HO063518

EST Overview


Name	HO063518
Unique Name	HO063518
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	324

Libraries


Library Name	Type
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO063518 vs. SwissProt
Match: RH10_ARATH (DEAD-box ATP-dependent RNA helicase 10 OS=Arabidopsis thaliana GN=RH10 PE=2 SV=2)

HSP 1 Score: 97.4413 bits (241), Expect = 1.429e-40
Identity = 47/51 (92.16%), Postives = 48/51 (94.12%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KLQR CLRNPVKIEAASKYSTVDTLKQQYRFV AK+KDCYL
Sbjct:  201 FSATMTKKVRKLQRACLRNPVKIEAASKYSTVDTLKQQYRFVAAKYKDCYL 251

HSP 2 Score: 88.5817 bits (218), Expect = 1.429e-40
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+ DH+ +TKGFSL  LKYLVLDEADRLLNEDFE+SLN+IL  IP ER+TFLF
Sbjct:  146 TPGRLWDHMSDTKGFSLKSLKYLVLDEADRLLNEDFEKSLNQILEEIPLERKTFLF 201

[Close]

BLAST of HO063518 vs. SwissProt
Match: RH10_ORYSJ (DEAD-box ATP-dependent RNA helicase 10 OS=Oryza sativa subsp. japonica GN=Os03g0669000 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.268e-38
Identity = 41/57 (71.93%), Postives = 53/57 (92.98%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+LDHL +TKGFSL +LKYLVLDEAD+LLN +F+++L++IL +IP+ERRTFLF
Sbjct:  167 GTPGRLLDHLTDTKGFSLNKLKYLVLDEADKLLNVEFQKALDDILNVIPKERRTFLF 223

HSP 2 Score: 86.2705 bits (212), Expect = 6.268e-38
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR CLRNPVK+E ASKYSTVDTL+Q++ FVPA +KDC+L
Sbjct:  223 FSATMTNKVSKLQRACLRNPVKVEVASKYSTVDTLRQEFYFVPADYKDCFL 273

[Close]

BLAST of HO063518 vs. SwissProt
Match: DDX47_DICDI (Probable ATP-dependent RNA helicase ddx47 OS=Dictyostelium discoideum GN=ddx47 PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.552e-32
Identity = 35/57 (61.40%), Postives = 47/57 (82.46%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            G+PGRVL HL++TKGF+L  +KY ++DEADRL + DFEE +N IL +IP+ER T+LF
Sbjct:  243 GSPGRVLHHLEHTKGFNLRSIKYFIMDEADRLFSADFEEEVNNILKVIPKERNTYLF 299

HSP 2 Score: 78.9518 bits (193), Expect = 1.552e-32
Identity = 38/51 (74.51%), Postives = 41/51 (80.39%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR  L NPVK++ ASKY TVDTL QQY FVP K+KDCYL
Sbjct:  299 FSATMTSKVAKLQRASLVNPVKVQVASKYQTVDTLLQQYLFVPFKYKDCYL 349

[Close]

BLAST of HO063518 vs. SwissProt
Match: DDX47_BOVIN (Probable ATP-dependent RNA helicase DDX47 OS=Bos taurus GN=DDX47 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 9.880e-32
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+NTKGF+L  LKYLV+DEADR+LN DFE  +++IL +IPR+R+TFLF
Sbjct:  151 TPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLF 206

HSP 2 Score: 71.2478 bits (173), Expect = 9.880e-32
Identity = 33/51 (64.71%), Postives = 40/51 (78.43%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV+KLQR  L+NPVK   +SKY TV+ L+Q Y F+P+K KD YL
Sbjct:  206 FSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYLFIPSKFKDTYL 256

[Close]

BLAST of HO063518 vs. SwissProt
Match: DDX47_MOUSE (Probable ATP-dependent RNA helicase DDX47 OS=Mus musculus GN=Ddx47 PE=2 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 9.880e-32
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+NTKGF+L  LKYLV+DEADR+LN DFE  +++IL +IPR+R+TFLF
Sbjct:  149 TPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLF 204

