CK148699

EST Overview


Name	CK148699
Unique Name	CK148699
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	739

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of CK148699 vs. SwissProt
Match: PFPB_RICCO (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus communis GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 7.234e-72
Identity = 141/203 (69.46%), Postives = 153/203 (75.37%), Query Frame = -3

Query:   24 GWFGKKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 632
            G   KKKL  QS           +    L+  P    ++ KIETEK   +     + E++ ++  YK  FKGQSHFFGYEGRCGLPTNFD+ YCYALGY AGALL SGKTGLISSVGNL APV EWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWALKN YVSPGPIQF GPGSDA SHTLLLE G
Sbjct:  348 GGLWKKKLTSQSLQLFEFLPVAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQQGTYKAHFKGQSHFFGYEGRCGLPTNFDSTYCYALGYAAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYVSPGPIQFMGPGSDAASHTLLLELG 549

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BLAST of CK148699 vs. SwissProt
Match: PFPB_SOLTU (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum tuberosum PE=1 SV=2)

HSP 1 Score: 250.751 bits (639), Expect = 5.932e-66
Identity = 130/199 (65.33%), Postives = 148/199 (74.37%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL  Q            +    L+  P    ++ KIETEK   +     + + K K A Y  +FKGQ HFFGYEGRCGLP+NFD+ YCYALGYGAG+LLQSGKTGLISSVGNL APVEE TVGGTALT+LMDVERRHGKFKPVIKKAMVELEGAPFKKFAS R+EWAL N Y++PGPIQF GP ++ V+HTLLLE G
Sbjct:  352 KKKLTPQCLELFELLPLAIQEQLLLERDPHGNVQVAKIETEKMLIQMVETELDQRKQKGA-YNAQFKGQFHFFGYEGRCGLPSNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEELTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASKREEWALNNRYINPGPIQFVGPVANKVNHTLLLELG 549

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BLAST of CK148699 vs. SwissProt
Match: PFPA_SOLTU (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.393e-20
Identity = 50/151 (33.11%), Postives = 88/151 (58.28%), Query Frame = -3

Query:   30 EKTKKAKYKGE-FKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFK-------PVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLE 458
            ++ K+  YKG+ F    HFFGY+ R  LP+ FD +Y Y LG+    +L +G  G ++++ NL  P  +W  G + ++++M V +R+G+         P +  A V+L G  ++  +    ++ L + Y +PGP+QF GPG+DA + +L++E
Sbjct:  409 KRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHVCYHILAAGLNGYMATITNLKNPANKWHCGASPISAMMTV-KRYGRGPGKASIGVPALHPATVDLRGKSYELLSQNATKFLLDDVYRNPGPLQFDGPGADAKAGSLVVE 558

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BLAST of CK148699 vs. SwissProt
Match: PFPA_RICCO (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Ricinus communis GN=PFP-ALPHA PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.432e-19
Identity = 60/180 (33.33%), Postives = 95/180 (52.78%), Query Frame = -3

Query:   30 ETFRLPKIETEK------DAYRNG*N*VGEEKTKKAKYKGE-FKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVER------RHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLE 530
            ++ +L +IETEK      +A  N       ++ K+  YKG+ F    HFFGY+ R  LP+ FD +Y Y LG+    +L +G  G +++  NL  PV +W  G   + ++M V+R           KP I  A V+L+G  ++       ++ + + Y +PGP+QF GPG+DA   TL +E
Sbjct:  386 DSAQLSQIETEKLLAHLVEAEMN-------KRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHICYHVLAAGLNGYMATATNLKNPVNKWRCGAAPIAAMMTVKRWAQNPGATSIGKPAIHPAAVDLKGKAYELLRLNAVKFLMDDLYRNPGPLQFEGPGADAKPITLCVE 558

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BLAST of CK148699 vs. TrEMBL
Match: Q2HTG9_MEDTR (Pyrophosphate-dependent phosphofructokinase PfpB OS=Medicago truncatula GN=MtrDRAFT_AC150441g20v1 PE=4 SV=1)

HSP 1 Score: 305.834 bits (782), Expect = 2.355e-81
Identity = 155/204 (75.98%), Postives = 164/204 (80.39%), Query Frame = -3

