ES560392

EST Overview
NameES560392
Unique NameES560392
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length342
Libraries
Library NameType
LIBEST_021248 Ascochyta rabiei induced librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
BLAST of ES560392 vs. SwissProt
Match: TRL31_ARATH (Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana GN=WCRKC1 PE=2 SV=3)

HSP 1 Score: 121.324 bits (303), Expect = 1.503e-27
Identity = 56/107 (52.34%), Postives = 79/107 (73.83%), Query Frame = 1
Query:   22 FCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            FC   Y   KL  S  +++       +R++A W ++++P+++EME IN+ E+LD +L HA+  SQPI+I+WMA+WCRKCIYLKPKLEKLAAE++ + KFYYVDV KV
Sbjct:   62 FCVSVYSNEKLGRSHMEKE-------WRLKAFWSNIAQPTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAKFYYVDVNKV 161          
BLAST of ES560392 vs. SwissProt
Match: TRL31_ORYSJ (Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica GN=Os02g0774100 PE=2 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 9.768e-19
Identity = 46/80 (57.50%), Postives = 59/80 (73.75%), Query Frame = 1
Query:  103 RVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            R EA + D+S+P  VEME I+  E+LD  L  +  + QPI+IDWMA+WCRKCIYLKP+LEK+A EF    +FY+VDV KV
Sbjct:   68 RAEAYFWDVSKP--VEMEEIDSMEKLDDALRWSVENKQPIIIDWMASWCRKCIYLKPRLEKIAGEF-PGVRFYFVDVNKV 144          
BLAST of ES560392 vs. SwissProt
Match: TRL32_ARATH (Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana GN=WCRKC2 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.011e-15
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 1
Query:  136 PSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            P SVE+  I      DQ++  AQ   + ++I WMAAWCRKCIYLKPKLEKLAAEF  + +FY+VDV  V
Sbjct:   73 PVSVELGPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDVNAV 141          
BLAST of ES560392 vs. TrEMBL
Match: B7FH98_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.475e-44
Identity = 90/114 (78.95%), Postives = 99/114 (86.84%), Query Frame = 1
Query:    1 ASGRGYLFCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            ASGR  L CRKS FGN +   R ++Q KI+ +N RVEAMWPDLSRPSSVEMEAI+DSEQLDQIL+HAQ +S+PILIDWMAAWCRKCIYLKPKLEKLAAEFDTK KFYYVDV KV
Sbjct:   28 ASGRDSLLCRKSCFGNII---RIEKQKKIRRKNLRVEAMWPDLSRPSSVEMEAIHDSEQLDQILVHAQQNSEPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKAKFYYVDVNKV 138          
BLAST of ES560392 vs. TrEMBL
Match: C6TFV5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.604e-41
Identity = 84/113 (74.34%), Postives = 89/113 (78.76%), Query Frame = 1
Query:    4 SGRGYLFCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            SGRGY F  KS  GN    SR  E   IK RNFRVEAMWPDLSRPS +EME INDSEQLDQIL+HAQ +SQPILIDWMA WCRKCIYLKPKLEKLA E+  K KFY+VDV KV
Sbjct:   28 SGRGYFFSHKSGCGNIWVGSRKSEGKIIKRRNFRVEAMWPDLSRPSELEMEEINDSEQLDQILVHAQQNSQPILIDWMATWCRKCIYLKPKLEKLAPEYQDKVKFYFVDVNKV 140          
BLAST of ES560392 vs. TrEMBL
Match: B9T802_RICCO (Electron transporter, putative OS=Ricinus communis GN=RCOM_0446230 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.692e-32
Identity = 67/93 (72.04%), Postives = 76/93 (81.72%), Query Frame = 1
Query:   64 RNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            + +   KI  R+ RVEA WPD++RP+S+EME INDS+QLDQILL AQ  SQP+LIDWMAAWCRKCIYLKPKLEKLA EFDTK K Y VDV KV
Sbjct:   53 KTRSFKKISKRDLRVEAFWPDMTRPTSIEMEPINDSDQLDQILLKAQELSQPVLIDWMAAWCRKCIYLKPKLEKLAFEFDTKIKIYSVDVNKV 145          
BLAST of ES560392 vs. TrEMBL
Match: B9IIA5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_576387 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 7.108e-31
