GR400051

EST Overview


Name	GR400051
Unique Name	GR400051
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	425

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX3_PEA (Seed lipoxygenase-3 OS=Pisum sativum GN=LOX1.3 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.008e-10
Identity = 31/55 (56.36%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FG  LAEI++KL++R  D+ L+N +GPV MPY LLYPS+  G TF G+P+ ISI
Sbjct:  807 KFGNKLAEIEKKLTQRNNDEKLRNRHGPVEMPYTLLYPSSKEGLTFRGIPNSISI 861

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX1_LENCU (Lipoxygenase OS=Lens culinaris GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.008e-10
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK LAEI++KL++R  D++L+N  GPV MPY LLYPS+  G T  G+P+ ISI
Sbjct:  812 RFGKKLAEIEEKLTQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI 866

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX4_SOYBN (Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.830e-10
Identity = 31/55 (56.36%), Postives = 39/55 (70.91%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK L EI++KL  +  D+TL+N  GP  MPY LLYPS+  G TF G+P+ ISI
Sbjct:  799 RFGKKLEEIEKKLIEKNKDETLRNRYGPAKMPYTLLYPSSEEGLTFRGIPNSISI 853

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX3_SOYBN (Seed lipoxygenase-3 OS=Glycine max GN=LOX1.3 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.901e-9
Identity = 31/55 (56.36%), Postives = 38/55 (69.09%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG  LA+I+ KLS R  D+ L+N  GPV MPY LL PS+  G TF G+P+ ISI
Sbjct:  803 RFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI 857

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOXB_PHAVU (Lipoxygenase (Fragment) OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.531e-9
Identity = 28/49 (57.14%), Postives = 36/49 (73.47%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGV 150
            RFGK LAEI++KL ++  D+TL+N  GP  MPY LLYPS+  G TF G+
Sbjct:  693 RFGKKLAEIEKKLVQKNNDETLRNRTGPAKMPYTLLYPSSEEGLTFRGI 741

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX1_SOYBN (Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 6.115e-8
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLK-NLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FG  L EI++KL RR  D +L+ N  GPV +PY LLYPS+  G TF G+P+ ISI
Sbjct:  784 KFGNKLKEIEEKLVRRNNDPSLQGNRLGPVQLPYTLLYPSSEEGLTFRGIPNSISI 839

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX2_PEA (Seed lipoxygenase-2 OS=Pisum sativum GN=LOX1.2 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.779e-7
Identity = 27/55 (49.09%), Postives = 38/55 (69.09%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FG  LAEI+ KL+ +  D +L +  GPV +PY LL+PS+  G TF G+P+ ISI
Sbjct:  810 KFGNKLAEIEAKLTNKNNDPSLYHRVGPVQLPYTLLHPSSKEGLTFRGIPNSISI 864

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOXA_PHAVU (Lipoxygenase 1 OS=Phaseolus vulgaris GN=LOXA PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.035e-7
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPS-NV*GSTFIGVPHIISI 168
            RFG+ L EI++K+  R  D +L+N NGPV MPY +L P+    G TF G+P+ ISI
Sbjct:  807 RFGQKLKEIEEKIMGRNKDSSLRNRNGPVKMPYTVLLPTCEDEGLTFRGIPNSISI 862

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX1_SOLTU (Lipoxygenase 1 OS=Solanum tuberosum GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.035e-7
Identity = 25/55 (45.45%), Postives = 39/55 (70.91%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK L +I++++ +R  D+ L N +GPVN PY LL+P++  G T  G+P+ +SI
Sbjct:  807 RFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYTLLFPTSEGGLTGKGIPNSVSI 861

[Close]

BLAST of GR400051 vs. SwissProt
Match: LOX1_ARATH (Lipoxygenase 1 OS=Arabidopsis thaliana GN=LOX1 PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.761e-7
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FG+ + EI++ +  R  D+TLKN  G V MPY LL+PS+  G T  G+P+ +SI
Sbjct:  805 KFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q9FQF9_PHAVU (Lipoxygenase OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.371e-10
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK LAEI++KL ++  D+TL+N  GP  MPY LLYPS+  G TF G+P+ ISI
Sbjct:  820 RFGKKLAEIEEKLVKKNNDETLRNRTGPAKMPYTLLYPSSEEGLTFRGIPNSISI 874

