DY475547

EST Overview
NameDY475547
Unique NameDY475547
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length255
Libraries
Library NameType
LIBEST_016683 Chickpea (ICC3996) post-inoculation EST librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of DY475547 vs. TrEMBL
Match: Q652S1_ORYSJ (Os06g0258900 protein OS=Oryza sativa subsp. japonica GN=P0603C10.39-1 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.044e-22
Identity = 29/41 (70.73%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +PLWQL P  RHPGVPYIVFP NV D   LA+VV+
Sbjct: 1051 GQALAGVPLWQLGPESRHPGVPYIVFPGNVGDNSALAKVVQ 1091

HSP 2 Score: 64.6994 bits (156), Expect = 2.044e-22
Identity = 30/43 (69.77%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  125 EXWTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            + W CP+R SS KE+L NAENGGYA+GAFNVYN+EG  AVV A
Sbjct: 1091 QNWACPSR-SSAKELLLNAENGGYAIGAFNVYNLEGIDAVVSA 1132
[Close]
BLAST of DY475547 vs. TrEMBL
Match: B8B0B8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_22435 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.044e-22
Identity = 29/41 (70.73%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +PLWQL P  RHPGVPYIVFP NV D   LA+VV+
Sbjct: 1051 GQALAGVPLWQLGPESRHPGVPYIVFPGNVGDNSALAKVVQ 1091

HSP 2 Score: 64.6994 bits (156), Expect = 2.044e-22
Identity = 30/43 (69.77%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  125 EXWTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            + W CP+R SS KE+L NAENGGYA+GAFNVYN+EG  AVV A
Sbjct: 1091 QNWACPSR-SSAKELLLNAENGGYAIGAFNVYNLEGIDAVVSA 1132
[Close]
BLAST of DY475547 vs. TrEMBL
Match: Q652S0_ORYSJ (Ketose-bisphosphate aldolase class-II family-like OS=Oryza sativa subsp. japonica GN=P0603C10.39-2 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.089e-22
Identity = 29/41 (70.73%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +PLWQL P  RHPGVPYIVFP NV D   LA+VV+
Sbjct:  185 GQALAGVPLWQLGPESRHPGVPYIVFPGNVGDNSALAKVVQ 225

HSP 2 Score: 64.6994 bits (156), Expect = 2.089e-22
Identity = 30/43 (69.77%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  125 EXWTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            + W CP+R SS KE+L NAENGGYA+GAFNVYN+EG  AVV A
Sbjct:  225 QNWACPSR-SSAKELLLNAENGGYAIGAFNVYNLEGIDAVVSA 266
[Close]
BLAST of DY475547 vs. TrEMBL
Match: D7SJD5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008461001 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 5.850e-22
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +PLWQL P  RHPGVPYIVFP NV D + LA+VVK
Sbjct: 1183 GQALAGVPLWQLGPESRHPGVPYIVFPGNVGDSKALADVVK 1223

HSP 2 Score: 60.8474 bits (146), Expect = 5.850e-22
Identity = 29/41 (70.73%), Postives = 33/41 (80.49%), Query Frame = 2 
Query:  131 WTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            W  P RLSSTK +L +AE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1225 WVRPFRLSSTKGLLLDAERGGYAVGAFNVYNLEGVEAVVAA 1265
[Close]
BLAST of DY475547 vs. TrEMBL
Match: C5Z818_SORBI (Putative uncharacterized protein Sb10g009360 OS=Sorghum bicolor GN=Sb10g009360 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 8.135e-21
Identity = 29/41 (70.73%), Postives = 31/41 (75.61%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +PLWQL P  R PGVPYIVFP NV D   LA+VVK
Sbjct: 1054 GQALAGVPLWQLGPESRFPGVPYIVFPGNVGDNSALAKVVK 1094

HSP 2 Score: 61.2326 bits (147), Expect = 8.135e-21
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 2 
Query:  131 WTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            W  P+R SSTKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1096 WASPSR-SSTKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1135
[Close]
BLAST of DY475547 vs. TrEMBL
Match: Q9LM23_ARATH (T10O22.24 OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.191e-19
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1161 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1201

HSP 2 Score: 55.4546 bits (132), Expect = 4.191e-19
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1210 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1242
[Close]
BLAST of DY475547 vs. TrEMBL
Match: D7KGC3_ARALY (Ketose-bisphosphate aldolase class-II family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472044 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.196e-19
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1051 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1091

HSP 2 Score: 55.4546 bits (132), Expect = 4.196e-19
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1100 STKELLLNAEKGGYAVGAFNVYNLEGVEAVVAA 1132
[Close]
BLAST of DY475547 vs. TrEMBL
Match: B3H739_ARATH (Uncharacterized protein At1g18270.2 OS=Arabidopsis thaliana GN=At1g18270 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.196e-19
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1049 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1089

HSP 2 Score: 55.4546 bits (132), Expect = 4.196e-19
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1098 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1130
[Close]
BLAST of DY475547 vs. TrEMBL
Match: Q8VYC5_ARATH (Putative uncharacterized protein At1g18270 OS=Arabidopsis thaliana GN=At1g18270 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.196e-19
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1048 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1088

