FE669372

EST Overview


Name	FE669372
Unique Name	FE669372
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	459

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP3_PRUDU (Non-specific lipid-transfer protein 3 OS=Prunus dulcis PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.749e-10
Identity = 27/49 (55.10%), Postives = 37/49 (75.51%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            ++C+C+K AV+  G  Y+N N  LAA  P +CGVNIPY+ISP+TDC S+
Sbjct:   76 SICNCLKQAVN--GIPYTNANAGLAAGLPGKCGVNIPYKISPSTDCKSI 122

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP_BETVU (Non-specific lipid-transfer protein OS=Beta vulgaris GN=IWF1' PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.445e-8
Identity = 25/49 (51.02%), Postives = 35/49 (71.43%), Query Frame = -2

Query:  309 CSCIKNAVSS-SGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSVH 452
            C+C+K+A +S  G  Y       AAS PR+CGV++PY ISPNT+CN++H
Sbjct:   74 CTCLKSAATSIKGINYGK-----AASLPRQCGVSVPYAISPNTNCNAIH 117

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP2_GOSHI (Non-specific lipid-transfer protein OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 7.582e-8
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C CIK+A  ++G T    N  LA+  P +CGVNIPY+ISP+TDCNSV
Sbjct:   75 AACRCIKSA--AAGIT--GINFGLASGLPGKCGVNIPYKISPSTDCNSV 119

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP1_GOSHI (Non-specific lipid-transfer protein OS=Gossypium hirsutum PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 7.582e-8
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C CIK+A  ++G T    N  LA+  P +CGVNIPY+ISP+TDCNSV
Sbjct:   71 AACRCIKSA--AAGIT--GINFGLASGLPGKCGVNIPYKISPSTDCNSV 115

[Close]

BLAST of FE669372 vs. SwissProt
Match: LTP1_MORNI (Non-specific lipid-transfer protein 1 OS=Morus nigra PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.689e-7
Identity = 25/49 (51.02%), Postives = 34/49 (69.39%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C+C+K+A +S        NL LAA  P +CGV++PY+ISP+TDC SV
Sbjct:   46 AACNCLKSAFNS----IKGLNLNLAAGLPGKCGVSVPYKISPSTDCKSV 90

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP_HELAN (Non-specific lipid-transfer protein OS=Helianthus annuus PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.206e-7
Identity = 26/49 (53.06%), Postives = 34/49 (69.39%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C C+K+A +S     S  N   AASFP +CGV+IPY+ISP+TDC+ V
Sbjct:   71 AACGCLKSAYNS----ISGVNAGNAASFPGKCGVSIPYKISPSTDCSKV 115

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP2_SOLCI (Non-specific lipid-transfer protein 2 OS=Solanum chilense PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.763e-7
Identity = 25/49 (51.02%), Postives = 35/49 (71.43%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C+C+K+A SS    +++ +L  AAS P  C VNIPY+ISP+TDC+ V
Sbjct:   69 AACACLKSAASS----FTDLDLGKAASLPSTCNVNIPYKISPSTDCSKV 113

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP_DAUCA (Non-specific lipid-transfer protein OS=Daucus carota GN=EP2 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 8.383e-7
Identity = 24/47 (51.06%), Postives = 29/47 (61.70%), Query Frame = -2

Query:  312 CSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 452
            C C+K   ++     +  NL  AA  P RCGVNIPY+ISP TDCN V
Sbjct:   77 CGCLKQTANA----VTGLNLNAAAGLPARCGVNIPYKISPTTDCNRV 119

[Close]

BLAST of FE669372 vs. SwissProt
Match: NLTP5_LENCU (Non-specific lipid-transfer protein 5 OS=Lens culinaris PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 8.383e-7
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A C+C+K+A SS   T  NTN   AA+ P +CGVNIPY+IS +T+CN+V
Sbjct:   71 AACNCMKSAASS--ITKLNTNN--AAALPGKCGVNIPYKISTSTNCNTV 115

[Close]

