HO066950

EST Overview


Name	HO066950
Unique Name	HO066950
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	413

Libraries


Library Name	Type
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO066950 vs. SwissProt
Match: AB19A_PRUPE (Auxin-binding protein ABP19a OS=Prunus persica GN=ABP19A PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.413e-18
Identity = 42/53 (79.25%), Postives = 47/53 (88.68%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NGGG+  LAF SFSSP+PGLQILDFALFK+D PTELI  TTF+DAA
Sbjct:  144 GLLHFQVNGGGTPALAFPSFSSPSPGLQILDFALFKNDLPTELIAQTTFLDAA 196

[Close]

BLAST of HO066950 vs. SwissProt
Match: ABP20_PRUPE (Auxin-binding protein ABP20 OS=Prunus persica GN=ABP20 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.747e-15
Identity = 38/53 (71.70%), Postives = 44/53 (83.02%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NGG +  LAF SF S +PGLQILDFALFK+D PTE+I  TTF+DAA
Sbjct:  149 GLLHFQVNGGEAPALAFASFGSASPGLQILDFALFKNDLPTEVIAQTTFLDAA 201

[Close]

BLAST of HO066950 vs. SwissProt
Match: AB19B_PRUPE (Auxin-binding protein ABP19b OS=Prunus persica GN=ABP19B PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.931e-14
Identity = 36/52 (69.23%), Postives = 43/52 (82.69%), Query Frame = -2

Query:  230 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDA 385
            GLLHFQ+NGG +  +AF SFSSP+PGLQI+DFALFK+D  TELI  TT +DA
Sbjct:  144 GLLHFQVNGGDTPAIAFPSFSSPSPGLQIVDFALFKNDLATELIAQTTLLDA 195

[Close]

BLAST of HO066950 vs. SwissProt
Match: GL31_ARATH (Germin-like protein subfamily 3 member 1 OS=Arabidopsis thaliana GN=GLP1 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.135e-13
Identity = 35/52 (67.31%), Postives = 41/52 (78.85%), Query Frame = -2

Query:  230 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDA 385
            GLLHFQIN G S+  A V+F+S NPGLQILDFALF +  PTEL+  TTF+DA
Sbjct:  143 GLLHFQINAGKSSASAVVTFNSANPGLQILDFALFANSLPTELVVGTTFLDA 194

[Close]

BLAST of HO066950 vs. SwissProt
Match: GL33_ARATH (Germin-like protein subfamily 3 member 3 OS=Arabidopsis thaliana GN=GER3 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.259e-12
Identity = 33/53 (62.26%), Postives = 40/53 (75.47%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+N G    LAFV+F S +PGLQIL FALF +D P+EL+ ATTF+  A
Sbjct:  146 GLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSELVEATTFLSDA 198

[Close]

BLAST of HO066950 vs. SwissProt
Match: GLP1_SINAL (Germin-like protein 1 OS=Sinapis alba GN=GLP1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.970e-12
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = -2

Query:  236 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFI 385
            GLLHFQ+N G    LAFV+F S +PGLQIL FALF +D P+EL+ ATTF+
Sbjct:  146 GLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSELVEATTFL 195

[Close]

BLAST of HO066950 vs. SwissProt
Match: GLP1_BRANA (Germin-like protein 1 OS=Brassica napus GN=GER1 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.610e-11
Identity = 33/53 (62.26%), Postives = 40/53 (75.47%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQIN G +   A V+FSS +PGLQILDFALF +   TEL++ATTF+  A
Sbjct:  142 GLLHFQINAGKTPAAALVTFSSASPGLQILDFALFANTLSTELVSATTFLPPA 194

[Close]

BLAST of HO066950 vs. SwissProt
Match: GL814_ORYSJ (Germin-like protein 8-14 OS=Oryza sativa subsp. japonica GN=GER5 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.769e-10
Identity = 28/53 (52.83%), Postives = 38/53 (71.70%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLH+Q N G S  +A V+FS PNPGLQI D+ALF ++ P+ ++   TF+D A
Sbjct:  148 GLLHYQYNAGQSAAVALVAFSGPNPGLQITDYALFANNLPSAIVEKVTFLDDA 200