HSP 2 Score: 55.4546 bits (132), Expect = 4.196e-19
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1097 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1129
[Close]
BLAST of DY475547 vs. TrEMBL
Match: A9SYE4_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_189760 PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.034e-16
Identity = 24/41 (58.54%), Postives = 30/41 (73.17%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQAL  +PLW+L P  RHP +PYIVFP NV   + LA++VK
Sbjct:  867 GQALPGVPLWKLGPGSRHPNIPYIVFPGNVGGPDALAQLVK 907

HSP 2 Score: 50.447 bits (119), Expect = 5.034e-16
Identity = 26/41 (63.41%), Postives = 30/41 (73.17%), Query Frame = 2 
Query:  131 WTCPTRLSSTKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            W+ P R  +TK+IL  AE G YAVGAFNVYN+EG  AVV A
Sbjct:  909 WSRPGR-PTTKDILQAAEEGRYAVGAFNVYNLEGIMAVVAA 948
[Close]
BLAST of DY475547 vs. TAIR peptide
Match: AT1G18270.3 (| Symbols: | ketose-bisphosphate aldolase class-II family protein | chr1:6283634-6293772 REVERSE LENGTH=1393)

HSP 1 Score: 62.7734 bits (151), Expect = 1.631e-21
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1068 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1108

HSP 2 Score: 55.4546 bits (132), Expect = 1.631e-21
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1117 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1149
[Close]
BLAST of DY475547 vs. TAIR peptide
Match: AT1G18270.2 (| Symbols: | ketose-bisphosphate aldolase class-II family protein | chr1:6283634-6293772 REVERSE LENGTH=1374)

HSP 1 Score: 62.7734 bits (151), Expect = 1.632e-21
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1049 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1089

HSP 2 Score: 55.4546 bits (132), Expect = 1.632e-21
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1098 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1130
[Close]
BLAST of DY475547 vs. TAIR peptide
Match: AT1G18270.1 (| Symbols: | ketose-bisphosphate aldolase class-II family protein | chr1:6283634-6293772 REVERSE LENGTH=1373)

HSP 1 Score: 62.7734 bits (151), Expect = 1.632e-21
Identity = 28/41 (68.29%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:    6 GQALAXIPLWQLXPXGRHPGVPYIVFPXNVXDXEPLAEVVK 128
            GQALA +P+W+L P  RHPGVPYIVFP NV +   LAEVVK
Sbjct: 1048 GQALAGVPVWKLGPESRHPGVPYIVFPGNVGNSTALAEVVK 1088

HSP 2 Score: 55.4546 bits (132), Expect = 1.632e-21
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  155 STKEILNNAENGGYAVGAFNVYNMEGXQAVVPA 253
            STKE+L NAE GGYAVGAFNVYN+EG +AVV A
Sbjct: 1097 STKELLLNAEKGGYAVGAFNVYNLEGIEAVVAA 1129
The following BLAST results are available for this feature:
BLAST of DY475547 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q652S1_ORYSJ2.044e-2270.73Os06g0258900 protein OS=Oryza sativa subsp. japoni... [more]
B8B0B8_ORYSI2.044e-2270.73Putative uncharacterized protein OS=Oryza sativa s... [more]
Q652S0_ORYSJ2.089e-2270.73Ketose-bisphosphate aldolase class-II family-like ... [more]
D7SJD5_VITVI5.850e-2273.17Whole genome shotgun sequence of line PN40024, sca... [more]
C5Z818_SORBI8.135e-2170.73Putative uncharacterized protein Sb10g009360 OS=So... [more]
Q9LM23_ARATH4.191e-1968.29T10O22.24 OS=Arabidopsis thaliana PE=4 SV=1[more]
D7KGC3_ARALY4.196e-1968.29Ketose-bisphosphate aldolase class-II family prote... [more]
B3H739_ARATH4.196e-1968.29Uncharacterized protein At1g18270.2 OS=Arabidopsis... [more]
Q8VYC5_ARATH4.196e-1968.29Putative uncharacterized protein At1g18270 OS=Arab... [more]
A9SYE4_PHYPA5.034e-1658.54Predicted protein OS=Physcomitrella patens subsp. ... [more]
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BLAST of DY475547 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT1G18270.31.631e-2168.29| Symbols: | ketose-bisphosphate aldolase class-I... [more]
AT1G18270.21.632e-2168.29| Symbols: | ketose-bisphosphate aldolase class-I... [more]
AT1G18270.11.632e-2168.29| Symbols: | ketose-bisphosphate aldolase class-I... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000771Ketose-bisphosphate aldolase, class-IIPFAMPF01116F_bP_aldolasecoord: 20..53
score: 4.7
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70no descriptioncoord: 20..53
score: 1.1
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 19..54
score: 4.7

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>DY475547 ID=DY475547; Name=DY475547; organism=Cicer arietinum; type=EST; length=255bp
GCGGGGGACAAGCACTAGCCNGAATTCCCTTGTGGCAACTANGCCCTGNG
GGNAGACATCCTGGAGTTCCTTACATTGTCTTCCCANGTAATGTCGNNGA
CANCGAACCATTGGCNGAAGTGGTGAAGNCTGGACTTGTCCAACNAGACT
TTCNTCGACGAAAGAGATTCTTAATAACGCAGAAAATGGTGGATATGCTG
TTGGGGCATTTAACGTTTATAATATGGAAGGAGNTCAAGCTGTTGTTCCT
GCTGC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004332fructose-bisphosphate aldolase activity
GO:0008270zinc ion binding
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR000771FBA_II
IPR013785Aldolase_TIM