Identity = 67/113 (59.29%), Postives = 83/113 (73.45%), Query Frame = 1
Query:    7 GRGYLFCRKSYFGNKLYESRNKEQ-NKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            G   L  +++ FG   ++ RNK+   K   R+ +V A WP ++RP+SVEME I+DS  LD+ILL A   SQPI+IDWMA+WCRKCIYLKPKLEKLAAE+DTK KFY VDV KV
Sbjct:   33 GSSLLLSQRTSFGCSWFDGRNKDLCKKNSKRDLKVAASWPGMTRPTSVEMEPIDDSHHLDKILLQAHELSQPIIIDWMASWCRKCIYLKPKLEKLAAEYDTKIKFYCVDVNKV 145          
BLAST of ES560392 vs. TrEMBL
Match: D7LZT0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487479 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.387e-26
Identity = 57/107 (53.27%), Postives = 78/107 (72.90%), Query Frame = 1
Query:   22 FCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            FC   Y   KL  S  +++ ++K       A W ++S+P+++EME IN+ E+LD +L HA+  SQPI+I+WMA+WCRKCIYLKPKLEKLAAE++ + KFYYVDV KV
Sbjct:   37 FCVSVYNNEKLGRSHMEKERRLK-------AFWSNISQPTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAKFYYVDVNKV 136          
BLAST of ES560392 vs. TrEMBL
Match: Q2V396_ARATH (Putative uncharacterized protein At5g06690.2 OS=Arabidopsis thaliana GN=At5g06690 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.090e-26
Identity = 56/107 (52.34%), Postives = 79/107 (73.83%), Query Frame = 1
Query:   22 FCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            FC   Y   KL  S  +++       +R++A W ++++P+++EME IN+ E+LD +L HA+  SQPI+I+WMA+WCRKCIYLKPKLEKLAAE++ + KFYYVDV KV
Sbjct:   62 FCVSVYSNEKLGRSHMEKE-------WRLKAFWSNIAQPTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAKFYYVDVNKV 161          
BLAST of ES560392 vs. TrEMBL
Match: D7TIC6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033478001 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.940e-20
Identity = 48/64 (75.00%), Postives = 52/64 (81.25%), Query Frame = 1
Query:  151 MEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            M+ I D +QLDQIL  AQ   QPI+IDWMAAWCRKCIYLKPKLEKLAAE+DTK KFY VDV  V
Sbjct:    1 MQPIKDCDQLDQILARAQELPQPIIIDWMAAWCRKCIYLKPKLEKLAAEYDTKIKFYSVDVNNV 64          
BLAST of ES560392 vs. TrEMBL
Match: A6N1I7_ORYSI (Thioredoxin-like 5 OS=Oryza sativa subsp. indica PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.630e-17
Identity = 45/78 (57.69%), Postives = 58/78 (74.36%), Query Frame = 1
Query:  109 EAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            EA + D+S+P  VEME I+  E+LD  L  +  + QPI+IDWMA+WCRKCIYLKP+LEK+A EF    +FY+VDV KV
Sbjct:    6 EAYFWDVSKP--VEMEEIDSMEKLDDALRWSVENKQPIIIDWMASWCRKCIYLKPRLEKIAGEF-PGVRFYFVDVNKV 80          
BLAST of ES560392 vs. TrEMBL
Match: B6TXH7_MAIZE (Thioredoxin-like 5 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 8.436e-16
Identity = 42/80 (52.50%), Postives = 58/80 (72.50%), Query Frame = 1
Query:  103 RVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            R EA   D+SRP  VEME I+  E+LD  L  +  ++QP++IDWMA WCRKCIYL P++EK+A E+  + +FY++DV KV
Sbjct:   64 RPEAYCWDVSRP--VEMEEIDSIEKLDDALRWSVENNQPVVIDWMAGWCRKCIYLMPRMEKMAGEY-PEVRFYFIDVNKV 140          
BLAST of ES560392 vs. TrEMBL
Match: A9TX86_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_152180 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.102e-15
Identity = 40/71 (56.34%), Postives = 48/71 (67.61%), Query Frame = 1
Query:  121 PDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDV 333
            P + +P+ VEM AI   +Q D+ L        PI+IDWMA WCRKC+YLKPKLEKLAAE+    KFY VDV
Sbjct:    6 PSIPKPTWVEMTAITREDQFDEAL----ESGNPIIIDWMATWCRKCVYLKPKLEKLAAEYHPDVKFYVVDV 72          
BLAST of ES560392 vs. TAIR peptide
Match: AT5G06690.2 (| Symbols: WCRKC1 | WCRKC thioredoxin 1 | chr5:2060852-2061956 REVERSE LENGTH=214)

