GR420933

EST Overview


Name	GR420933
Unique Name	GR420933
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	606

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR420933 vs. SwissProt
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.542e-17
Identity = 59/141 (41.84%), Postives = 84/141 (59.57%), Query Frame = 2

Query:  128 RSTKPS----DMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGS----TDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            R++KPS    + +EV+ VF R  A     IS  EL  VL+ L S+ S      IME++DTDK     DGFI     AAF ++ +    + GG +EL++AF+LYD+D NGLIS+ EL ++L+    +   ++C  MIKSVDS
Sbjct:    7 RNSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDK-----DGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKSVDS 142

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BLAST of GR420933 vs. SwissProt
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.725e-16
Identity = 51/140 (36.43%), Postives = 80/140 (57.14%), Query Frame = 2

Query:   92 NTETSESNRHLLRSTKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            N ET++     +    P   +E+K+VF +  +     ISV EL  V + + +S ++   + ++E++DTD+    +    +  CRS S+   A+E+RDAFDLYD+DKNGLISA+EL QVL+         +C  MI  VD+
Sbjct:    5 NPETAKPTPATVDMANP---EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTLCRSSSS---AAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 138

[Close]

BLAST of GR420933 vs. SwissProt
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.539e-15
Identity = 54/145 (37.24%), Postives = 84/145 (57.93%), Query Frame = 2

Query:   92 NTETSESNRHLLRSTKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            NT    +N+    ST PS   E+K+VF +  A     ISV+EL NV +++ +S ++   + +++++D D      DGFI     A  CRS S+   A E+R+AFDLYD++KNGLIS++E+ +VL+         +C  MI  VD+
Sbjct:    3 NTNLESTNK----STTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID-----CDGFINQEEFATICRSSSS---AVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 135

[Close]

BLAST of GR420933 vs. SwissProt
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.852e-12
Identity = 45/124 (36.29%), Postives = 65/124 (52.42%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRS--GSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            M+++K+VF R        IS+ EL +V+  L  +AS      +M++ D D       D F+A F  S   S +    +L++AFDLYD D+NG ISA EL  V+     K    +C+ MI  VDS
Sbjct:   13 MEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKVDS 136

[Close]

BLAST of GR420933 vs. SwissProt
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 4.954e-11
Identity = 46/125 (36.80%), Postives = 62/125 (49.60%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFC----RSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            MD++K+VF R        ISV EL  V+R L  +AS      +M+  D D       D F+A F       G+     S+L++AF+LYD D NG ISA EL  V+     K    +C+ MI  VD
Sbjct:   15 MDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKVD 139

[Close]

BLAST of GR420933 vs. SwissProt
Match: CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica GN=CML16 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.451e-11
Identity = 52/128 (40.62%), Postives = 65/128 (50.78%), Query Frame = 2

Query:  140 PSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLR----SSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGG-ASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            P+D  E++RVFTR  A     IS +EL  V R +      SA  R    +M +LDTD+      G  AAF   G  D    +ELR AFD+YD D +G I+A EL +VL          EC  MI SVD
Sbjct:   23 PADA-EIERVFTRFDADGDGRISPSELAAVTRAIAPPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRGRGDAEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIGEGCSAEECERMIASVD 149

[Close]

BLAST of GR420933 vs. SwissProt
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.883e-10
Identity = 49/133 (36.84%), Postives = 67/133 (50.38%), Query Frame = 2

Query:  131 STKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASE--LRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            S  P+  +E++RVF +  A     IS +EL  +  +L  +A+D     +M + D D      DGFI     AA   + S D  A E  LR AF ++D D NG ISA EL +VL     K    +CR MI+ VD
Sbjct:   32 SPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADAD-----GDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

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BLAST of GR420933 vs. SwissProt
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.220e-9
Identity = 50/139 (35.97%), Postives = 69/139 (49.64%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAF-CRSGSTDGGA------------SELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            M E+++VF R  A     IS  EL +VLR L +         +M+++D+D+     DGF+     AAF C      GG             +ELR+AF +YD D NG ISA EL +VL     K    +C  MI+SVD+
Sbjct:    3 MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDR-----DGFVDLSEFAAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSVADCSRMIRSVDA 136

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BLAST of GR420933 vs. SwissProt
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 7.910e-9
Identity = 38/120 (31.67%), Postives = 61/120 (50.83%), Query Frame = 2

