GR390737

EST Overview


Name	GR390737
Unique Name	GR390737
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	360

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL20_YEAST (60S ribosomal protein L20 OS=Saccharomyces cerevisiae GN=RPL20A PE=1 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 5.601e-22
Identity = 51/76 (67.11%), Postives = 59/76 (77.63%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            SR  AVE+LY DMAARHRARFRSIHIL+V EIEKT D+KR Y++Q   K L FPLPHR+ K  + + FS KRPSTF
Sbjct:   98 SRVAAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQK--STKTFSYKRPSTF 171

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL20_SCHPO (60S ribosomal protein L20 OS=Schizosaccharomyces pombe GN=rpl20a PE=1 SV=3)

HSP 1 Score: 92.4337 bits (228), Expect = 7.570e-19
Identity = 45/79 (56.96%), Postives = 59/79 (74.68%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHR---ITKENTKRIFSAKRPSTF 234
            +R  AVE++Y+DMAARHRARFRSI IL+VVE+EK ED++R Y++QL    L FPLPHR   +     K++F+  RPSTF
Sbjct:   97 TRVGAVEAMYADMAARHRARFRSIRILKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVVGLAGKKVFAPHRPSTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_SALSA (60S ribosomal protein L18a OS=Salmo salar GN=rpl18a PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.997e-11
Identity = 34/72 (47.22%), Postives = 44/72 (61.11%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SIHI++V EI   +  +RP I+Q     + FPLPHR+ +      F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHSIHIMKVQEIAANK-CRRPAIKQFHDSKIKFPLPHRVLRRQHNPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_HUMAN (60S ribosomal protein L18a OS=Homo sapiens GN=RPL18A PE=1 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 4.448e-11
Identity = 33/72 (45.83%), Postives = 45/72 (62.50%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SI I++V EI  ++  +RP ++Q     + FPLPHR+ +   K  F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHSIQIMKVEEIAASK-CRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_BOVIN (60S ribosomal protein L18a OS=Bos taurus GN=RPL18A PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.448e-11
Identity = 33/72 (45.83%), Postives = 45/72 (62.50%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SI I++V EI  ++  +RP ++Q     + FPLPHR+ +   K  F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHSIQIMKVEEIAASK-CRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_RAT (60S ribosomal protein L18a OS=Rattus norvegicus GN=Rpl18a PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.910e-11
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SI I++V EI   +  +RP ++Q     + FPLPHR+ +   K  F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHSIQIMKVEEIAAGK-CRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_MOUSE (60S ribosomal protein L18a OS=Mus musculus GN=Rpl18a PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.910e-11
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SI I++V EI   +  +RP ++Q     + FPLPHR+ +   K  F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHSIQIMKVEEIAAGK-CRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_DANRE (60S ribosomal protein L18a OS=Danio rerio GN=rpl18a PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.918e-10
Identity = 31/72 (43.06%), Postives = 43/72 (59.72%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  +I I++V ++      +RP I+Q     + FPLPHR+ +   K  F+ KRP+TF
Sbjct:  105 AVTQCYRDMGARHRARAHAIQIMKV-QVIAANKCRRPAIKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTF 175

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_CAEEL (60S ribosomal protein L18a OS=Caenorhabditis elegans GN=rpl-20 PE=2 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 1.431e-9
Identity = 32/71 (45.07%), Postives = 41/71 (57.75%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPST 231
            AV   Y DM ARHRA+   IHIL+V  + K ED KR  I+      + FPLPHR+TK     +F+  R +T
Sbjct:  108 AVTQCYRDMGARHRAQADRIHILKVQTV-KAEDTKRAGIKMFHDAKIRFPLPHRVTKRKNLSVFTTARQNT 177

[Close]

BLAST of GR390737 vs. SwissProt
Match: RL18A_SPOFR (60S ribosomal protein L18a OS=Spodoptera frugiperda GN=RpL18A PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.188e-9
Identity = 31/72 (43.06%), Postives = 41/72 (56.94%), Query Frame = 1

