = 37/57 (64.91%), Postives = 47/57 (82.46%), Query Frame = -2 
Query:  156 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGF 326
            V+SNG +I EDEIKQ++S+QVVFYKRIS+VFF ++I KA SGK LRK+L AK   G+
Sbjct:  499 VKSNGSQISEDEIKQFVSKQVVFYKRISRVFFVDAIPKAPSGKILRKDLRAKLAAGY 555
[Close]
BLAST of GR401063 vs. TAIR peptide
Match: AT3G21240.1 (| Symbols: 4CL2, AT4CL2 | 4-coumarate:CoA ligase 2 | chr3:7454497-7457314 REVERSE LENGTH=556)

HSP 1 Score: 73.9442 bits (180), Expect = 2.222e-14
Identity = 37/58 (63.79%), Postives = 44/58 (75.86%), Query Frame = -2 
Query:  153 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQGFM 326
            VRS    I EDEIKQ++S+QVVFYKRI+KVFF +SI KA SGK LRK+L A+   G M
Sbjct:  498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 555
[Close]
BLAST of GR401063 vs. TAIR peptide
Match: AT1G51680.1 (| Symbols: 4CL1, 4CL.1, AT4CL1 | 4-coumarate:CoA ligase 1 | chr1:19159007-19161464 REVERSE LENGTH=561)

HSP 1 Score: 70.8626 bits (172), Expect = 1.881e-13
Identity = 34/56 (60.71%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  159 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAKFNQG 326
            V+S    + ED++KQ++S+QVVFYKRI+KVFF ESI KA SGK LRK+L AK   G
Sbjct:  505 VKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANG 560
[Close]
BLAST of GR401063 vs. TAIR peptide
Match: AT1G65060.1 (| Symbols: 4CL3 | 4-coumarate:CoA ligase 3 | chr1:24167385-24171457 REVERSE LENGTH=561)

HSP 1 Score: 67.781 bits (164), Expect = 1.592e-12
Identity = 32/52 (61.54%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  171 VRSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAK 326
            VRSNG  I E+++K+Y+++QVVFYKR+ KVFF  SI K+ SGK LRK+L AK
Sbjct:  508 VRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAK 559
[Close]
BLAST of GR401063 vs. TAIR peptide
Match: AT3G21230.1 (| Symbols: 4CL5 | 4-coumarate:CoA ligase 5 | chr3:7448231-7451947 REVERSE LENGTH=570)

HSP 1 Score: 62.7734 bits (151), Expect = 5.123e-11
Identity = 30/51 (58.82%), Postives = 38/51 (74.51%), Query Frame = -2 
Query:  171 RSNGFRIPEDEIKQYISQQVVFYKRISKVFFAESILKAASGKFLRKELTAK 323
            RS G ++ ED++K Y+++QVV YKRI  VFF E I KA SGK LRK+L AK
Sbjct:  513 RSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLRAK 563
The following BLAST results are available for this feature:
BLAST of GR401063 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
4CL2_ARATH2.803e-1363.794-coumarate--CoA ligase 2 OS=Arabidopsis thaliana ... [more]
4CL_VANPL3.661e-1367.314-coumarate--CoA ligase OS=Vanilla planifolia GN=4... [more]
4CL2_PETCR1.391e-1260.714-coumarate--CoA ligase 1 OS=Petroselinum crispum ... [more]
4CL2_TOBAC1.817e-1260.714-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=... [more]
4CL2_SOLTU2.373e-1258.934-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=... [more]
4CL1_SOLTU2.373e-1258.934-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=... [more]
4CL1_ARATH2.373e-1260.714-coumarate--CoA ligase 1 OS=Arabidopsis thaliana ... [more]
4CL1_TOBAC3.100e-1257.144-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=... [more]
4CL1_PETCR3.100e-1258.934-coumarate--CoA ligase 1 OS=Petroselinum crispum ... [more]
4CL4_ORYSJ1.538e-1158.93Probable 4-coumarate--CoA ligase 4 OS=Oryza sativa... [more]
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BLAST of GR401063 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q8S564_SOYBN7.289e-1571.194-coumarate:coenzyme A ligase OS=Glycine max GN=4C... [more]
D7SQ89_VITVI1.053e-1368.42Whole genome shotgun sequence of line PN40024, sca... [more]
O48869_9ROSI3.063e-1369.644-coumarate:CoA ligase 1 OS=Populus trichocarpa x ... [more]
O48868_9ROSI3.063e-1367.864-coumarate:CoA ligase 2 OS=Populus trichocarpa x ... [more]
B9N2Y9_POPTR3.063e-1369.644-Coumarate:CoA ligase OS=Populus trichocarpa GN=4... [more]
Q9LL50_RUBID4.000e-1369.644-coumarate:coA ligase 2 OS=Rubus idaeus PE=2 SV=1[more]
Q0GZS1_9BORA4.000e-1363.794-coumarate:CoA ligase OS=Arnebia euchroma PE=2 SV... [more]
B9IM06_POPTR6.823e-1367.864-Coumarate:CoA ligase (Fragment) OS=Populus trich... [more]
B6RIV5_RUTGR6.823e-1364.914-coumarate:CoA ligase 2 OS=Ruta graveolens PE=4 S... [more]
B3TJI5_RUTGR6.823e-1364.914-coumarate CoA ligase 2 OS=Ruta graveolens PE=2 S... [more]
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BLAST of GR401063 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4
Match NameE-valueIdentityDescription
AT3G21240.12.222e-1463.79| Symbols: 4CL2, AT4CL2 | 4-coumarate:CoA ligase 2... [more]
AT1G51680.11.881e-1360.71| Symbols: 4CL1, 4CL.1, AT4CL1 | 4-coumarate:CoA l... [more]
AT1G65060.11.592e-1261.54| Symbols: 4CL3 | 4-coumarate:CoA ligase 3 | chr1:... [more]
AT3G21230.15.123e-1158.82| Symbols: 4CL5 | 4-coumarate:CoA ligase 5 | chr3:... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30no descriptioncoord: 4..55
score: 2.9
NoneNo IPR availablePANTHERPTHR11968ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBERcoord: 2..56
score: 5.1
NoneNo IPR availablePANTHERPTHR11968:SF434-COUMARATE--COA LIGASEcoord: 2..56
score: 5.1
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 2..57
score: 1

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR401063 ID=GR401063; Name=GR401063; organism=Cicer arietinum; type=EST; length=330bp
GCGCAAGAACAAAATCATAAAATACTTGTATTGCATGCATGTTGATAATG
AGCTAAAAAAAAGGAACAAAAACCAAAATAGGAAGGGCCTGTGATGAGGT
ATGAAAAGCAACCAAAAATTTCATCTGGGACATGGACCTAATCGGCCATC
ACCATAAAACCTTGGTTAAATTTTGCTGTTAATTCCTTCCGCAAAAATTT
GCCTGAAGCTGCTTTAAGAATAGACTCCGCGAAGAAAACTTTACTGATTC
TTTTGTAAAATACCACCTGTTGTGAAATGTATTGCTTGATTTCATCCTCG
GGGATCCTAAAACCATTTGATCTTACCCGC
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