GR395966

EST Overview
NameGR395966
Unique NameGR395966
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length723
Libraries
Library NameType
LIBEST_024781 ICC4958 dehydration stressed root cDNA librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of GR395966 vs. SwissProt
Match: CRF1_ASPFU (Probable glycosidase crf1 OS=Aspergillus fumigatus GN=crf1 PE=1 SV=2)

HSP 1 Score: 103.605 bits (257), Expect = 1.135e-21
Identity = 49/100 (49.00%), Postives = 66/100 (66.00%), Query Frame = -1 
Query:  421 FLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSVPYPES 720
            F G  EV ++A  G G+ +  VL+SD+LDE+DWE  GGDT QVQ+NYF KGD +TYDRG + PVA P      Y+I+WT   + W I   VVR++ Y ++
Sbjct:   90 FFGKAEVVMKAAPGTGVVSSIVLESDDLDEVDWEVLGGDTTQVQTNYFGKGDTTTYDRGTYVPVATPQETFHTYTIDWTKDAVTWSIDGAVVRTLTYNDA 189
[Close]
BLAST of GR395966 vs. SwissProt
Match: CRH1_YEAST (Probable glycosidase CRH1 OS=Saccharomyces cerevisiae GN=CRH1 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.616e-18
Identity = 50/110 (45.45%), Postives = 65/110 (59.09%), Query Frame = -1 
Query:  412 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVR------SVPYPESMVH 723
            I  G LEV ++A  G GI +   LQSD+LDEID E  GGD  Q QSN+FSKGD +TYDRGEFH V  P  +   Y+++W      W +  + VR      S  YP+S ++
Sbjct:  104 IMYGKLEVILKAANGTGIVSSFYLQSDDLDEIDIEWVGGDNTQFQSNFFSKGDTTTYDRGEFHGVDTPTDKFHNYTLDWAMDKTTWYLDGESVRVLSNTSSEGYPQSPMY 213
[Close]
BLAST of GR395966 vs. SwissProt
Match: CRF2_ASPFU (Probable glycosidase crf2 OS=Aspergillus fumigatus GN=crf1 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.079e-7
Identity = 33/98 (33.67%), Postives = 53/98 (54.08%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVA--NPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            I+ G +  ++++  G G+ T  +L SD  DEID+E  G D   VQ+NY+ +G    Y+ G    V+  N  G    Y+I+W P  I W +  +V R++
Sbjct:  134 IWYGKITAKIKSSRGAGVVTGFILLSDTKDEIDYEFVGADLTNVQTNYYFQG-VLDYNHGGNASVSGGNTFGDWHEYTIDWKPDAITWSVDGEVKRTL 230
[Close]
BLAST of GR395966 vs. TrEMBL
Match: C7Z603_NECH7 (Putative uncharacterized protein OS=Nectria haematococca (strain 77-13-4 / FGSC 9596 / MPVI) GN=NECHADRAFT_66036 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 5.898e-35
Identity = 70/101 (69.31%), Postives = 79/101 (78.22%), Query Frame = -1 
Query:  421 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSVPYPES 723
            IF G ++VEVQA  GVGI T  VLQSDNLDEIDWE  GGD AQVQSNYFSKGD STYDRG FHPV NP GQ  +YSIEWTPS +NW+I   VVR++ Y ++
Sbjct:   91 IFFGRVDVEVQAAPGVGIITSAVLQSDNLDEIDWEWIGGDNAQVQSNYFSKGDISTYDRGAFHPVVNPTGQFHVYSIEWTPSFVNWIIDGTVVRTLTYADA 191

HSP 2 Score: 28.4906 bits (62), Expect = 5.898e-35
Identity = 15/51 (29.41%), Postives = 22/51 (43.14%), Query Frame = -2 
Query:  258 NGYTGVPMQIKFGTWTSDHSNRAHEASIRYVGLEDISLAFREAYPKSSIVV 410
            + +   PMQ+K GTW +   +      I   G+ D S      Y KS  +V
Sbjct:  195 SAFPQTPMQVKLGTWVAGRPDAEEGTVIWAGGMADFSQGPFSGYYKSISIV 245
[Close]
BLAST of GR395966 vs. TrEMBL
Match: Q2LC45_METAN (Cell wall glucanosyltransferase Mwg1 OS=Metarhizium anisopliae GN=Mwg1 PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 4.472e-28
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = -1 
Query:  442 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVR 723
            IF G ++V +QA  G GI T  VLQSD+LDEIDWE  G D AQVQ+NYFSKGD +TYDRG++HP+ NP G    Y++EWT   +NW+I  Q VR
Sbjct:   88 IFFGRVDVVMQAAPGAGIITSAVLQSDDLDEIDWEFVGSDNAQVQTNYFSKGDTTTYDRGKYHPIGNPTGSFHTYTVEWTSKQVNWMIDGQSVR 181

