GR421065

EST Overview


Name	GR421065
Unique Name	GR421065
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	532

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR421065 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 2.890e-11
Identity = 31/40 (77.50%), Postives = 33/40 (82.50%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRR CPGE LA+R ISMTLALLIQCFDWK  + D I
Sbjct:  428 AFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKI 467

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.934e-15
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI
Sbjct:  428 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 467

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.902e-14
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRRVCPGEALALRTISMTLALL+QCFDWKRTSDD I
Sbjct:  428 AFGMGRRVCPGEALALRTISMTLALLVQCFDWKRTSDDKI 467

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.968e-11
Identity = 32/40 (80.00%), Postives = 35/40 (87.50%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRR CPGE LALR ISMTLALL+QCFDWKR +D+ I
Sbjct:  428 AFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKI 467

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 7.478e-11
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRR CPGE LA+R ISMTLALLIQCFDWK T+ D I
Sbjct:  428 AFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLTNGDKI 467

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 9.766e-11
Identity = 31/39 (79.49%), Postives = 34/39 (87.18%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPGE LA+R ISMTLALLIQCFDWKR SD+ I
Sbjct:  430 FGLGRRACPGELLAIRAISMTLALLIQCFDWKRVSDEEI 468

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.142e-9
Identity = 26/40 (65.00%), Postives = 34/40 (85.00%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFGMGRR CPGE +A++++S TL LLIQCFDWKR S++ +
Sbjct:  432 AFGMGRRACPGEPMAMQSVSFTLGLLIQCFDWKRVSEEKL 471

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q8L6V5_CICAR (Putative cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.194e-8
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = -2

Query:  274 GEALALRTISMTLALLIQCFDWKRTSDDMI 363
            GEALALRTISMTLALLIQCFDWKRTSDDMI
Sbjct:    3 GEALALRTISMTLALLIQCFDWKRTSDDMI 32

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q2LAL0_SOYBN (Cytochrome P450 monooxygenase CYP81E11 OS=Glycine max GN=CYP81E11 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.559e-8
Identity = 25/37 (67.57%), Postives = 32/37 (86.49%), Query Frame = -2

Query:  283 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSD 393
            AFGMGRR CPGE +A++++S TL L+IQCFDWKR S+
Sbjct:  432 AFGMGRRACPGEPMAMQSVSYTLGLMIQCFDWKRVSE 468

[Close]

BLAST of GR421065 vs. TrEMBL
Match: B7FM85_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.559e-8
Identity = 27/39 (69.23%), Postives = 32/39 (82.05%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPG  L  RT+S+TLALLIQCFDWKR S++ I
Sbjct:  433 FGLGRRACPGANLGQRTVSLTLALLIQCFDWKRISEEEI 471

[Close]

BLAST of GR421065 vs. TrEMBL
Match: Q9SML1_CICAR (Cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum GN=cyp81E4 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.036e-8
Identity = 25/40 (62.50%), Postives = 34/40 (85.00%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFG+GRR CPG++LA RT+ +TL LLIQCF+WKR +++ I
Sbjct:  137 AFGLGRRACPGQSLAHRTVGLTLGLLIQCFEWKRETEEKI 176

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 53.9138 bits (128), Expect = 6.063e-8
Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            +FGMGRR CPG  LA R I+  L  L+QCF+W+R  +D +
Sbjct:  423 SFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFV 462

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT2G23220.1 (| Symbols: CYP81D6 | cytochrome P450, family 81, subfamily D, polypeptide 6 | chr2:9884550-9886752 FORWARD LENGTH=515)

HSP 1 Score: 53.5286 bits (127), Expect = 7.919e-8
Identity = 20/40 (50.00%), Postives = 29/40 (72.50%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            +FGMGRR CPG  LA R +S+TL  ++QCF+W+R   + +
Sbjct:  441 SFGMGRRACPGYGLAHRVVSLTLGSMVQCFEWQRIGQEYV 480

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327)

HSP 1 Score: 52.7582 bits (125), Expect = 1.351e-7
Identity = 20/39 (51.28%), Postives = 29/39 (74.36%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +
Sbjct:  255 FGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEV 293

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 52.7582 bits (125), Expect = 1.351e-7
Identity = 20/39 (51.28%), Postives = 29/39 (74.36%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +
Sbjct:  314 FGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEV 352

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT4G37410.1 (| Symbols: CYP81F4 | cytochrome P450, family 81, subfamily F, polypeptide 4 | chr4:17590848-17592780 FORWARD LENGTH=501)

HSP 1 Score: 52.373 bits (124), Expect = 1.764e-7
Identity = 21/40 (52.50%), Postives = 29/40 (72.50%), Query Frame = -2

Query:  274 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 393
            AFG GRR+CPG  LA + +++ L  LIQCFDWK+ ++  I
Sbjct:  427 AFGSGRRICPGVGLAHKIVTLALGSLIQCFDWKKVNEKEI 466

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 51.9878 bits (123), Expect = 2.304e-7
Identity = 20/39 (51.28%), Postives = 28/39 (71.79%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPG  LA R  S+T+  LIQCF+W+R  ++ +
Sbjct:  427 FGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEV 465

