FE671472

EST Overview


Name	FE671472
Unique Name	FE671472
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	606

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE671472 vs. SwissProt
Match: CPR4_CAEEL (Cathepsin B-like cysteine proteinase 4 OS=Caenorhabditis elegans GN=cpr-4 PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.746e-40
Identity = 76/138 (55.07%), Postives = 94/138 (68.12%), Query Frame = -3

Query:  167 PGC-EPTYRTPKCVKKCVNEN--LLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLP 571
            P C +  Y TP CV KC N+N  + +   KHF   AY +      I AE+  +GPVE +FTVYEDF  YK+GVY H TG ELGGHA++++GWGT D+G  YWL AN WN NWG++GYF+I RGTNECGIE  V  G+P
Sbjct:  197 PSCPDDGYDTPACVNKCTNKNYNVAYTADKHFGSTAYAVGKKVSQIQAEIIAHGPVEAAFTVYEDFYQYKTGVYVHTTGQELGGHAIRILGWGT-DNGTPYWLVANSWNVNWGENGYFRIIRGTNECGIEHAVVGGVP 333

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BLAST of FE671472 vs. SwissProt
Match: CATB_PIG (Cathepsin B OS=Sus scrofa GN=CTSB PE=1 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.521e-39
Identity = 72/148 (48.65%), Postives = 99/148 (66.89%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKCV-NENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TPKC K C       ++E KHF   +Y+++ + ++IMAE+YKNGPVE +FTVY DF  YKSGVY+H+TG  +GGHA++++GWG  ++G  YWL  N WN +WGD+G+FKI RG + CGIE  + AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCSKICEPGYTPSYKEDKHFGCSSYSISRNEKEIMAEIYKNGPVEGAFTVYSDFLQYKSGVYQHVTGDLMGGHAIRILGWGV-ENGTPYWLVGNSWNTDWGDNGFFKILRGQDHCGIESEIVAGIPCT 332

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BLAST of FE671472 vs. SwissProt
Match: CATB_PONAB (Cathepsin B OS=Pongo abelii GN=CTSB PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.292e-39
Identity = 72/148 (48.65%), Postives = 101/148 (68.24%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKCV-NENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TPKC K C    +  +++ KH+   +Y++++  +DIMAE+YKNGPVE +F+VY DF  YKSGVY+H+TG  +GGHA++++GWG  ++G  YWL AN WN +WGD+G+FKI RG + CGIE  V AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSERDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGV-ENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRT 332

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BLAST of FE671472 vs. SwissProt
Match: CATB_MACFA (Cathepsin B OS=Macaca fascicularis GN=CTSB PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.292e-39
Identity = 72/148 (48.65%), Postives = 101/148 (68.24%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKCV-NENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TPKC K C    +  +++ KH+   +Y++++  +DIMAE+YKNGPVE +F+VY DF  YKSGVY+H+TG  +GGHA++++GWG  ++G  YWL AN WN +WGD+G+FKI RG + CGIE  V AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGV-ENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRT 332

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BLAST of FE671472 vs. SwissProt
Match: CATB_HUMAN (Cathepsin B OS=Homo sapiens GN=CTSB PE=1 SV=3)

HSP 1 Score: 161.384 bits (407), Expect = 3.292e-39
Identity = 72/148 (48.65%), Postives = 101/148 (68.24%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKCV-NENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TPKC K C    +  +++ KH+   +Y++++  +DIMAE+YKNGPVE +F+VY DF  YKSGVY+H+TG  +GGHA++++GWG  ++G  YWL AN WN +WGD+G+FKI RG + CGIE  V AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGV-ENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRT 332

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BLAST of FE671472 vs. SwissProt
Match: CATB_RAT (Cathepsin B OS=Rattus norvegicus GN=Ctsb PE=1 SV=2)