[Close]

BLAST of HO066950 vs. SwissProt
Match: GL813_ORYSJ (Germin-like protein 8-13 OS=Oryza sativa subsp. japonica GN=Os08g0459700 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.432e-9
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ N G +  +AF ++SS NPGLQILD+ALF ++ PT  +   TF+  A
Sbjct:  154 GLLHFQYNTGDTTAVAFAAYSSSNPGLQILDYALFANNLPTSYVVKGTFLAEA 206

[Close]

BLAST of HO066950 vs. TrEMBL
Match: D4NXQ4_ARAHY (Germin-like protein subfamily 3 member 4 OS=Arachis hypogaea GN=GLP5 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.065e-18
Identity = 46/53 (86.79%), Postives = 49/53 (92.45%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NGGG+  LAFVSFSS NPGLQILDFALFKSDFPTELIT TTF+DAA
Sbjct:  146 GLLHFQLNGGGTQALAFVSFSSANPGLQILDFALFKSDFPTELITQTTFLDAA 198

[Close]

BLAST of HO066950 vs. TrEMBL
Match: Q9AR81_PEA (Germin-like protein OS=Pisum sativum GN=glp3 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.817e-18
Identity = 47/52 (90.38%), Postives = 48/52 (92.31%), Query Frame = -2

Query:  230 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDA 385
            GLLHFQIN G SN LAFVSFSS NPGLQILDFALFKSDFPTELITATTF+DA
Sbjct:  146 GLLHFQINSGRSNALAFVSFSSANPGLQILDFALFKSDFPTELITATTFLDA 197

[Close]

BLAST of HO066950 vs. TrEMBL
Match: O49924_PEA (Germin-like protein (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.817e-18
Identity = 47/52 (90.38%), Postives = 48/52 (92.31%), Query Frame = -2

Query:  230 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDA 385
            GLLHFQIN G SN LAFVSFSS NPGLQILDFALFKSDFPTELITATTF+DA
Sbjct:   45 GLLHFQINSGRSNALAFVSFSSANPGLQILDFALFKSDFPTELITATTFLDA 96

[Close]

BLAST of HO066950 vs. TrEMBL
Match: C7S8C4_SOYBN (Germin-like protein 10 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.009e-17
Identity = 46/53 (86.79%), Postives = 47/53 (88.68%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQIN G S  LAFVSFSS NPGLQILDF+LFKSDFPTELIT TTFIDAA
Sbjct:  148 GLLHFQINDGESQALAFVSFSSANPGLQILDFSLFKSDFPTELITQTTFIDAA 200

[Close]

BLAST of HO066950 vs. TrEMBL
Match: D1FMZ3_9ROSA (Putative auxin-binding protein 2 OS=Prunus salicina GN=ABP2 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.102e-15
Identity = 41/53 (77.36%), Postives = 46/53 (86.79%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NGGG+  LAF SFSSP+PGLQILDFALFK+D  TELI  TTF+DAA
Sbjct:  144 GLLHFQVNGGGTPALAFPSFSSPSPGLQILDFALFKNDLATELIAQTTFLDAA 196

[Close]

BLAST of HO066950 vs. TrEMBL
Match: C6K8P0_9ROSA (Auxin-binding protein ABP19 (Fragment) OS=Prunus incisa PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.219e-14
Identity = 39/53 (73.58%), Postives = 46/53 (86.79%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NGGG+  LAF SFSSP+PGLQILDFALF+++  TELI  TTF+DAA
Sbjct:    7 GLLHFQVNGGGTPALAFPSFSSPSPGLQILDFALFENNLATELIAQTTFLDAA 59

[Close]

