GR408416

EST Overview


Name	GR408416
Unique Name	GR408416
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	653

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408416 vs. TrEMBL
Match: D7MC47_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_491075 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.055e-20
Identity = 59/86 (68.60%), Postives = 67/86 (77.91%), Query Frame = 2

Query:   35 KRIFKLTLSLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRVILHT*SQMQIHFYN--YDIFC 286
            K++ K +LSL   GFNSSKCKTAAKMAVARIKLLRNKR VVV+QMRRDIA+LLQSGQDATARIRV  H   +  I   N   ++FC
Sbjct:   12 KKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLLRNKRLVVVKQMRRDIAVLLQSGQDATARIRV-EHVIREQNIQAANEIIELFC 96

[Close]

BLAST of GR408416 vs. TrEMBL
Match: B9GZ83_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_554016 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.799e-20
Identity = 54/65 (83.08%), Postives = 58/65 (89.23%), Query Frame = 2

Query:   35 KRIFKLTLSLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            K+  KLT+S+    FNSSKCKTAAKMAVARIKLLRNKRE V+RQMRRDIALLLQSGQDATARIRV
Sbjct:   12 KKFMKLTISIFCPRFNSSKCKTAAKMAVARIKLLRNKREAVMRQMRRDIALLLQSGQDATARIRV 76

[Close]

BLAST of GR408416 vs. TrEMBL
Match: B9S6Q8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0871920 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 6.835e-20
Identity = 51/65 (78.46%), Postives = 59/65 (90.77%), Query Frame = 2

Query:   35 KRIFKLTLSLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            K++ K TLS+    F+SS+CKTAAKMAVARIKLLRNKREVVVRQM+RDIALLL+SGQDATAR+RV
Sbjct:   12 KKLMKFTLSIFSPRFSSSRCKTAAKMAVARIKLLRNKREVVVRQMKRDIALLLESGQDATARVRV 76

[Close]

BLAST of GR408416 vs. TrEMBL
Match: D7TS00_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_1235.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00004308001 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.431e-19
Identity = 52/61 (85.25%), Postives = 55/61 (90.16%), Query Frame = 2

Query:   47 KLTLSLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            KL LS+   GF+SSKCKT AKMA+ARIKLLRNKRE VVRQMRRDIALLLQSGQDATARIRV
Sbjct:    2 KLGLSIFRRGFSSSKCKTMAKMAMARIKLLRNKREAVVRQMRRDIALLLQSGQDATARIRV 62

[Close]

BLAST of GR408416 vs. TrEMBL
Match: D7TXV0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_164.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001596001 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.803e-12
Identity = 36/57 (63.16%), Postives = 45/57 (78.95%), Query Frame = 2

Query:   59 SLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            S    GF  SKCKT  K+ + RIKLLRN+RE+ ++QMRRDIA LL++GQ+ATARIRV
Sbjct:    6 SFFNKGFKGSKCKTLLKLTIPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARIRV 62

[Close]

BLAST of GR408416 vs. TrEMBL
Match: A5B547_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034688 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.803e-12
Identity = 36/57 (63.16%), Postives = 45/57 (78.95%), Query Frame = 2

Query:   59 SLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            S    GF  SKCKT  K+ + RIKLLRN+RE+ ++QMRRDIA LL++GQ+ATARIRV
Sbjct:    6 SFFNKGFKGSKCKTLLKLTIPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARIRV 62

[Close]

BLAST of GR408416 vs. TrEMBL
Match: B9T8C2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0186330 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.075e-12
Identity = 35/57 (61.40%), Postives = 46/57 (80.70%), Query Frame = 2

Query:   59 SLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            S    GF ++KCKT  K+ + RIKLLRN+RE+ ++QMRRDIA LL++GQ+ATARIRV
Sbjct:    6 SFFNKGFKATKCKTLLKLTIPRIKLLRNRREIQIKQMRRDIAKLLETGQEATARIRV 62

[Close]

