GR913813

EST Overview


Name	GR913813
Unique Name	GR913813
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	459

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR913813 vs. TrEMBL
Match: D7SL85_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025310001 PE=4 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 2.241e-61
Identity = 110/140 (78.57%), Postives = 126/140 (90.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SA+++ L+ CQEK+GTGYLSAFP E FDRFEAI+PVWAPYYTIHKILAGLLDQYT AGN QALKM+TWMV++FY RV NVI+ Y+L RH+ SLN+ETGGMND+LYRLYSITGD KHL+LAHLFDKP
Sbjct:  181 MWASTHNDTLKEKMSAVVSALATCQEKMGTGYLSAFPSELFDRFEAIKPVWAPYYTIHKILAGLLDQYTFAGNSQALKMMTWMVEHFYKRVQNVITMYSLERHWLSLNEETGGMNDVLYRLYSITGDQKHLVLAHLFDKP 320

[Close]

BLAST of GR913813 vs. TrEMBL
Match: B9GW92_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1074570 PE=4 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 4.992e-61
Identity = 110/140 (78.57%), Postives = 126/140 (90.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST N+TLK K+SA+++ LSACQ K+GTGYLSAFP E FDRFEAI+PVWAPYYTIHKILAGLLDQYT+A N QALKMV WMVDYFYNRV NVI+ Y++ RHY SLN+ETGGMND+LY+L+SITGD KHL+LAHLFDKP
Sbjct:  176 MWASTHNETLKKKMSAVVSALSACQVKMGTGYLSAFPSELFDRFEAIKPVWAPYYTIHKILAGLLDQYTLADNAQALKMVKWMVDYFYNRVRNVITNYSVERHYLSLNEETGGMNDVLYKLFSITGDPKHLVLAHLFDKP 315

[Close]

BLAST of GR913813 vs. TrEMBL
Match: B9GM82_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_814847 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.414e-60
Identity = 106/140 (75.71%), Postives = 126/140 (90.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST ND L+ ++SA+++ LS+CQEK+G+GYLSAFP E FDRFEAI+PVWAPYYTIHKILAGLLDQYT A N QALKMV WMVDYFYNRV NVI+ +++ RHY+SLN+ETGGMND+LY+L+SITGD KHL+LAHLFDKP
Sbjct:  176 MWASTHNDILEKQMSAVVSALSSCQEKMGSGYLSAFPSELFDRFEAIKPVWAPYYTIHKILAGLLDQYTFADNAQALKMVKWMVDYFYNRVRNVITNFSVERHYQSLNEETGGMNDVLYKLFSITGDPKHLVLAHLFDKP 315

[Close]

BLAST of GR913813 vs. TrEMBL
Match: Q9LXU4_ARATH (Putative uncharacterized protein At5g12950/T24H18_120 OS=Arabidopsis thaliana GN=T24H18_120 PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.606e-59
Identity = 109/140 (77.86%), Postives = 123/140 (87.86%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SAL++ LS CQ+K GTGYLSAFP  FFDRFEAI PVWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH++SLN+ETGGMND+LY+LYSITGDSK+LLLAHLFDKP
Sbjct:  181 MWASTHNDTLKEKMSALVSALSECQQKSGTGYLSAFPSSFFDRFEAITPVWAPYYTIHKILAGLVDQYKLAGNSQALKMATGMADYFYGRVRNVIRKYSVERHWQSLNEETGGMNDVLYQLYSITGDSKYLLLAHLFDKP 320

[Close]

BLAST of GR913813 vs. TrEMBL
Match: D7M550_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_909245 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.102e-59
Identity = 108/140 (77.14%), Postives = 123/140 (87.86%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SAL++ LS CQ+K GTGYLSAFP  FFDRFEAI PVWAPYYTIHKI+AGL+DQY +AGN QAL+M T M DYFY RV NVI KY++ RH++SLN+ETGGMNDILY+LYSITGDSK+LLLAHLFDKP
Sbjct:  180 MWASTHNDTLKEKMSALVSALSECQQKSGTGYLSAFPSSFFDRFEAITPVWAPYYTIHKIIAGLVDQYKLAGNSQALQMATGMADYFYGRVRNVIRKYSVERHWQSLNEETGGMNDILYQLYSITGDSKYLLLAHLFDKP 319

[Close]

