FE671410

EST Overview


Name	FE671410
Unique Name	FE671410
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	647

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE671410 vs. SwissProt
Match: KPYC_SOYBN (Pyruvate kinase, cytosolic isozyme OS=Glycine max PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.987e-20
Identity = 54/120 (45.00%), Postives = 78/120 (65.00%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV++    L+AGM+VARF+FS G  +YHQ+TL NLK ++ +T  LCAVMLDT G E++    K+   I L+   +V +T D   +   E++ +++  L   +K G+TI
Sbjct:   23 TKIVCTLGPASRSVEMTEKLLRAGMNVARFNFSHGTHDYHQETLNNLKTAMHNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKGDPEMISMSYKKLPVHLKPGNTI 142

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYC_SOLTU (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.095e-20
Identity = 51/120 (42.50%), Postives = 81/120 (67.50%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SR+V ++   L+AGM+VARF+FS G  EYHQ+TL+NLK ++++T+ LCAVMLDT G E++     +   I L+   ++ ++ D   + + E++ +++  L   +K G+TI
Sbjct:   22 TKIVCTLGPSSRTVPMLEKLLRAGMNVARFNFSHGTHEYHQETLDNLKIAMQNTQILCAVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGNEEMISMSYKKLVMDLKPGNTI 141

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYC_TOBAC (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 8.691e-20
Identity = 50/120 (41.67%), Postives = 79/120 (65.83%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV +I   L+AGM+VARF+FS G  +YHQ+T++NL+++++ST  LCAVMLDT G E++    K+   + L+   ++ ++ D   +    ++ +++  LA+ VK    I
Sbjct:   20 TKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGILCAVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKPQSVI 139

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYC_ARATH (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana GN=At4g26390 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.482e-19
Identity = 52/120 (43.33%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV ++   L AGMSVARF+FS G  EYHQ+TL+NL++++ +T  LCAVMLDT G E++    K+   I L+   ++ ++ D   +   + + +++  LA+ V  G  I
Sbjct:    9 TKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGMVI 128

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK1_SALTY (Pyruvate kinase I OS=Salmonella typhimurium GN=pykF PE=3 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 3.091e-17
Identity = 47/135 (34.81%), Postives = 78/135 (57.78%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVN-KNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSV 642
            TKIV T+GPK+ S +++S  L AGM+V R +FS GD   H Q ++NL+  +  T K  A++LDT G E++ +  +    +SL+A      T D+    ++EI+ + ++G    +  G+T+ V   L  G E T++
Sbjct:    4 TKIVCTIGPKTESEEMLSKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKKAAILLDTKGPEIRTIKLEGGNDVSLKAGQTFTFTTDKSVVGNNEIVAVTYEGFTSDLSVGNTVLVDDGL-IGMEVTAI 137

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK1_SALTI (Pyruvate kinase I OS=Salmonella typhi GN=pykF PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.091e-17
Identity = 47/135 (34.81%), Postives = 78/135 (57.78%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVN-KNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSV 642
            TKIV T+GPK+ S +++S  L AGM+V R +FS GD   H Q ++NL+  +  T K  A++LDT G E++ +  +    +SL+A      T D+    ++EI+ + ++G    +  G+T+ V   L  G E T++
Sbjct:    4 TKIVCTIGPKTESEEMLSKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKKAAILLDTKGPEIRTIKLEGGNDVSLKAGQTFTFTTDKSVVGNNEIVAVTYEGFTSDLSVGNTVLVDDGL-IGMEVTAI 137

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK1_ECOLI (Pyruvate kinase I OS=Escherichia coli (strain K12) GN=pykF PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.534e-16
Identity = 46/135 (34.07%), Postives = 78/135 (57.78%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVN-KNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSV 642
            TKIV T+GPK+ S ++++  L AGM+V R +FS GD   H Q ++NL+  +  T K  A++LDT G E++ +  +    +SL+A      T D+    +SE++ + ++G    +  G+T+ V   L  G E T++
Sbjct:    4 TKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGL-IGMEVTAI 137

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK1_ECO57 (Pyruvate kinase I OS=Escherichia coli O157:H7 GN=pykF PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.534e-16
Identity = 46/135 (34.07%), Postives = 78/135 (57.78%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVN-KNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSV 642
            TKIV T+GPK+ S ++++  L AGM+V R +FS GD   H Q ++NL+  +  T K  A++LDT G E++ +  +    +SL+A      T D+    +SE++ + ++G    +  G+T+ V   L  G E T++
Sbjct:    4 TKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGL-IGMEVTAI 137

