HO062316

EST Overview


Name	HO062316
Unique Name	HO062316
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	708

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC34_ARATH (Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana GN=UBC34 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.198e-28
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 26.9498 bits (58), Expect = 1.198e-28
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   I+MT      MTQKKICL
Sbjct:   64 YPYKPPGITMTTPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 1.198e-28
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC33_ARATH (Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana GN=UBC33 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.238e-26
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 24.6386 bits (52), Expect = 1.238e-26
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. SwissProt
Match: UB2JL_DICDI (Ubiquitin-conjugating enzyme E2 J2-like OS=Dictyostelium discoideum GN=DDB_G0294380 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.023e-11
Identity = 28/57 (49.12%), Postives = 41/57 (71.93%), Query Frame = 1

Query:  316 KACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            K CI RL++E++ + K P+ +I+  PSPS+ILEWHYV+ G+  TP+  G YYG+  F
Sbjct:   14 KDCITRLKREFQEISKNPIENILVTPSPSNILEWHYVILGASNTPYEGGVYYGQLIF 70

[Close]

BLAST of HO062316 vs. SwissProt
Match: UB2J2_MOUSE (Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus GN=Ube2j2 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.632e-11
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 1

Query:  310 AEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            A     +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+  GYY+GK  F
Sbjct:    9 APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIF 67

[Close]

BLAST of HO062316 vs. SwissProt
Match: UB2J2_HUMAN (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 6.632e-11
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 1

Query:  310 AEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            A     +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+  GYY+GK  F
Sbjct:    9 APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIF 67

[Close]

BLAST of HO062316 vs. SwissProt
Match: UB2J2_BOVIN (Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus GN=UBE2J2 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.632e-11
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 1

Query:  310 AEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            A     +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+  GYY+GK  F
Sbjct:    9 APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIF 67

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC6_SCHPO (Ubiquitin-conjugating enzyme E2 6 OS=Schizosaccharomyces pombe GN=ubc6 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.333e-10
Identity = 29/60 (48.33%), Postives = 37/60 (61.67%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MA K   KRL KEY AL K PV  + A+P+  +ILEWHY++ G   TP+  G Y+G   F
Sbjct:    1 MASKGAYKRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIF 60

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC6_CANGA (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata GN=UBC6 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.333e-10
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MA K   KRL KEY+ + + P   I+ARP+  +ILEWHYV+ G   TP+  G Y+G  +F
Sbjct:    1 MATKQAQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTF 60

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC6_YEAST (Ubiquitin-conjugating enzyme E2 6 OS=Saccharomyces cerevisiae GN=UBC6 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 9.577e-10
Identity = 27/60 (45.00%), Postives = 39/60 (65.00%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MA K   KRL KEY+ + + P  +I+ARP+  +ILEWHY++ G   TP+  G Y+G  +F
Sbjct:    1 MATKQAHKRLTKEYKLMVENPPPYILARPNEDNILEWHYIITGPADTPYKGGQYHGTLTF 60

[Close]

BLAST of HO062316 vs. SwissProt
Match: UBC6_DEBHA (Ubiquitin-conjugating enzyme E2 6 OS=Debaryomyces hansenii GN=UBC6 PE=3 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 4.753e-9
Identity = 26/56 (46.43%), Postives = 36/56 (64.29%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYG 474
            MA +   KRL KEY+A+   P  ++ A+P+  +ILEWHYV+ G   TPF  G Y+G
Sbjct:    1 MASRQSQKRLTKEYKAITLNPPPYVSAKPNDENILEWHYVITGPPHTPFEEGQYHG 56

[Close]

BLAST of HO062316 vs. TrEMBL
Match: B7FML7_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.234e-28
Identity = 57/62 (91.94%), Postives = 57/62 (91.94%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSFLQ 492
            MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSE TPFS GYYYGK  F Q
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFSGGYYYGKIKFPQ 62

HSP 2 Score: 28.1054 bits (61), Expect = 2.234e-28
Identity = 16/25 (64.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   ISMT      MTQKKICL
Sbjct:   64 YPYKPPGISMTTPNGRFMTQKKICL 88