HSP 1 Score: 159.458 bits (402), Expect = 1.251e-38
Identity = 72/149 (48.32%), Postives = 99/149 (66.44%), Query Frame = -3

Query:  158 PYFDEIGCSHPGCEPTYRTPKCVKKC-VNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTK 601
            P    +  S P C     TPKC K C    +  ++E KH+   +Y+++   ++IMAE+YKNGPVE +FTV+ DF  YKSGVYKH  G  +GGHA++++GWG  ++G  YWL AN WN +WGD+G+FKI RG N CGIE  + AG+P T+
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCNKMCEAGYSTSYKEDKHYGYTSYSVSDSEKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDVMGGHAIRILGWGI-ENGVPYWLVANSWNVDWGDNGFFKILRGENHCGIESEIVAGIPRTQ 333

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BLAST of FE671472 vs. SwissProt
Match: CATB_MOUSE (Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2)

HSP 1 Score: 159.073 bits (401), Expect = 1.634e-38
Identity = 72/148 (48.65%), Postives = 99/148 (66.89%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKC-VNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TP+C K C    +  ++E KHF   +Y++++  ++IMAE+YKNGPVE +FTV+ DF  YKSGVYKH  G  +GGHA++++GWG  ++G  YWL AN WN +WGD+G+FKI RG N CGIE  + AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGV-ENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRT 332

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BLAST of FE671472 vs. SwissProt
Match: CATB_BOVIN (Cathepsin B OS=Bos taurus GN=CTSB PE=1 SV=5)

HSP 1 Score: 158.303 bits (399), Expect = 2.787e-38
Identity = 70/148 (47.30%), Postives = 100/148 (67.57%), Query Frame = -3

Query:  161 PYFDEIGCSHPGCEPTYRTPKCVKKCV-NENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFT 601
            P    +  S P C     TPKC K C    +  ++E KHF   +Y++ ++ ++IMAE+YKNGPVE +F+VY DF  YKSGVY+H++G  +GGHA++++GWG  ++G  YWL  N WN +WGD+G+FKI RG + CGIE  + AG+P T
Sbjct:  186 PCEHHVNGSRPPCTGEGDTPKCSKTCEPGYSPSYKEDKHFGCSSYSVANNEKEIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVSGEIMGGHAIRILGWGV-ENGTPYWLVGNSWNTDWGDNGFFKILRGQDHCGIESEIVAGMPCT 332

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BLAST of FE671472 vs. SwissProt
Match: CPR5_CAEEL (Cathepsin B-like cysteine proteinase 5 OS=Caenorhabditis elegans GN=cpr-5 PE=1 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 6.865e-37
Identity = 73/143 (51.05%), Postives = 89/143 (62.24%), Query Frame = -3

Query:  167 GCSHPGC-EPTYRTPKCVKKCVNENLL---WEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLP 583
            G   P C E T  TPKCV  C ++N     + + KHF   AY +    + I  E+  NGP+EV+FTVYEDF  Y +GVY H  G  LGGHAVK++GWG  D+G  YWL AN WN  WG+ GYF+I RG NECGIE    AG+P
Sbjct:  197 GVKWPACPEDTEPTPKCVDSCTSKNNYATPYLQDKHFGSTAYAVGKKVEQIQTEILTNGPIEVAFTVYEDFYQYTTGVYVHTAGASLGGHAVKILGWGV-DNGTPYWLVANSWNVAWGEKGYFRIIRGLNECGIEHSAVAGIP 338

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BLAST of FE671472 vs. SwissProt
Match: CPR1_CAEEL (Gut-specific cysteine proteinase OS=Caenorhabditis elegans GN=cpr-1 PE=1 SV=2)

HSP 1 Score: 152.91 bits (385), Expect = 1.171e-36
Identity = 71/137 (51.82%), Postives = 89/137 (64.96%), Query Frame = -3

