GR404260

EST Overview


Name	GR404260
Unique Name	GR404260
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	344

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR404260 vs. SwissProt
Match: MKS1_ARATH (Protein MKS1 OS=Arabidopsis thaliana GN=MKS1 PE=1 SV=2)

HSP 1 Score: 46.2098 bits (108), Expect = 7.528e-11
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTS 341
            ++IY VSPKV+H T  +FMN+VQRLTG +S
Sbjct:   66 VVIYAVSPKVVHATASEFMNVVQRLTGISS 95

HSP 2 Score: 39.6614 bits (91), Expect = 7.528e-11
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1

Query:  112 STGRSPRRELQ--GPRPTPLRIHKDSHKISK 198
            S  ++ +R+LQ  GPRP+PL +HKDSHKI K
Sbjct:   13 SDQQNQKRQLQICGPRPSPLSVHKDSHKIKK 43

[Close]

BLAST of GR404260 vs. TrEMBL
Match: B9SJG7_RICCO (Protein MKS1, putative OS=Ricinus communis GN=RCOM_0137280 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.129e-15
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSPKVIHT P DFMNLVQRLTGSTSS
Sbjct:   62 VIIYTVSPKVIHTNPSDFMNLVQRLTGSTSS 92

HSP 2 Score: 46.595 bits (109), Expect = 1.129e-15
Identity = 20/31 (64.52%), Postives = 23/31 (74.19%), Query Frame = 1

Query:  106 DISTGRSPRRELQGPRPTPLRIHKDSHKISK 198
            D    RSPR+ELQGPRP  L++ KDSHKI K
Sbjct:    5 DFPVTRSPRKELQGPRPPALKVRKDSHKIKK 35

[Close]

BLAST of GR404260 vs. TrEMBL
Match: A5C6Y5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_029011 PE=4 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 4.190e-15
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 1

Query:  106 DISTGRSPRRELQGPRPTPLRIHKDSHKISK 198
            D S G+SPRREL GPRPTPL++ KDSHKI K
Sbjct:    5 DFSGGKSPRRELLGPRPTPLKVRKDSHKIRK 35

HSP 2 Score: 51.6026 bits (122), Expect = 4.190e-15
Identity = 23/31 (74.19%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSPK+IHT P +FM LVQRLTG +SS
Sbjct:   50 VIIYTVSPKIIHTQPSEFMTLVQRLTGLSSS 80

[Close]

BLAST of GR404260 vs. TrEMBL
Match: B9GTL2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_754475 PE=4 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.622e-14
Identity = 25/31 (80.65%), Postives = 28/31 (90.32%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSPKVIHT P DFM LVQRLTGS+S+
Sbjct:   90 VIIYTVSPKVIHTNPNDFMTLVQRLTGSSST 120

HSP 2 Score: 46.9802 bits (110), Expect = 2.622e-14
Identity = 21/31 (67.74%), Postives = 23/31 (74.19%), Query Frame = 1

Query:  106 DISTGRSPRRELQGPRPTPLRIHKDSHKISK 198
            D    RSPR+ELQGPRP  L+I KDSHKI K
Sbjct:   36 DFPISRSPRKELQGPRPPALKIRKDSHKIRK 66

[Close]

BLAST of GR404260 vs. TrEMBL
Match: B9H7P8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_559348 PE=4 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.440e-14
Identity = 24/31 (77.42%), Postives = 28/31 (90.32%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYT+SPKVIHT P DFM LVQRLTGS+S+
Sbjct:   59 VIIYTLSPKVIHTNPNDFMTLVQRLTGSSST 89

HSP 2 Score: 47.3654 bits (111), Expect = 4.440e-14
Identity = 21/31 (67.74%), Postives = 23/31 (74.19%), Query Frame = 1

Query:  106 DISTGRSPRRELQGPRPTPLRIHKDSHKISK 198
            D    RSPR+ELQGPRP  L+I KDSHKI K
Sbjct:    5 DFPISRSPRKELQGPRPPALKIRKDSHKIKK 35

[Close]

BLAST of GR404260 vs. TrEMBL
Match: B4G0G3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 2.899e-12
Identity = 21/25 (84.00%), Postives = 22/25 (88.00%), Query Frame = 1

Query:  124 SPRRELQGPRPTPLRIHKDSHKISK 198
            S RRELQGPRP PLR+HKDSHKI K
Sbjct:   20 SQRRELQGPRPAPLRLHKDSHKIKK 44

HSP 2 Score: 46.2098 bits (108), Expect = 2.899e-12
Identity = 18/28 (64.29%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGS 335
            +I+YTVSPKV+H  P +FM++VQRLTG+
Sbjct:   63 VIVYTVSPKVVHAKPSEFMSVVQRLTGA 90

[Close]

