GR917586

EST Overview


Name	GR917586
Unique Name	GR917586
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	460

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR917586 vs. SwissProt
Match: SUBL_ARATH (Subtilisin-like protease OS=Arabidopsis thaliana GN=ARA12 PE=1 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 7.737e-53
Identity = 97/146 (66.44%), Postives = 115/146 (78.77%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +KS HPEWSPAA+RSALMTTAY  YK+G  L D ATGK STPFDHG+GHV P  A NPGL+YDLT +DYLGFLCALNYT+ +I +++RR + CD  K YSVADLNYPSFAV  D +G     K+ R +T+
Sbjct:  539 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVG---AYKYTRTVTS 681

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BLAST of GR917586 vs. SwissProt
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 2.594e-24
Identity = 54/127 (42.52%), Postives = 75/127 (59.06%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAV 401
            +GTS+SCPH++G+A ++K+ +P WSPAA++SALMTTA            +A       F +GSGHV+P+ A+ PGLVYD    DY+ FLC   Y    +  +      C +G    V DLNYPSF +
Sbjct:  522 SGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPM---------NARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGL 639

[Close]

BLAST of GR917586 vs. SwissProt
Match: XSP1_ARATH (Xylem serine proteinase 1 OS=Arabidopsis thaliana GN=XSP1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.512e-19
Identity = 53/150 (35.33%), Postives = 83/150 (55.33%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITAL-ARRKFQCDA---GKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS++CPHV+G+AA++KS HP+W+PAA++SA++T+A    +   K            F +G G ++P  A +PGLVYD+    Y+ FLC   Y A+ +  L   R   C +   G  +    LNYP+  +   S   + +   RR +TN
Sbjct:  533 SGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNK---------DAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHD--SLNYPTIQLTLRSAKTSTLAVFRRRVTN 671

[Close]

BLAST of GR917586 vs. TrEMBL
Match: B9T4J8_RICCO (Xylem serine proteinase 1, putative OS=Ricinus communis GN=RCOM_0396690 PE=4 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 9.141e-63
Identity = 112/146 (76.71%), Postives = 132/146 (90.41%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +K+AHP+W+PAA+RSALMTTAY +YKNG  LQDSA+GK STPFDHG+GHVDPV+ALNPGLVYDLT DDYL FLCALNYTA+EIT+LAR++F CD+ KKYS+ DLNYPSFAV FDS+GGA+V K+ R LTN
Sbjct:  540 SGTSMSCPHVSGLAALLKAAHPDWTPAAIRSALMTTAYVSYKNGRNLQDSASGKDSTPFDHGAGHVDPVSALNPGLVYDLTADDYLSFLCALNYTAAEITSLARKRFTCDSSKKYSLNDLNYPSFAVNFDSIGGASVAKYTRTLTN 685

[Close]

BLAST of GR917586 vs. TrEMBL
Match: A9XG41_TOBAC (Subtilisin-like protease OS=Nicotiana tabacum GN=SBT1.1B PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 8.287e-56
Identity = 106/152 (69.74%), Postives = 122/152 (80.26%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVF------DSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +K AHP+WSPAA+RSALMTTAYT YKNG  LQD +TGK STPFDHG+GHVDPVAALNPGLVYDL  DDYL FLCALNYT+ +I ++ARR + C+  KKYSV DLNYPSFAVVF       S   ++ VK+ R LTN
Sbjct:  541 SGTSMSCPHVSGLAALLKGAHPDWSPAAIRSALMTTAYTVYKNGGALQDVSTGKPSTPFDHGAGHVDPVAALNPGLVYDLRADDYLNFLCALNYTSIQINSIARRNYNCETSKKYSVTDLNYPSFAVVFLEQMTAGSGSSSSSVKYTRTLTN 692

[Close]

