GR403130

EST Overview


Name	GR403130
Unique Name	GR403130
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	341

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR403130 vs. SwissProt
Match: PDCD2_BOVIN (Programmed cell death protein 2 OS=Bos taurus GN=PDCD2 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.377e-11
Identity = 29/76 (38.16%), Postives = 39/76 (51.32%), Query Frame = 3

Query:   18 PIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQ 245
            P+W      P + DIP C  CG     EFQ++PQLL Y   D    S+DW  + ++ C  SC+  + Y  EF W Q
Sbjct:  264 PLWISGENTPKEKDIPDCP-CGAKRLFEFQVMPQLLNYLKADRLGRSVDWGVLAIFTCAESCKLGIGYTEEFVWKQ 338

[Close]

BLAST of GR403130 vs. SwissProt
Match: PDCD2_HUMAN (Programmed cell death protein 2 OS=Homo sapiens GN=PDCD2 PE=1 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 4.411e-11
Identity = 31/76 (40.79%), Postives = 37/76 (48.68%), Query Frame = 3

Query:   18 PIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQ 245
            PIW      P + DIP C  CG     EFQ++PQLL Y   D    S+DW  + V+ C  SC     Y  EF W Q
Sbjct:  264 PIWISGENIPQEKDIPDCP-CGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQ 338

[Close]

BLAST of GR403130 vs. SwissProt
Match: PDCD2_MOUSE (Programmed cell death protein 2 OS=Mus musculus GN=Pdcd2 PE=2 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 7.524e-11
Identity = 31/77 (40.26%), Postives = 38/77 (49.35%), Query Frame = 3

Query:   15 KPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQ 245
            KPIW      P + DIP C  CG     EFQ++PQLL +   D    S+DW  + V+ C  SC     Y  EF W Q
Sbjct:  262 KPIWISGENIPQEKDIPDCP-CGAKRIFEFQVMPQLLNHLKADRLGRSIDWGVLAVFTCAESCSLGSGYTEEFVWKQ 337

[Close]

BLAST of GR403130 vs. SwissProt
Match: PDCD2_RAT (Programmed cell death protein 2 (Fragment) OS=Rattus norvegicus GN=Pdcd2 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.676e-10
Identity = 30/77 (38.96%), Postives = 39/77 (50.65%), Query Frame = 3

Query:   15 KPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQ 245
            KPIW      P + DIP C  CG     EFQ++PQLL +   D    S+DW  + V+ C  SC   + +  EF W Q
Sbjct:  206 KPIWISGENIPQEKDIPDCS-CGVKRIFEFQVMPQLLNHLKADRLGTSVDWGILAVFTCAESCSLGIGFTEEFVWKQ 281

[Close]

BLAST of GR403130 vs. SwissProt
Match: YA99_SCHPO (Uncharacterized protein C13G6.09 OS=Schizosaccharomyces pombe GN=SPAC13G6.09 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.959e-7
Identity = 27/67 (40.30%), Postives = 38/67 (56.72%), Query Frame = 3

Query:   60 IPKCIYCGGLMCCEFQILPQLLYYFGVDNE-ENSLDWASIVVYACEASCE-ASLAYKNEFAWVQLYS 254
            IP C  CG     EFQILP L+    +D+  +N+LDW  + +Y C ASC+ A+     E  W+Q +S
Sbjct:  201 IPNCA-CGAKRQLEFQILPTLISSMNIDHSAKNALDWGILSIYVCSASCDLANCGLAEELCWLQTFS 266

[Close]

BLAST of GR403130 vs. TrEMBL
Match: C6TKD7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.485e-36
Identity = 71/86 (82.56%), Postives = 76/86 (88.37%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYSPS 260
            N++AKPIWP+ SGRPS  DIPKC YC G M CEFQILPQLLYYFGVDNE +SLDWASIVVYACEASCEASL YKNEFAWVQ+YS S
Sbjct:  318 NTNAKPIWPVSSGRPSNADIPKCSYCSGPMSCEFQILPQLLYYFGVDNEVDSLDWASIVVYACEASCEASLPYKNEFAWVQIYSLS 403

[Close]

BLAST of GR403130 vs. TrEMBL
Match: B9GSD8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816702 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.453e-31
Identity = 62/87 (71.26%), Postives = 72/87 (82.76%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYSPSA 263
            N+SAKP+WP+ SG+PSK DIP C YCGG    EFQILPQLLYYFGV N+ +SLDWA+IVVY C++SCEAS+AYK EF WVQLY  SA
Sbjct:  313 NASAKPLWPLSSGQPSKADIPNCSYCGGPSDFEFQILPQLLYYFGVKNDADSLDWATIVVYTCKSSCEASMAYKEEFPWVQLYPTSA 399

[Close]

