GR398556

EST Overview


Name	GR398556
Unique Name	GR398556
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	430

Libraries


Library Name	Type
LIBEST_024782 ICC4958 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR398556 vs. SwissProt
Match: SCP47_ARATH (Serine carboxypeptidase-like 47 OS=Arabidopsis thaliana GN=SCPL47 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.414e-17
Identity = 39/52 (75.00%), Postives = 46/52 (88.46%), Query Frame = -1

Query:  275 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKT 430
            FLVDG+EAG+L++HGPL F KV  AGHMVPMDQPKA+LQML++WMQGKL  T
Sbjct:  447 FLVDGKEAGLLKNHGPLTFLKVYNAGHMVPMDQPKASLQMLQNWMQGKLRTT 498

[Close]

BLAST of GR398556 vs. SwissProt
Match: CBPX_PEA (Serine carboxypeptidase-like (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.071e-14
Identity = 33/56 (58.93%), Postives = 47/56 (83.93%), Query Frame = -1

Query:  263 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGD 430
            F+V+G +AG+L+S+GPL+F KV +AGHMVPMDQPKAAL+M+K W +G L ++  G+
Sbjct:  224 FVVNGSQAGLLKSYGPLSFLKVHDAGHMVPMDQPKAALEMVKQWTRGTLAESIDGE 279

[Close]

BLAST of GR398556 vs. SwissProt
Match: SCP48_ARATH (Serine carboxypeptidase-like 48 OS=Arabidopsis thaliana GN=SCPL48 PE=2 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.705e-14
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  281 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLT 430
            F VD +EAG+++++G L F KV +AGHMVPMDQPKAALQML++WMQGKL+
Sbjct:  452 FHVDNKEAGLMKNYGSLTFLKVHDAGHMVPMDQPKAALQMLQNWMQGKLS 501

[Close]

BLAST of GR398556 vs. SwissProt
Match: CBP3_ORYSJ (Serine carboxypeptidase 3 OS=Oryza sativa subsp. japonica GN=CBP3 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.705e-14
Identity = 35/49 (71.43%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  284 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKL 430
            F+VDG EAG+L+SHGPL+F KV  AGHMVPMDQPKA+L+ML+ + QGKL
Sbjct:  435 FVVDGAEAGVLKSHGPLSFLKVHNAGHMVPMDQPKASLEMLRRFTQGKL 483

[Close]

BLAST of GR398556 vs. SwissProt
Match: CBPX_ORYSJ (Serine carboxypeptidase-like OS=Oryza sativa subsp. japonica GN=CBP31 PE=2 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 1.754e-13
Identity = 33/50 (66.00%), Postives = 41/50 (82.00%), Query Frame = -1

Query:  281 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLT 430
            F VDG+EAG+L+S+GPL+F KV +AGHMVPMDQPK AL+ML  W  G L+
Sbjct:  367 FTVDGKEAGILKSYGPLSFLKVHDAGHMVPMDQPKVALEMLMRWTSGNLS 416

[Close]

BLAST of GR398556 vs. SwissProt
Match: SCP49_ARATH (Serine carboxypeptidase-like 49 OS=Arabidopsis thaliana GN=SCPL49 PE=2 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 2.991e-13
Identity = 32/49 (65.31%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  284 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKL 430
            F+VDG+EAG+L+++  L+F KV++AGHMVPMDQPKAAL+MLK WM+  L
Sbjct:  448 FIVDGKEAGLLKTYEQLSFLKVRDAGHMVPMDQPKAALKMLKRWMENSL 496

[Close]

BLAST of GR398556 vs. SwissProt
Match: CBP3_WHEAT (Serine carboxypeptidase 3 OS=Triticum aestivum GN=CBP3 PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.102e-13
Identity = 34/52 (65.38%), Postives = 43/52 (82.69%), Query Frame = -1

Query:  275 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKT 430
            FLVD  +AG+L+SHG L+F KV  AGHMVPMDQPKAAL+ML+ + QGKL ++
Sbjct:  435 FLVDDAQAGVLKSHGALSFLKVHNAGHMVPMDQPKAALEMLRRFTQGKLKES 486

[Close]

BLAST of GR398556 vs. SwissProt
Match: CBP3_HORVU (Serine carboxypeptidase 3 OS=Hordeum vulgare GN=CBP3 PE=1 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 6.664e-13
Identity = 34/49 (69.39%), Postives = 41/49 (83.67%), Query Frame = -1