Query:   21 GWFGKKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESGF 632
            G   KKKL DQS        +  +    L+  P    ++ KIETEK         +G+ K ++ KY GEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALL SGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCY SPGPIQFTGPGSDAVSHTLLLESGF
Sbjct:  361 GGLWKKKLTDQSLELFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIEMVETELGKRK-QEGKYNGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLHSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYTSPGPIQFTGPGSDAVSHTLLLESGF 563

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BLAST of CK148699 vs. TrEMBL
Match: B9HZE4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1093153 PE=4 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 3.083e-73
Identity = 150/221 (67.87%), Postives = 166/221 (75.11%), Query Frame = -3

Query:   24 QQLIAESMKFLS*-YCR*GWFGKKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 683
            QQLIAE  + L+      G   KKKL +QS           +    L+  P    ++ KIETEK   +     + E++ ++  YKG FKGQSHFFGYEGRCGLPTNFD+ YCYALGYGAGALL SGKTGLISSVGNL APV EWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKN Y+SPGPIQF GPGSDA+SHTLLLE G
Sbjct:  346 QQLIAELNEVLAHDVVDEGGQWKKKLTNQSLQLFDFLPPAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQEGSYKGHFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNRYISPGPIQFMGPGSDAISHTLLLELG 565

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BLAST of CK148699 vs. TrEMBL
Match: Q8W4M5_ARATH (Similar to pyrophosphate-dependent phosphofuctokinase beta subunit OS=Arabidopsis thaliana GN=At1g12000/ F12F1.13 PE=1 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 1.171e-72
Identity = 140/199 (70.35%), Postives = 157/199 (78.89%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS        +  +    L+  P    ++ KIETEK   +     + E++ +   YKG+F GQSHFFGYEGRCGLPTNFDA YCYALGYGAG LL SGKTGLISSVGNL APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWALKN Y+SPGPIQFTGPGSD++SHTLLLE G
Sbjct:  367 KKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYISPGPIQFTGPGSDSLSHTLLLELG 564

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BLAST of CK148699 vs. TrEMBL
Match: O65379_ARATH (F12F1.13 protein OS=Arabidopsis thaliana GN=F12F1.13 PE=4 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 1.171e-72
Identity = 140/199 (70.35%), Postives = 157/199 (78.89%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS        +  +    L+  P    ++ KIETEK   +     + E++ +   YKG+F GQSHFFGYEGRCGLPTNFDA YCYALGYGAG LL SGKTGLISSVGNL APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWALKN Y+SPGPIQFTGPGSD++SHTLLLE G
Sbjct:  375 KKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYISPGPIQFTGPGSDSLSHTLLLELG 572

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BLAST of CK148699 vs. TrEMBL
Match: D7KN79_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_888516 PE=4 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.998e-72
Identity = 140/199 (70.35%), Postives = 156/199 (78.39%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS        +  +    L+  P    ++ KIETEK   +     + E++ +   YKG+F GQSHFFGYEGRCGLPTNFDA YCYALGYGAG LL SGKTGLISSVGNL APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWAL N Y+SPGPIQFTGPGSDA+SHTLLLE G
Sbjct:  367 KKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALNNRYISPGPIQFTGPGSDALSHTLLLELG 564

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BLAST of CK148699 vs. TrEMBL
Match: B9RXE7_RICCO (Phosphofructokinase, putative OS=Ricinus communis GN=RCOM_0903240 PE=4 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 1.096e-70
Identity = 141/203 (69.46%), Postives = 153/203 (75.37%), Query Frame = -3

Query:   24 GWFGKKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 632
            G   KKKL  QS           +    L+  P    ++ KIETEK   +     + E++ ++  YK  FKGQSHFFGYEGRCGLPTNFD+ YCYALGY AGALL SGKTGLISSVGNL APV EWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWALKN YVSPGPIQF GPGSDA SHTLLLE G
Sbjct:  348 GGLWKKKLTSQSLQLFEFLPVAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQQGTYKAHFKGQSHFFGYEGRCGLPTNFDSTYCYALGYAAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYVSPGPIQFMGPGSDAASHTLLLELG 549

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BLAST of CK148699 vs. TrEMBL
Match: D7TED0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_59.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030491001 PE=4 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 1.432e-70
Identity = 137/199 (68.84%), Postives = 156/199 (78.39%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS        +  +    L+  P    ++ KIETEK   +     + E++ ++  Y G+F GQSHFFGYEGRCGLPTNFDANYCYALGYGAGALL SGKTGLISSVGNL APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKN YVSPGP+QF GP ++ ++HTL+LE G
Sbjct:  363 KKKLRNQSQQLFDFLPQAIQEQLLLERDPHGNVQVAKIETEKMLIQMVDTEL-EKRRQEHSYNGKFNGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLHSGKTGLISSVGNLGAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNRYVSPGPVQFVGPTANEINHTLMLELG 560