BLAST of GR403130 vs. TrEMBL
Match: B9T078_RICCO (Pcdc2/rp-8, putative OS=Ricinus communis GN=RCOM_0769340 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 7.138e-31
Identity = 61/90 (67.78%), Postives = 73/90 (81.11%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYSPSAAKS 272
            N+SAKPIWP+ SG+PSK DIP C +CGG +  EFQ+LPQLLYYFGV N+ +SLDWA+I VY C  SCE+S++YK EFAWVQLYS SA  S
Sbjct:  296 NASAKPIWPMSSGQPSKADIPNCYHCGGPLIFEFQVLPQLLYYFGVKNDADSLDWATIAVYTCGESCESSVSYKEEFAWVQLYSQSATCS 385

[Close]

BLAST of GR403130 vs. TrEMBL
Match: Q93VB4_ARATH (Putative zinc finger protein OS=Arabidopsis thaliana GN=At4g02220 PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.260e-27
Identity = 54/84 (64.29%), Postives = 66/84 (78.57%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYS 254
            +S AKP+WPI SGR SK ++P C  CGG  C EFQ++PQLL++FG  NE  SLDWA+IVVY CE SC++SL+YK EF WVQLYS
Sbjct:  332 SSGAKPLWPIASGRVSKSELPSCKSCGGPRCFEFQVMPQLLFFFGGKNERESLDWATIVVYTCENSCDSSLSYKEEFVWVQLYS 415

[Close]

BLAST of GR403130 vs. TrEMBL
Match: O04261_ARATH (Putative zinc finger protein OS=Arabidopsis thaliana GN=T10M13.22 PE=4 SV=2)

HSP 1 Score: 125.946 bits (315), Expect = 1.260e-27
Identity = 54/84 (64.29%), Postives = 66/84 (78.57%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYS 254
            +S AKP+WPI SGR SK ++P C  CGG  C EFQ++PQLL++FG  NE  SLDWA+IVVY CE SC++SL+YK EF WVQLYS
Sbjct:  360 SSGAKPLWPIASGRVSKSELPSCKSCGGPRCFEFQVMPQLLFFFGGKNERESLDWATIVVYTCENSCDSSLSYKEEFVWVQLYS 443

[Close]

BLAST of GR403130 vs. TrEMBL
Match: D7U2D5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028059001 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.260e-27
Identity = 55/79 (69.62%), Postives = 65/79 (82.28%), Query Frame = 3

Query:   12 AKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQL 248
            AKP+WPI SGRPS+ D PKC YCGG    EFQILPQLLYYFGV ++ + LDW++I VY CEASCEASL+YK EFAW+Q+
Sbjct:  353 AKPVWPISSGRPSQVDAPKCRYCGGPSVFEFQILPQLLYYFGVKDDVDCLDWSTIAVYTCEASCEASLSYKEEFAWIQV 431

[Close]

BLAST of GR403130 vs. TrEMBL
Match: Q10MP9_ORYSJ (Os03g0300200 protein OS=Oryza sativa subsp. japonica GN=Os03g0300200 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.149e-27
Identity = 53/88 (60.23%), Postives = 68/88 (77.27%), Query Frame = 3

Query:    9 SAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYSPSAAKS 272
            +AKP+W + +G PS  DIP C YC G +C EFQI+PQLLYYFGV NE +SLDWA+IVVY C+ SC+ +++YK EFAWVQLY  +  +S
Sbjct:  332 NAKPLWALSAGCPSNADIPSCSYCRGPLCYEFQIMPQLLYYFGVKNEPDSLDWATIVVYTCKGSCDQNVSYKEEFAWVQLYPTTTTRS 419

[Close]

BLAST of GR403130 vs. TrEMBL
Match: B8AM25_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_11185 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.149e-27
Identity = 53/88 (60.23%), Postives = 68/88 (77.27%), Query Frame = 3

Query:    9 SAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYSPSAAKS 272
            +AKP+W + +G PS  DIP C YC G +C EFQI+PQLLYYFGV NE +SLDWA+IVVY C+ SC+ +++YK EFAWVQLY  +  +S
Sbjct:  332 NAKPLWALSAGCPSNADIPSCSYCRGPLCYEFQIMPQLLYYFGVKNEPDSLDWATIVVYTCKGSCDQNVSYKEEFAWVQLYPTTTTRS 419

[Close]

BLAST of GR403130 vs. TrEMBL
Match: D7M433_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_663711 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.807e-27
Identity = 53/84 (63.10%), Postives = 66/84 (78.57%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYS 254
            +S AKP+WPI SGR SK ++P C  CGG  C EFQ++PQLL++FG  N+  SLDWA+IVVY CE SC++SL+YK EF WVQLYS
Sbjct:  333 SSGAKPLWPIASGRVSKSELPNCKSCGGPRCFEFQVMPQLLFFFGGKNDRESLDWATIVVYTCENSCDSSLSYKEEFVWVQLYS 416

[Close]

BLAST of GR403130 vs. TrEMBL
Match: C5WNE5_SORBI (Putative uncharacterized protein Sb01g037990 OS=Sorghum bicolor GN=Sb01g037990 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 4.053e-26
Identity = 50/81 (61.73%), Postives = 64/81 (79.01%), Query Frame = 3

