FE671274

EST Overview
NameFE671274
Unique NameFE671274
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length521
Libraries
Library NameType
LIBEST_022820 Drought-stressed chickpea leave librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of FE671274 vs. SwissProt
Match: CAF1G_ARATH (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 63.929 bits (154), Expect = 5.129e-10
Identity = 29/41 (70.73%), Postives = 34/41 (82.93%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL+ E ALIRE+VD F F+AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDT 41
[Close]
BLAST of FE671274 vs. SwissProt
Match: CAF1F_ARATH (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana GN=CAF1-6 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 5.671e-9
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL EE  LIR++VD F ++AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDT 41
[Close]
BLAST of FE671274 vs. SwissProt
Match: CAF1J_ARATH (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.155e-8
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            M+ + K DS+ IREVW+ NL EEFALIREIVD+F++IAMDT
Sbjct:    1 MAETLKEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B9RNX3_RICCO (Ccr4-associated factor, putative OS=Ricinus communis GN=RCOM_0921770 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.422e-11
Identity = 32/41 (78.05%), Postives = 37/41 (90.24%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  KGDS+ IREVWNDNL+EEF LIREIVDQFN++AMDT
Sbjct:    1 MSIIPKGDSIHIREVWNDNLEEEFTLIREIVDQFNYVAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B9HAV1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_654220 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.137e-11
Identity = 34/43 (79.07%), Postives = 40/43 (93.02%), Query Frame = 1 
Query:  394 EMSVSS-KGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            EMS++  K +S+QIREVWNDNL+EEFALIREIVDQFNF+AMDT
Sbjct:    2 EMSIAPPKEESIQIREVWNDNLEEEFALIREIVDQFNFVAMDT 44
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B9SMT7_RICCO (Ccr4-associated factor, putative OS=Ricinus communis GN=RCOM_0470900 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 9.215e-11
Identity = 31/41 (75.61%), Postives = 39/41 (95.12%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  KGDS+QIREVWNDNL+EEF+LIREIVD++++IAMDT
Sbjct:    1 MSLLLKGDSIQIREVWNDNLEEEFSLIREIVDEYSYIAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B9GVJ6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_554871 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.053e-10
Identity = 32/41 (78.05%), Postives = 36/41 (87.80%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  KGDS+ IREVWNDNL+EEFALIREIVD F +IAMDT
Sbjct:    1 MSLLPKGDSIHIREVWNDNLEEEFALIREIVDDFPYIAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B7FJG2_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.681e-10
Identity = 29/36 (80.56%), Postives = 35/36 (97.22%), Query Frame = 1 
Query:  412 KGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            KGDS+QIREVWNDNL+EEF LIREIVD++N++AMDT
Sbjct:   10 KGDSIQIREVWNDNLEEEFVLIREIVDKYNYVAMDT 45
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B7FJ18_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.681e-10
Identity = 29/36 (80.56%), Postives = 35/36 (97.22%), Query Frame = 1 
Query:  412 KGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            KGDS+QIREVWNDNL+EEF LIREIVD++N++AMDT
Sbjct:   10 KGDSIQIREVWNDNLEEEFVLIREIVDKYNYVAMDT 45
[Close]
BLAST of FE671274 vs. TrEMBL
Match: B9IL41_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_809147 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.502e-10
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 1 
Query:  412 KGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            K DS+QIREVWNDNL+EEFALIREIVDQFN +AMDT
Sbjct:    7 KEDSIQIREVWNDNLEEEFALIREIVDQFNHVAMDT 42
[Close]
BLAST of FE671274 vs. TrEMBL
Match: D7U866_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_70.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034221001 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.574e-10
Identity = 32/41 (78.05%), Postives = 36/41 (87.80%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL+EEFALIR IVD+F FIAMDT
Sbjct:    1 MSLLPKSDSIQIREVWNDNLEEEFALIRGIVDEFPFIAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: A5C4B8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020892 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.574e-10
Identity = 32/41 (78.05%), Postives = 36/41 (87.80%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL+EEFALIR IVD+F FIAMDT
Sbjct:    1 MSLLPKSDSIQIREVWNDNLEEEFALIRGIVDEFPFIAMDT 41
[Close]
BLAST of FE671274 vs. TrEMBL
Match: C6TNY2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.738e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = 1 
Query:  403 VSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            V +K DS+QIREVWNDNL+EEFALIREIVD + +IAMDT
Sbjct:    4 VLAKSDSIQIREVWNDNLEEEFALIREIVDDYPYIAMDT 42
[Close]
BLAST of FE671274 vs. TAIR peptide
Match: AT2G32070.1 (| Symbols: | Polynucleotidyl transferase, ribonuclease H-like superfamily protein | chr2:13640829-13641656 REVERSE LENGTH=275)

HSP 1 Score: 63.929 bits (154), Expect = 5.665e-11
Identity = 29/41 (70.73%), Postives = 34/41 (82.93%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL+ E ALIRE+VD F F+AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDT 41
[Close]
BLAST of FE671274 vs. TAIR peptide
Match: AT1G80780.3 (| Symbols: | Polynucleotidyl transferase, ribonuclease H-like superfamily protein | chr1:30358517-30359583 FORWARD LENGTH=286)

