GR393338

EST Overview


Name	GR393338
Unique Name	GR393338
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	651

Libraries


Library Name	Type
LIBEST_024778 ICC1882 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR393338 vs. SwissProt
Match: ST7R_ARATH (7-dehydrocholesterol reductase OS=Arabidopsis thaliana GN=DWF5 PE=1 SV=1)

HSP 1 Score: 312.768 bits (800), Expect = 1.018e-84
Identity = 144/162 (88.89%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPV LG QLA++ILVAGILCIYINYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSGETK SLLLTSGWWGLARHFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYC++V YRIIPGIY
Sbjct:  271 VPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432

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BLAST of GR393338 vs. SwissProt
Match: DHCR7_XENTR (7-dehydrocholesterol reductase OS=Xenopus tropicalis GN=dhcr7 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 4.476e-40
Identity = 74/162 (45.68%), Postives = 106/162 (65.43%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV +PV L    A+ +L+ G++  YI    + Q+  FRRTNG   +WGK P  IE SYT+  G+   S L+ SG+WG+ARH +Y  +++ +  + +   F+H LPYFY  ++TILL  R  RD+ RC SKYGK WKLY D VPYR++PG++
Sbjct:  312 LPYLYTLQGLYLVYNPVELSTATAVGVLLLGLIGYYIFRMTNHQKDLFRRTNGNCKIWGKKPKSIECSYTSADGKRHYSKLMISGFWGVARHLNYTGDLMGSLAYCLACGFDHLLPYFYFTYMTILLVHRCIRDEHRCSSKYGKDWKLYTDAVPYRLLPGLF 473

[Close]

BLAST of GR393338 vs. SwissProt
Match: DHCR7_XENLA (7-dehydrocholesterol reductase OS=Xenopus laevis GN=dhcr7 PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 8.441e-39
Identity = 72/162 (44.44%), Postives = 104/162 (64.20%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV +PV L    A+ +L+ G++  YI    + Q+  FRRTNG   +WGK P  IE  Y +  G+   S L+ SG+WG+ARH +Y  +++ +  + +   F+H LPYFY I++TILL  R  RD+ RC SKYGK WKLY   VPYR++PG++
Sbjct:  312 LPYLYTLQGLYLVYNPVELSTTAAVAVLLLGLIGYYIFRMTNHQKDLFRRTNGNCKIWGKKPKSIECFYVSADGKRHYSKLMISGFWGVARHLNYTGDLMGSLAYCLACGFDHLLPYFYFIYMTILLVHRCIRDEHRCSSKYGKDWKLYTSAVPYRLLPGLF 473

[Close]

BLAST of GR393338 vs. SwissProt
Match: DHCR7_RAT (7-dehydrocholesterol reductase OS=Rattus norvegicus GN=Dhcr7 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.440e-38
Identity = 74/162 (45.68%), Postives = 105/162 (64.81%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV HPV L    AL +L+ G++  YI    + Q+  FRRT+G   +WGK P  IE SYT+  G   RS LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +KYG+ W+ Y   VPYR++PGI+
Sbjct:  310 LPYLYTLQGLYLVYHPVQLSTPNALGVLLLGLVGYYIFRMTNHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYVAAVPYRLLPGIF 471

[Close]

BLAST of GR393338 vs. SwissProt
Match: DHCR7_BOVIN (7-dehydrocholesterol reductase OS=Bos taurus GN=DHCR7 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.440e-38
Identity = 73/162 (45.06%), Postives = 105/162 (64.81%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV HPV L    AL +L+ G+L  YI    + Q+  FRRT+G+  +WG+ P  IE SYT+  G+   S LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+IF+ ILL  R  RD+ RC +KYG+ W+ Y   VPYR++PGI+
Sbjct:  314 LPYLYTLQGLYLVYHPVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRDWEHYTAAVPYRLLPGIF 475

[Close]