Query:   19 AVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            AV   Y DM ARHRAR  SI I++V E+ K    +RP ++Q     + FPLP R+        F+ KRPST+
Sbjct:  105 AVTQCYRDMGARHRARAHSIQIIKV-EVIKASACRRPQVKQFHNSTIRFPLPKRVHHHKRLNTFAYKRPSTY 175

[Close]

BLAST of GR390737 vs. TrEMBL
Match: C7YWB0_NECH7 (Predicted protein OS=Nectria haematococca (strain 77-13-4 / FGSC 9596 / MPVI) GN=NECHADRAFT_65202 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 5.867e-33
Identity = 69/77 (89.61%), Postives = 75/77 (97.40%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRTDAV+SLYSDMAARHRARFRSIHILRVVE+EKTED+KRPYIRQLTQKGLSFPLPHRI KE+ K++FSAKRPSTFA
Sbjct:  100 SRTDAVDSLYSDMAARHRARFRSIHILRVVELEKTEDVKRPYIRQLTQKGLSFPLPHRIPKESAKKVFSAKRPSTFA 176

[Close]

BLAST of GR390737 vs. TrEMBL
Match: B2AAF1_PODAN (Predicted CDS Pa_1_3790 OS=Podospora anserina PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 1.495e-28
Identity = 62/77 (80.52%), Postives = 69/77 (89.61%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRT AVE+LYSDMAARHRARFRSIHILRVVEIEKTED+KRPYI+QL  K L+FPLPHR+ K NTK++FSA RPST A
Sbjct:   72 SRTKAVEALYSDMAARHRARFRSIHILRVVEIEKTEDVKRPYIKQLITKDLTFPLPHRVAKTNTKKVFSATRPSTIA 148

[Close]

BLAST of GR390737 vs. TrEMBL
Match: Q5G576_MAGGR (60S ribosomal protein L20-like protein OS=Magnaporthe grisea GN=MGG_03653 PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.551e-28
Identity = 61/77 (79.22%), Postives = 70/77 (90.91%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRT AVE++Y DMAARHRARF SIHILRVVE+EKT+DIKRPYI+QLT KGL+FPLPHR+ K N K+IFSA+RPSTFA
Sbjct:   98 SRTAAVEAMYQDMAARHRARFASIHILRVVELEKTDDIKRPYIKQLTTKGLTFPLPHRVPKINNKKIFSARRPSTFA 174

[Close]

BLAST of GR390737 vs. TrEMBL
Match: Q7SD62_NEUCR (60S ribosomal protein L20 OS=Neurospora crassa GN=NCU08389 PE=4 SV=2)

HSP 1 Score: 126.331 bits (316), Expect = 9.693e-28
Identity = 61/77 (79.22%), Postives = 70/77 (90.91%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRT AVE+LYSDMAARHRARFRSIHIL+VVE+EKTEDIKRPY++QL  K LSFPLPHR+ K + K+IFSAKRP+TFA
Sbjct:   98 SRTAAVEALYSDMAARHRARFRSIHILKVVELEKTEDIKRPYLKQLVTKKLSFPLPHRVPKISNKKIFSAKRPATFA 174

[Close]

BLAST of GR390737 vs. TrEMBL
Match: Q2HD87_CHAGB (Putative uncharacterized protein OS=Chaetomium globosum GN=CHGG_01817 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.266e-27
Identity = 61/77 (79.22%), Postives = 69/77 (89.61%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRT AVE+LYSDMAARHRARFRSIHILRVVEIEKTED+KRPY++QL  K LSFPLPHR+ + +  +IFSAKRPSTFA
Sbjct:   98 SRTAAVEALYSDMAARHRARFRSIHILRVVEIEKTEDVKRPYLKQLLTKDLSFPLPHRVPRISNSKIFSAKRPSTFA 174

[Close]

