GR408671

EST Overview


Name	GR408671
Unique Name	GR408671
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	526

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E2_WHEAT (Eukaryotic translation initiation factor 4E-2 OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 6.992e-31
Identity = 63/93 (67.74%), Postives = 78/93 (83.87%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNR--GAKARYTV 521
            SRK  LD MWLET MALIGEQF +S++ICGVVASVRQ QDKLSLWTKTA+NE+VQ+ IG+KWKE+I+ NDKM Y+FH+D++++      RYTV
Sbjct:  117 SRKTNLDTMWLETCMALIGEQFDESQEICGVVASVRQRQDKLSLWTKTASNEAVQVDIGKKWKEVIDYNDKMVYSFHDDSRSQKPSRGGRYTV 209

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E2_ORYSJ (Eukaryotic translation initiation factor 4E-2 OS=Oryza sativa subsp. japonica GN=Os10g0467600 PE=2 SV=2)

HSP 1 Score: 130.954 bits (328), Expect = 3.470e-30
Identity = 60/81 (74.07%), Postives = 74/81 (91.36%), Query Frame = -3

Query:  279 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK 521
            SRK TL+ MWLETLMALIGEQF +SE+ICGVVASVRQ  DKL+LWT+TA+NE+VQ++IG+KWKEI++ NDKM Y+FH+DAK
Sbjct:  114 SRKTTLENMWLETLMALIGEQFDESEEICGVVASVRQRGDKLALWTRTASNEAVQVNIGKKWKEIVDYNDKMVYSFHDDAK 194

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E2_MAIZE (Eukaryotic translation initiation factor 4E-2 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 8.547e-29
Identity = 60/95 (63.16%), Postives = 79/95 (83.16%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK----NRGAKARYTV 521
            +RK T + MWLETLMALIGEQF ++EDICG+VASVR   DKL+LWT+TA+NE+VQ++IG+KWK++I+ NDK+TY FH+D+K    +RG   RYTV
Sbjct:  124 NRKATFETMWLETLMALIGEQFDETEDICGIVASVRARGDKLALWTRTASNEAVQVNIGKKWKDVIDYNDKITYTFHDDSKRDKPSRG--GRYTV 216

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E2_ARATH (Eukaryotic translation initiation factor 4E-2 OS=Arabidopsis thaliana GN=EIF4E2 PE=1 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 1.668e-24
Identity = 55/93 (59.14%), Postives = 74/93 (79.57%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQW--QDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGAKARYTV 521
            +RK  LD  WLETLMALIGEQF ++++ICGVVASVR    QDKLSLWT+T +NE+V M IG+KWKEI++  DK+T+N H+D++    ++R+TV
Sbjct:  110 NRKEALDKGWLETLMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHDDSR----RSRFTV 198

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E5_ARATH (Eukaryotic translation initiation factor 4E-5 OS=Arabidopsis thaliana GN=At1g29590 PE=2 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 1.322e-21
Identity = 48/91 (52.75%), Postives = 66/91 (72.53%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 515
            + TL++ WL TL+AL+GEQF   ++ICG V + R   D++SLWTK AANE  Q+SIG++WKE++  ND + +  HEDAK  +R AK RYTV
Sbjct:  150 RATLESNWLNTLLALVGEQFDQGDEICGAVLNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYTV 240

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E4_ARATH (Putative eukaryotic translation initiation factor 4E-4 OS=Arabidopsis thaliana GN=At1g29550 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.726e-21
Identity = 48/91 (52.75%), Postives = 65/91 (71.43%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 515
            K TL+  WL TL+AL+GEQF   ++ICG V + R   D++SLWTK AANE  Q+SIG++WKE++  N+ + +  HEDAK  +R AK RYTV
Sbjct:  150 KATLECNWLNTLLALVGEQFDQGDEICGAVLNFRARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDAKRRYTV 240

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E1_WHEAT (Eukaryotic translation initiation factor 4E-1 OS=Triticum aestivum PE=1 SV=3)

