GR408666

EST Overview


Name	GR408666
Unique Name	GR408666
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	812

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_CHICK (Cytosolic purine 5'-nucleotidase OS=Gallus gallus GN=NT5C2 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.742e-24
Identity = 68/171 (39.77%), Postives = 96/171 (56.14%), Query Frame = 3

Query:  195 SVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLV-RDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            + D+P N  G   + +     H        ++F NRSL M+ I   GFDMDYTLA YK   +ESL +  T+++LV  +GYP ELLN+ ++  +  RGLV D   GN+LK+D +  + V  HGF  L   +    Y N  + RD  D   + +++TLF+L E YL A LVDF
Sbjct:   12 AADLPANMDGHALKKYRREAYH--------RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLV-SIGYPHELLNFVYDPAFPTRGLVFDTHYGNLLKVDAYGNLLVCAHGFNFLRGPETRDQYPNKFIQRDDTDR--FYILNTLFNLPETYLLACLVDF 171

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_XENTR (Cytosolic purine 5'-nucleotidase OS=Xenopus tropicalis GN=nt5c2 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.275e-24
Identity = 67/171 (39.18%), Postives = 96/171 (56.14%), Query Frame = 3

Query:  195 SVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLV-RDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            + D+P N  G   + +     H        ++F NRSL M+ I   GFDMDYTLA YK   +ESL +  T+++LV  +GYP ELLN+ ++  +  RGLV D   GN+LK+D +  + V  HGF  +   +    Y N  + RD  D   + +++TLF+L E YL A LVDF
Sbjct:   12 AADLPANMDGHALKKYRREAYH--------RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLV-SIGYPQELLNFVYDPTFPTRGLVFDSTYGNLLKVDAYGNILVCAHGFNFMRGPEIREQYPNKFIQRDDTDR--FYILNTLFNLPETYLLACLVDF 171

[Close]

BLAST of GR408666 vs. SwissProt
Match: 5NTC_XENLA (Cytosolic purine 5'-nucleotidase OS=Xenopus laevis GN=nt5c2 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.129e-23
Identity = 61/148 (41.22%), Postives = 90/148 (60.81%), Query Frame = 3

Query:  264 FKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLV-RDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            ++ +   ++F NRSL M+ I   GFDMDYTLA YK   +ESL +  T+++LV  +GYP ELLN+ ++  +  RGLV D   GN++K+D +  + V  HGF  +   +    Y N  + RD  D   + +++TLF+L E YL A LVDF
Sbjct:   27 YRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLV-SIGYPQELLNFVYDPTFPTRGLVFDSTYGNLIKVDAYGNILVCAHGFNFMRGLETREQYPNKFIQRDDTDR--FYILNTLFNLPETYLLACLVDF 171

[Close]

BLAST of GR408666 vs. SwissProt
Match: 5NTC_PONAB (Cytosolic purine 5'-nucleotidase OS=Pongo abelii GN=NT5C2 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.129e-23
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 3

Query:  264 FKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            ++ +   ++F NRSL M+ I   GFDMDYTLA YK   +ESL ++ T+++LV  +GYP ELL+++++  +  RGLV D   GN+LK+D +  + V  HGF  +   +    Y N  ++   D   + +++TLF+L E YL A LVDF
Sbjct:   27 YRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFYILNTLFNLPETYLLACLVDF 171

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_MOUSE (Cytosolic purine 5'-nucleotidase OS=Mus musculus GN=Nt5c2 PE=1 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 1.129e-23
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 3

Query:  264 FKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            ++ +   ++F NRSL M+ I   GFDMDYTLA YK   +ESL ++ T+++LV  +GYP ELL+++++  +  RGLV D   GN+LK+D +  + V  HGF  +   +    Y N  ++   D   + +++TLF+L E YL A LVDF
Sbjct:   27 YRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFYILNTLFNLPETYLLACLVDF 171

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_HUMAN (Cytosolic purine 5'-nucleotidase OS=Homo sapiens GN=NT5C2 PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.129e-23
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 3

Query:  264 FKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            ++ +   ++F NRSL M+ I   GFDMDYTLA YK   +ESL ++ T+++LV  +GYP ELL+++++  +  RGLV D   GN+LK+D +  + V  HGF  +   +    Y N  ++   D   + +++TLF+L E YL A LVDF
Sbjct:   27 YRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFYILNTLFNLPETYLLACLVDF 171