Query:  173 CSHPGCEPTYRTPKCVKKCVNE-NLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAG 580
            C+   C P  +TP C   C +  +  + + KHF V AY +  +   I AE+Y NGPVE +F+VYEDF  YKSGVYKH  G  LGGHA+K+IGWGT + G  YWL AN W  NWG+ G+FKI RG ++CGIE  V AG
Sbjct:  191 CTSGNC-PESKTPSCSMSCQSGYSTAYAKDKHFGVSAYAVPKNAASIQAEIYANGPVEAAFSVYEDFYKYKSGVYKHTAGKYLGGHAIKIIGWGT-ESGSPYWLVANSWGVNWGESGFFKIYRGDDQCGIESAVVAG 325

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BLAST of FE671472 vs. TrEMBL
Match: Q2HV10_MEDTR (Peptidase C1A, papain; Somatotropin hormone; Peptidase C1, propeptide OS=Medicago truncatula GN=MtrDRAFT_AC149038g2v2 PE=3 SV=1)

HSP 1 Score: 308.916 bits (790), Expect = 1.791e-82
Identity = 138/162 (85.19%), Postives = 148/162 (91.36%), Query Frame = -3

Query:  119 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVTDMGAD 604
            DPYFD+IGCSHPGCEPTYRTPKCVKKCVN N LWE SKH+SVKAYT+NSDPQDIMAEVYKNGPVEV+FTVYEDF+HYKSGVYKHITG+ LGGHAVKL+GWGTS +GEDYWL ANQWN NWGDDGYFKIKRGTNECGIE  VTAGLP TKN ++ EVTDM  D
Sbjct:  191 DPYFDQIGCSHPGCEPTYRTPKCVKKCVNGNQLWETSKHYSVKAYTVNSDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKHITGFALGGHAVKLVGWGTSHEGEDYWLLANQWNTNWGDDGYFKIKRGTNECGIENAVTAGLPSTKN-IVREVTDMDVD 351

[Close]

BLAST of FE671472 vs. TrEMBL
Match: Q2HV09_MEDTR (Peptidase C1A, papain; Somatotropin hormone; Peptidase C1, propeptide OS=Medicago truncatula GN=MtrDRAFT_AC149038g3v2 PE=3 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 2.677e-78
Identity = 131/162 (80.86%), Postives = 144/162 (88.89%), Query Frame = -3

Query:  119 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVTDMGAD 604
            DPYFD+IGCSHPGCEP Y+TPKCV+KCV  N +W+ SKH+SVKAY + SDPQDIMAEVYKNGPVEV+FTV+EDF+HYKSGVYKHITG  LGGHAVKLIGWGTSD+GEDYWL ANQWN NWGDDGYFKIKRGTNECGIE  VTAGLP TKN ++ EVTDM  D
Sbjct:  192 DPYFDQIGCSHPGCEPAYQTPKCVRKCVKGNQIWKRSKHYSVKAYRVKSDPQDIMAEVYKNGPVEVAFTVFEDFAHYKSGVYKHITGSALGGHAVKLIGWGTSDEGEDYWLLANQWNTNWGDDGYFKIKRGTNECGIEDDVTAGLPSTKN-IVREVTDMDVD 352

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BLAST of FE671472 vs. TrEMBL
Match: B7FJ05_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 2.677e-78
Identity = 131/162 (80.86%), Postives = 144/162 (88.89%), Query Frame = -3

Query:  119 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVTDMGAD 604
            DPYFD+IGCSHPGCEP Y+TPKCV+KCV  N +W+ SKH+SVKAY + SDPQDIMAEVYKNGPVEV+FTV+EDF+HYKSGVYKHITG  LGGHAVKLIGWGTSD+GEDYWL ANQWN NWGDDGYFKIKRGTNECGIE  VTAGLP TKN ++ EVTDM  D
Sbjct:  194 DPYFDQIGCSHPGCEPAYQTPKCVRKCVKGNQIWKRSKHYSVKAYRVKSDPQDIMAEVYKNGPVEVAFTVFEDFAHYKSGVYKHITGSALGGHAVKLIGWGTSDEGEDYWLLANQWNTNWGDDGYFKIKRGTNECGIEDDVTAGLPSTKN-IVREVTDMDVD 354

