GR391171

EST Overview


Name	GR391171
Unique Name	GR391171
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	657

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIT_CAEEL (Probable endochitinase OS=Caenorhabditis elegans GN=cht-1 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.787e-11
Identity = 43/121 (35.54%), Postives = 65/121 (53.72%), Query Frame = 1

Query:   16 FNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIAN---NLYWVNVMTYDL---------FSSDGYPTTIQFPCSFEKPYWSIHCTRRY 342
            F+G+D+DWEYPS  TDM N+ +L+KE +AA  + A  +GK  L+++AAV       P     G DI N   N  ++ +M+YD          F+S  Y TT + P  +    W++  + RY
Sbjct:  171 FDGIDIDWEYPSGATDMANYVALVKELKAACESEAGSTGKDRLLVTAAVAAG----PATIDAGYDIPNLAPNFDFILLMSYDFFGAWASLVGFNSPLYATT-ELPAEWNG--WNVDSSARY 284

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIA_MOUSE (Acidic mammalian chitinase OS=Mus musculus GN=Chia PE=1 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 2.688e-8
Identity = 41/96 (42.71%), Postives = 53/96 (55.21%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAV-GGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS-DGY 273
            R   F+GLDLDWEYP S      D   F  L+KE R A    A  S +P LM++AAV GG   I      P  +++  L +++VMTYDL  S +GY
Sbjct:  128 RQYGFDGLDLDWEYPGSRGSPPQDKHLFTVLVKEMREAFEQEAIESNRPRLMVTAAVAGGISNIQAGYEIP--ELSKYLDFIHVMTYDLHGSWEGY 221

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIT1_HUMAN (Chitotriosidase-1 OS=Homo sapiens GN=CHIT1 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.511e-8
Identity = 37/91 (40.66%), Postives = 46/91 (50.55%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS 264
            R  +F+GLDLDWEYP S      D   F +L+++   A    A  SGK  L+LSAAV           Y    IA NL +VN+M YD   S
Sbjct:  128 RKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVD-AGYEVDKIAQNLDFVNLMAYDFHGS 217

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIA_RAT (Acidic mammalian chitinase OS=Rattus norvegicus GN=Chia PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 4.585e-8
Identity = 41/96 (42.71%), Postives = 52/96 (54.17%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAV-GGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS-DGY 273
            R   F+GLDLDWEYP S      D   F  L+KE R A    A  S +P LM++AAV  G   I      P  +++  L +++VMTYDL  S DGY
Sbjct:  128 RQYGFDGLDLDWEYPGSRGSPPQDKHLFTVLVKELREAFEQEAIESNRPRLMVTAAVAAGISNIQAGYEIP--ELSQYLDFIHVMTYDLHGSWDGY 221

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIT1_MOUSE (Chitotriosidase-1 OS=Mus musculus GN=Chit1 PE=1 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 1.334e-7
Identity = 37/91 (40.66%), Postives = 49/91 (53.85%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYP----SSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS 264
            R   F+GLDLDWE+P    S   D   F +LI++   A    A  SGK  L+L+AAV  SD+      Y    IA +L ++N+M YD  SS
Sbjct:  128 RTQGFDGLDLDWEFPGGRGSPTVDKERFTALIQDLAKAFQEEAQSSGKERLLLTAAV-PSDRGLVDAGYEVDKIAQSLDFINLMAYDFHSS 217

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIA_HUMAN (Acidic mammalian chitinase OS=Homo sapiens GN=CHIA PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 5.063e-7
Identity = 38/96 (39.58%), Postives = 49/96 (51.04%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAV-GGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS-DGY 273
            R   F+GLD DWEYP S      D   F  L++E R A    A    KP LM++AAV  G   I      P   ++  L +++VMTYDL  S +GY
Sbjct:  128 RQYEFDGLDFDWEYPGSRGSPPQDKHLFTVLVQEMREAFEQEAKQINKPRLMVTAAVAAGISNIQSGYEIP--QLSQYLDYIHVMTYDLHGSWEGY 221

HSP 2 Score: 21.557 bits (44), Expect = 5.063e-7
Identity = 11/28 (39.29%), Postives = 16/28 (57.14%), Query Frame = 2