Query:  155 EVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAA--FCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            E++ VF +  A     IS +EL ++LR+L S   +     +ME+ D D      DG+++   F    +      +L++AF ++D+D NG ISA ELC  L          E +N+I +VD
Sbjct:   26 ELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADAD-----GDGYVSLQEFVDLNNKGASVKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNVD 140

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BLAST of GR420933 vs. SwissProt
Match: CML8_ARATH (Calmodulin-like protein 8 OS=Arabidopsis thaliana GN=CML8 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 6.696e-8
Identity = 36/119 (30.25%), Postives = 56/119 (47.06%), Query Frame = 2

Query:  155 EVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDG-FIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            E K  F          I+V EL  V+R+L  + +++  H I+ ++D+D         F+    +         EL++AF ++DKD+NG ISA+EL  V+     K    E   MIK  D
Sbjct:   13 EFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQMIKEAD 131

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BLAST of GR420933 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.143e-31
Identity = 88/165 (53.33%), Postives = 98/165 (59.39%), Query Frame = 2

Query:   83 TEQNTETSESNRHLLRSTKPS----DMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDSKXXXXXXXXWFKQ 550
            TEQN  T          TKPS    DMDE+KRVF+R  A     ISV ELDNVLR L S+        +M+DLDTD     +DGFI     AAFCRS + DGGASELR+AFDLYD+DKNGLISA ELC VL+    K    EC NMIKSVDS          FKQ
Sbjct:   13 TEQNPATK---------TKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGSTVPSDELERVMKDLDTD-----NDGFINLTEFAAFCRSDAADGGASELREAFDLYDQDKNGLISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQ 163

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BLAST of GR420933 vs. TrEMBL
Match: C6T2C2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 4.333e-28
Identity = 77/136 (56.62%), Postives = 88/136 (64.71%), Query Frame = 2

Query:  131 STKPS----DMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            +TKPS    D +E+KRVF+R  A     ISVTELDNVLR+L S         +M+DLDTD      DGFI     AAFCRS + DGG +EL DAF+LYD DKNG ISATELCQVL+    K    EC NMIKSVDS
Sbjct:   20 TTKPSVYLQDTEELKRVFSRFDANCDGKISVTELDNVLRSLGSGVPPEDIQRVMDDLDTDH-----DGFINLSEFAAFCRSDTADGGDAELHDAFNLYDHDKNGHISATELCQVLNRLGMKCSVEECHNMIKSVDS 150

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BLAST of GR420933 vs. TrEMBL
Match: D3Y1S3_9FABA (Calmodulin 24-like protein (Fragment) OS=Eperua grandiflora GN=Cml24 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.303e-21
Identity = 63/108 (58.33%), Postives = 72/108 (66.67%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            I + ELDNVLRTL SSA  +    +M+DLDTD      DGFI     A FCRS + DGG SEL DAF+LYD+DKNGLIS TEL  VL+        +ECRNMIKSVDS
Sbjct:    1 ICINELDNVLRTLGSSAPPQELRRVMDDLDTDH-----DGFINLLEFADFCRSDTADGGDSELHDAFNLYDQDKNGLISDTELHMVLNRLGLNCSVDECRNMIKSVDS 103

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BLAST of GR420933 vs. TrEMBL
Match: D3Y1S4_9FABA (Calmodulin 24-like protein (Fragment) OS=Eperua grandiflora GN=Cml24 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 6.701e-21
Identity = 62/108 (57.41%), Postives = 71/108 (65.74%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            I + ELDNVLRTL SS   +    +M+DLDTD      DGFI     A FCRS + DGG SEL DAF+LYD+DKNGLIS TEL  VL+        +ECRNMIKSVDS
Sbjct:    1 ICINELDNVLRTLGSSVPPQELRRVMDDLDTDH-----DGFINLLEFADFCRSDTADGGDSELHDAFNLYDQDKNGLISDTELHMVLNRLGLNCSVDECRNMIKSVDS 103

