GR390763

EST Overview


Name	GR390763
Unique Name	GR390763
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	287

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR390763 vs. SwissProt
Match: RL27A_SCHPO (60S ribosomal protein L27-A OS=Schizosaccharomyces pombe GN=rpl27a PE=2 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 2.100e-13
Identity = 33/56 (58.93%), Postives = 41/56 (73.21%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRY  EL+ LKG ++ DTFKE +QR  A+KTVKK   E+Y SGK+ W FTP +F
Sbjct:   81 MPTRYALELDNLKGLITADTFKEPTQRSAARKTVKKTFEEKYQSGKSAWFFTPLRF 136

[Close]

BLAST of GR390763 vs. SwissProt
Match: RL27B_SCHPO (60S ribosomal protein L27-B OS=Schizosaccharomyces pombe GN=rpl27b PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.755e-12
Identity = 32/56 (57.14%), Postives = 38/56 (67.86%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRY  EL+ LKG V+  TF E SQR  AKKTVK    E+Y +GK+ W FTP +F
Sbjct:   81 MPTRYALELDNLKGLVTPTTFSEPSQRSAAKKTVKNTFEEKYQTGKSAWFFTPLRF 136

[Close]

BLAST of GR390763 vs. SwissProt
Match: RL27B_YEAST (60S ribosomal protein L27-B OS=Saccharomyces cerevisiae GN=RPL27B PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.152e-11
Identity = 29/56 (51.79%), Postives = 41/56 (73.21%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            +PTRYT ++E  K  VS +TF++ SQRE+AKK VKK   ER+ +GKN+W F+  +F
Sbjct:   81 LPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136

[Close]

BLAST of GR390763 vs. SwissProt
Match: RL27A_YEAST (60S ribosomal protein L27-A OS=Saccharomyces cerevisiae GN=RPL27A PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.152e-11
Identity = 29/56 (51.79%), Postives = 41/56 (73.21%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            +PTRYT ++E  K  VS +TF++ SQRE+AKK VKK   ER+ +GKN+W F+  +F
Sbjct:   81 LPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136

[Close]

BLAST of GR390763 vs. SwissProt
Match: RL27_CANAL (60S ribosomal protein L27 OS=Candida albicans GN=RPL27 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.841e-10
Identity = 27/56 (48.21%), Postives = 39/56 (69.64%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRY+ ++E  K AV+++  +E SQRE+AKK VKK   E++ +GKN+W F    F
Sbjct:   81 MPTRYSLDVESFKSAVTSEALEEPSQREEAKKVVKKAFEEKHQAGKNKWFFQKLHF 136

[Close]

BLAST of GR390763 vs. TrEMBL
Match: C7YPQ9_NECH7 (Predicted protein OS=Nectria haematococca (strain 77-13-4 / FGSC 9596 / MPVI) GN=NECHADRAFT_59945 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.135e-21
Identity = 49/56 (87.50%), Postives = 51/56 (91.07%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG++SNDTFKEVSQREDAKKTVKKVL ERY SGKNRW FTP KF
Sbjct:   80 MPTRYTLELEGLKGSISNDTFKEVSQREDAKKTVKKVLEERYTSGKNRWFFTPLKF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: B6Q3I2_PENMQ (60S ribosomal protein L27 OS=Penicillium marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) GN=PMAA_019840 PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.328e-20
Identity = 48/56 (85.71%), Postives = 49/56 (87.50%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG VSNDTFKEVSQREDAKKTVKK L ERY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGVVSNDTFKEVSQREDAKKTVKKALEERYTSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: A4R2B6_MAGGR (Ribosomal protein L27 OS=Magnaporthe grisea GN=MGG_06693 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.328e-20
Identity = 48/56 (85.71%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG+V+NDTFKEVSQREDAKK VKKVL ERY SGKNRW FTP KF
Sbjct:   80 MPTRYTLELEGLKGSVTNDTFKEVSQREDAKKNVKKVLEERYTSGKNRWFFTPLKF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: B8M6M8_TALSN (60S ribosomal protein L27 OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) GN=TSTA_027810 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.347e-20
Identity = 47/56 (83.93%), Postives = 49/56 (87.50%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG +SNDTFKEVSQREDAKKTVKK L ERY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGVISNDTFKEVSQREDAKKTVKKALEERYQSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: C9SCT3_VERA1 (60S ribosomal protein L27-B OS=Verticillium albo-atrum (strain VaMs.102) GN=VDBG_03007 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.677e-20
Identity = 48/56 (85.71%), Postives = 49/56 (87.50%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG VSNDTFKE SQRED+KKTVKKVL ERY SGKNRW FTP KF
Sbjct:   80 MPTRYTLELEGLKGTVSNDTFKEPSQREDSKKTVKKVLEERYTSGKNRWFFTPLKF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: C5FDL3_NANOT (60S ribosomal protein L27 OS=Nannizzia otae (strain CBS 113480) GN=MCYG_00697 PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.684e-20
Identity = 46/56 (82.14%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKGAV+NDTFKEVSQRE+AKKT+KK L ERY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGAVTNDTFKEVSQREEAKKTIKKALEERYTSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: Q2UND2_ASPOR (60S ribosomal protein L27 OS=Aspergillus oryzae GN=AO090001000413 PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.265e-19
Identity = 46/56 (82.14%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG+V+NDTFKEVSQREDAKKTVKK L +RY SGKNRW FTP +F
Sbjct:   77 MPTRYTLELEGLKGSVTNDTFKEVSQREDAKKTVKKALEDRYTSGKNRWFFTPLRF 132

