GR915146

EST Overview


Name	GR915146
Unique Name	GR915146
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	424

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYC_SOYBN (Pyruvate kinase, cytosolic isozyme OS=Glycine max PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 4.073e-28
Identity = 64/138 (46.38%), Postives = 92/138 (66.67%), Query Frame = 1

Query:   22 AGTIK----NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            AGT++    N+ATL G    ++   V ++LPTLTEKDKE I  WGV NKID ++LS+ R   D+   R+ L         Q+ +K+EN EG+ +FDEIL+E D  +++RG+LG+++P EK+FL QK  +YKCN+ GKP
Sbjct:  160 AGTVRCRCENTATL-GERKNVNLPGVVVDLPTLTEKDKEDILGWGVPNKIDMIALSFVRKGSDLVNVRKVLGP--HAKNIQLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKP 294

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYC_SOLTU (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 4.503e-27
Identity = 62/137 (45.26%), Postives = 92/137 (67.15%), Query Frame = 1

Query:   22 AGTIK----NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGK 420
            +GT++    N+ATL G    ++   V ++LPTLTEKDKE I  WGV N ID ++LS+ R   D+   R+ L       + Q+ +K+EN EG+ +FDEIL+E D  +++RG+LG+++P EK+FL QK  +YKCN+AGK
Sbjct:  159 SGTVRCRCENTATL-GERKNVNLPGVVVDLPTLTEKDKEDILEWGVPNNIDMIALSFVRKGSDLVNVRKVLGP--HAKRIQLMSKVENQEGVINFDEILRETDSFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGK 292

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_EIMTE (Pyruvate kinase OS=Eimeria tenella GN=PYK PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.310e-26
Identity = 59/125 (47.20%), Postives = 86/125 (68.80%), Query Frame = 1

Query:   49 LAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            + G+   ++   V+++LP + EKDK  I  +G+    +F++ S+ + A+DVR  R  L   G     +I  KIENVEGL +FDEILQEADGI+++RG+LG+++PPEKVFL QK  + KCN+AGKP
Sbjct:  206 IIGNKKNMNLPGVKVDLPVIGEKDKNDILNFGIPMGCNFIAASFVQSADDVRYIRSILGTKG--RNIKIIPKIENVEGLLNFDEILQEADGIMIARGDLGMEIPPEKVFLAQKMMISKCNVAGKP 328

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYC_ARATH (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana GN=At4g26390 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 5.505e-25
Identity = 55/136 (40.44%), Postives = 88/136 (64.71%), Query Frame = 1

Query:   25 GTIK---NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            GT++    + ++ G    ++   V ++LPTLTEKDK+ I  WGV N+ID ++LS+ R   D+ + R+ L K        + +K+EN EG+ +FD+IL  +D  +++RG+LG+++P EK+FL QK  +YKCN  GKP
Sbjct:  147 GTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGK--HAKTILLMSKVENQEGVANFDDILINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKP 280

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYC_TOBAC (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 1.602e-24
Identity = 55/130 (42.31%), Postives = 86/130 (66.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+A L G    ++   V ++LPTLT+KDK+ I  WGV N ID ++LS+ R   D+ E R+ L +        + +K+EN EG+ +FD+IL  +D  +++RG+LG+++P EK+FL QK  +YKCN+ GKP
Sbjct:  165 ENTAVL-GERKNVNLPGVIVDLPTLTDKDKDDILNWGVPNHIDMIALSFVRKGSDLVEVRKLLGE--HAKNILLMSKVENQEGVANFDDILLNSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKP 291

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_EMENI (Pyruvate kinase OS=Emericella nidulans GN=pkiA PE=3 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 1.038e-23
Identity = 53/111 (47.75%), Postives = 78/111 (70.27%), Query Frame = 1

Query:   91 IELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            ++LP L+EKD   +  +GVKNK+D +  S+ R   D+R  RE L + G   + QI AKIEN +G+ +FDEIL+E DG++++RG+LGI++P  KVF+ QK  + KCN+ GKP
Sbjct:  200 VDLPALSEKDISDLK-FGVKNKVDMVFASFIRRGSDIRHIREVLGEEG--REIQIIAKIENQQGVNNFDEILEETDGVMVARGDLGIEIPAPKVFIAQKMMIAKCNIKGKP 307