BLAST of GR917586 vs. TrEMBL
Match: A9XG40_TOBAC (Subtilisin-like protease OS=Nicotiana tabacum GN=SBT1.1A PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 8.287e-56
Identity = 106/152 (69.74%), Postives = 122/152 (80.26%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVF------DSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +K AHP+WSPAA+RSALMTTAYT YKNG  LQD +TGK STPFDHG+GHVDPVAALNPGLVYDL  DDYL FLCALNYT+ +I ++ARR + C+  KKYSV DLNYPSFAVVF       S   ++ VK+ R LTN
Sbjct:  541 SGTSMSCPHVSGLAALLKGAHPDWSPAAIRSALMTTAYTVYKNGGALQDVSTGKPSTPFDHGAGHVDPVAALNPGLVYDLRADDYLNFLCALNYTSIQINSIARRNYNCETSKKYSVTDLNYPSFAVVFPEQMTAGSGSSSSSVKYTRTLTN 692

[Close]

BLAST of GR917586 vs. TrEMBL
Match: Q2HRK7_MEDTR (Protease-associated PA; Proteinase inhibitor I9, subtilisin propeptide OS=Medicago truncatula GN=MtrDRAFT_AC158501g3v2 PE=4 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 6.566e-53
Identity = 101/153 (66.01%), Postives = 122/153 (79.74%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSM----GGAN---VVKHRRILTN 458
            +GTS+SCPH SGLAA +K A+PEWSPAA+RSALMTTAYT+YKNG  + D ATGK +TPFD GSGHVDPV+AL+PGLVYD+ VDDYLGF CALNYT+ +I   ARR+F CDA KKY V D NYPSFAV  ++     GG+N   +V++ R+LTN
Sbjct:  535 SGTSMSCPHASGLAAIVKGAYPEWSPAAIRSALMTTAYTSYKNGQTIVDVATGKPATPFDFGSGHVDPVSALDPGLVYDINVDDYLGFFCALNYTSYQIKLAARREFTCDARKKYRVEDFNYPSFAVALETASGIGGGSNKPIIVEYNRVLTN 687

[Close]

BLAST of GR917586 vs. TrEMBL
Match: D3YBD3_TRIRP (Proteinase inhibitor OS=Trifolium repens PE=4 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.495e-52
Identity = 100/153 (65.36%), Postives = 120/153 (78.43%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGG-------ANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +K AHPEWSPAA+RSALMTT+Y AYKNG  ++D ATG  +TPFD+G+GHVDPVAAL+PGLVYD T DDYL FLCALNYT+ +I  +ARR+F CD   KY V DLNYPSFA  FD+  G       +  V+++RILTN
Sbjct:  540 SGTSMSCPHVSGLAALLKGAHPEWSPAAIRSALMTTSYRAYKNGQTIKDVATGIPATPFDYGAGHVDPVAALDPGLVYDTTADDYLSFLCALNYTSFQIKLVARREFTCDKRIKYRVEDLNYPSFAATFDAASGGKGGSHKSTTVQYKRILTN 692

[Close]

BLAST of GR917586 vs. TrEMBL
Match: D7MV52_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496864 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 4.256e-52
Identity = 98/146 (67.12%), Postives = 116/146 (79.45%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +KS HPEWSPAA+RSALMTTAY  YK+G  L D ATGK STPFDHG+GHV P  A NPGL+YDL+ +DYLGFLCALNYT+S+I +++RR + CD  K YSVADLNYPSFAV  D   GA   K+ R +T+
Sbjct:  535 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLSTEDYLGFLCALNYTSSQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVD---GAGAYKYTRTVTS 677

[Close]

BLAST of GR917586 vs. TrEMBL
Match: B9R726_RICCO (Xylem serine proteinase 1, putative OS=Ricinus communis GN=RCOM_1588040 PE=4 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.617e-51
Identity = 100/153 (65.36%), Postives = 125/153 (81.70%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSM----GGANV---VKHRRILTN 458
            +GTS+SCPHVSGLAAFIK+AH +WSPAA+RSALMTTAYTAYK+G  + D +TG+ +TPFD+G+GHV+P+AAL+PGLVYD TV+DYLGFLCALNY+A++I A+  R F CD  KKYS+ DLNYPSF+V  ++     GGA V   VK+ R LTN
Sbjct:  542 SGTSMSCPHVSGLAAFIKAAHQDWSPAAIRSALMTTAYTAYKSGKTILDVSTGQPATPFDYGAGHVNPLAALDPGLVYDATVEDYLGFLCALNYSAAQIKAVINRDFTCDPAKKYSLGDLNYPSFSVPLETASGKGGGAGVTSTVKYTRTLTN 694