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK_THELI (Pyruvate kinase (Fragment) OS=Thermococcus litoralis GN=pki PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 5.830e-16
Identity = 49/128 (38.28%), Postives = 74/128 (57.81%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTG 624
            TKI+ T+GP S+  + I   +KAGMSVAR +FS G  E H +T+E +++  +  ++  A++ D  G +M+V      S++L+   +VVLT  +  E     +P+ F  L K V KGDTI    YLS G
Sbjct:   12 TKIIATIGPASKQKESIKKMIKAGMSVARINFSHGTLEEHAKTIETVRDVAEKLERRVAILGDLPGLKMRVGKIKGDSVTLRKGDKVVLT-TRDIEGDETTIPVEFKDLPKLVSKGDTI----YLSDG 134

[Close]

BLAST of FE671410 vs. SwissProt
Match: KPYK_BACLI (Pyruvate kinase OS=Bacillus licheniformis GN=pyk PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.299e-15
Identity = 48/134 (35.82%), Postives = 77/134 (57.46%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSV 642
            TKIV T+GP S SV+ ++  ++AGM+VAR +FS GD E H   ++N++E+     K   ++LDT G E++       SI L A SQ++++ D+    + + + + +DGL   V  G TI +   L  G E T +
Sbjct:    4 TKIVCTIGPASESVEKLTQLMEAGMNVARLNFSHGDFEEHGARIKNIREAAGKLGKDIGILLDTKGPEIRTHTMENGSIELAAGSQLIVSMDE-VIGTPDKISVTYDGLIHDVSVGSTILLDDGL-VGLEVTDI 135

[Close]

BLAST of FE671410 vs. TrEMBL
Match: B9SRM0_RICCO (Pyruvate kinase OS=Ricinus communis GN=RCOM_0840740 PE=3 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 8.878e-73
Identity = 136/164 (82.93%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPA+TKIVGTLGPKSRS+DVIS CLK+GMSVARFDFSWGD EYHQ+TLENLK ++KSTKKLCAVMLDTVG E+QVVNK+E SISL A+  VVLTP+Q QEASSE+LPINFDGL+K+VK GDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMASILEPSKSSFFPAMTKIVGTLGPKSRSIDVISGCLKSGMSVARFDFSWGDPEYHQETLENLKAAVKSTKKLCAVMLDTVGPELQVVNKSEKSISLLAEGVVVLTPNQDQEASSELLPINFDGLSKAVKPGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q2QXR8_ORYSJ (Pyruvate kinase OS=Oryza sativa subsp. japonica GN=Os12g0145700 PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.514e-72
Identity = 136/164 (82.93%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHS++LL+E+PIRM SILEPSK SFFPA+TKIVGTLGPKSRSVD ISSCLKAGMSVARFDFSWGD+EYHQ+TLENLK +IKSTKKLCAVMLDTVG E+QVVNK+E SISL+ +  V+LTPDQGQEASS++LPINF GLAK+VK GDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSTNLLLEEPIRMASILEPSKPSFFPAMTKIVGTLGPKSRSVDTISSCLKAGMSVARFDFSWGDAEYHQETLENLKVAIKSTKKLCAVMLDTVGPELQVVNKSEASISLEENGTVILTPDQGQEASSQVLPINFAGLAKAVKPGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q2QXR7_ORYSJ (Pyruvate kinase OS=Oryza sativa subsp. japonica GN=LOC_Os12g05110 PE=3 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.514e-72
Identity = 136/164 (82.93%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHS++LL+E+PIRM SILEPSK SFFPA+TKIVGTLGPKSRSVD ISSCLKAGMSVARFDFSWGD+EYHQ+TLENLK +IKSTKKLCAVMLDTVG E+QVVNK+E SISL+ +  V+LTPDQGQEASS++LPINF GLAK+VK GDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSTNLLLEEPIRMASILEPSKPSFFPAMTKIVGTLGPKSRSVDTISSCLKAGMSVARFDFSWGDAEYHQETLENLKVAIKSTKKLCAVMLDTVGPELQVVNKSEASISLEENGTVILTPDQGQEASSQVLPINFAGLAKAVKPGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q1SN32_MEDTR (Pyruvate kinase OS=Medicago truncatula GN=MtrDRAFT_AC139525g8v2 PE=3 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.514e-72
Identity = 138/164 (84.15%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            M SSHLL+E+PIRM SILEPSKASFF A+TKIVGTLGPKSRSV+ IS CLKAGMSVARFDFSW D EYHQ+TLENLK +IK TKKLCAVMLDTVGAEMQVVNK+ET+ISL+ D+QVVLTP+QGQEASSEILPINFDGLA++VK GDTIF+GQYL TGSETTSVW
Sbjct:    1 MPSSHLLLEEPIRMASILEPSKASFFSAMTKIVGTLGPKSRSVETISGCLKAGMSVARFDFSWCDPEYHQETLENLKTAIKGTKKLCAVMLDTVGAEMQVVNKSETTISLEIDAQVVLTPNQGQEASSEILPINFDGLAQAVKTGDTIFIGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: B8BM17_ORYSI (Pyruvate kinase OS=Oryza sativa subsp. indica GN=OsI_37456 PE=3 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.514e-72
Identity = 136/164 (82.93%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHS++LL+E+PIRM SILEPSK SFFPA+TKIVGTLGPKSRSVD ISSCLKAGMSVARFDFSWGD+EYHQ+TLENLK +IKSTKKLCAVMLDTVG E+QVVNK+E SISL+ +  V+LTPDQGQEASS++LPINF GLAK+VK GDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSTNLLLEEPIRMASILEPSKPSFFPAMTKIVGTLGPKSRSVDTISSCLKAGMSVARFDFSWGDAEYHQETLENLKVAIKSTKKLCAVMLDTVGPELQVVNKSEASISLEENGTVILTPDQGQEASSQVLPINFAGLAKAVKPGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q9SJQ0_ARATH (Pyruvate kinase OS=Arabidopsis thaliana GN=At2g36580 PE=2 SV=2)

