GR399356

EST Overview


Name	GR399356
Unique Name	GR399356
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	677

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR399356 vs. SwissProt
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 190.66 bits (483), Expect = 6.254e-48
Identity = 83/147 (56.46%), Postives = 114/147 (77.55%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIE V + +     A    +CS C+M V+W++NQL++  TKE + NY NQLCE LPSP+GES +SC+ +S+MPN++FTI +K F+LTPEQY+++  +G   VC+S F+AFDIPPP+GPLWILGDVFM AYHTVFD+G  ++GFA++A
Sbjct:  354 GIESVVDKENLGSDA----MCSVCEMAVVWIENQLRENKTKELILNYANQLCERLPSPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496

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BLAST of GR399356 vs. SwissProt
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 190.274 bits (482), Expect = 8.168e-48
Identity = 83/148 (56.08%), Postives = 111/148 (75.00%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGT-PLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GI+ V +++  E +   + P+C++C+M V+W+QNQL Q  T++ + NY+NQLC+ LPSP GES + C  L+ MP ISFTIG K F L PE+Y+L+ GEG    C+S F A DIPPP+GPLWILGDVFM AYHTVFDYG ++VGFA++A
Sbjct:  362 GIKSVVDDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509

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BLAST of GR399356 vs. SwissProt
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.393e-47
Identity = 86/148 (58.11%), Postives = 108/148 (72.97%), Query Frame = 2

Query:    2 GIE-MVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIE +V EN      +    +CS C+M V+W+QNQL+Q  TKER+ NY+N+LC+ +PSP G+S + C  LS MP +SFTIG K F L PE+Y+L+ GEG    C+S F AFDIPPP+GPLWILGDVFM  YHTVFD+G L+VG AEAA
Sbjct:  366 GIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513

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BLAST of GR399356 vs. SwissProt
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 186.422 bits (472), Expect = 1.180e-46
Identity = 87/147 (59.18%), Postives = 108/147 (73.47%), Query Frame = 2

Query:    5 IEMVTENKQSEMSATGTPL-CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            IE V +  + + S  G P+ C  C   V+W+QNQ++Q  T+E + NYV++LCE LPSP GES + C+ LS MPNI+FT+G K F L+PEQYVL+ GEG T  C+S F A D+ PP GPLWILGDVFM  YHTVFDYGNL+VGFAEAA
Sbjct:  329 IESVVDKSKGKSS--GLPMRCVPCARWVVWMQNQIRQNETEENIINYVDKLCERLPSPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473

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BLAST of GR399356 vs. SwissProt
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 3.432e-46
Identity = 82/148 (55.41%), Postives = 106/148 (71.62%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSAT-GTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GI  V +++  + +     P+CS+C+M V+W+QNQL Q  T++ + +YVNQLC  LPSP GES + C  L  MP+I FTIG K F L PE+Y+L+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFDYG L++GFA+AA
Sbjct:  361 GIRSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

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BLAST of GR399356 vs. SwissProt
Match: CARDB_CYNCA (Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 9.671e-41
Identity = 73/145 (50.34%), Postives = 104/145 (71.72%), Query Frame = 2

Query:    5 IEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            IE V +    + S  G  +C+ C+M ++ +QN++K+  T++ + N+VN++C+ LP+ S ES++ CN +S MPNI+FTIG K F +TPEQY+ + GEG    C+S F A DI  P+GP+WILGD+FM  YHTVFDYG L+VGFAEA
Sbjct:  363 IESVVDKNNDKSS--GGEICTFCEMALVRMQNEIKRNETEDNIINHVNEVCDQLPTSSAESIVDCNGISSMPNIAFTIGSKLFEVTPEQYIYKVGEGEAATCISGFTALDIMSPQGPIWILGDMFMGPYHTVFDYGKLRVGFAEA 505

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BLAST of GR399356 vs. SwissProt
Match: CARDA_CYNCA (Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 9.671e-41
Identity = 78/144 (54.17%), Postives = 98/144 (68.06%), Query Frame = 2

Query:   11 MVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            +V +N           +C+ C+M V+W+QN++KQ  T++ + NY N+LCE L + S E  + CN LS MPN+SFTIG K F LTPEQY+L+ G+G    C+S F A D     GPLWILGDVFMR YHTVFDYGNL VGFAEAA
Sbjct:  362 VVDKNNDKSSGGIHDEMCTFCEMAVVWMQNEIKQSETEDNIINYANELCEHLSTSSEELQVDCNTLSSMPNVSFTIGGKKFGLTPEQYILKVGKGEATQCISGFTAMD-ATLLGPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 504

