FL518991

EST Overview


Name	FL518991
Unique Name	FL518991
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	412

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 3.367e-67
Identity = 117/136 (86.03%), Postives = 127/136 (93.38%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKNSIPDLANAGITHVWLPPPSQSV  +GYLPGRLYDLDASKYGSK+ELKSLI AF++KGIK LADIVINHRTAERKDGRGIYC+FEGGTPD++ DWGPSFIC+DDT YSDGTGN D+GE Y+AAPDIDHLNPQ
Sbjct:   42 NSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQ 177

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY3D_ORYSJ (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 4.126e-57
Identity = 96/136 (70.59%), Postives = 114/136 (83.82%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N+LK  + D+A AG+THVWLPPPS SV  QGY+PGRLYDLDASKYG+  ELKSLI AF+ KG++ +AD+VINHR AE+KD RG+YC+FEGGTPD +LDWGP  IC DDT YSDGTG+ DTGE + AAPDIDHLNP+
Sbjct:   45 NMLKGQVDDIAKAGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPR 180

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 217.624 bits (553), Expect = 1.568e-56
Identity = 98/134 (73.13%), Postives = 112/134 (83.58%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            N L   + D+A AGITHVWLPP SQSV  QGY+PGRLYDLDASKYG+K +LKSLI A + KG+K++ADIVINHRTAE KDGRGIYC+FEGGTPD +LDWGP  IC+DD  Y+DGTGN DTG D+ AAPDIDHLN
Sbjct:   44 NFLMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLN 177

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY1_ORYSJ (Alpha-amylase OS=Oryza sativa subsp. japonica GN=AMY1.1 PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 4.561e-56
Identity = 99/134 (73.88%), Postives = 114/134 (85.07%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            N L   + D+A AGITHVWLPPPS SVG QGY+PGRLYDLDASKYG++ +LKSLI+AF+ KG++ +ADIVINHRTAE KDGRGIYCLFEGGTPD++LDWGP  IC+DD  Y DGTGN DTG D+ AAPDIDHLN
Sbjct:   51 NFLMGKVDDIAAAGITHVWLPPPSHSVGEQGYMPGRLYDLDASKYGNEAQLKSLIEAFHGKGVQVIADIVINHRTAEHKDGRGIYCLFEGGTPDSRLDWGPHMICRDD-PYGDGTGNPDTGADFAAAPDIDHLN 183

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY3_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.3 PE=2 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 1.733e-55
Identity = 97/134 (72.39%), Postives = 111/134 (82.84%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            N L   + D+A AGITHVWLPP SQSV  QGY+PGRLYDLDASKYG+K +LKSLI A + KG+K++ADIVINHRTAE KDGRGIYC+FEG TPD +LDWGP  IC+DD  Y+DGTGN DTG D+ AAPDIDHLN
Sbjct:   44 NFLMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGVTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLN 177

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY2A_ORYSJ (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica GN=AMY2A PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 6.587e-55
Identity = 94/134 (70.15%), Postives = 112/134 (83.58%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            NLL   + D+  AG+THVWLPPPS SV +QGY+PGRLYDLDAS+YG+  ELKSLI A + KGI+++AD+VINHR A+ KD RGIYC+FEGGTPD +LDWGP  IC+DDT +SDGTGNLDTG D+ AAPDIDHLN
Sbjct:   42 NLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPGRLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDGRLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLN 175

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY2A_ORYSI (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 6.587e-55
Identity = 94/134 (70.15%), Postives = 112/134 (83.58%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            NLL   + D+  AG+THVWLPPPS SV +QGY+PGRLYDLDAS+YG+  ELKSLI A + KGI+++AD+VINHR A+ KD RGIYC+FEGGTPD +LDWGP  IC+DDT +SDGTGNLDTG D+ AAPDIDHLN
Sbjct:   42 NLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPGRLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDGRLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLN 175

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 1.916e-54
Identity = 95/135 (70.37%), Postives = 114/135 (84.44%), Query Frame = -3