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q43817_PEA (Lipoxygenase OS=Pisum sativum GN=LoxG PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.015e-10
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FGK LAEI+QKL +R  D++L+N  GPV MPY LLYPS+  G TF G+P+ +SI
Sbjct:  814 KFGKKLAEIEQKLVQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTFRGIPNSVSI 868

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q9M684_PHAVU (Lipoxygenase (Fragment) OS=Phaseolus vulgaris GN=LOX4 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.162e-10
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK LAEI++KL ++  D+TL+N  GP  MPY LLYPS+  G TF G+P+ ISI
Sbjct:  802 RFGKKLAEIEKKLVQKNNDETLRNRTGPAKMPYTLLYPSSEEGLTFRGIPNSISI 856

[Close]

BLAST of GR400051 vs. TrEMBL
Match: B3TDK9_SOYBN (Lipoxygenase OS=Glycine max PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.162e-10
Identity = 32/55 (58.18%), Postives = 41/55 (74.55%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGK L EI+QKL ++  D+TL+N +GP  MPY LLYPS+  G TF G+P+ ISI
Sbjct:  802 RFGKKLQEIEQKLIQKNKDETLRNRSGPAKMPYTLLYPSSEEGLTFRGIPNSISI 856

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q43446_SOYBN (Lipoxygenase OS=Glycine max GN=vlxB PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.563e-9
Identity = 32/55 (58.18%), Postives = 40/55 (72.73%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFGKNL EI++KL  +  D+TL+N  GP  MPY LLYPS+  G TF G+P+ ISI
Sbjct:  799 RFGKNLEEIEKKLIEKNNDETLRNRYGPAKMPYTLLYPSSEEGLTFRGIPNSISI 853

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q9ZSQ2_PEA (Lipoxygenase (Fragment) OS=Pisum sativum GN=LoxN3 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.041e-9
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG+ LAEI+QKL++R  D++L+N  GPV MPY LLYPS+  G T  G+P+ ISI
Sbjct:  438 RFGRKLAEIEQKLTQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI 492

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q14ST9_PEA (Lipoxygenase OS=Pisum sativum GN=loxN2 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.041e-9
Identity = 32/55 (58.18%), Postives = 40/55 (72.73%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG  LAEI++KL  R  D+TL+N  GPV MPY +LYPS+  G TF G+P+ ISI
Sbjct:  772 RFGSKLAEIEKKLIERNNDETLRNRYGPVKMPYTILYPSSEAGLTFRGIPNSISI 826

[Close]

BLAST of GR400051 vs. TrEMBL
Match: Q14ST8_PEA (Lipoxygenase OS=Pisum sativum GN=loxN2 PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.041e-9
Identity = 32/55 (58.18%), Postives = 40/55 (72.73%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG  LAEI++KL  R  D+TL+N  GPV MPY +LYPS+  G TF G+P+ ISI
Sbjct:  772 RFGSKLAEIEKKLIERNNDETLRNRYGPVKMPYTILYPSSEAGLTFRGIPNSISI 826

[Close]

BLAST of GR400051 vs. TrEMBL
Match: B7Z177_PEA (Lipoxygenase OS=Pisum sativum GN=loxN3 PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.041e-9
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG+ LAEI+QKL++R  D++L+N  GPV MPY LLYPS+  G T  G+P+ ISI
Sbjct:  812 RFGRKLAEIEQKLTQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI 866

[Close]

BLAST of GR400051 vs. TrEMBL
Match: B7Z176_PEA (Lipoxygenase OS=Pisum sativum GN=loxN3 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.041e-9
Identity = 32/55 (58.18%), Postives = 42/55 (76.36%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            RFG+ LAEI+QKL++R  D++L+N  GPV MPY LLYPS+  G T  G+P+ ISI
Sbjct:  812 RFGRKLAEIEQKLTQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI 866

[Close]

BLAST of GR400051 vs. TAIR peptide
Match: AT1G55020.1 (| Symbols: LOX1, ATLOX1 | lipoxygenase 1 | chr1:20525798-20530143 FORWARD LENGTH=859)

HSP 1 Score: 52.7582 bits (125), Expect = 8.405e-8
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 1

Query:    4 RFGKNLAEIDQKLSRRIYDDTLKNLNGPVNMPYPLLYPSNV*GSTFIGVPHIISI 168
            +FG+ + EI++ +  R  D+TLKN  G V MPY LL+PS+  G T  G+P+ +SI
Sbjct:  805 KFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

The following BLAST results are available for this feature:

BLAST of GR400051 vs. SwissProt
BLAST of GR400051 vs. TrEMBL
BLAST of GR400051 vs. TAIR peptide

BLAST of GR400051 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LOX3_PEA	1.008e-10	56.36	Seed lipoxygenase-3 OS=Pisum sativum GN=LOX1.3 PE=...	[more]
LOX1_LENCU	1.008e-10	58.18	Lipoxygenase OS=Lens culinaris GN=LOX1.1 PE=2 SV=1	[more]
LOX4_SOYBN	3.830e-10	56.36	Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1	[more]
LOX3_SOYBN	1.901e-9	56.36	Seed lipoxygenase-3 OS=Glycine max GN=LOX1.3 PE=1 ...	[more]
LOXB_PHAVU	5.531e-9	57.14	Lipoxygenase (Fragment) OS=Phaseolus vulgaris PE=2...	[more]
LOX1_SOYBN	6.115e-8	53.57	Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 ...	[more]
LOX2_PEA	1.779e-7	49.09	Seed lipoxygenase-2 OS=Pisum sativum GN=LOX1.2 PE=...	[more]
LOXA_PHAVU	3.035e-7	50.00	Lipoxygenase 1 OS=Phaseolus vulgaris GN=LOXA PE=3 ...	[more]
LOX1_SOLTU	3.035e-7	45.45	Lipoxygenase 1 OS=Solanum tuberosum GN=LOX1.1 PE=2...	[more]
LOX1_ARATH	6.761e-7	45.45	Lipoxygenase 1 OS=Arabidopsis thaliana GN=LOX1 PE=...	[more]

BLAST of GR400051 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FQF9_PHAVU	5.371e-10	58.18	Lipoxygenase OS=Phaseolus vulgaris PE=3 SV=1	[more]
Q43817_PEA	7.015e-10	58.18	Lipoxygenase OS=Pisum sativum GN=LoxG PE=2 SV=1	[more]
Q9M684_PHAVU	9.162e-10	58.18	Lipoxygenase (Fragment) OS=Phaseolus vulgaris GN=L...	[more]
B3TDK9_SOYBN	9.162e-10	58.18	Lipoxygenase OS=Glycine max PE=3 SV=1	[more]
Q43446_SOYBN	1.563e-9	58.18	Lipoxygenase OS=Glycine max GN=vlxB PE=1 SV=1	[more]
Q9ZSQ2_PEA	2.041e-9	58.18	Lipoxygenase (Fragment) OS=Pisum sativum GN=LoxN3 ...	[more]
Q14ST9_PEA	2.041e-9	58.18	Lipoxygenase OS=Pisum sativum GN=loxN2 PE=2 SV=1	[more]
Q14ST8_PEA	2.041e-9	58.18	Lipoxygenase OS=Pisum sativum GN=loxN2 PE=3 SV=1	[more]
B7Z177_PEA	2.041e-9	58.18	Lipoxygenase OS=Pisum sativum GN=loxN3 PE=3 SV=1	[more]
B7Z176_PEA	2.041e-9	58.18	Lipoxygenase OS=Pisum sativum GN=loxN3 PE=2 SV=1	[more]

BLAST of GR400051 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G55020.1	8.405e-8	45.45	\| Symbols: LOX1, ATLOX1 \| lipoxygenase 1 \| chr1:20...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 38..46 score
None	No IPR available	SEG	seg	seg	coord: 56..67 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR400051 ID=GR400051; Name=GR400051; organism=Cicer arietinum; type=EST; length=425bp
CTTCGGTTTGGAAAGAATCTGGCTGAAATTGATCAAAAGCTCTCTCGAAG
GATCTATGATGATACCCTTAAGAATCTGAATGGACCTGTGAATATGCCAT
ACCCTCTTCTTTATCCTTCTAATGTATAAGGATCGACCTTTATAGGCGTT
CCCCATATTATCTCTATTTAAAGAAGGGCTTGGGTGGAGCGTTTCTACGA
CGACTATGATTAACCGATGACAAGGATCTCGATTCCCTCATGGAGGTGGA
GATAAATGCTCCCATTTTGGGGGTCACATTAACTAGCTCAGTTGTTTTTC
GGTTCAATTTTAAGTTCCCGTGTTTGTGTGTTGTTGTATGTGTCTGTATC
CATGATATCATATTTTCTTTATAACAAAGTGGAAGAAGAAAGTTATTCTG
GACGGTTTTATCCGTTTATCTTGTT

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