Query:  284 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKL 430
            FLVD  +AG+L+SHG L+F KV  AGHMVPMDQPKAAL+ML+ + QGKL
Sbjct:  442 FLVDDAQAGVLKSHGALSFLKVHNAGHMVPMDQPKAALEMLRRFTQGKL 490

[Close]

BLAST of GR398556 vs. SwissProt
Match: YBY9_YEAST (Putative serine carboxypeptidase YBR139W OS=Saccharomyces cerevisiae GN=YBR139W PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.897e-8
Identity = 21/43 (48.84%), Postives = 33/43 (76.74%), Query Frame = -1

Query:  290 GEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQG 418
            GEE G ++++GP  F ++ +AGHMVP DQP+A+L+M+  W+ G
Sbjct:  452 GEELGQVKNYGPFTFLRIYDAGHMVPYDQPEASLEMVNSWISG 494

[Close]

BLAST of GR398556 vs. TrEMBL
Match: Q41691_9FABA (Serine carboxypeptidase (Fragment) OS=Vigna radiata PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.310e-18
Identity = 44/56 (78.57%), Postives = 47/56 (83.93%), Query Frame = -1

Query:  263 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGD 430
            FLVDG EAG L+SHGPLAF KV EAGHMVPMDQPKAAL  L+ WMQGKLT T+ GD
Sbjct:  235 FLVDGAEAGTLKSHGPLAFLKVYEAGHMVPMDQPKAALTTLRSWMQGKLTMTKNGD 290

[Close]

BLAST of GR398556 vs. TrEMBL
Match: B9N866_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1117480 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.495e-17
Identity = 42/60 (70.00%), Postives = 48/60 (80.00%), Query Frame = -1

Query:  251 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGDSVSP 430
            F+V+G EAG L+SHGPL+F KV  AGHMVPMDQPKAALQMLK WMQGKL  T   D ++P
Sbjct:  441 FVVEGREAGQLKSHGPLSFLKVHNAGHMVPMDQPKAALQMLKSWMQGKLAVTGTKDWIAP 500

[Close]

BLAST of GR398556 vs. TrEMBL
Match: B9SCI8_RICCO (Serine carboxypeptidase, putative OS=Ricinus communis GN=RCOM_0473920 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.970e-16
Identity = 42/60 (70.00%), Postives = 47/60 (78.33%), Query Frame = -1

Query:  251 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGDSVSP 430
            F V+G EAG L+SHGPL F KV EAGHMVPMDQPKAALQML  WMQGKL  T + + V+P
Sbjct:  447 FKVEGAEAGQLKSHGPLTFLKVNEAGHMVPMDQPKAALQMLTSWMQGKLADTNR-EKVTP 505

[Close]

BLAST of GR398556 vs. TrEMBL
Match: D7SNC4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_61.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031490001 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.446e-15
Identity = 40/56 (71.43%), Postives = 44/56 (78.57%), Query Frame = -1

Query:  263 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGD 430
            +LVDG+EAG L++HGPLAF KV  AGHMVPMDQPKAALQMLK W QGKL      D
Sbjct:  404 YLVDGKEAGQLKNHGPLAFLKVHNAGHMVPMDQPKAALQMLKTWTQGKLAPIETKD 459

[Close]

BLAST of GR398556 vs. TrEMBL
Match: Q9XH61_9ASTR (Serine carboxypeptidase OS=Matricaria chamomilla PE=2 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 3.221e-15
Identity = 38/55 (69.09%), Postives = 47/55 (85.45%), Query Frame = -1

Query:  266 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKG 430
            F VDG EAG+L+S+GPL+F KV +AGHMVPMDQPKAAL+MLK WM G L++T +G
Sbjct:  439 FEVDGSEAGLLKSYGPLSFLKVHDAGHMVPMDQPKAALEMLKRWMDGSLSETPRG 493

[Close]

BLAST of GR398556 vs. TrEMBL
Match: D7M1L4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489152 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.495e-15
Identity = 37/50 (74.00%), Postives = 46/50 (92.00%), Query Frame = -1

Query:  281 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLT 430
            FLVDG+EAG L+++GPL F KV +AGHMVPMDQPKA+LQML++WMQGKL+
Sbjct:  450 FLVDGKEAGSLKNYGPLTFLKVHDAGHMVPMDQPKASLQMLQNWMQGKLS 499

[Close]

BLAST of GR398556 vs. TrEMBL
Match: B9SUC4_RICCO (Serine carboxypeptidase, putative OS=Ricinus communis GN=RCOM_0721950 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.224e-14
Identity = 41/63 (65.08%), Postives = 50/63 (79.37%), Query Frame = -1