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BLAST of GR420933 vs. TrEMBL
Match: D3Y1R9_9FABA (Calmodulin 24-like protein (Fragment) OS=Eperua falcata GN=Cml24 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 8.752e-21
Identity = 62/108 (57.41%), Postives = 72/108 (66.67%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            I + EL NVLRTL SS S +    +M+DLDTD      DGFI     A FCRS + DGG SEL+DAF+LYD+DKNGLIS TEL  VL+        +ECRNMIKSVDS
Sbjct:    1 ICINELGNVLRTLGSSVSPQELRRVMDDLDTDH-----DGFINLLEFADFCRSDTADGGDSELQDAFNLYDQDKNGLISDTELHMVLNRLGLNCSVDECRNMIKSVDS 103

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BLAST of GR420933 vs. TrEMBL
Match: D3Y1T6_9FABA (Calmodulin 24-like protein (Fragment) OS=Tachigali melinonii GN=Cml24 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.673e-20
Identity = 58/103 (56.31%), Postives = 65/103 (63.11%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            ISVTELDNVLRTL S         +M+DLD D     +    A FCRS + DGGASELRDAF+LYD DKNGLISATEL   L+    K    EC  MI+ VDS
Sbjct:    1 ISVTELDNVLRTLGSGVPPEGLQSVMDDLDLDHDGYINFSEFAEFCRSDTADGGASELRDAFNLYDHDKNGLISATELHMALNRLGLKCTVEECHVMIQPVDS 103

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BLAST of GR420933 vs. TrEMBL
Match: A5ATQ5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_014971 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.815e-19
Identity = 64/151 (42.38%), Postives = 83/151 (54.97%), Query Frame = 2

Query:  110 SNRHLLRSTKPS----DMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDSKXXXXXXXXWFKQ 550
            ++  +  STKP+    D DE+++VF R  A     IS +EL NVLR L S +S      +M+++DTD     +    A FC+SGS +  A ELRDAF LYD DKNGLISA EL QVL     K    +C+ MI S DS          FK+
Sbjct:    2 TSNSISESTKPNIYPQDKDELQKVFNRFDANGDGKISSSELANVLRALGSESSPEEMSRVMKEIDTDDDGCINLEEFAQFCKSGS-NADAGELRDAFQLYDGDKNGLISAVELHQVLKQLGEKCSVQDCQKMIGSFDSDGDGNISFDEFKE 151

[Close]

BLAST of GR420933 vs. TrEMBL
Match: D3Y1T1_9FABA (Calmodulin 24-like protein (Fragment) OS=Bauhinia guianensis GN=Cml24 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.677e-19
Identity = 59/108 (54.63%), Postives = 69/108 (63.89%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            IS  EL NVLRTL S+        +M++LDTD      DGFI     AAFCR+G+ DGG+SEL +AF LYD+DKNGLISA EL  VL+    K    EC  MIKSVDS
Sbjct:    1 ISFDELGNVLRTLGSAVPRDELQQVMDELDTDH-----DGFINLSEFAAFCRAGTEDGGSSELHEAFQLYDQDKNGLISANELHLVLNRLGMKCSMEECHRMIKSVDS 103

[Close]

BLAST of GR420933 vs. TrEMBL
Match: D3Y1S9_BAUPU (Calmodulin 24-like protein (Fragment) OS=Bauhinia purpurea GN=Cml24 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.677e-19
Identity = 56/103 (54.37%), Postives = 67/103 (65.05%), Query Frame = 2

Query:  203 ISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            IS+TELD+VLRTL S         +M++LDTD     +    AAFCR+ + DGGASEL DAF  YD+DKNGLISATEL  VL+    K    EC  MI+SVDS
Sbjct:    1 ISITELDDVLRTLGSGVPPADLKNVMDELDTDHDGVINLQEFAAFCRANTEDGGASELHDAFQHYDQDKNGLISATELHMVLNRLGMKCSVEECHRMIESVDS 103

[Close]

BLAST of GR420933 vs. TrEMBL
Match: B9IEB3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_666293 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.310e-18
Identity = 54/122 (44.26%), Postives = 73/122 (59.84%), Query Frame = 2

Query:  146 DMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            D  E+++VF +        IS +EL  VL+++ S+ +    H +MED+DTDK         A  CRS S    ASELRDAFDLYD++ +G+ISA EL QVL+    K   +EC  MIK+VDS
Sbjct:   25 DTAELRKVFNQFDTNGDGKISASELGEVLKSMGSTYTMEELHRVMEDVDTDKDGYIDLAEFAKLCRSSSAAAAASELRDAFDLYDQNGDGMISAAELHQVLNRLGMKCKVDECFQMIKNVDS 146