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_BOVIN (Cytosolic purine 5'-nucleotidase OS=Bos taurus GN=NT5C2 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.129e-23
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 3

Query:  264 FKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            ++ +   ++F NRSL M+ I   GFDMDYTLA YK   +ESL ++ T+++LV  +GYP ELL+++++  +  RGLV D   GN+LK+D +  + V  HGF  +   +    Y N  ++   D   + +++TLF+L E YL A LVDF
Sbjct:   27 YRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFYILNTLFNLPETYLLACLVDF 171

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BLAST of GR408666 vs. SwissProt
Match: 5NTC_DICDI (Cytosolic purine 5'-nucleotidase OS=Dictyostelium discoideum GN=nt5c2 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.526e-20
Identity = 59/152 (38.82%), Postives = 83/152 (54.61%), Query Frame = 3

Query:  282 KQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLV-RDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILERSRG 734
            +++F NR + +  I   GFDMDYTLA Y    FE LAY   + KL+ D+GYP  +    ++  +  RGL LD++ GN+LK+D    + +  HG   LSK      Y +  V  D      + L++TLF+L EA L+A LVD     LER  G
Sbjct:  103 RRVFVNRDIKLDRIEFFGFDMDYTLAVYNSPDFEELAYDMVLDKLI-DIGYPKSIRKLKYDPNFPTRGLFLDRELGNLLKIDSFGNIIICVHGRTTLSKNRTAEFYPSMRVSSDEIARNRFYLLNTLFTLPEACLYADLVDH----LERESG 249

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BLAST of GR408666 vs. SwissProt
Match: NT5D4_HUMAN (5'-nucleotidase domain-containing protein 4 OS=Homo sapiens GN=NT5DC4 PE=2 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 4.439e-20
Identity = 55/139 (39.57%), Postives = 86/139 (61.87%), Query Frame = 3

Query:  288 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            IF NRSL +  I   GFDMDYTLA YK   +E+L ++  +++LV  +GYP E+L ++++  +  R LV D+  GN+LK+D H  V +  +GF  LS+ +    Y +  ++   D   + +++ LF+L E YL+A LVDF
Sbjct:    5 IFVNRSLALGKIRCFGFDMDYTLAAYKSPAYEALTFELLLERLVC-IGYPHEILRYTYDPTFPTRRLVFDELYGNLLKVDAHGNVLLGAYGFTFLSEAEIWSFYPSKFIQRD-DLQCFYILNMLFNLPETYLYACLVDF 141

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BLAST of GR408666 vs. SwissProt
Match: NT5D_DICDI (5'-nucleotidase domain-containing protein DDB_G0275467 OS=Dictyostelium discoideum GN=DDB_G0275467 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.811e-12
Identity = 46/147 (31.29%), Postives = 74/147 (50.34%), Query Frame = 3

Query:  282 KQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVA--YHGFRELSKEDKVGTYGNTLVR----DSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            + +F N  L ++ I   GFD DYTLA Y  +  + L Y   +  LV +  YP  L    ++  + +RGL  D   G ++K+D    ++    YHG R L+KE+ +  YG+  ++    DSF +P    +  +F L EA L A  + +
Sbjct:  146 QDVFINSELKLEEIDVFGFDYDYTLANYGDQV-QHLIYDLAMSHLVDEQKYPMALKEIKYDPTFAIRGLHFDVNHGLLMKLDYLNNIQAGAIYHGRRPLTKEEVIQIYGSMQLKRLYCDSFLKP----MSDIFCLPEACLIANTIQY 287

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BLAST of GR408666 vs. TrEMBL
Match: B9IBG2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_573421 PE=4 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 2.013e-79
Identity = 150/218 (68.81%), Postives = 176/218 (80.73%), Query Frame = 3

Query:  126 GTNQLHSQYSMDENHDAGIHDPR--SVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILERSRGR-----*YARMYK 758
            G NQLH  Y MD+   A + D R  S  +   D  ++P IWSS      + ++GK+IFCNRSLNM+NI+AVGFDMDYTLAQYKPETFE LAY+GTI+KLV +LGYP ELL W+FNWKYMVRGLV+DKKRGNILKMDRHKYVKVAYHGFREL+KE+KVGTYGNTL+RD+FDEPDYALIDTLFSLAEAYLF+QLVD+    ++++ GR      YARMYK
Sbjct:   13 GVNQLHMSYKMDKGQKAVLADQRLSSELMESRDGVQEPCIWSSPEGGKDEAELGKRIFCNRSLNMRNIIAVGFDMDYTLAQYKPETFEKLAYEGTIEKLVSNLGYPEELLGWTFNWKYMVRGLVVDKKRGNILKMDRHKYVKVAYHGFRELTKEEKVGTYGNTLIRDAFDEPDYALIDTLFSLAEAYLFSQLVDY----MDKNPGRVPARVDYARMYK 226