Query:    6 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNP 410
            N+LK+ + D+A+AG+THVWLPPP+ SV  QGY+PGRLYDL+ASKYG+K ELKSLI AF+ KGIK +ADIV+NHR A+ KDGRG+YC+F+GG P   LDWGPS IC DDT YSDGTG+ DTG D+ AAPDIDHLNP
Sbjct:   48 NMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLNASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGPSMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNP 182

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY6_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 208.764 bits (530), Expect = 7.282e-54
Identity = 96/138 (69.57%), Postives = 111/138 (80.43%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGN--LDTGEDYNAAPDIDHLNPQ 410
            N L   + D+A AG+THVWLPP SQSV  QGY+PGRLYDLDASKYG+K +LKSLI A + K +K++ADIVINHRTAERKDGRGIYC+FEGGTPD +LDWGP  IC+DD  Y DGTGN    T  D+ AAPDIDHLNP+
Sbjct:   44 NFLMGKVDDIAAAGVTHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKAVKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYPDGTGNRPTRTRADFGAAPDIDHLNPR 181

[Close]

BLAST of FL518991 vs. SwissProt
Match: AMY3B_ORYSJ (Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica GN=AMY1.6 PE=2 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 2.767e-53
Identity = 93/134 (69.40%), Postives = 108/134 (80.60%), Query Frame = -3

Query:    9 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLN 410
            N L   + D+A  G+THVWLPPPS SV  QGY+PGRLYDLDASKYG+  EL+SLI AF+ KGIK +ADIVINHR A+ KD RGIYC+FEGGTPD++LDWGP  IC DDT YS+G G+ DTG D+ AAPDIDHLN
Sbjct:   46 NFLHGHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTGAELRSLIAAFHSKGIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLN 179

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q9ZP43_PHAVU (Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 2.126e-67
Identity = 118/136 (86.76%), Postives = 128/136 (94.12%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKNS+PD+ANAGITHVWLPPPSQSV  +GYLPGRLYDLDAS+YGSKDELKSLI AF++KGIK LADIVINHRTAERKDGRGIYC+FEGGTPD +LDWGPSFICKDDTTYSDGTGN D+GE Y+AAPDIDHLNPQ
Sbjct:   42 NSLKNSVPDIANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASRYGSKDELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPSFICKDDTTYSDGTGNNDSGESYDAAPDIDHLNPQ 177

[Close]

BLAST of FL518991 vs. TrEMBL
Match: C6T9Z1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 4.736e-67
Identity = 118/136 (86.76%), Postives = 127/136 (93.38%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKN+IPDLANAGITHVWLPPPSQSV  +GYLPGRLYDLDASKYG+KD+LKSLI AF+DKGIK LADIVINHRTAERKDGRGIYC+FEGGTPD +LDWGPSFICKDD TYSDGTGNLD+GE Y+ APDIDHLNPQ
Sbjct:   42 NSLKNTIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYGTKDQLKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPSFICKDDNTYSDGTGNLDSGEPYDPAPDIDHLNPQ 177

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q7X9T1_PHAAN (Alpha-amylase OS=Phaseolus angularis PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 6.839e-66
Identity = 117/136 (86.03%), Postives = 127/136 (93.38%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKNSIPDLANAGITHVWLPPPSQSV  +GYLPGRLYDLDAS+YGSK+ELKSLI AF +KGIK LADIVINHRTAERKDGRGIYC+FEGGTPD++LDWGPSFIC+DDT YSDGTGN D+GE Y+AAPDIDHLNPQ
Sbjct:   42 NSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASRYGSKNELKSLIAAFYEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRLDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQ 177

[Close]