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BLAST of CK148699 vs. TrEMBL
Match: D7TR81_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_235.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00004820001 PE=4 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 4.606e-69
Identity = 136/200 (68.00%), Postives = 152/200 (76.00%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKA-KYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS           +    L+  P    ++ KIETEK   +     + + K++   KYKG FKGQSHFFGYEGRCG PTNFDA YCYALG+ AGALL  GKTGLISSVGNL APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVEL+GAPFKKFAS+RDEWALKN Y+SPGPIQF GP S+ V+HTLLLE G
Sbjct:  363 KKKLTNQSLQLFEFLPPAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGTYKYKGHFKGQSHFFGYEGRCGFPTNFDAAYCYALGFAAGALLHCGKTGLISSVGNLSAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASMRDEWALKNQYISPGPIQFNGPASNDVNHTLLLELG 562

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BLAST of CK148699 vs. TrEMBL
Match: B4FQM2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 4.606e-69
Identity = 133/199 (66.83%), Postives = 153/199 (76.88%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            K KL  +S        K  +    L+  P    ++ KIETEK         + E++  + +Y   F+GQ+HFFGYEGRCGLPTNFD+NYCYALGYGAGALLQSGKTGLISSVGNL APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVEL+ APFKK+ASLRDEWA+KN Y+SPGPIQF+GPGSD  +HTL+LE G
Sbjct:  364 KSKLQAESRELFEFLPKTIQEQLMLERDPHGNVQVAKIETEKMLISMVETEL-EKRKAEGRYSAHFRGQAHFFGYEGRCGLPTNFDSNYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDAAPFKKYASLRDEWAIKNRYISPGPIQFSGPGSDDSNHTLMLELG 561

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BLAST of CK148699 vs. TrEMBL
Match: O81437_ARATH (Putative phosphofructokinase beta subunit OS=Arabidopsis thaliana GN=AT4g04040 PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 7.857e-69
Identity = 135/199 (67.84%), Postives = 154/199 (77.39%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KK L  ++        +  +    L+  P    ++ KIETEK   +     + ++KT+   Y+ EF G+SHFFGYEGRCGLPTNFDA YCYALGYGAG+LLQSGKTGLISSVGNL APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFAS R+EWALKN Y+SPGPIQF GPGSDA +HTL+LE G
Sbjct:  383 KKNLKKETLEIFEFLPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTE-GTYEREFMGKSHFFGYEGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLMLELG 580

[Close]

BLAST of CK148699 vs. TAIR peptide
Match: AT1G12000.1 (| Symbols: | Phosphofructokinase family protein | chr1:4050159-4053727 REVERSE LENGTH=566)

HSP 1 Score: 276.944 bits (707), Expect = 7.700e-75
Identity = 140/199 (70.35%), Postives = 157/199 (78.89%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KKKL +QS        +  +    L+  P    ++ KIETEK   +     + E++ +   YKG+F GQSHFFGYEGRCGLPTNFDA YCYALGYGAG LL SGKTGLISSVGNL APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVELEGAPFKKFASLR+EWALKN Y+SPGPIQFTGPGSD++SHTLLLE G
Sbjct:  367 KKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETEL-EKRKQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYISPGPIQFTGPGSDSLSHTLLLELG 564

HSP 2 Score: 54.299 bits (129), Expect = 8.173e-8
Identity = 40/100 (40.00%), Postives = 53/100 (53.00%), Query Frame = -2

Query:  442 N*GVILFQR*--DFIPAAT--YCGINEIFVM-IL*MRVVWEKKTR*SVFGAF*IFPQAIQEQLMLERDPHGNVQVAQNRNRERCLSKWLKLSWGRENQEG 726
            N GVIL      DFIP        +NEI    ++    +W+KK        F + P+AIQEQLMLERDPHGNVQVA+    E+ L + ++    +  Q G
Sbjct:  327 NYGVILIPEGLIDFIPEVQELIAELNEILANEVVDENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIET-EKMLIQMVETELEKRKQAG 425

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BLAST of CK148699 vs. TAIR peptide
Match: AT4G04040.1 (| Symbols: MEE51 | Phosphofructokinase family protein | chr4:1939250-1942765 FORWARD LENGTH=569)

