FE668457

EST Overview


Name	FE668457
Unique Name	FE668457
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	571

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE668457 vs. SwissProt
Match: RA51D_ARATH (DNA repair protein RAD51 homolog 4 OS=Arabidopsis thaliana GN=RAD51D PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 3.692e-19
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  167 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 229

HSP 2 Score: 43.5134 bits (101), Expect = 3.692e-19
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ALM++ G+LLKKLAHEH+IA+L +  T  +
Sbjct:  229 GRALMVAIGYLLKKLAHEHSIAILVTNHTVGA 260

[Close]

BLAST of FE668457 vs. TrEMBL
Match: C6T870_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.143e-32
Identity = 58/71 (81.69%), Postives = 65/71 (91.55%), Query Frame = -1

Query:  359 NQADRRSLQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 571
            NQAD   L+KVLDRIICY VFDVY+MFD+LHQLKINL SEIVKS++HV+LLIVDSISSLITPIL G+GPQG
Sbjct:  167 NQADHM-LKKVLDRIICYSVFDVYQMFDVLHQLKINLRSEIVKSNQHVRLLIVDSISSLITPILGGSGPQG 236

HSP 2 Score: 51.2174 bits (121), Expect = 1.143e-32
Identity = 24/26 (92.31%), Postives = 25/26 (96.15%), Query Frame = -3

Query:  252 GHALMISAGFLLKKLAHEHNIAVLAS 329
            GHALMISAGFLLKKLAHEHNIAVL +
Sbjct:  236 GHALMISAGFLLKKLAHEHNIAVLVT 261

[Close]

BLAST of FE668457 vs. TrEMBL
Match: B9SYN5_RICCO (DNA repair and recombination protein radB, putative OS=Ricinus communis GN=RCOM_0787740 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.054e-20
Identity = 38/63 (60.32%), Postives = 47/63 (74.60%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            Q V+  I+C+ VFD++ MFD+LHQL+I+L S+    D  VQ LIVDSISSLITPIL G G QG
Sbjct:  168 QTVMSNILCHSVFDIFSMFDVLHQLEISLRSQGHSGDFKVQSLIVDSISSLITPILGGGGSQG 230

HSP 2 Score: 46.9802 bits (110), Expect = 2.054e-20
Identity = 22/26 (84.62%), Postives = 23/26 (88.46%), Query Frame = -3

Query:  252 GHALMISAGFLLKKLAHEHNIAVLAS 329
            GHALM SAG LLKKLAHEHNIAVL +
Sbjct:  230 GHALMTSAGILLKKLAHEHNIAVLVT 255

[Close]

BLAST of FE668457 vs. TrEMBL
Match: D7SKM2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025047001 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.776e-19
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = -1

Query:  359 LQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 550
            L+KV+ +I+C+ VFD++ M D++HQL+ +L S++      V+LLIVDSISSLITPIL G+G  G
Sbjct:  225 LEKVMSQILCHSVFDIFAMLDVVHQLEYSLRSQVQTGADQVRLLIVDSISSLITPILGGSGSHG 288

HSP 2 Score: 46.2098 bits (108), Expect = 2.776e-19
Identity = 21/26 (80.77%), Postives = 24/26 (92.31%), Query Frame = -3

Query:  252 GHALMISAGFLLKKLAHEHNIAVLAS 329
            G ALMISAGFLLKKLAHEHN+AV+ +
Sbjct:  288 GRALMISAGFLLKKLAHEHNLAVVVT 313

[Close]

BLAST of FE668457 vs. TrEMBL
Match: A5ARD8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_003133 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 3.613e-19
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = -1

Query:  359 LQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 550
            L+KV+ +I+C+ VFD++ M D++HQL+ +L S++      V+LLIVDSISSLITPIL G+G  G
Sbjct:  173 LEKVMSQILCHSVFDIFAMLDVVHQLEYSLRSQVQTGADQVRLLIVDSISSLITPILGGSGSHG 236

HSP 2 Score: 45.8246 bits (107), Expect = 3.613e-19
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = -3

Query:  258 GHALMISAGFLLKKLAHEHNIAVL 329
            G ALMISAGFLLKKLAHEHN+AV+
Sbjct:  236 GRALMISAGFLLKKLAHEHNLAVV 259

[Close]

BLAST of FE668457 vs. TrEMBL
Match: A3QS62_ARATH (RAD51D splice variant a OS=Arabidopsis thaliana GN=RAD51D PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.125e-18
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  167 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 229

