EH059126

EST Overview


Name	EH059126
Unique Name	EH059126
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	550

Libraries


Library Name	Type
LIBEST_020668 subtracted cDNA library of drought stressed chickpea	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYM_SOLTU (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.262e-17
Identity = 40/69 (57.97%), Postives = 44/69 (63.77%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIFYR           E   YD+EDKIN AVFP L GGPHNH I  LAVALKQATTP ++AY +Q
Sbjct:  292 PRGAMIFYRKGVKEVNKQGKE-VFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQ 359

HSP 2 Score: 31.5722 bits (70), Expect = 2.262e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  279 DVVTTTTHKSLRGPR 293

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYM_PEA (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.457e-17
Identity = 39/69 (56.52%), Postives = 44/69 (63.77%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           E   YD+EDKIN AVFP L GGPHNH I  LAVALKQATTP ++AY +Q
Sbjct:  292 PRGAMIFFRKGLKEVNKQGKE-VFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQ 359

HSP 2 Score: 31.5722 bits (70), Expect = 6.457e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  279 DVVTTTTHKSLRGPR 293

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.457e-17
Identity = 40/69 (57.97%), Postives = 44/69 (63.77%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           E  +YDFEDKIN AVFP L GGPHNH I  LAVALKQATT  +KAY +Q
Sbjct:  291 PRGAMIFFRKGVKEINKQGKE-VLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQ 358

HSP 2 Score: 31.5722 bits (70), Expect = 6.457e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  278 DVVTTTTHKSLRGPR 292

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYC_NEUCR (Serine hydroxymethyltransferase, cytosolic OS=Neurospora crassa GN=for PE=3 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 1.093e-16
Identity = 36/69 (52.17%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           +  +YD EDKINF+VFP   GGPHNH I ALAVALKQA +P FK Y ++
Sbjct:  254 PRGAMIFFRRGVRSVDAKTGKETLYDLEDKINFSVFPGHQGGPHNHTITALAVALKQAASPEFKEYQQK 322

HSP 2 Score: 31.5722 bits (70), Expect = 1.093e-16
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  241 DVVTTTTHKSLRGPR 255

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.418e-16
Identity = 39/69 (56.52%), Postives = 44/69 (63.77%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           E  +YD+EDKIN AVFP L GGPHNH I  LAVALKQATT  +KAY +Q
Sbjct:  292 PRGAMIFFRKGVKEVNKQGKE-VLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQ 359

HSP 2 Score: 31.5722 bits (70), Expect = 1.418e-16
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  279 DVVTTTTHKSLRGPR 293

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYM_FLAPR (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.395e-16
Identity = 39/69 (56.52%), Postives = 43/69 (62.32%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           E   YD+EDKIN AVFP L GGPHNH I  LAVALKQATT  +KAY +Q
Sbjct:  292 PRGAMIFFRKGLKEVNKQGKE-VFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQ 359

HSP 2 Score: 31.5722 bits (70), Expect = 2.395e-16
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  279 DVVTTTTHKSLRGPR 293

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYC_CANAL (Serine hydroxymethyltransferase, cytosolic OS=Candida albicans GN=SHM2 PE=1 SV=3)

HSP 1 Score: 73.1738 bits (178), Expect = 3.119e-16
Identity = 35/69 (50.72%), Postives = 41/69 (59.42%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           +  +YD E+ INF+VFP   GGPHNH I ALA ALKQA TP FK Y +Q
Sbjct:  254 PRGAMIFFRRGVRSVNPKTGQEILYDLENPINFSVFPGHQGGPHNHTIAALATALKQANTPEFKEYQEQ 322

HSP 2 Score: 31.9574 bits (71), Expect = 3.119e-16
Identity = 14/15 (93.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            DIVTTTTHKSLR PR
Sbjct:  241 DIVTTTTHKSLRGPR 255

