GR407971

EST Overview


Name	GR407971
Unique Name	GR407971
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	595

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR407971 vs. SwissProt
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.808e-11
Identity = 51/152 (33.55%), Postives = 69/152 (45.39%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNEL-GEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLP-RSSDVSIFDTCYDLYGFVS-VRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GL VG  R+PI    F LN   G GG+++D+GT +T     AY + R  FI Q  NLP  +   S FD C+      S +++PT   +F GG  L LP+ N+ I  ++ G  C A   S  G+               D  N  V F    C
Sbjct:  286 GLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGD-LELPSENYFISPSN-GLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

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BLAST of GR407971 vs. SwissProt
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.180e-9
Identity = 46/118 (38.98%), Postives = 62/118 (52.54%), Query Frame = -1

Query:  248 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSI-FDTCYDLYGFVS-VRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPS 595
            G+ VGG  + I    F+L + G GG+++D+GT +T LP  AY+A   AF  Q  NLP   + S    TC+      S V+VP IS  F GG +L L  +N LI   + G  C A   S
Sbjct:  287 GITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISPAE-GVICLAMGSS 401

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BLAST of GR407971 vs. TrEMBL
Match: Q8LPE4_CICAR (Putative nucleoid DNA-binding protein (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.410e-68
Identity = 130/144 (90.28%), Postives = 131/144 (90.97%), Query Frame = -1

Query:  149 GIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 580
            G+RVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGL             SVDGVNGFVGFGPNIC
Sbjct:    1 GVRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLSIIGNIQQEGIEISVDGVNGFVGFGPNIC 144

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BLAST of GR407971 vs. TrEMBL
Match: B9RGI0_RICCO (Aspartic proteinase nepenthesin-1, putative OS=Ricinus communis GN=RCOM_1454130 PE=4 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 1.082e-60
Identity = 115/149 (77.18%), Postives = 126/149 (84.56%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+GVGG++VPISEDVF+LNE+G GGVVMDTGTAVTR+PT AY AFRDAFIGQT NLPR+S VSIFDTCY+L GFVSVRVPT+SFYF GGPILTLPARNFLIPV+DVGTFCFAFA SPSGL             S DG NGFVGFGPN+C
Sbjct:  333 GVGVGGMKVPISEDVFQLNEMGNGGVVMDTGTAVTRIPTVAYVAFRDAFIGQTGNLPRASGVSIFDTCYNLNGFVSVRVPTVSFYFAGGPILTLPARNFLIPVDDVGTFCFAFAASPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 481

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BLAST of GR407971 vs. TrEMBL
Match: D7TXZ2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_71.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034372001 PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.112e-60
Identity = 114/149 (76.51%), Postives = 124/149 (83.22%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVGGIRVPISE+VFRL ELG+GGVVMDTGTAVTRLPT AY AFRDAF+ QT NLPR++ V+IFDTCYDL GFVSVRVPT+SFYF GGPILTLPARNFLIP++D GTFCFAFAPS SGL             S DG NG+VGFGPNIC
Sbjct:  373 GLGVGGIRVPISEEVFRLTELGDGGVVMDTGTAVTRLPTLAYQAFRDAFLAQTANLPRATGVAIFDTCYDLLGFVSVRVPTVSFYFSGGPILTLPARNFLIPMDDAGTFCFAFAPSTSGLSILGNIQQEGIQISFDGANGYVGFGPNIC 521

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BLAST of GR407971 vs. TrEMBL
Match: A5BAQ3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022007 PE=4 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 5.938e-59
Identity = 112/147 (76.19%), Postives = 122/147 (82.99%), Query Frame = -1

Query:  149 GVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 589
            GVGGIRVPISE+VFRL ELG+GGVVMDTGTAVTRLPT AY AFRDAF+ QT NLPR++ V+IFDTCYDL GFVSVRVPT+SFYF GGPILTLPARNFLIP++D GTFCFAFAPS SGL             S DG NG+VGFGPNIC
Sbjct:    4 GVGGIRVPISEEVFRLTELGDGGVVMDTGTAVTRLPTLAYQAFRDAFLAQTANLPRATGVAIFDTCYDLLGFVSVRVPTVSFYFSGGPILTLPARNFLIPMDDAGTFCFAFAPSTSGLSILGNIQQEGIQISFDGANGYVGFGPNIC 150

