GR914201

EST Overview


Name	GR914201
Unique Name	GR914201
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	444

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR914201 vs. TrEMBL
Match: B9S2X3_RICCO (Ubiquitin-protein ligase, putative OS=Ricinus communis GN=RCOM_0563460 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 3.240e-39
Identity = 75/80 (93.75%), Postives = 79/80 (98.75%), Query Frame = 3

Query:   30 SPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNR 269
            SPEWKEGFTWAFD+PPKGQKLHI+CKSKNTFGKS+LGRVTIQIDKVVTEGVYSGLFSLNHD NKDGSSRTLEIEIIW+NR
Sbjct: 2013 SPEWKEGFTWAFDVPPKGQKLHIICKSKNTFGKSTLGRVTIQIDKVVTEGVYSGLFSLNHDNNKDGSSRTLEIEIIWTNR 2092

[Close]

BLAST of GR914201 vs. TrEMBL
Match: B9H7H1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831512 PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.231e-38
Identity = 73/82 (89.02%), Postives = 80/82 (97.56%), Query Frame = 3

Query:   30 SPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRIS 275
            SPEWKEGFTWAFD+PPKGQKLHI+CKSKNTFGK++LGRVTIQIDKVV+EGVYSGLFSLNHD NKDGSSRTLEIEI+W+NR S
Sbjct: 2024 SPEWKEGFTWAFDVPPKGQKLHIICKSKNTFGKNTLGRVTIQIDKVVSEGVYSGLFSLNHDSNKDGSSRTLEIEIVWTNRTS 2105

[Close]

BLAST of GR914201 vs. TrEMBL
Match: Q2R9P0_ORYSJ (Armadillo/beta-catenin-like repeat family protein, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os11g08090 PE=4 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 2.403e-34
Identity = 70/85 (82.35%), Postives = 76/85 (89.41%), Query Frame = 3

Query:   33 PEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISNDDI 287
            P W EGFTW FDIPPKGQKL+I+CKSKNTFGKS+LGRVTIQID VVTEGVYSG FSL HDG KDG SRTLEIEI+WSNR SND++
Sbjct: 2094 PVWNEGFTWLFDIPPKGQKLYILCKSKNTFGKSTLGRVTIQIDNVVTEGVYSGFFSLKHDGGKDG-SRTLEIEIVWSNRPSNDNM 2177

[Close]

BLAST of GR914201 vs. TrEMBL
Match: C5Y680_SORBI (Putative uncharacterized protein Sb05g005390 OS=Sorghum bicolor GN=Sb05g005390 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.138e-34
Identity = 69/85 (81.18%), Postives = 76/85 (89.41%), Query Frame = 3

Query:   33 PEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISNDDI 287
            P W EGFTW FD+ PKGQKL+I+CKSKNTFGKS+LGRVTIQIDKVVTEGVYSG FSL+HDG KDG SRTLEIEI+WSNR SND +
Sbjct: 2034 PAWNEGFTWLFDVAPKGQKLYIICKSKNTFGKSTLGRVTIQIDKVVTEGVYSGFFSLSHDGGKDG-SRTLEIEIVWSNRPSNDSM 2117

[Close]

BLAST of GR914201 vs. TrEMBL
Match: B8BJH0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_35372 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.098e-34
Identity = 70/83 (84.34%), Postives = 74/83 (89.16%), Query Frame = 3

Query:   33 PEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            P W EGFTW FDIPPKGQKL+I+CKSKNTFGKS+LGRVTIQID VVTEGVYSG FSL HDG KDG SRTLEIEI+WSNR SND
Sbjct: 2115 PVWNEGFTWLFDIPPKGQKLYILCKSKNTFGKSTLGRVTIQIDNVVTEGVYSGFFSLKHDGGKDG-SRTLEIEIVWSNRPSND 2196

[Close]

BLAST of GR914201 vs. TrEMBL
Match: B4FJ35_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.098e-34
Identity = 69/83 (83.13%), Postives = 75/83 (90.36%), Query Frame = 3

Query:   33 PEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            P W EGFTW FD+ PKGQKL+I+CKSKNTFGKS+LGRVTIQIDKVVTEGVYSG FSL+HDG KDG SRTLEIEI+WSNR SND
Sbjct:   88 PAWNEGFTWLFDVAPKGQKLYIICKSKNTFGKSTLGRVTIQIDKVVTEGVYSGFFSLSHDGGKDG-SRTLEIEIVWSNRPSND 169

[Close]

BLAST of GR914201 vs. TrEMBL
Match: Q9CAQ9_ARATH (Putative uncharacterized protein T5M16.5 OS=Arabidopsis thaliana GN=At1g77460 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 5.353e-34
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 3