HSP 1 Score: 274.633 bits (701), Expect = 4.406e-72
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+VI+ CLKAGMSVARFDFSW D++YHQ+TLENLK ++KSTKKLCAVMLDTVG E+QV+NK E +ISL+AD  V LTP Q QEASSE+LPINFDGLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMTSILEPSKSSFFPALTKIVGTLGPKSRSVEVIAGCLKAGMSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAVMLDTVGPELQVINKTEKAISLKADGLVTLTPSQDQEASSEVLPINFDGLAKAVKKGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q8LFE1_ARATH (Pyruvate kinase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 4.406e-72
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+VI+ CLKAGMSVARFDFSW D++YHQ+TLENLK ++KSTKKLCAVMLDTVG E+QV+NK E +ISL+AD  V LTP Q QEASSE+LPINFDGLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMTSILEPSKSSFFPALTKIVGTLGPKSRSVEVIAGCLKAGMSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAVMLDTVGPELQVINKTEKAISLKADGLVTLTPSQDQEASSEVLPINFDGLAKAVKKGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q8H176_ARATH (Pyruvate kinase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 4.406e-72
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+VI+ CLKAGMSVARFDFSW D++YHQ+TLENLK ++KSTKKLCAVMLDTVG E+QV+NK E +ISL+AD  V LTP Q QEASSE+LPINFDGLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMTSILEPSKSSFFPALTKIVGTLGPKSRSVEVIAGCLKAGMSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAVMLDTVGPELQVINKTEKAISLKADGLVTLTPSQDQEASSEVLPINFDGLAKAVKKGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: D7LJ52_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_902775 PE=4 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 7.516e-72
Identity = 135/164 (82.32%), Postives = 150/164 (91.46%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+VIS CLKAGMSVARFDFSW D+EYHQ+TLENLK ++KSTKKLCAVMLDTVG E+QV+NK E +ISL+AD  V LTP Q QEASSE+ PINFDGLAK+VK+GDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMTSILEPSKSSFFPALTKIVGTLGPKSRSVEVISGCLKAGMSVARFDFSWCDAEYHQETLENLKIAVKSTKKLCAVMLDTVGPELQVINKTEKAISLKADGLVTLTPSQDQEASSEVFPINFDGLAKAVKQGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TrEMBL
Match: Q2RAK2_ORYSJ (Pyruvate kinase OS=Oryza sativa subsp. japonica GN=Os11g0148500 PE=2 SV=1)