[Close]

BLAST of GR917586 vs. TrEMBL
Match: B9GNR4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_551801 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 3.984e-50
Identity = 99/153 (64.71%), Postives = 119/153 (77.78%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSM----GGANV---VKHRRILTN 458
            +GTS+SCPHVSGLAA IK+AH +WSPAA++SALMTTAY  YKNG  L D ATGK STPFD+G+GHV+PVAAL+PGLVYD TVDDY+ F CALNY+AS+I  +  + F CD+ KKYS+ DLNYPSF+V   +     GGA V   VK+ R LTN
Sbjct:  552 SGTSMSCPHVSGLAALIKAAHQDWSPAAIKSALMTTAYATYKNGENLLDVATGKPSTPFDYGAGHVNPVAALDPGLVYDATVDDYISFFCALNYSASDIKQITTKDFICDSSKKYSLGDLNYPSFSVPLQTASGKEGGAGVKSTVKYTRTLTN 704

[Close]

BLAST of GR917586 vs. TrEMBL
Match: A9QMD5_PANQU (Putative uncharacterized protein (Fragment) OS=Panax quinquefolius PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 6.795e-50
Identity = 97/153 (63.40%), Postives = 118/153 (77.12%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGAN-------VVKHRRILTN 458
            +GTS+SCPH+SGLAA +KSAHPEWSPAA+RSALMTTAYT YKNG  +QD ATG  STPFD+G+GHV PVAA +PGLVY+ TV+DYL FLCALNY+++ I A+ ++ F C   KKYSV DLNYPSFAV   +  G +       +VK+ R LTN
Sbjct:   33 SGTSMSCPHLSGLAALVKSAHPEWSPAAIRSALMTTAYTTYKNGETIQDIATGMPSTPFDYGAGHVTPVAAFDPGLVYNATVEDYLDFLCALNYSSNLIKAVTKQDFTCKLDKKYSVGDLNYPSFAVHLQTASGKSGSNSTPTIVKYTRTLTN 185

[Close]

BLAST of GR917586 vs. TrEMBL
Match: Q8RVJ7_POPCA (Putative serine protease OS=Populus canadensis GN=sp PE=2 SV=2)

HSP 1 Score: 197.593 bits (501), Expect = 3.372e-49
Identity = 98/153 (64.05%), Postives = 118/153 (77.12%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSM----GGANV---VKHRRILTN 458
            +GTS+SCPHVSGLAA IK+AH +WSPAA++SALMTTAY  YKNG  L D ATG+ STPFD+G+GHV+PVAAL+PGLVYD TVDDY+ F CALNY+AS+I  +  + F CD+ KKYS  DLNYPSF+V   +     GGA V   VK+ R LTN
Sbjct:  339 SGTSMSCPHVSGLAALIKAAHQDWSPAAIKSALMTTAYATYKNGEDLLDVATGQPSTPFDYGAGHVNPVAALDPGLVYDATVDDYISFFCALNYSASDIKQITTKDFICDSSKKYSPGDLNYPSFSVPLQTASGKEGGAGVKSTVKYTRTLTN 491

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT5G67360.1 (| Symbols: ARA12 | Subtilase family protein | chr5:26872192-26874465 REVERSE LENGTH=757)

HSP 1 Score: 205.682 bits (522), Expect = 9.250e-54
Identity = 97/146 (66.44%), Postives = 115/146 (78.77%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA +KS HPEWSPAA+RSALMTTAY  YK+G  L D ATGK STPFDHG+GHV P  A NPGL+YDLT +DYLGFLCALNYT+ +I +++RR + CD  K YSVADLNYPSFAV  D +G     K+ R +T+
Sbjct:  539 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVG---AYKYTRTVTS 681