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BLAST of GR399356 vs. SwissProt
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.926e-21
Identity = 47/94 (50.00%), Postives = 63/94 (67.02%), Query Frame = 2

Query:  161 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            ++P   GE +I C  +S +P I+  +G K + L+PE Y L+  +    +CLS F+  DIPPP GPLWILGDVF+  Y+TVFD  N +VGFAEAA
Sbjct:  317 AVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410

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BLAST of GR399356 vs. SwissProt
Match: CATD_BOVIN (Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 3.821e-21
Identity = 46/94 (48.94%), Postives = 62/94 (65.96%), Query Frame = 2

Query:  161 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            ++P   GE +I C  +S +P ++  +G K + L+PE Y L+  +  T VCLS F+  DIPPP GPLWILGDVF+  Y+TVFD    +VG AEAA
Sbjct:  295 AVPLIQGEYMIPCEKVSSLPEVTVKLGGKDYALSPEDYALKVSQAETTVCLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDQNRVGLAEAA 388

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BLAST of GR399356 vs. SwissProt
Match: CATD_MOUSE (Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 6.517e-21
Identity = 44/93 (47.31%), Postives = 63/93 (67.74%), Query Frame = 2

Query:  161 SLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            ++P   GE +I C  +S +P +   +G K + L P++Y+L+  +G   +CLS F+  DIPPP GPLWILGDVF+ +Y+TVFD  N +VGFA A
Sbjct:  315 AVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFANA 407

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BLAST of GR399356 vs. TrEMBL
Match: Q9SXU0_CICAR (Aspartic proteinase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 8.258e-80
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIEMVTENKQSEMSAT TPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:   58 GIEMVTENKQSEMSATDTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 204

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BLAST of GR399356 vs. TrEMBL
Match: Q2HRQ7_MEDTR (Peptidase A1, pepsin OS=Medicago truncatula GN=MtrDRAFT_AC157894g38v2 PE=3 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 7.999e-75
Identity = 133/147 (90.48%), Postives = 143/147 (97.28%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIEMVT+ +QSE+SA  TPLCSSCQMLV+WVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCND+S+MPNISFTIG+KPFVLTPEQY+LRTGEGIT+VCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA
Sbjct:  250 GIEMVTDKEQSELSAKDTPLCSSCQMLVLWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDISKMPNISFTIGNKPFVLTPEQYILRTGEGITQVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 396

[Close]

BLAST of GR399356 vs. TrEMBL
Match: Q948P0_SOYBN (Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=2 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 5.732e-73
Identity = 129/146 (88.36%), Postives = 140/146 (95.89%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            GIEMVTE +Q E++A  TPLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+LRTGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:  362 GIEMVTEKEQEELAARDTPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 507

[Close]

BLAST of GR399356 vs. TrEMBL
Match: C6TDL8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 4.853e-72
Identity = 127/146 (86.99%), Postives = 139/146 (95.21%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            GIEMVTE +Q E++A   PLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+L+TGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:   31 GIEMVTEKEQGELTARDNPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILKTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 176

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BLAST of GR399356 vs. TrEMBL
Match: C6TIU2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 271.937 bits (694), Expect = 3.145e-71
Identity = 126/143 (88.11%), Postives = 137/143 (95.80%), Query Frame = 2

Query:   11 MVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            MVTE +Q E++A  TPLCSSCQMLV+W+QNQLKQKATK+RVFNYVNQLCESLPSPSGESVISCN LS+MPNI+FTIG+KPFVLTPEQY+LRTGEGITEVCLS FIAFD+PPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA
Sbjct:    1 MVTEKEQEELAARDTPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 143

[Close]

BLAST of GR399356 vs. TrEMBL
Match: Q8H948_SOYBN (Aspartic proteinase 5 (Fragment) OS=Glycine max GN=soyAP5 PE=2 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 1.158e-65
Identity = 115/147 (78.23%), Postives = 131/147 (89.12%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIEMVTE +Q E+S   T LC+SCQMLV+W+QNQLKQK TKE VFNYVNQLCESLPSP+GESV+ CN +  +PNI+FT+GDKPF LTPEQY+L+TGEGI EVCLS FIAFDIPPP+GPLWILGDVFMR YHTVFDYGNL+VGFA+AA
Sbjct:   29 GIEMVTEKEQRELSTKDTALCTSCQMLVVWIQNQLKQKKTKEIVFNYVNQLCESLPSPNGESVVDCNSIYGLPNITFTVGDKPFTLTPEQYILKTGEGIAEVCLSGFIAFDIPPPRGPLWILGDVFMRVYHTVFDYGNLRVGFAKAA 175

