GR917993

EST Overview


Name	GR917993
Unique Name	GR917993
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	405

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR917993 vs. SwissProt
Match: METB_ARATH (Cystathionine gamma-synthase, chloroplastic OS=Arabidopsis thaliana GN=CGS1 PE=2 SV=3)

HSP 1 Score: 194.126 bits (492), Expect = 1.864e-49
Identity = 92/127 (72.44%), Postives = 109/127 (85.83%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL +KIS LEGAEST++MASGMCAS V+L+ALVPAGGH+VTTTDCYRKTRIF+E  LPK+GIT TV+DPAD+  LE+A+ +  VSLFFTESPTNPFLRCVDI+LVS++CHK G L+CIDGT
Sbjct:  230 GNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCIDGT 356

[Close]

BLAST of GR917993 vs. SwissProt
Match: METZ_PSEAE (O-succinylhomoserine sulfhydrylase OS=Pseudomonas aeruginosa GN=metZ PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.032e-15
Identity = 44/116 (37.93%), Postives = 65/116 (56.03%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 384
            E+I+ LEGAE  V  ASGM A + L+M+L  +G H++ +   +  T    +    + GI       +D+ A E+A +  N  LFF ESP+NP    VDI  ++E+ H  GALL +D
Sbjct:   80 ERIAALEGAEQAVATASGMSAILALVMSLCSSGDHVLVSRSVFGSTISLFDKYFKRFGIQVDYPPLSDLAAWEAACKP-NTKLFFVESPSNPLAELVDIAALAEIAHAKGALLAVD 194

[Close]

BLAST of GR917993 vs. SwissProt
Match: MCCB_BACSU (Cystathionine gamma-lyase OS=Bacillus subtilis GN=mccB PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.336e-14
Identity = 44/122 (36.07%), Postives = 70/122 (57.38%), Query Frame = 1

Query:   25 TVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            T L   ++ELE  E+    +SGM A+I  +M L  +G H+V T D Y  T   +  VL ++GI +T VD +    +E A+  N  +++  E+PTNP L+  D+ L++++  K G LL +D T
Sbjct:   53 TALEALVTELESGEAGYAFSSGM-AAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYI-ETPTNPLLKITDLTLMADIAKKAGVLLIVDNT 172

[Close]

BLAST of GR917993 vs. SwissProt
Match: METC_RHIL3 (Putative cystathionine beta-lyase OS=Rhizobium leguminosarum bv. viciae (strain 3841) GN=metC PE=3 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 5.663e-14
Identity = 42/128 (32.81%), Postives = 66/128 (51.56%), Query Frame = 1

Query:    7 RGRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            RG      L E I  LEG+  T+L+ SG+ A  +  +  V AG H +     Y  TR F +T+L ++G+      P ++GA    L ++N  L  TE+P +      DI  +S + H+HGA++ +D T
Sbjct:   61 RGTPTTDALCEAIDALEGSAGTILVPSGLAAVTIPFLGFVAAGDHALVVDSVYGPTRHFCDTMLKRLGVEVEYYHP-EIGAGIETLFRSNTKLVHTEAPGSNTFEMQDIPAISAVAHRHGAVVMMDNT 187

[Close]

BLAST of GR917993 vs. SwissProt
Match: METC_LACLM (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=metC PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.822e-12
Identity = 40/118 (33.90%), Postives = 69/118 (58.47%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            E I++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   +VD +++  L++A ++   +++F E+P+NP L+ +DIK +S +   H AL  +D T
Sbjct:   58 ELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVDNT 173

[Close]

BLAST of GR917993 vs. SwissProt
Match: METC_LACLA (Cystathionine beta-lyase OS=Lactococcus lactis subsp. lactis GN=metC PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.822e-12
Identity = 40/118 (33.90%), Postives = 69/118 (58.47%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            E I++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   +VD +++  L++A ++   +++F E+P+NP L+ +DIK +S +   H AL  +D T
Sbjct:   58 ELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVDNT 173

[Close]

BLAST of GR917993 vs. SwissProt
Match: METC_LACLC (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris GN=metC PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.379e-12
Identity = 40/118 (33.90%), Postives = 68/118 (57.63%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            E I++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   +VD +++  L++A +    +++F E+P+NP L+ +DIK +S +   H AL  +D T
Sbjct:   58 ELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLVDKVLTKTGIIYDLVDLSNLEDLKAAFKAETKAVYF-ETPSNPLLKVLDIKEISSIAKAHNALTLVDNT 173

[Close]