HSP 3 Score: 21.557 bits (44), Expect = 2.234e-28
Identity = 8/14 (57.14%), Postives = 9/14 (64.29%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFP +    PPG
Sbjct:   57 KIKFPQEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: C6TMV6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 2.903e-28
Identity = 53/60 (88.33%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+CIKRLQKEYRALCKEPVSH+VARPSPSDILEWHYVLEGSE TPF+ GYYYGK  F
Sbjct:    1 MAEKSCIKRLQKEYRALCKEPVSHVVARPSPSDILEWHYVLEGSEGTPFAGGYYYGKIKF 60

HSP 2 Score: 28.1054 bits (61), Expect = 2.903e-28
Identity = 16/25 (64.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   ISMT      MTQKKICL
Sbjct:   64 YPYKPPGISMTTPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 2.903e-28
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: D7TZJ5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016663001 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 8.159e-28
Identity = 51/60 (85.00%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+C+KRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ GYYYGK  F
Sbjct:   69 MAEKSCVKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGYYYGKIKF 128

HSP 2 Score: 28.1054 bits (61), Expect = 8.159e-28
Identity = 16/25 (64.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   ISMT      MTQKKICL
Sbjct:  132 YPYKPPGISMTTPNGRFMTQKKICL 156

HSP 3 Score: 24.6386 bits (52), Expect = 8.159e-28
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:  125 KIKFPPEYPYKPPG 138

[Close]

BLAST of HO062316 vs. TrEMBL
Match: D7KFD7_ARALY (Ubiquitin-conjugating enzyme 32 OS=Arabidopsis lyrata subsp. lyrata GN=UBC34 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.806e-27
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 26.9498 bits (58), Expect = 1.806e-27
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   I+MT      MTQKKICL
Sbjct:   64 YPYKPPGITMTTPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 1.806e-27
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: Q94FU3_SOLLC (Ubiquitin conjugating enzyme 2 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.343e-27
Identity = 51/60 (85.00%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKAC+KRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACVKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 26.5646 bits (57), Expect = 2.343e-27
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YP+K   ISMT      MTQKKICL
Sbjct:   64 YPFKPPGISMTTPNGRFMTQKKICL 88

HSP 3 Score: 25.0238 bits (53), Expect = 2.343e-27
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPFKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: B9MUK3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_589822 PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.345e-27
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+C+KRLQKEYRALCKEPVSH+VARPSPSDILEWHYVLEGSE TPF+ GYYYGK  F
Sbjct:    1 MAEKSCVKRLQKEYRALCKEPVSHVVARPSPSDILEWHYVLEGSEGTPFAGGYYYGKIKF 60

HSP 2 Score: 25.409 bits (54), Expect = 2.345e-27
Identity = 15/25 (60.00%), Postives = 15/25 (60.00%), Query Frame = 2

Query:  494 YPYKSSRISM-THL*AIMTQKKICL 565
            YPYK   I M T     MTQKKICL
Sbjct:   64 YPYKPPGIIMITPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 2.345e-27
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: B9T6E8_RICCO (Ubiquitin-conjugating enzyme E2 j2, putative OS=Ricinus communis GN=RCOM_0333490 PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.174e-25
Identity = 48/60 (80.00%), Postives = 52/60 (86.67%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+C+ RLQKEYRALCKEPV H+VARPS  DILEWHYVLEGSE TPF+ GYYYGK  F
Sbjct:    1 MAEKSCVSRLQKEYRALCKEPVPHVVARPSSRDILEWHYVLEGSEGTPFAGGYYYGKIKF 60

HSP 2 Score: 28.4906 bits (62), Expect = 1.174e-25
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPPD   NPPG
Sbjct:   57 KIKFPPDYPYNPPG 70

HSP 3 Score: 24.6386 bits (52), Expect = 1.174e-25
Identity = 14/25 (56.00%), Postives = 15/25 (60.00%), Query Frame = 2

Query:  494 YPYKSSRISM-THL*AIMTQKKICL 565
            YPY    I+M T     MTQKKICL
Sbjct:   64 YPYNPPGITMITPNGRFMTQKKICL 88

[Close]

BLAST of HO062316 vs. TrEMBL
Match: A5C2P5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027501 PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.798e-25
Identity = 51/60 (85.00%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+C+KRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ GYYYGK  F
Sbjct:  872 MAEKSCVKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGYYYGKIKF 931

HSP 2 Score: 24.6386 bits (52), Expect = 1.798e-25
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:  928 KIKFPPEYPYKPPG 941

[Close]

