GR391162

EST Overview


Name	GR391162
Unique Name	GR391162
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	628

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR391162 vs. SwissProt
Match: PR4B_TOBAC (Pathogenesis-related protein PR-4B OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.399e-11
Identity = 41/95 (43.16%), Postives = 46/95 (48.42%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            S Y L  PQNI+WD    S + AT+D + PLA      WTAF    G +G DSC  CL              IV QCS GGLD  VNV   L  N
Sbjct:   33 STYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKYGWTAFCGPAGPRGQDSCGRCLRVTNTGTGTQATVRIVDQCSNGGLDLDVNVFNQLDTN 127

[Close]

BLAST of GR391162 vs. SwissProt
Match: PR4A_TOBAC (Pathogenesis-related protein PR-4A OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.399e-11
Identity = 41/95 (43.16%), Postives = 46/95 (48.42%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            S Y L  PQNI+WD    S + AT+D + PLA      WTAF    G +G DSC  CL              IV QCS GGLD  VNV   L  N
Sbjct:   33 STYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKYGWTAFCGPAGPRGQDSCGRCLRVTNTGTGTQTTVRIVDQCSNGGLDLDVNVFNQLDTN 127

[Close]

BLAST of GR391162 vs. SwissProt
Match: WIN1_SOLTU (Wound-induced protein WIN1 OS=Solanum tuberosum GN=WIN1 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.185e-10
Identity = 37/88 (42.05%), Postives = 44/88 (50.00%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y +  PQN+ WD    S Y +T+D N PL+      WTAF   VG +G DSC  CL              IV QCS GGLD  VNV
Sbjct:   85 ATYHIYNPQNVGWDLNAVSAYCSTWDANKPLSWRKKYGWTAFCGPVGPRGRDSCGKCLRVTNTRTGAQTTVRIVDQCSNGGLDLDVNV 172

[Close]

BLAST of GR391162 vs. SwissProt
Match: WIN2_SOLTU (Wound-induced protein WIN2 OS=Solanum tuberosum GN=WIN2 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.639e-10
Identity = 36/88 (40.91%), Postives = 43/88 (48.86%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y +  PQN+ WD    S Y +T+D N P A      WTAF   VG +G DSC  CL              IV QCS GGLD  +NV
Sbjct:   84 ATYHIYNPQNVGWDLNAVSAYCSTWDANKPYAWRSKYGWTAFCGPVGPRGRDSCGKCLRVTNTRTGAQTTVRIVDQCSNGGLDLDINV 171

[Close]

BLAST of GR391162 vs. SwissProt
Match: PR4_PRUPE (Pathogenesis-related protein PR-4 (Fragment) OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.502e-10
Identity = 38/89 (42.70%), Postives = 44/89 (49.44%), Query Frame = 1

Query:   85 QNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHANVQ 351
            QNI+WD    SV+ AT+D + PL+      WTAF   VG  G DSC  CL              IV QCS GGLD  VNV   +  N Q
Sbjct:    1 QNINWDLRTASVFCATWDADKPLSWRSKYGWTAFCGPVGPTGQDSCGKCLLVTNTGTGAKVTVRIVDQCSNGGLDLDVNVFNQIDTNGQ 89

[Close]

BLAST of GR391162 vs. SwissProt
Match: PRP2_SOLLC (Pathogenesis-related protein P2 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.003e-9
Identity = 39/95 (41.05%), Postives = 43/95 (45.26%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            + Y L  PQNI+WD    SVY AT+D + PL       WTAF    G  G  SC  CL              IV QC  GGLD  VNV   L  N
Sbjct:   31 ATYHLYNPQNINWDLRTASVYCATWDADKPLEWRRRYGWTAFCGPAGPTGQASCGRCLRVTNTGTGTQETVRIVDQCRNGGLDLDVNVFNRLDTN 125

[Close]

