HO062590

EST Overview
NameHO062590
Unique NameHO062590
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length320
Libraries
Library NameType
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of HO062590 vs. TrEMBL
Match: Q9FGI3_ARATH (AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At5g37540 PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 6.400e-40
Identity = 77/105 (73.33%), Postives = 93/105 (88.57%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            +PTR  RPG++ TGSFYLG+NPNS+GF+YV +LTF +SQRMPNLDPLAYT+P+QGIRIG K+L+I  +VFR DAGGSGQTMVDSGSEFT+LV  AYDKV+ E+VR
Sbjct:  230 IPTRSNRPGLASTGSFYLGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVR 334
[Close]
BLAST of HO062590 vs. TrEMBL
Match: B9GUR2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_552931 PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 8.359e-40
Identity = 78/105 (74.29%), Postives = 90/105 (85.71%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            VPTRQ RPG +PTGSFYLG NPNS GFRY+ +LTFS+SQRMPNLDPLAYT+ MQGIRIG +KL+I  + FR D  G+GQTM+DSGSEFTYLV +AY+KVR EVVR
Sbjct:  230 VPTRQVRPGFTPTGSFYLGENPNSGGFRYINLLTFSQSQRMPNLDPLAYTVAMQGIRIGNQKLNIPISAFRPDPSGAGQTMIDSGSEFTYLVDEAYNKVREEVVR 334
[Close]
BLAST of HO062590 vs. TrEMBL
Match: D7MID8_ARALY (Aspartyl protease family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493732 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 4.149e-39
Identity = 76/105 (72.38%), Postives = 91/105 (86.67%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            +PTR  RPG++ TGSFYLG NPNS+GF+YV +LTF +SQRMPNLDPLAYT+P+ GIRIG K+L+I  +VFR DAGGSGQTMVDSGSEFT+LV  AYDKV+ E+VR
Sbjct:  231 IPTRSNRPGLASTGSFYLGENPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLLGIRIGQKRLNIPSSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVR 335
[Close]
BLAST of HO062590 vs. TrEMBL
Match: Q9C8C9_ARATH (Putative uncharacterized protein At1g66180 OS=Arabidopsis thaliana GN=At1g66180 PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 5.418e-39
Identity = 77/104 (74.04%), Postives = 90/104 (86.54%), Query Frame = -3 
Query:    7 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVV 318
            +P +  RPG +PTGSFYLG+NPNS GF+YV +LTF +SQRMPNLDPLAYT+PM GIR G KKL+IS +VFR DAGGSGQTMVDSGSEFT+LV  AYDKVRAE++
Sbjct:  219 IPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIM 322
[Close]
BLAST of HO062590 vs. TrEMBL
Match: Q8L8L6_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 5.418e-39
Identity = 77/104 (74.04%), Postives = 90/104 (86.54%), Query Frame = -3 
Query:    7 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVV 318
            +P +  RPG +PTGSFYLG+NPNS GF+YV +LTF +SQRMPNLDPLAYT+PM GIR G KKL+IS +VFR DAGGSGQTMVDSGSEFT+LV  AYDKVRAE++
Sbjct:  219 IPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIM 322
[Close]
BLAST of HO062590 vs. TrEMBL
Match: B9H3C4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555992 PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.207e-38
Identity = 76/105 (72.38%), Postives = 90/105 (85.71%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            VPTRQ RPG +PTGSFYLG NPNS GF+Y+ +LTFS+SQRMPNLDPLA+T+ +QGIRIG KKL+I  + FRAD  G+GQ+M+DSGSEFTYLV  AY+KVR EVVR
Sbjct:  225 VPTRQVRPGFTPTGSFYLGENPNSAGFQYISLLTFSQSQRMPNLDPLAHTVALQGIRIGNKKLNIPVSAFRADPSGAGQSMIDSGSEFTYLVDVAYNKVREEVVR 329
[Close]
BLAST of HO062590 vs. TrEMBL
Match: B9T2R1_RICCO (Aspartic proteinase nepenthesin-1, putative OS=Ricinus communis GN=RCOM_0593500 PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.071e-37
Identity = 74/105 (70.48%), Postives = 88/105 (83.81%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            VPTRQ R G+S TGSFYLGNNPNS  F+Y+ +LTF+ SQR PNLDPLAYTIPMQGIR+G  +L+IS  +FR D  G+GQT++DSGSEFTYLV +AY+KVR EVVR
Sbjct:  229 VPTRQARAGLSSTGSFYLGNNPNSGRFQYINLLTFTPSQRSPNLDPLAYTIPMQGIRMGNARLNISATLFRPDPSGAGQTIIDSGSEFTYLVDEAYNKVREEVVR 333
[Close]
BLAST of HO062590 vs. TrEMBL
Match: Q8RVE6_ORYSJ (Os01g0844500 protein OS=Oryza sativa subsp. japonica GN=P0406G08.38 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.243e-11
Identity = 32/71 (45.07%), Postives = 48/71 (67.61%), Query Frame = -3 
Query:    4 FSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 216
            +  +  +P  D +AY++ + GIR+GGK L I  +V   D  G+GQTMVDSG++FT+L+ DAY  ++AE  R
Sbjct:  248 YQPAMPLPYFDRVAYSVQLLGIRVGGKPLPIPASVLAPDHTGAGQTMVDSGTQFTFLLGDAYSALKAEFSR 318
[Close]
BLAST of HO062590 vs. TrEMBL
Match: A2WWX5_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04407 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.243e-11
Identity = 32/71 (45.07%), Postives = 48/71 (67.61%), Query Frame = -3 
Query:    4 FSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 216
            +  +  +P  D +AY++ + GIR+GGK L I  +V   D  G+GQTMVDSG++FT+L+ DAY  ++AE  R
Sbjct:  247 YQPAMPLPYFDRVAYSVQLLGIRVGGKPLPIPASVLAPDHTGAGQTMVDSGTQFTFLLGDAYSALKAEFSR 317
[Close]
BLAST of HO062590 vs. TrEMBL
Match: C5XPQ7_SORBI (Putative uncharacterized protein Sb03g039590 OS=Sorghum bicolor GN=Sb03g039590 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.153e-11
Identity = 31/65 (47.69%), Postives = 47/65 (72.31%), Query Frame = -3 
Query:    4 MPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 198
            +P  D +AY++ + GIR+GGK L I  +V   D  G+GQTMVDSG++FT+L+ DAY  ++AE ++
Sbjct:  255 LPYFDRVAYSVQLLGIRVGGKALPIPASVLAPDHTGAGQTMVDSGTQFTFLLGDAYSALKAEFLK 319
[Close]
BLAST of HO062590 vs. TAIR peptide
Match: AT5G37540.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr5:14912862-14914190 FORWARD LENGTH=442)