HSP 2 Score: 71.2478 bits (173), Expect = 9.880e-32
Identity = 33/51 (64.71%), Postives = 40/51 (78.43%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV+KLQR  L+NPVK   +SKY TV+ L+Q Y F+P+K KD YL
Sbjct:  204 FSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYLFIPSKFKDTYL 254

[Close]

BLAST of HO063518 vs. SwissProt
Match: DDX47_HUMAN (Probable ATP-dependent RNA helicase DDX47 OS=Homo sapiens GN=DDX47 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.287e-31
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+NTKGF+L  LKYLV+DEADR+LN DFE  +++IL +IPR+R+TFLF
Sbjct:  149 TPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLF 204

HSP 2 Score: 70.8626 bits (172), Expect = 1.287e-31
Identity = 33/51 (64.71%), Postives = 40/51 (78.43%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV+KLQR  L+NPVK   +SKY TV+ L+Q Y F+P+K KD YL
Sbjct:  204 FSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYL 254

[Close]

BLAST of HO063518 vs. SwissProt
Match: DDX47_CAEEL (Putative ATP-dependent RNA helicase T26G10.1 OS=Caenorhabditis elegans GN=T26G10.1 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 8.159e-31
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+NTKGF+L  LK+L++DEADR+LN DFE  L++IL +IPRERRT+LF
Sbjct:  169 TPGRLVDHLENTKGFNLKALKFLIMDEADRILNMDFEVELDKILKVIPRERRTYLF 224

HSP 2 Score: 68.5514 bits (166), Expect = 8.159e-31
Identity = 31/51 (60.78%), Postives = 39/51 (76.47%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KL+R  LR+P ++  +S+Y TVD LKQ Y FVP K+K+ YL
Sbjct:  224 FSATMTKKVSKLERASLRDPARVSVSSRYKTVDNLKQHYIFVPNKYKETYL 274

[Close]

BLAST of HO063518 vs. SwissProt
Match: RRP3_SCHPO (ATP-dependent rRNA helicase rrp3 OS=Schizosaccharomyces pombe GN=rrp3 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.347e-30
Identity = 37/56 (66.07%), Postives = 47/56 (83.93%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+NTKGFSL  LKYL++DEADRLL+ DF   +++IL +IP ERRT LF
Sbjct:  171 TPGRLMDHLENTKGFSLKNLKYLIMDEADRLLDMDFGPIIDKILKIIPHERRTLLF 226

HSP 2 Score: 71.2478 bits (173), Expect = 2.347e-30
Identity = 34/51 (66.67%), Postives = 39/51 (76.47%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KVEKLQR  L  PV++  +SK+STVDTL Q+Y F P KHKD YL
Sbjct:  226 FSATMTSKVEKLQRASLHQPVRVAVSSKFSTVDTLIQRYLFFPFKHKDTYL 276

[Close]

BLAST of HO063518 vs. SwissProt
Match: RRP3_USTMA (ATP-dependent rRNA helicase RRP3 OS=Ustilago maydis GN=RRP3 PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.277e-29
Identity = 37/56 (66.07%), Postives = 46/56 (82.14%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+ DHL+NTKGFSL  L+YLV+DEADRLL+ DF   ++++L  IPRERRT LF
Sbjct:  229 TPGRLQDHLENTKGFSLRGLQYLVMDEADRLLDMDFGPIIDKLLQSIPRERRTMLF 284

HSP 2 Score: 71.2478 bits (173), Expect = 3.277e-29
Identity = 33/51 (64.71%), Postives = 39/51 (76.47%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR  L+NPV++E  +KY+TV TLKQ Y F+P  HKD YL
Sbjct:  284 FSATMTTKVAKLQRASLKNPVRVEVDTKYTTVSTLKQHYMFMPFAHKDTYL 334

[Close]

BLAST of HO063518 vs. SwissProt
Match: RRP3_ASPOR (ATP-dependent rRNA helicase rrp3 OS=Aspergillus oryzae GN=rrp3 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 3.284e-29
Identity = 40/56 (71.43%), Postives = 49/56 (87.50%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+LDHL+NTKGFSL  LKYLV+DEADRLL+ DF   L++IL ++PRERRT+LF
Sbjct:  177 TPGRLLDHLENTKGFSLRNLKYLVMDEADRLLDMDFGPILDKILKVLPRERRTYLF 232