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BLAST of GR407971 vs. TrEMBL
Match: B9H484_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_207052 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.013e-58
Identity = 111/149 (74.50%), Postives = 122/149 (81.88%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVG ++VPISED+F L ELG GGVVMDTGTAVTR PT AY+AFRDAFI QT NLPR+S VSIFDTCY+L+GF+SVRVPT+SFYF GGPILTLPA NFLIPV+D GTFCFAFAPSPSGL             SVDG N FVGFGPN+C
Sbjct:  234 GLGVGDMKVPISEDIFELTELGNGGVVMDTGTAVTRFPTVAYEAFRDAFIDQTGNLPRASGVSIFDTCYNLFGFLSVRVPTVSFYFSGGPILTLPANNFLIPVDDAGTFCFAFAPSPSGLSILGNIQQEGIQISVDGANEFVGFGPNVC 382

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BLAST of GR407971 vs. TrEMBL
Match: B9HER8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_764020 PE=4 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 5.027e-58
Identity = 111/149 (74.50%), Postives = 122/149 (81.88%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVG  RVP+SEDVF+LNELG GGVVMDTGTAVTR PT AY+AFR+AFI QT NLPR+S VSIFDTCY+L+GF+SVRVPT+SFYF GGPILT+PA NFLIPV+D GTFCFAFAPSPSGL             SVD  N FVGFGPNIC
Sbjct:  234 GLGVGDTRVPVSEDVFQLNELGSGGVVMDTGTAVTRFPTVAYEAFRNAFIEQTQNLPRASGVSIFDTCYNLFGFLSVRVPTVSFYFSGGPILTIPANNFLIPVDDAGTFCFAFAPSPSGLSILGNIQQEGIQISVDEANEFVGFGPNIC 382

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BLAST of GR407971 vs. TrEMBL
Match: Q9LHE3_ARATH (Nucleoid chloroplast DNA-binding protein-like OS=Arabidopsis thaliana GN=At3g20015 PE=2 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 3.983e-55
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVGG+R+P+ + VF L E G+GGVVMDTGTAVTRLPTAAY AFRD F  QT NLPR+S VSIFDTCYDL GFVSVRVPT+SFYF  GP+LTLPARNFL+PV+D GT+CFAFA SP+GL             S DG NGFVGFGPN+C
Sbjct:  322 GLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

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BLAST of GR407971 vs. TrEMBL
Match: D7LAV2_ARALY (Pepsin A OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_898406 PE=4 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 8.873e-55
Identity = 104/149 (69.80%), Postives = 117/149 (78.52%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVGG+R+P+ + VF L E G+GGVVMDTGTAVTRLPT AY AFRD F  QT NLPR+S VSIFDTCYDL GFVSVRVPT+SFYF  GP+LTLPARNFL+PV+D GT+CFAFA SP+GL             S DG NGFVGFGPN+C
Sbjct:  323 GLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTGAYAAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 471

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BLAST of GR407971 vs. TrEMBL
Match: Q7XKB9_ORYSJ (OSJNBa0064G10.12 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0064G10.12 PE=4 SV=2)

HSP 1 Score: 168.318 bits (425), Expect = 3.619e-40
Identity = 86/149 (57.72%), Postives = 100/149 (67.11%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+GVGG R+P+ + +F+L E G GGVVMDTGTAVTRLP  AY A R AF G    LPRS  VS+ DTCYDL G+ SVRVPT+SFYF  G +LTLPARN L+ V     FC AFAPS SG+             +VD  NG+VGFGPN C
Sbjct:  304 GIGVGGERLPLQDSLFQLTEDGAGGVVMDTGTAVTRLPREAYAALRGAFDGAMGALPRSPAVSLLDTCYDLSGYASVRVPTVSFYFDQGAVLTLPARNLLVEVGG-AVFCLAFAPSSSGISILGNIQQEGIQITVDSANGYVGFGPNTC 451