BLAST of GR917993 vs. SwissProt
Match: METB_HELPY (Cystathionine gamma-synthase OS=Helicobacter pylori GN=metB PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.042e-12
Identity = 37/118 (31.36%), Postives = 65/118 (55.08%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            E I++LEG       ASG+ A I  + +L+ +G H++   D Y  T      VL K G++ T++D +D+  ++ A++ N  +L+  E+P+NP L+  D+   + +   HG L  +D T
Sbjct:   57 ELIADLEGGVKGFAFASGL-AGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNQVLVKNGLSCTIIDTSDISQIKKAIKPNTKALYL-ETPSNPLLKITDLAQCASVAKDHGLLTIVDNT 172

[Close]

BLAST of GR917993 vs. SwissProt
Match: CGL_PIG (Cystathionine gamma-lyase OS=Sus scrofa GN=CTH PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.861e-11
Identity = 38/128 (29.69%), Postives = 70/128 (54.69%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHG-ALLCIDGT 390
            G      L + ++ L+GA+ ++  ASG+ A++ +   L+ AG  +++  D Y  T  +   V  + G+  + VD +    LE+A+      L + E+PTNP L+ +DI+  +++ HKHG  +L +D T
Sbjct:   64 GNPTXNCLEKAVAVLDGAKYSLAFASGLAATVTITH-LLKAGXQIISMDDVYGGTNRYFRQVAAEFGLKISFVDCSKSKLLEAAITPET-KLVWIETPTNPILKMIDIEACAQIVHKHGDIILVVDNT 189

[Close]

BLAST of GR917993 vs. SwissProt
Match: MEGL_PSEPU (Methionine gamma-lyase OS=Pseudomonas putida GN=mdeA PE=1 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 7.654e-11
Identity = 39/117 (33.33%), Postives = 63/117 (53.85%), Query Frame = 1

Query:   40 KISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            +++ LEG E+ + +ASGM A    L  L+  G  ++     Y  T  F+   + + G+    VD AD+ ALE+A+      ++F ESP NP +   DI  V+++  KHGA + +D T
Sbjct:   73 RMASLEGGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGEFGVKLRHVDMADLQALEAAMTPATRVIYF-ESPANPNMHMADIAGVAKIARKHGATVVVDNT 188

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q0GC25_MEDSA (Cystathionine gamma-synthase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.062e-58
Identity = 114/127 (89.76%), Postives = 118/127 (92.91%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G    TVL EKISELEGAEST+LMASGMCAS+VL MALVPAGGHLVTTTDCYRKTRIFIET LPKMGITTTV+DPADVGALESALEQ+NVSLFFTESPTNPFLRCVDIKLVSELCHK G LLCIDGT
Sbjct:  203 GNPTSTVLEEKISELEGAESTILMASGMCASVVLFMALVPAGGHLVTTTDCYRKTRIFIETFLPKMGITTTVIDPADVGALESALEQHNVSLFFTESPTNPFLRCVDIKLVSELCHKKGTLLCIDGT 329

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q45NM6_MEDSA (Cystathionine-gamma-synthase (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 1.534e-57
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 1

Query:   25 TVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            TVL EKISELEGAEST+LMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGI T+VVDPADVGAL+SALEQN VSLFFTESPTNPFLRCVDIKLVSELCHK+GALL IDGT
Sbjct:    1 TVLEEKISELEGAESTILMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGIKTSVVDPADVGALQSALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKNGALLVIDGT 122

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q9XFG0_SOYBN (Cystathionine-gamma-synthase OS=Glycine max GN=cys PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 4.463e-57
Identity = 112/127 (88.19%), Postives = 116/127 (91.34%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL EKIS LEGAESTV+MASGMCAS+VL MALVPAGGHLVTTTDCYRKTRIFIET LPKMGITTTV+DPADVGALESALEQ+NVSLFFTESPTNPFLRCVDIKLVSELCHK G LLCIDGT
Sbjct:  203 GNPTTVVLEEKISALEGAESTVIMASGMCASVVLFMALVPAGGHLVTTTDCYRKTRIFIETFLPKMGITTTVIDPADVGALESALEQHNVSLFFTESPTNPFLRCVDIKLVSELCHKKGTLLCIDGT 329

[Close]