HSP 2 Score: 63.5438 bits (153), Expect = 3.284e-29
Identity = 30/52 (57.69%), Postives = 40/52 (76.92%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIE-AASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATM+ KVE LQR  L+NP+++  ++SK+ TV TL+Q Y F+P KHKD YL
Sbjct:  232 FSATMSSKVESLQRASLQNPLRVAVSSSKFQTVSTLQQSYIFIPHKHKDLYL 283

[Close]

BLAST of HO063518 vs. TrEMBL
Match: Q2PEX8_TRIPR (Putative replication protein A1 OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 4.316e-47
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGRVLDHLKNTKGFSL +LKYLVLDEADRLLNEDFEESLNEILG+IPRERRTFLF
Sbjct:  135 GTPGRVLDHLKNTKGFSLSKLKYLVLDEADRLLNEDFEESLNEILGMIPRERRTFLF 191

HSP 2 Score: 99.7525 bits (247), Expect = 4.316e-47
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKVEKLQRVCLRNPVKIEA++KYSTVDTLKQQYRF+PAK KDCYL
Sbjct:  191 FSATMTKKVEKLQRVCLRNPVKIEASTKYSTVDTLKQQYRFLPAKRKDCYL 241

[Close]

BLAST of HO063518 vs. TrEMBL
Match: B9RI04_RICCO (Dead box ATP-dependent RNA helicase, putative OS=Ricinus communis GN=RCOM_1574790 PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.243e-42
Identity = 46/51 (90.20%), Postives = 50/51 (98.04%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV+KLQR CLRNPVKIEAASKYSTVDTLKQ+YRF+PAK+KDCYL
Sbjct:  193 FSATMTKKVKKLQRACLRNPVKIEAASKYSTVDTLKQEYRFIPAKYKDCYL 243

HSP 2 Score: 97.0561 bits (240), Expect = 2.243e-42
Identity = 47/56 (83.93%), Postives = 52/56 (92.86%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL NTKGFSL  LKYLVLDEADRLLNEDFE+SL+EIL +IPRERRTFLF
Sbjct:  138 TPGRLVDHLSNTKGFSLRTLKYLVLDEADRLLNEDFEKSLDEILKVIPRERRTFLF 193

[Close]

BLAST of HO063518 vs. TrEMBL
Match: B9HGU2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_871931 PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.922e-42
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV+KLQR CLRNPVKIEAASKYSTVDTLKQQYRFVP+KHKDCYL
Sbjct:  210 FSATMTKKVKKLQRACLRNPVKIEAASKYSTVDTLKQQYRFVPSKHKDCYL 260

HSP 2 Score: 93.9745 bits (232), Expect = 2.922e-42
Identity = 44/56 (78.57%), Postives = 52/56 (92.86%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+LDHL NTKGFSL  LKYLVLDEADRLLNE+FE+SL+EIL +IPR+R+T+LF
Sbjct:  155 TPGRLLDHLSNTKGFSLRTLKYLVLDEADRLLNEEFEKSLDEILNVIPRDRKTYLF 210

[Close]

BLAST of HO063518 vs. TrEMBL
Match: D7T0S6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_65.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032335001 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 5.365e-41
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KLQR CLRNPVKIE ASKYSTVDTLKQQYRFVPAK+K+CYL
Sbjct:  189 FSATMTKKVRKLQRACLRNPVKIEVASKYSTVDTLKQQYRFVPAKYKECYL 239

HSP 2 Score: 94.3597 bits (233), Expect = 5.365e-41
Identity = 42/57 (73.68%), Postives = 53/57 (92.98%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR++DHL NTKGFSL  +KYLVLDEADRLLN+DFE++++EIL +IPRER+T+LF
Sbjct:  133 GTPGRLMDHLSNTKGFSLRTMKYLVLDEADRLLNDDFEKAIDEILSVIPRERKTYLF 189

[Close]