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_AGABI (Pyruvate kinase OS=Agaricus bisporus GN=pkiA PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.356e-23
Identity = 56/129 (43.41%), Postives = 89/129 (68.99%), Query Frame = 1

Query:   37 NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            N+ TL+ S   ++  +  ++LP L+EKDK+ +  +G++N +D +  S+ R  EDVR+ RE L   G  +  +I  KIEN +G+ +FDEIL+EADG++++RG+LGI++P  +VFL QK  + KCN+ GKP
Sbjct:  183 NNGTLS-SRKGVNLPKTDVDLPPLSEKDKDDLR-FGIRNGVDMIFASFIRRGEDVRQIREVLGPDG--ASIKIIVKIENEQGVANFDEILKEADGVMVARGDLGIEIPASQVFLAQKMMIAKCNIVGKP 307

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_BACST (Pyruvate kinase OS=Bacillus stearothermophilus GN=pyk PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 2.313e-23
Identity = 55/113 (48.67%), Postives = 79/113 (69.91%), Query Frame = 1

Query:   85 VRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            V++ LP +TEKD+  I  +G++  IDF++ S+ R A DV E RE L +  D    QI AKIEN EG+ + DEIL+ ADG++++RG+LG+++P E+V L QK  + KCNM GKP
Sbjct:  164 VKVNLPGITEKDRADI-LFGIRQGIDFIAASFVRRASDVLEIRELL-EAHDALHIQIIAKIENEEGVANIDEILEAADGLMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKP 274

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_ASPNG (Pyruvate kinase OS=Aspergillus niger GN=pkiA PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 2.313e-23
Identity = 52/111 (46.85%), Postives = 78/111 (70.27%), Query Frame = 1

Query:   91 IELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            ++LP L+EKD   +  +GV+NK+D +  S+ R   D+R  RE L + G   + QI AKIEN +G+ +FDEIL+E DG++++RG+LGI++P  KVF+ QK  + KCN+ GKP
Sbjct:  200 VDLPALSEKDIADLK-FGVRNKVDMVFASFIRRGSDIRHIREVLGEEG--KEIQIIAKIENQQGVNNFDEILEETDGVMVARGDLGIEIPAPKVFIAQKMMIAKCNIKGKP 307

[Close]

BLAST of GR915146 vs. SwissProt
Match: KPYK_NEUCR (Pyruvate kinase OS=Neurospora crassa GN=pyk-1 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 5.152e-23
Identity = 53/111 (47.75%), Postives = 78/111 (70.27%), Query Frame = 1

Query:   91 IELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            ++LP L+EKDK  +  +GVKNK+D +  S+ R  +D+++ RE L + G   Q QI AKIEN +GL +F EIL E DG++++RG+LGI++P  +VF  QK  +  CN+AGKP
Sbjct:  199 VDLPALSEKDKADLR-FGVKNKVDMVFASFIRRGQDIKDIREVLGEDG--KQIQIIAKIENRQGLNNFAEILAETDGVMVARGDLGIEIPAAEVFAAQKKIIAMCNIAGKP 306

[Close]

BLAST of GR915146 vs. TrEMBL
Match: Q1SN32_MEDTR (Pyruvate kinase OS=Medicago truncatula GN=MtrDRAFT_AC139525g8v2 PE=3 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 7.631e-65
Identity = 124/131 (94.66%), Postives = 129/131 (98.47%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATL GSLFTLHASQ+ I+LPTLTEKDKEVISTWGVKNKIDFLSLSYTRHA+DVREAR+FLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSAL+KCNMAGKP
Sbjct:  179 IKNSATLTGSLFTLHASQIHIDLPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAKDVREARDFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALHKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TrEMBL
Match: B7FM31_MEDTR (Pyruvate kinase (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 7.631e-65
Identity = 124/131 (94.66%), Postives = 129/131 (98.47%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATL GSLFTLHASQ+ I+LPTLTEKDKEVISTWGVKNKIDFLSLSYTRHA+DVREAR+FLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSAL+KCNMAGKP
Sbjct:  163 IKNSATLTGSLFTLHASQIHIDLPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAKDVREARDFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALHKCNMAGKP 293