HSP 2 Score: 30.4166 bits (67), Expect = 4.472e-28
Identity = 18/45 (40.00%), Postives = 21/45 (46.67%), Query Frame = -2 
Query:  258 PMQIKFGTWTSDHSNRAHEASIRYVGLEDISLAFREAYPKSSIVV 392
            PMQIK GTW +   N          G  D S A  +AY KS  +V
Sbjct:  196 PMQIKLGTWCAGGKNSPEGTRQWAGGFTDFSKAPFDAYYKSISIV 240
[Close]
BLAST of GR395966 vs. TrEMBL
Match: C7Z5D5_NECH7 (Putative uncharacterized protein (Fragment) OS=Nectria haematococca (strain 77-13-4 / FGSC 9596 / MPVI) GN=NECHADRAFT_28207 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 5.198e-26
Identity = 58/96 (60.42%), Postives = 70/96 (72.92%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G ++V VQA  G GI T  VLQSD+LDEIDWE  GGD AQVQSNYFSKGD +TYDR  +HPV+ P      YS+EWT + I+W+I D VVR++
Sbjct:   91 IFFGRVDVVVQAAQGQGIVTSAVLQSDDLDEIDWEWVGGDNAQVQSNYFSKGDTTTYDRAVYHPVSAPLTSTHKYSVEWTSTKIDWLIDDAVVRTL 186

HSP 2 Score: 25.7942 bits (55), Expect = 5.198e-26
Identity = 17/54 (31.48%), Postives = 23/54 (42.59%), Query Frame = -2 
Query:  258 NGYTGVPMQIKFGTWTSDHSNR---AHEASIRYVGLEDISLAFREAYPKSSIVV 410
            + +   PMQIK GTW +   N      E +  Y   +D       AY KS  +V
Sbjct:  195 SAFPQTPMQIKMGTWVAGGKNSNAGTREWAGGYTNFDDAPFT---AYYKSITIV 245
[Close]
BLAST of GR395966 vs. TrEMBL
Match: Q2LC44_METAN (Cell wall glucanosyltransferase Mwg2 OS=Metarhizium anisopliae GN=Mwg2 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.528e-25
Identity = 55/96 (57.29%), Postives = 68/96 (70.83%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G  EV +QA  G GI T  VLQSD+LDEIDWE  G D A+VQ+NYFSKG+ STYDRG+FH V+NP G    Y+IEWT + + W I  + VR++
Sbjct:   92 IFFGKFEVHIQAAPGAGIVTSAVLQSDDLDEIDWEFIGSDNARVQTNYFSKGNTSTYDRGKFHAVSNPTGSAHRYTIEWTKTHLVWSIDGEPVRTL 187

HSP 2 Score: 27.7202 bits (60), Expect = 2.528e-25
Identity = 13/37 (35.14%), Postives = 21/37 (56.76%), Query Frame = -2 
Query:  315 RAWFTNGYTGVPMQIKFGTWTSDHSNRAHEASIRYVG 425
            +A  ++G+   PMQIK GTW +     A + +I + G
Sbjct:  193 KAGTSSGFPQTPMQIKLGTWVAGKPGSA-QGTIDWAG 228
[Close]
BLAST of GR395966 vs. TrEMBL
Match: B2ARI3_PODAN (Predicted CDS Pa_4_7400 OS=Podospora anserina PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 4.262e-25
Identity = 54/96 (56.25%), Postives = 68/96 (70.83%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G ++V ++A  G G+ T  VLQSD+LDEIDWE  GGD AQVQ NYFSKG   TYDRG F PVA+P G    Y+I+WT   ++W+I  QVVR++
Sbjct:   76 IFFGQVDVVLRAAPGPGVVTSFVLQSDDLDEIDWEWLGGDNAQVQHNYFSKGCTETYDRGGFSPVADPIGAFHTYTIKWTSEQLDWIIDGQVVRTL 171

HSP 2 Score: 30.4166 bits (67), Expect = 4.262e-25
Identity = 18/51 (35.29%), Postives = 27/51 (52.94%), Query Frame = -2 
Query:  258 GYTGVPMQIKFGTWTSDHSNRAHEASIRYV-GLEDISLAFREAYPKSSIVV 407
            GY   PMQIK GTW +   + A + +I +  GL D S    + Y +S  ++
Sbjct:  181 GYPQSPMQIKLGTWVAGRKD-APQGTIEWAGGLADFSNGPSDGYYQSLKII 230
[Close]
BLAST of GR395966 vs. TrEMBL
Match: Q7S222_NEUCR (Putative uncharacterized protein OS=Neurospora crassa GN=NCU05974 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 9.389e-25
Identity = 53/96 (55.21%), Postives = 68/96 (70.83%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G ++V V+A  G G+ T  VLQSD+LDEIDWE  G DT QVQ+NYFSKG   TYDRG F  VANP  +   Y+I+WTP+ ++W+I   VVR++
Sbjct:   88 IFFGQVDVTVRAAFGKGVVTSFVLQSDDLDEIDWEWIGSDTTQVQTNYFSKGCTETYDRGGFSTVANPQNEFHTYTIKWTPTQLDWIIDGAVVRTL 183

