CK148648

EST Overview


Name	CK148648
Unique Name	CK148648
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	704

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK148648 vs. SwissProt
Match: AAK1_HUMAN (AP2-associated protein kinase 1 OS=Homo sapiens GN=AAK1 PE=1 SV=3)

HSP 1 Score: 78.5666 bits (192), Expect = 3.742e-14
Identity = 45/100 (45.00%), Postives = 66/100 (66.00%), Query Frame = 3

Query:   21 KSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWF----RVNEQLPI-DLQKS-LPDRPPE 302
            K +T K DIWALGCLL+++CYF   F GES++ + +GN+ IPD  ++S  +  LIR ML+  PD RPDI QV +     + ++ PI ++Q S +P + PE
Sbjct:  237 KIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPE 335

[Close]

BLAST of CK148648 vs. SwissProt
Match: AAK1_MOUSE (AP2-associated protein kinase 1 OS=Mus musculus GN=Aak1 PE=1 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 1.857e-13
Identity = 43/100 (43.00%), Postives = 66/100 (66.00%), Query Frame = 3

Query:   21 KSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWF----RVNEQLPI-DLQKS-LPDRPPE 302
            K +T K DIWALGCLL+++CYF   F GES++ + +G++ IPD  ++S  +  LIR ML+  PD RPDI QV +     + ++ P+ ++Q S +P + PE
Sbjct:  237 KIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGSFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPAKLPE 335

[Close]

BLAST of CK148648 vs. SwissProt
Match: BMP2K_HUMAN (BMP-2-inducible protein kinase OS=Homo sapiens GN=BMP2K PE=1 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 5.404e-13
Identity = 36/72 (50.00%), Postives = 51/72 (70.83%), Query Frame = 3

Query:   21 KSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQV 236
            K +T K DIWALGCLL+++C+F   F GES++ + +GN+ IPD  ++S  +  LIR ML+  P+ RPDI QV
Sbjct:  241 KPITTKADIWALGCLLYKLCFFTLPF-GESQVAICDGNFTIPDNSRYSRNIHCLIRFMLEPDPEHRPDIFQV 311

[Close]

BLAST of CK148648 vs. SwissProt
Match: BMP2K_MOUSE (BMP-2-inducible protein kinase OS=Mus musculus GN=Bmp2k PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.575e-12
Identity = 35/72 (48.61%), Postives = 51/72 (70.83%), Query Frame = 3

Query:   21 KSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQV 236
            K +T K DIWALGCLL+++C+F   F GES++ + +G++ IPD  ++S  +  LIR ML+  P+ RPDI QV
Sbjct:  238 KPITTKADIWALGCLLYKLCFFTLPF-GESQVAICDGSFTIPDNSRYSHNVHCLIRFMLEPDPECRPDIFQV 308

[Close]

BLAST of CK148648 vs. SwissProt
Match: AAK1_RAT (AP2-associated protein kinase 1 OS=Rattus norvegicus GN=Aak1 PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.019e-11
Identity = 42/100 (42.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:   21 KSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWF----RVNEQLPI-DLQKS-LPDRPPE 302
            K +T K DIWALGCLL+++CYF   F GES++ + +G++ IPD  ++S  +  LI  ML+  PD RPDI QV +     + ++ P+ ++Q S +P + PE
Sbjct:  237 KIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGSFTIPDNSRYSQDMHCLIY-MLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPTKLPE 334

[Close]

BLAST of CK148648 vs. SwissProt
Match: Y6461_DICDI (Probable serine/threonine-protein kinase DDB_G0276461 OS=Dictyostelium discoideum GN=DDB_G0276461 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.738e-11
Identity = 34/76 (44.74%), Postives = 50/76 (65.79%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNE 254
            + +K D+WALGCLLF++ ++   FDG S LQ++N NY+IPD  K+S+    LI+ +L A P  RP I  +   +NE
Sbjct:  248 IDEKVDVWALGCLLFKMAFYVDPFDGGS-LQIINNNYKIPDNSKYSNNFHKLIQFILVADPTQRPSINDLLNYLNE 322

[Close]