BLAST of HO066950 vs. TrEMBL
Match: B9RRB7_RICCO (Auxin-binding protein ABP19a, putative OS=Ricinus communis GN=RCOM_0712130 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.219e-14
Identity = 39/53 (73.58%), Postives = 44/53 (83.02%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQIN GGS+ LAFVSFSSP PGLQI DFALF +D P+EL+  TTF+D A
Sbjct:  143 GLLHFQINSGGSSALAFVSFSSPRPGLQITDFALFANDLPSELVEKTTFLDDA 195

[Close]

BLAST of HO066950 vs. TrEMBL
Match: A8QK92_VITVI (Germin-like protein 6 OS=Vitis vinifera GN=GER6 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.079e-14
Identity = 38/53 (71.70%), Postives = 43/53 (81.13%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NG G    AFVSFSSP PGLQILD+ALF +D PTEL+  TTF+DAA
Sbjct:  143 GLLHFQVNGAGIPSTAFVSFSSPQPGLQILDYALFANDLPTELVVQTTFLDAA 195

[Close]

BLAST of HO066950 vs. TrEMBL
Match: A5B155_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_017918 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.079e-14
Identity = 38/53 (71.70%), Postives = 43/53 (81.13%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+NG G    AFVSFSSP PGLQILD+ALF +D PTEL+  TTF+DAA
Sbjct:  128 GLLHFQVNGAGIPSTAFVSFSSPQPGLQILDYALFANDLPTELVVQTTFLDAA 180

[Close]

BLAST of HO066950 vs. TrEMBL
Match: C6TAV0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.715e-14
Identity = 41/53 (77.36%), Postives = 43/53 (81.13%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQIN G S+ LA VSFSS NPGLQI DFALFKS FPT LI  TTF+DAA
Sbjct:  143 GLLHFQINAGKSSALAIVSFSSSNPGLQIQDFALFKSGFPTPLIVQTTFLDAA 195

[Close]

BLAST of HO066950 vs. TAIR peptide
Match: AT1G72610.1 (| Symbols: GLP1, ATGER1, GER1 | germin-like protein 1 | chr1:27339302-27339928 REVERSE LENGTH=208)

HSP 1 Score: 74.3294 bits (181), Expect = 2.486e-14
Identity = 35/52 (67.31%), Postives = 41/52 (78.85%), Query Frame = -2

Query:  230 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDA 385
            GLLHFQIN G S+  A V+F+S NPGLQILDFALF +  PTEL+  TTF+DA
Sbjct:  143 GLLHFQINAGKSSASAVVTFNSANPGLQILDFALFANSLPTELVVGTTFLDA 194

[Close]

BLAST of HO066950 vs. TAIR peptide
Match: AT5G20630.1 (| Symbols: GLP3, GLP3A, GLP3B, ATGER3, GER3 | germin 3 | chr5:6975315-6975950 REVERSE LENGTH=211)

HSP 1 Score: 69.707 bits (169), Expect = 6.123e-13
Identity = 33/53 (62.26%), Postives = 40/53 (75.47%), Query Frame = -2

Query:  227 GLLHFQINGGGSNXLAFVSFSSPNPGLQILDFALFKSDFPTELITATTFIDAA 385
            GLLHFQ+N G    LAFV+F S +PGLQIL FALF +D P+EL+ ATTF+  A
Sbjct:  146 GLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSELVEATTFLSDA 198

The following BLAST results are available for this feature:

BLAST of HO066950 vs. SwissProt
BLAST of HO066950 vs. TrEMBL
BLAST of HO066950 vs. TAIR peptide