[Close]

BLAST of GR915146 vs. TrEMBL
Match: A9P7U5_POPTR (Pyruvate kinase OS=Populus trichocarpa GN=POPTRDRAFT_589553 PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 2.900e-64
Identity = 122/131 (93.13%), Postives = 129/131 (98.47%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATLAGSLFTLHASQ+RIELPTL++KDKEVISTWGV+NKIDFLSLSYTRHAEDVR ARE+LSKLGDL QTQIFAKIEN+EGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  179 IKNSATLAGSLFTLHASQIRIELPTLSDKDKEVISTWGVQNKIDFLSLSYTRHAEDVRNAREYLSKLGDLYQTQIFAKIENIEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TrEMBL
Match: D7UBT5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_74.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034684001 PE=4 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 2.455e-63
Identity = 119/131 (90.84%), Postives = 128/131 (97.71%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATLAGSL+TLH SQ+RI+LPTLT+KDKEVISTWGV+NKIDFLSLSYTRHAEDVR AREFLSKLGDL+QTQIFAKIEN+EGL HFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+A+YKCNMAGKP
Sbjct:  166 IKNSATLAGSLYTLHVSQIRIDLPTLTDKDKEVISTWGVQNKIDFLSLSYTRHAEDVRHAREFLSKLGDLNQTQIFAKIENIEGLNHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKP 296

[Close]

BLAST of GR915146 vs. TrEMBL
Match: A5BDS1_VITVI (Pyruvate kinase OS=Vitis vinifera GN=VITISV_026975 PE=3 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 2.455e-63
Identity = 119/131 (90.84%), Postives = 128/131 (97.71%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATLAGSL+TLH SQ+RI+LPTLT+KDKEVISTWGV+NKIDFLSLSYTRHAEDVR AREFLSKLGDL+QTQIFAKIEN+EGL HFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+A+YKCNMAGKP
Sbjct:  166 IKNSATLAGSLYTLHVSQIRIDLPTLTDKDKEVISTWGVQNKIDFLSLSYTRHAEDVRHAREFLSKLGDLNQTQIFAKIENIEGLNHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKP 296

[Close]

BLAST of GR915146 vs. TrEMBL
Match: Q9LF93_ARATH (Pyruvate kinase OS=Arabidopsis thaliana GN=F8J2_160 PE=3 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.897e-62
Identity = 118/130 (90.77%), Postives = 125/130 (96.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLH+SQV I+LPTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  167 RNAATLAGSLFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 296

[Close]

BLAST of GR915146 vs. TrEMBL
Match: Q94KE3_ARATH (Pyruvate kinase OS=Arabidopsis thaliana GN=At3g52990 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.897e-62
Identity = 118/130 (90.77%), Postives = 125/130 (96.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLH+SQV I+LPTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  180 RNAATLAGSLFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TrEMBL
Match: D7TIZ5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033747001 PE=4 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.897e-62
Identity = 118/131 (90.08%), Postives = 125/131 (95.42%), Query Frame = 1

Query:   31 IKNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            IKNSATLAGSLFTLH SQ+ I+LPTL++KDKEVISTWGVKNKIDFLSLSYTRHAEDVR AR+ LSKLGDL QTQIFAKIENVEGL HFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+A+YKCNMAGKP
Sbjct:  166 IKNSATLAGSLFTLHVSQIHIDLPTLSDKDKEVISTWGVKNKIDFLSLSYTRHAEDVRHARDHLSKLGDLHQTQIFAKIENVEGLNHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKP 296

[Close]

BLAST of GR915146 vs. TrEMBL
Match: A8MR07_ARATH (Pyruvate kinase OS=Arabidopsis thaliana GN=AT3G52990 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.897e-62
Identity = 118/130 (90.77%), Postives = 125/130 (96.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLH+SQV I+LPTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  127 RNAATLAGSLFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 256

[Close]

