FE669000

EST Overview
NameFE669000
Unique NameFE669000
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length448
Libraries
Library NameType
LIBEST_022821 Chickpea leave librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of FE669000 vs. SwissProt
Match: ALLB3_BETVE (Calcium-binding allergen Bet v 3 OS=Betula verrucosa GN=BETVIII PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 9.891e-18
Identity = 38/66 (57.58%), Postives = 54/66 (81.82%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            IFD+FDKN D +ITV+E+S+AL +LGLE +  ELES +KS+ ++GN GL +EDF+ LH+SL ++YF
Sbjct:   44 IFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISLHQSLNDSYF 109
[Close]
BLAST of FE669000 vs. SwissProt
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.292e-17
Identity = 39/67 (58.21%), Postives = 54/67 (80.60%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD  ITVEE+SQALT LGL A+  +L+S ++SYI+ GN GL ++DF  LH++L +++FG
Sbjct:   33 IFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFG 99
[Close]
BLAST of FE669000 vs. SwissProt
Match: CAST_SOLTU (Calcium-binding protein CAST OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.156e-16
Identity = 38/67 (56.72%), Postives = 52/67 (77.61%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FD+N D +I+VEE+SQAL +LGL+A+  E+ESM+K +IK  N GL +EDF  LH SL + +FG
Sbjct:   44 IFDVFDRNHDCLISVEELSQALNLLGLDADLSEIESMVKLHIKPENTGLRFEDFETLHRSLNDVFFG 110
[Close]
BLAST of FE669000 vs. SwissProt
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.579e-15
Identity = 35/67 (52.24%), Postives = 51/67 (76.12%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            +FD+FDKN D  ITVEE+SQAL+ LGL+A+F +L+S + S+IK    GL ++DF  LH++L  ++FG
Sbjct:   32 VFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAALHKTLDESFFG 98
[Close]
BLAST of FE669000 vs. SwissProt
Match: CML24_ORYSJ (Probable calcium-binding protein CML24 OS=Oryza sativa subsp. japonica GN=CML24 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.293e-13
Identity = 33/67 (49.25%), Postives = 48/67 (71.64%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            +FD+FD+NGD  ITV+E++QAL  LGLEA+   L + + +++ DG  GL +EDF  LH +LG+  FG
Sbjct:   43 VFDLFDRNGDGEITVDELAQALDALGLEADRAGLAATVGAHVPDGAAGLRFEDFESLHRALGDALFG 109
[Close]
BLAST of FE669000 vs. SwissProt
Match: CML32_ORYSJ (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica GN=CML32 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.251e-12
Identity = 32/67 (47.76%), Postives = 47/67 (70.15%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            +FD+FD+NGD  ITV+E++QAL  LGL A+   L + + +Y+ +G  GL +EDF  LH +LG+  FG
Sbjct:   38 VFDLFDRNGDGEITVDELAQALDALGLVADRDGLAATVSAYVPEGAAGLRFEDFDALHRALGDALFG 104
[Close]
BLAST of FE669000 vs. SwissProt
Match: CML27_ORYSJ (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica GN=CML27 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 6.866e-11
Identity = 31/67 (46.27%), Postives = 44/67 (65.67%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            +FD+FD+NGD  IT++E++ AL  LGL A+   LE+ +  YI  G  GL + DF  LH +LG+  FG
Sbjct:   35 VFDLFDRNGDGEITLDEMASALDALGLGADRAGLEATVGGYIPAGAAGLRFGDFEALHRALGDALFG 101
[Close]
BLAST of FE669000 vs. TrEMBL
Match: B9MTS7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_676209 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.274e-19
Identity = 45/67 (67.16%), Postives = 59/67 (88.06%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD MIT+EEISQAL++LGLEA+F +LE  IKS+IK G+ GL++EDFV LH+SL +++FG
Sbjct:   31 IFDLFDKNGDGMITIEEISQALSLLGLEADFSDLEFTIKSHIKPGSSGLSFEDFVSLHQSLDSSFFG 97
[Close]
BLAST of FE669000 vs. TrEMBL
Match: B9IGY1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_907177 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.664e-19
Identity = 45/67 (67.16%), Postives = 58/67 (86.57%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD MIT++EISQAL++LGL+A+F ELE  IKS+IK  N GL++EDFV LH+SL N++FG
