GR391359

EST Overview


Name	GR391359
Unique Name	GR391359
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	658

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR391359 vs. TrEMBL
Match: Q8LPE6_CICAR (Epoxide hydrolase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 4.853e-24
Identity = 54/65 (83.08%), Postives = 57/65 (87.69%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ GELDMV  SLNMKEYI GGGFKEDVP+  EVIVQKGVA FNNQEAAEEISNHI EFIKKF
Sbjct:  211 VKFITGELDMVYTSLNMKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAEEISNHIYEFIKKF 275

HSP 2 Score: 29.6462 bits (65), Expect = 4.853e-24
Identity = 18/34 (52.94%), Postives = 20/34 (58.82%), Query Frame = -2

Query:  400 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAK 501
            VSK E  G    GL  YR++ LN E M PW GAK
Sbjct:  174 VSKFEKTG-FTGGLNYYRNLNLNWELMAPWRGAK 206

[Close]

BLAST of GR391359 vs. TrEMBL
Match: A2Q321_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g6v2 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 5.178e-23
Identity = 52/65 (80.00%), Postives = 57/65 (87.69%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LDMV  SLNMKEYI GGGFKEDVP+  EVI+QKGVA FNNQEAAEEISNHI EFIKKF
Sbjct:  255 VKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFIKKF 319

HSP 2 Score: 27.7202 bits (60), Expect = 5.178e-23
Identity = 17/36 (47.22%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G     L  YR+  LN E M PW G K K
Sbjct:  218 VSKFEKTG-FTGALNYYRNFNLNWELMAPWNGVKIK 252

[Close]

BLAST of GR391359 vs. TrEMBL
Match: A2Q324_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g9v2 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.141e-22
Identity = 52/65 (80.00%), Postives = 57/65 (87.69%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LDMV  SLNMKEYI GGGFKEDVP+  EVI+QKGVA FNNQEAAEEISNHI EFIKKF
Sbjct:  255 VKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFIKKF 319

HSP 2 Score: 26.5646 bits (57), Expect = 1.141e-22
Identity = 16/36 (44.44%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G     L  YR+  +N E M PW G K K
Sbjct:  218 VSKFEKTG-FTGALNYYRNFNVNWELMAPWNGVKIK 252

[Close]

BLAST of GR391359 vs. TrEMBL
Match: B7FJ70_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.271e-22
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LDMV  SLNMKEYI GGGFKEDVP+  EVI+QKG A FNNQEAAEEISNHI EFIKKF
Sbjct:  255 VKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGGAHFNNQEAAEEISNHIYEFIKKF 319

HSP 2 Score: 27.7202 bits (60), Expect = 3.271e-22
Identity = 17/36 (47.22%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G     L  YR+  LN E M PW G K K
Sbjct:  218 VSKFEKTG-FTGALNYYRNFNLNWELMAPWNGVKIK 252

[Close]

BLAST of GR391359 vs. TrEMBL
Match: B7FJM9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 4.256e-22
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LDMV  SLNMKEYI GGGFKEDVP+  EVI+QKGVA FNNQEAAEEISNHI EF KKF
Sbjct:  255 VKFITGDLDMVYTSLNMKEYIHGGGFKEDVPNLEEVIIQKGVAHFNNQEAAEEISNHIYEFTKKF 319

HSP 2 Score: 26.5646 bits (57), Expect = 4.256e-22
Identity = 16/36 (44.44%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G     L  YR+  +N E M PW G K K
Sbjct:  218 VSKFEKTG-FTGALNYYRNFNVNWELMAPWNGVKIK 252

[Close]

BLAST of GR391359 vs. TrEMBL
Match: A2Q320_MEDTR (Epoxide hydrolase OS=Medicago truncatula GN=MtrDRAFT_AC154391g5v2 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.687e-21
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LDMV  S ++KEYI GGGFKEDVP+  EVIVQKGVA FNNQEAAEEISNHI EFIKKF
Sbjct:  255 VKFITGDLDMVYTSPHVKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAEEISNHIYEFIKKF 319

HSP 2 Score: 27.7202 bits (60), Expect = 2.687e-21
Identity = 17/36 (47.22%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G    GL  YR+  LN E   PW G K K
Sbjct:  218 VSKYEKTG-FTGGLNYYRNFNLNWELTAPWSGVKIK 252

[Close]

BLAST of GR391359 vs. TrEMBL
Match: C6TK81_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.058e-19
Identity = 48/65 (73.85%), Postives = 56/65 (86.15%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+AGELDMV  SL +KEYI GGGFKEDVP+  +VIVQKGVA FNNQEAAE+ISN+I +FI KF
Sbjct:  254 VKFIAGELDMVYTSLGIKEYIHGGGFKEDVPNLEQVIVQKGVAHFNNQEAAEDISNYIHDFINKF 318

HSP 2 Score: 23.483 bits (49), Expect = 3.058e-19
Identity = 15/36 (41.67%), Postives = 18/36 (50.00%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G    GL  YR++  N E  TPW   + K
Sbjct:  217 VSKFEKTG-FTGGLNYYRNINSNWELTTPWTRVQIK 251

[Close]

BLAST of GR391359 vs. TrEMBL
Match: Q39856_SOYBN (Epoxide hydrolase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 6.438e-18
Identity = 52/89 (58.43%), Postives = 63/89 (70.79%), Query Frame = -1

Query:  191 REIKLRMNDTMGWSKI*GGRMK----FMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 445
            R   L    T  W+   GG++K    ++ GELDMV  SLN+KEYI GGGFK+DVP+  +VIVQKGVA FNNQEAAEEI N+I +FI KF
Sbjct:  256 RNFNLNWELTAPWT---GGQIKVPVKYITGELDMVYNSLNLKEYIHGGGFKQDVPNLEQVIVQKGVAHFNNQEAAEEIDNYIYDFINKF 341

[Close]

BLAST of GR391359 vs. TrEMBL
Match: O49857_SOYBN (Epoxide hydrolase OS=Glycine max PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 6.438e-18
Identity = 52/89 (58.43%), Postives = 63/89 (70.79%), Query Frame = -1

Query:  191 REIKLRMNDTMGWSKI*GGRMK----FMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 445
            R   L    T  W+   GG++K    ++ GELDMV  SLN+KEYI GGGFK+DVP+  +VIVQKGVA FNNQEAAEEI N+I +FI KF
Sbjct:  256 RNFNLNWELTAPWT---GGQIKVPVKYITGELDMVYNSLNLKEYIHGGGFKQDVPNLEQVIVQKGVAHFNNQEAAEEIDNYIYDFINKF 341

[Close]

BLAST of GR391359 vs. TrEMBL
Match: C6TC98_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.195e-17
Identity = 51/88 (57.95%), Postives = 62/88 (70.45%), Query Frame = -1

Query:  194 REIKLRMNDTMGWSKI*GGRMK----FMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKK 445
            R   L    T  W+   GG++K    ++ GELDMV  SLN+KEYI GGGFK+DVP+  +VIVQKGVA FNNQEAAEEI N+I +FI K
Sbjct:  231 RNFNLNWELTAPWT---GGQIKVPVKYITGELDMVYNSLNLKEYIHGGGFKQDVPNLEQVIVQKGVAHFNNQEAAEEIDNYIYDFINK 315

[Close]

BLAST of GR391359 vs. TAIR peptide
Match: AT3G05600.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:1623485-1624704 REVERSE LENGTH=331)

HSP 1 Score: 62.003 bits (149), Expect = 1.609e-12
Identity = 30/64 (46.88%), Postives = 40/64 (62.50%), Query Frame = -1