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BLAST of GR399356 vs. TrEMBL
Match: B9I6C6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834438 PE=3 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 2.338e-58
Identity = 104/148 (70.27%), Postives = 125/148 (84.46%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPL-CSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIE V E +  E S+ G  L C++CQMLVIWVQNQL++KATKE   NY+++LCESLPSP G+S I CN +S MPNI+FTIGDKPF LTPEQY+L+TGEGI +VC+S F+A D+PPP+GPLWILGDVFM AYHT+FDYGNL+VGFAEAA
Sbjct:  360 GIESVVEKENKEKSSAGNDLPCTACQMLVIWVQNQLREKATKETAINYLDKLCESLPSPMGQSSIDCNSISTMPNITFTIGDKPFSLTPEQYILKTGEGIAQVCISGFMALDVPPPRGPLWILGDVFMGAYHTIFDYGNLEVGFAEAA 507

[Close]

BLAST of GR399356 vs. TrEMBL
Match: Q93XR0_IPOBA (Putative aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 1.160e-57
Identity = 104/146 (71.23%), Postives = 123/146 (84.25%), Query Frame = 2

Query:    5 IEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            I+ V E K++E + T  PLC++C+M VIW+QNQLKQK  KE+VF YV+QLCE LPSP GESVI CN +S MPN++F IGDK FVLTPEQY+L+TGEGI  VC+S F+A D+PPP+GPLWILGDVFM AYHTVFDYGNLQVGFAEAA
Sbjct:  360 IKTVVE-KEAEGNLTSNPLCTTCEMAVIWLQNQLKQKGIKEKVFEYVDQLCEKLPSPDGESVIDCNSISSMPNVTFVIGDKDFVLTPEQYILKTGEGIAAVCVSGFLALDVPPPQGPLWILGDVFMGAYHTVFDYGNLQVGFAEAA 504

[Close]

BLAST of GR399356 vs. TrEMBL
Match: B1Q478_CAPCH (Putative aspartic protease OS=Capsicum chinense PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 4.127e-55
Identity = 98/146 (67.12%), Postives = 115/146 (78.77%), Query Frame = 2

Query:    5 IEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            I  V E +    S    PLC+ C+M V+W+QNQLKQ+ TKERV  YV+QLCE LPSP GESV+ CN +S +PNI+FTI DK FVLTPEQY+L+TGEGI  +C+S F AFD+PPP+GPLWILGDVFM  YHTVFDYGN QVGFAEAA
Sbjct:  361 IRTVVERENEGSSVGEAPLCTVCEMAVVWIQNQLKQQGTKERVLEYVDQLCEKLPSPMGESVVDCNSISSLPNITFTIKDKAFVLTPEQYILKTGEGIASICISGFAAFDVPPPRGPLWILGDVFMGPYHTVFDYGNSQVGFAEAA 506

[Close]

BLAST of GR399356 vs. TrEMBL
Match: Q06EY4_IPOBA (Aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 1.201e-54
Identity = 95/146 (65.07%), Postives = 119/146 (81.51%), Query Frame = 2

Query:    5 IEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            I MV E K +E  ++  P+C++C+M V+W+QNQLKQK  KE+VF+YVNQLCE +PSP GES I CN +S MPN++F I DK FVLTPEQY+L+TGEG+  +C+S F+A D+P P+GPLWILGDVFM  YHTVFDYGNLQ+GFAEAA
Sbjct:  364 IGMVVE-KDNEGKSSSDPMCTACEMAVVWMQNQLKQKVVKEKVFDYVNQLCEKIPSPMGESTIDCNSISNMPNVTFKIADKDFVLTPEQYILKTGEGVATICVSGFLAMDVPAPRGPLWILGDVFMGVYHTVFDYGNLQIGFAEAA 508

[Close]

BLAST of GR399356 vs. TAIR peptide
Match: AT1G11910.1 (| Symbols: APA1, ATAPA1 | aspartic proteinase A1 | chr1:4017119-4019874 REVERSE LENGTH=506)

HSP 1 Score: 192.971 bits (489), Expect = 1.277e-49
Identity = 88/148 (59.46%), Postives = 110/148 (74.32%), Query Frame = 2