BLAST of FE669372 vs. TrEMBL
Match: C6SVW1_SOYBN (Non-specific lipid-transfer protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 7.847e-14
Identity = 35/49 (71.43%), Postives = 41/49 (83.67%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            AVC+CIKN V +SGF YS+ NL LAA+ P++CGVNIPYQISPNTDC  V
Sbjct:   73 AVCNCIKNGVRNSGFNYSDFNLNLAANLPKKCGVNIPYQISPNTDCTRV 121

[Close]

BLAST of FE669372 vs. TrEMBL
Match: C6TNW2_SOYBN (Non-specific lipid-transfer protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.338e-13
Identity = 35/49 (71.43%), Postives = 41/49 (83.67%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            AVC+CIKNAV +SGFTYS+ N  LAA+ P+ CGVNIPYQISP+TDC  V
Sbjct:   81 AVCNCIKNAVRNSGFTYSHFNFNLAANLPKNCGVNIPYQISPDTDCTRV 129

[Close]

BLAST of FE669372 vs. TrEMBL
Match: B6CQU4_9ROSA (Non-specific lipid-transfer protein OS=Prunus dulcis x Prunus persica GN=Pru du 3.02 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 7.600e-9
Identity = 27/49 (55.10%), Postives = 37/49 (75.51%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            ++C+C+K AV+  G  Y+N N  LAA  P +CGVNIPY+ISP+TDC S+
Sbjct:   76 SICNCLKQAVN--GIPYTNANAGLAAGLPGKCGVNIPYKISPSTDCKSI 122

[Close]

BLAST of FE669372 vs. TrEMBL
Match: Q8H2B3_PRUPE (Non-specific lipid-transfer protein OS=Prunus persica GN=LTP2 PE=2 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 1.296e-8
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            ++C+C+K AV+  G  Y N N  LAA  P +CGVNIPY+ISP+TDC S+
Sbjct:   76 SICNCLKQAVN--GIPYPNANAGLAAGLPGKCGVNIPYKISPSTDCKSI 122

[Close]

BLAST of FE669372 vs. TrEMBL
Match: C0L0I5_PRUDU (Non-specific lipid-transfer protein OS=Prunus dulcis PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.693e-8
Identity = 26/49 (53.06%), Postives = 37/49 (75.51%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            ++C+C+K AV+  G  Y+N N  LAA  P +CGVNIPY+ISP+TDC ++
Sbjct:   76 SICNCLKQAVN--GIPYTNANAGLAAGLPGKCGVNIPYKISPSTDCKTI 122

[Close]

BLAST of FE669372 vs. TrEMBL
Match: Q52RN7_LEOAR (Non-specific lipid-transfer protein OS=Leonurus artemisia GN=Afp PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.888e-8
Identity = 29/50 (58.00%), Postives = 34/50 (68.00%), Query Frame = -2

Query:  309 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSVH 458
            AVC+C+K    S    YS  NL  AA  P +CGV+IPYQISPNTDC+ VH
Sbjct:   70 AVCNCLKTLAKS----YSGINLGNAAGLPGKCGVSIPYQISPNTDCSKVH 115

[Close]

BLAST of FE669372 vs. TrEMBL
Match: C6SX11_SOYBN (Non-specific lipid-transfer protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.888e-8
Identity = 29/48 (60.42%), Postives = 36/48 (75.00%), Query Frame = -2

Query:  312 VCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 455
            VC CIKNAVS+  F Y  +N+  AA+ P++CGVNIP QISP+TDC  V
Sbjct:   79 VCKCIKNAVSA--FHYGKSNVDRAAALPKQCGVNIPCQISPSTDCTRV 124

[Close]

BLAST of FE669372 vs. TrEMBL
Match: B9IGS4_POPTR (Non-specific lipid-transfer protein OS=Populus trichocarpa GN=POPTRDRAFT_668194 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.097e-7
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            +VC+C+KN  S   F+Y++ N+ LAA  P +CGV +PY+I P+TDC SV
Sbjct:   73 SVCTCLKNTASQ--FSYNSRNVALAAGLPGKCGVKLPYKIDPSTDCKSV 119