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BLAST of FE671472 vs. TrEMBL
Match: B7FK90_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 7.788e-78
Identity = 130/162 (80.25%), Postives = 143/162 (88.27%), Query Frame = -3

Query:  119 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVTDMGAD 604
            DPYFD+IGCSHPGCEP Y+TPKCV+KCV  N +W+ SKH+SVKAY + SDPQDIM EVYKNGPVEV+FTV+EDF+HYKSGVYKHITG  LGGHAVKLIGWGTSD+GEDYWL ANQWN NWGDDGYFKIKRGTNECGIE  VTAGLP TKN ++ EVTDM  D
Sbjct:  194 DPYFDQIGCSHPGCEPAYQTPKCVRKCVKGNQIWKRSKHYSVKAYRVKSDPQDIMTEVYKNGPVEVAFTVFEDFAHYKSGVYKHITGSALGGHAVKLIGWGTSDEGEDYWLLANQWNTNWGDDGYFKIKRGTNECGIEDDVTAGLPSTKN-IVREVTDMDVD 354

[Close]

BLAST of FE671472 vs. TrEMBL
Match: D7KB70_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_887367 PE=4 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 5.224e-74
Identity = 125/157 (79.62%), Postives = 138/157 (87.90%), Query Frame = -3

Query:  134 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVT 604
            DPYFD  GCSHPGCEP Y TPKCV+KCV+EN LW ESKH+ V AY +N DPQDIMAEVYKNGPVEV+FTVYEDF+HYKSGVYKHITG ++GGHAVKLIGWGTSDDGEDYWL ANQWNR+WGDDGYFKI+RGTNECGIE  V AGLP  +N V ++VT
Sbjct:  194 DPYFDNTGCSHPGCEPGYPTPKCVRKCVSENQLWGESKHYGVSAYRINHDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKHITGTKIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEHGVVAGLPSDRN-VFKDVT 349

[Close]

BLAST of FE671472 vs. TrEMBL
Match: B9GRU7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_552399 PE=3 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 5.224e-74
Identity = 122/157 (77.71%), Postives = 139/157 (88.54%), Query Frame = -3

Query:  134 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIEEVT 604
            DPYFD+IGCSHPGCEP Y TPKC +KCVN+N LW++SKH+ VK Y ++SDP+ IMAE+YKNGPVEV+FTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTS+DGE YWL ANQWNR WGDDGYFKI+RGTNECGIEG V AGLP T+NLV E V+
Sbjct:  192 DPYFDDIGCSHPGCEPGYPTPKCARKCVNKNQLWKKSKHYGVKPYRIDSDPESIMAEIYKNGPVEVAFTVYEDFAHYKSGVYKHITGGMMGGHAVKLIGWGTSEDGEAYWLLANQWNRGWGDDGYFKIRRGTNECGIEGDVVAGLPSTRNLVREVVS 348

[Close]

BLAST of FE671472 vs. TrEMBL
Match: B9RN00_RICCO (Cathepsin B, putative OS=Ricinus communis GN=RCOM_1341910 PE=3 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 1.985e-73
Identity = 122/162 (75.31%), Postives = 139/162 (85.80%), Query Frame = -3

Query:  122 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL-VIEEVTDMGA 604
            DPYFD IGCSHPGCEP + TPKCV+KC+++N LW +SKH+SV AY ++SDP D+MAEVYKNGPVEVSFTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTSD+GEDYWL ANQWNR WGDDGYFKI+RGTNECGIE    AGLP  +NL ++ EV  M A
Sbjct:  209 DPYFDNIGCSHPGCEPGFPTPKCVRKCIDKNQLWRQSKHYSVNAYRISSDPHDVMAEVYKNGPVEVSFTVYEDFAHYKSGVYKHITGEVMGGHAVKLIGWGTSDNGEDYWLLANQWNRGWGDDGYFKIRRGTNECGIEDDAVAGLPSARNLDLVREVASMDA 370