Query:  362 GALKSKLPLALPFYVNHRILSEPTIHGL 445
            GA   KL +  P Y ++ ILS P+  G+
Sbjct:  254 GAPAEKLIVGFPTYGHNFILSNPSNTGI 281

[Close]

BLAST of GR391171 vs. SwissProt
Match: CH3L4_MOUSE (Chitinase-3-like protein 4 OS=Mus musculus GN=Chi3l4 PE=1 SV=2)

HSP 1 Score: 49.2914 bits (116), Expect = 5.099e-7
Identity = 31/95 (32.63%), Postives = 51/95 (53.68%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFS-SDGY 273
            R  NF+GL+LDW+YP S      D   F  L++E R A    ++++  P L+L++   G   +     Y   +++ +L ++ VMTYDL    +GY
Sbjct:  128 RQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVQEMRKAFEEESTLNHIPRLLLTSTGAGFIDVIK-SGYKIPELSQSLDYIQVMTYDLHDPKNGY 221

HSP 2 Score: 25.0238 bits (53), Expect = 5.099e-7
Identity = 19/56 (33.93%), Postives = 26/56 (46.43%), Query Frame = 2

Query:  278 PRYSSPAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGL 445
            P Y SP  +     L  V   IT     GA   KL +  P Y +  ILS+P+ +G+
Sbjct:  227 PLYKSPYDIGKSADLN-VDSIITYWKDHGAASEKLIVGFPAYGHTFILSDPSKNGI 281

[Close]

BLAST of GR391171 vs. SwissProt
Match: CHIT2_DROME (Probable chitinase 2 OS=Drosophila melanogaster GN=Cht2 PE=1 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 6.554e-7
Identity = 33/91 (36.26%), Postives = 46/91 (50.55%), Query Frame = 1

Query:    4 RDNNFNGLDLDWEYPSSN----TDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS 264
            R  NF+GLDLDWEYP+       D  NF  L KE R     H        L+L++A+G S ++    Y   Q I+  L ++++M YD   S
Sbjct:  156 RKYNFDGLDLDWEYPTQRKGKPADRENFVLLTKELREEFDEHG-------LLLTSAIGASKKVIDEAYDVRQ-ISRYLDYLHIMCYDYHGS 238

HSP 2 Score: 23.8682 bits (50), Expect = 6.554e-7
Identity = 16/42 (38.10%), Postives = 21/42 (50.00%), Query Frame = 2

Query:  284 YSSP--APLKNPTGLFIVHEGITQCIALGALKSKLPLALPFY 403
            Y++P  AP  +P     V   I   + LGA   KL + LPFY
Sbjct:  245 YNAPLTAPADDPLS---VKFSIDYLLKLGAPPEKLVMGLPFY 283

[Close]

BLAST of GR391171 vs. TrEMBL
Match: B7FNG8_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 8.116e-45
Identity = 73/104 (70.19%), Postives = 83/104 (79.81%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR+NNF+GLDLDWEYPSS+T+  NFGSLI+E RAAV   +  SGKP L+LSAAVGGSDQITPL YYPGQD+ANNL WVNVMTYDL+ SD  PT  Q P   + P
Sbjct:  131 ARNNNFHGLDLDWEYPSSDTEKANFGSLIREWRAAVTTESRSSGKPALLLSAAVGGSDQITPLKYYPGQDVANNLDWVNVMTYDLYISDTNPTQTQPPAPLKNP 234

HSP 2 Score: 52.7582 bits (125), Expect = 8.116e-45
Identity = 27/55 (49.09%), Postives = 34/55 (61.82%), Query Frame = 2

Query:  293 PAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGLSLKA 457
            PAPLKNPTGLF   EGI + + LG  K+KL L LP+Y     L++P    +  KA
Sbjct:  228 PAPLKNPTGLFSADEGINKWVGLGVPKNKLALGLPYYGYKWRLADPNNRRIYSKA 282

[Close]