BLAST of GR391162 vs. SwissProt
Match: HEVE_HEVBR (Pro-hevein OS=Hevea brasiliensis GN=HEV1 PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 1.601e-7
Identity = 34/88 (38.64%), Postives = 39/88 (44.32%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y L   Q+  WD    S Y +T+D N P +      WTAF   VG  G  SC  CL              IV QCS GGLD  VNV
Sbjct:   75 ATYHLYNSQDHGWDLNAASAYCSTWDANKPYSWRSKYGWTAFCGPVGAHGQSSCGKCLSVTNTGTGAKTTVRIVDQCSNGGLDLDVNV 162

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q41231_TOBAC (Pathogen-and wound-inducible antifungal protein CBP20 OS=Nicotiana tabacum GN=CBP20 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.384e-11
Identity = 40/88 (45.45%), Postives = 46/88 (52.27%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y +  PQN+ WD Y  S Y +T+DGN PLA      WTAF   VG +G DSC  CL              IV QCS GGLD  VNV
Sbjct:   85 ATYHIYNPQNVGWDLYAVSAYCSTWDGNKPLAWRRKYGWTAFCGPVGPRGRDSCGKCLRVTNTGTGAQTTVRIVDQCSNGGLDLDVNV 172

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q41230_TOBAC (CBP20 (Fragment) OS=Nicotiana tabacum GN=CBP20 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.384e-11
Identity = 40/88 (45.45%), Postives = 46/88 (52.27%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y +  PQN+ WD Y  S Y +T+DGN PLA      WTAF   VG +G DSC  CL              IV QCS GGLD  VNV
Sbjct:   82 ATYHIYNPQNVGWDLYAVSAYCSTWDGNKPLAWRRKYGWTAFCGPVGPRGRDSCGKCLRVTNTGTGAQTTVRIVDQCSNGGLDLDVNV 169

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q6LBM4_TOBAC (Pathogenesis-related protein 4B (Fragment) OS=Nicotiana tabacum GN=PR-4B PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.999e-10
Identity = 41/95 (43.16%), Postives = 46/95 (48.42%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            S Y L  PQNI+WD    S + AT+D + PLA      WTAF    G +G DSC  CL              IV QCS GGLD  VNV   L  N
Sbjct:   25 STYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKYGWTAFCGPAGPRGQDSCGRCLRVTNTGTGTQATVRIVDQCSNGGLDLDVNVFNQLDTN 119

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q9M7D8_PEA (Pre-hevein-like protein OS=Pisum sativum GN=PHLP PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.410e-10
Identity = 41/97 (42.27%), Postives = 48/97 (49.48%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHANVQ 351
            +NY+   PQNI+WD    SVY AT+D N PL+      WTAF   VG  G +SC  CL              IV QC  GGLD  VNV   +  N Q
Sbjct:   20 NNYN---PQNINWDYNRASVYCATWDANQPLSWRSKYGWTAFCGPVGPTGRESCGKCLRVTNIATGAQTTVRIVDQCHNGGLDLDVNVFNQIDTNKQ 113

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q9M7D9_PEA (Pathogenesis-related protein 4A OS=Pisum sativum GN=PRP4A PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.453e-10
Identity = 45/108 (41.67%), Postives = 52/108 (48.15%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN---VQLGSLASPY 375
            +NY+   PQNI+WD    SVY AT+D N PL+  +   WTAF    G  G DSC  CL              IV QCS GGLD  VNV   +  N    Q G L   Y
Sbjct:   37 NNYN---PQNINWDYNRASVYCATWDANQPLSWRH-YGWTAFCGPAGPTGRDSCGKCLRVTNTATGAQVTVRIVDQCSNGGLDLDVNVFNQIDTNGGGYQAGHLTVNY 140

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q40558_TOBAC (Pathogenesis-related protein 4A OS=Nicotiana tabacum GN=PR-4A PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.210e-9
Identity = 40/95 (42.11%), Postives = 45/95 (47.37%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            S Y L  PQNI+WD    S + AT+D + PLA      WTAF    G +G  SC  CL              IV QCS GGLD  VNV   L  N
Sbjct:   33 STYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKYGWTAFCGPAGPRGQVSCGRCLRVTNTGTGTQTTVRIVDQCSNGGLDLDVNVFNQLDTN 127

[Close]