BLAST of FL518991 vs. TrEMBL
Match: B2KIQ7_MANES (Alpha-amylase (Fragment) OS=Manihot esculenta PE=4 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 2.277e-61
Identity = 109/136 (80.15%), Postives = 120/136 (88.24%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKN IPDLANAG+THVWLPPPSQS   QGYLPGRLYDL+ASKYG++DEL SLI +F+ KGIKSLADIVINHRTAE+KD RGIYC+FEGGTPD  LDWGPSFIC+DDT YSDG GN DTGED+  APDIDHLNP+
Sbjct:   16 NSLKNMIPDLANAGVTHVWLPPPSQSAAPQGYLPGRLYDLNASKYGTQDELVSLIDSFHQKGIKSLADIVINHRTAEKKDDRGIYCIFEGGTPDGTLDWGPSFICRDDTAYSDGQGNPDTGEDFKGAPDIDHLNPR 151

[Close]

BLAST of FL518991 vs. TrEMBL
Match: B9R7C6_RICCO (Pentatricopeptide repeat-containing protein, putative OS=Ricinus communis GN=RCOM_1590660 PE=3 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 1.130e-60
Identity = 109/136 (80.15%), Postives = 123/136 (90.44%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            NLLKNSI D+ANAGITHVWLPPPSQSV  QGY+PGRLYDL+AS+YG++DELKSLI+AF+ KGIK LADIVINHRTAE+KD RGI+C+FEGGT D +LDWGPSFIC DDT +SDGTGN DTGEDY  APDIDHLNP+
Sbjct:   45 NLLKNSILDIANAGITHVWLPPPSQSVSPQGYMPGRLYDLNASRYGTQDELKSLIRAFHHKGIKCLADIVINHRTAEKKDERGIWCIFEGGTDDDRLDWGPSFICCDDTVFSDGTGNPDTGEDYPPAPDIDHLNPR 180

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q8LP27_IPONI (Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.381e-58
Identity = 103/136 (75.74%), Postives = 117/136 (86.03%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N L NSIPDLANAGITHVWLPPPS SV  QGY+PGRLYDLDASKYG+K +L++L+ A +DKGIK++ADIVINHR A+ KD RGIYC+FEGGTPD +LDWGP  ICKDDT YSDGTGN DTG D+  APDIDHLNP+
Sbjct:   42 NSLINSIPDLANAGITHVWLPPPSHSVAPQGYMPGRLYDLDASKYGNKQQLQALVAALHDKGIKAVADIVINHRCADYKDSRGIYCIFEGGTPDDRLDWGPGLICKDDTQYSDGTGNADTGMDFGGAPDIDHLNPR 177

[Close]

BLAST of FL518991 vs. TrEMBL
Match: B9GNW3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_644125 PE=3 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 5.248e-58
Identity = 105/136 (77.21%), Postives = 116/136 (85.29%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LKN +PDLANAGITHVWLPP SQSV  QGY+PGRLYDL ASKYGS+ ELKSLI AF+ KGIK LADIVINHRTAE++DGRGI+C+FEGGT D +LDWGPS IC DDT YSDG GN DTG D+  APDIDHLNP+
Sbjct:   23 NSLKNLVPDLANAGITHVWLPPASQSVAPQGYMPGRLYDLSASKYGSQGELKSLIGAFHQKGIKCLADIVINHRTAEKQDGRGIWCIFEGGTSDDRLDWGPSLICSDDTAYSDGKGNPDTGGDFGGAPDIDHLNPR 158

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q33DV1_HYAOR (Alpha-amylase OS=Hyacinthus orientalis GN=HoAmy1A PE=2 SV=2)

HSP 1 Score: 226.098 bits (575), Expect = 8.952e-58
Identity = 101/136 (74.26%), Postives = 116/136 (85.29%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LK  + D++++G THVWLPPPS SVG QGYLPGRLYDL ASKYG++DELKSLI+AF+DK IK +ADIVINHRTAE KDGRGIYC+FEGGTPD +LDWGP+ IC+DD  YSDGTGN DTG  Y AAPDIDH NP+
Sbjct:   46 NFLKTKVDDISSSGATHVWLPPPSHSVGQQGYLPGRLYDLSASKYGNQDELKSLIQAFHDKSIKCVADIVINHRTAETKDGRGIYCIFEGGTPDDRLDWGPTMICRDDAEYSDGTGNPDTGAGYGAAPDIDHTNPR 181

