GR913311

EST Overview


Name	GR913311
Unique Name	GR913311
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	462

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR913311 vs. SwissProt
Match: NCASE_ARATH (Neutral ceramidase OS=Arabidopsis thaliana GN=At2g38010 PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.058e-44
Identity = 88/142 (61.97%), Postives = 103/142 (72.54%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGA-SKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            E+QF  AVELFN A+E+++GK+ ++HAY+DFS L+V V   G  S+ VKTC                    FKQGDDQGN FW+LVRN+L+TP  EQ+ CQ PKPILLDTGEMK PYDWAPSILPIQILRIGQ VILSVPGE
Sbjct:  378 EKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGE 519

[Close]

BLAST of GR913311 vs. SwissProt
Match: NCSEB_DICDI (Neutral ceramidase B OS=Dictyostelium discoideum GN=dcd2B PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.588e-24
Identity = 57/141 (40.43%), Postives = 76/141 (53.90%), Query Frame = 1

Query:   46 QFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQ--GNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            QFNKA+ELFN AS Q+ G V +RH+++ F+ + V            TC                    F Q D+   GNPFW  + + +  P  +QI CQ PKPILLD G M  P  W P ++PIQI+ IGQ V+++VPGE
Sbjct:  348 QFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGE 487

[Close]

BLAST of GR913311 vs. SwissProt
Match: NCSEA_DICDI (Neutral ceramidase A OS=Dictyostelium discoideum GN=dcd2A PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.715e-23
Identity = 52/140 (37.14%), Postives = 75/140 (53.57%), Query Frame = 1

Query:   46 QFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQ-GNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            QFNKA+ELFN A+ Q+ GK+ +RH +  F+ + V        +   TC                    F QGD+   NPFW  +  ++  P  +Q  CQ PKPIL+D G M  P  W P ++P+QI+ +GQ V+++VPGE
Sbjct:  343 QFNKALELFNNATIQVSGKIQYRHTWKPFTNVSVEAPYNSGVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTPQQTACQAPKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGE 481

[Close]

BLAST of GR913311 vs. SwissProt
Match: ASAH2_MOUSE (Neutral ceramidase OS=Mus musculus GN=Asah2 PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 6.905e-20
Identity = 47/139 (33.81%), Postives = 77/139 (55.40%), Query Frame = 1

Query:   49 FNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRN-LLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            + KA EL+  AS+++ G V   H +++ + + V +++T     VKTC                      QG  +G+PFW  +R+ LL  P +E ++CQ PKPILL +GE+ +P+ W P I+ +QI+ +G   I ++PGE
Sbjct:  381 YQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHT---VKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGE 516

[Close]

BLAST of GR913311 vs. SwissProt
Match: ASAH2_HUMAN (Neutral ceramidase OS=Homo sapiens GN=ASAH2 PE=1 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 1.178e-19
Identity = 48/139 (34.53%), Postives = 76/139 (54.68%), Query Frame = 1

Query:   49 FNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRN-LLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            + +A EL+  AS+++ G +   H ++D + + V ++ST ASK   TC                    F QG  +G+PFW  +R+ +L  P +E  +C  PKPILL TGE+  P+ W P I+ +QI+ +G   I ++PGE
Sbjct:  405 YQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASK---TCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGE 540

[Close]

BLAST of GR913311 vs. SwissProt
Match: ASA2C_HUMAN (Putative neutral ceramidase C OS=Homo sapiens GN=ASAH2C PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.178e-19
Identity = 48/139 (34.53%), Postives = 76/139 (54.68%), Query Frame = 1

Query:   49 FNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRN-LLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            + +A EL+  AS+++ G +   H ++D + + V ++ST ASK   TC                    F QG  +G+PFW  +R+ +L  P +E  +C  PKPILL TGE+  P+ W P I+ +QI+ +G   I ++PGE
Sbjct:  247 YQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASK---TCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGE 382

[Close]

BLAST of GR913311 vs. SwissProt
Match: ASAH2_RAT (Neutral ceramidase OS=Rattus norvegicus GN=Asah2 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 9.971e-19
Identity = 49/139 (35.25%), Postives = 74/139 (53.24%), Query Frame = 1

Query:   49 FNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRN-LLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            + KA EL   AS+++ G V   H +++ + + V +++T     VKTC                      QG  +GN FW  +R+ LL  P +E I+CQ PKPIL+ TGE+  P+ W P I+ IQI+ +G   I ++PGE
Sbjct:  386 YQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQLNATHT---VKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTGELTKPHPWQPDIVDIQIVTLGSLAIAAIPGE 521