BLAST of CK148648 vs. SwissProt
Match: PRK1_YEAST (Actin-regulating kinase PRK1 OS=Saccharomyces cerevisiae GN=PRK1 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.922e-10
Identity = 38/121 (31.40%), Postives = 62/121 (51.24%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPELPLATFTKVLQCPQTNPLQCLAESTSSSF 389
            + +K DIWALG  L++ICY+ + F+   +  +L+  Y+ P  P++S  L +LIR ML   P  RP+I QV   V+          L ++P   P+  F  +    Q    Q   E +S+++
Sbjct:  222 IDEKSDIWALGVFLYKICYYTTPFEKSGEAGILHARYQYPSFPQYSDRLKNLIRLMLMEAPSQRPNICQVLEEVSR---------LQNKP--CPIRNFYLLRAMNQNANTQLAGEPSSTTY 331

[Close]

BLAST of CK148648 vs. SwissProt
Match: GAK_HUMAN (Cyclin-G-associated kinase OS=Homo sapiens GN=GAK PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 1.627e-9
Identity = 29/74 (39.19%), Postives = 46/74 (62.16%), Query Frame = 3

Query:   33 KKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNE 254
            +K DIWALGC+L+ +C+ +  F+  +KL+++NG Y IP      +    LIR MLQ  P++R  I +V  ++ E
Sbjct:  242 EKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQE 315

[Close]

BLAST of CK148648 vs. SwissProt
Match: ARK1_YEAST (Actin-regulating kinase 1 OS=Saccharomyces cerevisiae GN=ARK1 PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.627e-9
Identity = 28/75 (37.33%), Postives = 46/75 (61.33%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVN 251
            + +K DIWALG  L+++CY+ + F+    L +L+G +  P  P +S  L  LIRD+L   P  RP++ Q+  R++
Sbjct:  223 IDEKSDIWALGIFLYKLCYYTTPFEKGGDLAILSGKFEFPLYPNYSEQLKGLIRDILVQDPRHRPNVYQLLKRIS 297

[Close]

BLAST of CK148648 vs. SwissProt
Match: Y0111_DICDI (Probable serine/threonine-protein kinase DDB_G0280111 OS=Dictyostelium discoideum GN=DDB_G0280111 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.125e-9
Identity = 30/78 (38.46%), Postives = 48/78 (61.54%), Query Frame = 3

Query:   18 SKSLTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVN 251
            S  + +K DIWALGCLL+++ ++ + F+    L +LN NY IP     S+ L  LI+ ML   P +RP+I ++  ++N
Sbjct:  209 SPVINEKIDIWALGCLLYKLLFYVTPFEDSGSLGILNSNYTIPPNHTHSNDLISLIKIMLNPDPINRPNIFEITNQLN 286

[Close]

BLAST of CK148648 vs. TrEMBL
Match: B9REI2_RICCO (Serine/threonine protein kinase, putative OS=Ricinus communis GN=RCOM_1771100 PE=4 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 2.326e-49
Identity = 80/109 (73.39%), Postives = 92/109 (84.40%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPELPLATFTKVLQCPQTNP 353
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIPDLPK+S  +TDLIRDMLQA PDDRPDITQVWFRVNEQLP++LQKSLPD  PE+   +  +V   P   P
Sbjct:  220 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPDLPKYSMPVTDLIRDMLQASPDDRPDITQVWFRVNEQLPVNLQKSLPDGLPEMKSTSVHEVTSRPANRP 328

HSP 2 Score: 50.447 bits (119), Expect = 2.326e-49
Identity = 34/90 (37.78%), Postives = 44/90 (48.89%), Query Frame = 1

Query:  412 PQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEEKGKPIYDEERS------------SHHVSLKHDRVRPDNDQLPKNASSNKGAQHTNSD 645
            P PS    GG  GGQLGAFWS+ HA+DS V EEK K  +D+E S            +H +S  +  V+ +N Q         G  H + D
Sbjct:  338 PPPSSGGVGG--GGQLGAFWSSQHARDSFV-EEKSKSKFDDESSYNPSRQDKSQSENHTLSKTYSPVKDENSQAHATRRIVHGKSHVSQD 424

[Close]