HSP 2 Score: 28.1054 bits (61), Expect = 9.389e-25
Identity = 12/31 (38.71%), Postives = 18/31 (58.06%), Query Frame = -2 
Query:  315 GYTGVPMQIKFGTWTSDHSNRAHEASIRYVG 407
            G+   PMQIK GTW +   + A + +I + G
Sbjct:  193 GFPQTPMQIKLGTWVAGRKDAA-QGTIEWAG 222
[Close]
BLAST of GR395966 vs. TrEMBL
Match: D1ZEZ0_SORMA (Whole genome shotgun sequence assembly, scaffold_24 OS=Sordaria macrospora GN=SMAC_07003 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 7.724e-24
Identity = 53/96 (55.21%), Postives = 68/96 (70.83%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G ++V V+A  G GI T  VLQSD+LDEIDWE  G D  QVQ+NYFS+G   TYDRG F PVANP  +   Y+I+WTP+ ++W+I   VVR++
Sbjct:   88 IFFGQVDVTVRAAFGKGIVTSFVLQSDDLDEIDWEWIGSDHTQVQTNYFSQGCTETYDRGGFSPVANPQDEFHTYTIKWTPTQLDWIIDGTVVRTL 183

HSP 2 Score: 26.1794 bits (56), Expect = 7.724e-24
Identity = 11/32 (34.38%), Postives = 18/32 (56.25%), Query Frame = -2 
Query:  315 NGYTGVPMQIKFGTWTSDHSNRAHEASIRYVG 410
            + +   PMQIK GTW +   + A + +I + G
Sbjct:  192 SAFPQTPMQIKLGTWVAGRKDAA-QGTIEWAG 222
[Close]
BLAST of GR395966 vs. TrEMBL
Match: Q71SX2_9HYPO (Ice nucleation protein OS=Gibberella acuminata PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 6.331e-23
Identity = 51/96 (53.12%), Postives = 70/96 (72.92%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            +F G +++ ++A  G GI T  VLQSD+LDEID+E  GGD AQVQ+NYFSKGD +TYDR  +HPV+ P      YS+EWT + ++W+I D VVR++
Sbjct:   91 LFFGRVDIVMEAAKGQGIVTSVVLQSDDLDEIDFEMVGGDDAQVQTNYFSKGDTTTYDRAIYHPVSAPLSTTHKYSVEWTSTKVDWLIDDVVVRTL 186

HSP 2 Score: 26.1794 bits (56), Expect = 6.331e-23
Identity = 14/45 (31.11%), Postives = 20/45 (44.44%), Query Frame = -2 
Query:  258 PMQIKFGTWTSDHSNRAHEASIRYVGLEDISLAFREAYPKSSIVV 392
            PMQ+K GTW +   N          G  D   A  +AY +S  ++
Sbjct:  202 PMQLKLGTWVAGGKNSNEGTREWAGGYTDFKEAPFDAYYRSITII 246
[Close]
BLAST of GR395966 vs. TrEMBL
Match: C9STC9_VERA1 (Ice nucleation protein OS=Verticillium albo-atrum (strain VaMs.102) GN=VDBG_08154 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.401e-22
Identity = 55/96 (57.29%), Postives = 63/96 (65.62%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G LE  VQA    GI T  VLQS +LDEIDWE  GGD A VQ+NYFSKGD +TYDRG  H VANP  Q   Y I W  + I+W+I   VVR++
Sbjct:   89 IFFGELECNVQAANMQGIVTSIVLQSKDLDEIDWEWTGGDNANVQTNYFSKGDTTTYDRGGKHAVANPLNQFHKYGIVWNQNKIDWIIDGTVVRTL 184

HSP 2 Score: 26.9498 bits (58), Expect = 1.401e-22
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  348 YTGVPMQIKFGTWTSDHSN 404
            Y   PMQIK GTW + + N
Sbjct:  193 YPQTPMQIKIGTWPAGNKN 211
[Close]
BLAST of GR395966 vs. TrEMBL
Match: Q6QUX9_VERDA (Allergen rAsp f9-like protein OS=Verticillium dahliae PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 4.007e-22
Identity = 55/96 (57.29%), Postives = 62/96 (64.58%), Query Frame = -1 
Query:  436 IFLGGLEVEVQA*IGVGISTRRVLQSDNLDEIDWEGPGGDTAQVQSNYFSKGDGSTYDRGEFHPVANPPGQCEIYSIEWTPSPINWVIVDQVVRSV 723
            IF G LE  VQA    GI T  VLQS +LDEIDWE  GGD A VQ+NYFSKGD +TYDRG  H VANP  Q   Y I W  + I+W I   VVR++
Sbjct:   89 IFFGELECTVQAANMQGIVTSIVLQSKDLDEIDWEWTGGDNANVQTNYFSKGDTTTYDRGGKHAVANPLNQFHKYGIVWNQNKIDWTIDGAVVRTL 184