HSP 1 Score: 121.324 bits (303), Expect = 1.195e-28
Identity = 56/107 (52.34%), Postives = 79/107 (73.83%), Query Frame = 1
Query:   22 FCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            FC   Y   KL  S  +++       +R++A W ++++P+++EME IN+ E+LD +L HA+  SQPI+I+WMA+WCRKCIYLKPKLEKLAAE++ + KFYYVDV KV
Sbjct:   62 FCVSVYSNEKLGRSHMEKE-------WRLKAFWSNIAQPTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAKFYYVDVNKV 161          
BLAST of ES560392 vs. TAIR peptide
Match: AT5G06690.1 (| Symbols: WCRKC1 | WCRKC thioredoxin 1 | chr5:2060651-2061956 REVERSE LENGTH=210)

HSP 1 Score: 121.324 bits (303), Expect = 1.195e-28
Identity = 56/107 (52.34%), Postives = 79/107 (73.83%), Query Frame = 1
Query:   22 FCRKSYFGNKLYESRNKEQNKIKGRNFRVEAMWPDLSRPSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            FC   Y   KL  S  +++       +R++A W ++++P+++EME IN+ E+LD +L HA+  SQPI+I+WMA+WCRKCIYLKPKLEKLAAE++ + KFYYVDV KV
Sbjct:   62 FCVSVYSNEKLGRSHMEKE-------WRLKAFWSNIAQPTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAKFYYVDVNKV 161          
BLAST of ES560392 vs. TAIR peptide
Match: AT5G04260.1 (| Symbols: WCRKC2 | WCRKC thioredoxin 2 | chr5:1178901-1180022 REVERSE LENGTH=192)

HSP 1 Score: 82.0333 bits (201), Expect = 8.036e-17
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 1
Query:  136 PSSVEMEAINDSEQLDQILLHAQHDSQPILIDWMAAWCRKCIYLKPKLEKLAAEFDTKTKFYYVDVXKV 342
            P SVE+  I      DQ++  AQ   + ++I WMAAWCRKCIYLKPKLEKLAAEF  + +FY+VDV  V
Sbjct:   73 PVSVELGPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDVNAV 141          
The following BLAST results are available for this feature:
BLAST of ES560392 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
TRL31_ARATH1.503e-2752.34Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis... [more]
TRL31_ORYSJ9.768e-1957.50Thioredoxin-like 3-1, chloroplastic OS=Oryza sativ... [more]
TRL32_ARATH1.011e-1556.52Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis... [more]
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BLAST of ES560392 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FH98_MEDTR1.475e-4478.95Putative uncharacterized protein OS=Medicago trunc... [more]
C6TFV5_SOYBN2.604e-4174.34Putative uncharacterized protein OS=Glycine max PE... [more]
B9T802_RICCO1.692e-3272.04Electron transporter, putative OS=Ricinus communis... [more]
B9IIA5_POPTR7.108e-3159.29Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7LZT0_ARALY1.387e-2653.27Putative uncharacterized protein OS=Arabidopsis ly... [more]
Q2V396_ARATH3.090e-2652.34Putative uncharacterized protein At5g06690.2 OS=Ar... [more]
D7TIC6_VITVI1.940e-2075.00Whole genome shotgun sequence of line PN40024, sca... [more]
A6N1I7_ORYSI7.630e-1757.69Thioredoxin-like 5 OS=Oryza sativa subsp. indica P... [more]
B6TXH7_MAIZE8.436e-1652.50Thioredoxin-like 5 OS=Zea mays PE=2 SV=1[more]
A9TX86_PHYPA1.102e-1556.34Predicted protein OS=Physcomitrella patens subsp. ... [more]
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BLAST of ES560392 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT5G06690.21.195e-2852.34| Symbols: WCRKC1 | WCRKC thioredoxin 1 | chr5:206... [more]
AT5G06690.11.195e-2852.34| Symbols: WCRKC1 | WCRKC thioredoxin 1 | chr5:206... [more]
AT5G04260.18.036e-1756.52| Symbols: WCRKC2 | WCRKC thioredoxin 2 | chr5:117... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005746ThioredoxinPANTHERPTHR10438THIOREDOXIN-RELATEDcoord: 66..111
score: 3.3
IPR012335Thioredoxin foldGENE3D3.40.30.10no descriptioncoord: 49..113
score: 1.1
IPR012336Thioredoxin-like foldSUPERFAMILY52833Thioredoxin-likecoord: 48..113
score: 3.1
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 68..113
score: 1.3

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>ES560392 ID=ES560392; Name=ES560392; organism=Cicer arietinum; type=EST; length=342bp
GCGAGTGGAAGGGGTTATCTGTTTTGTCGCAAAAGTTATTTTGGGAACAA
ATTGTATGAAAGCAGAAACAAAGAACAAAACAAAATTAAAGGAAGGAATT
TTCGTGTGGAAGCTATGTGGCCAGATTTGTCAAGACCAAGTAGTGTAGAA
ATGGAAGCTATTAATGATTCTGAACAACTTGATCAAATTCTTCTTCATGC
TCAGCATGACTCTCAGCCTATCCTTATTGACTGGATGGCTGCTTGGTGCC
GGAAATGCATCTATTTGAAGCCAAAGTTGGAAAAATTAGCAGCTGAATTT
GACACCAAAACCAAATTTTATTATGTGGATGTCANTAAAGTT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0006662glycerol ether metabolic process
GO:0045454cell redox homeostasis
Vocabulary: Molecular Function
TermDefinition
GO:0009055electron transfer activity
GO:0015035protein-disulfide reductase activity
Vocabulary: INTERPRO
TermDefinition
IPR005746Thioredoxin
IPR012335Thioredoxin fold
IPR012336Thioredoxin-like_fold
IPR013766Thioredoxin_domain