Query:  248 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTK--TRKGDSVSPM 430
            F VD  EAG+L+S+GPLAF KV +AGHMVPMDQPKAAL+MLK W QGKL++  T+ G  V+ M
Sbjct:  447 FTVDNSEAGVLRSYGPLAFLKVHDAGHMVPMDQPKAALEMLKRWTQGKLSEALTQPGKLVAEM 509

[Close]

BLAST of GR398556 vs. TrEMBL
Match: D7SNC3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_61.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031488001 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.727e-14
Identity = 39/57 (68.42%), Postives = 44/57 (77.19%), Query Frame = -1

Query:  260 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKTRKGDS 430
            +LVDG+EAG L+ HG LAF KV  AGHMVPMDQPKAALQMLK W QGKL    K ++
Sbjct:  443 YLVDGKEAGQLKYHGRLAFLKVHNAGHMVPMDQPKAALQMLKTWTQGKLAPKIKDET 499

[Close]

BLAST of GR398556 vs. TrEMBL
Match: C5X8I6_SORBI (Putative uncharacterized protein Sb02g033170 OS=Sorghum bicolor GN=Sb02g033170 PE=4 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.562e-14
Identity = 36/51 (70.59%), Postives = 44/51 (86.27%), Query Frame = -1

Query:  278 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTK 430
            F VDG+EAG+L+SHGPL+F KV +AGHMVPMDQPKAAL+MLK W  G L++
Sbjct:  459 FTVDGKEAGVLKSHGPLSFLKVHDAGHMVPMDQPKAALEMLKRWTSGNLSE 509

[Close]

BLAST of GR398556 vs. TrEMBL
Match: B4FU35_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.562e-14
Identity = 36/51 (70.59%), Postives = 44/51 (86.27%), Query Frame = -1

Query:  278 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTK 430
            F VDG+EAG+L+SHGPL+F KV +AGHMVPMDQPKAAL+MLK W  G L++
Sbjct:  335 FTVDGKEAGVLKSHGPLSFLKVHDAGHMVPMDQPKAALEMLKRWTSGNLSE 385

[Close]

BLAST of GR398556 vs. TAIR peptide
Match: AT5G22980.1 (| Symbols: scpl47 | serine carboxypeptidase-like 47 | chr5:7688084-7690481 FORWARD LENGTH=505)

HSP 1 Score: 87.0409 bits (214), Expect = 4.179e-18
Identity = 39/52 (75.00%), Postives = 46/52 (88.46%), Query Frame = -1

Query:  275 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLTKT 430
            FLVDG+EAG+L++HGPL F KV  AGHMVPMDQPKA+LQML++WMQGKL  T
Sbjct:  447 FLVDGKEAGLLKNHGPLTFLKVYNAGHMVPMDQPKASLQMLQNWMQGKLRTT 498

[Close]

BLAST of GR398556 vs. TAIR peptide
Match: AT3G45010.1 (| Symbols: scpl48 | serine carboxypeptidase-like 48 | chr3:16466328-16468845 FORWARD LENGTH=510)

HSP 1 Score: 77.411 bits (189), Expect = 3.311e-15
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  281 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKLT 430
            F VD +EAG+++++G L F KV +AGHMVPMDQPKAALQML++WMQGKL+
Sbjct:  452 FHVDNKEAGLMKNYGSLTFLKVHDAGHMVPMDQPKAALQMLQNWMQGKLS 501

[Close]

BLAST of GR398556 vs. TAIR peptide
Match: AT3G10410.1 (| Symbols: SCPL49, CPY | SERINE CARBOXYPEPTIDASE-LIKE 49 | chr3:3235518-3238063 REVERSE LENGTH=516)

HSP 1 Score: 73.9442 bits (180), Expect = 3.661e-14
Identity = 32/49 (65.31%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  284 FLVDGEEAGMLQSHGPLAFFKVKEAGHMVPMDQPKAALQMLKDWMQGKL 430
            F+VDG+EAG+L+++  L+F KV++AGHMVPMDQPKAAL+MLK WM+  L
Sbjct:  448 FIVDGKEAGLLKTYEQLSFLKVRDAGHMVPMDQPKAALKMLKRWMENSL 496

The following BLAST results are available for this feature:

BLAST of GR398556 vs. SwissProt
BLAST of GR398556 vs. TrEMBL
BLAST of GR398556 vs. TAIR peptide