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BLAST of GR408666 vs. TrEMBL
Match: B9R7U4_RICCO (Cytosolic purine 5-nucleotidase, putative OS=Ricinus communis GN=RCOM_1594360 PE=4 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 1.442e-77
Identity = 150/184 (81.52%), Postives = 159/184 (86.41%), Query Frame = 3

Query:  210 GNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILER-SRGR*YARMYK 758
            G D  ++P IWSS PE G    IGKQIFCNRSLNMKNI+AVGFDMDYTLAQYKPETFESLAY GTIKKLV+DLGYP ELL WSF+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRE+SKE+KV TYGNTL+RDSFDEPDYALIDTLFSLAEAYLFAQLV FK     +   G  YARMYK
Sbjct:   15 GADGVQEPCIWSS-PEGGCDIKIGKQIFCNRSLNMKNILAVGFDMDYTLAQYKPETFESLAYGGTIKKLVHDLGYPEELLEWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFREMSKEEKVHTYGNTLIRDSFDEPDYALIDTLFSLAEAYLFAQLVTFKDNNPAKIPEGIGYARMYK 197

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BLAST of GR408666 vs. TrEMBL
Match: D7KSL6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_895388 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 7.158e-77
Identity = 161/255 (63.14%), Postives = 185/255 (72.55%), Query Frame = 3

Query:    3 FSFSLSNFSPQLVAPLLSSRLCFAFGISVRTSRPFANIASPGTNQLHSQYSMDENHDAGIHDP--RSVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILER-SRGR*YARMYK 758
            FS  +S FSP+        R     G      R F +  + GT+QL  + SM++     + +   +   +   D  + P  WSS PE G K DI  QIFCNRSLNMKNI+AVGFDMDYTLAQYK ETFESLAY+GT++KLVYDLGYP+ELL W+F+W YMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKV  YG++LVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFK    E+  +   YARMYK
Sbjct:   62 FSLCVSTFSPRF-------RFHHFPGKFPAPRREFLH--TTGTSQLCFKSSMEDQKPTALSENALQFHTVGSRDGCEVPHKWSS-PEGGKKIDISNQIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYEGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVDIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKVPKDVDYARMYK 306

[Close]

BLAST of GR408666 vs. TrEMBL
Match: Q8RWN4_ARATH (Cytosolic IMP-GMP specific 5-nucleotidase, putative OS=Arabidopsis thaliana GN=At1g75210 PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 3.552e-76
Identity = 151/214 (70.56%), Postives = 170/214 (79.44%), Query Frame = 3

Query:  126 GTNQLHSQYSMDENHDAGIHDP--RSVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILER-SRGR*YARMYK 758
            GT+QL  + SM++   + + +   +   +   D    P  WSS PE G K DI  QIFCNRSLNMKNI+AVGFDMDYTLAQYK ETFESLAY GT++KLVYDLGYP+ELL W+F+W YMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKV  YG++LVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFK    E+  +   YARMYK
Sbjct:   94 GTSQLCFKSSMEDQKPSALSENVLQFHTVGSRDGCDVPHEWSS-PEGGKKIDISNQIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYDGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVEIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKVPKDVDYARMYK 306

[Close]

BLAST of GR408666 vs. TrEMBL
Match: Q9FRL2_ARATH (Cytosolic IMP-GMP specific 5'-nucleotidase, putative; 41097-37373 OS=Arabidopsis thaliana GN=F22H5.7 PE=4 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 5.130e-75
Identity = 145/186 (77.96%), Postives = 156/186 (83.87%), Query Frame = 3

Query:  204 IPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILER-SRGR*YARMYK 758
            +   D    P  WSS PE G K DI  QIFCNRSLNMKNI+AVGFDMDYTLAQYK ETFESLAY GT++KLVYDLGYP+ELL W+F+W YMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKV  YG++LVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFK    E+  +   YARMYK
Sbjct:   19 VGSRDGCDVPHEWSS-PEGGKKIDISNQIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYDGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVEIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKVPKDVDYARMYK 203