HSP 1 Score: 60.4622 bits (145), Expect = 6.264e-10
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL EE  LIR++VD F ++AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDT 41
[Close]
BLAST of FE671274 vs. TAIR peptide
Match: AT1G80780.2 (| Symbols: | Polynucleotidyl transferase, ribonuclease H-like superfamily protein | chr1:30358517-30359341 FORWARD LENGTH=274)

HSP 1 Score: 60.4622 bits (145), Expect = 6.264e-10
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL EE  LIR++VD F ++AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDT 41
[Close]
BLAST of FE671274 vs. TAIR peptide
Match: AT1G80780.1 (| Symbols: | Polynucleotidyl transferase, ribonuclease H-like superfamily protein | chr1:30358517-30359341 FORWARD LENGTH=274)

HSP 1 Score: 60.4622 bits (145), Expect = 6.264e-10
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            MS+  K DS+QIREVWNDNL EE  LIR++VD F ++AMDT
Sbjct:    1 MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDT 41
[Close]
BLAST of FE671274 vs. TAIR peptide
Match: AT5G10960.1 (| Symbols: | Polynucleotidyl transferase, ribonuclease H-like superfamily protein | chr5:3464581-3465414 FORWARD LENGTH=277)

HSP 1 Score: 58.5362 bits (140), Expect = 2.380e-9
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1 
Query:  397 MSVSSKGDSVQIREVWNDNLDEEFALIREIVDQFNFIAMDT 519
            M+ + K DS+ IREVW+ NL EEFALIREIVD+F++IAMDT
Sbjct:    1 MAETLKEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDT 41
The following BLAST results are available for this feature:
BLAST of FE671274 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
CAF1G_ARATH5.129e-1070.73Probable CCR4-associated factor 1 homolog 7 OS=Ara... [more]
CAF1F_ARATH5.671e-965.85Probable CCR4-associated factor 1 homolog 6 OS=Ara... [more]
CAF1J_ARATH2.155e-868.29Probable CCR4-associated factor 1 homolog 10 OS=Ar... [more]
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BLAST of FE671274 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9RNX3_RICCO1.422e-1178.05Ccr4-associated factor, putative OS=Ricinus commun... [more]
B9HAV1_POPTR4.137e-1179.07Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SMT7_RICCO9.215e-1175.61Ccr4-associated factor, putative OS=Ricinus commun... [more]
B9GVJ6_POPTR2.053e-1078.05Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B7FJG2_MEDTR2.681e-1080.56Putative uncharacterized protein OS=Medicago trunc... [more]
B7FJ18_MEDTR2.681e-1080.56Putative uncharacterized protein OS=Medicago trunc... [more]
B9IL41_POPTR3.502e-1086.11Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7U866_VITVI4.574e-1078.05Whole genome shotgun sequence of line PN40024, sca... [more]
A5C4B8_VITVI4.574e-1078.05Putative uncharacterized protein OS=Vitis vinifera... [more]
C6TNY2_SOYBN1.738e-976.92Putative uncharacterized protein OS=Glycine max PE... [more]
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BLAST of FE671274 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5
Match NameE-valueIdentityDescription
AT2G32070.15.665e-1170.73| Symbols: | Polynucleotidyl transferase, ribonuc... [more]
AT1G80780.36.264e-1065.85| Symbols: | Polynucleotidyl transferase, ribonuc... [more]
AT1G80780.26.264e-1065.85| Symbols: | Polynucleotidyl transferase, ribonuc... [more]
AT1G80780.16.264e-1065.85| Symbols: | Polynucleotidyl transferase, ribonuc... [more]
AT5G10960.12.380e-968.29| Symbols: | Polynucleotidyl transferase, ribonuc... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 36..64
score: 3.8
IPR012337Ribonuclease H-likeSUPERFAMILY53098Ribonuclease H-likecoord: 23..64
score: 2.2
NoneNo IPR availableGENE3D3.30.420.10no descriptioncoord: 25..64
score: 1.6
NoneNo IPR availablePANTHERPTHR10797CCR4-ASSOCIATED FACTORcoord: 31..64
score: 4.3
NoneNo IPR availableSEGsegsegcoord: 20..34
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FE671274 ID=FE671274; Name=FE671274; organism=Cicer arietinum; type=EST; length=521bp
CAATTCAAGATCGCTGAAAGTCAGCTTTATTTTCTTCACTATAAGGTTCG
ATTATTCACTCCTTCAACTAGTACTTCACTTTCTAGGGTTTTTACTATAC
TTCAATAGTTCAACCCTAGTTCAGTTCAAAACGATTCGATTTACTCCTAA
CGCTTATTCTTTGGTTGATTTTGTTTCAGATCTAGTTGAATCTTAACCTC
AAACTTTCGATTCGAAGCTCAAATCACTGAAATCGAACCTTCTTCAGGTT
TTTACAACTGTACATGCTCGTTTTGTTGATTTTTTTTTGTAAGATTCGTT
TTAGATTTGTTAGGTTTTGAGATCTGAACGATTTTTGCGGATTTTGTTTA
CAGATCTGGTGGATTTGTTTTCGATTTTCATTTTGTCGTTCGTGAAATGT
CGGTTTCATCGAAAGGAGATTCGGTTCAAATTAGAGAGGTTTGGAACGAT
AATCTCGATGAAGAATTTGCATTGATCCGTGAAATCGTTGACCAATTCAA
CTTTATAGCTATGGATACTGA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
Vocabulary: INTERPRO
TermDefinition
IPR006941RNase_CAF1
IPR012337RNaseH-like_sf