HSP 2 Score: 43.5134 bits (101), Expect = 5.125e-18
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ALM++ G+LLKKLAHEH+IA+L +  T  +
Sbjct:  229 GRALMVAIGYLLKKLAHEHSIAILVTNHTVGA 260

[Close]

BLAST of FE668457 vs. TrEMBL
Match: A8MSF8_ARATH (Uncharacterized protein At1g07745.2 OS=Arabidopsis thaliana GN=At1g07745 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.134e-18
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  149 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 211

HSP 2 Score: 43.5134 bits (101), Expect = 5.134e-18
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ALM++ G+LLKKLAHEH+IA+L +  T  +
Sbjct:  211 GRALMVAIGYLLKKLAHEHSIAILVTNHTVGA 242

[Close]

BLAST of FE668457 vs. TrEMBL
Match: A3QS63_ARATH (RAD51D splice variant g OS=Arabidopsis thaliana GN=RAD51D PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.492e-17
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  167 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 229

HSP 2 Score: 41.2022 bits (95), Expect = 2.492e-17
Identity = 19/37 (51.35%), Postives = 27/37 (72.97%), Query Frame = -3

Query:  219 GHALMISAGFLLKKLAHEHNIAVLASGRTASSIRLFV 329
            G ALM++ G+LLKKLAHEH+IA+L   +  +  R+ V
Sbjct:  229 GRALMVAIGYLLKKLAHEHSIAILVLKKFQAIKRVLV 265

[Close]

BLAST of FE668457 vs. TrEMBL
Match: C4J8V1_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.632e-15
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = -1

Query:  359 RRSLQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSD-RHVQLLIVDSISSLITPILRGNGPQG 559
            +  L+ V+  IIC  VFD++ MF++LHQL+++L +E V  D R + LLI+DSISSL+ PI+ G  PQG
Sbjct:  147 KERLKTVMQSIICESVFDIFGMFEVLHQLEVSLLNEKVIGDGRRICLLIIDSISSLLAPIIGGKYPQG 214

HSP 2 Score: 37.3502 bits (85), Expect = 3.632e-15
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ++MIS   +LKKLA+EHN+AVL +    S+
Sbjct:  214 GRSMMISLAMILKKLAYEHNLAVLVTNHMVSA 245

[Close]

BLAST of FE668457 vs. TrEMBL
Match: B6TBD6_MAIZE (DNA repair protein RAD51 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.632e-15
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = -1

Query:  359 RRSLQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSD-RHVQLLIVDSISSLITPILRGNGPQG 559
            +  L+ V+  IIC  VFD++ MF++LHQL+++L +E V  D R + LLI+DSISSL+ PI+ G  PQG
Sbjct:  147 KERLKTVMQSIICESVFDIFGMFEVLHQLEVSLLNEKVIGDGRRICLLIIDSISSLLAPIIGGKYPQG 214

HSP 2 Score: 37.3502 bits (85), Expect = 3.632e-15
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ++MIS   +LKKLA+EHN+AVL +    S+
Sbjct:  214 GRSMMISLAMILKKLAYEHNLAVLVTNHMVSA 245

[Close]

BLAST of FE668457 vs. TrEMBL
Match: A2YYC5_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_30353 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.426e-13
Identity = 28/64 (43.75%), Postives = 47/64 (73.44%), Query Frame = -1

Query:  359 LQKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 550
            L++V+  IIC  VFD++++F++LHQL+++L S++      + LLI+DSISS++ PI  G  P+G
Sbjct:  142 LERVMSSIICKSVFDIFDLFEVLHQLELSLKSKVNNGGNKICLLIIDSISSILAPINGGKYPRG 205

HSP 2 Score: 35.039 bits (79), Expect = 1.426e-13
Identity = 14/26 (53.85%), Postives = 21/26 (80.77%), Query Frame = -3

Query:  252 GHALMISAGFLLKKLAHEHNIAVLAS 329
            G ++MIS   +LKKLA+EHN++VL +
Sbjct:  205 GRSMMISVAMILKKLAYEHNLSVLVT 230

[Close]

BLAST of FE668457 vs. TAIR peptide
Match: AT1G07745.1 (| Symbols: ATRAD51D, RAD51D, SSN1 | homolog of RAD51 D | chr1:2400797-2402842 REVERSE LENGTH=322)

HSP 1 Score: 71.633 bits (174), Expect = 3.914e-20
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  167 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 229

HSP 2 Score: 43.5134 bits (101), Expect = 3.914e-20
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ALM++ G+LLKKLAHEH+IA+L +  T  +
Sbjct:  229 GRALMVAIGYLLKKLAHEHSIAILVTNHTVGA 260