BLAST of HO063518 vs. TrEMBL
Match: D7MUG3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_919338 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.465e-40
Identity = 47/51 (92.16%), Postives = 48/51 (94.12%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KLQR CLRNPVKIEAASKYSTVDTLKQQYRFV AK+KDCYL
Sbjct:  201 FSATMTKKVRKLQRACLRNPVKIEAASKYSTVDTLKQQYRFVAAKYKDCYL 251

HSP 2 Score: 91.2781 bits (225), Expect = 4.465e-40
Identity = 44/56 (78.57%), Postives = 50/56 (89.29%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+ DH+ +TKGFSL  LKYLVLDEADRLLNEDFE+SLN+IL  IPRER+TFLF
Sbjct:  146 TPGRLWDHMSDTKGFSLKSLKYLVLDEADRLLNEDFEKSLNQILEEIPRERKTFLF 201

[Close]

BLAST of HO063518 vs. TrEMBL
Match: D7TAH1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_10.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010258001 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.358e-37
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KLQR CLRNPVKIEA SKYSTVDTLKQQYRFVPAK+K+CYL
Sbjct:   51 FSATMTKKVRKLQRACLRNPVKIEAGSKYSTVDTLKQQYRFVPAKYKECYL 101

HSP 2 Score: 81.2629 bits (199), Expect = 4.358e-37
Identity = 36/51 (70.59%), Postives = 47/51 (92.16%), Query Frame = -2

Query:  153 LDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 305
            +DHL NTKGFSL  +KYLVLDEADRLLN+DFE++++EIL +IP+ER+T+LF
Sbjct:    1 MDHLSNTKGFSLRTMKYLVLDEADRLLNDDFEKAIDEILSVIPQERKTYLF 51

[Close]

BLAST of HO063518 vs. TrEMBL
Match: A9RTN8_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_178010 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.359e-37
Identity = 42/57 (73.68%), Postives = 51/57 (89.47%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR++DHL NTKGFSL  +KYLVLDEADRLLN DFE+ ++EIL +IP+ERRT+LF
Sbjct:  143 GTPGRLVDHLTNTKGFSLRTIKYLVLDEADRLLNMDFEQEIDEILKVIPKERRTYLF 199

HSP 2 Score: 86.6557 bits (213), Expect = 7.359e-37
Identity = 38/51 (74.51%), Postives = 46/51 (90.20%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR CL+NPVK+E ++KYSTVD+LKQ+Y F+PAK+KDCYL
Sbjct:  199 FSATMTTKVAKLQRACLKNPVKVEVSAKYSTVDSLKQEYLFIPAKYKDCYL 249

[Close]

BLAST of HO063518 vs. TrEMBL
Match: B7EDZ1_ORYSJ (cDNA clone:J013120J20, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.248e-36
Identity = 41/57 (71.93%), Postives = 53/57 (92.98%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+LDHL +TKGFSL +LKYLVLDEAD+LLN +F+++L++IL +IP+ERRTFLF
Sbjct:  167 GTPGRLLDHLTDTKGFSLNKLKYLVLDEADKLLNVEFQKALDDILNVIPKERRTFLF 223

HSP 2 Score: 86.2705 bits (212), Expect = 1.248e-36
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR CLRNPVK+E ASKYSTVDTL+Q++ FVPA +KDC+L
Sbjct:  223 FSATMTNKVSKLQRACLRNPVKVEVASKYSTVDTLRQEFYFVPADYKDCFL 273

[Close]

BLAST of HO063518 vs. TrEMBL
Match: A3AL75_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_12029 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.248e-36
Identity = 41/57 (71.93%), Postives = 53/57 (92.98%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+LDHL +TKGFSL +LKYLVLDEAD+LLN +F+++L++IL +IP+ERRTFLF
Sbjct:  167 GTPGRLLDHLTDTKGFSLNKLKYLVLDEADKLLNVEFQKALDDILNVIPKERRTFLF 223

HSP 2 Score: 86.2705 bits (212), Expect = 1.248e-36
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR CLRNPVK+E ASKYSTVDTL+Q++ FVPA +KDC+L
Sbjct:  223 FSATMTNKVSKLQRACLRNPVKVEVASKYSTVDTLRQEFYFVPADYKDCFL 273

