GR407327

EST Overview


Name	GR407327
Unique Name	GR407327
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	437

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR407327 vs. SwissProt
Match: TDIF2_HUMAN (Deoxynucleotidyltransferase terminal-interacting protein 2 OS=Homo sapiens GN=DNTTIP2 PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 2.679e-9
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  208 FPKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAK 435
            FPKYFQ+GT+ +   D++  R+ KK+RK T+ EE L D     Y +RK  EI   +           G+ FRK+ K
Sbjct:  684 FPKYFQIGTIVDNPADFYHSRIPKKQRKRTIVEELLADSEFRRYNRRKYSEIMAEKAANA------AGKKFRKKKK 753

[Close]

BLAST of GR407327 vs. SwissProt
Match: TDIF2_BOVIN (Deoxynucleotidyltransferase terminal-interacting protein 2 OS=Bos taurus GN=DNTTIP2 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.498e-9
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = -3

Query:  208 FPKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAK 435
            FPKYFQ+GT+ +   D++  R+ KK+RK T+ EE L D     Y +RK  EI   +           G+ FRK+ K
Sbjct:  694 FPKYFQIGTIVDNPADFYHSRVPKKQRKRTIVEELLADSEFRRYNRRKYSEIMAEKAANA------AGKKFRKKKK 763

[Close]

BLAST of GR407327 vs. SwissProt
Match: TDIF2_MOUSE (Deoxynucleotidyltransferase terminal-interacting protein 2 OS=Mus musculus GN=Dnttip2 PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.967e-9
Identity = 28/76 (36.84%), Postives = 42/76 (55.26%), Query Frame = -3

Query:  208 FPKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAK 435
            FPKYFQVGT+ +   D++  R+ KK+RK T+ EE L D     + +RK  EI   +          +G+ F+K+ K
Sbjct:  686 FPKYFQVGTIVDNPADFYHSRIPKKQRKKTIVEELLADSEFRRFNRRKYSEIMAEKAANA------EGKKFKKKKK 755

[Close]

BLAST of GR407327 vs. SwissProt
Match: FCF2_SCHPO (rRNA-processing protein fcf2 OS=Schizosaccharomyces pombe GN=fcf2 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.294e-7
Identity = 23/64 (35.94%), Postives = 39/64 (60.94%), Query Frame = -3

Query:  241 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWK 432
            PKYFQVG++ E   D++  R+  +ERK T+ +E L D    +Y K+K  E+++ +  G   ++K
Sbjct:  155 PKYFQVGSIVEGPQDFYSSRIPTRERKETIVDELLHDSERRSYFKKKYLELQKSKMSGRKGQYK 218

[Close]

BLAST of GR407327 vs. TrEMBL
Match: B7FIY2_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.747e-27
Identity = 57/80 (71.25%), Postives = 68/80 (85.00%), Query Frame = -3

Query:  193 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRKY 432
            PKYFQVGT+  + +DY+ GRL  +ERK ++A+E L DQN +AYRKRKVREIEEHRRP GNEKWKIQG+S RKRAKE+RKY
Sbjct:  121 PKYFQVGTMVGSPLDYYSGRLTNRERKESIADELLSDQNFSAYRKRKVREIEEHRRPAGNEKWKIQGKSTRKRAKERRKY 200

[Close]

BLAST of GR407327 vs. TrEMBL
Match: C6TNR6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.077e-26
Identity = 59/80 (73.75%), Postives = 66/80 (82.50%), Query Frame = -3

Query:  193 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRKY 432
            PKYFQVGTV +  +D+F G L KKERKATLA+E L DQNLAAYRKRKVREIEE  RP GNEKWKI+G + RKRAKE+R Y
Sbjct:  120 PKYFQVGTVVDFPLDFFSGGLTKKERKATLADELLSDQNLAAYRKRKVREIEEQNRPAGNEKWKIKGSNSRKRAKERRIY 199

[Close]

BLAST of GR407327 vs. TrEMBL
Match: A9PHG8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820943 PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 6.854e-26
Identity = 55/78 (70.51%), Postives = 66/78 (84.62%), Query Frame = -3

Query:  199 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKR 432
            PKYFQVGTV E++ D++ GRL KKERKAT+A+E L+DQ   AYRKRKVREIEE  RPGGN+KWKI+G+  RKRAKE+R
Sbjct:  120 PKYFQVGTVVESATDFYSGRLTKKERKATIADELLYDQTFRAYRKRKVREIEEKNRPGGNDKWKIKGKQSRKRAKERR 197

[Close]

BLAST of GR407327 vs. TrEMBL
Match: B9S002_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1003610 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 2.605e-25
Identity = 57/78 (73.08%), Postives = 65/78 (83.33%), Query Frame = -3

Query:  199 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKR 432
            PKYFQVGTV E++ DYF GRL KKERK TLAEE L D+ LAAYRKRKVREIEE   PGGNE+WKI+G+  RKRAK++R
Sbjct:  122 PKYFQVGTVVESATDYFSGRLTKKERKPTLAEEILSDRTLAAYRKRKVREIEEQDGPGGNERWKIKGRHSRKRAKDRR 199