BLAST of GR391171 vs. TrEMBL
Match: A1YZD2_ROBPS (Chitinase-related agglutinin (Fragment) OS=Robinia pseudoacacia PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 1.950e-43
Identity = 74/104 (71.15%), Postives = 82/104 (78.85%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR NNF+GLDLDWEYPSS+ D TNF SLI+E RAAVA  +S SG P L+LSAAVGGSDQITPL YYPG+ IANNL WVNVMTYDL++SD YPT  Q P     P
Sbjct:  102 ARRNNFHGLDLDWEYPSSDIDKTNFASLIREWRAAVATESSTSGTPALLLSAAVGGSDQITPLKYYPGEAIANNLDWVNVMTYDLYTSDSYPTLTQPPAPLFYP 205

HSP 2 Score: 49.6766 bits (117), Expect = 1.950e-43
Identity = 27/51 (52.94%), Postives = 31/51 (60.78%), Query Frame = 2

Query:  293 PAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGL 445
            PAPL  P G+F   EGIT+ I  G  +SKL L LPFY     LS+P  HGL
Sbjct:  199 PAPLFYPRGIFSADEGITKWIQSGVPESKLALGLPFYGFKWRLSDPNNHGL 249

[Close]

BLAST of GR391171 vs. TrEMBL
Match: B9VQ35_PYRPY (Class V chitinase OS=Pyrus pyrifolia PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.897e-21
Identity = 50/101 (49.50%), Postives = 66/101 (65.35%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAV-GGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCS 300
            AR  NF+GLDLDWEYPSS T+MTNFG+L+ E R AVA  +  +GK  L+L+AAV   +D  T  + YP Q I+N+L W+NVM YD +    +P     P +
Sbjct:  128 ARSYNFHGLDLDWEYPSSTTEMTNFGTLLTEWRTAVANESKTTGKTALLLAAAVFRSADYYT--INYPFQSISNSLDWINVMAYDFYGPGWFPNNTAPPAA 226

HSP 2 Score: 27.7202 bits (60), Expect = 5.897e-21
Identity = 16/41 (39.02%), Postives = 19/41 (46.34%), Query Frame = 2

Query:  338 GITQCIALGALKSKLPLALPFYVNHRILSEPTIHGLSLKAN 460
            GIT  I  G    K+ L LPFY     L +   HG+   AN
Sbjct:  237 GITSWIQAGLAPKKIVLGLPFYGYAWRLLDANNHGIFAPAN 277

[Close]

BLAST of GR391171 vs. TrEMBL
Match: D7T548_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_149.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001068001 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.343e-19
Identity = 50/104 (48.08%), Postives = 65/104 (62.50%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR N F+GLDLDWEYP++ TDMTNFG+L+ E RAAVA  +  SGK  L+L+AAV  + +I   L YP   I+ +L W+N M YD F   G+    + P     P
Sbjct:  128 ARSNGFSGLDLDWEYPTTTTDMTNFGTLLTEWRAAVANESKNSGKAALLLTAAVYYASKIEE-LSYPVSSISASLDWINAMAYD-FYGPGWSQVTRLPAQLYDP 229

HSP 2 Score: 26.1794 bits (56), Expect = 2.343e-19
Identity = 17/51 (33.33%), Postives = 21/51 (41.18%), Query Frame = 2

Query:  293 PAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGL 445
            PA L +P   +  + GI   I  G    K+ L  PFY     L     HGL
Sbjct:  223 PAQLYDPGSQYNGNYGIVDWIQAGLSAKKIVLGFPFYGYAWRLVNANNHGL 273

[Close]

BLAST of GR391171 vs. TrEMBL
Match: D7MG84_ARALY (Glycosyl hydrolase family 18 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_329582 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.614e-19
Identity = 50/86 (58.14%), Postives = 65/86 (75.58%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLF 258
            AR   F+GLDLDWEYPSS T+MTNFG+L++E R+AV A AS SGKP L+L+AAV  S+    +L YP Q +A++L WVN+M YD +
Sbjct:  127 ARSYGFHGLDLDWEYPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVL-YPVQSVASSLDWVNLMAYDFY 211

[Close]

BLAST of GR391171 vs. TrEMBL
Match: D7T550_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_149.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001074001 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.720e-19
Identity = 50/104 (48.08%), Postives = 64/104 (61.54%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR N F+GLDLDWEYP + TDMTNFG+L+ E RAAVA  +  SGK  L+L+AAV  + +I   L YP   I+ +L W+N M YD F   G+    + P     P
Sbjct:   93 ARSNGFSGLDLDWEYPITTTDMTNFGTLLTEWRAAVANESKNSGKAALLLTAAVYYASKI-ERLSYPVSSISASLDWINAMAYD-FYGPGWSQVTRLPAQLYDP 194