BLAST of GR408416 vs. TrEMBL
Match: Q6Z4T7_ORYSJ (Os08g0175200 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0054L03.38 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.017e-12
Identity = 36/54 (66.67%), Postives = 46/54 (85.19%), Query Frame = 2

Query:   68 GLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            G GF  +KCKT  K+++ RIKLLRN+RE+ +RQMRRDIA LL++GQ+ATARIRV
Sbjct:   13 GGGFRGAKCKTLLKLSIPRIKLLRNRRELQLRQMRRDIAKLLEAGQEATARIRV 66

[Close]

BLAST of GR408416 vs. TrEMBL
Match: C0PHV9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.017e-12
Identity = 36/54 (66.67%), Postives = 46/54 (85.19%), Query Frame = 2

Query:   68 GLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            G GF  +KCKT  K+++ RIKLLRN+RE+ +RQMRRDIA LL++GQ+ATARIRV
Sbjct:   13 GGGFRGAKCKTLLKLSIPRIKLLRNRRELQLRQMRRDIAKLLEAGQEATARIRV 66

[Close]

BLAST of GR408416 vs. TrEMBL
Match: B4FSB7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.017e-12
Identity = 36/54 (66.67%), Postives = 46/54 (85.19%), Query Frame = 2

Query:   68 GLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            G GF  +KCKT  K+++ RIKLLRN+RE+ +RQMRRDIA LL++GQ+ATARIRV
Sbjct:   11 GGGFRGAKCKTLLKLSIPRIKLLRNRRELQLRQMRRDIAKLLEAGQEATARIRV 64

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT4G35730.1 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr4:16931111-16933241 FORWARD LENGTH=466)

HSP 1 Score: 103.219 bits (256), Expect = 1.262e-22
Identity = 58/86 (67.44%), Postives = 67/86 (77.91%), Query Frame = 2

Query:   35 KRIFKLTLSLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRVILHT*SQMQIHFYN--YDIFC 286
            K++ K +LSL   GFNSSKCKTAAKMAVARIKL+RNKR VVV+QMRRDIA+LLQSGQDATARIRV  H   +  I   N   ++FC
Sbjct:   12 KKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRV-EHVIREQNIQAANEIIELFC 96

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT1G34220.2 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr1:12463102-12465911 REVERSE LENGTH=619)

HSP 1 Score: 72.4034 bits (176), Expect = 2.386e-13
Identity = 33/57 (57.89%), Postives = 45/57 (78.95%), Query Frame = 2

Query:   59 SLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            S    GF ++KCKT  K+ + RIKL+RN+RE  ++QMRR+IA LL++GQ+ATARIRV
Sbjct:    6 SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRV 62

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT1G34220.1 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr1:12463102-12465911 REVERSE LENGTH=649)

HSP 1 Score: 72.4034 bits (176), Expect = 2.386e-13
Identity = 33/57 (57.89%), Postives = 45/57 (78.95%), Query Frame = 2

Query:   59 SLLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            S    GF ++KCKT  K+ + RIKL+RN+RE  ++QMRR+IA LL++GQ+ATARIRV
Sbjct:    6 SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRV 62

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT2G19710.1 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr2:8506339-8509817 FORWARD LENGTH=937)

HSP 1 Score: 68.1662 bits (165), Expect = 4.500e-12
Identity = 31/56 (55.36%), Postives = 46/56 (82.14%), Query Frame = 2

Query:   62 LLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            +L  GF  +KCKTA +MA +R+K+L+NK+E+ ++Q+RR++A LL+SGQ  TARIRV
Sbjct:    4 VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRV 59

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT4G29440.1 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr4:14473942-14477721 REVERSE LENGTH=1090)

HSP 1 Score: 60.8474 bits (146), Expect = 7.185e-10
Identity = 27/56 (48.21%), Postives = 43/56 (76.79%), Query Frame = 2

Query:   62 LLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            +L   F  +KCK A +MA +R+K+L+NK++  ++Q+RR++A LL+SGQ  TA+IRV
Sbjct:    4 VLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRV 59

[Close]