BLAST of GR915146 vs. TrEMBL
Match: D7LUD2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_906680 PE=4 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 1.759e-61
Identity = 118/130 (90.77%), Postives = 124/130 (95.38%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLHASQV I+LPTL EKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  180 RNAATLAGSLFTLHASQVHIDLPTLPEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT3G52990.2 (| Symbols: | Pyruvate kinase family protein | chr3:19649336-19652237 FORWARD LENGTH=474)

HSP 1 Score: 239.58 bits (610), Expect = 4.848e-64
Identity = 118/130 (90.77%), Postives = 125/130 (96.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLH+SQV I+LPTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  127 RNAATLAGSLFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 256

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT3G52990.1 (| Symbols: | Pyruvate kinase family protein | chr3:19649046-19652237 FORWARD LENGTH=527)

HSP 1 Score: 239.58 bits (610), Expect = 4.848e-64
Identity = 118/130 (90.77%), Postives = 125/130 (96.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATLAGSLFTLH+SQV I+LPTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ RE L KLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  180 RNAATLAGSLFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT2G36580.1 (| Symbols: | Pyruvate kinase family protein | chr2:15339253-15342781 FORWARD LENGTH=527)

HSP 1 Score: 230.72 bits (587), Expect = 2.252e-61
Identity = 113/130 (86.92%), Postives = 121/130 (93.08%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +N+ATL G LFTLH SQV I++PTLTEKDKEVISTWGV+NKIDFLSLSY RHAEDVR+ARE L+  GDLSQTQIFAKIEN EGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQK+ALYKCNMAGKP
Sbjct:  180 RNAATLGGPLFTLHVSQVHIDMPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQARELLNSCGDLSQTQIFAKIENEEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKP 309

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT5G56350.1 (| Symbols: | Pyruvate kinase family protein | chr5:22820254-22822529 REVERSE LENGTH=498)

HSP 1 Score: 115.931 bits (289), Expect = 8.084e-27
Identity = 58/130 (44.62%), Postives = 87/130 (66.92%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +NSA L G    ++   V ++LPTLTEKDKE I  WGV N+ID ++LS+ R   D+ + R+ L K        + +K+EN EG+ +FD+IL  +D  +++RG+LG+++P EK+FL QK  +YKCN+ GKP
Sbjct:  155 ENSAML-GERKNVNLPGVVVDLPTLTEKDKEDIMQWGVPNQIDMIALSFVRKGSDLVQVRKLLGK--HAKNILLMSKVENQEGVANFDDILVNSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKP 281

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT5G63680.1 (| Symbols: | Pyruvate kinase family protein | chr5:25490507-25492530 FORWARD LENGTH=510)

HSP 1 Score: 114.39 bits (285), Expect = 2.352e-26
Identity = 60/138 (43.48%), Postives = 89/138 (64.49%), Query Frame = 1

Query:   22 AGTI----KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            AGT+    +N+A L G    ++   V ++LPTLT+KD E I  WGV N ID ++LS+ R   D+   R+ L          + +K+EN EG+ +FDEIL+E D  +++RG+LG+++P EK+FL QK  +YKCN+AGKP
Sbjct:  159 AGTVICRCENTAML-GERKNVNLPGVVVDLPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS--KSIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKP 293

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT5G08570.1 (| Symbols: | Pyruvate kinase family protein | chr5:2778433-2780300 FORWARD LENGTH=510)

HSP 1 Score: 114.005 bits (284), Expect = 3.072e-26
Identity = 60/138 (43.48%), Postives = 89/138 (64.49%), Query Frame = 1

Query:   22 AGTIK----NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +GT++    NSA L G    ++   V ++LPTLT+KD E I  WGV N ID ++LS+ R   D+   R+ L          + +K+EN EG+ +FDEIL+E D  +++RG+LG+++P EK+FL QK  +YKCN+AGKP
Sbjct:  159 SGTVRCRCENSAML-GERKNVNLPGVVVDLPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLGS--HAKSIMLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKP 293

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT4G26390.1 (| Symbols: | Pyruvate kinase family protein | chr4:13342207-13344418 FORWARD LENGTH=497)