[Close]

BLAST of HO063518 vs. TrEMBL
Match: A2XKG2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_12939 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.248e-36
Identity = 41/57 (71.93%), Postives = 53/57 (92.98%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+LDHL +TKGFSL +LKYLVLDEAD+LLN +F+++L++IL +IP+ERRTFLF
Sbjct:  167 GTPGRLLDHLTDTKGFSLNKLKYLVLDEADKLLNVEFQKALDDILNVIPKERRTFLF 223

HSP 2 Score: 86.2705 bits (212), Expect = 1.248e-36
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMT KV KLQR CLRNPVK+E ASKYSTVDTL+Q++ FVPA +KDC+L
Sbjct:  223 FSATMTNKVSKLQRACLRNPVKVEVASKYSTVDTLRQEFYFVPADYKDCFL 273

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT5G60990.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr5:24546601-24549148 REVERSE LENGTH=456)

HSP 1 Score: 97.4413 bits (241), Expect = 1.132e-41
Identity = 47/51 (92.16%), Postives = 48/51 (94.12%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSATMTKKV KLQR CLRNPVKIEAASKYSTVDTLKQQYRFV AK+KDCYL
Sbjct:  201 FSATMTKKVRKLQRACLRNPVKIEAASKYSTVDTLKQQYRFVAAKYKDCYL 251

HSP 2 Score: 88.5817 bits (218), Expect = 1.132e-41
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+ DH+ +TKGFSL  LKYLVLDEADRLLNEDFE+SLN+IL  IP ER+TFLF
Sbjct:  146 TPGRLWDHMSDTKGFSLKSLKYLVLDEADRLLNEDFEKSLNQILEEIPLERKTFLF 201

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT5G65900.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr5:26358328-26361244 FORWARD LENGTH=633)

HSP 1 Score: 75.8702 bits (185), Expect = 2.441e-19
Identity = 34/56 (60.71%), Postives = 45/56 (80.36%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+LDHL+NT GF    LK+LV+DEADR+L ++FEE L +IL L+P+ R+T LF
Sbjct:  283 TPGRLLDHLENTNGFIFKNLKFLVMDEADRILEQNFEEDLKKILNLLPKTRQTSLF 338

HSP 2 Score: 35.039 bits (79), Expect = 2.441e-19
Identity = 23/55 (41.82%), Postives = 31/55 (56.36%), Query Frame = -3

Query:    2 SFFSATMTKKVEKLQRVCLRNPVKIEA--ASKYSTVDTLKQQYRFVPAKHKDCYL 160
            S FSAT + KVE L RV L +PV I+     K  T + L+Q Y  VP+  +  +L
Sbjct:  336 SLFSATQSAKVEDLARVSLTSPVYIDVDEGRKEVTNEGLEQGYCVVPSAMRLLFL 390

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT3G18600.1 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr3:6399724-6403007 REVERSE LENGTH=568)

HSP 1 Score: 74.3294 bits (181), Expect = 5.376e-19
Identity = 34/56 (60.71%), Postives = 45/56 (80.36%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+LDHL+NTK F    LK LV+DEADR+L E+FEE +N+IL ++P+ R+T LF
Sbjct:  218 TPGRLLDHLQNTKAFIYKHLKCLVIDEADRILEENFEEDMNKILKILPKTRQTALF 273

HSP 2 Score: 35.4242 bits (80), Expect = 5.376e-19
Identity = 20/51 (39.22%), Postives = 30/51 (58.82%), Query Frame = -3

Query:   14 SFFSATMTKKVEKLQRVCLRNPVKIEA--ASKYSTVDTLKQQYRFVPAKHK 160
            + FSAT T KV+ L RV L +PV ++     +  T + L+Q Y  VP+K +
Sbjct:  271 ALFSATQTSKVKDLARVSLTSPVHVDVDDGRRKVTNEGLEQGYCVVPSKQR 321

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT5G54910.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr5:22298668-22301719 REVERSE LENGTH=739)

