HO062526

EST Overview


Name	HO062526
Unique Name	HO062526
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	711

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062526 vs. SwissProt
Match: TYRA1_ARATH (Arogenate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana GN=TYRAAT1 PE=1 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 1.015e-67
Identity = 136/221 (61.54%), Postives = 165/221 (74.66%), Query Frame = -1

Query:    7 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVISSRNQRSDDAMVS-NYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 666
            GR+L ML L STPINTKGYE+LL+L +N CGDSFDLYYGLF++N NSLE+LER+DLAFE LRK+L +RLH VVR Q FE   K   +  N         +N +++ +     RS+D +V   Y S  SG  +D ++LKI I+GFGNFGQFL KT+V+ GH VLAYSRSDY+  A +LGVSYF D DDL E+HPEVI+LCTSILSTEKVL+SLP QRLKRST
Sbjct:  240 GRLLGMLKLISTPINTKGYEALLDLAENICGDSFDLYYGLFVYNNNSLEVLERIDLAFEALRKELFSRLHGVVRKQSFEGEAKKVHVFPNCG-------ENDASLDM----MRSEDVVVKYEYNSQVSGSVNDGSRLKIGIVGFGNFGQFLGKTMVKQGHTVLAYSRSDYTDEAAKLGVSYFSDLDDLFEEHPEVIILCTSILSTEKVLESLPFQRLKRST 449

HSP 2 Score: 123.635 bits (309), Expect = 1.027e-27
Identity = 57/94 (60.64%), Postives = 76/94 (80.85%), Query Frame = -1

Query:    7 SGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 288
            +  S+ +  L+IAIIGFGN+GQFLA+T++  GH + A+SRSD+S  A+ LGVSYF D  DLCE+HP+V+LLCTSILS E +LK+LP QRL+R+T
Sbjct:   44 NSNSNATPPLRIAIIGFGNYGQFLAETLISQGHILFAHSRSDHSSAARRLGVSYFTDLHDLCERHPDVVLLCTSILSIENILKTLPFQRLRRNT 137

HSP 3 Score: 104.375 bits (259), Expect = 6.447e-22
Identity = 49/81 (60.49%), Postives = 64/81 (79.01%), Query Frame = -1

Query:  424 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESA 666
            GR+L+ L LESTPI+TKGYE+LL LV+NT GDSFDLYYGLF++N N++E LER  +AFE L+ QL  RLH    ++L +S+
Sbjct:  550 GRLLEKLSLESTPIDTKGYETLLKLVENTAGDSFDLYYGLFLYNPNAMEQLERFHVAFESLKTQLFGRLHSQHSHELAKSS 630

[Close]

BLAST of HO062526 vs. SwissProt
Match: TYRA2_ARATH (Arogenate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana GN=TYRAAT2 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.172e-31
Identity = 63/113 (55.75%), Postives = 85/113 (75.22%), Query Frame = -1

Query:    7 SSRNQRSDDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 345
            S R +  D A + +Y++    +   S+ LKIA++GFGNFGQFL+KT++RHGH ++ +SRSDYS  A  +G  +FD+  DLCEQHP+V+LLCTSILSTE VL+S P QRL+RST
Sbjct:   31 SLRIRAIDAAQIFDYETQLKSEYRKSSALKIAVLGFGNFGQFLSKTLIRHGHDLITHSRSDYSDAANSIGARFFDNPHDLCEQHPDVVLLCTSILSTESVLRSFPFQRLRRST 143

HSP 2 Score: 107.842 bits (268), Expect = 5.668e-23
Identity = 50/83 (60.24%), Postives = 66/83 (79.52%), Query Frame = -1

Query:  418 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVK 666
            GRVL+   +ES+PINTKGYE+LL+LV+NT  DSF+L+YGLFM+N N+LE LERLD+AFE ++K+L  RLH   R Q+F   V+
Sbjct:  244 GRVLEKYGVESSPINTKGYETLLDLVENTSSDSFELFYGLFMYNPNALEQLERLDMAFESVKKELFGRLHQQYRKQMFGGEVQ 326