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT3G14240.1 (| Symbols: | Subtilase family protein | chr3:4741637-4743964 REVERSE LENGTH=775)

HSP 1 Score: 163.31 bits (412), Expect = 5.265e-41
Identity = 78/148 (52.70%), Postives = 105/148 (70.95%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYS-VADLNYPSFAVVFDSMGGANVVKH-RRILTN 458
            +GTS++CPHVSGLAA +K+AHP+WSPAA+RSAL+TTAYT   +G  + D +TG  S+  D+GSGHV P  A++PGLVYD+T  DY+ FLC  NYT + I  + RR+  CD  ++   V +LNYPSF+VVF   G + +  H  R +TN
Sbjct:  546 SGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTN 693

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT3G14067.1 (| Symbols: | Subtilase family protein | chr3:4658421-4660754 REVERSE LENGTH=777)

HSP 1 Score: 159.458 bits (402), Expect = 7.602e-40
Identity = 79/149 (53.02%), Postives = 103/149 (69.13%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKF---QCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSGLAA ++ AHP+WSPAA++SAL+TTAY    +G  ++D ATGK S  F HG+GHVDP  ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C+  K  +  DLNYPSF+VVF S G   VVK++R++ N
Sbjct:  543 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTG--EVVKYKRVVKN 689

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT2G05920.1 (| Symbols: | Subtilase family protein | chr2:2269831-2272207 REVERSE LENGTH=754)

HSP 1 Score: 159.458 bits (402), Expect = 7.602e-40
Identity = 77/147 (52.38%), Postives = 104/147 (70.75%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYS-VADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPH+SGLA  +K+AHPEWSP+A++SALMTTAY        L D+A    S P+ HGSGHVDP  AL+PGLVYD++ ++Y+ FLC+L+YT   I A+ +R    +  KK+S    LNYPSF+V+F   GG  VV++ R +TN
Sbjct:  533 SGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP-SVNCSKKFSDPGQLNYPSFSVLF---GGKRVVRYTREVTN 675

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT4G34980.1 (| Symbols: SLP2 | subtilisin-like serine protease 2 | chr4:16656929-16659223 REVERSE LENGTH=764)

HSP 1 Score: 145.206 bits (365), Expect = 1.484e-35
Identity = 66/129 (51.16%), Postives = 89/129 (68.99%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVF 407
            +GTS++CPHVSG AA +KSAHP+WSPA +RSA+MTT      +   L D +TGK +TP+D+GSGH++   A+NPGLVYD+T DDY+ FLC++ Y    I  + R   +C   +K S  +LNYPS   VF
Sbjct:  535 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF 663

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT5G59120.1 (| Symbols: ATSBT4.13, SBT4.13 | subtilase 4.13 | chr5:23864897-23868020 REVERSE LENGTH=732)

HSP 1 Score: 135.576 bits (340), Expect = 1.176e-32
Identity = 65/146 (44.52%), Postives = 91/146 (62.33%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHV+G+AA++K+ +P+WSP+ ++SA+MTTA+     G       TG  ST F +GSGHVDP+AA NPGLVY+L   D++ FLC +NYT+  +  ++     C   KK    +LNYPS +      G    V   R LTN
Sbjct:  515 SGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATG-------TGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTN 653

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT2G04160.1 (| Symbols: AIR3 | Subtilisin-like serine endopeptidase family protein | chr2:1401450-1407694 REVERSE LENGTH=772)

HSP 1 Score: 132.88 bits (333), Expect = 7.620e-32
Identity = 66/129 (51.16%), Postives = 88/129 (68.22%), Query Frame = 3

Query:   15 ARAGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAV 401
            A +GTS+SCPH+SG+A  +K+ +P WSPAA+RSA+MTTA         +Q+ AT   +TPF  G+GHV P  A+NPGLVYDL + DYL FLC+L Y AS+I+  +   F C +  K S+ +LNYPS  V
Sbjct:  556 AISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN-ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTC-SSPKISLVNLNYPSITV 682

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT5G59130.2 (| Symbols: | Subtilase family protein | chr5:23870192-23873691 REVERSE LENGTH=726)