BLAST of GR393338 vs. SwissProt
Match: DHCR7_MOUSE (7-dehydrocholesterol reductase OS=Mus musculus GN=Dhcr7 PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 1.880e-38
Identity = 74/162 (45.68%), Postives = 105/162 (64.81%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV HPV L    AL IL+ G++  YI    + Q+  FRRT+G+  +WGK P  IE SYT+  G    S LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +KYG+ W+ Y   VPYR++PGI+
Sbjct:  310 LPYLYTLQGLYLVYHPVQLSTPNALGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYTAAVPYRLLPGIF 471

[Close]

BLAST of GR393338 vs. SwissProt
Match: DHCR7_HUMAN (7-dehydrocholesterol reductase OS=Homo sapiens GN=DHCR7 PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 1.880e-38
Identity = 72/162 (44.44%), Postives = 106/162 (65.43%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV HPV L    A+ +L+ G++  YI    + Q+  FRRT+G+  +WG+ P  IE SYT+  G+   S LL SG+WG+ARHF+YV +++ +  + +     H LPYFY+I++ ILL  R  RD+ RC SKYG+ W+ Y   VPYR++PGI+
Sbjct:  314 LPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF 475

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BLAST of GR393338 vs. SwissProt
Match: DHCR7_DANRE (7-dehydrocholesterol reductase OS=Danio rerio GN=dhcr7 PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 3.921e-36
Identity = 69/162 (42.59%), Postives = 103/162 (63.58%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P +YT  G+YLV +P+ L    A  +L+ G++  YI    + Q+  FRRT G  S+WGK P+ IE SY +  G   +S L+TSG+WG+ARH +Y  +++ +  + +    NH LPYFY+I++TILL  R  RD+ RC +KYGK W+ Y   V YR++P I+
Sbjct:  317 LPFLYTLQGLYLVYNPIQLSTPHAAGVLILGLVGYYIFRVTNHQKDLFRRTEGNCSIWGKKPTFIECSYQSADGAIHKSKLMTSGFWGVARHMNYTGDLMGSLAYCLACGGNHLLPYFYIIYMTILLVHRCIRDEHRCSNKYGKDWERYTAAVSYRLLPNIF 478

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BLAST of GR393338 vs. SwissProt
Match: LBR_RAT (Lamin-B receptor OS=Rattus norvegicus GN=Lbr PE=1 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.462e-30
Identity = 66/162 (40.74%), Postives = 92/162 (56.79%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VP  Y+    YLVNHP +L   L   +++A  LC Y+ + C          N + + + K P+  + ++  T   +    LL SGWWG  RH +Y+ +++ A  W++P  FNH LPYFYVI+ T LL  R  RD+ +CR KYG  W+ YC RVPYRI P IY
Sbjct:  469 VPFTYSLQAFYLVNHPQDLSWPLTS-VIIALKLCGYVIFRC---------ANSQKNAFRKNPTDPKLAHLKTIPTSTWKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYVIYFTALLIHREARDEHQCRRKYGLAWEKYCQRVPYRIFPYIY 620

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BLAST of GR393338 vs. SwissProt
Match: LBR_MOUSE (Lamin-B receptor OS=Mus musculus GN=Lbr PE=1 SV=2)

HSP 1 Score: 131.339 bits (329), Expect = 4.199e-30
Identity = 65/162 (40.12%), Postives = 93/162 (57.41%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VP  Y+    YLV+HP +L   LA  +++A  LC Y+ + C          N + + + K P+  + ++  T   +    LL SGWWG  RH +Y+ +++ A  W++P  FNH LPYFY+I+ T LL  R  RD+ +CR KYG  W+ YC RVPYRI P IY
Sbjct:  475 VPFTYSLQAFYLVSHPHDLSWPLAS-VIIALKLCGYVIFRC---------ANSQKNAFRKNPTDPKLAHLKTIHTSTGKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHLLPYFYIIYFTALLIHREARDEHQCRRKYGLAWEKYCQRVPYRIFPYIY 626