[Close]

BLAST of GR913311 vs. SwissProt
Match: ASAH2_DANRE (Neutral ceramidase OS=Danio rerio GN=asah2 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.102e-17
Identity = 48/142 (33.80%), Postives = 70/142 (49.30%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRN-LLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            E  F  A EL+  A +++ G V   H +++ +   V  +ST   +   TC                    F QGD +G+PFW  +R+ +L  P  E   C  PKPIL  TGEM  P  W P+I+ +QI+ IG   +++VPGE
Sbjct:  360 ENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTHTGR---TCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKPILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGE 498

[Close]

BLAST of GR913311 vs. SwissProt
Match: NCASE_DROPS (Neutral ceramidase OS=Drosophila pseudoobscura pseudoobscura GN=CDase PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.079e-16
Identity = 47/148 (31.76%), Postives = 70/148 (47.30%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQ-----IKGKVDFRHAYIDFSQLEVNVSSTGASKL--VKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            +R  + A+ L N  S++     + G V F H ++D      +  +  + K+  ++ C                    F+QG    NP W  VR+ + TP QE I C  PKPILL TG    PY+W P I+  Q+L+IG  +I +VP E
Sbjct:  323 QRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSAYNPLSRKIDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIATPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCE 470

[Close]

BLAST of GR913311 vs. SwissProt
Match: NCASE_DROME (Neutral ceramidase OS=Drosophila melanogaster GN=CDase PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.032e-15
Identity = 46/148 (31.08%), Postives = 69/148 (46.62%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQ-----IKGKVDFRHAYIDFSQLEVNVSSTGASKL--VKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            +R  + A+ L N  S++     + G V F H ++D      +  +  + K+  ++ C                    F+QG    NP W  VR+ +  P QE I C  PKPILL TG    PY+W P I+  Q+L+IG  +I +VP E
Sbjct:  323 QRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCE 470

[Close]

BLAST of GR913311 vs. TrEMBL
Match: B9S377_RICCO (Ceramidase, putative OS=Ricinus communis GN=RCOM_0731110 PE=4 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.916e-48
Identity = 94/142 (66.20%), Postives = 108/142 (76.06%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGA-SKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF KAVELFN ASE++ GKVD+RH+YIDFSQLEV +   G  S+ VKTC                    FKQGDD+GN FW+LVRN LKTP++EQIDCQHPKPILLDTGEMK PYDWAPS+LP+QI+R+GQ VILSVPGE
Sbjct:  394 ERQFRKAVELFNKASEELNGKVDYRHSYIDFSQLEVTLPKEGGGSETVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNTFWRLVRNFLKTPNKEQIDCQHPKPILLDTGEMKQPYDWAPSVLPVQIVRVGQLVILSVPGE 535

[Close]

BLAST of GR913311 vs. TrEMBL
Match: A9YFM2_WHEAT (Neutral ceramidase OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 2.468e-47
Identity = 93/141 (65.96%), Postives = 110/141 (78.01%), Query Frame = 1

Query:   43 RQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVS-STGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            RQF KAV+LFN ASE+I+GK+D+RH Y+DFSQL+V+VS STG  ++VKTC                    FKQGDD+GNPFW+LVRNLLKTP +EQ++CQ PKPILLDTGEMK PYDWAP+ILPIQI+RIGQ VIL VPGE
Sbjct:  408 RQFLKAVDLFNSASEEIQGKIDYRHTYLDFSQLKVSVSTSTGGPQVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNPFWRLVRNLLKTPGKEQVECQAPKPILLDTGEMKEPYDWAPAILPIQIIRIGQLVILCVPGE 548

[Close]

BLAST of GR913311 vs. TrEMBL
Match: D7SK45_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024820001 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 3.224e-47
Identity = 92/142 (64.79%), Postives = 107/142 (75.35%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGA-SKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            +RQF KAV+LFN A+EQ+KGK+D+RH Y+DFS+L V +   G  S++VKTC                    FKQGDDQGNPFW+LVRN+LKTPD+ Q+DC HPKPILLDTGEM  PYDWAPSILPIQILRIGQ VILSVPGE
Sbjct:  495 DRQFRKAVDLFNKATEQLKGKIDYRHTYLDFSKLSVTLPKQGGGSEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNVLKTPDKVQMDCHHPKPILLDTGEMTKPYDWAPSILPIQILRIGQLVILSVPGE 636

[Close]

BLAST of GR913311 vs. TrEMBL
Match: B7ZZK1_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 4.655e-46
Identity = 90/141 (63.83%), Postives = 109/141 (77.30%), Query Frame = 1