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q8LJQ6_MUSAC (Alpha-amylase OS=Musa acuminata PE=2 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.169e-57
Identity = 100/136 (73.53%), Postives = 119/136 (87.50%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LK+ + D+ANAG+THVWLPPPS SVG QGY+PGRLYDL ASKYG++DELK+LI AF+DKG+K +ADIVINHR A+++DGRGI+C+FEGGT D +LDWGP  IC+DDT YSDGTGNLDTGE + AAPDIDHLN Q
Sbjct:   35 NFLKDKVSDIANAGVTHVWLPPPSHSVGVQGYMPGRLYDLGASKYGNQDELKALIGAFHDKGVKCVADIVINHRCADKQDGRGIWCIFEGGTDDARLDWGPHMICRDDTQYSDGTGNLDTGEGFAAAPDIDHLNTQ 170

[Close]

BLAST of FL518991 vs. TrEMBL
Match: Q8GUR0_MUSAC (Alpha-amylase OS=Musa acuminata PE=3 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.169e-57
Identity = 100/136 (73.53%), Postives = 119/136 (87.50%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N LK+ + D+ANAG+THVWLPPPS SVG QGY+PGRLYDL ASKYG++DELK+LI AF+DKG+K +ADIVINHR A+++DGRGI+C+FEGGT D +LDWGP  IC+DDT YSDGTGNLDTGE + AAPDIDHLN Q
Sbjct:   35 NFLKDKVSDIANAGVTHVWLPPPSHSVGVQGYMPGRLYDLGASKYGNQDELKALIGAFHDKGVKCVADIVINHRCADKQDGRGIWCIFEGGTDDARLDWGPHMICRDDTQYSDGTGNLDTGEGFAAAPDIDHLNTQ 170

[Close]

BLAST of FL518991 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 209.149 bits (531), Expect = 6.329e-55
Identity = 98/136 (72.06%), Postives = 115/136 (84.56%), Query Frame = -3

Query:    3 NLLKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDHLNPQ 410
            N L NSI D+ANAGITH+WLPPPSQSV  +GYLPG+LYDL++SKYGS+ ELKSLIKA N KGIK+LADIVINHRTAERKD +  YC FEGGT D +LDW PSF+C++D  +  GTGNLDTG D++ APDIDHLNP+
Sbjct:   45 NSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPR 179

[Close]

BLAST of FL518991 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 110.153 bits (274), Expect = 4.001e-25
Identity = 56/130 (43.08%), Postives = 84/130 (64.62%), Query Frame = -3

Query:   15 LKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDH 404
            L+    +LA+ G T +WLPPP++SV  +GY+P  LY+L+ S+YG+ DELK  +K F+  GIK L D V+NHR A  K+  G++ LF G     +L+W    +  DD  +  G GN  +G++++AAP+IDH
Sbjct:  516 LQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDH 638

[Close]

BLAST of FL518991 vs. TAIR peptide
Match: AT1G76130.1 (| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr1:28561647-28563914 FORWARD LENGTH=413)

HSP 1 Score: 103.219 bits (256), Expect = 4.891e-23
Identity = 52/130 (40.00%), Postives = 77/130 (59.23%), Query Frame = -3

Query:   15 LKNSIPDLANAGITHVWLPPPSQSVGSQGYLPGRLYDLDASKYGSKDELKSLIKAFNDKGIKSLADIVINHRTAERKDGRGIYCLFEGGTPDTKLDWGPSFICKDDTTYSDGTGNLDTGEDYNAAPDIDH 404
            L   +PD+A +G T  WLPPPSQS+  +GYLP  LY L+ S YGS+  LKSL++      ++++ADIVINHR    +   G+Y  ++G      L W    +    T+ + G GN  TG+++N  P++DH
Sbjct:   45 LDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDH 165

The following BLAST results are available for this feature:

BLAST of FL518991 vs. SwissProt
BLAST of FL518991 vs. TrEMBL
BLAST of FL518991 vs. TAIR peptide