Query:    9 SAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLY 251
            +AKP+W + SG PS  DIP C YC G +C EFQ++PQLLY+FGV N+ +SLDWA+I VY C+ SC+ S++YK EFAWVQLY
Sbjct:  329 NAKPLWALSSGCPSNADIPSCSYCRGPLCYEFQLMPQLLYFFGVGNQPDSLDWATIAVYTCQGSCDQSVSYKEEFAWVQLY 409

[Close]

BLAST of GR403130 vs. TAIR peptide
Match: AT4G02220.1 (| Symbols: | zinc finger (MYND type) family protein / programmed cell death 2 C-terminal domain-containing protein | chr4:976728-978902 FORWARD LENGTH=418)

HSP 1 Score: 125.946 bits (315), Expect = 4.868e-30
Identity = 54/84 (64.29%), Postives = 66/84 (78.57%), Query Frame = 3

Query:    3 NSSAKPIWPILSGRPSKDDIPKCIYCGGLMCCEFQILPQLLYYFGVDNEENSLDWASIVVYACEASCEASLAYKNEFAWVQLYS 254
            +S AKP+WPI SGR SK ++P C  CGG  C EFQ++PQLL++FG  NE  SLDWA+IVVY CE SC++SL+YK EF WVQLYS
Sbjct:  332 SSGAKPLWPIASGRVSKSELPSCKSCGGPRCFEFQVMPQLLFFFGGKNERESLDWATIVVYTCENSCDSSLSYKEEFVWVQLYS 415

The following BLAST results are available for this feature:

BLAST of GR403130 vs. SwissProt
BLAST of GR403130 vs. TrEMBL
BLAST of GR403130 vs. TAIR peptide

BLAST of GR403130 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
PDCD2_BOVIN	3.377e-11	38.16	Programmed cell death protein 2 OS=Bos taurus GN=P...	[more]
PDCD2_HUMAN	4.411e-11	40.79	Programmed cell death protein 2 OS=Homo sapiens GN...	[more]
PDCD2_MOUSE	7.524e-11	40.26	Programmed cell death protein 2 OS=Mus musculus GN...	[more]
PDCD2_RAT	1.676e-10	38.96	Programmed cell death protein 2 (Fragment) OS=Ratt...	[more]
YA99_SCHPO	2.959e-7	40.30	Uncharacterized protein C13G6.09 OS=Schizosaccharo...	[more]

BLAST of GR403130 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TKD7_SOYBN	1.485e-36	82.56	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9GSD8_POPTR	2.453e-31	71.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T078_RICCO	7.138e-31	67.78	Pcdc2/rp-8, putative OS=Ricinus communis GN=RCOM_0...	[more]
Q93VB4_ARATH	1.260e-27	64.29	Putative zinc finger protein OS=Arabidopsis thalia...	[more]
O04261_ARATH	1.260e-27	64.29	Putative zinc finger protein OS=Arabidopsis thalia...	[more]
D7U2D5_VITVI	1.260e-27	69.62	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q10MP9_ORYSJ	2.149e-27	60.23	Os03g0300200 protein OS=Oryza sativa subsp. japoni...	[more]
B8AM25_ORYSI	2.149e-27	60.23	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7M433_ARALY	2.807e-27	63.10	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
C5WNE5_SORBI	4.053e-26	61.73	Putative uncharacterized protein Sb01g037990 OS=So...	[more]

BLAST of GR403130 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G02220.1	4.868e-30	64.29	\| Symbols: \| zinc finger (MYND type) family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007320	Programmed cell death protein 2, C-terminal	PFAM	PF04194	PDCD2_C	coord: 3..82 score: 5.1
None	No IPR available	PANTHER	PTHR12298	PCDC2 (PROGRAMMED CELL DEATH PROTEIN 2)-RELATED	coord: 4..86 score: 2.8
None	No IPR available	PANTHER	PTHR12298:SF4	PCDC2/RP-8 (PROGRAMMED CELL DEATH PROTEIN 2)	coord: 4..86 score: 2.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR403130 ID=GR403130; Name=GR403130; organism=Cicer arietinum; type=EST; length=341bp
GGAACTCTAGTGCTAAGCCAATATGGCCCATTTTAAGTGGTCGGCCATCC
AAAGATGATATCCCTAAATGCATTTATTGTGGTGGACTCATGTGTTGTGA
ATTTCAGATTTTGCCACAGCTGTTGTATTATTTTGGGGTGGACAATGAAG
AGAACTCTTTGGATTGGGCTTCGATCGTTGTGTATGCATGTGAAGCATCT
TGTGAAGCAAGTTTGGCTTACAAGAATGAATTTGCTTGGGTTCAACTTTA
TTCACCTTCTGCTGCCAAATCGTGAGAAATTTTAGTTCTAAGTTAAGTTT
GCATCTGTTAGAAATTTTTTTGCCCCCTTTGCAGATATAAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR007320	PDCD2_C

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