HSP 3 Score: 20.7866 bits (42), Expect = 5.668e-23
Identity = 8/9 (88.89%), Postives = 8/9 (88.89%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD YAAGSQ
Sbjct:  229 HDYYAAGSQ 237

[Close]

BLAST of HO062526 vs. TrEMBL
Match: B9N704_POPTR (Arogenate dehydrogenase OS=Populus trichocarpa GN=AD2 PE=4 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.916e-77
Identity = 153/222 (68.92%), Postives = 179/222 (80.63%), Query Frame = -1

Query:    7 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVISSRN--QRSDDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 666
            GRVL+ML LESTPINTKGYESLL+LV+NT GDSFDLYYGLFM+N+N LEMLERLDLAFEDLRKQL  RLH+VVR QLF +A   + +++NY+    K   NG+A + SS    +    A    Y++ +S  SDDS+KLKIAI+GFGNFGQFLAKT V+ GH VLAYSR++YS  AQ LGVSYF +ADDLCE+HPEVI+LCTSILSTEKVLKSLP QRLKRST
Sbjct:  220 GRVLEMLKLESTPINTKGYESLLDLVENTSGDSFDLYYGLFMYNRNVLEMLERLDLAFEDLRKQLFGRLHEVVRKQLFGNAESRKVVQENYS----KGHLNGAAALSSSSKSVRFQGSAQTYKYEAENSSCSDDSSKLKIAIVGFGNFGQFLAKTFVQQGHSVLAYSRANYSDAAQRLGVSYFSNADDLCEEHPEVIVLCTSILSTEKVLKSLPFQRLKRST 437

HSP 2 Score: 138.658 bits (348), Expect = 4.623e-31
Identity = 71/118 (60.17%), Postives = 88/118 (74.58%), Query Frame = -1

Query:    7 SAIVISSRNQRSDDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 360
            +AI +S R Q S  +  +        Q+  S  LKIAIIGFGN+GQFLAKT++  GH VLA+SRSD+S  A+ LGVS+F D  DLCE+HP+VILLCTSI+STE VLKSLP+QRLKR+T
Sbjct:    2 TAITLSPRLQSSCSSSATKANPKHQTQNPSSYDLKIAIIGFGNYGQFLAKTLISQGHTVLAHSRSDHSLAAKSLGVSFFLDPHDLCERHPDVILLCTSIISTETVLKSLPLQRLKRNT 119

HSP 3 Score: 129.798 bits (325), Expect = 2.148e-28
Identity = 66/108 (61.11%), Postives = 83/108 (76.85%), Query Frame = -1

Query:  343 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVIS 666
            GRVL+ L +ESTP+NTKGYE+LLNLV+NT GDSFDLYYGLFM+N N++E LERLDLAFE L+ QL  RLH V+R QLF S+   Q L +  +    K++QN +A+V S
Sbjct:  538 GRVLEKLGMESTPVNTKGYETLLNLVENTAGDSFDLYYGLFMYNVNAMEQLERLDLAFESLKDQLFGRLHGVLRKQLFGSSDNSQDLSEEPS--DAKFSQNSAALVSS 643

[Close]

BLAST of HO062526 vs. TrEMBL
Match: B9SXN5_RICCO (Prephenate dehydrogenase, putative OS=Ricinus communis GN=RCOM_0003340 PE=4 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 9.838e-74
Identity = 155/239 (64.85%), Postives = 177/239 (74.06%), Query Frame = -1

Query:    7 HSHNTYC----------GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLR-DNYNYEVTKYAQNGSAIVISSRNQRSDDAMVS-NYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 687
            H H+ Y           GRVL+ML LESTPINTKGYESLL LV+NT  DSFDLYYGLFM+NKN+LEMLERLDLAFE LRKQL  RLHDVVR QLF +  + Q  + D+ N         G+A + +   +R   A     YK+  S   +D++KLKIAI+GFGNFGQFLAKT+VR GH VLAYSRSDYS  AQ+LGVSYF DA+DLCE+HPEVILLCTSILSTE VLKSLPVQRLKRST
Sbjct:  244 HEHDKYAAGSQFITHTVGRVLEMLSLESTPINTKGYESLLGLVENTAEDSFDLYYGLFMYNKNALEMLERLDLAFEALRKQLFGRLHDVVRKQLFGNEERGQFSQVDHANMHTY-----GAAFLSAPEAERFQGAAQPYEYKAKTSNCINDNSKLKIAIVGFGNFGQFLAKTLVRQGHTVLAYSRSDYSDEAQKLGVSYFSDANDLCEEHPEVILLCTSILSTENVLKSLPVQRLKRST 477