[Close]

BLAST of GR408666 vs. TrEMBL
Match: B8AKK2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13703 PE=4 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 3.804e-70
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 3

Query:  237 IWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            IWS+         +GK+IFCNRSLNM+NI AVGFDMDYTLAQYKPETFE+LAY GTI+KLV DLGYP ELL W F+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRE+SKE+KV  YG+TL+RDSFDEPDYALIDTLFSL EAYLFAQLVDF
Sbjct:   66 IWSTPSVESRTISVGKEIFCNRSLNMRNITAVGFDMDYTLAQYKPETFEALAYHGTIEKLVKDLGYPEELLTWQFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFREMSKEEKVSAYGSTLIRDSFDEPDYALIDTLFSLGEAYLFAQLVDF 221

[Close]

BLAST of GR408666 vs. TrEMBL
Match: Q7XZV8_ORYSJ (HAD superfamily hydrolase, 5'-Nucleotidase containing protein, expressed OS=Oryza sativa subsp. japonica GN=OSJNBb0094O03.19 PE=4 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 6.489e-70
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 3

Query:  237 IWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            IWS+         +GK+IFCNRSLNM+NI AVGFDMDYTLAQYKPETFE+LAY GTI+KLV DLGYP ELL W F+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRE+SKE+KV  YG+TL+RDSFDEPDYALIDTLFSL EAYLFAQLVDF
Sbjct:   66 IWSTPSVEPRTISVGKEIFCNRSLNMRNITAVGFDMDYTLAQYKPETFEALAYHGTIEKLVKDLGYPEELLTWQFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFREMSKEEKVSAYGSTLIRDSFDEPDYALIDTLFSLGEAYLFAQLVDF 221

[Close]

BLAST of GR408666 vs. TrEMBL
Match: B9F5Y2_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_12764 PE=3 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 6.489e-70
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 3

Query:  237 IWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            IWS+         +GK+IFCNRSLNM+NI AVGFDMDYTLAQYKPETFE+LAY GTI+KLV DLGYP ELL W F+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRE+SKE+KV  YG+TL+RDSFDEPDYALIDTLFSL EAYLFAQLVDF
Sbjct:   66 IWSTPSVEPRTISVGKEIFCNRSLNMRNITAVGFDMDYTLAQYKPETFEALAYHGTIEKLVKDLGYPEELLTWQFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFREMSKEEKVSAYGSTLIRDSFDEPDYALIDTLFSLGEAYLFAQLVDF 221

[Close]

BLAST of GR408666 vs. TrEMBL
Match: B7ECK3_ORYSJ (cDNA clone:J013088P07, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 6.489e-70
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 3

Query:  237 IWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            IWS+         +GK+IFCNRSLNM+NI AVGFDMDYTLAQYKPETFE+LAY GTI+KLV DLGYP ELL W F+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRE+SKE+KV  YG+TL+RDSFDEPDYALIDTLFSL EAYLFAQLVDF
Sbjct:   14 IWSTPSVEPRTISVGKEIFCNRSLNMRNITAVGFDMDYTLAQYKPETFEALAYHGTIEKLVKDLGYPEELLTWQFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFREMSKEEKVSAYGSTLIRDSFDEPDYALIDTLFSLGEAYLFAQLVDF 169

[Close]

BLAST of GR408666 vs. TrEMBL
Match: B6TW66_MAIZE (Cytosolic purine 5-nucleotidase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 1.888e-69
Identity = 126/156 (80.77%), Postives = 137/156 (87.82%), Query Frame = 3

Query:  237 IWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            +WSS         +GK++FCNRSLNM+NI AVGFDMDYTLAQYKPETFESLAY GTI+KLV DL YP ELL W F+WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGF+ELSKE+KV  YG+TL+RDSFDEPDYALIDTLFSL EAYLFAQLVDF
Sbjct:   67 VWSSPSAEPRSISVGKEVFCNRSLNMRNITAVGFDMDYTLAQYKPETFESLAYYGTIEKLVKDLRYPEELLTWEFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKEEKVAAYGSTLIRDSFDEPDYALIDTLFSLGEAYLFAQLVDF 222

[Close]

BLAST of GR408666 vs. TAIR peptide
Match: AT1G75210.1 (| Symbols: | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | chr1:28223990-28228751 FORWARD LENGTH=642)