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYC_ASHGO (Serine hydroxymethyltransferase, cytosolic OS=Ashbya gossypii GN=SHM2 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.054e-16
Identity = 35/66 (53.03%), Postives = 39/66 (59.09%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAY 243
            P   MIF+R           E  +YD E  INF+VFP   GGPHNH I ALA ALKQATTP F+ Y
Sbjct:  253 PRGAMIFFRRGVRSVHPKTGEEVMYDLEGPINFSVFPGHQGGPHNHTISALATALKQATTPEFREY 318

HSP 2 Score: 31.9574 bits (71), Expect = 4.054e-16
Identity = 14/15 (93.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            DIVTTTTHKSLR PR
Sbjct:  240 DIVTTTTHKSLRGPR 254

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYC_YEAST (Serine hydroxymethyltransferase, cytosolic OS=Saccharomyces cerevisiae GN=SHM2 PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 5.269e-16
Identity = 35/69 (50.72%), Postives = 40/69 (57.97%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           +  +YD E+ INF+VFP   GGPHNH I ALA ALKQA TP FK Y  Q
Sbjct:  253 PRGAMIFFRRGVRSINPKTGKEVLYDLENPINFSVFPGHQGGPHNHTIAALATALKQAATPEFKEYQTQ 321

HSP 2 Score: 31.9574 bits (71), Expect = 5.269e-16
Identity = 14/15 (93.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            DIVTTTTHKSLR PR
Sbjct:  240 DIVTTTTHKSLRGPR 254

[Close]

BLAST of EH059126 vs. SwissProt
Match: GLYM_KLULA (Serine hydroxymethyltransferase, mitochondrial OS=Kluyveromyces lactis GN=SHM1 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.949e-15
Identity = 36/66 (54.55%), Postives = 40/66 (60.61%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAY 243
            P   MIFYR           E  +YD + +INF+VFP   GGPHNH I ALAVALKQA TP FK Y
Sbjct:  278 PRGAMIFYRKGVRKVTKKGKE-VLYDLDKRINFSVFPGHQGGPHNHTISALAVALKQAATPEFKEY 342

HSP 2 Score: 31.9574 bits (71), Expect = 1.949e-15
Identity = 14/15 (93.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            DIVTTTTHKSLR PR
Sbjct:  265 DIVTTTTHKSLRGPR 279

[Close]

BLAST of EH059126 vs. TrEMBL
Match: Q9FPJ3_ARATH (Serine hydroxymethyltransferase OS=Arabidopsis thaliana GN=AT4g13930/dl3005c PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.593e-26
Identity = 52/69 (75.36%), Postives = 53/69 (76.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYDFEDKINFAVFP+L GGPHNHQIGALAVALKQA TPGFK YAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 4.593e-26
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TrEMBL
Match: Q8LBY1_ARATH (Serine hydroxymethyltransferase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.593e-26
Identity = 52/69 (75.36%), Postives = 53/69 (76.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYDFEDKINFAVFP+L GGPHNHQIGALAVALKQA TPGFK YAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 4.593e-26
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TrEMBL
Match: O23254_ARATH (Serine hydroxymethyltransferase OS=Arabidopsis thaliana GN=dl3005c PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.593e-26
Identity = 52/69 (75.36%), Postives = 53/69 (76.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYDFEDKINFAVFP+L GGPHNHQIGALAVALKQA TPGFK YAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 4.593e-26
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TrEMBL
Match: D7MHA0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_915517 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.593e-26
Identity = 52/69 (75.36%), Postives = 53/69 (76.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYDFEDKINFAVFP+L GGPHNHQIGALAVALKQA TPGFK YAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 4.593e-26
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TrEMBL
Match: Q0ISV6_ORYSJ (Os11g0455800 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os11g0455800 PE=4 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 8.336e-25
Identity = 50/69 (72.46%), Postives = 52/69 (75.36%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYD+EDKINFAVFPSL GGPHNHQI ALAVAL+Q  TPGFKAYAKQ
Sbjct:  275 PRAGMIFYRKGPKPPKKGQPEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQQTMTPGFKAYAKQ 343

HSP 2 Score: 35.4242 bits (80), Expect = 8.336e-25
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  261 CDVVTTTTHKSLRGPRA 277

[Close]

BLAST of EH059126 vs. TrEMBL
Match: C5Y297_SORBI (Serine hydroxymethyltransferase OS=Sorghum bicolor GN=Sb05g014880 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 8.344e-25
Identity = 50/69 (72.46%), Postives = 52/69 (75.36%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYD+EDKINFAVFPSL GGPHNHQI ALAVAL+Q  TPGFKAYAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQQTMTPGFKAYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 8.344e-25
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TrEMBL
Match: A3CB05_ORYSJ (Serine hydroxymethyltransferase OS=Oryza sativa subsp. japonica GN=OsJ_33805 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 8.352e-25
Identity = 50/69 (72.46%), Postives = 52/69 (75.36%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYD+EDKINFAVFPSL GGPHNHQI ALAVAL+Q  TPGFKAYAKQ
Sbjct:  225 PRAGMIFYRKGPKPPKKGQPEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQQTMTPGFKAYAKQ 293

HSP 2 Score: 35.4242 bits (80), Expect = 8.352e-25
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  211 CDVVTTTTHKSLRGPRA 227

[Close]

BLAST of EH059126 vs. TrEMBL
Match: A2ZDY5_ORYSI (Serine hydroxymethyltransferase OS=Oryza sativa subsp. indica GN=OsI_35999 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 8.392e-25
Identity = 50/69 (72.46%), Postives = 52/69 (75.36%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYD+EDKINFAVFPSL GGPHNHQI ALAVAL+Q  TPGFKAYAKQ
Sbjct:  125 PRAGMIFYRKGPKPPKKGQPEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQQTMTPGFKAYAKQ 193

HSP 2 Score: 35.4242 bits (80), Expect = 8.392e-25
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  111 CDVVTTTTHKSLRGPRA 127

[Close]

BLAST of EH059126 vs. TrEMBL
Match: Q58A18_CUCME (Putative uncharacterized protein 6F11 (Fragment) OS=Cucumis melo GN=6F11 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 8.406e-25
Identity = 50/69 (72.46%), Postives = 55/69 (79.71%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E+AVYD+EDKINF+VFP+L GGPHNHQIGALAVALKQA +PGFKAYAKQ
Sbjct:  101 PRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKAYAKQ 169

HSP 2 Score: 33.4982 bits (75), Expect = 8.406e-25
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VT TTHKSLR PR+
Sbjct:   87 CDVVTATTHKSLRGPRA 103

[Close]

BLAST of EH059126 vs. TrEMBL
Match: Q2R4Y7_ORYSJ (Serine hydroxymethyltransferase, mitochondrial, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os11g26860 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 8.450e-25
Identity = 50/69 (72.46%), Postives = 52/69 (75.36%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYD+EDKINFAVFPSL GGPHNHQI ALAVAL+Q  TPGFKAYAKQ
Sbjct:   34 PRAGMIFYRKGPKPPKKGQPEGAVYDYEDKINFAVFPSLQGGPHNHQIAALAVALQQTMTPGFKAYAKQ 102

HSP 2 Score: 35.4242 bits (80), Expect = 8.450e-25
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:   20 CDVVTTTTHKSLRGPRA 36

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT4G13930.1 (| Symbols: SHM4 | serine hydroxymethyltransferase 4 | chr4:8048013-8050021 REVERSE LENGTH=471)

HSP 1 Score: 106.686 bits (265), Expect = 3.646e-28
Identity = 52/69 (75.36%), Postives = 53/69 (76.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P AGMIFYR           E AVYDFEDKINFAVFP+L GGPHNHQIGALAVALKQA TPGFK YAKQ
Sbjct:  249 PRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQANTPGFKVYAKQ 317

HSP 2 Score: 35.4242 bits (80), Expect = 3.646e-28
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPRS 53
            CD+VTTTTHKSLR PR+
Sbjct:  235 CDVVTTTTHKSLRGPRA 251