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT1G18210.2 (| Symbols: | Calcium-binding EF-hand family protein | chr1:6268273-6268785 REVERSE LENGTH=170)

HSP 1 Score: 83.5741 bits (205), Expect = 9.097e-17
Identity = 51/140 (36.43%), Postives = 80/140 (57.14%), Query Frame = 2

Query:   92 NTETSESNRHLLRSTKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            N ET++     +    P   +E+K+VF +  +     ISV EL  V + + +S ++   + ++E++DTD+    +    +  CRS S+   A+E+RDAFDLYD+DKNGLISA+EL QVL+         +C  MI  VD+
Sbjct:    5 NPETAKPTPATVDMANP---EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTLCRSSSS---AAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 138

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT1G18210.1 (| Symbols: | Calcium-binding EF-hand family protein | chr1:6268273-6268785 REVERSE LENGTH=170)

HSP 1 Score: 83.5741 bits (205), Expect = 9.097e-17
Identity = 51/140 (36.43%), Postives = 80/140 (57.14%), Query Frame = 2

Query:   92 NTETSESNRHLLRSTKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            N ET++     +    P   +E+K+VF +  +     ISV EL  V + + +S ++   + ++E++DTD+    +    +  CRS S+   A+E+RDAFDLYD+DKNGLISA+EL QVL+         +C  MI  VD+
Sbjct:    5 NPETAKPTPATVDMANP---EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTLCRSSSS---AAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 138

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT1G73630.1 (| Symbols: | EF hand calcium-binding protein family | chr1:27684748-27685239 FORWARD LENGTH=163)

HSP 1 Score: 82.0333 bits (201), Expect = 2.647e-16
Identity = 54/145 (37.24%), Postives = 84/145 (57.93%), Query Frame = 2

Query:   92 NTETSESNRHLLRSTKPSDMDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDGFI-----AAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            NT    +N+    ST PS   E+K+VF +  A     ISV+EL NV +++ +S ++   + +++++D D      DGFI     A  CRS S+   A E+R+AFDLYD++KNGLIS++E+ +VL+         +C  MI  VD+
Sbjct:    3 NTNLESTNK----STTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID-----CDGFINQEEFATICRSSSS---AVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 135

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT1G66400.1 (| Symbols: CML23 | calmodulin like 23 | chr1:24770856-24771329 REVERSE LENGTH=157)

HSP 1 Score: 70.8626 bits (172), Expect = 6.102e-13
Identity = 45/124 (36.29%), Postives = 65/124 (52.42%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRS--GSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVDS 511
            M+++K+VF R        IS+ EL +V+  L  +AS      +M++ D D       D F+A F  S   S +    +L++AFDLYD D+NG ISA EL  V+     K    +C+ MI  VDS
Sbjct:   13 MEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKVDS 136

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT5G37770.1 (| Symbols: TCH2, CML24 | EF hand calcium-binding protein family | chr5:14999075-14999560 REVERSE LENGTH=161)

HSP 1 Score: 67.781 bits (164), Expect = 5.165e-12
Identity = 46/125 (36.80%), Postives = 62/125 (49.60%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFC----RSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            MD++K+VF R        ISV EL  V+R L  +AS      +M+  D D       D F+A F       G+     S+L++AF+LYD D NG ISA EL  V+     K    +C+ MI  VD
Sbjct:   15 MDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKVD 139

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT4G14640.1 (| Symbols: CAM8, AtCML8 | calmodulin 8 | chr4:8397800-8399996 FORWARD LENGTH=151)

HSP 1 Score: 57.3806 bits (137), Expect = 6.981e-9
Identity = 36/119 (30.25%), Postives = 56/119 (47.06%), Query Frame = 2

Query:  155 EVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSSDG-FIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            E K  F          I+V EL  V+R+L  + +++  H I+ ++D+D         F+    +         EL++AF ++DKD+NG ISA+EL  V+     K    E   MIK  D
Sbjct:   13 EFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQMIKEAD 131

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT3G56800.1 (| Symbols: CAM3, acam-3 | calmodulin 3 | chr3:21034981-21035920 REVERSE LENGTH=149)

HSP 1 Score: 51.6026 bits (122), Expect = 3.831e-7
Identity = 34/121 (28.10%), Postives = 54/121 (44.63%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            + E K  F+         I+  EL  V+R+L  + ++     ++ ++D D         F+    R         EL++AF ++DKD+NG ISA EL  V++    K    E   MIK  D
Sbjct:   10 ISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEAD 130