HSP 2 Score: 26.9498 bits (58), Expect = 4.007e-22
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  348 YTGVPMQIKFGTWTSDHSN 404
            Y   PMQIK GTW + + N
Sbjct:  193 YPQTPMQIKIGTWPAGNKN 211
The following BLAST results are available for this feature:
BLAST of GR395966 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
CRF1_ASPFU1.135e-2149.00Probable glycosidase crf1 OS=Aspergillus fumigatus... [more]
CRH1_YEAST7.616e-1845.45Probable glycosidase CRH1 OS=Saccharomyces cerevis... [more]
CRF2_ASPFU2.079e-733.67Probable glycosidase crf2 OS=Aspergillus fumigatus... [more]
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BLAST of GR395966 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C7Z603_NECH75.898e-3569.31Putative uncharacterized protein OS=Nectria haemat... [more]
Q2LC45_METAN4.472e-2858.51Cell wall glucanosyltransferase Mwg1 OS=Metarhiziu... [more]
C7Z5D5_NECH75.198e-2660.42Putative uncharacterized protein (Fragment) OS=Nec... [more]
Q2LC44_METAN2.528e-2557.29Cell wall glucanosyltransferase Mwg2 OS=Metarhiziu... [more]
B2ARI3_PODAN4.262e-2556.25Predicted CDS Pa_4_7400 OS=Podospora anserina PE=4... [more]
Q7S222_NEUCR9.389e-2555.21Putative uncharacterized protein OS=Neurospora cra... [more]
D1ZEZ0_SORMA7.724e-2455.21Whole genome shotgun sequence assembly, scaffold_2... [more]
Q71SX2_9HYPO6.331e-2353.13Ice nucleation protein OS=Gibberella acuminata PE=... [more]
C9STC9_VERA11.401e-2257.29Ice nucleation protein OS=Verticillium albo-atrum ... [more]
Q6QUX9_VERDA4.007e-2257.29Allergen rAsp f9-like protein OS=Verticillium dahl... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000757Glycoside hydrolase, family 16PFAMPF00722Glyco_hydro_16coord: 4..89
score: 3.8
IPR008985Concanavalin A-like lectin/glucanaseSUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 2..85
score: 4.7
IPR013320Concanavalin A-like lectin/glucanase, subgroupGENE3D2.60.120.200no descriptioncoord: 4..90
score: 1.7

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR395966 ID=GR395966; Name=GR395966; organism=Cicer arietinum; type=EST; length=723bp
GCGGGTTTTCCTCTGATCAGGTAGGAGTACCCGCTGACCTTAAGCATATC
AATCAGCAGAGGAAAAGAAACCAGCCGGGATTGCCCGGGGAACCGCTAGT
GAATCGGCAACACCTCAAATTTCAAATCTGGTTATCCGGTCCGAATTGTA
ATTTGCAGAGGATACTTTTGATGCGGTGCCTTCCGACTTCCCTGGAGCGC
GACGCCATTGAGCGTGAGAACCCCCTCTGGTTGGATGCCAAATCTCTGTA
CAGTTCCTACCACGATACTGCTCTTTGGGTAAGCTTCTCTGAACGCTAGG
GATATGTCTTCTAAACCTACATACCGGATGGAGGCCTCATGGGCACGATT
AGAGTGATCGGAAGTCCAAGTTCCGAACTTGATCTGCATGGGCACTCCGG
TATAACCATTGGTGAACCATGCTCTCAGGGTAGGGAACGCTTCGGACCAC
TTGGTCGACGATCACCCAGTTGATGGGGGAAGGCGTCCACTCTATGGAGT
ATATCTCGCATTGACCTGGTGGGTTGGCGACCGGATGGAATTCCCCGCGA
TCATATGTTGACCCATCTCCCTTGCTGAAATAATTTGACTGAACTTGAGC
GGTATCGCCTCCAGGCCCCTCCCAATCAATCTCATCCAGGTTATCGGACT
GAAGAACACGCCTAGTGCTGATGCCTACACCAATTTAGGCTTGCACCTCC
ACTTCCAAACCGCCCAAAAAAAT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR000757GH16
IPR008985Concanavalin A-like lectin/glucanase
IPR013320ConA-like_dom_sf