HSP 1 Score: 166.777 bits (421), Expect = 2.452e-42
Identity = 77/105 (73.33%), Postives = 93/105 (88.57%), Query Frame = -3 
Query:    4 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 318
            +PTR  RPG++ TGSFYLG+NPNS+GF+YV +LTF +SQRMPNLDPLAYT+P+QGIRIG K+L+I  +VFR DAGGSGQTMVDSGSEFT+LV  AYDKV+ E+VR
Sbjct:  230 IPTRSNRPGLASTGSFYLGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVR 334
[Close]
BLAST of HO062590 vs. TAIR peptide
Match: AT1G66180.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:24647221-24648513 FORWARD LENGTH=430)

HSP 1 Score: 163.696 bits (413), Expect = 2.076e-41
Identity = 77/104 (74.04%), Postives = 90/104 (86.54%), Query Frame = -3 
Query:    7 VPTRQTRPGISPTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVV 318
            +P +  RPG +PTGSFYLG+NPNS GF+YV +LTF +SQRMPNLDPLAYT+PM GIR G KKL+IS +VFR DAGGSGQTMVDSGSEFT+LV  AYDKVRAE++
Sbjct:  219 IPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIM 322
[Close]
BLAST of HO062590 vs. TAIR peptide
Match: AT2G39710.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr2:16562051-16563379 REVERSE LENGTH=442)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-12
Identity = 36/100 (36.00%), Postives = 58/100 (58.00%), Query Frame = -3 
Query:    7 YLGNNPNSKGFRYVGMLTFS------------KSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVV 270
            Y  +  +S GF  +G  ++S            +S  +P  D +AYT+ ++GIR+G K LS+  +VF  D  G+GQTMVDSG++FT+L+   Y  ++ E +
Sbjct:  215 YCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFI 314
[Close]
BLAST of HO062590 vs. TAIR peptide
Match: AT5G02190.1 (| Symbols: EMB24, ATASP38, PCS1 | Eukaryotic aspartyl protease family protein | chr5:435322-436683 FORWARD LENGTH=453)