[Close]

BLAST of FE671472 vs. TrEMBL
Match: Q56XY7_ARATH (Cathepsin B-like cysteine proteinase like protein OS=Arabidopsis thaliana GN=At1g02300 PE=2 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 9.852e-73
Identity = 121/151 (80.13%), Postives = 133/151 (88.08%), Query Frame = -3

Query:  152 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL 604
            DPYFD  GCSHPGCEPTY TPKC +KCV+ N LW ESKH+ V AY +N DPQDIMAEVYKNGPVEV+FTVYEDF+HYKSGVYK+ITG ++GGHAVKLIGWGTSDDGEDYWL ANQWNR+WGDDGYFKI+RGTNECGIE  V AGLP  KN+
Sbjct:   18 DPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNV 168

[Close]

BLAST of FE671472 vs. TrEMBL
Match: O23681_ARATH (Cathepsin B-like cysteine proteinase OS=Arabidopsis thaliana GN=At1g02300 PE=3 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 9.852e-73
Identity = 121/151 (80.13%), Postives = 133/151 (88.08%), Query Frame = -3

Query:  152 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL 604
            DPYFD  GCSHPGCEPTY TPKC +KCV+ N LW ESKH+ V AY +N DPQDIMAEVYKNGPVEV+FTVYEDF+HYKSGVYK+ITG ++GGHAVKLIGWGTSDDGEDYWL ANQWNR+WGDDGYFKI+RGTNECGIE  V AGLP  KN+
Sbjct:  192 DPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNV 342

[Close]

BLAST of FE671472 vs. TrEMBL
Match: B9I982_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1098794 PE=3 SV=1)

HSP 1 Score: 275.789 bits (704), Expect = 1.681e-72
Identity = 122/154 (79.22%), Postives = 134/154 (87.01%), Query Frame = -3

Query:  143 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLVIE 604
            DPYFD+IGCSHPGCEP + TPKC +KC ++N LW ESKHFSV AY ++SDP  IMAEV  NGPVEV+FTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTS+DGEDYWL ANQWNR WGDDGYFKIKRGTNECGIEG V AGLP T+NLV E
Sbjct:  174 DPYFDDIGCSHPGCEPGFPTPKCERKCADKNKLWAESKHFSVNAYRIDSDPHSIMAEVSSNGPVEVAFTVYEDFAHYKSGVYKHITGDAMGGHAVKLIGWGTSEDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGIEGAVVAGLPSTRNLVRE 327

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT1G02300.1 (| Symbols: | Cysteine proteinases superfamily protein | chr1:453288-455376 FORWARD LENGTH=379)

HSP 1 Score: 276.559 bits (706), Expect = 7.322e-75
Identity = 121/151 (80.13%), Postives = 133/151 (88.08%), Query Frame = -3

Query:  152 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL 604
            DPYFD  GCSHPGCEPTY TPKC +KCV+ N LW ESKH+ V AY +N DPQDIMAEVYKNGPVEV+FTVYEDF+HYKSGVYK+ITG ++GGHAVKLIGWGTSDDGEDYWL ANQWNR+WGDDGYFKI+RGTNECGIE  V AGLP  KN+
Sbjct:  214 DPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNV 364

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT1G02305.1 (| Symbols: | Cysteine proteinases superfamily protein | chr1:455816-457974 FORWARD LENGTH=362)

HSP 1 Score: 272.707 bits (696), Expect = 1.057e-73
Identity = 119/152 (78.29%), Postives = 130/152 (85.53%), Query Frame = -3