HSP 1 Score: 130.568 bits (327), Expect = 3.782e-31
Identity = 61/149 (40.94%), Postives = 94/149 (63.09%), Query Frame = 3

Query:   12 AARAGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +  +GTS+SCPH +G+AA++K+ HP+WSP+ ++SA+MTTA++       +  S +G  ST F +G+GHVDP+AA NPGLVY++T  DY  FLC +NY  + +  ++     C   +K S  +LNYPS +        + +V   R +TN
Sbjct:  502 SVESGTSMSCPHAAGVAAYVKTFHPQWSPSMIKSAIMTTAWS-------MNASQSGYASTEFAYGAGHVDPIAATNPGLVYEITKTDYFAFLCGMNYNKTTVKLISGEAVTC--SEKISPRNLNYPSMSAKLSGSNISFIVTFNRTVTN 641

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BLAST of GR917586 vs. TAIR peptide
Match: AT5G59130.1 (| Symbols: | Subtilase family protein | chr5:23870192-23873691 REVERSE LENGTH=732)

HSP 1 Score: 130.568 bits (327), Expect = 3.782e-31
Identity = 61/149 (40.94%), Postives = 94/149 (63.09%), Query Frame = 3

Query:   12 AARAGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQCDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +  +GTS+SCPH +G+AA++K+ HP+WSP+ ++SA+MTTA++       +  S +G  ST F +G+GHVDP+AA NPGLVY++T  DY  FLC +NY  + +  ++     C   +K S  +LNYPS +        + +V   R +TN
Sbjct:  508 SVESGTSMSCPHAAGVAAYVKTFHPQWSPSMIKSAIMTTAWS-------MNASQSGYASTEFAYGAGHVDPIAATNPGLVYEITKTDYFAFLCGMNYNKTTVKLISGEAVTC--SEKISPRNLNYPSMSAKLSGSNISFIVTFNRTVTN 647

[Close]

BLAST of GR917586 vs. TAIR peptide
Match: AT5G51750.1 (| Symbols: ATSBT1.3, SBT1.3 | subtilase 1.3 | chr5:21020266-21022608 FORWARD LENGTH=780)

HSP 1 Score: 130.183 bits (326), Expect = 4.939e-31
Identity = 63/147 (42.86%), Postives = 92/147 (62.59%), Query Frame = 3

Query:   21 AGTSISCPHVSGLAAFIKSAHPEWSPAAVRSALMTTAYTAYKNGLKLQDSATGKYSTPFDHGSGHVDPVAALNPGLVYDLTVDDYLGFLCALNYTASEITALARRKFQ-CDAGKKYSVADLNYPSFAVVFDSMGGANVVKHRRILTN 458
            +GTS+SCPHVSG+AA IKS HP+WSPAA++SALMTTAY        L D++    S+P+DHG+GH+DP+ A +PGLVYD+   +Y  FLC  + + S++    +   + C      +  +LNYP+ + +F        +  RR +TN
Sbjct:  557 SGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTN 703

The following BLAST results are available for this feature:

BLAST of GR917586 vs. SwissProt
BLAST of GR917586 vs. TrEMBL
BLAST of GR917586 vs. TAIR peptide

BLAST of GR917586 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
SUBL_ARATH	7.737e-53	66.44	Subtilisin-like protease OS=Arabidopsis thaliana G...	[more]
CUCM1_CUCME	2.594e-24	42.52	Cucumisin OS=Cucumis melo PE=1 SV=1	[more]
XSP1_ARATH	2.512e-19	35.33	Xylem serine proteinase 1 OS=Arabidopsis thaliana ...	[more]