HSP 1 Score: 54.6842 bits (130), Expect = 2.580e-13
Identity = 24/55 (43.64%), Postives = 37/55 (67.27%), Query Frame = -2

Query:  153 PGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 317
            PGR+L H+  T  F   +L+ L+LDEADR+L+  F+  L+ I+  +P+ R+T LF
Sbjct:  201 PGRLLQHMDETPNFECPQLQILILDEADRVLDSAFKGQLDPIISQLPKHRQTLLF 255

HSP 2 Score: 35.8094 bits (81), Expect = 2.580e-13
Identity = 20/49 (40.82%), Postives = 29/49 (59.18%), Query Frame = -3

Query:   14 FSATMTKKVEKLQRVCLRNP--VKIEAASKYSTVDTLKQQYRFVPAKHK 154
            FSAT TKKV+ L R+ LR+P  + + A +  +T  +L Q    VP + K
Sbjct:  255 FSATQTKKVKDLARLSLRDPEYISVHAEAVTATPTSLMQTVMIVPVEKK 303

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT4G16630.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr4:9362176-9366449 REVERSE LENGTH=789)

HSP 1 Score: 59.3066 bits (142), Expect = 1.232e-12
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++DHL+N+    L  L  L+LDEADRLL   F   + E++ L P+ R+T LF
Sbjct:  295 TPGRMIDHLRNSMSVDLDDLAVLILDEADRLLQTGFATEITELVRLCPKRRQTMLF 350

HSP 2 Score: 28.8758 bits (63), Expect = 1.232e-12
Identity = 12/31 (38.71%), Postives = 22/31 (70.97%), Query Frame = -3

Query:   80 RGEHSFFSATMTKKVEKLQRVCLRNPVKIEA 172
            R +   FSATMT++V++L ++ L  P+++ A
Sbjct:  344 RRQTMLFSATMTEEVKELVKLSLNKPLRLSA 374

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT5G05450.1 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr5:1612077-1615195 FORWARD LENGTH=593)

HSP 1 Score: 53.1434 bits (126), Expect = 2.714e-12
Identity = 25/57 (43.86%), Postives = 37/57 (64.91%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+ D ++  +      L+ L+LDEADRLL   F+  +N I+  +P++RRT LF
Sbjct:  148 GTPGRLSDIMERMEILDFRNLEILILDEADRLLEMGFQRQVNYIISRLPKQRRTGLF 204

HSP 2 Score: 33.8834 bits (76), Expect = 2.714e-12
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = -3

Query:   71 FSATMTKKVEKLQRVCLRNPVKIEAASK 154
            FSAT T+ VE+L +  LRNPV++E  +K
Sbjct:  204 FSATQTEGVEELAKAGLRNPVRVEVRAK 231

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT1G71280.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr1:26870262-26872152 REVERSE LENGTH=465)

HSP 1 Score: 56.225 bits (134), Expect = 4.795e-11
Identity = 26/57 (45.61%), Postives = 39/57 (68.42%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+ D +K  +      L+ L+LDEADRLL+  F++ +N I+  +P++RRT LF
Sbjct:  141 GTPGRLSDMMKRMEFLDFRNLEILILDEADRLLDMGFQKQVNYIISRLPKQRRTGLF 197

HSP 2 Score: 26.5646 bits (57), Expect = 4.795e-11
Identity = 16/31 (51.61%), Postives = 19/31 (61.29%), Query Frame = -3

Query:   65 FSATMTKKVEKLQRVCLRNP-VKIEAASKYS 154
            FSAT T+ V  L +  LRNP +K EA  K S
Sbjct:  197 FSATQTQAVADLAKAGLRNPYLKCEADQKSS 227

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT5G63630.1 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr5:25472598-25476402 REVERSE LENGTH=788)

HSP 1 Score: 62.7734 bits (151), Expect = 5.153e-11
Identity = 30/58 (51.72%), Postives = 42/58 (72.41%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFS--LGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR+ DH++NT GF+  L  +K LVLDEAD LL+  F + +  I+  +P+ER+TFLF
Sbjct:  455 TPGRLKDHIENTPGFATRLKGVKVLVLDEADHLLDMGFRKDIERIISAVPKERQTFLF 512