Query:   27 TSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            T+PEWKEGFTWAFD+PPKGQKLHI+CKSK+TFGK++LGRVTIQIDKVVTEG YSG  SLNH+ +KD SSR+L+IEI WSNR +++
Sbjct: 2024 TTPEWKEGFTWAFDVPPKGQKLHIICKSKSTFGKTTLGRVTIQIDKVVTEGEYSGSLSLNHENSKDASSRSLDIEIAWSNRTTDE 2108

[Close]

BLAST of GR914201 vs. TrEMBL
Match: Q8GXS1_ARATH (Putative uncharacterized protein At1g77460/T5M16_5 OS=Arabidopsis thaliana GN=At1g77460/T5M16_5 PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 5.353e-34
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 3

Query:   27 TSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            T+PEWKEGFTWAFD+PPKGQKLHI+CKSK+TFGK++LGRVTIQIDKVVTEG YSG  SLNH+ +KD SSR+L+IEI WSNR +++
Sbjct:  348 TTPEWKEGFTWAFDVPPKGQKLHIICKSKSTFGKTTLGRVTIQIDKVVTEGEYSGSLSLNHENSKDASSRSLDIEIAWSNRTTDE 432

[Close]

BLAST of GR914201 vs. TrEMBL
Match: D7KUJ4_ARALY (C2 domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_476910 PE=4 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 9.130e-34
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 3

Query:   27 TSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            T+PEWKEGFTWAFD+PPKGQKLHI+CKSK+TFGK++LGRVTIQIDKVVTEG YSG  SLNH+ +KD SSR+L+IEI WSNR +++
Sbjct: 2049 TTPEWKEGFTWAFDVPPKGQKLHIICKSKSTFGKTTLGRVTIQIDKVVTEGEYSGSLSLNHENSKDTSSRSLDIEIAWSNRTTDE 2133

[Close]

BLAST of GR914201 vs. TrEMBL
Match: A9RP92_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_117324 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 9.151e-26
Identity = 52/80 (65.00%), Postives = 64/80 (80.00%), Query Frame = 3

Query:   30 SPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNR 269
            SP+WK+GF WAFD PPKGQKLHI CK+K  FGK SLG+VTIQID+VV +G  SG ++L  + N+DG+ RTLE+E  WSNR
Sbjct: 2053 SPQWKQGFAWAFDNPPKGQKLHIACKNKGAFGKGSLGKVTIQIDRVVMQGTISGQYTLQPETNRDGTPRTLEVEFQWSNR 2132

[Close]

BLAST of GR914201 vs. TAIR peptide
Match: AT1G77460.2 (| Symbols: | Armadillo/beta-catenin-like repeat ; C2 calcium/lipid-binding domain (CaLB) protein | chr1:29104378-29111580 FORWARD LENGTH=2136)

HSP 1 Score: 147.132 bits (370), Expect = 3.584e-36
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 3

Query:   27 TSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            T+PEWKEGFTWAFD+PPKGQKLHI+CKSK+TFGK++LGRVTIQIDKVVTEG YSG  SLNH+ +KD SSR+L+IEI WSNR +++
Sbjct: 2050 TTPEWKEGFTWAFDVPPKGQKLHIICKSKSTFGKTTLGRVTIQIDKVVTEGEYSGSLSLNHENSKDASSRSLDIEIAWSNRTTDE 2134

[Close]

BLAST of GR914201 vs. TAIR peptide
Match: AT1G77460.1 (| Symbols: | Armadillo/beta-catenin-like repeat ; C2 calcium/lipid-binding domain (CaLB) protein | chr1:29104378-29111580 FORWARD LENGTH=2136)

HSP 1 Score: 147.132 bits (370), Expect = 3.584e-36
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 3

Query:   27 TSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNRISND 281
            T+PEWKEGFTWAFD+PPKGQKLHI+CKSK+TFGK++LGRVTIQIDKVVTEG YSG  SLNH+ +KD SSR+L+IEI WSNR +++
Sbjct: 2050 TTPEWKEGFTWAFDVPPKGQKLHIICKSKSTFGKTTLGRVTIQIDKVVTEGEYSGSLSLNHENSKDASSRSLDIEIAWSNRTTDE 2134

[Close]

BLAST of GR914201 vs. TAIR peptide
Match: AT1G44120.1 (| Symbols: | Armadillo/beta-catenin-like repeat ; C2 calcium/lipid-binding domain (CaLB) protein | chr1:16780610-16787414 FORWARD LENGTH=2114)

HSP 1 Score: 105.916 bits (263), Expect = 9.158e-24
Identity = 50/85 (58.82%), Postives = 62/85 (72.94%), Query Frame = 3