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BLAST of GR407971 vs. TrEMBL
Match: Q0J912_ORYSJ (Os04g0677100 protein OS=Oryza sativa subsp. japonica GN=Os04g0677100 PE=4 SV=2)

HSP 1 Score: 168.318 bits (425), Expect = 3.619e-40
Identity = 86/149 (57.72%), Postives = 100/149 (67.11%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+GVGG R+P+ + +F+L E G GGVVMDTGTAVTRLP  AY A R AF G    LPRS  VS+ DTCYDL G+ SVRVPT+SFYF  G +LTLPARN L+ V     FC AFAPS SG+             +VD  NG+VGFGPN C
Sbjct:  317 GIGVGGERLPLQDSLFQLTEDGAGGVVMDTGTAVTRLPREAYAALRGAFDGAMGALPRSPAVSLLDTCYDLSGYASVRVPTVSFYFDQGAVLTLPARNLLVEVGG-AVFCLAFAPSSSGISILGNIQQEGIQITVDSANGYVGFGPNTC 464

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BLAST of GR407971 vs. TAIR peptide
Match: AT3G20015.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:6978746-6980158 REVERSE LENGTH=470)

HSP 1 Score: 218.009 bits (554), Expect = 3.003e-57
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            GLGVGG+R+P+ + VF L E G+GGVVMDTGTAVTRLPTAAY AFRD F  QT NLPR+S VSIFDTCYDL GFVSVRVPT+SFYF  GP+LTLPARNFL+PV+D GT+CFAFA SP+GL             S DG NGFVGFGPN+C
Sbjct:  322 GLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

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BLAST of GR407971 vs. TAIR peptide
Match: AT3G18490.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:6349090-6350592 REVERSE LENGTH=500)

HSP 1 Score: 140.198 bits (352), Expect = 7.958e-34
Identity = 70/150 (46.67%), Postives = 95/150 (63.33%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPR-SSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G  VGG +V + + +F ++  G GGV++D GTAVTRL T AY++ RDAF+  T NL + SS +S+FDTCYD     +V+VPT++F+F GG  L LPA+N+LIPV+D GTFCFAFAP+ S L             + D     +G   N C
Sbjct:  351 GFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

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BLAST of GR407971 vs. TAIR peptide
Match: AT1G25510.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:8959372-8960823 REVERSE LENGTH=483)

HSP 1 Score: 139.813 bits (351), Expect = 1.039e-33
Identity = 65/149 (43.62%), Postives = 97/149 (65.10%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+ VGG  + I +  F ++E G GG+++D+GTAVTRL T  Y++ RD+F+  T +L +++ V++FDTCY+L    +V VPT++F+F GG +L LPA+N++IPV+ VGTFC AFAP+ S L             + D  N  +GF  N C
Sbjct:  335 GISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

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BLAST of GR407971 vs. TAIR peptide
Match: AT3G61820.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:22880074-22881525 REVERSE LENGTH=483)

HSP 1 Score: 128.642 bits (322), Expect = 2.396e-30
Identity = 71/150 (47.33%), Postives = 91/150 (60.67%), Query Frame = -1

Query:  149 GLGVGGIRVP-ISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+ VGG RVP +SE  F+L+  G GGV++D+GT+VTRL   AY A RDAF    T L R+   S+FDTC+DL G  +V+VPT+ F+F GG + +LPA N+LIPVN  G FCFAFA +   L             + D V   VGF    C
Sbjct:  335 GISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

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BLAST of GR407971 vs. TAIR peptide
Match: AT1G01300.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:117065-118522 FORWARD LENGTH=485)

HSP 1 Score: 123.25 bits (308), Expect = 1.007e-28
Identity = 66/150 (44.00%), Postives = 90/150 (60.00%), Query Frame = -1