HSP 1 Score: 102.064 bits (253), Expect = 1.726e-21
Identity = 46/87 (52.87%), Postives = 65/87 (74.71%), Query Frame = -3

Query:  249 DAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 503
            D  WL TL+A+IGEQF   ++ICG V SVRQ Q+++++WTK AANE+ Q+SIG++WKE ++  D + +  HEDAK  ++G K RYTV
Sbjct:  129 DTFWLHTLLAMIGEQFDFGDEICGAVVSVRQKQERVAIWTKNAANEAAQISIGKQWKEFLDYKDSIGFIVHEDAKRSDKGPKNRYTV 215

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E1_ORYSJ (Eukaryotic translation initiation factor 4E-1 OS=Oryza sativa subsp. japonica GN=Os01g0970400 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.022e-21
Identity = 45/87 (51.72%), Postives = 65/87 (74.71%), Query Frame = -3

Query:  249 DAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 503
            D MWL TL+A+IGEQF   ++ICG V SVR  Q+++++WTK AANE+ Q+SIG++WKE ++  D + +  H+DAK  ++G K RYTV
Sbjct:  141 DTMWLHTLLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQISIGKQWKEFLDYKDSIGFIVHDDAKKMDKGLKNRYTV 227

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E1_MAIZE (Eukaryotic translation initiation factor 4E-1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 8.567e-21
Identity = 44/87 (50.57%), Postives = 65/87 (74.71%), Query Frame = -3

Query:  249 DAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 503
            D  WL TL+A+IGEQF   ++ICG V SVR  Q+++++WTK AANE+ Q+SIG++WKE+++  D + +  H+DAK  ++G K RYTV
Sbjct:  132 DTFWLHTLLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQVSIGKQWKELLDYKDSIGFIVHDDAKKMDKGLKERYTV 218

[Close]

BLAST of GR408671 vs. SwissProt
Match: IF4E1_ARATH (Eukaryotic translation initiation factor 4E-1 OS=Arabidopsis thaliana GN=EIF4E1 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 9.472e-20
Identity = 44/90 (48.89%), Postives = 64/90 (71.11%), Query Frame = -3

Query:  252 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYT 515
            K   D  WL TL+ALIGEQF   ++ICG V ++R  Q+++S+WTK A+NE+ Q+SIG++WKE ++ N+ + +  HEDAK  +R AK  YT
Sbjct:  145 KEKSDKSWLYTLLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAKKLDRNAKNAYT 234

[Close]

BLAST of GR408671 vs. TrEMBL
Match: A0EXV1_PEA (Eukaryotic translation initiation factor 4E iso OS=Pisum sativum GN=eIF4Eiso PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 5.119e-41
Identity = 82/89 (92.13%), Postives = 84/89 (94.38%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGAKARYTV 515
            K  LD MWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEII+ +DKMTYNFHEDAK RGAKARYTV
Sbjct:  116 KGNLDTMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIDVSDKMTYNFHEDAKTRGAKARYTV 204

[Close]

BLAST of GR408671 vs. TrEMBL
Match: D3TI64_PHAVU (Eukaryotic translation initiation factor iso4E OS=Phaseolus vulgaris GN=eIFiso4E PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 9.990e-37
Identity = 75/93 (80.65%), Postives = 83/93 (89.25%), Query Frame = -3

Query:  249 ISRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGA-KARYTV 524
            I RK  LD MWLETLMALIGEQF D+EDICGVVASVRQWQDKLSLWTKTAANE+ QMSIGRKWKEII+ NDK+TYNFH+D++ +GA K RYTV
Sbjct:  107 IGRKANLDNMWLETLMALIGEQFEDAEDICGVVASVRQWQDKLSLWTKTAANEAAQMSIGRKWKEIIDVNDKITYNFHDDSRTKGATKGRYTV 199

[Close]

BLAST of GR408671 vs. TrEMBL
Match: C6TKG0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 4.958e-36
Identity = 73/91 (80.22%), Postives = 82/91 (90.11%), Query Frame = -3