HSP 1 Score: 264.233 bits (674), Expect = 5.165e-71
Identity = 135/199 (67.84%), Postives = 154/199 (77.39%), Query Frame = -3

Query:   24 KKKLADQSXXXXXXXLKQFKNN*CLKEIPMETFRLPKIETEKDAYRNG*N*VGEEKTKKAKYKGEFKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLESG 620
            KK L  ++        +  +    L+  P    ++ KIETEK   +     + ++KT+   Y+ EF G+SHFFGYEGRCGLPTNFDA YCYALGYGAG+LLQSGKTGLISSVGNL APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFAS R+EWALKN Y+SPGPIQF GPGSDA +HTL+LE G
Sbjct:  369 KKNLKKETLEIFEFLPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTE-GTYEREFMGKSHFFGYEGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLMLELG 566

HSP 2 Score: 51.2174 bits (121), Expect = 6.919e-7
Identity = 27/61 (44.26%), Postives = 36/61 (59.02%), Query Frame = -2

Query:  442 WEKKTR*SVFGAF*IFPQAIQEQLMLERDPHGNVQVAQNRNRERCLSKWLKLSWGRENQEG 624
            W+K  +      F   PQ IQEQLMLERDPHGNVQVA+    E+ L + ++    ++  EG
Sbjct:  368 WKKNLKKETLEIFEFLPQTIQEQLMLERDPHGNVQVAKIET-EKMLIQMVETELEKKKTEG 427

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BLAST of CK148699 vs. TAIR peptide
Match: AT1G20950.1 (| Symbols: | Phosphofructokinase family protein | chr1:7297467-7301336 REVERSE LENGTH=614)

HSP 1 Score: 101.679 bits (252), Expect = 4.434e-22
Identity = 63/180 (35.00%), Postives = 102/180 (56.67%), Query Frame = -3

Query:   30 ETFRLPKIETEKD-AYRNG*N*VGEEKTKKAK---YKGE-FKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVER--RHGKF------KPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLE 530
            ++ +L +IETEK  AY      V  E  K+ K   YKG+ F    HFFGY+ R  LP+ FD +Y Y LG+    +L +G  G +++V NL +PV +W  G T +T++M V+   +   +      +P I  AMV+L+G  +        ++ +++ Y +PGP+Q+ GPG+DA + +L +E
Sbjct:  386 DSAQLSQIETEKLLAYL-----VETEMNKRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHICYHILAAGLNGYMATVTNLKSPVNKWKCGATPITAMMTVKHWSQDASYTLTSIGRPAIHPAMVDLKGKAYDLLRQNAQKFLMEDLYRNPGPLQYDGPGADAKAVSLCVE 560

[Close]

BLAST of CK148699 vs. TAIR peptide
Match: AT1G76550.1 (| Symbols: | Phosphofructokinase family protein | chr1:28722900-28726929 REVERSE LENGTH=617)

HSP 1 Score: 100.908 bits (250), Expect = 7.563e-22
Identity = 62/178 (34.83%), Postives = 100/178 (56.18%), Query Frame = -3

Query:   30 ETFRLPKIETEKD-AYRNG*N*VGEEKTKKAK---YKGE-FKGQSHFFGYEGRCGLPTNFDANYCYALGYGAGALLQSGKTGLISSVGNLCAPVEEWTVGGTALTSLMDVERRHGKF------KPVIKKAMVELEGAPFKKFASLRDEWALKNCYVSPGPIQFTGPGSDAVSHTLLLE 530
            ++ +L +IETEK  AY      V  E  K+ K   YKG+ F    HFFGY+ R  LP+ FD +Y Y LG+    +L +G  G +++V NL +PV +W  G   ++++M V+R           +PVI  A V+L+G  +        ++ +++ Y +PGP+Q+ GPG+DA + +L +E
Sbjct:  386 DSAQLSQIETEKLLAYL-----VETEMNKRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHVCYHILAAGLNGYMATVTNLKSPVNKWKCGAAPISAMMTVKRWSQNSGSTTIGRPVIHPASVDLKGKAYDLLRQNAQKFLMEDMYRNPGPVQYDGPGADAKAVSLCVE 558

The following BLAST results are available for this feature:

BLAST of CK148699 vs. SwissProt
BLAST of CK148699 vs. TrEMBL
BLAST of CK148699 vs. TAIR peptide