[Close]

BLAST of GR407327 vs. TrEMBL
Match: B9HXA3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_565466 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 9.898e-25
Identity = 53/78 (67.95%), Postives = 65/78 (83.33%), Query Frame = -3

Query:  199 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKR 432
            PKYFQVGTV E++ D++ GRL KKERK T+A+E L+DQ  +AYRKRKVREIEE  RPGGN+KWKI+G+   KRAKE+R
Sbjct:  112 PKYFQVGTVVESATDFYSGRLTKKERKVTIADELLYDQTFSAYRKRKVREIEEKNRPGGNDKWKIKGKQSWKRAKERR 189

[Close]

BLAST of GR407327 vs. TrEMBL
Match: A2X988_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_08805 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.303e-20
Identity = 47/79 (59.49%), Postives = 61/79 (77.22%), Query Frame = -3

Query:  196 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRK 432
            PKYFQVGTV E + ++F  RL K+ERK TL +E L DQ+L  YR RKVREI+E+R PGGN+KW+ +G+   KRAK++RK
Sbjct:  117 PKYFQVGTVIEPASEFFSSRLTKRERKTTLVDELLSDQHLKNYRMRKVREIQENRTPGGNQKWRNKGKKTLKRAKDRRK 195

[Close]

BLAST of GR407327 vs. TrEMBL
Match: C5XZD1_SORBI (Putative uncharacterized protein Sb04g029010 OS=Sorghum bicolor GN=Sb04g029010 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.620e-20
Identity = 47/79 (59.49%), Postives = 61/79 (77.22%), Query Frame = -3

Query:  196 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRK 432
            PKYFQVGTV E + +++  RL  +ERKATL +E L DQ+L +YR RKVREI+E R PGGN+KWK +G+   KRAK++RK
Sbjct:  119 PKYFQVGTVVEPASEFYSSRLKNRERKATLVDELLSDQSLKSYRMRKVREIQEIRTPGGNQKWKNKGKQTLKRAKDRRK 197

[Close]

BLAST of GR407327 vs. TrEMBL
Match: B6TJ58_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.620e-20
Identity = 47/79 (59.49%), Postives = 61/79 (77.22%), Query Frame = -3

Query:  196 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRK 432
            PKYFQVGTV E + +++  RL  +ERKATL +E L DQ+L +YR RKVREI+E R PGGN+KWK +G+   KRAK++RK
Sbjct:  119 PKYFQVGTVVEPASEFYSSRLKNRERKATLVDELLSDQSLKSYRMRKVREIQEIRTPGGNQKWKNKGKKTLKRAKDRRK 197

[Close]

BLAST of GR407327 vs. TrEMBL
Match: B6TCJ0_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.620e-20
Identity = 47/79 (59.49%), Postives = 61/79 (77.22%), Query Frame = -3

Query:  196 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRK 432
            PKYFQVGTV E + +++  RL  +ERKATL +E L DQ+L +YR RKVREI+E R PGGN+KWK +G+   KRAK++RK
Sbjct:  119 PKYFQVGTVVEPASEFYSSRLKNRERKATLVDELLSDQSLKSYRMRKVREIQEIRTPGGNQKWKNKGKKTLKRAKDRRK 197

[Close]

BLAST of GR407327 vs. TrEMBL
Match: Q6YWQ2_ORYSJ (Acidic 82 kDa protein-like OS=Oryza sativa subsp. japonica GN=OSJNBa0072H09.39 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.789e-19
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = -3

Query:  196 PKYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKRK 432
            PKYFQVGTV E + ++F  RL K+ERK TL +E L DQ+L  YR RKVREI+E R PGG++KW+ +G+   KRAK++RK
Sbjct:  117 PKYFQVGTVIEPASEFFSSRLTKRERKTTLVDELLSDQHLKNYRMRKVREIQESRTPGGSQKWRNKGKKTLKRAKDRRK 195

[Close]

BLAST of GR407327 vs. TAIR peptide
Match: AT1G54770.1 (| Symbols: | Fcf2 pre-rRNA processing protein | chr1:20434803-20435815 FORWARD LENGTH=189)

HSP 1 Score: 76.6406 bits (187), Expect = 5.737e-15
Identity = 42/77 (54.55%), Postives = 51/77 (66.23%), Query Frame = -3

Query:  199 KYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEKR 429
            KYFQ+GTV E + + F+GRL KK RKATLA+E + D   A YRKRKVREIEE  R   N+KW  +G    K  K +R
Sbjct:  114 KYFQIGTVIEPAEE-FYGRLTKKNRKATLADELVSDPKTALYRKRKVREIEEKSRAVTNKKWNKKGNQ-SKNTKPRR 188

[Close]

BLAST of GR407327 vs. TAIR peptide
Match: AT5G30495.1 (| Symbols: | Fcf2 pre-rRNA processing protein | chr5:11380190-11381391 FORWARD LENGTH=196)