BLAST of GR398556 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
SCP47_ARATH	3.414e-17	75.00	Serine carboxypeptidase-like 47 OS=Arabidopsis tha...	[more]
CBPX_PEA	2.071e-14	58.93	Serine carboxypeptidase-like (Fragment) OS=Pisum s...	[more]
SCP48_ARATH	2.705e-14	68.00	Serine carboxypeptidase-like 48 OS=Arabidopsis tha...	[more]
CBP3_ORYSJ	2.705e-14	71.43	Serine carboxypeptidase 3 OS=Oryza sativa subsp. j...	[more]
CBPX_ORYSJ	1.754e-13	66.00	Serine carboxypeptidase-like OS=Oryza sativa subsp...	[more]
SCP49_ARATH	2.991e-13	65.31	Serine carboxypeptidase-like 49 OS=Arabidopsis tha...	[more]
CBP3_WHEAT	5.102e-13	65.38	Serine carboxypeptidase 3 OS=Triticum aestivum GN=...	[more]
CBP3_HORVU	6.664e-13	69.39	Serine carboxypeptidase 3 OS=Hordeum vulgare GN=CB...	[more]
YBY9_YEAST	2.897e-8	48.84	Putative serine carboxypeptidase YBR139W OS=Saccha...	[more]

BLAST of GR398556 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q41691_9FABA	5.310e-18	78.57	Serine carboxypeptidase (Fragment) OS=Vigna radiat...	[more]
B9N866_POPTR	4.495e-17	70.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SCI8_RICCO	4.970e-16	70.00	Serine carboxypeptidase, putative OS=Ricinus commu...	[more]
D7SNC4_VITVI	1.446e-15	71.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9XH61_9ASTR	3.221e-15	69.09	Serine carboxypeptidase OS=Matricaria chamomilla P...	[more]
D7M1L4_ARALY	5.495e-15	74.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9SUC4_RICCO	1.224e-14	65.08	Serine carboxypeptidase, putative OS=Ricinus commu...	[more]
D7SNC3_VITVI	2.727e-14	68.42	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5X8I6_SORBI	3.562e-14	70.59	Putative uncharacterized protein Sb02g033170 OS=So...	[more]
B4FU35_MAIZE	3.562e-14	70.59	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of GR398556 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G22980.1	4.179e-18	75.00	\| Symbols: scpl47 \| serine carboxypeptidase-like 4...	[more]
AT3G45010.1	3.311e-15	68.00	\| Symbols: scpl48 \| serine carboxypeptidase-like 4...	[more]
AT3G10410.1	3.661e-14	65.31	\| Symbols: SCPL49, CPY \| SERINE CARBOXYPEPTIDASE-L...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001563	Peptidase S10, serine carboxypeptidase	PANTHER	PTHR11802	SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE	coord: 1..48 score: 3.6
IPR001563	Peptidase S10, serine carboxypeptidase	PFAM	PF00450	Peptidase_S10	coord: 4..47 score: 1.8
None	No IPR available	GENE3D	3.40.50.1820	no description	coord: 3..52 score: 2.9
None	No IPR available	PANTHER	PTHR11802:SF6	SERINE CARBOXYPEPTIDASE CPVL/CARBOXYPEPTIDASE Y(YEAST)	coord: 1..48 score: 3.6
None	No IPR available	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 1..50 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR398556 ID=GR398556; Name=GR398556; organism=Cicer arietinum; type=EST; length=430bp
CCAAAATGCAATCTTGTTTTAAAAAAATCAAATACAAAGTTTTGCATGAT
TTGATAACAATAATGGATGACTTATTTCAAATCCATCAATTCACAATTTA
TGAAATAAGTAAAAGCAGGAATGCTAAAAATTAAAAAAAAACTTGGGGAA
AAACAATTATATTCCATTCATCCACTATTCCAATGTACAAAAGCAAACAT
CATTATTGTGTGTACTACAATAGTGTGATTTTGTGTTGACTGCATCACAT
AGGCGAAACACTATCTCCTTTCCTAGTCTTTGTTAGTTTCCCTTGCATCC
AATCTTTTAACATCTGAAGTGCAGCTTTTGGTTGATCCATAGGAACCATG
TGACCAGCCTCCTTCACCTTGAAAAATGCGAGAGGCCCGTGGCTTTGCAG
CATTCCCGCCTCTTCACCATCAACCAAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004185	serine-type carboxypeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR001563	Peptidase_S10

Search form