HSP 2 Score: 26.1794 bits (56), Expect = 6.720e-19
Identity = 17/51 (33.33%), Postives = 21/51 (41.18%), Query Frame = 2

Query:  293 PAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGL 445
            PA L +P   +  + GI   I  G    K+ L  PFY     L     HGL
Sbjct:  188 PAQLYDPGSQYNGNYGIVDWIQAGLSAKKIVLGFPFYGYAWRLVNANNHGL 238

[Close]

BLAST of GR391171 vs. TrEMBL
Match: D7SSN5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_206.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00003718001 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 8.718e-19
Identity = 49/104 (47.12%), Postives = 65/104 (62.50%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR N F+GLDLDWEYP++ TDM+NFG+L+ E RAAVA  +  SGK  L+L+AAV  + +I   L YP   I+ +L W+N M YD F   G+    + P     P
Sbjct:  128 ARSNGFSGLDLDWEYPTTTTDMSNFGTLLTEWRAAVANESKNSGKAALLLTAAVYYASKIEG-LSYPVSSISASLDWINAMAYD-FYGPGWSQVTRLPAQLYDP 229

HSP 2 Score: 26.1794 bits (56), Expect = 8.718e-19
Identity = 17/51 (33.33%), Postives = 21/51 (41.18%), Query Frame = 2

Query:  293 PAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGL 445
            PA L +P   +  + GI   I  G    K+ L  PFY     L     HGL
Sbjct:  223 PAQLYDPGSQYNGNYGIVDWIQAGLSAKKIVLGFPFYGYAWRLVNANNHGL 273

[Close]

BLAST of GR391171 vs. TrEMBL
Match: Q43591_TOBAC (Chitinase/lysozyme OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.133e-18
Identity = 44/93 (47.31%), Postives = 59/93 (63.44%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPT 279
            AR   F+GLDLDWEYP S TDMTN G L+ E R A+   A  SG+  L+L+AAV  S ++   L YP + +A NL W+N+M YD +  +  P+
Sbjct:  128 ARQFGFHGLDLDWEYPLSATDMTNLGILLNEWRTAINMEARNSGRAALLLTAAVSYSPRVNG-LNYPVESVARNLNWINLMAYDFYGPNWSPS 219

HSP 2 Score: 29.261 bits (64), Expect = 1.133e-18
Identity = 20/57 (35.09%), Postives = 24/57 (42.11%), Query Frame = 2

Query:  287 SSPAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGLSLKA 457
            +S A L +P       +GI   I  G    KL L +PFY     L  P IH L   A
Sbjct:  222 NSHAQLFDPVNHISGSDGINAWIQAGVPTKKLVLGIPFYGYAWRLVNPNIHDLRAPA 278

[Close]

BLAST of GR391171 vs. TrEMBL
Match: Q7DM11_TOBAC (Chitinase, class V (Fragment) OS=Nicotiana tabacum GN=chi-V PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.134e-18
Identity = 44/93 (47.31%), Postives = 59/93 (63.44%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPT 279
            AR   F+GLDLDWEYP S TDMTN G L+ E R A+   A  SG+  L+L+AAV  S ++   L YP + +A NL W+N+M YD +  +  P+
Sbjct:  119 ARQFGFHGLDLDWEYPLSATDMTNLGILLNEWRTAINMEARNSGRAALLLTAAVSYSPRVNG-LNYPVESVARNLNWINLMAYDFYGPNWSPS 210

HSP 2 Score: 29.261 bits (64), Expect = 1.134e-18
Identity = 20/57 (35.09%), Postives = 24/57 (42.11%), Query Frame = 2

Query:  287 SSPAPLKNPTGLFIVHEGITQCIALGALKSKLPLALPFYVNHRILSEPTIHGLSLKA 457
            +S A L +P       +GI   I  G    KL L +PFY     L  P IH L   A
Sbjct:  213 NSHAQLFDPVNHISGSDGINAWIQAGVPTKKLVLGIPFYGYAWRLVNPNIHDLRAPA 269