Query:  194 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKK 385
            +KFM G+ DMV  +  MKEYI GGGF  DVP   E++V +    F NQE  +E++ HI +F  K
Sbjct:  260 VKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIEDAGHFVNQEKPQEVTAHINDFFTK 323

HSP 2 Score: 27.7202 bits (60), Expect = 1.609e-12
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -2

Query:  394 VSKAEWGGSLPEGLR*YRSVRLN*E*MTPWGGAKFK 501
            VSK E  G    GL  YR++ LN E   PW GAK +
Sbjct:  223 VSKFEKAG-FTGGLNYYRAMDLNWELTAPWTGAKIQ 257

[Close]

BLAST of GR391359 vs. TAIR peptide
Match: AT2G26740.1 (| Symbols: ATSEH, SEH | soluble epoxide hydrolase | chr2:11393148-11394257 REVERSE LENGTH=321)

HSP 1 Score: 60.4622 bits (145), Expect = 6.423e-10
Identity = 34/64 (53.12%), Postives = 41/64 (64.06%), Query Frame = -1

Query:  191 KFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 382
            KF+ GELD+V     +KEYI G  FKEDVP   E +V +GVA F NQE  +EI   I +FI KF
Sbjct:  258 KFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAHFINQEKPQEILQIILDFISKF 321

HSP 2 Score: 20.4014 bits (41), Expect = 6.423e-10
Identity = 8/20 (40.00%), Postives = 12/20 (60.00%), Query Frame = -2

Query:  394 YRSVRLN*E*MTPWGGAKFK 453
            YR+   N E + PW G+K +
Sbjct:  235 YRNFNRNNELLGPWVGSKIQ 254

[Close]

BLAST of GR391359 vs. TAIR peptide
Match: AT4G02340.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:1035722-1037403 FORWARD LENGTH=324)

HSP 1 Score: 56.9954 bits (136), Expect = 1.080e-9
Identity = 29/65 (44.62%), Postives = 41/65 (63.08%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+LD+       KEYI  GG K+ VP   EV+V +GV  F +QE  +E+++HI  F KKF
Sbjct:  252 VKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQEKPDEVTDHIYGFFKKF 316

HSP 2 Score: 23.0978 bits (48), Expect = 1.080e-9
Identity = 10/24 (41.67%), Postives = 14/24 (58.33%), Query Frame = -2

Query:  394 GLR*YRSVRLN*E*MTPWGGAKFK 465
            GL  YR++ L+ E   PW G + K
Sbjct:  226 GLNYYRALNLSWELTAPWTGLQIK 249

[Close]

BLAST of GR391359 vs. TAIR peptide
Match: AT2G26750.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:11395971-11397085 REVERSE LENGTH=320)

HSP 1 Score: 58.151 bits (139), Expect = 3.051e-9
Identity = 33/64 (51.56%), Postives = 40/64 (62.50%), Query Frame = -1

Query:  191 KFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 382
            KF+ GELD+V     +KEYI G  FKEDVP   E +V +GVA F NQE  +EI   I +FI  F
Sbjct:  257 KFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHFLNQEKPQEILQIILDFISTF 320

HSP 2 Score: 20.4014 bits (41), Expect = 3.051e-9
Identity = 8/20 (40.00%), Postives = 12/20 (60.00%), Query Frame = -2

Query:  394 YRSVRLN*E*MTPWGGAKFK 453
            YR+   N E + PW G+K +
Sbjct:  234 YRNFNRNNELLGPWVGSKIQ 253

[Close]

BLAST of GR391359 vs. TAIR peptide
Match: AT4G15960.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9045763-9047199 REVERSE LENGTH=375)

HSP 1 Score: 54.6842 bits (130), Expect = 6.604e-9
Identity = 28/65 (43.08%), Postives = 38/65 (58.46%), Query Frame = -1