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT1G22020.1 (| Symbols: SHM6 | serine hydroxymethyltransferase 6 | chr1:7754599-7757087 FORWARD LENGTH=599)

HSP 1 Score: 74.3294 bits (181), Expect = 4.091e-18
Identity = 37/74 (50.00%), Postives = 46/74 (62.16%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXX-----ENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P  G+IFY+                 N  YDFE+KINF+VFPSL GGPHN+ I ALA+ALKQA +P +K Y +Q
Sbjct:  379 PRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAALAIALKQAASPEYKLYMRQ 452

HSP 2 Score: 33.8834 bits (76), Expect = 4.091e-18
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPR 50
            CDIVT+TTHKSLR PR
Sbjct:  365 CDIVTSTTHKSLRGPR 380

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT4G37930.1 (| Symbols: SHM1, STM, SHMT1 | serine transhydroxymethyltransferase 1 | chr4:17831891-17834742 REVERSE LENGTH=517)

HSP 1 Score: 75.8702 bits (185), Expect = 6.933e-18
Identity = 40/69 (57.97%), Postives = 44/69 (63.77%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P   MIF+R           E  +YDFEDKIN AVFP L GGPHNH I  LAVALKQATT  +KAY +Q
Sbjct:  291 PRGAMIFFRKGVKEINKQGKE-VLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQ 358

HSP 2 Score: 31.5722 bits (70), Expect = 6.933e-18
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  278 DVVTTTTHKSLRGPR 292

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT1G36370.1 (| Symbols: SHM7 | serine hydroxymethyltransferase 7 | chr1:13696240-13698576 REVERSE LENGTH=598)

HSP 1 Score: 73.9442 bits (180), Expect = 1.517e-17
Identity = 38/74 (51.35%), Postives = 45/74 (60.81%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAV-----YDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P  G+IFYR            +       YD E+KINFAVFPSL GGPHN+ I ALA+ALKQ  TP +KAY +Q
Sbjct:  375 PRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQVATPEYKAYIQQ 448

HSP 2 Score: 32.3426 bits (72), Expect = 1.517e-17
Identity = 13/16 (81.25%), Postives = 14/16 (87.50%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPR 50
            CDIVT+TTHK LR PR
Sbjct:  361 CDIVTSTTHKGLRGPR 376

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT5G26780.2 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 74.7146 bits (182), Expect = 1.521e-17
Identity = 41/76 (53.95%), Postives = 47/76 (61.84%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ--R*CAK 267
            P   MIF+R           E  +YD+ED+IN AVFP L GGPHNH I  LAVALKQA TP +KAY  Q  R C+K
Sbjct:  291 PRGAMIFFRKGLKEINKQGKE-VMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSK 365

HSP 2 Score: 31.5722 bits (70), Expect = 1.521e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  278 DVVTTTTHKSLRGPR 292

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT5G26780.3 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 74.7146 bits (182), Expect = 1.521e-17
Identity = 41/76 (53.95%), Postives = 47/76 (61.84%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ--R*CAK 267
            P   MIF+R           E  +YD+ED+IN AVFP L GGPHNH I  LAVALKQA TP +KAY  Q  R C+K
Sbjct:  291 PRGAMIFFRKGLKEINKQGKE-VMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSK 365

HSP 2 Score: 31.5722 bits (70), Expect = 1.521e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  278 DVVTTTTHKSLRGPR 292

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT5G26780.1 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=517)

HSP 1 Score: 74.7146 bits (182), Expect = 1.522e-17
Identity = 41/76 (53.95%), Postives = 47/76 (61.84%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ--R*CAK 267
            P   MIF+R           E  +YD+ED+IN AVFP L GGPHNH I  LAVALKQA TP +KAY  Q  R C+K
Sbjct:  291 PRGAMIFFRKGLKEINKQGKE-VMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSK 365

HSP 2 Score: 31.5722 bits (70), Expect = 1.522e-17
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:    6 DIVTTTTHKSLRDPR 50
            D+VTTTTHKSLR PR
Sbjct:  278 DVVTTTTHKSLRGPR 292