[Close]

BLAST of FE669372 vs. TrEMBL
Match: A9PH75_POPTR (Non-specific lipid-transfer protein OS=Populus trichocarpa GN=POPTRDRAFT_668195 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.097e-7
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            +VC+C+KN  S   F+Y++ N+ LAA  P +CGV +PY+I P+TDC SV
Sbjct:   73 SVCTCLKNTASQ--FSYNSRNVALAAGLPGKCGVKLPYKIDPSTDCKSV 119

[Close]

BLAST of FE669372 vs. TrEMBL
Match: A5JV01_SESIN (Non-specific lipid-transfer protein OS=Sesamum indicum GN=LTP5 PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.872e-7
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = -2

Query:  312 AVCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 458
            A CSC+K   S    TY   NL  AA  P++CGVNIPY+ISP+TDC+ V
Sbjct:   73 ATCSCLKTLAS----TYKGVNLSKAAGLPQQCGVNIPYKISPSTDCSKV 117

[Close]

BLAST of FE669372 vs. TAIR peptide
Match: AT5G01870.1 (| Symbols: | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | chr5:337234-337678 FORWARD LENGTH=116)

HSP 1 Score: 51.6026 bits (122), Expect = 2.233e-7
Identity = 23/48 (47.92%), Postives = 33/48 (68.75%), Query Frame = -2

Query:  312 VCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 455
            VC CIK+ V     +YS+  L  AA+ P +CGV +PY+I P+T+CNS+
Sbjct:   70 VCRCIKSVVGR--VSYSSIYLKKAAALPGKCGVKLPYKIDPSTNCNSI 115

[Close]

BLAST of FE669372 vs. TAIR peptide
Match: AT3G08770.1 (| Symbols: LTP6 | lipid transfer protein 6 | chr3:2664349-2664784 REVERSE LENGTH=113)

HSP 1 Score: 51.6026 bits (122), Expect = 2.233e-7
Identity = 22/48 (45.83%), Postives = 32/48 (66.67%), Query Frame = -2

Query:  312 VCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNSV 455
            VC CIK+A+   G T S   +  A   P +CGV++PY+ SP+TDC+S+
Sbjct:   67 VCRCIKSAIG--GLTLSPRTIQNALELPSKCGVDLPYKFSPSTDCDSI 112

[Close]

BLAST of FE669372 vs. TAIR peptide
Match: AT3G08770.2 (| Symbols: LTP6 | lipid transfer protein 6 | chr3:2664330-2664784 REVERSE LENGTH=117)

HSP 1 Score: 50.447 bits (119), Expect = 4.974e-7
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = -2

Query:  315 VCSCIKNAVSSSGFTYSNTNLYLAASFPRRCGVNIPYQISPNTDCNS 455
            VC CIK+A+   G T S   +  A   P +CGV++PY+ SP+TDC+S
Sbjct:   67 VCRCIKSAIG--GLTLSPRTIQNALELPSKCGVDLPYKFSPSTDCDS 111

The following BLAST results are available for this feature:

BLAST of FE669372 vs. SwissProt
BLAST of FE669372 vs. TrEMBL
BLAST of FE669372 vs. TAIR peptide

BLAST of FE669372 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
NLTP3_PRUDU	4.749e-10	55.10	Non-specific lipid-transfer protein 3 OS=Prunus du...	[more]
NLTP_BETVU	4.445e-8	51.02	Non-specific lipid-transfer protein OS=Beta vulgar...	[more]
NLTP2_GOSHI	7.582e-8	57.14	Non-specific lipid-transfer protein OS=Gossypium h...	[more]
NLTP1_GOSHI	7.582e-8	57.14	Non-specific lipid-transfer protein OS=Gossypium h...	[more]
LTP1_MORNI	1.689e-7	51.02	Non-specific lipid-transfer protein 1 OS=Morus nig...	[more]
NLTP_HELAN	2.206e-7	53.06	Non-specific lipid-transfer protein OS=Helianthus ...	[more]
NLTP2_SOLCI	3.763e-7	51.02	Non-specific lipid-transfer protein 2 OS=Solanum c...	[more]
NLTP_DAUCA	8.383e-7	51.06	Non-specific lipid-transfer protein OS=Daucus caro...	[more]
NLTP5_LENCU	8.383e-7	55.10	Non-specific lipid-transfer protein 5 OS=Lens culi...	[more]