BLAST of GR917586 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T4J8_RICCO	9.141e-63	76.71	Xylem serine proteinase 1, putative OS=Ricinus com...	[more]
A9XG41_TOBAC	8.287e-56	69.74	Subtilisin-like protease OS=Nicotiana tabacum GN=S...	[more]
A9XG40_TOBAC	8.287e-56	69.74	Subtilisin-like protease OS=Nicotiana tabacum GN=S...	[more]
Q2HRK7_MEDTR	6.566e-53	66.01	Protease-associated PA; Proteinase inhibitor I9, s...	[more]
D3YBD3_TRIRP	2.495e-52	65.36	Proteinase inhibitor OS=Trifolium repens PE=4 SV=1	[more]
D7MV52_ARALY	4.256e-52	67.12	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9R726_RICCO	1.617e-51	65.36	Xylem serine proteinase 1, putative OS=Ricinus com...	[more]
B9GNR4_POPTR	3.984e-50	64.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9QMD5_PANQU	6.795e-50	63.40	Putative uncharacterized protein (Fragment) OS=Pan...	[more]
Q8RVJ7_POPCA	3.372e-49	64.05	Putative serine protease OS=Populus canadensis GN=...	[more]

BLAST of GR917586 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G67360.1	9.250e-54	66.44	\| Symbols: ARA12 \| Subtilase family protein \| chr5...	[more]
AT3G14240.1	5.265e-41	52.70	\| Symbols: \| Subtilase family protein \| chr3:4741...	[more]
AT3G14067.1	7.602e-40	53.02	\| Symbols: \| Subtilase family protein \| chr3:4658...	[more]
AT2G05920.1	7.602e-40	52.38	\| Symbols: \| Subtilase family protein \| chr2:2269...	[more]
AT4G34980.1	1.484e-35	51.16	\| Symbols: SLP2 \| subtilisin-like serine protease ...	[more]
AT5G59120.1	1.176e-32	44.52	\| Symbols: ATSBT4.13, SBT4.13 \| subtilase 4.13 \| c...	[more]
AT2G04160.1	7.620e-32	51.16	\| Symbols: AIR3 \| Subtilisin-like serine endopepti...	[more]
AT5G59130.2	3.782e-31	40.94	\| Symbols: \| Subtilase family protein \| chr5:2387...	[more]
AT5G59130.1	3.782e-31	40.94	\| Symbols: \| Subtilase family protein \| chr5:2387...	[more]
AT5G51750.1	4.939e-31	42.86	\| Symbols: ATSBT1.3, SBT1.3 \| subtilase 1.3 \| chr5...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	GENE3D	3.40.50.200	no description	coord: 7..91 score: 1.4
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	PFAM	PF00082	Peptidase_S8	coord: 7..61 score: 3.9
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	SUPERFAMILY	52743	Subtilisin-like	coord: 8..85 score: 2.5
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	SUBTILISIN/KEXIN-RELATED SERINE PROTEASE	coord: 8..152 score: 1.3
IPR022398	Peptidase S8/S53, subtilisin, active site	PROSITE	PS00138	SUBTILASE_SER	coord: 8..18 score
None	No IPR available	PANTHER	PTHR10795:SF17	SUBTILISIN-LIKE PROTEASE (PLANT)	coord: 8..152 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR917586 ID=GR917586; Name=GR917586; organism=Cicer arietinum; type=EST; length=460bp
GATTTCGAGCGGCCGCCCGGGCAGGTACTTCCATTTCCTGTCCTCACGTG
AGTGGTTTAGCTGCGTTCATTAAATCGGCTCATCCCGAGTGGAGTCCAGC
TGCCGTGAGATCTGCTCTTATGACAACGGCTTATACGGCTTACAAAAACG
GTCTAAAATTGCAGGACAGTGCTACTGGAAAATATTCGACGCCGTTTGAT
CATGGATCTGGACACGTGGACCCTGTTGCTGCACTTAATCCAGGACTCGT
TTATGATTTAACAGTGGATGATTATTTAGGTTTCCTATGCGCGTTAAACT
ATACAGCCTCCGAAATCACAGCTTTGGCAAGGAGGAAATTCCAGTGTGAC
GCAGGTAAAAAATACAGTGTGGCTGATCTTAATTACCCTTCGTTTGCGGT
GGTTTTTGATTCGATGGGTGGGGCCAACGTAGTCAAACACAGGAGAATTC
TTACCAACGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR015500	Peptidase_S8_subtilisin-rel
IPR022398	Peptidase_S8_His-AS

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