BLAST of CK148648 vs. TrEMBL
Match: D7LFM3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482227 PE=4 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 5.348e-42
Identity = 74/93 (79.57%), Postives = 89/93 (95.70%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S+F+TDLI++MLQA PD+RPDITQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSAFVTDLIKEMLQASPDERPDITQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 28.1054 bits (61), Expect = 5.348e-42
Identity = 11/21 (52.38%), Postives = 15/21 (71.43%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S NKS  +PRR
Sbjct:  315 STGVHDGSSKSANKSSPVPRR 335

[Close]

BLAST of CK148648 vs. TrEMBL
Match: Q8H0Z0_ARATH (Putative serine/threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 2.833e-40
Identity = 74/93 (79.57%), Postives = 88/93 (94.62%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 23.0978 bits (48), Expect = 2.833e-40
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S  K    PRR
Sbjct:  315 STGVHDGSSKSATKPSPAPRR 335

[Close]

BLAST of CK148648 vs. TrEMBL
Match: O50071_ARATH (Putative serine threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=2)

HSP 1 Score: 167.548 bits (423), Expect = 2.834e-40
Identity = 74/93 (79.57%), Postives = 88/93 (94.62%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 23.0978 bits (48), Expect = 2.834e-40
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S  K    PRR
Sbjct:  315 STGVHDGSSKSATKPSPAPRR 335

[Close]

BLAST of CK148648 vs. TrEMBL
Match: Q8VYC0_ARATH (Putative serine/threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 3.693e-40
Identity = 73/93 (78.49%), Postives = 88/93 (94.62%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPD+TQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDVTQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 23.0978 bits (48), Expect = 3.693e-40
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S  K    PRR
Sbjct:  315 STGVHDGSSKSATKPSPAPRR 335

[Close]

BLAST of CK148648 vs. TrEMBL
Match: Q69L76_ORYSJ (Cyclin G-associated kinase-like protein OS=Oryza sativa subsp. japonica GN=OSJNBa0016G10.6-1 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.491e-34
Identity = 66/88 (75.00%), Postives = 82/88 (93.18%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPD 290
            +++K DIWALGCLL+RICYFKSAFDGESKLQ+LNGNYRIP+ PK+S+ +T LI+DML+A P+DRPD+TQVWFRVNE LP++LQK+LPD
Sbjct:  220 ISEKVDIWALGCLLYRICYFKSAFDGESKLQILNGNYRIPEQPKYSAAVTKLIKDMLEASPNDRPDVTQVWFRVNELLPLELQKNLPD 307

[Close]

BLAST of CK148648 vs. TrEMBL
Match: B8AEP9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_07872 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.491e-34
Identity = 66/88 (75.00%), Postives = 82/88 (93.18%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPD 290
            +++K DIWALGCLL+RICYFKSAFDGESKLQ+LNGNYRIP+ PK+S+ +T LI+DML+A P+DRPD+TQVWFRVNE LP++LQK+LPD
Sbjct:  220 ISEKVDIWALGCLLYRICYFKSAFDGESKLQILNGNYRIPEQPKYSAAVTKLIKDMLEASPNDRPDVTQVWFRVNELLPLELQKNLPD 307

[Close]

BLAST of CK148648 vs. TrEMBL
Match: B4FZT6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.381e-32
Identity = 66/90 (73.33%), Postives = 78/90 (86.67%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRP 296
            +++K DIWALGCLL+RICYFKSAFDGESKLQVLNGNY IP+ PK+S  +T LI+DML+A P+ RPDITQVWFRVNE L ++LQK LPD P
Sbjct:  103 ISEKVDIWALGCLLYRICYFKSAFDGESKLQVLNGNYHIPEQPKYSIAVTGLIKDMLEASPNARPDITQVWFRVNELLSLELQKRLPDGP 192

[Close]

BLAST of CK148648 vs. TrEMBL
Match: Q6H5H4_ORYSJ (Putative AAK1 protein OS=Oryza sativa subsp. japonica GN=P0701E06.30 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.886e-29
Identity = 63/91 (69.23%), Postives = 75/91 (82.42%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPP 299
            +++K DIWALGCLL+RICY KSAFDGESKLQ+LNGNYRIP+LPK+SS +T LI+DMLQ+ PD       VWFRVNE LP++LQK LPD  P
Sbjct:  222 ISEKVDIWALGCLLYRICYLKSAFDGESKLQILNGNYRIPELPKYSSPITSLIKDMLQSSPD-------VWFRVNELLPLELQKDLPDGSP 305