BLAST of HO062316 vs. TrEMBL
Match: D7MQ79_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495109 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.178e-24
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCK PVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKVPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 24.6386 bits (52), Expect = 1.178e-24
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TrEMBL
Match: B9GY01_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_830711 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 3.178e-24
Identity = 51/60 (85.00%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEK+C+KRLQKEYRALCKEPVSH+VARPSPSDILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKSCVKRLQKEYRALCKEPVSHVVARPSPSDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

[Close]

BLAST of HO062316 vs. TAIR peptide
Match: AT1G17280.2 (| Symbols: UBC34 | ubiquitin-conjugating enzyme 34 | chr1:5917146-5919162 REVERSE LENGTH=237)

HSP 1 Score: 116.316 bits (290), Expect = 1.208e-29
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 26.9498 bits (58), Expect = 1.208e-29
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   I+MT      MTQKKICL
Sbjct:   64 YPYKPPGITMTTPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 1.208e-29
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TAIR peptide
Match: AT1G17280.1 (| Symbols: UBC34 | ubiquitin-conjugating enzyme 34 | chr1:5917146-5919162 REVERSE LENGTH=237)

HSP 1 Score: 116.316 bits (290), Expect = 1.208e-29
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 26.9498 bits (58), Expect = 1.208e-29
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 2

Query:  494 YPYKSSRISMTHL*A-IMTQKKICL 565
            YPYK   I+MT      MTQKKICL
Sbjct:   64 YPYKPPGITMTTPNGRFMTQKKICL 88

HSP 3 Score: 24.6386 bits (52), Expect = 1.208e-29
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TAIR peptide
Match: AT5G50430.2 (| Symbols: UBC33 | ubiquitin-conjugating enzyme 33 | chr5:20534366-20536307 REVERSE LENGTH=243)

HSP 1 Score: 116.316 bits (290), Expect = 1.252e-27
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 24.6386 bits (52), Expect = 1.252e-27
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TAIR peptide
Match: AT5G50430.1 (| Symbols: UBC33 | ubiquitin-conjugating enzyme 33 | chr5:20534366-20536307 REVERSE LENGTH=243)

HSP 1 Score: 116.316 bits (290), Expect = 1.252e-27
Identity = 52/60 (86.67%), Postives = 56/60 (93.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSE TPF+ G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

HSP 2 Score: 24.6386 bits (52), Expect = 1.252e-27
Identity = 9/14 (64.29%), Postives = 10/14 (71.43%), Query Frame = 3

Query:  474 KIKFPPDIHINPPG 515
            KIKFPP+    PPG
Sbjct:   57 KIKFPPEYPYKPPG 70

[Close]

BLAST of HO062316 vs. TAIR peptide
Match: AT5G50430.3 (| Symbols: UBC33 | ubiquitin-conjugating enzyme 33 | chr5:20534366-20536307 REVERSE LENGTH=230)

HSP 1 Score: 83.9593 bits (206), Expect = 9.075e-17
Identity = 41/60 (68.33%), Postives = 44/60 (73.33%), Query Frame = 1

Query:  307 MAEKACIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSERTPFSNGYYYGKSSF 486
            MAEKACIKRLQKEYRALCKEPVSH+VARPSP+DILEW              G+YYGK  F
Sbjct:    1 MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEW-------------RGFYYGKIKF 47

The following BLAST results are available for this feature:

BLAST of HO062316 vs. SwissProt
BLAST of HO062316 vs. TrEMBL
BLAST of HO062316 vs. TAIR peptide

BLAST of HO062316 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
UBC34_ARATH	1.198e-28	86.67	Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis ...	[more]
UBC33_ARATH	1.238e-26	86.67	Probable ubiquitin-conjugating enzyme E2 33 OS=Ara...	[more]
UB2JL_DICDI	1.023e-11	49.12	Ubiquitin-conjugating enzyme E2 J2-like OS=Dictyos...	[more]
UB2J2_MOUSE	6.632e-11	49.15	Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus...	[more]
UB2J2_HUMAN	6.632e-11	49.15	Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens...	[more]
UB2J2_BOVIN	6.632e-11	49.15	Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus G...	[more]
UBC6_SCHPO	7.333e-10	48.33	Ubiquitin-conjugating enzyme E2 6 OS=Schizosacchar...	[more]
UBC6_CANGA	7.333e-10	46.67	Ubiquitin-conjugating enzyme E2 6 OS=Candida glabr...	[more]
UBC6_YEAST	9.577e-10	45.00	Ubiquitin-conjugating enzyme E2 6 OS=Saccharomyces...	[more]
UBC6_DEBHA	4.753e-9	46.43	Ubiquitin-conjugating enzyme E2 6 OS=Debaryomyces ...	[more]