[Close]

BLAST of GR391171 vs. TrEMBL
Match: Q0WT03_ARATH (Putative chitinase OS=Arabidopsis thaliana GN=At4g19810 PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.694e-18
Identity = 49/86 (56.98%), Postives = 64/86 (74.42%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLF 258
            AR   F+GLDLDWEYPSS T+MTNFG+L++E R+AV A AS SGKP L+L+AAV  S+    +L YP   +A++L WVN+M YD +
Sbjct:   85 ARSYGFHGLDLDWEYPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVL-YPVSAVASSLDWVNLMAYDFY 169

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19810.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10764151-10765753 REVERSE LENGTH=379)

HSP 1 Score: 96.6709 bits (239), Expect = 1.201e-20
Identity = 49/86 (56.98%), Postives = 64/86 (74.42%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLF 258
            AR   F+GLDLDWEYPSS T+MTNFG+L++E R+AV A AS SGKP L+L+AAV  S+    +L YP   +A++L WVN+M YD +
Sbjct:  127 ARSYGFHGLDLDWEYPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVL-YPVSAVASSLDWVNLMAYDFY 211

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19820.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10767436-10768614 REVERSE LENGTH=366)

HSP 1 Score: 82.0333 bits (201), Expect = 3.061e-16
Identity = 43/88 (48.86%), Postives = 59/88 (67.05%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSS 264
            AR   F+GLDL+W+YPS  T+M NFG L++E R AV A A  SGKP L+L+AAV  S     +L +P   +A++L WVN++ YD + S
Sbjct:  125 ARSCGFHGLDLNWKYPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVFYSYSYYSVL-HPVNAVADSLDWVNLVAYDFYES 211

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19730.1 (| Symbols: | Glycosyl hydrolase superfamily protein | chr4:10733864-10734975 REVERSE LENGTH=332)

HSP 1 Score: 82.0333 bits (201), Expect = 3.061e-16
Identity = 42/86 (48.84%), Postives = 57/86 (66.28%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLF 258
            AR N F+GLDL WEYP S+ +MT+FG+L+ E RAAV A +  S KP L+L+AAV  S  +     YP Q +  +L WVN++ YD +
Sbjct:  118 ARSNGFHGLDLAWEYPYSDHEMTDFGNLVGELRAAVEAESRRSSKPTLLLTAAVYYS-SVYKTFTYPVQVMRESLDWVNIIAYDFY 202

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19800.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10760830-10762104 REVERSE LENGTH=398)

HSP 1 Score: 78.9518 bits (193), Expect = 2.592e-15
Identity = 45/104 (43.27%), Postives = 62/104 (59.62%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR   F+GLDLDWEYP +  +M +FG L++E R+AV A ++ SG   L+L+AAV  S      + YP   I+N+L W+N+M YD F   G+ T    P S   P
Sbjct:  106 ARSYGFHGLDLDWEYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNYQG-VPYPVLAISNSLDWINLMAYD-FYGPGWSTVTGPPASLYLP 207

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19760.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10750381-10752028 FORWARD LENGTH=369)

HSP 1 Score: 78.9518 bits (193), Expect = 2.592e-15
Identity = 43/94 (45.74%), Postives = 60/94 (63.83%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTT 282
            AR  +F GLDL WEYPS++ +MTNFG L++E RAAV   +  + +  L+L+AAV  S Q    + YP + IA+NL +VN+M YD +     P T
Sbjct:  123 ARKKDFYGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYDG-VEYPVKAIADNLDFVNIMAYDFYGPGWSPVT 215

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19750.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10745682-10747127 REVERSE LENGTH=362)

HSP 1 Score: 78.1814 bits (191), Expect = 4.421e-15
Identity = 44/97 (45.36%), Postives = 59/97 (60.82%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLY---YPGQDIANNLYWVNVMTYDLFSSDGYPTT 282
            AR  +F GLDL WEYPS++ +M NFG L+KE RAAV   +  + +  L+L+AAV      +P  Y   YP Q IA+NL +VN+M YD +     P T
Sbjct:  115 ARKKDFYGLDLAWEYPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAV----YYSPDYYGEEYPVQAIADNLDFVNIMAYDFYGPGWSPVT 207

