GR916772

EST Overview


Name	GR916772
Unique Name	GR916772
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	201

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916772 vs. SwissProt
Match: C93C1_SOYBN (2-hydroxyisoflavanone synthase OS=Glycine max GN=IFS2 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.995e-19
Identity = 46/59 (77.97%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            +TEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLK L +G+Y+K+I
Sbjct:  175 LTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKHLKVGKYEKRI 233

[Close]

BLAST of GR916772 vs. SwissProt
Match: C93B1_GLYEC (Licodione synthase OS=Glycyrrhiza echinata GN=CYP93B1 PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 3.412e-11
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            +TEELLK TN+ IS MM+GEAEE RD+ RDV +IFGE +++D I   KK++L  + K+I
Sbjct:  171 ITEELLKLTNNVISIMMVGEAEEARDVVRDVTEIFGEFNVSDFIWLFKKMDLQGFGKRI 229

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9SXS3_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-8 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 8.678e-21
Identity = 51/59 (86.44%), Postives = 55/59 (93.22%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTISRMMLGEAEE RDIARDVLKIFGE+SLTD I PLKKL +GQY+K+I
Sbjct:  175 VTEELLKWTNSTISRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKLKVGQYEKRI 233

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9XGL7_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp93C3 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.133e-20
Identity = 51/59 (86.44%), Postives = 55/59 (93.22%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTISRMMLGEAEE RDIARDVLKIFGE+SLTD I PLKKL +G+Y+KKI
Sbjct:  176 VTEELLKWTNSTISRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKLKVGKYEKKI 234

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q3HNP6_ASTMO (Isoflavone synthase (Fragment) OS=Astragalus mongholicus GN=IFS PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.525e-20
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTISRMMLGEAEE RDIARDVLKIFGE+SLTD I PLKK  +GQY+K+I
Sbjct:   96 VTEELLKWTNSTISRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKFKVGQYEKRI 154

[Close]

BLAST of GR916772 vs. TrEMBL
Match: A5LGW8_LOTJA (2-hydroxyisoflavanone synthase OS=Lotus japonicus GN=IFS2 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.625e-20
Identity = 49/59 (83.05%), Postives = 55/59 (93.22%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTN+TISRMMLGEAEE RDIAR+VLKIFGE+SLTD I PLKKL +GQY+K+I
Sbjct:  175 VTEELLKWTNNTISRMMLGEAEEVRDIAREVLKIFGEYSLTDFIWPLKKLKVGQYEKRI 233

[Close]

BLAST of GR916772 vs. TrEMBL
Match: B2MU03_VIGUN (Isoflavone synthase 1 OS=Vigna unguiculata PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.637e-19
Identity = 49/59 (83.05%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLKKL  GQY+K+I
Sbjct:  175 VTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKKLKFGQYEKRI 233

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9MBE5_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.219e-19
Identity = 47/59 (79.66%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTISRMMLGEAE  +DI R+VLKIFGE+SLTD I PLKKL +GQY+K+I
Sbjct:  175 VTEELLKWTNSTISRMMLGEAEHVKDIVREVLKIFGEYSLTDFIWPLKKLRVGQYEKRI 233

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9M6D6_SOYBN (Isoflavone synthase OS=Glycine max GN=ifs1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.363e-18
Identity = 47/59 (79.66%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLK L +G+Y+K+I
Sbjct:  175 VTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKYLKVGKYEKRI 233

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9M6D5_MEDSA (Isoflavone synthase 1 (Fragment) OS=Medicago sativa GN=ifs1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.363e-18
Identity = 47/59 (79.66%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLK L +G+Y+K+I
Sbjct:  161 VTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKYLKVGKYEKRI 219

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9M6D1_LENCU (Isoflavone synthase 1 (Fragment) OS=Lens culinaris GN=ifs1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.363e-18
Identity = 47/59 (79.66%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLK L +G+Y+K+I
Sbjct:  161 VTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKYLKVGKYEKRI 219

[Close]

BLAST of GR916772 vs. TrEMBL
Match: Q9M6C5_PEA (Isoflavone synthase 1 (Fragment) OS=Pisum sativum GN=ifs1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.363e-18
Identity = 47/59 (79.66%), Postives = 53/59 (89.83%), Query Frame = 1

Query:   25 VTEELLKWTNSTISRMMLGEAEEXRDIARDVLKIFGEHSLTDSICPLKKLNLGQYDKKI 201
            VTEELLKWTNSTIS MMLGEAEE RDIAR+VLKIFGE+SLTD I PLK L +G+Y+K+I
Sbjct:  175 VTEELLKWTNSTISMMMLGEAEEIRDIAREVLKIFGEYSLTDFIWPLKYLKVGKYEKRI 233

The following BLAST results are available for this feature:

BLAST of GR916772 vs. SwissProt
BLAST of GR916772 vs. TrEMBL

BLAST of GR916772 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
C93C1_SOYBN	1.995e-19	77.97	2-hydroxyisoflavanone synthase OS=Glycine max GN=I...	[more]
C93B1_GLYEC	3.412e-11	55.93	Licodione synthase OS=Glycyrrhiza echinata GN=CYP9...	[more]

BLAST of GR916772 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SXS3_GLYEC	8.678e-21	86.44	Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-...	[more]
Q9XGL7_CICAR	1.133e-20	86.44	Cytochrome P450 OS=Cicer arietinum GN=cyp93C3 PE=2...	[more]
Q3HNP6_ASTMO	2.525e-20	84.75	Isoflavone synthase (Fragment) OS=Astragalus mongh...	[more]
A5LGW8_LOTJA	5.625e-20	83.05	2-hydroxyisoflavanone synthase OS=Lotus japonicus ...	[more]
B2MU03_VIGUN	1.637e-19	83.05	Isoflavone synthase 1 OS=Vigna unguiculata PE=2 SV...	[more]
Q9MBE5_LOTJA	6.219e-19	79.66	Cytochrome P450 OS=Lotus japonicus PE=2 SV=1	[more]
Q9M6D6_SOYBN	2.363e-18	79.66	Isoflavone synthase OS=Glycine max GN=ifs1 PE=2 SV...	[more]
Q9M6D5_MEDSA	2.363e-18	79.66	Isoflavone synthase 1 (Fragment) OS=Medicago sativ...	[more]
Q9M6D1_LENCU	2.363e-18	79.66	Isoflavone synthase 1 (Fragment) OS=Lens culinaris...	[more]
Q9M6C5_PEA	2.363e-18	79.66	Isoflavone synthase 1 (Fragment) OS=Pisum sativum ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 7..65 score: 1
None	No IPR available	PANTHER	PTHR19383:SF172	CYTOCHROME P450, SUBFAMILY 93	coord: 7..65 score: 1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916772 ID=GR916772; Name=GR916772; organism=Cicer arietinum; type=EST; length=201bp
GATTAGCGTGGTCGCGGCCGAGGTGTGACAGAGGAACTTTTGAAATGGAC
AAATAGCACTATTTCTAGGATGATGTTGGGTGAGGCTGAAGAANTTAGAG
ATATTGCTCGTGATGTTCTTAAGATTTTTGGGGAGCATAGCCTTACTGAC
TCCATTTGCCCTTTGAAAAAGCTCAACCTTGGCCAATATGACAAGAAGAT
T

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

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