Query:   43 RQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNV-SSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            RQF KAV+LF+ ASE+I+GK+D+RH Y+DFS+LEVNV +STG+ ++VKTC                    FKQGD +GNPFW+LVRNLLKTP +EQ++CQ PKPILLDTGEMK PYDWAP+ILPIQI+RIGQ  IL VPGE
Sbjct:  408 RQFQKAVDLFSSASEEIQGKIDYRHTYLDFSKLEVNVRTSTGSQQVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDVKGNPFWRLVRNLLKTPGKEQVECQAPKPILLDTGEMKEPYDWAPAILPIQIIRIGQLAILCVPGE 548

[Close]

BLAST of GR913311 vs. TrEMBL
Match: D7MR59_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_685566 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 6.080e-46
Identity = 92/142 (64.79%), Postives = 104/142 (73.24%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSS-TGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF KA +LF  ASE+I+GKVD+RHA++DFSQLEV ++   G SK+VKTC                    FKQGDDQGNPFW+LVRNLLK P +EQ  CQ PKPILLDTGEMK PYDWAPSILP+QIL IGQ VIL VPGE
Sbjct:  355 ERQFKKAADLFTKASEEIQGKVDYRHAFVDFSQLEVTINGQNGGSKVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQARCQRPKPILLDTGEMKQPYDWAPSILPVQILCIGQLVILCVPGE 496

[Close]

BLAST of GR913311 vs. TrEMBL
Match: C5XEC0_SORBI (Putative uncharacterized protein Sb03g028410 OS=Sorghum bicolor GN=Sb03g028410 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 2.310e-45
Identity = 92/142 (64.79%), Postives = 106/142 (74.65%), Query Frame = 1

Query:   43 RQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSST--GASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            RQF KAV+LFN ASE+I+GKVD+RH Y+DFSQLEVNVSS+  G  + VKTC                    FKQGD +GNPFW+LVRN+L+ P  EQ+ CQ PKPILLDTGEMK+PYDWAPSILPIQI+RIGQ VIL VPGE
Sbjct:  336 RQFLKAVDLFNSASEEIQGKVDYRHTYLDFSQLEVNVSSSTGGQHQTVKTCPAAMGFAFAAGTTDGPGAFDFKQGDVKGNPFWRLVRNVLRPPGPEQVKCQAPKPILLDTGEMKVPYDWAPSILPIQIIRIGQLVILCVPGE 477

[Close]

BLAST of GR913311 vs. TrEMBL
Match: B5TWK7_HORVU (Neutral ceramidase OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 3.941e-45
Identity = 89/141 (63.12%), Postives = 108/141 (76.60%), Query Frame = 1

Query:   43 RQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVS-STGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            RQF KAV+LFN ASE+I+GK+D+RH Y+DFSQL+V+VS STG  ++VKTC                    FKQGDD+GNPFW+LV  +LK P ++Q++CQ PKPILLDTGEMK PYDWAP+ILPIQI+RIGQ VILSVPGE
Sbjct:  408 RQFLKAVDLFNSASEEIQGKIDYRHTYLDFSQLKVSVSTSTGGPQVVKTCPAAMGFSFAAGTTDGPGAFDFKQGDDKGNPFWRLVGGILKKPGKQQVECQAPKPILLDTGEMKEPYDWAPAILPIQIIRIGQLVILSVPGE 548

[Close]

BLAST of GR913311 vs. TrEMBL
Match: D7KH17_ARALY (Ceramidase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_311800 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.498e-44
Identity = 89/142 (62.68%), Postives = 104/142 (73.24%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNV-SSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF  A+ELFN ASEQ++GKVD+RH Y+DFSQL V +    G S++VKTC                    F QGDD+GNPFW+LVRN+LKTPD++QIDC +PKPILLDTGEM  PYDWAPSIL +QILRIGQ  ILSVPGE
Sbjct:  395 ERQFKMALELFNKASEQLEGKVDYRHVYVDFSQLNVTLPEKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQILRIGQLFILSVPGE 536

[Close]

BLAST of GR913311 vs. TrEMBL
Match: Q9LNV7_ARATH (F22G5.28 OS=Arabidopsis thaliana GN=At1g07380 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.956e-44
Identity = 88/142 (61.97%), Postives = 104/142 (73.24%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNV-SSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF  A+ELFN ASEQ++GKVD+RH Y+DFSQL V +    G S++VKTC                    F QGDD+GNPFW+LVRN+LKTPD++QIDC +PKPILLDTGEM  PYDWAPSIL +Q+LRIGQ  ILSVPGE
Sbjct:  399 ERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGE 540