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19770.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10753310-10754181 REVERSE LENGTH=261)

HSP 1 Score: 70.8626 bits (172), Expect = 4.399e-13
Identity = 38/86 (44.19%), Postives = 52/86 (60.47%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLF 258
            AR   F+GLDLDWEYP +  +M++F  L+KE R AV   A  S  PVL+L+A V  S     ++ YP + I+  L WVN+  YD +
Sbjct:   19 ARSYGFDGLDLDWEYPRNAAEMSDFAELLKEWRYAVQGEAYSSELPVLILTATVYYSSNYNGVV-YPVKFISELLDWVNIKAYDFY 103

HSP 2 Score: 20.7866 bits (42), Expect = 4.399e-13
Identity = 11/35 (31.43%), Postives = 15/35 (42.86%), Query Frame = 2

Query:  338 GITQCIALGALKSKLPLALPFYVNHRILSEPTIHG 442
            G+   I  G    K  L  P+Y     L++P  HG
Sbjct:  129 GVKDWIDAGLPAEKAVLGFPYYGWAWTLADPKNHG 163

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19740.1 (| Symbols: | Glycosyl hydrolase superfamily protein | chr4:10739567-10740302 REVERSE LENGTH=211)

HSP 1 Score: 68.5514 bits (166), Expect = 3.503e-12
Identity = 40/104 (38.46%), Postives = 58/104 (55.77%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR   F GLDL WEYP+++ +M NFG L++E R+AV   +  +G   L+L+AAV  +      + YP Q I  +L WVN++ Y+ +   G  T I  P     P
Sbjct:   19 ARSLGFYGLDLAWEYPNNDVEMNNFGKLLQEWRSAVEVESQRTGIRPLLLTAAVYYTSDYNS-VSYPVQAINRSLDWVNLIAYEFY---GLTTEIGPPAGLYDP 118

[Close]

BLAST of GR391171 vs. TAIR peptide
Match: AT4G19720.1 (| Symbols: | Glycosyl hydrolase family protein with chitinase insertion domain | chr4:10730363-10731695 REVERSE LENGTH=363)

HSP 1 Score: 68.1662 bits (165), Expect = 4.575e-12
Identity = 40/104 (38.46%), Postives = 60/104 (57.69%), Query Frame = 1

Query:    1 ARDNNFNGLDLDWEYPSSNTDMTNFGSLIKECRAAVAAHASISGKPVLMLSAAVGGSDQITPLLYYPGQDIANNLYWVNVMTYDLFSSDGYPTTIQFPCSFEKP 312
            AR   F+GLDL W+YP  + +M NFG L+++ R A+   A  + +  L+L+AAV  S  +   + YP ++I   L WVN++ YD +SS    TTI  P +   P
Sbjct:  116 ARYYRFDGLDLVWKYPKDDVEMRNFGQLLEQWREAIEDDAERTERMPLLLTAAVYYS-PVYDSVSYPIREIKKKLDWVNLIAYDFYSSS---TTIGPPAALFDP 215

The following BLAST results are available for this feature:

BLAST of GR391171 vs. SwissProt
BLAST of GR391171 vs. TrEMBL
BLAST of GR391171 vs. TAIR peptide

BLAST of GR391171 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
CHIT_CAEEL	5.787e-11	35.54	Probable endochitinase OS=Caenorhabditis elegans G...	[more]
CHIA_MOUSE	2.688e-8	42.71	Acidic mammalian chitinase OS=Mus musculus GN=Chia...	[more]
CHIT1_HUMAN	3.511e-8	40.66	Chitotriosidase-1 OS=Homo sapiens GN=CHIT1 PE=1 SV...	[more]
CHIA_RAT	4.585e-8	42.71	Acidic mammalian chitinase OS=Rattus norvegicus GN...	[more]
CHIT1_MOUSE	1.334e-7	40.66	Chitotriosidase-1 OS=Mus musculus GN=Chit1 PE=1 SV...	[more]
CHIA_HUMAN	5.063e-7	39.58	Acidic mammalian chitinase OS=Homo sapiens GN=CHIA...	[more]
CH3L4_MOUSE	5.099e-7	32.63	Chitinase-3-like protein 4 OS=Mus musculus GN=Chi3...	[more]
CHIT2_DROME	6.554e-7	36.26	Probable chitinase 2 OS=Drosophila melanogaster GN...	[more]