[Close]

BLAST of CK148648 vs. TrEMBL
Match: B9G2I7_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_28650 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.886e-29
Identity = 63/91 (69.23%), Postives = 75/91 (82.42%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPP 299
            +++K DIWALGCLL+RICY KSAFDGESKLQ+LNGNYRIP+LPK+SS +T LI+DMLQ+ PD       VWFRVNE LP++LQK LPD  P
Sbjct:  222 ISEKVDIWALGCLLYRICYLKSAFDGESKLQILNGNYRIPELPKYSSPITSLIKDMLQSSPD-------VWFRVNELLPLELQKDLPDGSP 305

[Close]

BLAST of CK148648 vs. TAIR peptide
Match: AT2G32850.2 (| Symbols: | Protein kinase superfamily protein | chr2:13935448-13937977 REVERSE LENGTH=670)

HSP 1 Score: 167.548 bits (423), Expect = 1.925e-42
Identity = 74/93 (79.57%), Postives = 88/93 (94.62%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 23.0978 bits (48), Expect = 1.925e-42
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S  K    PRR
Sbjct:  315 STGVHDGSSKSATKPSPAPRR 335

HSP 3 Score: 57.7658 bits (138), Expect = 6.865e-9
Identity = 41/109 (37.61%), Postives = 55/109 (50.46%), Query Frame = 1

Query:  334 NVHKQIPCNASQNXXXXXSVGESKTTPQPSLAPRGG-----------ESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSSHHVSLKHDRVRPDNDQLPKNASSNKG 624
            N+ K +P    +        G SK+  +PS APR             +SGG LGAFW+T HAK S V E+ K  P +DE  S+   + K +RVR D+ Q PK  S  +G
Sbjct:  301 NLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGESDSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN---TSKSERVRVDSHQ-PKKPSPVRG 405

[Close]

BLAST of CK148648 vs. TAIR peptide
Match: AT2G32850.1 (| Symbols: | Protein kinase superfamily protein | chr2:13935448-13937977 REVERSE LENGTH=650)

HSP 1 Score: 167.548 bits (423), Expect = 1.925e-42
Identity = 74/93 (79.57%), Postives = 88/93 (94.62%), Query Frame = 3

Query:   27 LTKKWDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQVWFRVNEQLPIDLQKSLPDRPPEL 305
            +++K DIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ+WFRVNEQLP +LQKSLPDRPPE+
Sbjct:  221 ISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPANLQKSLPDRPPEM 313

HSP 2 Score: 23.0978 bits (48), Expect = 1.925e-42
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 2

Query:  308 SCNIHEGASMSTNKSPAMPRR 370
            S  +H+G+S S  K    PRR
Sbjct:  315 STGVHDGSSKSATKPSPAPRR 335

HSP 3 Score: 57.7658 bits (138), Expect = 6.865e-9
Identity = 41/109 (37.61%), Postives = 55/109 (50.46%), Query Frame = 1

Query:  334 NVHKQIPCNASQNXXXXXSVGESKTTPQPSLAPRGG-----------ESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSSHHVSLKHDRVRPDNDQLPKNASSNKG 624
            N+ K +P    +        G SK+  +PS APR             +SGG LGAFW+T HAK S V E+ K  P +DE  S+   + K +RVR D+ Q PK  S  +G
Sbjct:  301 NLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGESDSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN---TSKSERVRVDSHQ-PKKPSPVRG 405

The following BLAST results are available for this feature:

BLAST of CK148648 vs. SwissProt
BLAST of CK148648 vs. TrEMBL
BLAST of CK148648 vs. TAIR peptide