HSP 2 Score: 134.42 bits (337), Expect = 8.719e-30
Identity = 68/105 (64.76%), Postives = 84/105 (80.00%), Query Frame = -1

Query:  352 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAI 666
            GR+L+ L LESTPINTKGYE+LLNLV+NT GDSFDLYYGLFM+N N++E LERLDLAFE L+KQL  RLH V+R QLFE+  K Q LR+     V+K +Q+ +A+
Sbjct:  578 GRILEKLGLESTPINTKGYETLLNLVENTAGDSFDLYYGLFMYNVNAMEQLERLDLAFESLKKQLFGRLHGVLRKQLFENEEKSQVLRE--ESLVSKVSQDDAAL 680

HSP 3 Score: 131.724 bits (330), Expect = 5.651e-29
Identity = 65/89 (73.03%), Postives = 76/89 (85.39%), Query Frame = -1

Query:    7 DSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 273
            +S  LK+AIIGFGNFGQFLAKT+V  GH VLA+SR+D+S  A  LGVS+F D  DLCEQHP+VILLCTSI+STEKVLKSLP+QR KR+T
Sbjct:   72 NSHVLKVAIIGFGNFGQFLAKTLVAQGHTVLAHSRTDHSLEAHSLGVSFFLDPHDLCEQHPDVILLCTSIISTEKVLKSLPLQRFKRNT 160

[Close]

BLAST of HO062526 vs. TrEMBL
Match: D7MHN3_ARALY (Arogenate dehydrogenase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493579 PE=4 SV=1)

HSP 1 Score: 265.388 bits (677), Expect = 3.275e-69
Identity = 141/222 (63.51%), Postives = 165/222 (74.32%), Query Frame = -1

Query:    7 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFES-AVKVQTLRDNYNYEVTKYAQNGSAIVISSRNQRSDDAMVS-NYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 666
            GR+L ML L+STPINTKGYE+LL+L +NTCGDSFDLYYGLF++NKNSLE+LER+DLAFE LRK+L +RLH VVR Q FE  A KV    + Y  +             S    RS+D  V   Y    SG  +D ++LKI I+GFGNFGQFL KT+V+ GH VLAYSR+DY+ VA +LGVSYF D DDL E+HPEVILLCTSILSTEKVLKSLP QRLKRST
Sbjct:  218 GRLLGMLKLQSTPINTKGYEALLDLAENTCGDSFDLYYGLFVYNKNSLEVLERIDLAFEALRKELFSRLHGVVRKQSFEGEAKKVHVFPNGYENDA------------SLDMMRSEDVAVKYEYNPQVSGSVNDGSRLKIGIVGFGNFGQFLGKTMVKQGHTVLAYSRTDYTDVAAKLGVSYFSDLDDLFEEHPEVILLCTSILSTEKVLKSLPFQRLKRST 427

HSP 2 Score: 125.176 bits (313), Expect = 5.290e-27
Identity = 60/97 (61.86%), Postives = 76/97 (78.35%), Query Frame = -1

Query:    7 SNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 297
            +N    SD    L+IAIIGFGN+GQFLA+T++  GH + A+SRSD+S  A+ LGVSYF D  DLCE+HP+V+LLCTSILS E VLK+LP QRL+R+T
Sbjct:   19 NNPLPNSDPIPPLRIAIIGFGNYGQFLAETLISQGHILFAHSRSDHSSAARRLGVSYFADLHDLCERHPDVVLLCTSILSIENVLKTLPFQRLRRNT 115

HSP 3 Score: 106.686 bits (265), Expect = 1.947e-21
Identity = 50/81 (61.73%), Postives = 65/81 (80.25%), Query Frame = -1