HSP 1 Score: 60.4622 bits (145), Expect = 2.475e-10
Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = -3 
Query:   16 SQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRA 207
            S  +P  D +AYT+ + GI++ GK L I  +V   D  G+GQTMVDSG++FT+L+   Y  +R+
Sbjct:  259 STPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRS 322
[Close]
BLAST of HO062590 vs. TAIR peptide
Match: AT3G25700.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:9358937-9360295 FORWARD LENGTH=452)

HSP 1 Score: 50.447 bits (119), Expect = 2.561e-7
Identity = 30/94 (31.91%), Postives = 46/94 (48.94%), Query Frame = -3 
Query:    4 PTGSFYLGNNPNSKGFRYVGMLTFSKSQRMPNLDPLAYTIPMQGIRIGGKKLSISPAVFRADAGGSGQTMVDSGSEFTYLVSDAYDKVRAEVVR 285
            PT    +GN  +      +  L F+     P L P  Y + ++ + + G KL I P+++  D  G+G T+VDSG+   +L   AY  V A V R
Sbjct:  261 PTSYLIIGNGGDG-----ISKLFFTPLLTNP-LSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRR 348
The following BLAST results are available for this feature:
BLAST of HO062590 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9FGI3_ARATH6.400e-4073.33AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At... [more]
B9GUR2_POPTR8.359e-4074.29Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7MID8_ARALY4.149e-3972.38Aspartyl protease family protein OS=Arabidopsis ly... [more]
Q9C8C9_ARATH5.418e-3974.04Putative uncharacterized protein At1g66180 OS=Arab... [more]
Q8L8L6_ARATH5.418e-3974.04Putative uncharacterized protein OS=Arabidopsis th... [more]
B9H3C4_POPTR1.207e-3872.38Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9T2R1_RICCO5.071e-3770.48Aspartic proteinase nepenthesin-1, putative OS=Ric... [more]
Q8RVE6_ORYSJ6.243e-1145.07Os01g0844500 protein OS=Oryza sativa subsp. japoni... [more]
A2WWX5_ORYSI6.243e-1145.07Putative uncharacterized protein OS=Oryza sativa s... [more]
C5XPQ7_SORBI8.153e-1147.69Putative uncharacterized protein Sb03g039590 OS=So... [more]
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BLAST of HO062590 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G37540.12.452e-4273.33| Symbols: | Eukaryotic aspartyl protease family ... [more]
AT1G66180.12.076e-4174.04| Symbols: | Eukaryotic aspartyl protease family ... [more]
AT2G39710.11.187e-1236.00| Symbols: | Eukaryotic aspartyl protease family ... [more]
AT5G02190.12.475e-1043.75| Symbols: EMB24, ATASP38, PCS1 | Eukaryotic aspar... [more]
AT3G25700.12.561e-731.91| Symbols: | Eukaryotic aspartyl protease family ... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Peptidase A1PANTHERPTHR13683ASPARTYL PROTEASEScoord: 10..105
score: 6
IPR009007Peptidase aspartic, catalyticGENE3D2.40.70.10no descriptioncoord: 25..105
score: 1.1
IPR021109Peptidase asparticSUPERFAMILY50630Acid proteasescoord: 15..106
score: 5.1
NoneNo IPR availablePANTHERPTHR13683:SF98CHLOROPLAST NUCLEIOD DNA-BINDING-RELATEDcoord: 10..105
score: 6

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO062590 ID=HO062590; Name=HO062590; organism=Cicer arietinum; type=EST; length=320bp
AGCTCTAACCACTTCTGCCCGCACTTTATCATAAGCATCGCTAACTAAAT
ATGTGAATTCGGATCCGGAGTCAACCATGGTTTGACCCGACCCACCTGCA
TCGGCTCGGAAAACCGCCGGCGAAATGCTCAGTTTCTTCCCGCCGATTCT
TATACCTTGCATAGGGATAGTGTAAGCCAAGGGGTCGAGGTTCGGCATGC
GTTGACTCTTGGAAAATGTCAACATTCCGACGTAACGAAACCCTTTAGAA
TTCGGGTTGTTACCGAGGTAGAATGACCCGGTTGGAGAGATTCCGGGTCG
GGTTTGACGGGTTGGTACCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR009007Peptidase aspartic, catalytic
IPR021109Peptidase_aspartic_dom_sf