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT4G13890.1 (| Symbols: SHM5, EDA36, EDA37 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr4:8032146-8033718 REVERSE LENGTH=470)

HSP 1 Score: 85.1149 bits (209), Expect = 2.633e-17
Identity = 46/83 (55.42%), Postives = 51/83 (61.45%), Query Frame = 1

Query:    4 VTLSQQQPTRA*GTPGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            VT S  +  R    P AGMIFYR           E  VYDF+ KIN AVFP+L  GPHN++IGALAVALKQ   P FK YAKQ
Sbjct:  238 VTTSTHKSLRG---PRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALAVALKQVMAPSFKVYAKQ 317

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT4G32520.2 (| Symbols: SHM3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 65.0846 bits (157), Expect = 7.736e-16
Identity = 36/69 (52.17%), Postives = 40/69 (57.97%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P  GMIF+R            N V D E  +N AVFP L GGPHNH IG LAV LK A +P FKAY K+
Sbjct:  319 PRGGMIFFRKDPI--------NGV-DLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQKR 378

HSP 2 Score: 35.4242 bits (80), Expect = 7.736e-16
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPR 50
            CDIVTTTTHKSLR PR
Sbjct:  305 CDIVTTTTHKSLRGPR 320

[Close]

BLAST of EH059126 vs. TAIR peptide
Match: AT4G32520.1 (| Symbols: SHM3, AtSHMT3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 65.0846 bits (157), Expect = 7.736e-16
Identity = 36/69 (52.17%), Postives = 40/69 (57.97%), Query Frame = 1

Query:   46 PGAGMIFYRXXXXXXXXXXXENAVYDFEDKINFAVFPSLHGGPHNHQIGALAVALKQATTPGFKAYAKQ 252
            P  GMIF+R            N V D E  +N AVFP L GGPHNH IG LAV LK A +P FKAY K+
Sbjct:  319 PRGGMIFFRKDPI--------NGV-DLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQKR 378

HSP 2 Score: 35.4242 bits (80), Expect = 7.736e-16
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3

Query:    3 CDIVTTTTHKSLRDPR 50
            CDIVTTTTHKSLR PR
Sbjct:  305 CDIVTTTTHKSLRGPR 320

The following BLAST results are available for this feature:

BLAST of EH059126 vs. SwissProt
BLAST of EH059126 vs. TrEMBL
BLAST of EH059126 vs. TAIR peptide

BLAST of EH059126 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GLYM_SOLTU	2.262e-17	57.97	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_PEA	6.457e-17	56.52	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_ARATH	6.457e-17	57.97	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYC_NEUCR	1.093e-16	52.17	Serine hydroxymethyltransferase, cytosolic OS=Neur...	[more]
GLYN_FLAPR	1.418e-16	56.52	Serine hydroxymethyltransferase 2, mitochondrial O...	[more]
GLYM_FLAPR	2.395e-16	56.52	Serine hydroxymethyltransferase 1, mitochondrial O...	[more]
GLYC_CANAL	3.119e-16	50.72	Serine hydroxymethyltransferase, cytosolic OS=Cand...	[more]
GLYC_ASHGO	4.054e-16	53.03	Serine hydroxymethyltransferase, cytosolic OS=Ashb...	[more]
GLYC_YEAST	5.269e-16	50.72	Serine hydroxymethyltransferase, cytosolic OS=Sacc...	[more]
GLYM_KLULA	1.949e-15	54.55	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]