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT2G27030.3 (| Symbols: CAM5 | calmodulin 5 | chr2:11532069-11534176 FORWARD LENGTH=181)

HSP 1 Score: 51.6026 bits (122), Expect = 3.831e-7
Identity = 34/121 (28.10%), Postives = 54/121 (44.63%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            + E K  F+         I+  EL  V+R+L  + ++     ++ ++D D         F+    R         EL++AF ++DKD+NG ISA EL  V++    K    E   MIK  D
Sbjct:   10 ISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEAD 130

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT2G27030.1 (| Symbols: CAM5, ACAM-2 | calmodulin 5 | chr2:11532069-11533060 FORWARD LENGTH=149)

HSP 1 Score: 51.6026 bits (122), Expect = 3.831e-7
Identity = 34/121 (28.10%), Postives = 54/121 (44.63%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            + E K  F+         I+  EL  V+R+L  + ++     ++ ++D D         F+    R         EL++AF ++DKD+NG ISA EL  V++    K    E   MIK  D
Sbjct:   10 ISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEAD 130

[Close]

BLAST of GR420933 vs. TAIR peptide
Match: AT2G41110.1 (| Symbols: CAM2, ATCAL5 | calmodulin 2 | chr2:17140379-17141192 FORWARD LENGTH=149)

HSP 1 Score: 51.6026 bits (122), Expect = 3.831e-7
Identity = 34/121 (28.10%), Postives = 54/121 (44.63%), Query Frame = 2

Query:  149 MDEVKRVFTRLTATTPTAISVTELDNVLRTLRSSASDRVFHMIMEDLDTDKTSRSS-DGFIAAFCRSGSTDGGASELRDAFDLYDKDKNGLISATELCQVLSVPLRKQPRNECRNMIKSVD 508
            + E K  F+         I+  EL  V+R+L  + ++     ++ ++D D         F+    R         EL++AF ++DKD+NG ISA EL  V++    K    E   MIK  D
Sbjct:   10 ISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEAD 130

The following BLAST results are available for this feature:

BLAST of GR420933 vs. SwissProt
BLAST of GR420933 vs. TrEMBL
BLAST of GR420933 vs. TAIR peptide

BLAST of GR420933 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ALL8_OLEEU	3.542e-17	41.84	Calcium-binding allergen Ole e 8 OS=Olea europaea ...	[more]
CML27_ARATH	8.725e-16	36.43	Probable calcium-binding protein CML27 OS=Arabidop...	[more]
CML26_ARATH	2.539e-15	37.24	Probable calcium-binding protein CML26 OS=Arabidop...	[more]
CML23_ARATH	5.852e-12	36.29	Probable calcium-binding protein CML23 OS=Arabidop...	[more]
CML24_ARATH	4.954e-11	36.80	Calcium-binding protein CML24 OS=Arabidopsis thali...	[more]
CML16_ORYSJ	8.451e-11	40.63	Probable calcium-binding protein CML16 OS=Oryza sa...	[more]
CML10_ORYSJ	1.883e-10	36.84	Probable calcium-binding protein CML10 OS=Oryza sa...	[more]
CML18_ORYSJ	1.220e-9	35.97	Probable calcium-binding protein CML18 OS=Oryza sa...	[more]
POLC2_JUNOX	7.910e-9	31.67	Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2	[more]
CML8_ARATH	6.696e-8	30.25	Calmodulin-like protein 8 OS=Arabidopsis thaliana ...	[more]