HSP 1 Score: 76.2554 bits (186), Expect = 7.493e-15
Identity = 38/76 (50.00%), Postives = 52/76 (68.42%), Query Frame = -3

Query:  202 KYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEK 429
            KYFQ+GTV E + + F+GRL KK RKATLA+E + D  ++ YRKRKV+EIEE  R   N+KWK +G     + + +
Sbjct:  121 KYFQIGTVIEPAEE-FYGRLTKKNRKATLADELVSDPKVSQYRKRKVKEIEEKSRAVTNKKWKKKGNQTTNKKQRR 195

[Close]

BLAST of GR407327 vs. TAIR peptide
Match: AT5G30495.2 (| Symbols: | Fcf2 pre-rRNA processing protein | chr5:11380190-11381391 FORWARD LENGTH=196)

HSP 1 Score: 76.2554 bits (186), Expect = 7.493e-15
Identity = 38/76 (50.00%), Postives = 52/76 (68.42%), Query Frame = -3

Query:  202 KYFQVGTVEETSVDYFFGRLNKKERKATLAEEFLFDQNLAAYRKRKVREIEEHRRPGGNEKWKIQGQSFRKRAKEK 429
            KYFQ+GTV E + + F+GRL KK RKATLA+E + D  ++ YRKRKV+EIEE  R   N+KWK +G     + + +
Sbjct:  121 KYFQIGTVIEPAEE-FYGRLTKKNRKATLADELVSDPKVSQYRKRKVKEIEEKSRAVTNKKWKKKGNQTTNKKQRR 195

The following BLAST results are available for this feature:

BLAST of GR407327 vs. SwissProt
BLAST of GR407327 vs. TrEMBL
BLAST of GR407327 vs. TAIR peptide

BLAST of GR407327 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
TDIF2_HUMAN	2.679e-9	38.16	Deoxynucleotidyltransferase terminal-interacting p...	[more]
TDIF2_BOVIN	3.498e-9	38.16	Deoxynucleotidyltransferase terminal-interacting p...	[more]
TDIF2_MOUSE	5.967e-9	36.84	Deoxynucleotidyltransferase terminal-interacting p...	[more]
FCF2_SCHPO	7.294e-7	35.94	rRNA-processing protein fcf2 OS=Schizosaccharomyce...	[more]

BLAST of GR407327 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FIY2_MEDTR	4.747e-27	71.25	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TNR6_SOYBN	3.077e-26	73.75	Putative uncharacterized protein OS=Glycine max PE...	[more]
A9PHG8_POPTR	6.854e-26	70.51	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S002_RICCO	2.605e-25	73.08	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9HXA3_POPTR	9.898e-25	67.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A2X988_ORYSI	4.303e-20	59.49	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5XZD1_SORBI	5.620e-20	59.49	Putative uncharacterized protein Sb04g029010 OS=So...	[more]
B6TJ58_MAIZE	5.620e-20	59.49	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TCJ0_MAIZE	5.620e-20	59.49	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q6YWQ2_ORYSJ	2.789e-19	58.23	Acidic 82 kDa protein-like OS=Oryza sativa subsp. ...	[more]

BLAST of GR407327 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G54770.1	5.737e-15	54.55	\| Symbols: \| Fcf2 pre-rRNA processing protein \| c...	[more]
AT5G30495.1	7.493e-15	50.00	\| Symbols: \| Fcf2 pre-rRNA processing protein \| c...	[more]
AT5G30495.2	7.493e-15	50.00	\| Symbols: \| Fcf2 pre-rRNA processing protein \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 80..91 score
None	No IPR available	PANTHER	PTHR21686	UNCHARACTERIZED	coord: 3..79 score: 7.4
None	No IPR available	PANTHER	PTHR21686:SF3	UNCHARACTERIZED	coord: 3..79 score: 7.4
IPR014810	Fcf2 pre-rRNA processing	PFAM	PF08698	Fcf2	coord: 2..48 score: 6.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR407327 ID=GR407327; Name=GR407327; organism=Cicer arietinum; type=EST; length=437bp
GTACTACAAAACATGAGACTACATTGACCCACTTGATATAAATCATATAA
CAACCAGTTGGTTTTATAGTTATTTTTACATGAACTCAATAATTGTGAAA
TGTTGAATCATTAATTTCTTGGCATGTTAACAAGATTGAATGTAATGAAC
TTCAAATGTCACGGGTTAACTTCAAAAAGTGTGTAAAATTCAGTACTTCC
TCTTTTCTTTTGCGCGCTTACGAAAACTCTGACCTTGGATCTTCCACTTT
TCATTCCCGCCTGGTCGTCTATGCTCTTCAATTTCTCGAACCTTCCGCTT
CCTGTAAGCTGCAAGGTTTTGATCAAAAAGGAACTCTTCTGCAAGTGTTG
CTTTCCTCTCTTTCTTATTTAATCTTCCAAAAAAATAGTCCACTGAAGTT
TCTTCAACTGTTCCCACCTGAAAATACTTCGGAAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR014810	Fcf2_C

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