Query:  149 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNLV 604
            DPYFD  GCSHPGCEP Y TPKC +KCV+ N LW ESKH+ V AY + S P DIMAEVYKNGPVEV+FTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTSDDGEDYWL ANQWNR+WGDDGYFKI+RGTNECGIE  V AGLP  +N+V
Sbjct:  197 DPYFDNTGCSHPGCEPAYPTPKCARKCVSGNQLWRESKHYGVSAYKVRSHPDDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKHITGTNIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEHGVVAGLPSDRNVV 348

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT4G01610.2 (| Symbols: | Cysteine proteinases superfamily protein | chr4:694857-696937 FORWARD LENGTH=359)

HSP 1 Score: 272.322 bits (695), Expect = 1.381e-73
Identity = 118/151 (78.15%), Postives = 130/151 (86.09%), Query Frame = -3

Query:  152 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL 604
            DPYFD  GCSHPGCEP Y TPKC +KCV++N LW ESKH+SV  YT+ S+PQDIMAEVYKNGPVEVSFTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTS +GEDYWL ANQWNR WGDDGYF I+RGTNECGIE    AGLP +KN+
Sbjct:  194 DPYFDNTGCSHPGCEPAYPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGIEDEPVAGLPSSKNV 344

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT4G01610.1 (| Symbols: | Cysteine proteinases superfamily protein | chr4:694857-696937 FORWARD LENGTH=359)

HSP 1 Score: 272.322 bits (695), Expect = 1.381e-73
Identity = 118/151 (78.15%), Postives = 130/151 (86.09%), Query Frame = -3

Query:  152 DPYFDEIGCSHPGCEPTYRTPKCVKKCVNENLLWEESKHFSVKAYTLNSDPQDIMAEVYKNGPVEVSFTVYEDFSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGIEGHVTAGLPFTKNL 604
            DPYFD  GCSHPGCEP Y TPKC +KCV++N LW ESKH+SV  YT+ S+PQDIMAEVYKNGPVEVSFTVYEDF+HYKSGVYKHITG  +GGHAVKLIGWGTS +GEDYWL ANQWNR WGDDGYF I+RGTNECGIE    AGLP +KN+
Sbjct:  194 DPYFDNTGCSHPGCEPAYPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGIEDEPVAGLPSSKNV 344

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT5G60360.1 (| Symbols: SAG2, AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=358)

HSP 1 Score: 73.1738 bits (178), Expect = 1.229e-13
Identity = 36/78 (46.15%), Postives = 45/78 (57.69%), Query Frame = -3

Query:  194 PVEVSFTVYEDFSHYKSGVY--KHITGYELG-GHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGI 418
            PV ++F V   F  YKSGVY   H     +   HAV  +G+G  +DG  YWL  N W  +WGD GYFK++ G N CGI
Sbjct:  272 PVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHAVLAVGYGV-EDGVPYWLIKNSWGADWGDKGYFKMEMGKNMCGI 348

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT5G60360.3 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282157 FORWARD LENGTH=361)

HSP 1 Score: 71.633 bits (174), Expect = 3.577e-13
Identity = 35/77 (45.45%), Postives = 44/77 (57.14%), Query Frame = -3

Query:  197 PVEVSFTVYEDFSHYKSGVY--KHITGYELG-GHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECG 418
            PV ++F V   F  YKSGVY   H     +   HAV  +G+G  +DG  YWL  N W  +WGD GYFK++ G N CG
Sbjct:  272 PVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHAVLAVGYGV-EDGVPYWLIKNSWGADWGDKGYFKMEMGKNMCG 347

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT3G45310.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=358)

HSP 1 Score: 70.4774 bits (171), Expect = 7.969e-13
Identity = 34/78 (43.59%), Postives = 43/78 (55.13%), Query Frame = -3