BLAST of GR390737 vs. TrEMBL
Match: D4APJ1_ARTBC (Putative uncharacterized protein OS=Arthroderma benhamiae (strain CBS 112371) GN=ARB_06159 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.266e-27
Identity = 59/77 (76.62%), Postives = 67/77 (87.01%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRTDAVES+Y DMAARHRARFRSIHIL+VVEI+KTE I+RPYI+QL  KGL FPLPHR+ K   K++FSA RPSTFA
Sbjct:   72 SRTDAVESMYQDMAARHRARFRSIHILKVVEIDKTESIRRPYIKQLLSKGLKFPLPHRVAKPTNKKLFSASRPSTFA 148

[Close]

BLAST of GR390737 vs. TrEMBL
Match: D1ZQW4_SORMA (Whole genome shotgun sequence assembly, scaffold_81 OS=Sordaria macrospora GN=SMAC_04642 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.266e-27
Identity = 61/77 (79.22%), Postives = 70/77 (90.91%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRT AVE+LYSDMAARHRARFRSIHIL+VVE+EKTEDIKRPYI+QL  K LSFPLPHR+ + + K+IFSAKRP+TFA
Sbjct:   98 SRTAAVEALYSDMAARHRARFRSIHILKVVELEKTEDIKRPYIKQLVTKKLSFPLPHRVPRISNKKIFSAKRPATFA 174

[Close]

BLAST of GR390737 vs. TrEMBL
Match: D4D9J9_TRIVH (Putative uncharacterized protein OS=Trichophyton verrucosum (strain HKI 0517) GN=TRV_03792 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.820e-27
Identity = 58/77 (75.32%), Postives = 67/77 (87.01%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTFA 237
            SRTDAVES+Y DMAARHRARFRSIHIL+VVEI+KTE I+RPY++QL  KGL FPLPHR+ K   K++FSA RPSTFA
Sbjct:  117 SRTDAVESMYQDMAARHRARFRSIHILKVVEIDKTESIRRPYMKQLLSKGLKFPLPHRVAKPTNKKLFSASRPSTFA 193

[Close]

BLAST of GR390737 vs. TrEMBL
Match: A6SI86_BOTFB (60S ribosomal protein L18 OS=Botryotinia fuckeliana (strain B05.10) GN=BC1G_12468 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.810e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            SRTDAVE+LY DMAARHR+RFRSIHIL+VVEIEKTED+KRPYI+QL +K L FPLPHRI+K  T  +F++KRPSTF
Sbjct:   98 SRTDAVEALYQDMAARHRSRFRSIHILKVVEIEKTEDVKRPYIKQLLEKDLKFPLPHRISKSMTANLFASKRPSTF 173

[Close]

BLAST of GR390737 vs. TrEMBL
Match: D5G9W2_9PEZI (Whole genome shotgun sequence assembly, scaffold_17, strain Mel28 OS=Tuber melanosporum GN=GSTUM_00005099001 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.283e-27
Identity = 58/76 (76.32%), Postives = 66/76 (86.84%), Query Frame = 1

Query:    7 SRTDAVESLYSDMAARHRARFRSIHILRVVEIEKTEDIKRPYIRQLTQKGLSFPLPHRITKENTKRIFSAKRPSTF 234
            SRTDAV+SLY DMAARHRARFRSIHIL+VVEIEK  D+KRPY+RQL  KGL FPLPHR+ K  + ++FSAKRPSTF
Sbjct:   72 SRTDAVQSLYQDMAARHRARFRSIHILKVVEIEKASDVKRPYLRQLITKGLKFPLPHRVPKSVSAKVFSAKRPSTF 147

The following BLAST results are available for this feature:

BLAST of GR390737 vs. SwissProt
BLAST of GR390737 vs. TrEMBL

BLAST of GR390737 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RL20_YEAST	5.601e-22	67.11	60S ribosomal protein L20 OS=Saccharomyces cerevis...	[more]
RL20_SCHPO	7.570e-19	56.96	60S ribosomal protein L20 OS=Schizosaccharomyces p...	[more]
RL18A_SALSA	1.997e-11	47.22	60S ribosomal protein L18a OS=Salmo salar GN=rpl18...	[more]
RL18A_HUMAN	4.448e-11	45.83	60S ribosomal protein L18a OS=Homo sapiens GN=RPL1...	[more]
RL18A_BOVIN	4.448e-11	45.83	60S ribosomal protein L18a OS=Bos taurus GN=RPL18A...	[more]
RL18A_RAT	9.910e-11	45.83	60S ribosomal protein L18a OS=Rattus norvegicus GN...	[more]
RL18A_MOUSE	9.910e-11	45.83	60S ribosomal protein L18a OS=Mus musculus GN=Rpl1...	[more]
RL18A_DANRE	4.918e-10	43.06	60S ribosomal protein L18a OS=Danio rerio GN=rpl18...	[more]
RL18A_CAEEL	1.431e-9	45.07	60S ribosomal protein L18a OS=Caenorhabditis elega...	[more]
RL18A_SPOFR	3.188e-9	43.06	60S ribosomal protein L18a OS=Spodoptera frugiperd...	[more]

BLAST of GR390737 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C7YWB0_NECH7	5.867e-33	89.61	Predicted protein OS=Nectria haematococca (strain ...	[more]
B2AAF1_PODAN	1.495e-28	80.52	Predicted CDS Pa_1_3790 OS=Podospora anserina PE=4...	[more]
Q5G576_MAGGR	2.551e-28	79.22	60S ribosomal protein L20-like protein OS=Magnapor...	[more]
Q7SD62_NEUCR	9.693e-28	79.22	60S ribosomal protein L20 OS=Neurospora crassa GN=...	[more]
Q2HD87_CHAGB	1.266e-27	79.22	Putative uncharacterized protein OS=Chaetomium glo...	[more]
D4APJ1_ARTBC	1.266e-27	76.62	Putative uncharacterized protein OS=Arthroderma be...	[more]
D1ZQW4_SORMA	1.266e-27	79.22	Whole genome shotgun sequence assembly, scaffold_8...	[more]
D4D9J9_TRIVH	2.820e-27	75.32	Putative uncharacterized protein OS=Trichophyton v...	[more]
A6SI86_BOTFB	4.810e-27	76.32	60S ribosomal protein L18 OS=Botryotinia fuckelian...	[more]
D5G9W2_9PEZI	6.283e-27	76.32	Whole genome shotgun sequence assembly, scaffold_1...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002670	Ribosomal protein L18ae	PANTHER	PTHR10052:SF1	60S RIBOSOMAL PROTEIN L18A	coord: 3..79 score: 1.3
IPR021138	Ribosomal protein L18ae/LX	PFAM	PF01775	Ribosomal_L18ae	coord: 3..31 score: 1.2
None	No IPR available	GENE3D	3.10.20.10	no description	coord: 4..49 score: 5.6
None	No IPR available	PANTHER	PTHR10052	60S RIBOSOMAL PROTEIN L18A	coord: 3..79 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR390737 ID=GR390737; Name=GR390737; organism=Cicer arietinum; type=EST; length=360bp
GCGGGGTCCCGAACAGATGCTGTCGAGTCTCTCTACTCCGACATGGCTGC
CCGCCACCGTGCTCGTTTCAGGTCCATCCACATCCTCCGCGTTGTCGAGA
TTGAGAAGACCGAGGACATCAAGCGACCCTACATCCGCCAGCTGACCCAG
AAGGGACTCAGCTTCCCTCTCCCCCACCGCATCACCAAGGAGAACACCAA
GAGGATCTTCAGCGCGAAGCGACCTTCCACTTTCGCTTAGAATGATTTCG
GTTTAGAGGACGGTTGGGCTCTGCGGGTGTTACAACGGATCATGGAAATA
TCAACTGACTTTGCTCAATAAAACGGGCATTGAATGAACCAAAAATTCTG
AGCATGAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR002670	Ribosomal protein L18ae
IPR021138	Ribosomal_eL20_eukaryotes

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