BLAST of GR391171 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FNG8_MEDTR	8.116e-45	70.19	Putative uncharacterized protein OS=Medicago trunc...	[more]
A1YZD2_ROBPS	1.950e-43	71.15	Chitinase-related agglutinin (Fragment) OS=Robinia...	[more]
B9VQ35_PYRPY	5.897e-21	49.50	Class V chitinase OS=Pyrus pyrifolia PE=2 SV=1	[more]
D7T548_VITVI	2.343e-19	48.08	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7MG84_ARALY	2.614e-19	58.14	Glycosyl hydrolase family 18 protein OS=Arabidopsi...	[more]
D7T550_VITVI	6.720e-19	48.08	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SSN5_VITVI	8.718e-19	47.12	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q43591_TOBAC	1.133e-18	47.31	Chitinase/lysozyme OS=Nicotiana tabacum PE=2 SV=1	[more]
Q7DM11_TOBAC	1.134e-18	47.31	Chitinase, class V (Fragment) OS=Nicotiana tabacum...	[more]
Q0WT03_ARATH	1.694e-18	56.98	Putative chitinase OS=Arabidopsis thaliana GN=At4g...	[more]

BLAST of GR391171 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT4G19810.1	1.201e-20	56.98	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19820.1	3.061e-16	48.86	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19730.1	3.061e-16	48.84	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT4G19800.1	2.592e-15	43.27	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19760.1	2.592e-15	45.74	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19750.1	4.421e-15	45.36	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19770.1	4.399e-13	44.19	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]
AT4G19740.1	3.503e-12	38.46	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT4G19720.1	4.575e-12	38.46	\| Symbols: \| Glycosyl hydrolase family protein wi...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001223	Glycoside hydrolase, family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 2..96 score: 1
IPR001579	Glycoside hydrolase, chitinase active site	PROSITE	PS01095	CHITINASE_18	coord: 6..14 score
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 2..111 score: 3.2
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..98 score: 1.3
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 1..88 score: 6.9
None	No IPR available	PANTHER	PTHR11177:SF22	ARABIDOPSIS THALIANA CHITINASE	coord: 1..88 score: 6.9
None	No IPR available	SEG	seg	seg	coord: 16..37 score
None	No IPR available	SEG	seg	seg	coord: 38..50 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR391171 ID=GR391171; Name=GR391171; organism=Cicer arietinum; type=EST; length=657bp
GCTAGAGATAACAACTTCAATGGCCTTGATCTTGATTGGGAGTACCCGTC
GTCCAACACGGACATGACCAACTTCGGTTCACTCATCAAAGAGTGCAGAG
CTGCTGTGGCCGCACATGCTAGCATTTCTGGCAAGCCTGTACTGATGTTA
TCAGCTGCAGTGGGTGGCTCTGACCAGATAACCCCATTGCTGTACTACCC
AGGTCAAGATATTGCAAACAACTTGTACTGGGTCAATGTAATGACTTACG
ACCTTTTCTCCTCAGATGGTTATCCAACCACGATACAGTTCCCCTGCTCC
TTTGAAAAACCCTACTGGTCTATTCATTGTACACGAAGGTATCACACAAT
GTATTGCCCTAGGAGCACTAAAAAGCAAATTACCCCTGGCATTACCCTTC
TATGTCAATCACCGGATTTTGTCAGAACCTACCATCCATGGATTGTCCTT
AAAAGCTAATTACGTACTCGTTTCATTGCATTCATATTGATCTTAACTAT
CGCTCCCCCCCCACATTTGTTCCCCTTTTTTACTCTGTTACCAACTACCC
CTCTTTATCCACACCATTTCTTCCTATCTTACGACCCCTCCCACCTTTTT
TTTCCAGGATTCCTCACCCCCCCCAACTGGATTTGCTCTCTATATTTTCC
TCCCCCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001223	Glyco_hydro18_cat
IPR001579	Glyco_hydro_18_chit_AS
IPR013781	Glyco_hydro_catalytic_dom
IPR017853	Glycoside_hydrolase_SF

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