Query:  194 PVEVSFTVYEDFSHYKSGVYKHITGYELG---GHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNECGI 418
            PV V+F V  +F  YK GV+   T         HAV  +G+G  DD   YWL  N W   WGD+GYFK++ G N CG+
Sbjct:  272 PVSVAFEVVHEFRFYKKGVFTSNTCGNTPMDVNHAVLAVGYGVEDD-VPYWLIKNSWGGEWGDNGYFKMEMGKNMCGV 348

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT2G34080.1 (| Symbols: | Cysteine proteinases superfamily protein | chr2:14393431-14394777 REVERSE LENGTH=345)

HSP 1 Score: 70.0922 bits (170), Expect = 1.041e-12
Identity = 35/86 (40.70%), Postives = 44/86 (51.16%), Query Frame = -3

Query:  215 TLNSDPQDIMAEVYKNGPVEVSFTVYED-FSHYKSGVYKHITGYELGGHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKR 469
            T+ S+ +  + E     PV VS     D F HY  GVY    G     HAV  +G+GTS DG  YWL  N W   WG+ GY +I+R
Sbjct:  241 TVPSNNERALLEAVSRQPVSVSMDATGDGFMHYSGGVYDGPCGTS-SNHAVTFVGYGTSQDGTKYWLAKNSWGETWGEKGYIRIRR 325

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT5G60360.2 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=357)

HSP 1 Score: 69.3218 bits (168), Expect = 1.775e-12
Identity = 34/76 (44.74%), Postives = 43/76 (56.58%), Query Frame = -3

Query:  200 PVEVSFTVYEDFSHYKSGVY--KHITGYELG-GHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNEC 418
            PV ++F V   F  YKSGVY   H     +   HAV  +G+G  +DG  YWL  N W  +WGD GYFK++ G N C
Sbjct:  272 PVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHAVLAVGYGV-EDGVPYWLIKNSWGADWGDKGYFKMEMGKNMC 346

[Close]

BLAST of FE671472 vs. TAIR peptide
Match: AT3G45310.2 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=357)

HSP 1 Score: 67.0106 bits (162), Expect = 8.811e-12
Identity = 33/76 (43.42%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  200 PVEVSFTVYEDFSHYKSGVYKHITGYELG---GHAVKLIGWGTSDDGEDYWLFANQWNRNWGDDGYFKIKRGTNEC 418
            PV V+F V  +F  YK GV+   T         HAV  +G+G  DD   YWL  N W   WGD+GYFK++ G N C
Sbjct:  272 PVSVAFEVVHEFRFYKKGVFTSNTCGNTPMDVNHAVLAVGYGVEDD-VPYWLIKNSWGGEWGDNGYFKMEMGKNMC 346

The following BLAST results are available for this feature:

BLAST of FE671472 vs. SwissProt
BLAST of FE671472 vs. TrEMBL
BLAST of FE671472 vs. TAIR peptide

BLAST of FE671472 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CPR4_CAEEL	1.746e-40	55.07	Cathepsin B-like cysteine proteinase 4 OS=Caenorha...	[more]
CATB_PIG	2.521e-39	48.65	Cathepsin B OS=Sus scrofa GN=CTSB PE=1 SV=1	[more]
CATB_PONAB	3.292e-39	48.65	Cathepsin B OS=Pongo abelii GN=CTSB PE=2 SV=1	[more]
CATB_MACFA	3.292e-39	48.65	Cathepsin B OS=Macaca fascicularis GN=CTSB PE=2 SV...	[more]
CATB_HUMAN	3.292e-39	48.65	Cathepsin B OS=Homo sapiens GN=CTSB PE=1 SV=3	[more]
CATB_RAT	1.251e-38	48.32	Cathepsin B OS=Rattus norvegicus GN=Ctsb PE=1 SV=2	[more]
CATB_MOUSE	1.634e-38	48.65	Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2	[more]
CATB_BOVIN	2.787e-38	47.30	Cathepsin B OS=Bos taurus GN=CTSB PE=1 SV=5	[more]
CPR5_CAEEL	6.865e-37	51.05	Cathepsin B-like cysteine proteinase 5 OS=Caenorha...	[more]
CPR1_CAEEL	1.171e-36	51.82	Gut-specific cysteine proteinase OS=Caenorhabditis...	[more]