BLAST of GR913813 vs. TrEMBL
Match: D7SL96_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025321001 PE=4 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 3.028e-58
Identity = 104/140 (74.29%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST N++LK K+SA++  L  CQ+K+GTGYLSAFP E FDRFEA++ VWAPYYTIHKILAGLLDQYT+ GN QALKMVTWMV+YFYNRV NVIS Y++ RH+ SLN+ETGGMND LY LY ITGD KH +LAHLFDKP
Sbjct:   49 MWASTHNESLKEKMSAVVCALGECQKKMGTGYLSAFPSELFDRFEALEEVWAPYYTIHKILAGLLDQYTLGGNAQALKMVTWMVEYFYNRVQNVISSYSIERHWLSLNEETGGMNDFLYNLYRITGDQKHFVLAHLFDKP 188

[Close]

BLAST of GR913813 vs. TrEMBL
Match: D7M4H7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_488069 PE=4 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 1.503e-57
Identity = 106/140 (75.71%), Postives = 121/140 (86.43%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SAL++ L+ CQ+K GTGYLSAFP  FFDRFEAI  VWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI+KY++ RHY+SLN+ETGGMND+LY+LYSIT DSK+L LAHLFDKP
Sbjct:  180 MWASTHNDTLKAKMSALVSALAECQQKSGTGYLSAFPSSFFDRFEAITHVWAPYYTIHKILAGLVDQYKLAGNIQALKMATGMADYFYGRVRNVITKYSVERHYQSLNEETGGMNDVLYQLYSITRDSKYLFLAHLFDKP 319

[Close]

BLAST of GR913813 vs. TrEMBL
Match: D7M552_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_350453 PE=4 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 2.835e-56
Identity = 105/140 (75.00%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SAL++ L+ CQ+K GTGYLSAFP  FFDRFEAI  VWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH+ SLN+ETGGMND+LY+LYSIT DSK+L LAHLFDKP
Sbjct:  180 MWASTHNDTLKTKMSALVSALAECQQKSGTGYLSAFPSSFFDRFEAITHVWAPYYTIHKILAGLVDQYKLAGNTQALKMATGMADYFYGRVQNVIRKYSVERHWLSLNEETGGMNDVLYQLYSITRDSKYLFLAHLFDKP 319

[Close]

BLAST of GR913813 vs. TrEMBL
Match: Q9LXU3_ARATH (Putative uncharacterized protein T24H18_130 OS=Arabidopsis thaliana GN=T24H18_130 PE=4 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.315e-56
Identity = 103/140 (73.57%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST N+TLK K++AL++ L+ CQ+K GTGYLSAFP  FFDRFEAI  VWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH+ SLN+ETGGMND+LY+LYSIT DSK+L LAHLFDKP
Sbjct:  180 MWASTHNETLKAKMTALVSALAECQQKYGTGYLSAFPSSFFDRFEAITHVWAPYYTIHKILAGLVDQYKLAGNTQALKMATGMADYFYGRVQNVIKKYSVERHWLSLNEETGGMNDVLYQLYSITRDSKYLFLAHLFDKP 319

[Close]

BLAST of GR913813 vs. TrEMBL
Match: Q84W43_ARATH (Putative uncharacterized protein At5g12960 OS=Arabidopsis thaliana GN=At5g12960 PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.315e-56
Identity = 103/140 (73.57%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST N+TLK K++AL++ L+ CQ+K GTGYLSAFP  FFDRFEAI  VWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH+ SLN+ETGGMND+LY+LYSIT DSK+L LAHLFDKP
Sbjct:  185 MWASTHNETLKAKMTALVSALAECQQKYGTGYLSAFPSSFFDRFEAITHVWAPYYTIHKILAGLVDQYKLAGNTQALKMATGMADYFYGRVQNVIKKYSVERHWLSLNEETGGMNDVLYQLYSITRDSKYLFLAHLFDKP 324

[Close]

BLAST of GR913813 vs. TAIR peptide
Match: AT5G12950.1 (| Symbols: | Putative glycosyl hydrolase of unknown function (DUF1680) | chr5:4093117-4096806 FORWARD LENGTH=861)