BLAST of GR420933 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q93YA8_SESRO	7.143e-31	53.33	Calcium binding protein OS=Sesbania rostrata GN=cb...	[more]
C6T2C2_SOYBN	4.333e-28	56.62	Putative uncharacterized protein OS=Glycine max PE...	[more]
D3Y1S3_9FABA	2.303e-21	58.33	Calmodulin 24-like protein (Fragment) OS=Eperua gr...	[more]
D3Y1S4_9FABA	6.701e-21	57.41	Calmodulin 24-like protein (Fragment) OS=Eperua gr...	[more]
D3Y1R9_9FABA	8.752e-21	57.41	Calmodulin 24-like protein (Fragment) OS=Eperua fa...	[more]
D3Y1T6_9FABA	5.673e-20	56.31	Calmodulin 24-like protein (Fragment) OS=Tachigali...	[more]
A5ATQ5_VITVI	2.815e-19	42.38	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D3Y1T1_9FABA	3.677e-19	54.63	Calmodulin 24-like protein (Fragment) OS=Bauhinia ...	[more]
D3Y1S9_BAUPU	3.677e-19	54.37	Calmodulin 24-like protein (Fragment) OS=Bauhinia ...	[more]
B9IEB3_POPTR	5.310e-18	44.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GR420933 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G18210.2	9.097e-17	36.43	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT1G18210.1	9.097e-17	36.43	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT1G73630.1	2.647e-16	37.24	\| Symbols: \| EF hand calcium-binding protein fami...	[more]
AT1G66400.1	6.102e-13	36.29	\| Symbols: CML23 \| calmodulin like 23 \| chr1:24770...	[more]
AT5G37770.1	5.165e-12	36.80	\| Symbols: TCH2, CML24 \| EF hand calcium-binding p...	[more]
AT4G14640.1	6.981e-9	30.25	\| Symbols: CAM8, AtCML8 \| calmodulin 8 \| chr4:8397...	[more]
AT3G56800.1	3.831e-7	28.10	\| Symbols: CAM3, acam-3 \| calmodulin 3 \| chr3:2103...	[more]
AT2G27030.3	3.831e-7	28.10	\| Symbols: CAM5 \| calmodulin 5 \| chr2:11532069-115...	[more]
AT2G27030.1	3.831e-7	28.10	\| Symbols: CAM5, ACAM-2 \| calmodulin 5 \| chr2:1153...	[more]
AT2G41110.1	3.831e-7	28.10	\| Symbols: CAM2, ATCAL5 \| calmodulin 2 \| chr2:1714...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	Calcium-binding EF-hand	SMART	SM00054	no description	coord: 122..150 score: 0.0
IPR011992	EF-hand-like domain	GENE3D	1.10.238.10	no description	coord: 45..168 score: 9.3
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 131..143 score
IPR018248	EF-hand	PFAM	PF00036	efhand	coord: 122..147 score: 2.1
IPR018249	EF-HAND 2	PROFILE	PS50222	EF_HAND_2	coord: 118..153 score: 12.672coord: 154..170 score: 5
None	No IPR available	PANTHER	PTHR10891	CALMODULIN	coord: 46..168 score: 3.6
None	No IPR available	PANTHER	PTHR10891:SF39	CALMODULIN 2 RELATED	coord: 46..168 score: 3.6
None	No IPR available	SUPERFAMILY	47473	EF-hand	coord: 44..167 score: 8.1
None	No IPR available	SEG	seg	seg	coord: 11..23 score
None	No IPR available	SEG	seg	seg	coord: 53..62 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420933 ID=GR420933; Name=GR420933; organism=Cicer arietinum; type=EST; length=606bp
TTTCTAAATTTTTCATTTTCAGTTATTCCCTCCATTCACCGTAACTTCCC
TCTTTCTCTCGAAACGAACGCTAACGATGGCGACCGAACAAAACACTGAA
ACGAGCGAATCCAACCGTCATTTACTTCGAAGCACCAAACCCTCCGACAT
GGACGAAGTTAAGCGAGTTTTCACTCGCTTGACGGCAACGACGCCAACGG
CGATCTCTGTCACCGAGTTGGACAACGTCCTTCGCACTCTGAGGTCCAGC
GCGTCGGATCGGGTGTTCCACATGATTATGGAAGACCTTGACACCGACAA
AACCTCTCGGAGTTCCGACGGTTTTATTGCCGCCTTCTGCCGCTCCGGTT
CGACAGACGGCGGCGCCTCAGAGCTACGTGATGCCTTCGATCTGTACGAT
AAGGACAAGAACGGCCTCATCTCCGCAACGGAACTCTGTCAGGTGTTGAG
TGTTCCGTTGAGGAAACAGCCTCGGAATGAATGCCGCAATATGATCAAAT
CCGTTGATTCAAAACGTTAACTTCGACGACGACGGTGATGGTTCAAACAG
ATGATGTCCAATAACAGTGCCAATGCATTTTCGAGAAATTAACTAATAGA
TTTATC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR018248	EF-hand
IPR018249	EF-HAND 2

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