BLAST of HO066950 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
AB19A_PRUPE	6.413e-18	79.25	Auxin-binding protein ABP19a OS=Prunus persica GN=...	[more]
ABP20_PRUPE	1.747e-15	71.70	Auxin-binding protein ABP20 OS=Prunus persica GN=A...	[more]
AB19B_PRUPE	1.931e-14	69.23	Auxin-binding protein ABP19b OS=Prunus persica GN=...	[more]
GL31_ARATH	2.135e-13	67.31	Germin-like protein subfamily 3 member 1 OS=Arabid...	[more]
GL33_ARATH	5.259e-12	62.26	Germin-like protein subfamily 3 member 3 OS=Arabid...	[more]
GLP1_SINAL	8.970e-12	64.00	Germin-like protein 1 OS=Sinapis alba GN=GLP1 PE=2...	[more]
GLP1_BRANA	2.610e-11	62.26	Germin-like protein 1 OS=Brassica napus GN=GER1 PE...	[more]
GL814_ORYSJ	3.769e-10	52.83	Germin-like protein 8-14 OS=Oryza sativa subsp. ja...	[more]
GL813_ORYSJ	1.432e-9	52.83	Germin-like protein 8-13 OS=Oryza sativa subsp. ja...	[more]

BLAST of HO066950 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D4NXQ4_ARAHY	1.065e-18	86.79	Germin-like protein subfamily 3 member 4 OS=Arachi...	[more]
Q9AR81_PEA	1.817e-18	90.38	Germin-like protein OS=Pisum sativum GN=glp3 PE=2 ...	[more]
O49924_PEA	1.817e-18	90.38	Germin-like protein (Fragment) OS=Pisum sativum PE...	[more]
C7S8C4_SOYBN	2.009e-17	86.79	Germin-like protein 10 OS=Glycine max PE=2 SV=1	[more]
D1FMZ3_9ROSA	1.102e-15	77.36	Putative auxin-binding protein 2 OS=Prunus salicin...	[more]
C6K8P0_9ROSA	1.219e-14	73.58	Auxin-binding protein ABP19 (Fragment) OS=Prunus i...	[more]
B9RRB7_RICCO	1.219e-14	73.58	Auxin-binding protein ABP19a, putative OS=Ricinus ...	[more]
A8QK92_VITVI	2.079e-14	71.70	Germin-like protein 6 OS=Vitis vinifera GN=GER6 PE...	[more]
A5B155_VITVI	2.079e-14	71.70	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C6TAV0_SOYBN	2.715e-14	77.36	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of HO066950 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G72610.1	2.486e-14	67.31	\| Symbols: GLP1, ATGER1, GER1 \| germin-like protei...	[more]
AT5G20630.1	6.123e-13	62.26	\| Symbols: GLP3, GLP3A, GLP3B, ATGER3, GER3 \| germ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 9..45 score
None	No IPR available	SEG	seg	seg	coord: 40..59 score
None	No IPR available	SEG	seg	seg	coord: 18..37 score
None	No IPR available	SEG	seg	seg	coord: 63..73 score
IPR011051	Cupin, RmlC-type	SUPERFAMILY	51182	RmlC-like cupins	coord: 9..69 score: 6.6
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	no description	coord: 9..69 score: 2.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO066950 ID=HO066950; Name=HO066950; organism=Cicer arietinum; type=EST; length=413bp
AAAAAAAATAAAATATATTCCAATATGCAAGAAAATACCACACATACATT
TATTCATCTCANGNCACAAATTCCTAAAGTTATCTTCCTTAAAACTCAAA
ACTAACTTGACACAAAAACAAACAACACAATGACNCAAATCCAACAACAA
AAGAGAACACACTCTTACATGGACAATCATTAGTTTAACCACTACCTCCA
AGAACACCCTTAAGCTTCTTCACAACAGCAGCATCAATAAAAGTAGTAGC
AGTAATCAACTCAGTTGGAAAATCACTTTTAAACAAAGCAAAATCCAAAA
TCTGCAAACCAGGATTAGGACTACTAAAGCTCACAAAAGCAAGNGNATTA
GAACCNCCACCATTAATTTGAAAATGCAACAACCCCGCGTACCTGCCCGG
GNGGCCGCTCGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR011051	RmlC_Cupin_sf
IPR014710	RmlC-like_jellyroll

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