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BLAST of GR393338 vs. TrEMBL
Match: C6TIU6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 3.631e-90
Identity = 154/162 (95.06%), Postives = 160/162 (98.77%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPVNLGI+LAL ILVAGILCIYINYDCDRQRQEFRRTNGKG+VWGKAPSKIEA+YTTTSGETKRSLLLTSGWWGL+RHFHYVPEILAAFFWTVPALF HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCD+VPYRIIPGIY
Sbjct:  271 VPSVYTSPGMYLVNHPVNLGIKLALSILVAGILCIYINYDCDRQRQEFRRTNGKGTVWGKAPSKIEATYTTTSGETKRSLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFEHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDKVPYRIIPGIY 432

[Close]

BLAST of GR393338 vs. TrEMBL
Match: Q2QCX7_GOSHI (Sterol delta-7 reductase DWF5 OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 325.479 bits (833), Expect = 2.203e-87
Identity = 147/162 (90.74%), Postives = 157/162 (96.91%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPVNLG+QLAL+ILVAG+LCIYINYDCDRQRQEFRRTNGK  +WGKAPSKIEA+YTTTSGETK SLLLTSGWW LARHFHYVPEILAAFFWTVPALFNHFLPYFYV+FLTILLFDRAKRDDDRCRSKYGKYWKLYC +VPY+I+PGIY
Sbjct:  276 VPSVYTSPGMYLVNHPVNLGMQLALYILVAGVLCIYINYDCDRQRQEFRRTNGKCKIWGKAPSKIEATYTTTSGETKTSLLLTSGWWCLARHFHYVPEILAAFFWTVPALFNHFLPYFYVVFLTILLFDRAKRDDDRCRSKYGKYWKLYCTKVPYKIVPGIY 437

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BLAST of GR393338 vs. TrEMBL
Match: Q6S5A3_TROMA (Sterol delta-7 reductase OS=Tropaeolum majus PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 2.435e-86
Identity = 148/162 (91.36%), Postives = 156/162 (96.30%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPSIYTSPGMYLVNHPV+LG QLA++ILVAGILCIYINYDCDRQRQEFRRTNGK +VWGK+PSKI ASYTTTSGETK SLLLTSGWWGLARHFHYVPEIL+AFFWTVPALFNH LPYFYV+FLTILLFDRAKRDDDRCRSKYGKYWKLYC RVPY+IIPGIY
Sbjct:  274 VPSIYTSPGMYLVNHPVDLGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCTVWGKSPSKILASYTTTSGETKSSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFNHVLPYFYVVFLTILLFDRAKRDDDRCRSKYGKYWKLYCQRVPYKIIPGIY 435

[Close]

BLAST of GR393338 vs. TrEMBL
Match: B9HV17_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_833904 PE=4 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 7.834e-85
Identity = 145/162 (89.51%), Postives = 155/162 (95.68%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPVNLG+QLAL+IL AGIL IY+NYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSG+TK SLLLTSGWWGL+RHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYC++V YRI+PGIY
Sbjct:  273 VPSVYTSPGMYLVNHPVNLGLQLALYILAAGILSIYVNYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGQTKISLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEKVRYRIVPGIY 434

[Close]

BLAST of GR393338 vs. TrEMBL
Match: A9PC70_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 7.834e-85
Identity = 145/162 (89.51%), Postives = 155/162 (95.68%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPVNLG+QLAL+IL AGIL IY+NYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSG+TK SLLLTSGWWGL+RHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYC++V YRI+PGIY
Sbjct:  239 VPSVYTSPGMYLVNHPVNLGLQLALYILAAGILSIYVNYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGQTKISLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEKVRYRIVPGIY 400

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BLAST of GR393338 vs. TrEMBL
Match: C5Y133_SORBI (Putative uncharacterized protein Sb04g017400 OS=Sorghum bicolor GN=Sb04g017400 PE=4 SV=1)