BLAST of HO062316 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FML7_MEDTR	2.234e-28	91.94	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TMV6_SOYBN	2.903e-28	88.33	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TZJ5_VITVI	8.159e-28	85.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KFD7_ARALY	1.806e-27	86.67	Ubiquitin-conjugating enzyme 32 OS=Arabidopsis lyr...	[more]
Q94FU3_SOLLC	2.343e-27	85.00	Ubiquitin conjugating enzyme 2 OS=Solanum lycopers...	[more]
B9MUK3_POPTR	2.345e-27	86.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T6E8_RICCO	1.174e-25	80.00	Ubiquitin-conjugating enzyme E2 j2, putative OS=Ri...	[more]
A5C2P5_VITVI	1.798e-25	85.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7MQ79_ARALY	1.178e-24	85.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9GY01_POPTR	3.178e-24	85.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of HO062316 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G17280.2	1.208e-29	86.67	\| Symbols: UBC34 \| ubiquitin-conjugating enzyme 34...	[more]
AT1G17280.1	1.208e-29	86.67	\| Symbols: UBC34 \| ubiquitin-conjugating enzyme 34...	[more]
AT5G50430.2	1.252e-27	86.67	\| Symbols: UBC33 \| ubiquitin-conjugating enzyme 33...	[more]
AT5G50430.1	1.252e-27	86.67	\| Symbols: UBC33 \| ubiquitin-conjugating enzyme 33...	[more]
AT5G50430.3	9.075e-17	68.33	\| Symbols: UBC33 \| ubiquitin-conjugating enzyme 33...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 16..35 score
None	No IPR available	PANTHER	PTHR11621	UBIQUITIN-CONJUGATING ENZYME E2	coord: 2..63 score: 6.2
None	No IPR available	PANTHER	PTHR11621:SF14	UBIQUITIN-CONJUGATING ENZYME E2 J2	coord: 2..63 score: 6.2
None	No IPR available	SEG	seg	seg	coord: 1..14 score
IPR000608	Ubiquitin-conjugating enzyme, E2	PFAM	PF00179	UQ_con	coord: 12..63 score: 3.9
IPR000608	Ubiquitin-conjugating enzyme, E2	PROFILE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 11..63 score: 8
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	no description	coord: 4..63 score: 5.4
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 8..60 score: 9.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062316 ID=HO062316; Name=HO062316; organism=Cicer arietinum; type=EST; length=708bp
AGGTACGCGGGGATATCAATAATCTATTTTCTACAAAAAATTGGAAACGC
ACTTCTAAACTCACTCTACTCTCTCTCTCTCTCTCTCTTTCTATTCAACG
CCGCCGTTTTGGAACTCTTCATTTCGCTGTTTCGGTTGCCGAATAGGGGC
AATTAATTCACGCGGAAATACACGATTTTGCCACCAATTTTTTCTTCTGC
GGATCAAATTGGGGACTACAACAAATCGGATCTCAGGAAATTTATTTTGA
GAATAAAGAACCAGAGTATAGTGTAGTCCTTCTCAATACTGCTCTGAGTT
AGTTTGATGGCGGAGAAAGCATGCATCAAGCGCCTTCAGAAGGAGTATAG
AGCCCTTTGTAAAGAGCCAGTTTCCCATATTGTGGCTCGTCCTTCACCAA
GTGATATTCTTGAGTGGCATTACGTTTTGGAAGGAAGCGAGAGAACACCT
TTTTCAAATGGATATTACTATGGAAAATCAAGTTTCCTCCAGATATCCAT
ATAAATCCTCCAGGATCAGCATGACACACCTATGAGCGATCATGACCCAG
AAAAAGATCTGTTTATCTATGATGATTTCATCCTGAAGTCGATCCATGTA
GTCTGTATCAGCATACTACTGACTCTCATCTGATGACCTAGTCCACCCAG
ATGTAATCCCCTACTGTGAAGCCCGCTCAGCAATTTCCTTGCTTCATGAA
ATGCCACT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016881	acid-amino acid ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0043687	post-translational protein modification

Vocabulary: **INTERPRO**
Term	Definition
IPR000608	UBQ-conjugat_E2
IPR016135	UBQ-conjugating_enzyme/RWD

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