BLAST of GR917993 vs. TrEMBL
Match: O81334_MESCR (Cystathionine gamma-synthase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.029e-53
Identity = 103/127 (81.10%), Postives = 115/127 (90.55%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL +KIS LEGAEST+L+ASGMCASIVL++ALVPAGGH+VTTTDCYRKTRIFIET LPKMGIT TV+DPADV  L+SAL++NNV+LFFTESPTNPFLRCVDIKLVSELCHK GAL+CIDGT
Sbjct:  215 GNPTSVVLEDKISALEGAESTILLASGMCASIVLMLALVPAGGHIVTTTDCYRKTRIFIETFLPKMGITATVIDPADVEGLKSALDENNVTLFFTESPTNPFLRCVDIKLVSELCHKKGALVCIDGT 341

[Close]

BLAST of GR917993 vs. TrEMBL
Match: B9SVH8_RICCO (Cystathionine gamma-synthase, putative (Fragment) OS=Ricinus communis GN=RCOM_0537490 PE=3 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 2.994e-53
Identity = 101/118 (85.59%), Postives = 112/118 (94.92%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            EKIS LEGAEST++MASGMCAS V+L+ALVPAGGH+VTTTDCYRKTRIFIETVLPKMGIT TV+DPADV  LE+ALE+NNVSLFFTESPTNPFLRCVDIKLVSELCH+ GA++CIDGT
Sbjct:  101 EKISALEGAESTLIMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFIETVLPKMGITATVIDPADVEGLEAALEKNNVSLFFTESPTNPFLRCVDIKLVSELCHRKGAIVCIDGT 218

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q68YN6_MEDSA (Cystathionine gamma synthase (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 8.710e-53
Identity = 102/110 (92.73%), Postives = 106/110 (96.36%), Query Frame = 1

Query:   61 AESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            AESTV+MASGMCAS+VL MALVPAGGHLVTTTDCYRKTRIFIET LPKMGITTTV+DPADVGALESALEQ+NVSLFFTESPTNPFLRCVDIKLVSELCHK G LLCIDGT
Sbjct:    1 AESTVIMASGMCASVVLFMALVPAGGHLVTTTDCYRKTRIFIETFLPKMGITTTVIDPADVGALESALEQHNVSLFFTESPTNPFLRCVDIKLVSELCHKKGTLLCIDGT 110

[Close]

BLAST of GR917993 vs. TrEMBL
Match: B9IK07_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_835709 PE=3 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.138e-52
Identity = 103/121 (85.12%), Postives = 113/121 (93.39%), Query Frame = 1

Query:   28 VL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            VL +KISELEGAEST+++ASGMCAS VLL+ALVPAGGHLVTTTDCYRKTRIF+ET LPKMGIT TV+DPADV ALESALE N VSLFFTESPTNPFLRCVDI+LVSELCH+ GAL+CIDGT
Sbjct:  202 VLEDKISELEGAESTLILASGMCASTVLLLALVPAGGHLVTTTDCYRKTRIFMETFLPKMGITVTVIDPADVKALESALEINKVSLFFTESPTNPFLRCVDIELVSELCHRKGALVCIDGT 322

[Close]

BLAST of GR917993 vs. TrEMBL
Match: O23944_FRAVE (Cystathionine gamma synthase OS=Fragaria vesca GN=CGS PE=2 SV=2)

HSP 1 Score: 208.379 bits (529), Expect = 1.940e-52
Identity = 101/127 (79.53%), Postives = 113/127 (88.98%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL EKIS LEGAEST+L+ASGMCAS VLLMALVPAGGH+VTTTDCYRKTRIFIET+LPKMGIT T++DPADVGALE+AL +N VSLFFTESPTNPFLRCVDI LVS+LCH  GA++CI+GT
Sbjct:  212 GNPTTVVLEEKISALEGAESTLLLASGMCASTVLLMALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATIIDPADVGALETALNENKVSLFFTESPTNPFLRCVDINLVSDLCHSKGAVVCINGT 338

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q9ZPL5_TOBAC (Cystathionine gamma-synthase OS=Nicotiana tabacum GN=metB PE=1 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.534e-52
Identity = 102/127 (80.31%), Postives = 113/127 (88.98%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL EKIS LEGAEST+LMASGMCAS V+L+ALVPAGGH+VTTTDCYRKTRIFIET+LPKMGIT TV+DPADVGALE AL Q  V+LFFTESPTNPFLRCVDI+LVS+LCH+ GAL+CIDGT
Sbjct:  112 GNPTTVVLEEKISALEGAESTLLMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATVIDPADVGALELALNQKKVNLFFTESPTNPFLRCVDIELVSKLCHEKGALVCIDGT 238

[Close]