[Close]

BLAST of FE668457 vs. TAIR peptide
Match: AT1G07745.2 (| Symbols: ATRAD51D, RAD51D, SSN1 | homolog of RAD51 D | chr1:2400797-2402492 REVERSE LENGTH=304)

HSP 1 Score: 71.633 bits (174), Expect = 3.920e-20
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = -1

Query:  359 QKVLDRIICYPVFDVYEMFDMLHQLKINLTSEIVKSDRHVQLLIVDSISSLITPILRGNGPQG 547
            QKV+ RI+C+ V+D+Y +FD L  L+I L  ++  ++  ++LL+VDSISSLITPIL G+G QG
Sbjct:  149 QKVMSRILCHTVYDIYTLFDTLQDLEITLRLQMNVNESRLRLLVVDSISSLITPILGGSGSQG 211

HSP 2 Score: 43.5134 bits (101), Expect = 3.920e-20
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = -3

Query:  234 GHALMISAGFLLKKLAHEHNIAVLASGRTASS 329
            G ALM++ G+LLKKLAHEH+IA+L +  T  +
Sbjct:  211 GRALMVAIGYLLKKLAHEHSIAILVTNHTVGA 242

The following BLAST results are available for this feature:

BLAST of FE668457 vs. SwissProt
BLAST of FE668457 vs. TrEMBL
BLAST of FE668457 vs. TAIR peptide

BLAST of FE668457 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
RA51D_ARATH	3.692e-19	52.38	DNA repair protein RAD51 homolog 4 OS=Arabidopsis ...	[more]

BLAST of FE668457 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T870_SOYBN	1.143e-32	81.69	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SYN5_RICCO	2.054e-20	60.32	DNA repair and recombination protein radB, putativ...	[more]
D7SKM2_VITVI	2.776e-19	53.13	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5ARD8_VITVI	3.613e-19	53.13	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A3QS62_ARATH	5.125e-18	52.38	RAD51D splice variant a OS=Arabidopsis thaliana GN...	[more]
A8MSF8_ARATH	5.134e-18	52.38	Uncharacterized protein At1g07745.2 OS=Arabidopsis...	[more]
A3QS63_ARATH	2.492e-17	52.38	RAD51D splice variant g OS=Arabidopsis thaliana GN...	[more]
C4J8V1_MAIZE	3.632e-15	50.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TBD6_MAIZE	3.632e-15	50.00	DNA repair protein RAD51 OS=Zea mays PE=2 SV=1	[more]
A2YYC5_ORYSI	1.426e-13	43.75	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FE668457 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G07745.1	3.914e-20	52.38	\| Symbols: ATRAD51D, RAD51D, SSN1 \| homolog of RAD...	[more]
AT1G07745.2	3.920e-20	52.38	\| Symbols: ATRAD51D, RAD51D, SSN1 \| homolog of RAD...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 7..61 score: 0.0
None	No IPR available	PANTHER	PTHR22942	RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER	coord: 9..66 score: 1.8
None	No IPR available	PANTHER	PTHR22942:SF8	DNA REPAIR PROTEIN RAD51 HOMOLOG 4 (R51H4)	coord: 9..66 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE668457 ID=FE668457; Name=FE668457; organism=Cicer arietinum; type=EST; length=571bp
TGGATCCCCCGGGCTGCAGGTTTTTTTACAACATAAATGATCTTAATTAA
ATCATAGAAATTTTTGTTGCAAAAATATATTAGCACGTTTGTTCTTCTGG
TCCATATTTTCTGAAAACAACAGTACCGGTGCCAGTACCAGCATAAGCTT
CCACATACATTTCTATAAAATACAACATTCCTTTGATGCAACGCCATTTT
GCGGCGAGAATAGTTTCAAACAAACAGACGGATGGAAGATGCAGTCCTAC
CAGAAGCCAATACTGCAATATTATGCTCATGTGCTAACTTTTTCAGTAAA
AACCCAGCAGAAATCATTAAAGCGTGTCCAAGGAATTTTACATGCAAATA
TGTACTATACCCTGAGGACCATTGCCCCTAAGGATGGGAGTAATCAATGA
AGATATTGAATCAACAATAAGCAGCTGTACATGACGGTCTGATTTGACAA
TCTCAGATGTCAAATTGATCTTCAGTTGATGCAGCATATCAAACATCTCA
TATACATCAAACACCGGATAGCAAATTATCCTGTCCAATACTTTCTGTAG
AGATCTACGATCTGCCTGATT

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