BLAST of GR408416 vs. TAIR peptide
Match: AT1G25420.1 (| Symbols: | Regulator of Vps4 activity in the MVB pathway protein | chr1:8916141-8917980 FORWARD LENGTH=323)

HSP 1 Score: 59.3066 bits (142), Expect = 2.091e-9
Identity = 27/56 (48.21%), Postives = 42/56 (75.00%), Query Frame = 2

Query:   62 LLGLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQSGQDATARIRV 229
            L   G   +KCKT+  +A+AR+KLL+NKR++ ++ M+++IA  LQ+GQ+  ARIRV
Sbjct:    7 LFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRV 62

The following BLAST results are available for this feature:

BLAST of GR408416 vs. TrEMBL
BLAST of GR408416 vs. TAIR peptide

BLAST of GR408416 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7MC47_ARALY	1.055e-20	68.60	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9GZ83_POPTR	1.799e-20	83.08	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S6Q8_RICCO	6.835e-20	78.46	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7TS00_VITVI	4.431e-19	85.25	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TXV0_VITVI	1.803e-12	63.16	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B547_VITVI	1.803e-12	63.16	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9T8C2_RICCO	3.075e-12	61.40	Putative uncharacterized protein OS=Ricinus commun...	[more]
Q6Z4T7_ORYSJ	4.017e-12	66.67	Os08g0175200 protein OS=Oryza sativa subsp. japoni...	[more]
C0PHV9_MAIZE	4.017e-12	66.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FSB7_MAIZE	4.017e-12	66.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GR408416 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT4G35730.1	1.262e-22	67.44	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]
AT1G34220.2	2.386e-13	57.89	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]
AT1G34220.1	2.386e-13	57.89	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]
AT2G19710.1	4.500e-12	55.36	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]
AT4G29440.1	7.185e-10	48.21	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]
AT1G25420.1	2.091e-9	48.21	\| Symbols: \| Regulator of Vps4 activity in the MV...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005061	Domain of unknown function DUF292, eukaryotic	PFAM	PF03398	Ist1	coord: 32..76 score: 3
None	No IPR available	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 51..76 score: 3
None	No IPR available	PANTHER	PTHR12161:SF4	MJK13.15 PROTEIN	coord: 51..76 score: 3
None	No IPR available	SEG	seg	seg	coord: 2..11 score
None	No IPR available	SEG	seg	seg	coord: 14..30 score: NAcoord: 38..49 score: NAcoord: 57..66 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408416 ID=GR408416; Name=GR408416; organism=Cicer arietinum; type=EST; length=653bp
GATGACGGTCGCAGCCACAGCCACTGCACGCACCAAGAGAATCTTCAAGC
TCACTCTCTCCCTTCTCGGTCTCGGATTCAACTCTTCCAAATGTAAAACG
GCGGCGAAGATGGCGGTGGCCAGGATTAAGCTGTTAAGGAACAAGAGGGA
GGTGGTGGTTAGGCAGATGAGACGCGACATTGCGCTTCTTCTCCAGTCTG
GTCAAGATGCCACCGCTCGTATCAGGGTAATTTTACACACTTAATCACAA
ATGCAGATTCATTTTTATAATTATGATATTTTTTGTTACACCCGCGTGTG
AGATTAGAACAATCGACTTCCATATCACTCTACAATCTATTTTCAGTTTA
GTTACATAAGTCTAGGATATATTATGAAAAGTATAGTTTGATTTTTATTT
ATTTTTTGTTATAGAAATAAACTATACTGTAAGTGCTTTAATTAAGCGTT
TATCCAAACAAGACCTTGGTCCAACCATGTTCATCCATTGCAAACCAAGG
TTGAGGGGTTCCCATCATGAAACTGAACATGGTTGTGTTAATTGTATAGA
GCAGAGGTAAACATGCCTAGTGAATTTGTGGTTGGAATTTGATGACAGAG
TATCTGATTCAGTATCTATCTTTGTTTGATTAGCTATGTGTCATGTTGCT
TTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR005061	Ist1

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