Query:   18 RAGTSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSS-RTLEIEIIWSNR 269
            +  +SP WKE FTW F  PP+GQ L IVCKS N F   +LG+V I IDKV++EG YSG+F LN +  KD SS R+LEIEI+WSN+
Sbjct: 2028 KRSSSPVWKESFTWDFAAPPRGQFLEIVCKSNNIFRNKNLGKVRIPIDKVLSEGSYSGIFKLNDESKKDNSSDRSLEIEIVWSNQ 2112

[Close]

BLAST of GR914201 vs. TAIR peptide
Match: AT2G22125.1 (| Symbols: CSI1 | binding | chr2:9406793-9414223 FORWARD LENGTH=2150)

HSP 1 Score: 95.1301 bits (235), Expect = 1.616e-20
Identity = 47/82 (57.32%), Postives = 60/82 (73.17%), Query Frame = 3

Query:   24 GTSPEWKEGFTWAFDIPPKGQKLHIVCKSKNTFGKSSLGRVTIQIDKVVTEGVYSGLFSLNHDGNKDGSSRTLEIEIIWSNR 269
            G +PEW E F+W+F+ PPKGQKLHI CK+K+  GKSS G+VTIQID+VV  G  +G +SL  + +K G  R LEIE  WSN+
Sbjct: 2071 GPNPEWDESFSWSFESPPKGQKLHISCKNKSKMGKSSFGKVTIQIDRVVMLGAVAGEYSLLPE-SKSG-PRNLEIEFQWSNK 2150

The following BLAST results are available for this feature:

BLAST of GR914201 vs. TrEMBL
BLAST of GR914201 vs. TAIR peptide

BLAST of GR914201 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S2X3_RICCO	3.240e-39	93.75	Ubiquitin-protein ligase, putative OS=Ricinus comm...	[more]
B9H7H1_POPTR	1.231e-38	89.02	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q2R9P0_ORYSJ	2.403e-34	82.35	Armadillo/beta-catenin-like repeat family protein,...	[more]
C5Y680_SORBI	3.138e-34	81.18	Putative uncharacterized protein Sb05g005390 OS=So...	[more]
B8BJH0_ORYSI	4.098e-34	84.34	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FJ35_MAIZE	4.098e-34	83.13	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q9CAQ9_ARATH	5.353e-34	76.47	Putative uncharacterized protein T5M16.5 OS=Arabid...	[more]
Q8GXS1_ARATH	5.353e-34	76.47	Putative uncharacterized protein At1g77460/T5M16_5...	[more]
D7KUJ4_ARALY	9.130e-34	76.47	C2 domain-containing protein OS=Arabidopsis lyrata...	[more]
A9RP92_PHYPA	9.151e-26	65.00	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of GR914201 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G77460.2	3.584e-36	76.47	\| Symbols: \| Armadillo/beta-catenin-like repeat ;...	[more]
AT1G77460.1	3.584e-36	76.47	\| Symbols: \| Armadillo/beta-catenin-like repeat ;...	[more]
AT1G44120.1	9.158e-24	58.82	\| Symbols: \| Armadillo/beta-catenin-like repeat ;...	[more]
AT2G22125.1	1.616e-20	57.32	\| Symbols: CSI1 \| binding \| chr2:9406793-9414223 F...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 calcium-dependent membrane targeting	PFAM	PF00168	C2	coord: 9..48 score: 0.0
IPR008973	C2 calcium/lipid-binding domain, CaLB	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 8..94 score: 8.4
None	No IPR available	GENE3D	2.60.40.150	no description	coord: 9..84 score: 4.3
None	No IPR available	SEG	seg	seg	coord: 42..52 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR914201 ID=GR914201; Name=GR914201; organism=Cicer arietinum; type=EST; length=444bp
GATTTCGAGCGGCCGCCCGGGCAGGTACTTCTCCGGAATGGAAAGAAGGA
TTCACATGGGCATTTGATATACCTCCCAAGGGCCAAAAGCTCCATATCGT
ATGCAAAAGCAAGAATACTTTTGGGAAGTCAAGTCTTGGAAGAGTCACCA
TCCAGATTGATAAAGTTGTAACAGAAGGGGTTTATAGTGGATTATTCAGT
CTTAATCATGACGGCAACAAAGATGGTTCTTCCCGAACACTTGAAATTGA
GATTATATGGTCTAACAGAATTTCTAATGATGACATTTGAAAGGGGCGGT
GAGCTACTTTTCCGGCTGGAAGCATAGAAAGTAATCTGCAGTATGCATGA
TATGTTCAATATGGTTTTCTTGTAATTATCAACATGTTTGGAAGATCCAA
TTTTGTAGCCTGGCAGATAAGGTTGCCTCATTTGTAAGTGTAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR000008	C2_dom
IPR008973	C2 calcium/lipid-binding domain, CaLB

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