HSP 1 Score: 273.092 bits (697), Expect = 1.282e-71
Identity = 135/164 (82.32%), Postives = 152/164 (92.68%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHS++LL+E+PIRM SILEPSK SFFPA+TKIVGTLGPKSR+VD ISSCLKAGMSVARFDFSWGD+EYHQ+TLENLK +IKSTKKLCAVMLDTVG E+QVVNK+E +ISL+A+  VVLTPDQGQEASSE+LPINF GLAK++K G TIFVGQYL TGSETTSVW
Sbjct:    1 MHSTNLLLEEPIRMASILEPSKPSFFPAMTKIVGTLGPKSRAVDTISSCLKAGMSVARFDFSWGDAEYHQETLENLKLAIKSTKKLCAVMLDTVGPELQVVNKSEAAISLEANGTVVLTPDQGQEASSELLPINFSGLAKALKPGATIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT2G36580.1 (| Symbols: | Pyruvate kinase family protein | chr2:15339253-15342781 FORWARD LENGTH=527)

HSP 1 Score: 274.633 bits (701), Expect = 3.114e-74
Identity = 135/164 (82.32%), Postives = 151/164 (92.07%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+VI+ CLKAGMSVARFDFSW D++YHQ+TLENLK ++KSTKKLCAVMLDTVG E+QV+NK E +ISL+AD  V LTP Q QEASSE+LPINFDGLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMTSILEPSKSSFFPALTKIVGTLGPKSRSVEVIAGCLKAGMSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAVMLDTVGPELQVINKTEKAISLKADGLVTLTPSQDQEASSEVLPINFDGLAKAVKKGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT3G52990.1 (| Symbols: | Pyruvate kinase family protein | chr3:19649046-19652237 FORWARD LENGTH=527)

HSP 1 Score: 272.707 bits (696), Expect = 1.183e-73
Identity = 131/164 (79.88%), Postives = 153/164 (93.29%), Query Frame = 1

Query:  154 MHSSHLLIEKPIRMISILEPSKASFFPALTKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MHSSHLL+E+PIRM SILEPSK+SFFPALTKIVGTLGPKSRSV+ +S CLKAGMSVARFDFSWGD++YHQ+TL+NLK +++STKKLCAVMLDTVG E+QV+NK+E +I+L+AD  V LTP+Q QEASSE+LPINF+GLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MHSSHLLLEEPIRMASILEPSKSSFFPALTKIVGTLGPKSRSVEALSGCLKAGMSVARFDFSWGDADYHQETLDNLKVAVRSTKKLCAVMLDTVGPELQVINKSEKAITLKADGLVTLTPNQDQEASSEVLPINFNGLAKAVKKGDTIFVGQYLFTGSETTSVW 164

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT3G52990.2 (| Symbols: | Pyruvate kinase family protein | chr3:19649336-19652237 FORWARD LENGTH=474)

HSP 1 Score: 182.57 bits (462), Expect = 1.610e-46
Identity = 86/111 (77.48%), Postives = 103/111 (92.79%), Query Frame = 1

Query:  313 MSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQVVNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTIFVGQYLSTGSETTSVW 645
            MSVARFDFSWGD++YHQ+TL+NLK +++STKKLCAVMLDTVG E+QV+NK+E +I+L+AD  V LTP+Q QEASSE+LPINF+GLAK+VKKGDTIFVGQYL TGSETTSVW
Sbjct:    1 MSVARFDFSWGDADYHQETLDNLKVAVRSTKKLCAVMLDTVGPELQVINKSEKAITLKADGLVTLTPNQDQEASSEVLPINFNGLAKAVKKGDTIFVGQYLFTGSETTSVW 111

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT3G04050.1 (| Symbols: | Pyruvate kinase family protein | chr3:1049795-1051522 FORWARD LENGTH=510)

HSP 1 Score: 99.3673 bits (246), Expect = 1.793e-21
Identity = 52/120 (43.33%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV+++   LKAGM+VARF+FS G   YHQ+TL+NL+ ++++T   CAVMLDT G E++    K    + L    ++ ++ D   E  S  + +++  LA+ +K GD I
Sbjct:   18 TKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCAVMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKSGDVI 137

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT3G55650.1 (| Symbols: | Pyruvate kinase family protein | chr3:20647085-20648617 FORWARD LENGTH=510)

HSP 1 Score: 98.9821 bits (245), Expect = 2.341e-21
Identity = 54/120 (45.00%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKI+ TLGP SRSV++I   LKAGM+VARF+FS G   YHQ+TL+NL+ ++ +T  L AVMLDT G E++    K    I L    ++ ++ D   E  S ++ +++  LA+ VK GD I
Sbjct:   18 TKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILSAVMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISMSYKKLAEDVKPGDVI 137

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT5G63680.1 (| Symbols: | Pyruvate kinase family protein | chr5:25490507-25492530 FORWARD LENGTH=510)