HSP 1 Score: 112.849 bits (281), Expect = 6.844e-26
Identity = 55/136 (40.44%), Postives = 88/136 (64.71%), Query Frame = 1

Query:   25 GTIK---NSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            GT++    + ++ G    ++   V ++LPTLTEKDK+ I  WGV N+ID ++LS+ R   D+ + R+ L K        + +K+EN EG+ +FD+IL  +D  +++RG+LG+++P EK+FL QK  +YKCN  GKP
Sbjct:  147 GTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGK--HAKTILLMSKVENQEGVANFDDILINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKP 280

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT3G25960.1 (| Symbols: | Pyruvate kinase family protein | chr3:9498439-9499932 FORWARD LENGTH=497)

HSP 1 Score: 108.997 bits (271), Expect = 9.883e-25
Identity = 57/131 (43.51%), Postives = 90/131 (68.70%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQT-QIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +NSA L G    ++   + ++LPTLTEKDKE I  WGV NKID ++LS+ R   D+ + R+ L   G+ S++  + +K+EN EG+ +FD+IL+ +D  +++RG+LG+++P EK+FL QK+ + K N  GKP
Sbjct:  163 ENSALL-GERKNVNLPGIVVDLPTLTEKDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLL---GEHSKSIMLMSKVENQEGVMNFDKILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKP 289

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT3G04050.1 (| Symbols: | Pyruvate kinase family protein | chr3:1049795-1051522 FORWARD LENGTH=510)

HSP 1 Score: 108.612 bits (270), Expect = 1.291e-24
Identity = 55/130 (42.31%), Postives = 86/130 (66.15%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +NSA L G    ++   + ++LPTLTEKD+E I  WGV NKID ++LS+ R   D+ E R+ L +  +     + +K+EN EG+ +FD+IL+ +D  +++RG+LG+++P EK+FL QK  + K N  GKP
Sbjct:  163 ENSAVL-GERKNVNLPGIVVDLPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLGE--NAKSIMLMSKVENQEGVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKP 289

[Close]

BLAST of GR915146 vs. TAIR peptide
Match: AT3G55650.1 (| Symbols: | Pyruvate kinase family protein | chr3:20647085-20648617 FORWARD LENGTH=510)

HSP 1 Score: 103.219 bits (256), Expect = 5.423e-23
Identity = 53/130 (40.77%), Postives = 83/130 (63.85%), Query Frame = 1

Query:   34 KNSATLAGSLFTLHASQVRIELPTLTEKDKEVISTWGVKNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKSALYKCNMAGKP 423
            +NSA L G    ++   + ++LPTLTEKDKE I  WGV NKID ++LS+ R   D+ E R  L +        + +K+EN EG+ + ++IL+ +D  +++RG+LG+++P EK+FL QK+ +   N  GKP
Sbjct:  163 ENSAIL-GERKNVNLPGIVVDLPTLTEKDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGE--HSKNIMLMSKVENQEGVMNCEKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKP 289

The following BLAST results are available for this feature:

BLAST of GR915146 vs. SwissProt
BLAST of GR915146 vs. TrEMBL
BLAST of GR915146 vs. TAIR peptide

BLAST of GR915146 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
KPYC_SOYBN	4.073e-28	46.38	Pyruvate kinase, cytosolic isozyme OS=Glycine max ...	[more]
KPYC_SOLTU	4.503e-27	45.26	Pyruvate kinase, cytosolic isozyme OS=Solanum tube...	[more]
KPYK_EIMTE	1.310e-26	47.20	Pyruvate kinase OS=Eimeria tenella GN=PYK PE=2 SV=...	[more]
KPYC_ARATH	5.505e-25	40.44	Probable pyruvate kinase, cytosolic isozyme OS=Ara...	[more]
KPYC_TOBAC	1.602e-24	42.31	Pyruvate kinase, cytosolic isozyme OS=Nicotiana ta...	[more]
KPYK_EMENI	1.038e-23	47.75	Pyruvate kinase OS=Emericella nidulans GN=pkiA PE=...	[more]
KPYK_AGABI	1.356e-23	43.41	Pyruvate kinase OS=Agaricus bisporus GN=pkiA PE=2 ...	[more]
KPYK_BACST	2.313e-23	48.67	Pyruvate kinase OS=Bacillus stearothermophilus GN=...	[more]
KPYK_ASPNG	2.313e-23	46.85	Pyruvate kinase OS=Aspergillus niger GN=pkiA PE=3 ...	[more]
KPYK_NEUCR	5.152e-23	47.75	Pyruvate kinase OS=Neurospora crassa GN=pyk-1 PE=3...	[more]