BLAST of GR391162 vs. TrEMBL
Match: C6T1L3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.210e-9
Identity = 39/95 (41.05%), Postives = 46/95 (48.42%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHAN 345
            + Y L  P+ I W+    S Y AT+D N PLA     AWTAF   VG +G +SC  CL              IV QC+ GGLD  VNV   L  N
Sbjct:   36 ATYHLYNPEQIGWNLVTASAYCATWDANKPLAWRKKYAWTAFCGPVGPRGRESCGRCLRVTNTGTGTEATVRIVDQCANGGLDLDVNVFRQLDTN 130

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q75QH1_CAPCH (Pathogenesis-related protein 4b (Fragment) OS=Capsicum chinense GN=PR4b-Cc PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.886e-9
Identity = 40/96 (41.67%), Postives = 46/96 (47.92%), Query Frame = 1

Query:   43 EGDLPN--SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            EG   N  + Y L  PQN+ WD    S Y +T+D N PLA      WTAF   VG +G  SC  CL              IV QCS GGLD  +NV
Sbjct:   68 EGSAQNVRATYHLYNPQNVGWDLNAVSAYCSTWDANKPLAWRSKYGWTAFCGPVGPRGQASCGKCLRVTNRRTRAQTTVRIVDQCSNGGLDLDINV 163

[Close]

BLAST of GR391162 vs. TrEMBL
Match: D7L2X7_ARALY (PR4-type protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_340627 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 6.430e-9
Identity = 41/108 (37.96%), Postives = 48/108 (44.44%), Query Frame = 1

Query:   34 ECAEGDLPN--SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNVLYLLHANVQ 351
            + A    PN  + Y +  P   +WD Y  S Y +T+DGN PL       WTAF   VG +G DSC  CL              IV QCS GGLD    V   L  N Q
Sbjct:   18 KAAAQSAPNVRATYHIYNPAQNNWDLYRVSAYCSTWDGNQPLEWRQRYGWTAFCGPVGPRGRDSCGRCLRVTNTATGTQATVRIVDQCSNGGLDLDEGVFRQLDTNGQ 125

[Close]

BLAST of GR391162 vs. TrEMBL
Match: Q8RW37_SOLLC (Pi1 protein (Fragment) OS=Solanum lycopersicum GN=pi1 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 8.398e-9
Identity = 35/88 (39.77%), Postives = 43/88 (48.86%), Query Frame = 1

Query:   61 SNYDLDCPQNIDWDNYMDSVYRATFDGNHPLACVY*AAWTAFRASVGTQG*DSCAFCLXXXXXXXXXXXXWLIVAQCSTGGLDFGVNV 324
            + Y +  PQN+ WD    S Y +T+D N P +      WTAF   VG +G DSC  CL              IV QCS GGLD  +NV
Sbjct:   10 ATYHIYNPQNVGWDLNAVSAYCSTWDANKPYSWRSKYGWTAFCGPVGPRGRDSCGKCLRVTNTRTGAQTTVRIVDQCSNGGLDLDINV 97

The following BLAST results are available for this feature:

BLAST of GR391162 vs. SwissProt
BLAST of GR391162 vs. TrEMBL

BLAST of GR391162 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
PR4B_TOBAC	1.399e-11	43.16	Pathogenesis-related protein PR-4B OS=Nicotiana ta...	[more]
PR4A_TOBAC	1.399e-11	43.16	Pathogenesis-related protein PR-4A OS=Nicotiana ta...	[more]
WIN1_SOLTU	1.185e-10	42.05	Wound-induced protein WIN1 OS=Solanum tuberosum GN...	[more]
WIN2_SOLTU	2.639e-10	40.91	Wound-induced protein WIN2 OS=Solanum tuberosum GN...	[more]
PR4_PRUPE	4.502e-10	42.70	Pathogenesis-related protein PR-4 (Fragment) OS=Pr...	[more]
PRP2_SOLLC	1.003e-9	41.05	Pathogenesis-related protein P2 OS=Solanum lycoper...	[more]
HEVE_HEVBR	1.601e-7	38.64	Pro-hevein OS=Hevea brasiliensis GN=HEV1 PE=1 SV=2	[more]