HSP 1 Score: 316.235 bits (809), Expect = 1.336e-84
Identity = 146/162 (90.12%), Postives = 153/162 (94.44%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPSIYTSPGMYLVNHPVNLG QLAL IL+AG+LCIYINYDCDRQRQEFRRTNGK SVWGKAPSKI ASY TT GETK SLLLTSGWWGL+RHFHYVPEIL+AFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRC SKYGKYWK YC++VPYR+IPGIY
Sbjct:  293 VPSIYTSPGMYLVNHPVNLGPQLALSILLAGMLCIYINYDCDRQRQEFRRTNGKCSVWGKAPSKIVASYQTTKGETKTSLLLTSGWWGLSRHFHYVPEILSAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCSSKYGKYWKTYCNKVPYRVIPGIY 454

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BLAST of GR393338 vs. TrEMBL
Match: B4FWK5_MAIZE (7-dehydrocholesterol reductase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 316.235 bits (809), Expect = 1.336e-84
Identity = 145/162 (89.51%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPSIYTSPGMYLVNHP+NLG QLAL IL+AG+LCIYINYDCDRQRQEFRRTNGK SVWGKAPSKI ASY TT GETK SLLLTSGWWGL+RHFHYVPEIL+AFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRC SKYGKYWK+YC++VPYR+IPGIY
Sbjct:  289 VPSIYTSPGMYLVNHPLNLGPQLALSILLAGMLCIYINYDCDRQRQEFRRTNGKCSVWGKAPSKIVASYQTTKGETKTSLLLTSGWWGLSRHFHYVPEILSAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCSSKYGKYWKIYCNKVPYRVIPGIY 450

[Close]

BLAST of GR393338 vs. TrEMBL
Match: D7SS03_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_54.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029543001 PE=4 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.745e-84
Identity = 146/162 (90.12%), Postives = 152/162 (93.83%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPSIYTSPGMYLVNHPVNLG QLA++IL AGI CIYINYDCDRQRQEFRRTNGK  VWGKAPSKI ASYTTTSGETK SLLLTSGWWGL+RHFHYVPEILAAFFWTVPALF HFLPYFYV+FLTILLFDRAKRDDDRCRSKYGKYWK YC++V YRIIPGIY
Sbjct:  257 VPSIYTSPGMYLVNHPVNLGTQLAIYILAAGITCIYINYDCDRQRQEFRRTNGKCLVWGKAPSKIVASYTTTSGETKSSLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFGHFLPYFYVLFLTILLFDRAKRDDDRCRSKYGKYWKKYCEKVRYRIIPGIY 418

[Close]

BLAST of GR393338 vs. TrEMBL
Match: D7KFZ6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474170 PE=4 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 8.661e-84
Identity = 145/162 (89.51%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPV LG QLA++ILVAGILCIYINYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSGETK SLLLTSGWWGLARHFHYVPEIL+AFFWTVPALF++FL YFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYC++V YRIIPGIY
Sbjct:  271 VPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432

[Close]

BLAST of GR393338 vs. TrEMBL
Match: Q6K4V9_ORYSJ (Os02g0465400 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0035A24.50 PE=4 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 1.131e-83
Identity = 144/162 (88.89%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPSIYTSPGMYLVNHPVNLG QLAL IL+AGILCIYINYDCDRQRQEFRRTNGK S+WGKAPSKI ASY TT+GETK SLLLTSGWWGL+RHFHYVPEIL+AFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRC SKYGKYWK+YC++VP R+IPGIY
Sbjct:  289 VPSIYTSPGMYLVNHPVNLGPQLALSILLAGILCIYINYDCDRQRQEFRRTNGKCSIWGKAPSKIVASYQTTNGETKSSLLLTSGWWGLSRHFHYVPEILSAFFWTVPALFDHFLPYFYVIFLTILLFDRAKRDDDRCSSKYGKYWKMYCNKVPCRVIPGIY 450