Sbjct:   19 IFDLFDKNGDGMITIQEISQALSLLGLDADFSELEFTIKSHIKPDNNGLSFEDFVSLHQSLHNSFFG 85
[Close]
BLAST of FE669000 vs. TrEMBL
Match: Q6ZXI3_POPCA (Putative calmodulin-like protein OS=Populus canadensis PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.838e-19
Identity = 44/67 (65.67%), Postives = 59/67 (88.06%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD MIT+EEISQAL++LGL+A+F +LE  IKS+IK G+ GL++EDFV LH+SL +++FG
Sbjct:   31 IFDLFDKNGDGMITIEEISQALSLLGLDADFSDLEFTIKSHIKPGSSGLSFEDFVSLHQSLDSSFFG 97
[Close]
BLAST of FE669000 vs. TrEMBL
Match: B9T0Q0_RICCO (Calcium binding protein/cast, putative OS=Ricinus communis GN=RCOM_0126810 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.402e-18
Identity = 42/66 (63.64%), Postives = 56/66 (84.85%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            IFD+FDKNGD MITV+++SQAL++LGLEA+F ELES I+S+I+ GN+GL +EDF  LH+SL   +F
Sbjct:   41 IFDLFDKNGDGMITVQDLSQALSLLGLEADFSELESTIRSHIRPGNDGLAFEDFFSLHQSLDEAFF 106
[Close]
BLAST of FE669000 vs. TrEMBL
Match: A0FIJ7_CITSI (Ca2+-binding protein OS=Citrus sinensis PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.138e-18
Identity = 43/66 (65.15%), Postives = 53/66 (80.30%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            +FDMFDKNGD MITV+E+ QAL +LGLE +  ELES I S++K GN+GL +EDFV LHESL  T+F
Sbjct:   44 VFDMFDKNGDGMITVKELHQALNLLGLETDLSELESTIASHVKPGNDGLEFEDFVSLHESLDETFF 109
[Close]
BLAST of FE669000 vs. TrEMBL
Match: Q9FR00_TOBAC (Avr9/Cf-9 rapidly elicited protein 31 OS=Nicotiana tabacum GN=ACRE31 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.917e-17
Identity = 40/67 (59.70%), Postives = 55/67 (82.09%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FD+N DS+I+V+E+SQAL +LGL+A+  E+ESM++SYIK GN GL +EDF  LH SL + +FG
Sbjct:   48 IFDVFDRNHDSLISVDELSQALNLLGLDADQSEIESMVRSYIKSGNNGLRFEDFEALHRSLDDVFFG 114
[Close]
BLAST of FE669000 vs. TrEMBL
Match: D7MFI4_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_658045 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.543e-16
Identity = 38/67 (56.72%), Postives = 54/67 (80.60%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD  ITVEE+SQAL+ LGL A+  +L+S ++SYI+ GN GL ++DF  LH++L +++FG
Sbjct:   32 IFDLFDKNGDGFITVEELSQALSRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFG 98
[Close]
BLAST of FE669000 vs. TrEMBL
Match: D7TKX6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_56.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029852001 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.241e-15
Identity = 38/66 (57.58%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            IFD+FDKN D  ITV E+SQAL +LGL+A+  +L+S+++S+IK GN GL ++DF  LH SL +TYF
Sbjct:  195 IFDLFDKNSDGTITVTELSQALALLGLDADLPDLQSIVQSHIKPGNLGLEFDDFAALHLSLNDTYF 260
[Close]
BLAST of FE669000 vs. TrEMBL
Match: C5DB59_VITVI (Calcium binding OS=Vitis vinifera GN=40N03_10 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.241e-15
Identity = 38/66 (57.58%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            IFD+FDKN D  ITV E+SQAL +LGL+A+  +L+S+++S+IK GN GL ++DF  LH SL +TYF
Sbjct:   33 IFDLFDKNSDGTITVTELSQALALLGLDADLPDLQSIVQSHIKPGNLGLEFDDFAALHLSLNDTYF 98
[Close]
BLAST of FE669000 vs. TrEMBL
Match: A9CSL0_9ROSI (Putative uncharacterized protein (Fragment) OS=Vitis hybrid cultivar PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.241e-15
Identity = 38/66 (57.58%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYF 440
            IFD+FDKN D  ITV E+SQAL +LGL+A+  +L+S+++S+IK GN GL ++DF  LH SL +TYF
Sbjct:   26 IFDLFDKNSDGTITVTELSQALALLGLDADLPDLQSIVQSHIKPGNLGLEFDDFAALHLSLNDTYF 91
[Close]
BLAST of FE669000 vs. TAIR peptide
Match: AT4G20780.1 (| Symbols: CML42 | calmodulin like 42 | chr4:11133309-11133884 REVERSE LENGTH=191)

HSP 1 Score: 88.5817 bits (218), Expect = 1.540e-18
Identity = 39/67 (58.21%), Postives = 54/67 (80.60%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            IFD+FDKNGD  ITVEE+SQALT LGL A+  +L+S ++SYI+ GN GL ++DF  LH++L +++FG
Sbjct:   33 IFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFG 99