Query:  191 MKFMAGELDMVCASLNMKEYILGGGFKEDVPDGGEVIVQKGVARFNNQEAAEEISNHI*EFIKKF 385
            +KF+ G+ D+       KEYI GGGFK DVP   E +V KG+  F ++E  + I+ HI  F  KF
Sbjct:  310 VKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKGLGHFLHEENPDVINQHIHNFFHKF 374

HSP 2 Score: 22.7126 bits (47), Expect = 6.604e-9
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = -2

Query:  394 YRSVRLN*E*MTPWGGAKFK 453
            YR++  N E   PW GAK +
Sbjct:  288 YRNIDRNWELTAPWTGAKIR 307

The following BLAST results are available for this feature:

BLAST of GR391359 vs. TrEMBL
BLAST of GR391359 vs. TAIR peptide

BLAST of GR391359 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8LPE6_CICAR	4.853e-24	83.08	Epoxide hydrolase (Fragment) OS=Cicer arietinum PE...	[more]
A2Q321_MEDTR	5.178e-23	80.00	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
A2Q324_MEDTR	1.141e-22	80.00	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
B7FJ70_MEDTR	3.271e-22	78.46	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FJM9_MEDTR	4.256e-22	78.46	Putative uncharacterized protein OS=Medicago trunc...	[more]
A2Q320_MEDTR	2.687e-21	76.92	Epoxide hydrolase OS=Medicago truncatula GN=MtrDRA...	[more]
C6TK81_SOYBN	3.058e-19	73.85	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q39856_SOYBN	6.438e-18	58.43	Epoxide hydrolase OS=Glycine max PE=2 SV=1	[more]
O49857_SOYBN	6.438e-18	58.43	Epoxide hydrolase OS=Glycine max PE=4 SV=1	[more]
C6TC98_SOYBN	3.195e-17	57.95	Putative uncharacterized protein (Fragment) OS=Gly...	[more]

BLAST of GR391359 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G05600.1	1.609e-12	46.88	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G26740.1	6.423e-10	53.13	\| Symbols: ATSEH, SEH \| soluble epoxide hydrolase ...	[more]
AT4G02340.1	1.080e-9	44.62	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G26750.1	3.051e-9	51.56	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15960.1	6.604e-9	43.08	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 50..59 score
None	No IPR available	SEG	seg	seg	coord: 10..30 score: NAcoord: 37..48 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR391359 ID=GR391359; Name=GR391359; organism=Cicer arietinum; type=EST; length=658bp
GCAGAAATTGACAGGGTATTATCAACATGGTTTTGAACCAAAAAGATATA
ATCCAATATCTCATGGCACAGTTGGGTTGTTATTACATTTAAGTGGATAG
AGTACAAGTAAATCTAACACATGCAAAACTTATAAGAATCACGAAATAGA
CTTAGCAGCATGGCTATTATTTCCTTGGAAGAACAGCTCCAAACTTTTTG
ATGAATTCCTAAATGTGATTACTGATTTCTTCTGCTGCTTCTTGATTGTT
GAAACGAGCCACTCCTTTTTGTACAATCACTTCCCCCCCGTCAGGAACAT
CTTCCTTGAATCCTCCACCCAGGATATATTCCTTCATGTTAAGTGAGGCG
CATACCATGTCTAACTCCCCCGCCATGAATTTCATCCGGCCCCCTTAAAT
TTTGCTCCACCCCATGGTGTCATTCATTCTCAATTTAATCTCACGCTTCT
ATATCACCTTAATCCTTCCGGTAAACTCCCCCCCCACTCCGCCTTTGAAA
CCAATGCTTAACTCCCTAGTTGAACTCCTGGACCGTTTGACTGTCCCACC
TTTTTTTCCACCCCCCCATCCCTTTCGTACCCCTTCCCTGTCTCTTTTTT
TAATGTTCCCCTAAATAATTTTTGCCATAATCTAGTGCCTAATTCCCCTT
TTCGTTCG

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