BLAST of FE671472 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2HV10_MEDTR	1.791e-82	85.19	Peptidase C1A, papain; Somatotropin hormone; Pepti...	[more]
Q2HV09_MEDTR	2.677e-78	80.86	Peptidase C1A, papain; Somatotropin hormone; Pepti...	[more]
B7FJ05_MEDTR	2.677e-78	80.86	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FK90_MEDTR	7.788e-78	80.25	Putative uncharacterized protein OS=Medicago trunc...	[more]
D7KB70_ARALY	5.224e-74	79.62	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9GRU7_POPTR	5.224e-74	77.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RN00_RICCO	1.985e-73	75.31	Cathepsin B, putative OS=Ricinus communis GN=RCOM_...	[more]
Q56XY7_ARATH	9.852e-73	80.13	Cathepsin B-like cysteine proteinase like protein ...	[more]
O23681_ARATH	9.852e-73	80.13	Cathepsin B-like cysteine proteinase OS=Arabidopsi...	[more]
B9I982_POPTR	1.681e-72	79.22	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of FE671472 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G02300.1	7.322e-75	80.13	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT1G02305.1	1.057e-73	78.29	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT4G01610.2	1.381e-73	78.15	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT4G01610.1	1.381e-73	78.15	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT5G60360.1	1.229e-13	46.15	\| Symbols: SAG2, AALP, ALP \| aleurain-like proteas...	[more]
AT5G60360.3	3.577e-13	45.45	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT3G45310.1	7.969e-13	43.59	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT2G34080.1	1.041e-12	40.70	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT5G60360.2	1.775e-12	44.74	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT3G45310.2	8.811e-12	43.42	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 91..101 score
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 21..143 score: 1
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	no description	coord: 1..145 score: 4.2
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 1..151 score: 5.7
IPR015643	Peptidase C1A, cathepsin B	PANTHER	PTHR12411:SF16	CATHEPSIN B	coord: 1..151 score: 5.7
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 11..146 score: 8.2
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 14..156 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671472 ID=FE671472; Name=FE671472; organism=Cicer arietinum; type=EST; length=606bp
AGCCAAGTTACGTGCATTGATAAAATAAAGATATTTACATCTCAGTATTT
GGATTGATATATTGCCAACACAAAATCTAACTGTTAAGGTAATACTATAT
TTACATTCATGATGAAAAATCAGCACCCATGTCAGTCACCTCTTCTATTA
CAAGATTTTTGGTAAAAGGCAAACCAGCTGTAACATGACCTTCAATCCCA
CATTCGTTTGTCCCTCTCTTGATTTTGAAGTAACCATCATCTCCCCAATT
TCTATTCCACTGATTTGCAAAAAGCCAATAGTCCTCCCCATCATCACTTG
TTCCCCATCCAATCAGTTTTACTGCATGACCGCCTAGTTCGTAACCTGTG
ATGTGTTTATAAACTCCTGATTTGTAGTGAGAAAAATCCTCATAAACAGT
GAATGAAACTTCAACTGGCCCGTTCTTATAAACTTCTGCCATAATATCTT
GCGGATCAGAGTTTAGCGTATATGCTTTGACACTGAAGTGCTTTGATTCC
TCCCAAAGCAGGTTCTCATTTACACACTTTTTAACACACTTGGGAGTTCG
ATATGTTGGTTCACAACCAGGATGGGAACATCCAATTTCATCAAAGTATG
GATCAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004197	cysteine-type endopeptidase activity
GO:0008234	cysteine-type peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A
IPR015643	Peptidase_C1A_cathepsin-B

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