[Close]

BLAST of GR390763 vs. TrEMBL
Match: B8NMV9_ASPFN (60S ribosomal protein L27 OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) GN=AFLA_127220 PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.265e-19
Identity = 46/56 (82.14%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG+V+NDTFKEVSQREDAKKTVKK L +RY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGSVTNDTFKEVSQREDAKKTVKKALEDRYTSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: C1GTU4_PARBA (60S ribosomal protein L27 OS=Paracoccidioides brasiliensis (strain ATCC MYA-826 / Pb01) GN=PAAG_01939 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.652e-19
Identity = 46/56 (82.14%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKGA++NDTFKEVSQRE+AKKTVKK L ERY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGAITNDTFKEVSQREEAKKTVKKSLEERYNSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TrEMBL
Match: Q872T5_NEUCR (60S ribosomal protein L27 OS=Neurospora crassa GN=B23G1.190 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.157e-19
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT ELEGLKG+VS +TFKEVSQREDAKKTVKKVL ERY SGKNRW FTP +F
Sbjct:   80 MPTRYTLELEGLKGSVSAETFKEVSQREDAKKTVKKVLEERYTSGKNRWFFTPLRF 135

[Close]

BLAST of GR390763 vs. TAIR peptide
Match: AT2G32220.1 (| Symbols: | Ribosomal L27e protein family | chr2:13678945-13679352 FORWARD LENGTH=135)

HSP 1 Score: 49.6766 bits (117), Expect = 4.513e-7
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = -1

Query:  120 MPTRYTXELEGLKGAVSNDTFKEVSQREDAKKTVKKVLXERYXSGKNRWXFTPXKF 287
            MPTRYT +L+ LK  VS D      ++  A K  K    ER+ +GKNRW FT  +F
Sbjct:   81 MPTRYTLDLD-LKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135

The following BLAST results are available for this feature:

BLAST of GR390763 vs. SwissProt
BLAST of GR390763 vs. TrEMBL
BLAST of GR390763 vs. TAIR peptide

BLAST of GR390763 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
RL27A_SCHPO	2.100e-13	58.93	60S ribosomal protein L27-A OS=Schizosaccharomyces...	[more]
RL27B_SCHPO	6.755e-12	57.14	60S ribosomal protein L27-B OS=Schizosaccharomyces...	[more]
RL27B_YEAST	1.152e-11	51.79	60S ribosomal protein L27-B OS=Saccharomyces cerev...	[more]
RL27A_YEAST	1.152e-11	51.79	60S ribosomal protein L27-A OS=Saccharomyces cerev...	[more]
RL27_CANAL	4.841e-10	48.21	60S ribosomal protein L27 OS=Candida albicans GN=R...	[more]

BLAST of GR390763 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C7YPQ9_NECH7	5.135e-21	87.50	Predicted protein OS=Nectria haematococca (strain ...	[more]
B6Q3I2_PENMQ	3.328e-20	85.71	60S ribosomal protein L27 OS=Penicillium marneffei...	[more]
A4R2B6_MAGGR	3.328e-20	85.71	Ribosomal protein L27 OS=Magnaporthe grisea GN=MGG...	[more]
B8M6M8_TALSN	4.347e-20	83.93	60S ribosomal protein L27 OS=Talaromyces stipitatu...	[more]
C9SCT3_VERA1	5.677e-20	85.71	60S ribosomal protein L27-B OS=Verticillium albo-a...	[more]
C5FDL3_NANOT	9.684e-20	82.14	60S ribosomal protein L27 OS=Nannizzia otae (strai...	[more]
Q2UND2_ASPOR	1.265e-19	82.14	60S ribosomal protein L27 OS=Aspergillus oryzae GN...	[more]
B8NMV9_ASPFN	1.265e-19	82.14	60S ribosomal protein L27 OS=Aspergillus flavus (s...	[more]
C1GTU4_PARBA	1.652e-19	82.14	60S ribosomal protein L27 OS=Paracoccidioides bras...	[more]
Q872T5_NEUCR	2.157e-19	83.93	60S ribosomal protein L27 OS=Neurospora crassa GN=...	[more]

BLAST of GR390763 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G32220.1	4.513e-7	44.64	\| Symbols: \| Ribosomal L27e protein family \| chr2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 51..65 score: NAcoord: 71..81 score
IPR001141	Ribosomal protein L27e	PANTHER	PTHR10497	60S RIBOSOMAL PROTEIN L27E	coord: 1..56 score: 1.3
IPR001141	Ribosomal protein L27e	PFAM	PF01777	Ribosomal_L27e	coord: 1..56 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR390763 ID=GR390763; Name=GR390763; organism=Cicer arietinum; type=EST; length=287bp
TTTTTTTGCNTTAANAAACTTCATATTGAGCCCATAAAAACCGGCGTTCC
TTAATGNCATCGATGCTCTGTACATCCCGCCAACTCCCTAACCGTGATGG
ANAAACGATTCCTTTTTTAAAACTTGANAGGGGTGAANAACCATCGGTTC
TTGCCGCTGGNGTATCGCTCCNCGAGCACCTTCTTGACAGTCTTCTTGGC
ATCCTCGCGCTGGGAAACCTCCTTGAANGTGTCGTTAGANACGGCGCCCT
TGAGGCCCTCNAGCTCCANGGTGTANCGAGTGGGCAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR001141	Ribosomal_eL27

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