Query:  424 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESA 666
            GR+L+ L LESTPI+TKGYE+LL LV+NT GDSFDLYYGLF++N N++E LER  +AFE L+KQL  RLH    ++L +S+
Sbjct:  528 GRLLEKLSLESTPIDTKGYETLLKLVENTAGDSFDLYYGLFLYNPNAMEQLERFHVAFESLKKQLFGRLHSQHSHELAKSS 608

[Close]

BLAST of HO062526 vs. TrEMBL
Match: A9RGN1_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_24068 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.877e-32
Identity = 70/106 (66.04%), Postives = 83/106 (78.30%), Query Frame = -1

Query:    7 DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 324
            D A   +Y+S    +  +  KLK+ I+GFGNFGQFLA  IV+ GH+VLAYSR+DYS  AQELGV+YF DADD CE+HPEV+LLCTSILSTE VL+SLP QRLKR T
Sbjct:    7 DAAQPFDYESVRLQELQNKNKLKVGIVGFGNFGQFLAARIVKQGHRVLAYSRTDYSEKAQELGVAYFRDADDFCEEHPEVVLLCTSILSTEAVLQSLPTQRLKRHT 112

HSP 2 Score: 112.079 bits (279), Expect = 1.649e-23
Identity = 52/78 (66.67%), Postives = 65/78 (83.33%), Query Frame = -1

Query:  433 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLF 666
            GRVL  L L+STPINTKGYE+LL LV+NT GDSF+LYYGLFM+N N+ E L+RL+LAF+  ++QL  +LHDV+R QLF
Sbjct:  212 GRVLGKLALQSTPINTKGYETLLGLVENTAGDSFELYYGLFMYNPNATEELDRLELAFDSTKRQLFGQLHDVLRKQLF 289

HSP 3 Score: 22.3274 bits (46), Expect = 1.649e-23
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD+YAAGSQ
Sbjct:  197 HDRYAAGSQ 205

[Close]

BLAST of HO062526 vs. TrEMBL
Match: D7TZ93_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017448001 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.757e-30
Identity = 68/106 (64.15%), Postives = 84/106 (79.25%), Query Frame = -1

Query:    7 DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 324
            D A   +Y++    Q + S  LKIAIIGFGNFGQFLAKT V+ GH VLA+SRS+Y  VA++LGVS+F D  DLCE+HPEV+LLC+SILST+ VLKSLP QRL+R+T
Sbjct:   82 DAAQPYDYETQLRTQFNKSNNLKIAIIGFGNFGQFLAKTFVKQGHTVLAHSRSNYVDVARKLGVSFFQDPHDLCEEHPEVVLLCSSILSTKSVLKSLPFQRLRRNT 187

HSP 2 Score: 59.3066 bits (142), Expect = 2.927e-8
Identity = 40/109 (36.70%), Postives = 54/109 (49.54%), Query Frame = -1

Query:  340 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVISS 666
            GRVL+   LEST INTKGYE+LLNL                         LERLD+AFE ++K++   +H + R QLFE    +   +D    +  K    GSA+ + S
Sbjct:  288 GRVLERFGLESTEINTKGYETLLNL-------------------------LERLDMAFESIKKEIFGYMHRLYRKQLFEDEGGLGVSKDKKVGQ--KLLHGGSALELPS 369

HSP 3 Score: 23.483 bits (49), Expect = 2.927e-8
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HDKYAAGSQ
Sbjct:  273 HDKYAAGSQ 281

[Close]

BLAST of HO062526 vs. TrEMBL
Match: A5BC69_VITVI (Putative uncharacterized protein (Fragment) OS=Vitis vinifera GN=VITISV_006626 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.757e-30
Identity = 68/106 (64.15%), Postives = 84/106 (79.25%), Query Frame = -1

Query:    7 DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 324
            D A   +Y++    Q + S  LKIAIIGFGNFGQFLAKT V+ GH VLA+SRS+Y  VA++LGVS+F D  DLCE+HPEV+LLC+SILST+ VLKSLP QRL+R+T
Sbjct:   53 DAAQPYDYETQLRTQFNKSNNLKIAIIGFGNFGQFLAKTFVKQGHTVLAHSRSNYVDVARKLGVSFFQDPHDLCEEHPEVVLLCSSILSTKSVLKSLPFQRLRRNT 158