Query:  249 RKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGA-KARYTV 518
            RK  LD MWLET+MALIGEQF D+EDICGVVASVRQWQDKLSLWTKTAANE+ QMSIGRKWKEII+ NDK+TYNFH+D++ +GA K RYTV
Sbjct:  110 RKANLDNMWLETMMALIGEQFEDAEDICGVVASVRQWQDKLSLWTKTAANEAAQMSIGRKWKEIIDVNDKITYNFHDDSRTKGASKGRYTV 200

[Close]

BLAST of GR408671 vs. TrEMBL
Match: D3TI58_PHAVU (Eukaryotic translation initiation factor iso4E (Fragment) OS=Phaseolus vulgaris GN=eIFiso4E PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 4.197e-35
Identity = 70/86 (81.40%), Postives = 78/86 (90.70%), Query Frame = -3

Query:  267 ISRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGA 524
            I RK  LD MWLETLMALIGEQF D+EDICGVVASVRQWQDKLSLWTKTAANE+ QMSIGRKWKEII+ NDK+TYNFH+D++ +GA
Sbjct:  100 IGRKANLDNMWLETLMALIGEQFEDAEDICGVVASVRQWQDKLSLWTKTAANEAAQMSIGRKWKEIIDVNDKITYNFHDDSRTKGA 185

[Close]

BLAST of GR408671 vs. TrEMBL
Match: Q7XJB0_LACSA (Eukaryotic translation initiation factor iso4E OS=Lactuca sativa GN=eIF(iso)4E PE=2 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 2.546e-32
Identity = 66/91 (72.53%), Postives = 78/91 (85.71%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGAKARYTV 521
            SRK  L+ MW ETLMALIGEQF ++++ICGVVASVRQ QDKLSLWTK AANE+ QMSIGRKWKEII+  DK+TYNFH+D+K R +K RY+V
Sbjct:  103 SRKANLETMWFETLMALIGEQFDEADEICGVVASVRQRQDKLSLWTKNAANEAAQMSIGRKWKEIIDVTDKITYNFHDDSKTRTSKGRYSV 193

[Close]

BLAST of GR408671 vs. TrEMBL
Match: B9HKU1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_720440 PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 2.546e-32
Identity = 69/92 (75.00%), Postives = 79/92 (85.87%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK-NRGAKARYTV 521
            SRK  LD MWLETLMALIGEQF + +DICGVVASVRQ QDKL+LWTK AANE+ QMSIGRKWKEII+ NDK+TY+FH+D+K  R AK+RY V
Sbjct:  116 SRKADLDTMWLETLMALIGEQFDEPDDICGVVASVRQRQDKLALWTKAAANEAAQMSIGRKWKEIIDVNDKITYSFHDDSKRERNAKSRYNV 207

[Close]

BLAST of GR408671 vs. TrEMBL
Match: B0F816_CUCME (Eukaryotic initiation factor iso4E OS=Cucumis melo GN=eIF(iso)4E PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.815e-31
Identity = 67/92 (72.83%), Postives = 80/92 (86.96%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHED-AKNRGAKARYTV 521
            SRK  LD MWLETLMALIGEQF +S++ICGVVASVRQ QDKL+LWTKTA NE+ QMSIGRKWKEII+ NDK++++FHED  + + AKARY+V
Sbjct:  112 SRKANLDNMWLETLMALIGEQFEESDEICGVVASVRQRQDKLALWTKTATNEAAQMSIGRKWKEIIDVNDKISFSFHEDLRREKSAKARYSV 203

[Close]

BLAST of GR408671 vs. TrEMBL
Match: Q66WU1_TOBAC (Eukaryotic initiation factor iso4E OS=Nicotiana tabacum GN=eIFiso4E PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.070e-30
Identity = 66/92 (71.74%), Postives = 83/92 (90.22%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK-NRGAKARYTV 521
            SRK  L+ MWLETLMAL+GEQF +SE+ICGVVASVR+ QDKLSLWTKTA+NE++QMSIGRKWKEII++ +K++Y+FH+D+K  R AK+RYTV
Sbjct:  110 SRKANLETMWLETLMALVGEQFDESEEICGVVASVRRSQDKLSLWTKTASNEAIQMSIGRKWKEIIDA-EKISYSFHDDSKRERSAKSRYTV 200