HSP 1 Score: 288.886 bits (738), Expect = 2.271e-78
Identity = 151/214 (70.56%), Postives = 170/214 (79.44%), Query Frame = 3

Query:  126 GTNQLHSQYSMDENHDAGIHDP--RSVDIPGNDSGKQPRIWSSSPEHGFKTDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKIVILER-SRGR*YARMYK 758
            GT+QL  + SM++   + + +   +   +   D    P  WSS PE G K DI  QIFCNRSLNMKNI+AVGFDMDYTLAQYK ETFESLAY GT++KLVYDLGYP+ELL W+F+W YMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKV  YG++LVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFK    E+  +   YARMYK
Sbjct:   94 GTSQLCFKSSMEDQKPSALSENVLQFHTVGSRDGCDVPHEWSS-PEGGKKIDISNQIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYDGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVEIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKVPKDVDYARMYK 306

[Close]

BLAST of GR408666 vs. TAIR peptide
Match: AT5G48960.1 (| Symbols: | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | chr5:19849645-19853382 FORWARD LENGTH=642)

HSP 1 Score: 103.99 bits (258), Expect = 1.036e-22
Identity = 56/138 (40.58%), Postives = 91/138 (65.94%), Query Frame = 3

Query:  288 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVD 701
            IFC+R+LN+++I A+G+DMDYTL  Y    +E  AY   ++ L   +G+P + L  +F+ + ++RGL++DK++GN++K DR  YVK A HG + LS +     YG  LV D  ++  +  ++T FS++EA  +AQ+VD
Sbjct:  165 IFCSRTLNLRSISAIGYDMDYTLMHYNVMAWEGKAYDYCMENLK-SMGFPVDGL--AFDPELVIRGLMIDKEKGNLVKADRFGYVKRAMHGTKMLSNKAVSEIYGRELV-DLRNQSRWEFLNTFFSVSEALAYAQMVD 298

[Close]

BLAST of GR408666 vs. TAIR peptide
Match: AT2G23890.1 (| Symbols: | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | chr2:10170680-10173622 FORWARD LENGTH=553)

HSP 1 Score: 68.9366 bits (167), Expect = 3.693e-12
Identity = 44/141 (31.21%), Postives = 71/141 (50.35%), Query Frame = 3

Query:  288 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYQGTIKKLVYDLGYPSELLNWSFNWKYMVRGLVLDKKRGNILKMDRHKYVKV--AYHGFRELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDF 704
            I+ N++L + NI   GFD DYTLA Y     +SL Y    K +V +  YP     + ++  + +RGL  DK +G ++K+D    ++    Y G R+LS+++    YG   +          L+D  F  +EA L A +V +
Sbjct:   99 IYVNKNLRLDNIQVYGFDYDYTLAHYSSH-LQSLIYDLAKKHMVNEFRYPDVCTQFEYDPTFPIRGLYYDKLKGCLMKLDFFGSIEPDGCYFGRRKLSRKEIESMYGTRHIGRDQARGLVGLMD-FFCFSEACLIADMVQY 237

The following BLAST results are available for this feature:

BLAST of GR408666 vs. SwissProt
BLAST of GR408666 vs. TrEMBL
BLAST of GR408666 vs. TAIR peptide

BLAST of GR408666 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
5NTC_CHICK	1.742e-24	39.77	Cytosolic purine 5'-nucleotidase OS=Gallus gallus ...	[more]
5NTC_XENTR	2.275e-24	39.18	Cytosolic purine 5'-nucleotidase OS=Xenopus tropic...	[more]
5NTC_XENLA	1.129e-23	41.22	Cytosolic purine 5'-nucleotidase OS=Xenopus laevis...	[more]
5NTC_PONAB	1.129e-23	40.14	Cytosolic purine 5'-nucleotidase OS=Pongo abelii G...	[more]
5NTC_MOUSE	1.129e-23	40.14	Cytosolic purine 5'-nucleotidase OS=Mus musculus G...	[more]
5NTC_HUMAN	1.129e-23	40.14	Cytosolic purine 5'-nucleotidase OS=Homo sapiens G...	[more]
5NTC_BOVIN	1.129e-23	40.14	Cytosolic purine 5'-nucleotidase OS=Bos taurus GN=...	[more]
5NTC_DICDI	1.526e-20	38.82	Cytosolic purine 5'-nucleotidase OS=Dictyostelium ...	[more]
NT5D4_HUMAN	4.439e-20	39.57	5'-nucleotidase domain-containing protein 4 OS=Hom...	[more]
NT5D_DICDI	5.811e-12	31.29	5'-nucleotidase domain-containing protein DDB_G027...	[more]