[Close]
BLAST of FE669000 vs. TAIR peptide
Match: AT5G44460.1 (| Symbols: CML43 | calmodulin like 43 | chr5:17917286-17917831 FORWARD LENGTH=181)

HSP 1 Score: 81.6481 bits (200), Expect = 1.883e-16
Identity = 35/67 (52.24%), Postives = 51/67 (76.12%), Query Frame = 3 
Query:  243 IFDMFDKNGDSMITVEEISQALTMLGLEAEFKELESMIKSYIKDGNEGLTYEDFVGLHESLGNTYFG 443
            +FD+FDKN D  ITVEE+SQAL+ LGL+A+F +L+S + S+IK    GL ++DF  LH++L  ++FG
Sbjct:   32 VFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAALHKTLDESFFG 98
The following BLAST results are available for this feature:
BLAST of FE669000 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7
Match NameE-valueIdentityDescription
ALLB3_BETVE9.891e-1857.58Calcium-binding allergen Bet v 3 OS=Betula verruco... [more]
CML42_ARATH1.292e-1758.21Calcium-binding protein CML42 OS=Arabidopsis thali... [more]
CAST_SOLTU4.156e-1656.72Calcium-binding protein CAST OS=Solanum tuberosum ... [more]
CML43_ARATH1.579e-1552.24Probable calcium-binding protein CML43 OS=Arabidop... [more]
CML24_ORYSJ3.293e-1349.25Probable calcium-binding protein CML24 OS=Oryza sa... [more]
CML32_ORYSJ1.251e-1247.76Probable calcium-binding protein CML32 OS=Oryza sa... [more]
CML27_ORYSJ6.866e-1146.27Probable calcium-binding protein CML27 OS=Oryza sa... [more]
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BLAST of FE669000 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9MTS7_POPTR1.274e-1967.16Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9IGY1_POPTR1.664e-1967.16Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q6ZXI3_POPCA2.838e-1965.67Putative calmodulin-like protein OS=Populus canade... [more]
B9T0Q0_RICCO2.402e-1863.64Calcium binding protein/cast, putative OS=Ricinus ... [more]
A0FIJ7_CITSI3.138e-1865.15Ca2+-binding protein OS=Citrus sinensis PE=2 SV=1[more]
Q9FR00_TOBAC5.917e-1759.70Avr9/Cf-9 rapidly elicited protein 31 OS=Nicotiana... [more]
D7MFI4_ARALY6.543e-1656.72Predicted protein OS=Arabidopsis lyrata subsp. lyr... [more]
D7TKX6_VITVI4.241e-1557.58Whole genome shotgun sequence of line PN40024, sca... [more]
C5DB59_VITVI4.241e-1557.58Calcium binding OS=Vitis vinifera GN=40N03_10 PE=4... [more]
A9CSL0_9ROSI4.241e-1557.58Putative uncharacterized protein (Fragment) OS=Vit... [more]
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BLAST of FE669000 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G20780.11.540e-1858.21| Symbols: CML42 | calmodulin like 42 | chr4:11133... [more]
AT5G44460.11.883e-1652.24| Symbols: CML43 | calmodulin like 43 | chr5:17917... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048Calcium-binding EF-handSMARTSM00054no descriptioncoord: 46..74
score: 0.00051coord: 82..110
score
IPR011992EF-hand-like domainGENE3D1.10.238.10no descriptioncoord: 47..106
score: 9
IPR018248EF-handPFAMPF00036efhandcoord: 47..74
score: 5.3
IPR018249EF-HAND 2PROFILEPS50222EF_HAND_2coord: 42..77
score: 14.179coord: 78..113
score: 6
NoneNo IPR availablePANTHERPTHR10891CALMODULINcoord: 46..116
score: 5.6
NoneNo IPR availablePANTHERPTHR10891:SF43CALCIUM BINDING PROTEIN/CASTcoord: 46..116
score: 5.6
NoneNo IPR availableSEGsegsegcoord: 32..49
score
NoneNo IPR availableSUPERFAMILY47473EF-handcoord: 47..107
score: 1.8
NoneNo IPR availableSEGsegsegcoord: 70..83
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FE669000 ID=FE669000; Name=FE669000; organism=Cicer arietinum; type=EST; length=448bp
CATATTTATAGGTTTATTAGGATTTTTCCAGTTTTGTTTTCCAATATACA
CAAATCTATCTATCAATCCAACAACACAATACCCACTTCTTCTAACTCCT
AGAGTTTCTTCAACTCCTTCATCGCTGCACGCACGCGCTTCTAATTCACG
CGCTAGGGGTAATGGGGCAACAGAAAATAAACATGACATCATCGTCGTTT
AGGCTTCGATGTCGGAGTCTCAACTCGCTCCGTCTCCGAAGAATATTTGA
CATGTTCGACAAAAACGGTGATTCTATGATAACAGTAGAGGAAATAAGCC
AGGCGTTGACCATGTTGGGATTGGAAGCAGAGTTCAAAGAGCTTGAATCC
ATGATCAAGTCGTACATAAAGGATGGCAACGAAGGGCTAACGTATGAGGA
TTTCGTAGGTTTGCACGAATCTCTTGGGAACACATATTTTGGAGTGGA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018248EF-hand
IPR018249EF-HAND 2