BLAST of GR917993 vs. TrEMBL
Match: Q9MT29_SOLTU (Cystathionine gamma-synthase isoform 2 OS=Solanum tuberosum GN=CgS2 PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.643e-51
Identity = 99/127 (77.95%), Postives = 112/127 (88.19%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     V  EKIS LEGA++T+LMASGMCAS VLL+ALVPAGGH+VTTTDCYRKTRIFIET+LPKMGIT TV+DPAD+GALES L Q  V+LFFTESPTNPFLRCVDI+LVS+LCH+ GAL+CIDGT
Sbjct:  207 GNPTSIVAEEKISALEGADATLLMASGMCASTVLLLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATVIDPADIGALESVLNQKKVTLFFTESPTNPFLRCVDIELVSKLCHEKGALVCIDGT 333

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT3G01120.1 (| Symbols: MTO1, CGS, ATCYS1, CGS1 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr3:39234-41865 REVERSE LENGTH=563)

HSP 1 Score: 194.126 bits (492), Expect = 2.114e-50
Identity = 92/127 (72.44%), Postives = 109/127 (85.83%), Query Frame = 1

Query:   10 GRGRGTVL*EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            G     VL +KIS LEGAEST++MASGMCAS V+L+ALVPAGGH+VTTTDCYRKTRIF+E  LPK+GIT TV+DPAD+  LE+A+ +  VSLFFTESPTNPFLRCVDI+LVS++CHK G L+CIDGT
Sbjct:  230 GNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCIDGT 356

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT1G33320.1 (| Symbols: | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr1:12081009-12083462 FORWARD LENGTH=412)

HSP 1 Score: 162.54 bits (410), Expect = 6.815e-41
Identity = 76/118 (64.41%), Postives = 95/118 (80.51%), Query Frame = 1

Query:   37 EKISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            EKIS LEGAEST++MASGM AS V+L+ALVP  GH+V T DCY++TRIF+E  L K+GIT T +D  D+  L++ +  + VSLFFTESPTNPFLRCVDIKLVS++CH+ G L+CID T
Sbjct:   89 EKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCYKETRIFMENFLTKLGITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLVSKICHRRGTLVCIDAT 206

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT1G64660.1 (| Symbols: ATMGL, MGL | methionine gamma-lyase | chr1:24028977-24030537 FORWARD LENGTH=441)

HSP 1 Score: 70.0922 bits (170), Expect = 4.603e-13
Identity = 37/118 (31.36%), Postives = 63/118 (53.39%), Query Frame = 1

Query:   40 KISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKM-GITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            +++ LEG ++    +SGM A   +++ L  +GGH+V  +  Y  T   +   LP+   ITT+ VD  D GA+ +A+ +    + + ES  NP L   DI  +S + H+ G  + +D T
Sbjct:  109 QMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTVVVDNT 226

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT3G57050.2 (| Symbols: CBL | cystathionine beta-lyase | chr3:21111939-21114521 REVERSE LENGTH=449)

HSP 1 Score: 54.299 bits (129), Expect = 2.614e-8
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 1

Query:   43 ISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            +++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    V+   +  + +A+      L + ESPTNP  +  DI+ +SE+ H  GAL+ +D +
Sbjct:  127 LAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGPQT-KLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNS 240

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT3G57050.3 (| Symbols: CBL | cystathionine beta-lyase | chr3:21112392-21114521 REVERSE LENGTH=378)

HSP 1 Score: 54.299 bits (129), Expect = 2.614e-8
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 1

Query:   43 ISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            +++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    V+   +  + +A+      L + ESPTNP  +  DI+ +SE+ H  GAL+ +D +
Sbjct:  142 LAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGPQT-KLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNS 255

[Close]

BLAST of GR917993 vs. TAIR peptide
Match: AT3G57050.1 (| Symbols: CBL | cystathionine beta-lyase | chr3:21111939-21114521 REVERSE LENGTH=464)

HSP 1 Score: 54.299 bits (129), Expect = 2.614e-8
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 1

Query:   43 ISELEGAESTVLMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITTTVVDPADVGALESALEQNNVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCIDGT 390
            +++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    V+   +  + +A+      L + ESPTNP  +  DI+ +SE+ H  GAL+ +D +
Sbjct:  142 LAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGPQT-KLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNS 255

The following BLAST results are available for this feature:

BLAST of GR917993 vs. SwissProt
BLAST of GR917993 vs. TrEMBL
BLAST of GR917993 vs. TAIR peptide