[Close]

BLAST of GR393338 vs. TAIR peptide
Match: AT1G50430.2 (| Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol biosynthesis ERG4/ERG24 family | chr1:18682175-18685555 REVERSE LENGTH=431)

HSP 1 Score: 312.768 bits (800), Expect = 1.040e-85
Identity = 144/162 (88.89%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPV LG QLA++ILVAGILCIYINYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSGETK SLLLTSGWWGLARHFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYC++V YRIIPGIY
Sbjct:  270 VPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 431

[Close]

BLAST of GR393338 vs. TAIR peptide
Match: AT1G50430.1 (| Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol biosynthesis ERG4/ERG24 family | chr1:18682175-18685555 REVERSE LENGTH=432)

HSP 1 Score: 312.768 bits (800), Expect = 1.040e-85
Identity = 144/162 (88.89%), Postives = 154/162 (95.06%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            VPS+YTSPGMYLVNHPV LG QLA++ILVAGILCIYINYDCDRQRQEFRRTNGK  VWG+APSKI ASYTTTSGETK SLLLTSGWWGLARHFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYC++V YRIIPGIY
Sbjct:  271 VPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432

[Close]

BLAST of GR393338 vs. TAIR peptide
Match: AT3G52940.1 (| Symbols: FK, HYD2, ELL1 | Ergosterol biosynthesis ERG4/ERG24 family | chr3:19630369-19633112 REVERSE LENGTH=369)

HSP 1 Score: 94.3597 bits (233), Expect = 5.816e-20
Identity = 49/162 (30.25%), Postives = 88/162 (54.32%), Query Frame = -2

Query:  165 VPSIYTSPGMYLVNHPVNLGIQLALFILVAGILCIYINYDCDRQRQEFRRTNGKGSVWGKAPSKIEASYTTTSGETKRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDRVPYRIIPGIY 650
            +P  ++  G +L+++ V L +   +   +  ++   +    ++Q+  F++ N K  +WGK P  +               LL SG+WG+ARH +Y+ +++ A  +++P   +  +PYFY I+L ILL  R +RD+ RC  KY + W  Y   VP+RI+P +Y
Sbjct:  221 IPFTFSIQGWWLLHNKVELTVPAIVVNCLVFLIGYMVFRGANKQKHIFKK-NPKTPIWGKPPVVVGGK------------LLVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYLRLVPWRILPYVY 369

The following BLAST results are available for this feature:

BLAST of GR393338 vs. SwissProt
BLAST of GR393338 vs. TrEMBL
BLAST of GR393338 vs. TAIR peptide

BLAST of GR393338 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ST7R_ARATH	1.018e-84	88.89	7-dehydrocholesterol reductase OS=Arabidopsis thal...	[more]
DHCR7_XENTR	4.476e-40	45.68	7-dehydrocholesterol reductase OS=Xenopus tropical...	[more]
DHCR7_XENLA	8.441e-39	44.44	7-dehydrocholesterol reductase OS=Xenopus laevis G...	[more]
DHCR7_RAT	1.440e-38	45.68	7-dehydrocholesterol reductase OS=Rattus norvegicu...	[more]
DHCR7_BOVIN	1.440e-38	45.06	7-dehydrocholesterol reductase OS=Bos taurus GN=DH...	[more]
DHCR7_MOUSE	1.880e-38	45.68	7-dehydrocholesterol reductase OS=Mus musculus GN=...	[more]
DHCR7_HUMAN	1.880e-38	44.44	7-dehydrocholesterol reductase OS=Homo sapiens GN=...	[more]
DHCR7_DANRE	3.921e-36	42.59	7-dehydrocholesterol reductase OS=Danio rerio GN=d...	[more]
LBR_RAT	2.462e-30	40.74	Lamin-B receptor OS=Rattus norvegicus GN=Lbr PE=1 ...	[more]
LBR_MOUSE	4.199e-30	40.12	Lamin-B receptor OS=Mus musculus GN=Lbr PE=1 SV=2	[more]