BLAST of GR404260 vs. TrEMBL
Match: C5YLD9_SORBI (Putative uncharacterized protein Sb07g000390 OS=Sorghum bicolor GN=Sb07g000390 PE=4 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 4.895e-12
Identity = 20/28 (71.43%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGS 335
            +IIYTVSPKV+H  PG+FM++VQRLTG+
Sbjct:   66 VIIYTVSPKVVHAEPGEFMSVVQRLTGA 93

HSP 2 Score: 45.4394 bits (106), Expect = 4.895e-12
Identity = 20/29 (68.97%), Postives = 22/29 (75.86%), Query Frame = 1

Query:  112 STGRSPRRELQGPRPTPLRIHKDSHKISK 198
            S   S RRELQGPRP PL++ KDSHKI K
Sbjct:   18 SPSSSQRRELQGPRPAPLKVRKDSHKIRK 46

[Close]

BLAST of GR404260 vs. TrEMBL
Match: D7KJZ9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472365 PE=4 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.116e-10
Identity = 23/31 (74.19%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSPK+IHT P +FM LVQRLTG TS+
Sbjct:   60 VIIYTVSPKIIHTHPNNFMTLVQRLTGKTST 90

HSP 2 Score: 37.7354 bits (86), Expect = 1.116e-10
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 1

Query:  121 RSPR-RELQGPRPTPLRIHKDSHKISK 198
            RSPR R + GPRPTPL++  DSHKI K
Sbjct:    4 RSPRSRGILGPRPTPLKVRGDSHKIIK 30

[Close]

BLAST of GR404260 vs. TrEMBL
Match: A5AJM8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000273 PE=4 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 5.443e-10
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYT SPK+IHT   DFM LVQ+LTG +SS
Sbjct:   87 VIIYTHSPKIIHTQARDFMALVQKLTGLSSS 117

HSP 2 Score: 42.743 bits (99), Expect = 5.443e-10
Identity = 16/26 (61.54%), Postives = 23/26 (88.46%), Query Frame = 1

Query:  121 RSPRRELQGPRPTPLRIHKDSHKISK 198
            + PR+E+ GPRP+PL+I+KDSH I+K
Sbjct:   41 QQPRKEINGPRPSPLKINKDSHSINK 66

[Close]

BLAST of GR404260 vs. TrEMBL
Match: C0PLR5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 1.181e-9
Identity = 23/31 (74.19%), Postives = 25/31 (80.65%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIY  SPKVIHT PGDFM LVQRLTG  S+
Sbjct:   73 VIIYDASPKVIHTKPGDFMALVQRLTGPGST 103

HSP 2 Score: 36.965 bits (84), Expect = 1.181e-9
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 1

Query:  106 DISTG--RSPR-RELQGPRPTPLRIHKDSHKISK 198
            D S+G   SPR R+LQGPRP  L + KDSHK+ K
Sbjct:    2 DASSGDRSSPRSRQLQGPRPPRLAVSKDSHKVRK 35

[Close]

BLAST of GR404260 vs. TrEMBL
Match: Q9LMP5_ARATH (F16F4.1 protein OS=Arabidopsis thaliana GN=At1g21326 PE=4 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.188e-9
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSP++IHT P +FM LVQRLTG TS+
Sbjct:   61 VIIYTVSPRIIHTHPNNFMTLVQRLTGKTST 91

HSP 2 Score: 35.4242 bits (80), Expect = 1.188e-9
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  121 RSPR-RELQGPRPTPLRIHKDSHKISK 198
            RSPR R + GPRP PL++  DSHKI K
Sbjct:    4 RSPRSRGILGPRPIPLKVRGDSHKIIK 30

[Close]

BLAST of GR404260 vs. TAIR peptide
Match: AT1G21326.1 (| Symbols: | VQ motif-containing protein | chr1:7469002-7469721 REVERSE LENGTH=239)

HSP 1 Score: 50.8322 bits (120), Expect = 4.603e-12
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYTVSP++IHT P +FM LVQRLTG TS+
Sbjct:   61 VIIYTVSPRIIHTHPNNFMTLVQRLTGKTST 91

HSP 2 Score: 35.4242 bits (80), Expect = 4.603e-12
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  121 RSPR-RELQGPRPTPLRIHKDSHKISK 198
            RSPR R + GPRP PL++  DSHKI K
Sbjct:    4 RSPRSRGILGPRPIPLKVRGDSHKIIK 30

[Close]

BLAST of GR404260 vs. TAIR peptide
Match: AT3G18690.1 (| Symbols: MKS1 | MAP kinase substrate 1 | chr3:6429755-6430423 REVERSE LENGTH=222)

HSP 1 Score: 46.2098 bits (108), Expect = 5.990e-12
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTS 341
            ++IY VSPKV+H T  +FMN+VQRLTG +S
Sbjct:   66 VVIYAVSPKVVHATASEFMNVVQRLTGISS 95

HSP 2 Score: 39.6614 bits (91), Expect = 5.990e-12
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1