HSP 1 Score: 98.5969 bits (244), Expect = 3.058e-21
Identity = 54/120 (45.00%), Postives = 78/120 (65.00%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV +I   LKAGM+VARF+FS G  EYHQ+TL+NL+ ++++T  L AVMLDT G E++    K+   I L+   ++ +T D   +   + + +++  L   VK G+TI
Sbjct:   22 TKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKLPVDVKPGNTI 141

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT5G08570.1 (| Symbols: | Pyruvate kinase family protein | chr5:2778433-2780300 FORWARD LENGTH=510)

HSP 1 Score: 97.4413 bits (241), Expect = 6.813e-21
Identity = 53/120 (44.17%), Postives = 76/120 (63.33%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SR+V +I   LKAGM+VARF+FS G  EYHQ+TL+NL+ ++ +T  L AVMLDT G E++    K+   I L+   ++ +T D   +     + +++  L   VK G+TI
Sbjct:   22 TKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKLPLDVKPGNTI 141

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT4G26390.1 (| Symbols: | Pyruvate kinase family protein | chr4:13342207-13344418 FORWARD LENGTH=497)

HSP 1 Score: 96.2857 bits (238), Expect = 1.518e-20
Identity = 52/120 (43.33%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV ++   L AGMSVARF+FS G  EYHQ+TL+NL++++ +T  LCAVMLDT G E++    K+   I L+   ++ ++ D   +   + + +++  LA+ V  G  I
Sbjct:    9 TKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGMVI 128

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT3G25960.1 (| Symbols: | Pyruvate kinase family protein | chr3:9498439-9499932 FORWARD LENGTH=497)

HSP 1 Score: 95.5153 bits (236), Expect = 2.589e-20
Identity = 52/120 (43.33%), Postives = 76/120 (63.33%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV++I   LKAGM+VARF+FS G   YHQ+TL+NL+ ++ +T  LCAVMLDT    ++    K    I L+   ++ ++ D   +  S  + +++  LA+ +K GD I
Sbjct:   18 TKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILCAVMLDTKSPVIRTGFLKEGKPIQLKQGQEITISIDYKIQGDSNTISMSYKKLAEDLKPGDVI 137

[Close]

BLAST of FE671410 vs. TAIR peptide
Match: AT5G56350.1 (| Symbols: | Pyruvate kinase family protein | chr5:22820254-22822529 REVERSE LENGTH=498)

HSP 1 Score: 93.2041 bits (230), Expect = 1.285e-19
Identity = 51/120 (42.50%), Postives = 75/120 (62.50%), Query Frame = 1

Query:  241 TKIVGTLGPKSRSVDVISSCLKAGMSVARFDFSWGDSEYHQQTLENLKESIKSTKKLCAVMLDTVGAEMQV-VNKNETSISLQADSQVVLTPDQGQEASSEILPINFDGLAKSVKKGDTI 597
            TKIV TLGP SRSV ++   L+AGM+VARF+FS G  EYHQ+TL+NL +++ +T  LCAVMLDT G E++    K+   I L+   ++ ++ D   +     + +++  LA  V  G  I
Sbjct:   10 TKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLHQAMLNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVDVNPGMVI 129

The following BLAST results are available for this feature:

BLAST of FE671410 vs. SwissProt
BLAST of FE671410 vs. TrEMBL
BLAST of FE671410 vs. TAIR peptide

BLAST of FE671410 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
KPYC_SOYBN	2.987e-20	45.00	Pyruvate kinase, cytosolic isozyme OS=Glycine max ...	[more]
KPYC_SOLTU	5.095e-20	42.50	Pyruvate kinase, cytosolic isozyme OS=Solanum tube...	[more]
KPYC_TOBAC	8.691e-20	41.67	Pyruvate kinase, cytosolic isozyme OS=Nicotiana ta...	[more]
KPYC_ARATH	1.482e-19	43.33	Probable pyruvate kinase, cytosolic isozyme OS=Ara...	[more]
KPYK1_SALTY	3.091e-17	34.81	Pyruvate kinase I OS=Salmonella typhimurium GN=pyk...	[more]
KPYK1_SALTI	3.091e-17	34.81	Pyruvate kinase I OS=Salmonella typhi GN=pykF PE=3...	[more]
KPYK1_ECOLI	1.534e-16	34.07	Pyruvate kinase I OS=Escherichia coli (strain K12)...	[more]
KPYK1_ECO57	1.534e-16	34.07	Pyruvate kinase I OS=Escherichia coli O157:H7 GN=p...	[more]
KPYK_THELI	5.830e-16	38.28	Pyruvate kinase (Fragment) OS=Thermococcus litoral...	[more]
KPYK_BACLI	1.299e-15	35.82	Pyruvate kinase OS=Bacillus licheniformis GN=pyk P...	[more]