BLAST of GR393338 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TIU6_SOYBN	3.631e-90	95.06	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q2QCX7_GOSHI	2.203e-87	90.74	Sterol delta-7 reductase DWF5 OS=Gossypium hirsutu...	[more]
Q6S5A3_TROMA	2.435e-86	91.36	Sterol delta-7 reductase OS=Tropaeolum majus PE=2 ...	[more]
B9HV17_POPTR	7.834e-85	89.51	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PC70_POPTR	7.834e-85	89.51	Putative uncharacterized protein OS=Populus tricho...	[more]
C5Y133_SORBI	1.336e-84	90.12	Putative uncharacterized protein Sb04g017400 OS=So...	[more]
B4FWK5_MAIZE	1.336e-84	89.51	7-dehydrocholesterol reductase OS=Zea mays PE=2 SV...	[more]
D7SS03_VITVI	1.745e-84	90.12	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KFZ6_ARALY	8.661e-84	89.51	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q6K4V9_ORYSJ	1.131e-83	88.89	Os02g0465400 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of GR393338 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G50430.2	1.040e-85	88.89	\| Symbols: DWF5, PA, LE, ST7R, 7RED \| Ergosterol b...	[more]
AT1G50430.1	1.040e-85	88.89	\| Symbols: DWF5, PA, LE, ST7R, 7RED \| Ergosterol b...	[more]
AT3G52940.1	5.816e-20	30.25	\| Symbols: FK, HYD2, ELL1 \| Ergosterol biosynthesi...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 27..53 score
None	No IPR available	PANTHER	PTHR21257	STEROL REDUCTASE/LAMIN B RECEPTOR	coord: 1..162 score: 1.5e
None	No IPR available	PANTHER	PTHR21257:SF16	7-DEHYDROCHOLESTEROL REDUCTASE	coord: 1..162 score: 1.5e
IPR001171	Ergosterol biosynthesis ERG4/ERG24	PFAM	PF01222	ERG4_ERG24	coord: 1..162 score: 2
IPR018083	Sterol reductase, conserved site	PROSITE	PS01018	STEROL_REDUCT_2	coord: 126..149 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR393338 ID=GR393338; Name=GR393338; organism=Cicer arietinum; type=EST; length=651bp
ATGTCATAACAATAGCATAAAAAATAGGGTTCAACAATAACTGAGTTATT
TCTTACACCTATAGTCTACACCATTGTATCTTAAGTACACCAAGCAGAGG
CAGAAAGAAGAGGGGAAAAACTAAACATGGCTCCAATTGAAGGATTCAAG
ACAAGTCATCATCAATATATTCCTGGAATGATTCTGTAAGGTACCCTGTC
GCAATAAAGTTTCCAGTACTTGCCATACTTGGATCTGCATCGATCATCAT
CTCTTTTTGCTCGATCAAAAAGAAGGATGGTAAGAAATATCACGTAAAAG
TAAGGCAAAAAATGATTAAAAAGAGCTGGGACTGTCCAGAAAAATGCAGC
TAGTATTTCGGGGACATAATGAAAATGACGAGCTAATCCCCACCATCCAG
ATGTTAAAAGAAGGCTTCTTTTTGTTTCCCCAGAAGTAGTAGTATATGAG
GCCTCTATCTTCGATGGAGCTTTTCCCCATACCGAGCCTTTTCCATTTGT
CCTACGAAACTCTTGCCTCTGCCTGTCACAATCATAGTTGATGTATATGC
ACAGAATGCCTGCCACTAAAATAAAGAGCGCTAGCTGGATGCCAAGATTT
ACTGGATGGTTGACAAGGTACATTCCAGGAGATGTATAAATAGATGGAAC
C

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR001171	ERG24_DHCR-like
IPR018083	Sterol_reductase_CS

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