BLAST of EH059126 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FPJ3_ARATH	4.593e-26	75.36	Serine hydroxymethyltransferase OS=Arabidopsis tha...	[more]
Q8LBY1_ARATH	4.593e-26	75.36	Serine hydroxymethyltransferase OS=Arabidopsis tha...	[more]
O23254_ARATH	4.593e-26	75.36	Serine hydroxymethyltransferase OS=Arabidopsis tha...	[more]
D7MHA0_ARALY	4.593e-26	75.36	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q0ISV6_ORYSJ	8.336e-25	72.46	Os11g0455800 protein (Fragment) OS=Oryza sativa su...	[more]
C5Y297_SORBI	8.344e-25	72.46	Serine hydroxymethyltransferase OS=Sorghum bicolor...	[more]
A3CB05_ORYSJ	8.352e-25	72.46	Serine hydroxymethyltransferase OS=Oryza sativa su...	[more]
A2ZDY5_ORYSI	8.392e-25	72.46	Serine hydroxymethyltransferase OS=Oryza sativa su...	[more]
Q58A18_CUCME	8.406e-25	72.46	Putative uncharacterized protein 6F11 (Fragment) O...	[more]
Q2R4Y7_ORYSJ	8.450e-25	72.46	Serine hydroxymethyltransferase, mitochondrial, pu...	[more]

BLAST of EH059126 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G13930.1	3.646e-28	75.36	\| Symbols: SHM4 \| serine hydroxymethyltransferase ...	[more]
AT1G22020.1	4.091e-18	50.00	\| Symbols: SHM6 \| serine hydroxymethyltransferase ...	[more]
AT4G37930.1	6.933e-18	57.97	\| Symbols: SHM1, STM, SHMT1 \| serine transhydroxym...	[more]
AT1G36370.1	1.517e-17	51.35	\| Symbols: SHM7 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.2	1.521e-17	53.95	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.3	1.521e-17	53.95	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.1	1.522e-17	53.95	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT4G13890.1	2.633e-17	55.42	\| Symbols: SHM5, EDA36, EDA37 \| Pyridoxal phosphat...	[more]
AT4G32520.2	7.736e-16	52.17	\| Symbols: SHM3 \| serine hydroxymethyltransferase ...	[more]
AT4G32520.1	7.736e-16	52.17	\| Symbols: SHM3, AtSHMT3 \| serine hydroxymethyltra...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 3..71 score: 1.6
IPR001085	Serine hydroxymethyltransferase	PFAM	PF00464	SHMT	coord: 3..71 score: 8.5
IPR015422	Pyridoxal phosphate-dependent transferase, major region, subdomain 2	GENE3D	3.90.1150.10	no description	coord: 36..71 score: 1.6
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 1..72 score: 8.3
None	No IPR available	SEG	seg	seg	coord: 12..22 score
None	No IPR available	SEG	seg	seg	coord: 72..83 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EH059126 ID=EH059126; Name=EH059126; organism=Cicer arietinum; type=EST; length=550bp
ACTGTGACATTGTCACAACAACAACCCACAAGAGCTTAAGGGACCCCAGG
AGCTGGTATGATCTTCTACCGGAAGGGACCTAAGCCACCCAAGAAGGGTC
AGCCCGAGAATGCAGTTTATGATTTTGAAGACAAAATCAACTTTGCTGTT
TTCCCCTCCCTTCATGGTGGTCCTCACAACCACCAGATTGGTGCCCTTGC
TGTAGCTTTGAAACAGGCCACGACGCCCGGATTCAAGGCATATGCCAAGC
AACGTTAATGTGCCAAATGCAGTTTGCACTTGGAACATTACATTGATCAT
TAAATGGGATACAGTCCATTGTCACTTGGCAGGCAACTGAAAAATCATCA
TTGATCTTGTTGGGAATCTTAGACACTTCTTGGCTTTGACCTGGAAAATA
AATGATGGAAAAACCTTTTGTGAATCTACTGATAACCATCACCTGTTAAA
CACTGAATTGCTTAGTTTATCGAGTGATCTCACGTGCCCTTTACCTCTTG
AAGTGCGTAAATTACTATGCTACCCCCACGTCTCATCACCTTCCAAAGTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004372	glycine hydroxymethyltransferase activity
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006544	glycine metabolic process
GO:0006563	L-serine metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001085	Ser_HO-MeTrfase
IPR015422	PyrdxlP-dep_Trfase_small
IPR015424	PyrdxlP-dep_Trfase

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