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT2G47330.1 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr2:19429083-19431617 REVERSE LENGTH=760)

HSP 1 Score: 51.6026 bits (122), Expect = 7.952e-11
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = -2

Query:  153 TPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 320
            TPGR++D LK  K  ++ R  YLVLDEADR+ +  FE  +  I+G I  +R+T LF
Sbjct:  358 TPGRLIDMLK-MKALTMMRASYLVLDEADRMFDLGFEPQVRSIVGQIRPDRQTLLF 412

HSP 2 Score: 30.4166 bits (67), Expect = 7.952e-11
Identity = 15/44 (34.09%), Postives = 23/44 (52.27%), Query Frame = -3

Query:   23 FSATMTKKVEKLQRVCLRNPVKIEAASKYSTVDTLKQQYRFVPA 154
            FSATM  KVEKL R  L +P+++         + + Q    +P+
Sbjct:  412 FSATMPWKVEKLAREILSDPIRVTVGEVGMANEDITQVVNVIPS 455

[Close]

BLAST of HO063518 vs. TAIR peptide
Match: AT1G71370.1 (| Symbols: | DEA(D/H)-box RNA helicase family protein | chr1:26897235-26899381 REVERSE LENGTH=558)

HSP 1 Score: 56.225 bits (134), Expect = 1.041e-10
Identity = 26/57 (45.61%), Postives = 39/57 (68.42%), Query Frame = -2

Query:  153 GTPGRVLDHLKNTKGFSLGRLKYLVLDEADRLLNEDFEESLNEILGLIPRERRTFLF 323
            GTPGR+ D +K  +      L+ L+LDEADRLL+  F++ +N I+  +P++RRT LF
Sbjct:  148 GTPGRLSDMMKRMEFLDFRNLEILILDEADRLLDMGFQKQVNYIISRLPKQRRTGLF 204

HSP 2 Score: 25.409 bits (54), Expect = 1.041e-10
Identity = 19/53 (35.85%), Postives = 25/53 (47.17%), Query Frame = -3

Query:    2 FSATMTKKVEKLQRVCLRNPVKI--EAASKYSTVDTLKQQYRFVPAKHKDCYL 154
            FSAT T+ V  L +  LRN +++   A SK  T   L  +Y    A  K   L
Sbjct:  204 FSATQTQAVADLAKAGLRNAMEVISGAESKSKTSSGLYCEYLKCEADQKSSQL 256

The following BLAST results are available for this feature:

BLAST of HO063518 vs. SwissProt
BLAST of HO063518 vs. TrEMBL
BLAST of HO063518 vs. TAIR peptide

BLAST of HO063518 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RH10_ARATH	1.429e-40	92.16	DEAD-box ATP-dependent RNA helicase 10 OS=Arabidop...	[more]
RH10_ORYSJ	6.268e-38	71.93	DEAD-box ATP-dependent RNA helicase 10 OS=Oryza sa...	[more]
DDX47_DICDI	1.552e-32	61.40	Probable ATP-dependent RNA helicase ddx47 OS=Dicty...	[more]
DDX47_BOVIN	9.880e-32	67.86	Probable ATP-dependent RNA helicase DDX47 OS=Bos t...	[more]
DDX47_MOUSE	9.880e-32	67.86	Probable ATP-dependent RNA helicase DDX47 OS=Mus m...	[more]
DDX47_HUMAN	1.287e-31	67.86	Probable ATP-dependent RNA helicase DDX47 OS=Homo ...	[more]
DDX47_CAEEL	8.159e-31	67.86	Putative ATP-dependent RNA helicase T26G10.1 OS=Ca...	[more]
RRP3_SCHPO	2.347e-30	66.07	ATP-dependent rRNA helicase rrp3 OS=Schizosaccharo...	[more]
RRP3_USTMA	3.277e-29	66.07	ATP-dependent rRNA helicase RRP3 OS=Ustilago maydi...	[more]
RRP3_ASPOR	3.284e-29	71.43	ATP-dependent rRNA helicase rrp3 OS=Aspergillus or...	[more]