[Close]

BLAST of GR408671 vs. TrEMBL
Match: Q4PZB3_CAPAN (Eukaryotic initiation factor 4E OS=Capsicum annuum GN=eIF(iso)4E PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.070e-30
Identity = 67/92 (72.83%), Postives = 80/92 (86.96%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK-NRGAKARYTV 521
            SRK  L+ MWLETLMAL+GEQF DSEDICGVVASVR+ QDKLSLWTKTA NE+ QM IGRKWKEII++ +K++Y+FH+D+K  R AK+RYTV
Sbjct:  112 SRKANLETMWLETLMALVGEQFDDSEDICGVVASVRRSQDKLSLWTKTATNEAAQMGIGRKWKEIIDT-EKISYSFHDDSKRERSAKSRYTV 202

[Close]

BLAST of GR408671 vs. TrEMBL
Match: D3UW25_TOBAC (Translation initiation factor eIF(Iso)4E OS=Nicotiana tabacum GN=eIF(iso)4E PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.070e-30
Identity = 66/92 (71.74%), Postives = 83/92 (90.22%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK-NRGAKARYTV 521
            SRK  L+ MWLETLMAL+GEQF +SE+ICGVVASVR+ QDKLSLWTKTA+NE++QMSIGRKWKEII++ +K++Y+FH+D+K  R AK+RYTV
Sbjct:  105 SRKANLETMWLETLMALVGEQFDESEEICGVVASVRRSQDKLSLWTKTASNEAIQMSIGRKWKEIIDA-EKISYSFHDDSKRERSAKSRYTV 195

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT5G35620.1 (| Symbols: LSP1, EIF(ISO)4E, LSP, EIF4E2, eIFiso4E | Eukaryotic initiation factor 4E protein | chr5:13824946-13826331 REVERSE LENGTH=198)

HSP 1 Score: 112.079 bits (279), Expect = 1.837e-25
Identity = 55/93 (59.14%), Postives = 74/93 (79.57%), Query Frame = -3

Query:  249 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQW--QDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAKNRGAKARYTV 521
            +RK  LD  WLETLMALIGEQF ++++ICGVVASVR    QDKLSLWT+T +NE+V M IG+KWKEI++  DK+T+N H+D++    ++R+TV
Sbjct:  110 NRKEALDKGWLETLMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHDDSR----RSRFTV 198

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT1G29590.1 (| Symbols: eIF4E3 | Eukaryotic initiation factor 4E protein | chr1:10339937-10341628 FORWARD LENGTH=285)

HSP 1 Score: 102.449 bits (254), Expect = 1.455e-22
Identity = 48/91 (52.75%), Postives = 66/91 (72.53%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 515
            + TL++ WL TL+AL+GEQF   ++ICG V + R   D++SLWTK AANE  Q+SIG++WKE++  ND + +  HEDAK  +R AK RYTV
Sbjct:  195 RATLESNWLNTLLALVGEQFDQGDEICGAVLNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYTV 285

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT1G29590.2 (| Symbols: eIF4E3 | Eukaryotic initiation factor 4E protein | chr1:10340072-10342750 FORWARD LENGTH=300)

HSP 1 Score: 102.064 bits (253), Expect = 1.901e-22
Identity = 47/91 (51.65%), Postives = 66/91 (72.53%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 515
            + TL++ WL TL+AL+GEQF   ++ICG V + R   D++SLWTK AANE  Q+SIG++WKE++  ND + +  HEDAK  +R AK RYT+
Sbjct:  150 RATLESNWLNTLLALVGEQFDQGDEICGAVLNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYTI 240

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT1G29550.1 (| Symbols: | Eukaryotic initiation factor 4E protein | chr1:10330514-10332094 FORWARD LENGTH=240)