BLAST of GR391162 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q41231_TOBAC	1.384e-11	45.45	Pathogen-and wound-inducible antifungal protein CB...	[more]
Q41230_TOBAC	1.384e-11	45.45	CBP20 (Fragment) OS=Nicotiana tabacum GN=CBP20 PE=...	[more]
Q6LBM4_TOBAC	1.999e-10	43.16	Pathogenesis-related protein 4B (Fragment) OS=Nico...	[more]
Q9M7D8_PEA	3.410e-10	42.27	Pre-hevein-like protein OS=Pisum sativum GN=PHLP P...	[more]
Q9M7D9_PEA	4.453e-10	41.67	Pathogenesis-related protein 4A OS=Pisum sativum G...	[more]
Q40558_TOBAC	2.210e-9	42.11	Pathogenesis-related protein 4A OS=Nicotiana tabac...	[more]
C6T1L3_SOYBN	2.210e-9	41.05	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q75QH1_CAPCH	2.886e-9	41.67	Pathogenesis-related protein 4b (Fragment) OS=Caps...	[more]
D7L2X7_ARALY	6.430e-9	37.96	PR4-type protein OS=Arabidopsis lyrata subsp. lyra...	[more]
Q8RW37_SOLLC	8.398e-9	39.77	Pi1 protein (Fragment) OS=Solanum lycopersicum GN=...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000726	Glycoside hydrolase, family 19, catalytic	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 1..37 score: 1.8
IPR001153	Barwin	PANTHER	PTHR22595:SF1	WOUND-INDUCED PROTEIN WIN-RELATED	coord: 1..37 score: 1.8
IPR001153	Barwin	PFAM	PF00967	Barwin	coord: 1..44 score: 5.2
IPR001153	Barwin	PROFILE	PS51174	BARWIN_3	coord: 1..55 score: 12
IPR014733	Barwin-like endoglucanase	GENE3D	2.40.40.10	no description	coord: 1..42 score: 3.3
None	No IPR available	SEG	seg	seg	coord: 8..19 score
None	No IPR available	SEG	seg	seg	coord: 34..46 score: NAcoord: 51..82 score
None	No IPR available	SEG	seg	seg	coord: 15..33 score: NAcoord: 37..55 score
None	No IPR available	SEG	seg	seg	coord: 4..20 score
None	No IPR available	SEG	seg	seg	coord: 6..22 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR391162 ID=GR391162; Name=GR391162; organism=Cicer arietinum; type=EST; length=628bp
GACGTTGTTGTATCTAAAGCCACCGATGTTATTGAGTGTGCTGAGGGTGA
TCTTCCTAATAGCAACTATGATTTGGACTGCCCTCAGAATATTGATTGGG
ACAATTACATGGATAGTGTTTACCGTGCTACATTTGATGGGAACCATCCC
TTGGCTTGTGTATATTGAGCTGCTTGGACTGCCTTTCGTGCCTCTGTTGG
GACACAAGGATAAGATTCTTGTGCCTTTTGCTTGACTGTGACGACTACCG
CCACAGATTCTCGTGTCACATGGTTAATAGTTGCTCAATGCTCCACTGGC
GGTTTGGACTTCGGAGTTAATGTCTTATATCTGCTTCATGCTAATGTTCA
ACTTGGATCGTTAGCGTCTCCTTACTGTTGACTCTACCTGTTGCTGATTT
GCCGACATGAATTTCCTTATTGCTACCTATCCCTTTGTTTATATGCCCCG
CACTCTTTTCTGATCACACTTTTATTATGTTCTAGCTCTCGTTTCATCTC
TTCATATATTTATATTATTACCGGTCCTGTCTCTTTCTTTTTCCCCCTCT
TCAATTTTTCCCCATTCGCGGTTTCTTTTGTCCTTTTCTCCCCTTTTTTT
CCCCTCCCTCCCCCTCTTTTCTCTCTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004568	chitinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006032	chitin catabolic process
GO:0016998	cell wall macromolecule catabolic process
GO:0042742	defense response to bacterium
GO:0050832	defense response to fungus

Vocabulary: **INTERPRO**
Term	Definition
IPR000726	Glyco_hydro_19_cat
IPR001153	Barwin_dom
IPR014733	Barwin-like endoglucanase

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