BLAST of CK148699 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
PFPB_RICCO	7.234e-72	69.46	Pyrophosphate--fructose 6-phosphate 1-phosphotrans...	[more]
PFPB_SOLTU	5.932e-66	65.33	Pyrophosphate--fructose 6-phosphate 1-phosphotrans...	[more]
PFPA_SOLTU	8.393e-20	33.11	Pyrophosphate--fructose 6-phosphate 1-phosphotrans...	[more]
PFPA_RICCO	1.432e-19	33.33	Pyrophosphate--fructose 6-phosphate 1-phosphotrans...	[more]

BLAST of CK148699 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2HTG9_MEDTR	2.355e-81	75.98	Pyrophosphate-dependent phosphofructokinase PfpB O...	[more]
B9HZE4_POPTR	3.083e-73	67.87	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8W4M5_ARATH	1.171e-72	70.35	Similar to pyrophosphate-dependent phosphofuctokin...	[more]
O65379_ARATH	1.171e-72	70.35	F12F1.13 protein OS=Arabidopsis thaliana GN=F12F1....	[more]
D7KN79_ARALY	1.998e-72	70.35	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9RXE7_RICCO	1.096e-70	69.46	Phosphofructokinase, putative OS=Ricinus communis ...	[more]
D7TED0_VITVI	1.432e-70	68.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TR81_VITVI	4.606e-69	68.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B4FQM2_MAIZE	4.606e-69	66.83	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
O81437_ARATH	7.857e-69	67.84	Putative phosphofructokinase beta subunit OS=Arabi...	[more]

BLAST of CK148699 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G12000.1	7.700e-75	70.35	\| Symbols: \| Phosphofructokinase family protein \|...	[more]
AT4G04040.1	5.165e-71	67.84	\| Symbols: MEE51 \| Phosphofructokinase family prot...	[more]
AT1G20950.1	4.434e-22	35.00	\| Symbols: \| Phosphofructokinase family protein \|...	[more]
AT1G76550.1	7.563e-22	34.83	\| Symbols: \| Phosphofructokinase family protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 10..25 score: NAcoord: 88..98 score
None	No IPR available	GENE3D	3.40.50.460	no description	coord: 2..86 score: 1.7
None	No IPR available	PANTHER	PTHR13697	PHOSPHOFRUCTOKINASE	coord: 14..149 score: 3.2
IPR000023	Phosphofructokinase domain	SUPERFAMILY	53784	Phosphofructokinase	coord: 3..149 score: 9.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148699 ID=CK148699; Name=CK148699; organism=Cicer arietinum; type=EST; length=739bp
ACCATTTATGCCTATGCTTAAAATCCAGACTCCAAAAGTAGAGTGTGACT
AACTGCATCAGATCCTGGGCCAGTGAATTGAATTGGACCTGGACTGACGT
AGCAATTCTTCAGGGCCCACTCGTCACGCAAGGAGGCAAATTTTTTGAAG
GGTGCCCCTTCAAGCTCCACCATTGCCTTCTTGATCACGGGCTTGAATTT
ACCGTGTCTCCTCTCCACATCCATCAGTGAAGTGAGTGCAGTTCCACCAA
CTGTCCACTCTTCAACTGGAGCACATAGGTTCCCAACCGATGATATTAGA
CCAGTCTTGCCGCTCTGAAGGAGGGCTCCAGCACCATAACCCAGAGCATA
GCAATAATTAGCATCAAAATTAGTTGGCAAACCACATCTCCCTTCATAAC
CGAAAAAGTGAGACTGCCCTTTAAATTCTCCTTTATATTTTGCCTTCTTG
GTTTTCTCTTCCCCAACTCAGTTTCAACCATTTCGATAAGCATCTTTCTC
TGTTTCTATTTTGGGCAACCTGAACGTTTCCATGGGGATCTCTTTCAAGC
ATCAATTGTTCTTGAATTGCTTGAGGAAAAATTCAAAAAGCTCCAAAGAC
TGATCAGCGAGTTTTTTTTTCCCAAACCACCCTCATCTACAATATCATGA
CAAAAATTTCATTGATTCCGCAATAAGTTGCTGCTGGAATGAAATCTTAC
CTTTGGAATAAAATGACCCCCTAGTTTAATTTCTTCGGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003872	6-phosphofructokinase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005945	6-phosphofructokinase complex

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000023	Phosphofructokinase_dom

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