HSP 1 Score: 102.064 bits (253), Expect = 1.901e-22
Identity = 48/91 (52.75%), Postives = 65/91 (71.43%), Query Frame = -3

Query:  249 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYTV 515
            K TL+  WL TL+AL+GEQF   ++ICG V + R   D++SLWTK AANE  Q+SIG++WKE++  N+ + +  HEDAK  +R AK RYTV
Sbjct:  150 KATLECNWLNTLLALVGEQFDQGDEICGAVLNFRARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDAKRRYTV 240

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT4G18040.1 (| Symbols: EIF4E, CUM1, AT.EIF4E1, eIF4E1 | eukaryotic translation initiation factor 4E | chr4:10016724-10018151 REVERSE LENGTH=235)

HSP 1 Score: 96.2857 bits (238), Expect = 1.043e-20
Identity = 44/90 (48.89%), Postives = 64/90 (71.11%), Query Frame = -3

Query:  252 KPTLDAMWLETLMALIGEQFGDSEDICGVVASVRQWQDKLSLWTKTAANESVQMSIGRKWKEIIESNDKMTYNFHEDAK--NRGAKARYT 515
            K   D  WL TL+ALIGEQF   ++ICG V ++R  Q+++S+WTK A+NE+ Q+SIG++WKE ++ N+ + +  HEDAK  +R AK  YT
Sbjct:  145 KEKSDKSWLYTLLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAKKLDRNAKNAYT 234

[Close]

BLAST of GR408671 vs. TAIR peptide
Match: AT5G35620.2 (| Symbols: LSP1, EIF(ISO)4E, LSP, EIF4E2, eIFiso4E | Eukaryotic initiation factor 4E protein | chr5:13825570-13826331 REVERSE LENGTH=167)

HSP 1 Score: 75.8702 bits (185), Expect = 1.459e-14
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = -3

Query:  360 SRKPTLDAMWLETLMALIGEQFGDSEDICGVVASVR--QWQDKLSLWTKTAANESV 521
            +RK  LD  WLETLMALIGEQF ++++ICGVVASVR    QDKLSLWT+T +NE+V
Sbjct:  110 NRKEALDKGWLETLMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAV 165

The following BLAST results are available for this feature:

BLAST of GR408671 vs. SwissProt
BLAST of GR408671 vs. TrEMBL
BLAST of GR408671 vs. TAIR peptide

BLAST of GR408671 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
IF4E2_WHEAT	6.992e-31	67.74	Eukaryotic translation initiation factor 4E-2 OS=T...	[more]
IF4E2_ORYSJ	3.470e-30	74.07	Eukaryotic translation initiation factor 4E-2 OS=O...	[more]
IF4E2_MAIZE	8.547e-29	63.16	Eukaryotic translation initiation factor 4E-2 OS=Z...	[more]
IF4E2_ARATH	1.668e-24	59.14	Eukaryotic translation initiation factor 4E-2 OS=A...	[more]
IF4E5_ARATH	1.322e-21	52.75	Eukaryotic translation initiation factor 4E-5 OS=A...	[more]
IF4E4_ARATH	1.726e-21	52.75	Putative eukaryotic translation initiation factor ...	[more]
IF4E1_WHEAT	1.726e-21	52.87	Eukaryotic translation initiation factor 4E-1 OS=T...	[more]
IF4E1_ORYSJ	5.022e-21	51.72	Eukaryotic translation initiation factor 4E-1 OS=O...	[more]
IF4E1_MAIZE	8.567e-21	50.57	Eukaryotic translation initiation factor 4E-1 OS=Z...	[more]
IF4E1_ARATH	9.472e-20	48.89	Eukaryotic translation initiation factor 4E-1 OS=A...	[more]