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 51.6026 bits (122), Expect = 3.009e-7
Identity = 19/39 (48.72%), Postives = 29/39 (74.36%), Query Frame = -2

Query:  274 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMI 390
            FG+GRR CPG  LA R ++++L  LIQCF+W+R  ++ +
Sbjct:  427 FGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEV 465

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 51.2174 bits (121), Expect = 3.930e-7
Identity = 20/37 (54.05%), Postives = 26/37 (70.27%), Query Frame = -2

Query:  283 AFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSD 393
            AFG+GRR CPG  LA R + + L  LIQCF+W+R  +
Sbjct:  432 AFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGN 468

[Close]

BLAST of GR421065 vs. TAIR peptide
Match: AT5G67310.1 (| Symbols: CYP81G1 | cytochrome P450, family 81, subfamily G, polypeptide 1 | chr5:26855313-26856924 REVERSE LENGTH=507)

HSP 1 Score: 50.8322 bits (120), Expect = 5.133e-7
Identity = 21/36 (58.33%), Postives = 26/36 (72.22%), Query Frame = -2

Query:  283 FGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSD 390
            FG+GRR CPG  LA   +S+ L  LIQCFDW+R +D
Sbjct:  438 FGIGRRACPGMGLAQLVLSLALGSLIQCFDWERDND 473

The following BLAST results are available for this feature:

BLAST of GR421065 vs. SwissProt
BLAST of GR421065 vs. TrEMBL
BLAST of GR421065 vs. TAIR peptide

BLAST of GR421065 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
C81E1_GLYEC	2.890e-11	77.50	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ...	[more]

BLAST of GR421065 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9XFX0_CICAR	2.934e-15	100.00	Cytochrome P450 monooxygenase OS=Cicer arietinum G...	[more]
Q9ZRW6_CICAR	1.902e-14	95.00	Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2...	[more]
Q6WNR0_MEDTR	1.968e-11	80.00	Isoflavone 2'-hydroxylase OS=Medicago truncatula P...	[more]
Q9ZWF2_GLYEC	7.478e-11	80.00	Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-...	[more]
Q9MBE4_LOTJA	9.766e-11	79.49	Cytochrome P450 OS=Lotus japonicus PE=2 SV=1	[more]
Q2LAK9_SOYBN	3.142e-9	65.00	Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ...	[more]
Q8L6V5_CICAR	1.194e-8	100.00	Putative cytochrome P450 monooxygenase (Fragment) ...	[more]
Q2LAL0_SOYBN	1.559e-8	67.57	Cytochrome P450 monooxygenase CYP81E11 OS=Glycine ...	[more]
B7FM85_MEDTR	1.559e-8	69.23	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q9SML1_CICAR	2.036e-8	62.50	Cytochrome P450 monooxygenase (Fragment) OS=Cicer ...	[more]

BLAST of GR421065 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT4G37330.1	6.063e-8	52.50	\| Symbols: CYP81D4 \| cytochrome P450, family 81, s...	[more]
AT2G23220.1	7.919e-8	50.00	\| Symbols: CYP81D6 \| cytochrome P450, family 81, s...	[more]
AT1G66540.2	1.351e-7	51.28	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT1G66540.1	1.351e-7	51.28	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT4G37410.1	1.764e-7	52.50	\| Symbols: CYP81F4 \| cytochrome P450, family 81, s...	[more]
AT4G37340.1	2.304e-7	51.28	\| Symbols: CYP81D3 \| cytochrome P450, family 81, s...	[more]
AT4G37360.1	3.009e-7	48.72	\| Symbols: CYP81D2 \| cytochrome P450, family 81, s...	[more]
AT5G36220.1	3.930e-7	54.05	\| Symbols: CYP81D1, CYP91A1 \| cytochrome p450 81d1...	[more]
AT5G67310.1	5.133e-7	58.33	\| Symbols: CYP81G1 \| cytochrome P450, family 81, s...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 8..20 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR421065 ID=GR421065; Name=GR421065; organism=Cicer arietinum; type=EST; length=532bp
ATTTAAAAAAAAATAAACTTAAATAAGTTCGGGATATACAAAAAATGTGA
TGGCTCATAGCTTTTTCATACTAACACTTAAAGGATACAATATGTACTTG
CTTTGAACATGCATATAGAATACACACTTAGTTGTACAAAACTTAGACGA
TCTATTAATTACTACCGGAAAGTACTGACCGGACGACGTGAGATGATTTT
CTGTTTACAAGTTATTAATTTTACACATGGCCTTTGTTCTGCCATAAATG
GAGTCCTATCAAATCCATCAAACAATCATGTCATCACTTGTACGTTTCCA
ATCAAAGCATTGAATCAATAATGCCAAAGTCATGCTAATTGTTCGAAGAG
CCAAAGCTTCTCCAGGACACACTCTTCTTCCCATCCCAAATGCTCAATCA
CCTTTTCTTTGTCAAACCAATCCCCTTGCTTGAAGTTCTCCGGCTCTTGT
GGCATCCGGCTCCGAGACATCCATGCCTTAGATTGAATGGTCTGTGAATG
TCTCGTCAATACTTTATATCTGGCTCCTACTA

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