GR913036

EST Overview


Name	GR913036
Unique Name	GR913036
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	458

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR913036 vs. SwissProt
Match: RPB4_MOUSE (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus GN=Polr2d PE=2 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 5.421e-14
Identity = 38/96 (39.58%), Postives = 64/96 (66.67%), Query Frame = 3

Query:  171 SGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            +G+ EE+A++L    EF  A+ L+N EV ++L+H+ +Q + + D+   ++S+VF K+L Y  RFSR+KN + +  VR +L + +L +FEL  L NL
Sbjct:   10 AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANL 103

[Close]

BLAST of GR913036 vs. SwissProt
Match: RPB4_HUMAN (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens GN=POLR2D PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 5.421e-14
Identity = 38/96 (39.58%), Postives = 64/96 (66.67%), Query Frame = 3

Query:  171 SGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            +G+ EE+A++L    EF  A+ L+N EV ++L+H+ +Q + + D+   ++S+VF K+L Y  RFSR+KN + +  VR +L + +L +FEL  L NL
Sbjct:   10 AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANL 103

[Close]

BLAST of GR913036 vs. SwissProt
Match: RPB4_DICDI (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum GN=polr2d PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 5.610e-11
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 3

Query:  165 KMSG-EEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDD-------------PMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            K SG +EEE+   LK   +   AK L+N EV+++L+H+    +    +              +N + + F K+L Y ++FSRYKN  +++QVR  L++  L EFE+  L NL
Sbjct:    7 KRSGIQEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSINDIIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANL 118

[Close]

BLAST of GR913036 vs. SwissProt
Match: RPB4_DROME (DNA-directed RNA polymerase II 16 kDa polypeptide OS=Drosophila melanogaster GN=Rpb4 PE=1 SV=5)

HSP 1 Score: 65.4698 bits (158), Expect = 1.250e-10
Identity = 34/92 (36.96%), Postives = 55/92 (59.78%), Query Frame = 3

Query:  183 EENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            +E+AA+L+   EF  A+ L+  EV ++LDH+  + Q  S D   + S+VF K+  Y   F ++KN + +   R +L + +L +FEL  LGNL
Sbjct:   11 DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSFRKFKNKETIMSARSLLMQKKLHKFELAALGNL 100

[Close]

BLAST of GR913036 vs. TrEMBL
Match: C6T3Y7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.417e-47
Identity = 95/97 (97.94%), Postives = 97/97 (100.00%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HKYEQLQQ+SDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQTSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: C6SV83_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.850e-47
Identity = 94/97 (96.91%), Postives = 97/97 (100.00%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HKYEQLQQ+SDDPMNQ+SQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQTSDDPMNQISQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: C6T558_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 7.032e-47
Identity = 93/97 (95.88%), Postives = 97/97 (100.00%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEV+LIL+HKYEQLQQ+SDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARY+LAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQTSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYELAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: B9H5K4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_650821 PE=4 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.672e-46
Identity = 92/97 (94.85%), Postives = 96/97 (98.97%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGD+FLKAKCLMNCEV+LIL+HKYEQLQQ SDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+RYQLAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDDFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: A5APH2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_017448 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 5.953e-46
Identity = 91/97 (93.81%), Postives = 96/97 (98.97%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEV++IL+HKYEQLQQ SDDP+NQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+RYQLAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVAVILEHKYEQLQQMSDDPLNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: B9S7P5_RICCO (DNA-directed RNA polymerase II, putative OS=Ricinus communis GN=RCOM_0610380 PE=4 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.015e-45
Identity = 91/97 (93.81%), Postives = 96/97 (98.97%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LIL+HKYEQLQQ S+DPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+RYQLAEFELCVLGNL
Sbjct:    1 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSNDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: O48890_ARATH (15.9 kDa subunit of RNA polymerase II OS=Arabidopsis thaliana GN=RPB15.9 PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.581e-45
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HK+EQLQQ S+DPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+R+QL EFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: D7M288_ARALY (RPB15.9 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487817 PE=4 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.581e-45
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HK+EQLQQ S+DPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+R+QL EFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQVSEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TrEMBL
Match: B7U9V3_CARAS (AT5G09920-like protein OS=Cardaminopsis arenosa PE=4 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.581e-45
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HK+EQLQQ S+DPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+R+QL EFELCVLGNL
Sbjct:  148 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQVSEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNL 244

[Close]

BLAST of GR913036 vs. TrEMBL
Match: A5HSG3_ARTAN (Putative 15.9 kDa subunit of RNA polymerase II OS=Artemisia annua PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.501e-44
Identity = 90/97 (92.78%), Postives = 93/97 (95.88%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHK EQLQ  SDDP NQVSQVFEKSLQYVKRFSRYKNPDAVRQVRE+L+R+QLAEFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKLEQLQAMSDDPSNQVSQVFEKSLQYVKRFSRYKNPDAVRQVRELLSRHQLAEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TAIR peptide
Match: AT5G09920.1 (| Symbols: RPB15.9, ATRPB15.9, RPB15.9.9, NRPB4 | RNA polymerase II, Rpb4, core protein | chr5:3096276-3097370 FORWARD LENGTH=138)