BLAST of GR408671 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A0EXV1_PEA	5.119e-41	92.13	Eukaryotic translation initiation factor 4E iso OS...	[more]
D3TI64_PHAVU	9.990e-37	80.65	Eukaryotic translation initiation factor iso4E OS=...	[more]
C6TKG0_SOYBN	4.958e-36	80.22	Putative uncharacterized protein OS=Glycine max PE...	[more]
D3TI58_PHAVU	4.197e-35	81.40	Eukaryotic translation initiation factor iso4E (Fr...	[more]
Q7XJB0_LACSA	2.546e-32	72.53	Eukaryotic translation initiation factor iso4E OS=...	[more]
B9HKU1_POPTR	2.546e-32	75.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B0F816_CUCME	2.815e-31	72.83	Eukaryotic initiation factor iso4E OS=Cucumis melo...	[more]
Q66WU1_TOBAC	1.070e-30	71.74	Eukaryotic initiation factor iso4E OS=Nicotiana ta...	[more]
Q4PZB3_CAPAN	1.070e-30	72.83	Eukaryotic initiation factor 4E OS=Capsicum annuum...	[more]
D3UW25_TOBAC	1.070e-30	71.74	Translation initiation factor eIF(Iso)4E OS=Nicoti...	[more]

BLAST of GR408671 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G35620.1	1.837e-25	59.14	\| Symbols: LSP1, EIF(ISO)4E, LSP, EIF4E2, eIFiso4E...	[more]
AT1G29590.1	1.455e-22	52.75	\| Symbols: eIF4E3 \| Eukaryotic initiation factor 4...	[more]
AT1G29590.2	1.901e-22	51.65	\| Symbols: eIF4E3 \| Eukaryotic initiation factor 4...	[more]
AT1G29550.1	1.901e-22	52.75	\| Symbols: \| Eukaryotic initiation factor 4E prot...	[more]
AT4G18040.1	1.043e-20	48.89	\| Symbols: EIF4E, CUM1, AT.EIF4E1, eIF4E1 \| eukary...	[more]
AT5G35620.2	1.459e-14	67.86	\| Symbols: LSP1, EIF(ISO)4E, LSP, EIF4E2, eIFiso4E...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001040	Eukaryotic translation initiation factor 4E (eIF-4E)	GENE3D	3.30.760.10	no description	coord: 4..87 score: 4.1
IPR001040	Eukaryotic translation initiation factor 4E (eIF-4E)	PANTHER	PTHR11960	EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED	coord: 1..85 score: 1.2
IPR001040	Eukaryotic translation initiation factor 4E (eIF-4E)	PFAM	PF01652	IF4E	coord: 4..76 score: 1.1
IPR001040	Eukaryotic translation initiation factor 4E (eIF-4E)	SUPERFAMILY	55418	eIF4e-like	coord: 4..92 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408671 ID=GR408671; Name=GR408671; organism=Cicer arietinum; type=EST; length=526bp
AGTAAAAATATTCAAAATTTCATGCTTGTATAATGTTACATGTGAAAACA
AAAGTATAATGAAAAATGACAGGACAATTTCTCAAGAATTGGAACAAAGT
ATAATTAATAGTATTACACCAAAATATTGATAGGGGAAAAAAAAATTAAC
CCCCCAACCAAAACAAAAGTGCATTCTAATACACATAGCATAAATCAATA
CAACAGCATTGGCAGCACAAGCGTGCGAAAATGTTCTGGTAGTATTTATA
CAGTGTATCGAGCCTTTGCACCTCGATTTTTAGCATCTTCATGAAAGTTA
TATGTCATCTTGTCGTTAGATTCGATGATTTCCTTCCACTTTCTTCCAAT
GCTCATCTGAACAGACTCATTAGCTGCCGTTTTTGTCCACAATGAAAGTT
TGTCCTGCCACTGGCGCACGCTCGCAACAACACCACAAATGTCCTCGGAG
TCTCCAAATTGCTCCCCAATTAAAGCCATCAAAGTTTCTAGCCACATGGC
ATCAAGAGTGGGCTTTCTACTGATGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0003743	translation initiation factor activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006413	translational initiation

Vocabulary: **INTERPRO**
Term	Definition
IPR001040	TIF_eIF_4E

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