BLAST of FE671410 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SRM0_RICCO	8.878e-73	82.93	Pyruvate kinase OS=Ricinus communis GN=RCOM_084074...	[more]
Q2QXR8_ORYSJ	1.514e-72	82.93	Pyruvate kinase OS=Oryza sativa subsp. japonica GN...	[more]
Q2QXR7_ORYSJ	1.514e-72	82.93	Pyruvate kinase OS=Oryza sativa subsp. japonica GN...	[more]
Q1SN32_MEDTR	1.514e-72	84.15	Pyruvate kinase OS=Medicago truncatula GN=MtrDRAFT...	[more]
B8BM17_ORYSI	1.514e-72	82.93	Pyruvate kinase OS=Oryza sativa subsp. indica GN=O...	[more]
Q9SJQ0_ARATH	4.406e-72	82.32	Pyruvate kinase OS=Arabidopsis thaliana GN=At2g365...	[more]
Q8LFE1_ARATH	4.406e-72	82.32	Pyruvate kinase OS=Arabidopsis thaliana PE=2 SV=1	[more]
Q8H176_ARATH	4.406e-72	82.32	Pyruvate kinase OS=Arabidopsis thaliana PE=2 SV=1	[more]
D7LJ52_ARALY	7.516e-72	82.32	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q2RAK2_ORYSJ	1.282e-71	82.32	Pyruvate kinase OS=Oryza sativa subsp. japonica GN...	[more]

BLAST of FE671410 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G36580.1	3.114e-74	82.32	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G52990.1	1.183e-73	79.88	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G52990.2	1.610e-46	77.48	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G04050.1	1.793e-21	43.33	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G55650.1	2.341e-21	45.00	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G63680.1	3.058e-21	45.00	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G08570.1	6.813e-21	44.17	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT4G26390.1	1.518e-20	43.33	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G25960.1	2.589e-20	43.33	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G56350.1	1.285e-19	42.50	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 80..205 score: 2.4
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 81..201 score: 2.7
IPR015813	Pyruvate/Phosphoenolpyruvate kinase	GENE3D	3.20.20.60	no description	coord: 81..152 score: 7.6
IPR015813	Pyruvate/Phosphoenolpyruvate kinase	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 49..151 score: 1.9
None	No IPR available	SEG	seg	seg	coord: 7..30 score
None	No IPR available	SEG	seg	seg	coord: 2..14 score
None	No IPR available	SEG	seg	seg	coord: 26..37 score
None	No IPR available	SEG	seg	seg	coord: 39..60 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671410 ID=FE671410; Name=FE671410; organism=Cicer arietinum; type=EST; length=647bp
ATTCCACTCATCCAACGTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC
TTCTTCTTCTTCTTCTTCTACTACTACTACTACTTCCTCCTTCGCTCGTT
CGTTTACTAGGGTTCTGATTCAAAAACCACATCAACATCAACAACAAGCA
AAAATGCATTCCAGTCACTTGCTTATCGAAAAACCAATTCGGATGATCTC
AATCCTCGAACCATCCAAAGCCAGCTTCTTTCCTGCTTTGACAAAGATCG
TTGGTACTCTTGGACCTAAATCTCGCTCAGTCGATGTTATTTCTTCTTGT
CTCAAAGCTGGAATGTCTGTGGCTCGTTTTGATTTCTCTTGGGGTGACTC
TGAATATCACCAACAGACTTTGGAGAATTTGAAGGAGTCTATTAAGAGTA
CTAAGAAGCTCTGTGCTGTTATGTTGGACACAGTGGGTGCTGAGATGCAG
GTTGTTAACAAAAACGAGACATCAATTTCGCTTCAGGCTGATAGTCAGGT
TGTNTTAACTCCTGATCAGGGACAAGAAGCTTCTTCAGAGATATTGCCTA
TCAATTTTGATGGATTGGCCAAGTCAGTGAAGAAGGGAGACACCATTTTC
GTTGGTCAATACTTGTCCACAGGAAGTGAAACTACTTCTGTATGGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0004743	pyruvate kinase activity
GO:0030955	potassium ion binding
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001697	Pyr_Knase
IPR015793	Pyrv_Knase_brl
IPR015813	Pyrv/PenolPyrv_Kinase-like_dom

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