BLAST of FL518991 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AMYA_VIGMU	3.367e-67	86.03	Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1	[more]
AMY3D_ORYSJ	4.126e-57	70.59	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. ja...	[more]
AMY2_HORVU	1.568e-56	73.13	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY1_ORYSJ	4.561e-56	73.88	Alpha-amylase OS=Oryza sativa subsp. japonica GN=A...	[more]
AMY3_HORVU	1.733e-55	72.39	Alpha-amylase type B isozyme (Fragment) OS=Hordeum...	[more]
AMY2A_ORYSJ	6.587e-55	70.15	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. ja...	[more]
AMY2A_ORYSI	6.587e-55	70.15	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. in...	[more]
AMY3A_ORYSJ	1.916e-54	70.37	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. ja...	[more]
AMY6_HORVU	7.282e-54	69.57	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY3B_ORYSJ	2.767e-53	69.40	Alpha-amylase isozyme 3B OS=Oryza sativa subsp. ja...	[more]

BLAST of FL518991 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9ZP43_PHAVU	2.126e-67	86.76	Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1	[more]
C6T9Z1_SOYBN	4.736e-67	86.76	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q7X9T1_PHAAN	6.839e-66	86.03	Alpha-amylase OS=Phaseolus angularis PE=2 SV=1	[more]
B2KIQ7_MANES	2.277e-61	80.15	Alpha-amylase (Fragment) OS=Manihot esculenta PE=4...	[more]
B9R7C6_RICCO	1.130e-60	80.15	Pentatricopeptide repeat-containing protein, putat...	[more]
Q8LP27_IPONI	1.381e-58	75.74	Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1	[more]
B9GNW3_POPTR	5.248e-58	77.21	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q33DV1_HYAOR	8.952e-58	74.26	Alpha-amylase OS=Hyacinthus orientalis GN=HoAmy1A ...	[more]
Q8LJQ6_MUSAC	1.169e-57	73.53	Alpha-amylase OS=Musa acuminata PE=2 SV=1	[more]
Q8GUR0_MUSAC	1.169e-57	73.53	Alpha-amylase OS=Musa acuminata PE=3 SV=1	[more]

BLAST of FL518991 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G25000.1	6.329e-55	72.06	\| Symbols: ATAMY1, AMY1 \| alpha-amylase-like \| chr...	[more]
AT1G69830.1	4.001e-25	43.08	\| Symbols: ATAMY3, AMY3 \| alpha-amylase-like 3 \| c...	[more]
AT1G76130.1	4.891e-23	40.00	\| Symbols: ATAMY2, AMY2 \| alpha-amylase-like 2 \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 5..81 score: 2.7
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 4..137 score: 1.4
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..137 score: 1.6
None	No IPR available	PANTHER	PTHR10357	AMYLASE	coord: 3..137 score: 2.2
None	No IPR available	PANTHER	PTHR10357:SF9	ALPHA-AMYLASE	coord: 3..137 score: 2.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL518991 ID=FL518991; Name=FL518991; organism=Cicer arietinum; type=EST; length=412bp
ACTTGAGGATTTAAATGATCAATGTCAGGTGCAGCATTGTAATCTTCTCC
AGTATCAAGATTTCCAGTGCCGTCTGAATAAGTTGTGTCATCTTTGCAAA
TGAAAGATGGACCCCAATCAAGTTTTGTATCTGGGGTCCCACCTTCAAAG
AGACAGTAAATACCTCTTCCATCTTTTCTCTCTGCAGTTCTATGGTTGAT
TACTATGTCAGCTAGGCTCTTTATTCCTTTATCATTAAAGGCCTTTATAA
GGGACTTTAGTTCATCCTTGGAACCATATTTTGATGCATCAAGATCATAT
AGTCTTCCTGGAAGATACCCTTGAGAACCAACACTTTGAGATGGAGGAGG
GAGCCAAACATGAGTAATTCCTGCATTTGCTAAGTCAGGAATGGAGTTCT
TCAACAAGTTGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR006047	Glyco_hydro_13_cat_dom
IPR013781	Glyco_hydro_catalytic_dom
IPR017853	Glycoside_hydrolase_SF

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