BLAST of GR915146 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q1SN32_MEDTR	7.631e-65	94.66	Pyruvate kinase OS=Medicago truncatula GN=MtrDRAFT...	[more]
B7FM31_MEDTR	7.631e-65	94.66	Pyruvate kinase (Fragment) OS=Medicago truncatula ...	[more]
A9P7U5_POPTR	2.900e-64	93.13	Pyruvate kinase OS=Populus trichocarpa GN=POPTRDRA...	[more]
D7UBT5_VITVI	2.455e-63	90.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BDS1_VITVI	2.455e-63	90.84	Pyruvate kinase OS=Vitis vinifera GN=VITISV_026975...	[more]
Q9LF93_ARATH	7.897e-62	90.77	Pyruvate kinase OS=Arabidopsis thaliana GN=F8J2_16...	[more]
Q94KE3_ARATH	7.897e-62	90.77	Pyruvate kinase OS=Arabidopsis thaliana GN=At3g529...	[more]
D7TIZ5_VITVI	7.897e-62	90.08	Whole genome shotgun sequence of line PN40024, sca...	[more]
A8MR07_ARATH	7.897e-62	90.77	Pyruvate kinase OS=Arabidopsis thaliana GN=AT3G529...	[more]
D7LUD2_ARALY	1.759e-61	90.77	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of GR915146 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G52990.2	4.848e-64	90.77	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G52990.1	4.848e-64	90.77	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT2G36580.1	2.252e-61	86.92	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G56350.1	8.084e-27	44.62	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G63680.1	2.352e-26	43.48	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT5G08570.1	3.072e-26	43.48	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT4G26390.1	6.844e-26	40.44	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G25960.1	9.883e-25	43.51	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G04050.1	1.291e-24	42.31	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]
AT3G55650.1	5.423e-23	40.77	\| Symbols: \| Pyruvate kinase family protein \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 53..67 score: 6.4e-27coord: 85..111 score: 6.4e-27coord: 112..136 score: 6.4e-27coord: 137..141 score: 6.4
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 11..141 score: 2.7
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 26..141 score: 5.8
IPR015813	Pyruvate/Phosphoenolpyruvate kinase	GENE3D	3.20.20.60	no description	coord: 21..141 score: 7.5
IPR015813	Pyruvate/Phosphoenolpyruvate kinase	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 24..141 score: 4.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR915146 ID=GR915146; Name=GR915146; organism=Cicer arietinum; type=EST; length=424bp
GATTTTCGAGCGGCCGCCCGGGCAGGTACTATAAAGAATTCAGCTACATT
GGCTGGGTCATTGTTCACTTTGCATGCCTCTCAAGTTCGTATTGAATTAC
CTACCCTTACAGAGAAAGACAAGGAGGTTATAAGCACCTGGGGTGTTAAG
AACAAAATTGATTTTCTGTCATTGTCATATACCCGGCATGCCGAAGACGT
CCGTGAGGCTCGTGAGTTCCTTTCAAAGTTAGGCGACCTTAGTCAGACTC
AAATTTTTGCAAAGATTGAAAATGTTGAGGGATTGACCCATTTTGATGAG
ATTCTGCAAGAAGCTGATGGTATTATCCTTTCCCGTGGGAATTTGGGCAT
TGATCTTCCACCAGAGAAGGTTTTTCTGTTTCAAAAATCTGCCCTATACA
AGTGTAATATGGCCGGGAAGCCTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0004743	pyruvate kinase activity
GO:0030955	potassium ion binding
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001697	Pyr_Knase
IPR015793	Pyrv_Knase_brl
IPR015813	Pyrv/PenolPyrv_Kinase-like_dom

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