HSP 2 Score: 112.849 bits (281), Expect = 4.393e-24
Identity = 57/109 (52.29%), Postives = 76/109 (69.72%), Query Frame = -1

Query:  340 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVISS 666
            GRVL+   LEST INTKGYE+LLNLV+NT GDSFDLYYGLF++N N++E LERLD+AFE ++K++   +H + R QLFE    +   +D    +  K    GSA+ + S
Sbjct:  259 GRVLERFGLESTEINTKGYETLLNLVENTAGDSFDLYYGLFVYNNNAMEQLERLDMAFESIKKEIFGYMHRLYRKQLFEDEGGLGVSKDKKVGQ--KLLHGGSALELPS 365

HSP 3 Score: 23.483 bits (49), Expect = 4.393e-24
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HDKYAAGSQ
Sbjct:  244 HDKYAAGSQ 252

[Close]

BLAST of HO062526 vs. TrEMBL
Match: A9S8A1_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_125750 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.294e-30
Identity = 65/106 (61.32%), Postives = 84/106 (79.25%), Query Frame = -1

Query:    7 DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 324
            D A   +Y+S    + + S+KLK+ I+GFGN+GQFLA  I   GH+VLA+SR+DYS  AQELGV+YF +ADD CE+HPEV+LLCTSILST  VL+SLP+QRL+R+T
Sbjct:   83 DAAQPFDYESRKLQELEKSSKLKVGIVGFGNYGQFLAARITSQGHRVLAHSRTDYSEKAQELGVTYFRNADDFCEEHPEVVLLCTSILSTVAVLQSLPLQRLRRNT 188

HSP 2 Score: 117.472 bits (293), Expect = 4.089e-25
Identity = 63/114 (55.26%), Postives = 82/114 (71.93%), Query Frame = -1

Query:  334 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAV--KVQTLRDNYNYEVTK-YAQNGSAIVISSRN 666
            GRVL  L LESTPINTKGYE+LL LV+NT GDSFDLYYGLFM+N N+ E LERL++AF+ +++QL  +LHDV+R QLF + V  K +   D  N   T   ++NG + + S+ N
Sbjct:  288 GRVLGKLGLESTPINTKGYETLLRLVENTEGDSFDLYYGLFMYNPNATEELERLEIAFDSIKRQLFGQLHDVLRKQLFGANVADKARLPHDPSNSSSTNGISKNGGSGIASNSN 401

HSP 3 Score: 22.3274 bits (46), Expect = 4.089e-25
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD+YAAGSQ
Sbjct:  273 HDRYAAGSQ 281

[Close]

BLAST of HO062526 vs. TrEMBL
Match: A9T2F3_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_15417 PE=4 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.914e-30
Identity = 66/106 (62.26%), Postives = 83/106 (78.30%), Query Frame = -1

Query:    7 DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 324
            D A   +++S  S + + ST LK+ I+GFGNFGQFLA+ IV+ GH VLA+SR DYS  A+ LGVS+F D DD CE+HPEV+LLCTSILSTE VL+SLP+QRL+R T
Sbjct:    7 DAAQPFDFESMRSQELEKSTPLKVGIVGFGNFGQFLAERIVKQGHTVLAHSRRDYSEKARALGVSFFRDPDDFCEEHPEVVLLCTSILSTEAVLQSLPLQRLRRHT 112

HSP 2 Score: 103.605 bits (257), Expect = 1.549e-20
Identity = 50/78 (64.10%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  433 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLF 666
            GRVL  L L+ST INTKGYE+LL LVQNT  DSF+LYYGLFM+N N+ E LERL++AF  +++QL  +LHD  R QLF
Sbjct:  212 GRVLGKLGLQSTSINTKGYETLLGLVQNTSNDSFELYYGLFMYNPNATEELERLEIAFHSVKRQLFGQLHDRFRKQLF 289

HSP 3 Score: 20.7866 bits (42), Expect = 1.549e-20
Identity = 7/9 (77.78%), Postives = 9/9 (100.00%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD++AAGSQ
Sbjct:  197 HDRFAAGSQ 205