Query:  149 GLGVGGIRVP-ISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+ VGG RVP ++  +F+L+++G GGV++D+GT+VTRL   AY A RDAF      L R+ D S+FDTC+DL     V+VPT+  +F G  + +LPA N+LIPV+  G FCFAFA +  GL               D  +  VGF P  C
Sbjct:  336 GISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADV-SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of GR407971 vs. TAIR peptide
Match: AT5G10770.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr5:3403331-3405331 REVERSE LENGTH=474)

HSP 1 Score: 91.6633 bits (226), Expect = 3.246e-19
Identity = 54/148 (36.49%), Postives = 75/148 (50.68%), Query Frame = -1

Query:  149 VGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFA--PSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 586
            VGG ++PI   VF        G ++D+GT +TRLP  AY A R +F  + +  P +S VSI DTC+DL GF +V +P ++F F GG ++ L ++  +  V  +   C AFA     S                 DG  G VGF PN C
Sbjct:  332 VGGQKLPIPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

[Close]

BLAST of GR407971 vs. TAIR peptide
Match: AT5G10760.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr5:3400671-3402165 REVERSE LENGTH=464)

HSP 1 Score: 80.4925 bits (197), Expect = 7.483e-16
Identity = 47/149 (31.54%), Postives = 65/149 (43.62%), Query Frame = -1

Query:  149 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSPSGLXXXXXXXXXXXXXSVDGVNGFVGFGPNIC 595
            G+ + GI V   E     N     G ++D+GT  TRLPT  Y   R  F  + ++   +S   +FDTCYD  G  +V  PTI+F F G  ++ L      +P+  +   C AFA +                   D   G VGF PN C
Sbjct:  317 GIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIK-ISQVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of GR407971 vs. TAIR peptide
Match: AT2G03200.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr2:966506-967891 REVERSE LENGTH=461)

HSP 1 Score: 61.6178 bits (148), Expect = 3.597e-10
Identity = 34/117 (29.06%), Postives = 58/117 (49.57%), Query Frame = -1

Query:  248 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFV-SVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPS 595
            G+ VG  R+ + +  F L E G GG+++D+GT +T L   A+   ++ F  + +     S  +  D C+ L     ++ VP + F+F G   L LP  N+++  +  G  C A   S
Sbjct:  311 GITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGAD-LELPGENYMVADSSTGVLCLAMGSS 426

[Close]

BLAST of GR407971 vs. TAIR peptide
Match: AT1G79720.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr1:29997259-29998951 REVERSE LENGTH=484)

HSP 1 Score: 58.151 bits (139), Expect = 3.977e-9
Identity = 34/115 (29.57%), Postives = 53/115 (46.09%), Query Frame = -1

Query:  254 GLGVGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQTTNLPRSSDVSIFDTCYDLYGFVSVRVPTISFYFLGGPILTLPARN-FLIPVNDVGTFCFAFA 595
            G  +GG+ +  S         G G +++D+GT +TRLP + Y A +  F+ Q +  P +   SI DTC++L  +  + +P I   F G   L +     F     D    C A A
Sbjct:  339 GASIGGVELKSSS-------FGRG-ILIDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTCFNLTSYEDISIPIIKMIFQGNAELEVDVTGVFYFVKPDASLVCLALA 445

[Close]

BLAST of GR407971 vs. TAIR peptide
Match: AT2G42980.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr2:17875005-17876588 REVERSE LENGTH=527)

HSP 1 Score: 55.0694 bits (131), Expect = 3.367e-8
Identity = 30/117 (25.64%), Postives = 59/117 (50.43%), Query Frame = -1

Query:  245 VGGIRVPISEDVFRLNELGEGGVVMDTGTAVTRLPTAAYDAFRDAFIGQ-TTNLPRSSDVSIFDTCYDLYGFV--SVRVPTISFYFLGGPILTLPARNFLIPVNDVGTFCFAFAPSP 586
            VGG  + I E+ + ++  G+GG ++D+GT ++     AY+  ++ F  +   N P   D  + D C+++ G    ++ +P +   F+ G +   PA N  I +++    C A   +P
Sbjct:  376 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSE-DLVCLAILGTP 491

The following BLAST results are available for this feature:

BLAST of GR407971 vs. SwissProt
BLAST of GR407971 vs. TrEMBL
BLAST of GR407971 vs. TAIR peptide

BLAST of GR407971 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
NEP1_NEPGR	2.808e-11	33.55	Aspartic proteinase nepenthesin-1 OS=Nepenthes gra...	[more]
NEP2_NEPGR	1.180e-9	38.98	Aspartic proteinase nepenthesin-2 OS=Nepenthes gra...	[more]

BLAST of GR407971 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8LPE4_CICAR	1.410e-68	90.28	Putative nucleoid DNA-binding protein (Fragment) O...	[more]
B9RGI0_RICCO	1.082e-60	77.18	Aspartic proteinase nepenthesin-1, putative OS=Ric...	[more]
D7TXZ2_VITVI	4.112e-60	76.51	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BAQ3_VITVI	5.938e-59	76.19	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9H484_POPTR	1.013e-58	74.50	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9HER8_POPTR	5.027e-58	74.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9LHE3_ARATH	3.983e-55	70.47	Nucleoid chloroplast DNA-binding protein-like OS=A...	[more]
D7LAV2_ARALY	8.873e-55	69.80	Pepsin A OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]
Q7XKB9_ORYSJ	3.619e-40	57.72	OSJNBa0064G10.12 protein OS=Oryza sativa subsp. ja...	[more]
Q0J912_ORYSJ	3.619e-40	57.72	Os04g0677100 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of GR407971 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G20015.1	3.003e-57	70.47	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G18490.1	7.958e-34	46.67	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G25510.1	1.039e-33	43.62	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT3G61820.1	2.396e-30	47.33	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G01300.1	1.007e-28	44.00	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT5G10770.1	3.246e-19	36.49	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT5G10760.1	7.483e-16	31.54	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT2G03200.1	3.597e-10	29.06	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT1G79720.1	3.977e-9	29.57	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]
AT2G42980.1	3.367e-8	25.64	\| Symbols: \| Eukaryotic aspartyl protease family ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Peptidase A1	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 1..149 score: 2
IPR009007	Peptidase aspartic, catalytic	GENE3D	2.40.70.10	no description	coord: 2..149 score: 1.6
IPR021109	Peptidase aspartic	SUPERFAMILY	50630	Acid proteases	coord: 1..149 score: 7.5
None	No IPR available	PANTHER	PTHR13683:SF98	CHLOROPLAST NUCLEIOD DNA-BINDING-RELATED	coord: 1..149 score: 2
None	No IPR available	SEG	seg	seg	coord: 121..134 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR407971 ID=GR407971; Name=GR407971; organism=Cicer arietinum; type=EST; length=595bp
AGAACATTTAATGACCACATGCATGGCCTAATTATTTCATTTATCTATAT
AGTACGATTATGTGACAATTTTTTTAATGATAAATTATGTGACATTACTA
ACACTAACAAAATTAAGACATAATATACACACGGTTACATAACACTTAAC
ATATATTGGGTCCAAATCCTACAAACCCATTAACTCCATCGACTGAAATT
TCAATCCCTTCTTGCTGGATGTTCCCTATAATGGAAAGTCCTGAAGGAGA
AGGAGCAAATGCAAAGCAGAAAGTTCCAACATCATTAACTGGAATTAAGA
AGTTCCTAGCTGGAAGGGTCAATATAGGCCCACCCAAGAAATAAAATGAT
ATGGTTGGTACCCGAACCGACACAAACCCGTATAAATCATAACACGTGTC
AAAAATGGATACGTCCGATGACCGGGGTAGGTTTGTGGTTTGACCAATAA
AAGCATCTCGAAAAGCGTCGTAAGCCGCCGTTGGCAGCCTCGTCACGGCA
GTTCCGGTGTCCATCACCACCCCACCCTCACCTAATTCATTTAACCGAAA
AACATCCTCGGATATAGGTACTCTAATTCCCCCAACTCCAAGACC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR009007	Peptidase aspartic, catalytic
IPR021109	Peptidase_aspartic_dom_sf

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