BLAST of HO063518 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2PEX8_TRIPR	4.316e-47	94.74	Putative replication protein A1 OS=Trifolium prate...	[more]
B9RI04_RICCO	2.243e-42	90.20	Dead box ATP-dependent RNA helicase, putative OS=R...	[more]
B9HGU2_POPTR	2.922e-42	94.12	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T0S6_VITVI	5.365e-41	90.20	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7MUG3_ARALY	4.465e-40	92.16	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TAH1_VITVI	4.358e-37	90.20	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9RTN8_PHYPA	7.359e-37	73.68	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
B7EDZ1_ORYSJ	1.248e-36	71.93	cDNA clone:J013120J20, full insert sequence OS=Ory...	[more]
A3AL75_ORYSJ	1.248e-36	71.93	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2XKG2_ORYSI	1.248e-36	71.93	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of HO063518 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G60990.1	1.132e-41	92.16	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]
AT5G65900.1	2.441e-19	60.71	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]
AT3G18600.1	5.376e-19	60.71	\| Symbols: \| P-loop containing nucleoside triphos...	[more]
AT5G54910.1	2.580e-13	43.64	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]
AT4G16630.1	1.232e-12	48.21	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]
AT5G05450.1	2.714e-12	43.86	\| Symbols: \| P-loop containing nucleoside triphos...	[more]
AT1G71280.1	4.795e-11	45.61	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]
AT5G63630.1	5.153e-11	51.72	\| Symbols: \| P-loop containing nucleoside triphos...	[more]
AT2G47330.1	7.952e-11	48.21	\| Symbols: \| P-loop containing nucleoside triphos...	[more]
AT1G71370.1	1.041e-10	45.61	\| Symbols: \| DEA(D/H)-box RNA helicase family pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 17..33 score
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 9..40 score: 0.0
None	No IPR available	PANTHER	PTHR10967	DEAD BOX ATP-DEPENDENT RNA HELICASE	coord: 10..60 score: 6.7
None	No IPR available	PANTHER	PTHR10967:SF11	DEAD-BOX PROTEIN 47	coord: 10..60 score: 6.7
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 1..57 score: 8.9
None	No IPR available	PANTHER	PTHR10967	DEAD BOX ATP-DEPENDENT RNA HELICASE	coord: 2..57 score: 2.1
None	No IPR available	PANTHER	PTHR10967:SF11	DEAD-BOX PROTEIN 47	coord: 2..57 score: 2.1
None	No IPR available	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 1..57 score: 1.1
IPR000629	RNA helicase, ATP-dependent, DEAD-box, conserved site	PROSITE	PS00039	DEAD_ATP_HELICASE	coord: 25..33 score
IPR011545	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	PFAM	PF00270	DEAD	coord: 1..57 score: 2.8
IPR014021	Helicase, superfamily 1/2, ATP-binding domain	PROFILE	PS51192	HELICASE_ATP_BIND_1	coord: 1..57 score: 10

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063518 ID=HO063518; Name=HO063518; organism=Cicer arietinum; type=EST; length=324bp
CAAGGTAGCAATCCTTGTGTTTTGCAGGCACAAAGCGATACTGTTGCTTC
AGTGTGTCCACAGTAGAATACTTGGATGCTGCTTCAATCTTCACAGGATT
CCTTAAACAAACCCTTTGTAGCTTCTCGACCTTTTTAGTCATTGTAGCAG
AAAAAAAGGAATGTTCTCCTCTCGCGAGGGATCAATCCTAAAATCTCATT
AAGTGATTCCTCAAAATCCTCATTCAATAGCCTGTCTGCCTCATCTAAGA
CCAAGTATTTTAATCTACCAAGAGAAAATCCTTTGGTGTTTTTTAGGTGA
TCCAACACCCGTCCAGGAGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0005524	ATP binding
GO:0008026	ATP-dependent helicase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000629	RNA-helicase_DEAD-box_CS
IPR011545	DEAD/DEAH_box_helicase_dom
IPR014001	Helicase_ATP-bd

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