[Close]

BLAST of GR913311 vs. TrEMBL
Match: B9SPF3_RICCO (Ceramidase, putative OS=Ricinus communis GN=RCOM_0085480 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.336e-44
Identity = 90/141 (63.83%), Postives = 104/141 (73.76%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            E+QF KAV+LFNGA+EQ+KGKV + HAYIDFS LEV++ +    K++KTC                    FKQGDD+GN FWKLVRN+LKTP  EQI CQ PKPILLDTGEMK PYDWAPSILP+QIL+IGQ VILSVP E
Sbjct:  406 EKQFRKAVDLFNGATEQLKGKVQYSHAYIDFSNLEVSLGN----KVIKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKTPGPEQIKCQLPKPILLDTGEMKEPYDWAPSILPVQILQIGQLVILSVPSE 542

[Close]

BLAST of GR913311 vs. TAIR peptide
Match: AT5G58980.1 (| Symbols: | Neutral/alkaline non-lysosomal ceramidase | chr5:23811449-23814395 REVERSE LENGTH=733)

HSP 1 Score: 191.43 bits (485), Expect = 1.829e-49
Identity = 93/142 (65.49%), Postives = 106/142 (74.65%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSS-TGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF KA +LF  ASE+I+GKVD+RHAY+DFSQLEV ++   G S++VKTC                    FKQGDDQGNPFW+LVRNLLK P +EQ+ CQ PKPILLDTGEMK PYDWAPSILP+QILRIGQ VIL VPGE
Sbjct:  355 ERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGE 496

[Close]

BLAST of GR913311 vs. TAIR peptide
Match: AT1G07380.1 (| Symbols: | Neutral/alkaline non-lysosomal ceramidase | chr1:2264829-2268306 REVERSE LENGTH=779)

HSP 1 Score: 181.8 bits (460), Expect = 1.449e-46
Identity = 88/142 (61.97%), Postives = 104/142 (73.24%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNV-SSTGASKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            ERQF  A+ELFN ASEQ++GKVD+RH Y+DFSQL V +    G S++VKTC                    F QGDD+GNPFW+LVRN+LKTPD++QIDC +PKPILLDTGEM  PYDWAPSIL +Q+LRIGQ  ILSVPGE
Sbjct:  398 ERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGE 539

[Close]

BLAST of GR913311 vs. TAIR peptide
Match: AT2G38010.2 (| Symbols: | Neutral/alkaline non-lysosomal ceramidase | chr2:15906862-15909867 FORWARD LENGTH=792)

HSP 1 Score: 178.718 bits (452), Expect = 1.227e-45
Identity = 88/142 (61.97%), Postives = 103/142 (72.54%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGA-SKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            E+QF  AVELFN A+E+++GK+ ++HAY+DFS L+V V   G  S+ VKTC                    FKQGDDQGN FW+LVRN+L+TP  EQ+ CQ PKPILLDTGEMK PYDWAPSILPIQILRIGQ VILSVPGE
Sbjct:  413 EKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGE 554

[Close]

BLAST of GR913311 vs. TAIR peptide
Match: AT2G38010.1 (| Symbols: | Neutral/alkaline non-lysosomal ceramidase | chr2:15906862-15909867 FORWARD LENGTH=757)

HSP 1 Score: 178.718 bits (452), Expect = 1.227e-45
Identity = 88/142 (61.97%), Postives = 103/142 (72.54%), Query Frame = 1

Query:   40 ERQFNKAVELFNGASEQIKGKVDFRHAYIDFSQLEVNVSSTGA-SKLVKTCXXXXXXXXXXXXXXXXXXXXFKQGDDQGNPFWKLVRNLLKTPDQEQIDCQHPKPILLDTGEMKLPYDWAPSILPIQILRIGQFVILSVPGE 462
            E+QF  AVELFN A+E+++GK+ ++HAY+DFS L+V V   G  S+ VKTC                    FKQGDDQGN FW+LVRN+L+TP  EQ+ CQ PKPILLDTGEMK PYDWAPSILPIQILRIGQ VILSVPGE
Sbjct:  378 EKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGE 519

The following BLAST results are available for this feature:

BLAST of GR913311 vs. SwissProt
BLAST of GR913311 vs. TrEMBL
BLAST of GR913311 vs. TAIR peptide