BLAST of CK148648 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AAK1_HUMAN	3.742e-14	45.00	AP2-associated protein kinase 1 OS=Homo sapiens GN...	[more]
AAK1_MOUSE	1.857e-13	43.00	AP2-associated protein kinase 1 OS=Mus musculus GN...	[more]
BMP2K_HUMAN	5.404e-13	50.00	BMP-2-inducible protein kinase OS=Homo sapiens GN=...	[more]
BMP2K_MOUSE	4.575e-12	48.61	BMP-2-inducible protein kinase OS=Mus musculus GN=...	[more]
AAK1_RAT	1.019e-11	42.00	AP2-associated protein kinase 1 OS=Rattus norvegic...	[more]
Y6461_DICDI	1.738e-11	44.74	Probable serine/threonine-protein kinase DDB_G0276...	[more]
PRK1_YEAST	1.922e-10	31.40	Actin-regulating kinase PRK1 OS=Saccharomyces cere...	[more]
GAK_HUMAN	1.627e-9	39.19	Cyclin-G-associated kinase OS=Homo sapiens GN=GAK ...	[more]
ARK1_YEAST	1.627e-9	37.33	Actin-regulating kinase 1 OS=Saccharomyces cerevis...	[more]
Y0111_DICDI	2.125e-9	38.46	Probable serine/threonine-protein kinase DDB_G0280...	[more]

BLAST of CK148648 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9REI2_RICCO	2.326e-49	73.39	Serine/threonine protein kinase, putative OS=Ricin...	[more]
D7LFM3_ARALY	5.348e-42	79.57	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q8H0Z0_ARATH	2.833e-40	79.57	Putative serine/threonine protein kinase OS=Arabid...	[more]
O50071_ARATH	2.834e-40	79.57	Putative serine threonine protein kinase OS=Arabid...	[more]
Q8VYC0_ARATH	3.693e-40	78.49	Putative serine/threonine protein kinase OS=Arabid...	[more]
Q69L76_ORYSJ	1.491e-34	75.00	Cyclin G-associated kinase-like protein OS=Oryza s...	[more]
B8AEP9_ORYSI	1.491e-34	75.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FZT6_MAIZE	2.381e-32	73.33	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q6H5H4_ORYSJ	1.886e-29	69.23	Putative AAK1 protein OS=Oryza sativa subsp. japon...	[more]
B9G2I7_ORYSJ	1.886e-29	69.23	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of CK148648 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G32850.2	1.925e-42	79.57	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT2G32850.1	1.925e-42	79.57	\| Symbols: \| Protein kinase superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 64..68 score
None	No IPR available	SEG	seg	seg	coord: 26..48 score
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 6..88 score: 7.1
None	No IPR available	PANTHER	PTHR22967	SERINE/THREONINE PROTEIN KINASE	coord: 12..100 score: 3.7
None	No IPR available	PANTHER	PTHR22967:SF29	SERINE/THREONINE KINASE 16 (STK16 )	coord: 12..100 score: 3.7
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..86 score: 10
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 7..142 score: 2.1
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 6..78 score: 5.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148648 ID=CK148648; Name=CK148648; organism=Cicer arietinum; type=EST; length=704bp
GGGCATCTGTTCCTGAGAGTAAGTCATTAACGAAAAAGTGGGACATATGG
GCACTTGGGTGTCTCCTTTTTCGCATATGCTACTTCAAAAGTGCATTTGA
TGGGGAATCGAAGCTCCAAGTCTTAAATGGAAACTACCGCATTCCTGATT
TACCTAAGTTCAGTTCATTCCTCACAGACTTGATCAGAGACATGCTTCAA
GCTAGACCAGATGACAGACCAGATATCACGCAGGTTTGGTTTCGTGTTAA
TGAGCAGCTACCCATTGATTTACAAAAGTCTTTACCTGACAGGCCACCTG
AATTGCCTCTTGCAACATTCACGAAGGTGCTTCAATGTCCACAAACAAAT
CCCCTGCAATGCCTCGCAGAATCCACCTCCTCCTCCTTCAGTTGGAGAAT
CCAAAACTACCCCTCAGCCATCTCTTGCTCCTAGGGGAGGGGAAAGTGGG
GGGCAGCTTGGTGCTTTCTGGTCCACTCTGCATGCAAAGGATTCACCTGT
CCCCGAGGAAAAGGGAAAACCTATATATGATGAAGAACGATCTAGCCACC
ATGTATCATTGAAACATGACAGAGTTCGTCCAGACAATGATCAGTTACCC
AAAAATGCTAGTTCTAACAAAGGGGCCCAACACACAAACTCAGACTGTAA
AAAGCAATATGCATGGAAAATTACCGAAGCCTGCTGCCGTATCACCAGGA
CTTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain

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