HSP 1 Score: 231.876 bits (590), Expect = 1.202e-61
Identity = 109/140 (77.86%), Postives = 123/140 (87.86%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST NDTLK K+SAL++ LS CQ+K GTGYLSAFP  FFDRFEAI PVWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH++SLN+ETGGMND+LY+LYSITGDSK+LLLAHLFDKP
Sbjct:  181 MWASTHNDTLKEKMSALVSALSECQQKSGTGYLSAFPSSFFDRFEAITPVWAPYYTIHKILAGLVDQYKLAGNSQALKMATGMADYFYGRVRNVIRKYSVERHWQSLNEETGGMNDVLYQLYSITGDSKYLLLAHLFDKP 320

[Close]

BLAST of GR913813 vs. TAIR peptide
Match: AT5G12960.1 (| Symbols: | Putative glycosyl hydrolase of unknown function (DUF1680) | chr5:4097788-4101518 FORWARD LENGTH=865)

HSP 1 Score: 219.935 bits (559), Expect = 4.725e-58
Identity = 103/140 (73.57%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   40 MWASTKNDTLKGKVSALIAGLSACQEKIGTGYLSAFPPEFFDRFEAIQPVWAPYYTIHKILAGLLDQYTVAGNPQALKMVTWMVDYFYNRVVNVISKYTLNRHYESLNDETGGMNDILYRLYSITGDSKHLLLAHLFDKP 459
            MWAST N+TLK K++AL++ L+ CQ+K GTGYLSAFP  FFDRFEAI  VWAPYYTIHKILAGL+DQY +AGN QALKM T M DYFY RV NVI KY++ RH+ SLN+ETGGMND+LY+LYSIT DSK+L LAHLFDKP
Sbjct:  185 MWASTHNETLKAKMTALVSALAECQQKYGTGYLSAFPSSFFDRFEAITHVWAPYYTIHKILAGLVDQYKLAGNTQALKMATGMADYFYGRVQNVIKKYSVERHWLSLNEETGGMNDVLYQLYSITRDSKYLFLAHLFDKP 324

The following BLAST results are available for this feature:

BLAST of GR913813 vs. TrEMBL
BLAST of GR913813 vs. TAIR peptide

BLAST of GR913813 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SL85_VITVI	2.241e-61	78.57	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GW92_POPTR	4.992e-61	78.57	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GM82_POPTR	9.414e-60	75.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9LXU4_ARATH	1.606e-59	77.86	Putative uncharacterized protein At5g12950/T24H18_...	[more]
D7M550_ARALY	6.102e-59	77.14	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7SL96_VITVI	3.028e-58	74.29	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7M4H7_ARALY	1.503e-57	75.71	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7M552_ARALY	2.835e-56	75.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9LXU3_ARATH	6.315e-56	73.57	Putative uncharacterized protein T24H18_130 OS=Ara...	[more]
Q84W43_ARATH	6.315e-56	73.57	Putative uncharacterized protein At5g12960 OS=Arab...	[more]

BLAST of GR913813 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G12950.1	1.202e-61	77.86	\| Symbols: \| Putative glycosyl hydrolase of unkno...	[more]
AT5G12960.1	4.725e-58	73.57	\| Symbols: \| Putative glycosyl hydrolase of unkno...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012878	Protein of unknown function DUF1680	PFAM	PF07944	DUF1680	coord: 10..150 score: 3.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR913813 ID=GR913813; Name=GR913813; organism=Cicer arietinum; type=EST; length=459bp
GATTAGCGTGGTCGCGGCCGAGGTCGGCCGAGGTCTCTAATGTGGGCGAG
CACAAAGAATGATACCCTAAAGGGGAAAGTTTCAGCTCTAATTGCCGGTC
TATCTGCCTGTCAGGAGAAAATTGGAACAGGATATCTCTCGGCATTTCCA
CCTGAGTTTTTCGACCGATTTGAAGCTATTCAACCTGTGTGGGCTCCCTA
TTACACAATTCACAAGATCTTGGCTGGCTTGTTGGATCAATATACTGTAG
CCGGAAACCCTCAAGCTCTCAAAATGGTGACATGGATGGTTGATTACTTT
TACAACCGAGTGGTGAATGTAATATCAAAGTACACTCTAAATAGACACTA
TGAATCACTGAATGACGAAACTGGAGGAATGAATGATATACTTTACAGAT
TATATAGCATAACGGGCGATTCAAAGCATCTATTGTTGGCGCACCTTTTC
GACAAGCCA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR012878	Beta-AFase-like_GH127_cat

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