Query:    2 GIEMVTENKQSEMS-ATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GIE V + + +++S   G   CS+C+M V+W+Q+QL+Q  T+ER+ NYVN+LCE LPSP GES + C  LS MP +S TIG K F L PE+YVL+ GEG    C+S FIA D+ PP+GPLWILGDVFM  YHTVFD+GN QVGFAEAA
Sbjct:  359 GIESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

[Close]

BLAST of GR399356 vs. TAIR peptide
Match: AT1G62290.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 188.734 bits (478), Expect = 2.409e-48
Identity = 86/148 (58.11%), Postives = 113/148 (76.35%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGT--PLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            GIE V + K++  S++G     C +C+M V+W+Q+QL+Q  T+ER+ NY+N++CE +PSP+GES + C+ LS+MP +SFTIG K F L PE+YVL+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  366 GIESVVD-KENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of GR399356 vs. TAIR peptide
Match: AT1G62290.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 188.734 bits (478), Expect = 2.409e-48
Identity = 86/148 (58.11%), Postives = 113/148 (76.35%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGT--PLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 439
            GIE V + K++  S++G     C +C+M V+W+Q+QL+Q  T+ER+ NY+N++CE +PSP+GES + C+ LS+MP +SFTIG K F L PE+YVL+ GEG    C+S F A DIPPP+GPLWILGDVFM  YHTVFD+GN QVGFAEA
Sbjct:  366 GIESVVD-KENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

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BLAST of GR399356 vs. TAIR peptide
Match: AT4G04460.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=504)

HSP 1 Score: 153.295 bits (386), Expect = 1.122e-37
Identity = 66/147 (44.90%), Postives = 100/147 (68.03%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GI+ V ++  S +      +CS+C+M  +W++++L Q  T+ER+  Y  +LC+ +P+ + +S + C  +S MP ++F+IG + F LTP+ Y+ + GEG+   C S F A DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  360 GIQSVVDDGTSGL--LNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

[Close]

BLAST of GR399356 vs. TAIR peptide
Match: AT4G04460.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=508)

HSP 1 Score: 153.295 bits (386), Expect = 1.122e-37
Identity = 66/147 (44.90%), Postives = 100/147 (68.03%), Query Frame = 2

Query:    2 GIEMVTENKQSEMSATGTPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 442
            GI+ V ++  S +      +CS+C+M  +W++++L Q  T+ER+  Y  +LC+ +P+ + +S + C  +S MP ++F+IG + F LTP+ Y+ + GEG+   C S F A DI PP+GPLWILGD+FM  YHTVFDYG  +VGFA+AA
Sbjct:  364 GIQSVVDDGTSGL--LNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

[Close]

BLAST of GR399356 vs. TAIR peptide
Match: AT4G22050.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr4:11683865-11685506 FORWARD LENGTH=354)

HSP 1 Score: 63.929 bits (154), Expect = 8.950e-11
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 2

Query:  194 SCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGN---LQVGFAEA 439
            +CN+   +P+++FTIG K FVLTP  Y+ R+       C S F+           W LG  FMR +HTVFDY N   ++VGFA++
Sbjct:  276 NCNNFETLPDVTFTIGGKAFVLTPLDYIRRS----RSQCTSKFVG----KTNRSHWTLGIPFMRVFHTVFDYQNTLAVKVGFAKS 352

The following BLAST results are available for this feature:

BLAST of GR399356 vs. SwissProt
BLAST of GR399356 vs. TrEMBL
BLAST of GR399356 vs. TAIR peptide

BLAST of GR399356 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ASPRX_ORYSJ	6.254e-48	56.46	Aspartic proteinase OS=Oryza sativa subsp. japonic...	[more]
ASPR1_ORYSJ	8.168e-48	56.08	Aspartic proteinase oryzasin-1 OS=Oryza sativa sub...	[more]
ASPR_CUCPE	1.393e-47	58.11	Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1	[more]
CYPR1_CYNCA	1.180e-46	59.18	Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO...	[more]
ASPR_HORVU	3.432e-46	55.41	Phytepsin OS=Hordeum vulgare PE=1 SV=1	[more]
CARDB_CYNCA	9.671e-41	50.34	Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 ...	[more]
CARDA_CYNCA	9.671e-41	54.17	Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 ...	[more]
CATD_HUMAN	2.926e-21	50.00	Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1	[more]
CATD_BOVIN	3.821e-21	48.94	Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2	[more]
CATD_MOUSE	6.517e-21	47.31	Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1	[more]