BLAST of GR408666 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9IBG2_POPTR	2.013e-79	68.81	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9R7U4_RICCO	1.442e-77	81.52	Cytosolic purine 5-nucleotidase, putative OS=Ricin...	[more]
D7KSL6_ARALY	7.158e-77	63.14	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q8RWN4_ARATH	3.552e-76	70.56	Cytosolic IMP-GMP specific 5-nucleotidase, putativ...	[more]
Q9FRL2_ARATH	5.130e-75	77.96	Cytosolic IMP-GMP specific 5'-nucleotidase, putati...	[more]
B8AKK2_ORYSI	3.804e-70	81.41	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q7XZV8_ORYSJ	6.489e-70	81.41	HAD superfamily hydrolase, 5'-Nucleotidase contain...	[more]
B9F5Y2_ORYSJ	6.489e-70	81.41	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7ECK3_ORYSJ	6.489e-70	81.41	cDNA clone:J013088P07, full insert sequence OS=Ory...	[more]
B6TW66_MAIZE	1.888e-69	80.77	Cytosolic purine 5-nucleotidase OS=Zea mays PE=2 S...	[more]

BLAST of GR408666 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G75210.1	2.271e-78	70.56	\| Symbols: \| HAD-superfamily hydrolase, subfamily...	[more]
AT5G48960.1	1.036e-22	40.58	\| Symbols: \| HAD-superfamily hydrolase, subfamily...	[more]
AT2G23890.1	3.693e-12	31.21	\| Symbols: \| HAD-superfamily hydrolase, subfamily...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 2..21 score
None	No IPR available	PANTHER	PTHR12103:SF1	CYTOSOLIC PURINE 5-NUCLEOTIDASE	coord: 64..235 score: 6.2
None	No IPR available	SUPERFAMILY	56784	HAD-like	coord: 92..236 score: 2.8
IPR008380	HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase	PANTHER	PTHR12103	CYTOSOLIC PURINE 5-NUCLEOTIDASE-RELATED	coord: 64..235 score: 6.2
IPR008380	HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase	PFAM	PF05761	5_nucleotid	coord: 96..235 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408666 ID=GR408666; Name=GR408666; organism=Cicer arietinum; type=EST; length=812bp
GATTCTCATTTTCTCTCTCTAACTTCTCTCCTCAACTCGTAGCACCTCTT
TTATCTTCTCGCCTTTGCTTTGCCTTTGGAATTTCCGTTAGAACTTCTCG
CCCCTTCGCCAACATCGCATCACCAGGAACTAACCAGTTGCATTCTCAAT
ATTCAATGGACGAGAACCATGATGCTGGAATACATGACCCAAGATCAGTG
GATATACCTGGCAATGATAGTGGAAAACAGCCACGTATATGGTCATCTTC
TCCTGAACATGGATTTAAAACTGATATAGGCAAACAGATATTCTGTAACC
GGTCACTGAATATGAAGAACATTGTTGCTGTTGGATTTGACATGGATTAT
ACTCTGGCACAATACAAACCTGAAACTTTTGAATCCCTTGCTTATCAAGG
CACAATTAAAAAGCTGGTTTACGATTTGGGATATCCTAGCGAGTTACTAA
ATTGGTCTTTTAACTGGAAGTACATGGTCAGGGGCTTGGTTCTTGACAAA
AAAAGAGGAAATATCTTGAAGATGGATCGCCACAAGTATGTGAAAGTAGC
CTATCATGGATTTAGGGAGTTGTCAAAAGAAGATAAAGTTGGGACCTACG
GAAATACTTTGGTACGCGATTCATTTGACGAGCCAGACTATGCTCTAATT
GATACACTCTTTTCTCTTGCGGAAGCCTACTTGTTTGCTCAACTGGTTGA
TTTTAAGATTGTTATCCTGGAAAGATCGAGAGGGCGTTGATATGCTCGCA
TGTATAAGATGTCGAGCTGCAGTTGATTTGTGCACGTGATGGGACGTGAA
GCAATGGTTGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR008380	HAD-SF_hydro_IG_5-nucl

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