BLAST of GR917993 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
METB_ARATH	1.864e-49	72.44	Cystathionine gamma-synthase, chloroplastic OS=Ara...	[more]
METZ_PSEAE	1.032e-15	37.93	O-succinylhomoserine sulfhydrylase OS=Pseudomonas ...	[more]
MCCB_BACSU	4.336e-14	36.07	Cystathionine gamma-lyase OS=Bacillus subtilis GN=...	[more]
METC_RHIL3	5.663e-14	32.81	Putative cystathionine beta-lyase OS=Rhizobium leg...	[more]
METC_LACLM	1.822e-12	33.90	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METC_LACLA	1.822e-12	33.90	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METC_LACLC	2.379e-12	33.90	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METB_HELPY	9.042e-12	31.36	Cystathionine gamma-synthase OS=Helicobacter pylor...	[more]
CGL_PIG	5.861e-11	29.69	Cystathionine gamma-lyase OS=Sus scrofa GN=CTH PE=...	[more]
MEGL_PSEPU	7.654e-11	33.33	Methionine gamma-lyase OS=Pseudomonas putida GN=md...	[more]

BLAST of GR917993 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0GC25_MEDSA	1.062e-58	89.76	Cystathionine gamma-synthase OS=Medicago sativa PE...	[more]
Q45NM6_MEDSA	1.534e-57	93.44	Cystathionine-gamma-synthase (Fragment) OS=Medicag...	[more]
Q9XFG0_SOYBN	4.463e-57	88.19	Cystathionine-gamma-synthase OS=Glycine max GN=cys...	[more]
O81334_MESCR	1.029e-53	81.10	Cystathionine gamma-synthase OS=Mesembryanthemum c...	[more]
B9SVH8_RICCO	2.994e-53	85.59	Cystathionine gamma-synthase, putative (Fragment) ...	[more]
Q68YN6_MEDSA	8.710e-53	92.73	Cystathionine gamma synthase (Fragment) OS=Medicag...	[more]
B9IK07_POPTR	1.138e-52	85.12	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
O23944_FRAVE	1.940e-52	79.53	Cystathionine gamma synthase OS=Fragaria vesca GN=...	[more]
Q9ZPL5_TOBAC	2.534e-52	80.31	Cystathionine gamma-synthase OS=Nicotiana tabacum ...	[more]
Q9MT29_SOLTU	1.643e-51	77.95	Cystathionine gamma-synthase isoform 2 OS=Solanum ...	[more]

BLAST of GR917993 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G01120.1	2.114e-50	72.44	\| Symbols: MTO1, CGS, ATCYS1, CGS1 \| Pyridoxal pho...	[more]
AT1G33320.1	6.815e-41	64.41	\| Symbols: \| Pyridoxal phosphate (PLP)-dependent ...	[more]
AT1G64660.1	4.603e-13	31.36	\| Symbols: ATMGL, MGL \| methionine gamma-lyase \| c...	[more]
AT3G57050.2	2.614e-8	27.59	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]
AT3G57050.3	2.614e-8	27.59	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]
AT3G57050.1	2.614e-8	27.59	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 26..118 score: 7.2
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 1..119 score: 3.8
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 1..119 score: 1.1
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 1..118 score: 2.9
None	No IPR available	PANTHER	PTHR11808:SF14	CYSTATHIONINE GAMMA-SYNTHASE	coord: 26..118 score: 7.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR917993 ID=GR917993; Name=GR917993; organism=Cicer arietinum; type=EST; length=405bp
GATTAGCGTGGTCGCGGCCGAGGTACTGTTTTATAGGAGAAGATAAGTGA
ACTCGAAGGAGCTGAATCAACTGTCTTAATGGCATCTGGAATGTGTGCTA
GCATAGTCTTGTTGATGGCGCTGGTTCCAGCTGGTGGACATCTTGTGACT
ACTACAGATTGTTACCGGAAGACTAGGATTTTTATAGAGACTGTGCTTCC
AAAGATGGGGATCACGACCACCGTGGTTGATCCTGCTGATGTTGGAGCCT
TGGAATCTGCATTGGAGCAGAACAATGTGTCTCTTTTCTTCACTGAGTCT
CCTACCAATCCATTCCTGAGATGTGTTGACATTAAGCTGGTTTCAGAGCT
TTGCCATAAGCATGGGGCTTTGCTCTGTATTGATGGTACCTGCCCGGGCG
GCCGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006520	amino acid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0030170	pyridoxal phosphate binding
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000277	Cys/Met-Metab_PyrdxlP-dep_enz
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

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