HSP 1 Score: 183.341 bits (464), Expect = 4.916e-47
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3

Query:  168 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGNL 458
            MSGEEEENAAELKIGDEFLKAKCLMNCEVSLIL+HK+EQLQQ S+DPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREIL+R+QL EFELCVLGNL
Sbjct:    1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNL 97

[Close]

BLAST of GR913036 vs. TAIR peptide
Match: AT4G15950.1 (| Symbols: NRPD4, NRPE4, RDM2 | RNA polymerase II, Rpb4, core protein | chr4:9040743-9041994 FORWARD LENGTH=205)

HSP 1 Score: 59.6918 bits (143), Expect = 8.198e-10
Identity = 29/76 (38.16%), Postives = 47/76 (61.84%), Query Frame = 3

Query:  228 AKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILARYQLAEFELCVLGN 455
            A+C+M+CE   ILD    QL   S+DP  ++   ++++L YV+    Y NP +VR+V E L  Y +++ E+CV+ N
Sbjct:   89 AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEPLKTYGISDGEMCVIAN 164

The following BLAST results are available for this feature:

BLAST of GR913036 vs. SwissProt
BLAST of GR913036 vs. TrEMBL
BLAST of GR913036 vs. TAIR peptide

BLAST of GR913036 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
RPB4_MOUSE	5.421e-14	39.58	DNA-directed RNA polymerase II subunit RPB4 OS=Mus...	[more]
RPB4_HUMAN	5.421e-14	39.58	DNA-directed RNA polymerase II subunit RPB4 OS=Hom...	[more]
RPB4_DICDI	5.610e-11	33.93	DNA-directed RNA polymerase II subunit rpb4 OS=Dic...	[more]
RPB4_DROME	1.250e-10	36.96	DNA-directed RNA polymerase II 16 kDa polypeptide ...	[more]

BLAST of GR913036 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T3Y7_SOYBN	1.417e-47	97.94	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SV83_SOYBN	1.850e-47	96.91	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T558_SOYBN	7.032e-47	95.88	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9H5K4_POPTR	2.672e-46	94.85	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5APH2_VITVI	5.953e-46	93.81	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9S7P5_RICCO	1.015e-45	93.81	DNA-directed RNA polymerase II, putative OS=Ricinu...	[more]
O48890_ARATH	6.581e-45	92.78	15.9 kDa subunit of RNA polymerase II OS=Arabidops...	[more]
D7M288_ARALY	6.581e-45	92.78	RPB15.9 OS=Arabidopsis lyrata subsp. lyrata GN=ARA...	[more]
B7U9V3_CARAS	6.581e-45	92.78	AT5G09920-like protein OS=Cardaminopsis arenosa PE...	[more]
A5HSG3_ARTAN	2.501e-44	92.78	Putative 15.9 kDa subunit of RNA polymerase II OS=...	[more]

BLAST of GR913036 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G09920.1	4.916e-47	92.78	\| Symbols: RPB15.9, ATRPB15.9, RPB15.9.9, NRPB4 \| ...	[more]
AT4G15950.1	8.198e-10	38.16	\| Symbols: NRPD4, NRPE4, RDM2 \| RNA polymerase II,...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005574	RNA polymerase II, Rpb4	PFAM	PF03874	RNA_pol_Rpb4	coord: 24..98 score: 9.3
IPR006590	RNA polymerase II, Rpb4, core	SMART	SM00657	no description	coord: 17..98 score: 6.3
IPR010997	HRDC-like	SUPERFAMILY	47819	HRDC-like	coord: 1..98 score: 2.9
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 1..98 score: 1.3
None	No IPR available	SEG	seg	seg	coord: 34..51 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR913036 ID=GR913036; Name=GR913036; organism=Cicer arietinum; type=EST; length=458bp
GATTAGCGTGGTCGCGGCCGAGGTCTTCTGTTCCTCCTCCTCCAACGCCG
AGTTTAACAGTTCACGAAATCCAAACTAGGGTTCTGTTCCCCGTGCTCAA
CGAAACAAAATCGGAGACGGTAATCGCCTGAAACCAGCATAGTAACTGAA
TCGTAATCTTCTAAAAAATGTCTGGGGAAGAGGAAGAAAACGCCGCAGAG
CTCAAAATCGGAGACGAGTTTTTGAAAGCAAAGTGCTTGATGAACTGTGA
AGTTTCGTTAATTCTGGACCACAAATATGAGCAGCTTCAGCAGTCGTCTG
ATGATCCCATGAATCAAGTTTCTCAGGTATTTGAAAAGTCATTGCAGTAT
GTGAAGCGTTTCAGTCGCTATAAAAATCCTGATGCTGTTAGACAAGTTCG
AGAAATCCTTGCTAGATATCAGTTGGCCGAGTTTGAGCTGTGCGTCCTAG
GCAATCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0000166	nucleotide binding
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription
GO:0044237	cellular metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR005574	Rpb4/RPC9
IPR006590	RNA_pol_Rpb4/RPC9_core
IPR010997	HRDC-like_sf

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