Query:  112 STGRSPRRELQ--GPRPTPLRIHKDSHKISK 198
            S  ++ +R+LQ  GPRP+PL +HKDSHKI K
Sbjct:   13 SDQQNQKRQLQICGPRPSPLSVHKDSHKIKK 43

[Close]

BLAST of GR404260 vs. TAIR peptide
Match: AT1G21320.1 (| Symbols: | nucleotide binding;nucleic acid binding | chr1:7462834-7465200 REVERSE LENGTH=421)

HSP 1 Score: 46.9802 bits (110), Expect = 6.112e-11
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 3

Query:  258 IYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            IYTV+P++IHT P +FM LVQRLTG TS+
Sbjct:   63 IYTVTPRIIHTHPNNFMTLVQRLTGQTST 91

HSP 2 Score: 35.4242 bits (80), Expect = 6.112e-11
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  121 RSPR-RELQGPRPTPLRIHKDSHKISK 198
            RSPR R   GP+PTPL++  DSHKI K
Sbjct:    4 RSPRSRGNMGPKPTPLKVRGDSHKIIK 30

[Close]

BLAST of GR404260 vs. TAIR peptide
Match: AT3G18360.1 (| Symbols: | VQ motif-containing protein | chr3:6300827-6301684 REVERSE LENGTH=285)

HSP 1 Score: 45.8246 bits (107), Expect = 2.498e-7
Identity = 20/31 (64.52%), Postives = 25/31 (80.65%), Query Frame = 3

Query:  252 IIIYTVSPKVIHTTPGDFMNLVQRLTGSTSS 344
            +IIYT +P++IHT P DFM LVQ+LTG T S
Sbjct:   74 VIIYTHTPRIIHTNPKDFMALVQKLTGMTHS 104

HSP 2 Score: 24.2534 bits (51), Expect = 2.498e-7
Identity = 9/17 (52.94%), Postives = 12/17 (70.59%), Query Frame = 1

Query:  148 PRPTPLRIHKDSHKISK 198
            P P  L+++KDSH I K
Sbjct:   41 PSPPTLKVNKDSHVIKK 57

The following BLAST results are available for this feature:

BLAST of GR404260 vs. SwissProt
BLAST of GR404260 vs. TrEMBL
BLAST of GR404260 vs. TAIR peptide

BLAST of GR404260 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
MKS1_ARATH	7.528e-11	63.33	Protein MKS1 OS=Arabidopsis thaliana GN=MKS1 PE=1 ...	[more]

BLAST of GR404260 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SJG7_RICCO	1.129e-15	90.32	Protein MKS1, putative OS=Ricinus communis GN=RCOM...	[more]
A5C6Y5_VITVI	4.190e-15	74.19	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9GTL2_POPTR	2.622e-14	80.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H7P8_POPTR	4.440e-14	77.42	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B4G0G3_MAIZE	2.899e-12	84.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5YLD9_SORBI	4.895e-12	71.43	Putative uncharacterized protein Sb07g000390 OS=So...	[more]
D7KJZ9_ARALY	1.116e-10	74.19	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A5AJM8_VITVI	5.443e-10	67.74	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C0PLR5_MAIZE	1.181e-9	74.19	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q9LMP5_ARATH	1.188e-9	70.97	F16F4.1 protein OS=Arabidopsis thaliana GN=At1g213...	[more]

BLAST of GR404260 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G21326.1	4.603e-12	70.97	\| Symbols: \| VQ motif-containing protein \| chr1:7...	[more]
AT3G18690.1	5.990e-12	63.33	\| Symbols: MKS1 \| MAP kinase substrate 1 \| chr3:64...	[more]
AT1G21320.1	6.112e-11	68.97	\| Symbols: \| nucleotide binding;nucleic acid bind...	[more]
AT3G18360.1	2.498e-7	64.52	\| Symbols: \| VQ motif-containing protein \| chr3:6...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 41..57 score
None	No IPR available	SEG	seg	seg	coord: 34..55 score
IPR008889	VQ	PFAM	PF05678	VQ	coord: 59..85 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR404260 ID=GR404260; Name=GR404260; organism=Cicer arietinum; type=EST; length=344bp
GACTCCTCCATACAATAATACTATTCACACCCCCTTTCTCTTCTAATCAC
AATTTCAATTTCATTTTTTTTAACATAAATATATAGCAACATGAATCACT
TGCCAGACATTTCTACCGGAAGATCGCCAAGAAGAGAGCTTCAAGGCCCA
CGACCTACTCCTCTAAGAATACACAAAGACTCTCACAAGATCAGCAAAAA
CCACCGTTGCCGCCGCCACAACAACAACAACAACAACCACCACGTCAACC
AATAATAATATACACCGTATCTCCTAAGGTAATTCACACCACACCTGGTG
ACTTCATGAACCTCGTTCAACGTCTAACCGGCTCAACATCATCA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR008889	VQ

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