BLAST of GR913311 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NCASE_ARATH	1.058e-44	61.97	Neutral ceramidase OS=Arabidopsis thaliana GN=At2g...	[more]
NCSEB_DICDI	1.588e-24	40.43	Neutral ceramidase B OS=Dictyostelium discoideum G...	[more]
NCSEA_DICDI	8.715e-23	37.14	Neutral ceramidase A OS=Dictyostelium discoideum G...	[more]
ASAH2_MOUSE	6.905e-20	33.81	Neutral ceramidase OS=Mus musculus GN=Asah2 PE=1 S...	[more]
ASAH2_HUMAN	1.178e-19	34.53	Neutral ceramidase OS=Homo sapiens GN=ASAH2 PE=1 S...	[more]
ASA2C_HUMAN	1.178e-19	34.53	Putative neutral ceramidase C OS=Homo sapiens GN=A...	[more]
ASAH2_RAT	9.971e-19	35.25	Neutral ceramidase OS=Rattus norvegicus GN=Asah2 P...	[more]
ASAH2_DANRE	1.102e-17	33.80	Neutral ceramidase OS=Danio rerio GN=asah2 PE=1 SV...	[more]
NCASE_DROPS	2.079e-16	31.76	Neutral ceramidase OS=Drosophila pseudoobscura pse...	[more]
NCASE_DROME	1.032e-15	31.08	Neutral ceramidase OS=Drosophila melanogaster GN=C...	[more]

BLAST of GR913311 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S377_RICCO	2.916e-48	66.20	Ceramidase, putative OS=Ricinus communis GN=RCOM_0...	[more]
A9YFM2_WHEAT	2.468e-47	65.96	Neutral ceramidase OS=Triticum aestivum PE=2 SV=1	[more]
D7SK45_VITVI	3.224e-47	64.79	Whole genome shotgun sequence of line PN40024, sca...	[more]
B7ZZK1_MAIZE	4.655e-46	63.83	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
D7MR59_ARALY	6.080e-46	64.79	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
C5XEC0_SORBI	2.310e-45	64.79	Putative uncharacterized protein Sb03g028410 OS=So...	[more]
B5TWK7_HORVU	3.941e-45	63.12	Neutral ceramidase OS=Hordeum vulgare PE=2 SV=1	[more]
D7KH17_ARALY	1.498e-44	62.68	Ceramidase family protein OS=Arabidopsis lyrata su...	[more]
Q9LNV7_ARATH	1.956e-44	61.97	F22G5.28 OS=Arabidopsis thaliana GN=At1g07380 PE=4...	[more]
B9SPF3_RICCO	3.336e-44	63.83	Ceramidase, putative OS=Ricinus communis GN=RCOM_0...	[more]

BLAST of GR913311 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G58980.1	1.829e-49	65.49	\| Symbols: \| Neutral/alkaline non-lysosomal ceram...	[more]
AT1G07380.1	1.449e-46	61.97	\| Symbols: \| Neutral/alkaline non-lysosomal ceram...	[more]
AT2G38010.2	1.227e-45	61.97	\| Symbols: \| Neutral/alkaline non-lysosomal ceram...	[more]
AT2G38010.1	1.227e-45	61.97	\| Symbols: \| Neutral/alkaline non-lysosomal ceram...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006823	Neutral/alkaline nonlysosomal ceramidase	PANTHER	PTHR12670	CERAMIDASE	coord: 7..152 score: 6.1
None	No IPR available	PFAM	PF04734	Ceramidase_alk	coord: 7..152 score: 5
None	No IPR available	SEG	seg	seg	coord: 1..11 score: NAcoord: 62..81 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR913311 ID=GR913311; Name=GR913311; organism=Cicer arietinum; type=EST; length=462bp
GATTAGCGTGGTCGCGGCCGAGGTACACGTATTATTGGGGAGAGACAGTT
TAATAAAGCAGTGGAGCTATTTAATGGAGCATCCGAACAGATTAAAGGAA
AGGTAGATTTTCGACATGCCTACATCGATTTCTCCCAGCTCGAGGTAAAT
GTTTCTAGCACAGGAGCTTCTAAGCTAGTAAAGACATGTCCAGCTGCAAT
GGGATTTGGATTTGCCGCTGGAACAACTGATGGACCTGGAGCATTTGATT
TTAAGCAAGGCGATGATCAGGGGAATCCATTCTGGAAACTGGTCCGCAAC
TTACTGAAAACGCCAGACCAGGAACAGATAGATTGTCAACACCCAAAGCC
TATTTTGCTTGATACCGGTGAAATGAAGCTTCCATATGATTGGGCACCTT
CAATACTTCCAATCCAGATCCTCCGAATTGGGCAGTTTGTTATTCTTAGT
GTACCAGGAGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR006823	Ceramidase_alk

Search form