BLAST of FE669372 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SVW1_SOYBN	7.847e-14	71.43	Non-specific lipid-transfer protein OS=Glycine max...	[more]
C6TNW2_SOYBN	1.338e-13	71.43	Non-specific lipid-transfer protein OS=Glycine max...	[more]
B6CQU4_9ROSA	7.600e-9	55.10	Non-specific lipid-transfer protein OS=Prunus dulc...	[more]
Q8H2B3_PRUPE	1.296e-8	55.10	Non-specific lipid-transfer protein OS=Prunus pers...	[more]
C0L0I5_PRUDU	1.693e-8	53.06	Non-specific lipid-transfer protein OS=Prunus dulc...	[more]
Q52RN7_LEOAR	2.888e-8	58.00	Non-specific lipid-transfer protein OS=Leonurus ar...	[more]
C6SX11_SOYBN	2.888e-8	60.42	Non-specific lipid-transfer protein OS=Glycine max...	[more]
B9IGS4_POPTR	1.097e-7	51.02	Non-specific lipid-transfer protein OS=Populus tri...	[more]
A9PH75_POPTR	1.097e-7	51.02	Non-specific lipid-transfer protein OS=Populus tri...	[more]
A5JV01_SESIN	1.872e-7	55.10	Non-specific lipid-transfer protein OS=Sesamum ind...	[more]

BLAST of FE669372 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G01870.1	2.233e-7	47.92	\| Symbols: \| Bifunctional inhibitor/lipid-transfe...	[more]
AT3G08770.1	2.233e-7	45.83	\| Symbols: LTP6 \| lipid transfer protein 6 \| chr3:...	[more]
AT3G08770.2	4.974e-7	46.81	\| Symbols: LTP6 \| lipid transfer protein 6 \| chr3:...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000528	Plant lipid transfer protein/Par allergen	PRINTS	PR00382	LIPIDTRNSFER	coord: 19..36 score: 9.3e-07coord: 37..48 score: 9.3
IPR013770	Plant lipid transfer protein/hydrophobic protein, helical domain	GENE3D	1.10.110.10	no description	coord: 1..50 score: 5.2
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 1..50 score: 9.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE669372 ID=FE669372; Name=FE669372; organism=Cicer arietinum; type=EST; length=459bp
CCCAAATAAACAAAACACATCATTAGTTAATAAACAACAACAGATTACTC
ACATCCATCAACTTGTTCTACTTCTTAAGTCTCTATTACTGCCACACTTC
CCACAATAAGCTTACACAAATAGCACAAACCACACAGTACACACAAACAC
ACGATACAGTACAATAAAACTAGTACAAAGCCATAAAGAAACAACTTCAG
ATGATCATTCTCACGTCACTCATGAAAGTAGCCTCTTATTCCCACACACT
ATAATCTAACATAACTTCCCACTTCAGTGACTTCTCCACACGTCATGTTT
CAACATCAGTGCACACTGTTGCAGTCGGTATTGGGGCTGATCTGGTAAGG
AATGTTAACCCCACATCTCCTTGGAAAACTAGCAGCAAGGTAAAGGTTTG
TATTAGAGTAAGTGAAACCACTTGAGCTAACAGCATTCTTAATGCAGCTA
CAAACAGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006869	lipid transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0008289	lipid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000528	Plant_nsLTP
IPR013770	Plant lipid transfer protein/hydrophobic protein helical domain
IPR016140	Bifunc_inhib/LTP/seed_store

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