BLAST of GR399356 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SXU0_CICAR	8.258e-80	99.32	Aspartic proteinase (Fragment) OS=Cicer arietinum ...	[more]
Q2HRQ7_MEDTR	7.999e-75	90.48	Peptidase A1, pepsin OS=Medicago truncatula GN=Mtr...	[more]
Q948P0_SOYBN	5.732e-73	88.36	Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=...	[more]
C6TDL8_SOYBN	4.853e-72	86.99	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TIU2_SOYBN	3.145e-71	88.11	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8H948_SOYBN	1.158e-65	78.23	Aspartic proteinase 5 (Fragment) OS=Glycine max GN...	[more]
B9I6C6_POPTR	2.338e-58	70.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q93XR0_IPOBA	1.160e-57	71.23	Putative aspartic protease OS=Ipomoea batatas PE=2...	[more]
B1Q478_CAPCH	4.127e-55	67.12	Putative aspartic protease OS=Capsicum chinense PE...	[more]
Q06EY4_IPOBA	1.201e-54	65.07	Aspartic protease OS=Ipomoea batatas PE=2 SV=1	[more]

BLAST of GR399356 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G11910.1	1.277e-49	59.46	\| Symbols: APA1, ATAPA1 \| aspartic proteinase A1 \|...	[more]
AT1G62290.2	2.409e-48	58.11	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT1G62290.1	2.409e-48	58.11	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G04460.2	1.122e-37	44.90	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G04460.1	1.122e-37	44.90	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G22050.1	8.950e-11	41.18	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Peptidase A1	PRINTS	PR00792	PEPSIN	coord: 12..23 score: 2.9e-05coord: 120..135 score: 2.9
IPR001461	Peptidase A1	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 39..146 score: 2.8
IPR001461	Peptidase A1	PFAM	PF00026	Asp	coord: 5..146 score: 6.8
IPR007856	Saposin-like type B, 1	PFAM	PF05184	SapB_1	coord: 19..56 score: 1.1
IPR008139	Saposin B	PROFILE	PS50015	SAP_B	coord: 17..58 score: 12
IPR009007	Peptidase aspartic, catalytic	GENE3D	2.40.70.10	no description	coord: 51..147 score: 2
IPR011001	Saposin-like	GENE3D	1.10.225.10	no description	coord: 19..50 score: 1.1
IPR011001	Saposin-like	SUPERFAMILY	47862	Saposin	coord: 2..56 score: 2.4
IPR021109	Peptidase aspartic	SUPERFAMILY	50630	Acid proteases	coord: 42..147 score: 2.5
None	No IPR available	PANTHER	PTHR13683:SF83	ASPARTIC PROTEINASE-RELATED	coord: 39..146 score: 2.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR399356 ID=GR399356; Name=GR399356; organism=Cicer arietinum; type=EST; length=677bp
TGGGATTGAGATGGTAACTGAAAATAAACAGAGTGAGATGTCAGCTACAG
GTACTCCTTTGTGTTCTTCTTGTCAGATGCTTGTTATTTGGGTCCAGAAT
CAACTAAAACAAAAGGCGACCAAGGAAAGAGTATTCAATTATGTGAATCA
ACTGTGTGAGAGTCTTCCAAGTCCATCCGGAGAATCAGTGATAAGCTGTA
ACGATCTTTCTCGGATGCCAAACATATCATTCACTATCGGAGACAAACCC
TTCGTCCTCACACCAGAACAGTATGTTCTAAGAACTGGAGAAGGCATTAC
AGAGGTCTGCCTTAGTGCGTTTATTGCTTTTGATATTCCTCCTCCAAAGG
GTCCACTCTGGATTCTTGGTGATGTTTTCATGAGGGCATATCACACAGTC
TTCGACTACGGAAACCTTCAAGTTGGTTTTGCAGAAGCTGCCTAGTTACC
ACATACCACTTATGGAGTAATATCTTACGGAATATTTTTCATAATTTCGT
TTATGCATTGTAAATAAGCTGTTGTGCTACTATGTAACTACTATACCTTT
AGGTTCACTTCATGCACCCCCATATATATGTTTTCCTGTACGCATTTATC
TCCTCTATCCCCCCTTTGATTCCCCCACCGCCTAATTTTTCACTACTTTA
AAGTTTGTGCCCCTTTTTCCTCAACAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis
GO:0006629	lipid metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR007856	SapB_1
IPR008139	SaposinB_dom
IPR009007	Peptidase aspartic, catalytic
IPR011001	Saposin-like
IPR021109	Peptidase_aspartic_dom_sf

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