[Close]

BLAST of HO062526 vs. TrEMBL
Match: A9NUN5_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 3.914e-30
Identity = 67/139 (48.20%), Postives = 96/139 (69.06%), Query Frame = -1

Query:    7 KVQTLRDNYNYEVTKYAQNGSAIVISSRNQRS-DDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 420
            ++   R  + + V K      + V+     ++ D A   +Y+   S   + ++ LKI I+GFGNFGQFLAKT+V+ GH VLA+SR+D+  VA+++GVS+F DADD CE+HPEVILLC+SI++TE VL+S P QRLKR+T
Sbjct:   35 RIWNSRSTFPFSVRKKQNRERSFVLRHLKIKALDAAQPFDYEFKKSEVLEKNSLLKIGIVGFGNFGQFLAKTLVKQGHTVLAHSRTDHGEVARKIGVSFFKDADDFCEEHPEVILLCSSIIATESVLRSFPTQRLKRNT 173

HSP 2 Score: 119.783 bits (299), Expect = 2.222e-25
Identity = 58/98 (59.18%), Postives = 74/98 (75.51%), Query Frame = -1

Query:  391 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLF------ESAVKVQTLRDNYN 666
            GR+L  L LESTPINTKGYE+LL +V NTCGDSFDLYYGLFM+N N+ E LERL++AF+ L+KQL+ +LH ++R QLF      ES+ ++  L D  N
Sbjct:  273 GRMLAKLNLESTPINTKGYETLLRIVDNTCGDSFDLYYGLFMYNNNATEQLERLEMAFDALKKQLMGQLHQILRKQLFEGSSSAESSQQISALTDTQN 370

[Close]

BLAST of HO062526 vs. TrEMBL
Match: D7KDI5_ARALY (Prephenate dehydrogenase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_888961 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 8.719e-30
Identity = 65/125 (52.00%), Postives = 89/125 (71.20%), Query Frame = -1

Query:    7 QNGSAIVISS----RNQRSDDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 369
            +N  A +IS     R +  D A + +Y++    +   S+ LKIA++GFGNFGQFL+KT++RHGH ++ +SRSDYS  A  +G  +F +  DLCEQHP+V+LLCTSILSTE VL+S P QRL+RST
Sbjct:   19 RNSPAFLISPPRSLRIRAIDAAQIFDYETQLKSEYRKSSALKIAVLGFGNFGQFLSKTLIRHGHDLITHSRSDYSDAANSIGARFFGNPHDLCEQHPDVVLLCTSILSTESVLRSFPFQRLRRST 143

HSP 2 Score: 107.842 bits (268), Expect = 8.528e-22
Identity = 50/83 (60.24%), Postives = 66/83 (79.52%), Query Frame = -1

Query:  418 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVK 666
            GRVL+   +ES+PINTKGYE+LL+LV+NT  DSF+L+YGLFM+N N+LE LERLD+AFE ++K+L  RLH   R Q+F   V+
Sbjct:  244 GRVLEKYGVESSPINTKGYETLLDLVENTSSDSFELFYGLFMYNPNALEQLERLDMAFESVKKELFGRLHQQYRKQMFGGEVQ 326

HSP 3 Score: 20.7866 bits (42), Expect = 8.528e-22
Identity = 8/9 (88.89%), Postives = 8/9 (88.89%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD YAAGSQ
Sbjct:  229 HDYYAAGSQ 237

[Close]

BLAST of HO062526 vs. TAIR peptide
Match: AT5G34930.1 (| Symbols: | arogenate dehydrogenase | chr5:13233391-13235522 FORWARD LENGTH=640)

HSP 1 Score: 256.529 bits (654), Expect = 1.029e-68
Identity = 136/221 (61.54%), Postives = 165/221 (74.66%), Query Frame = -1

Query:    7 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVKVQTLRDNYNYEVTKYAQNGSAIVISSRNQRSDDAMVS-NYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 666
            GR+L ML L STPINTKGYE+LL+L +N CGDSFDLYYGLF++N NSLE+LER+DLAFE LRK+L +RLH VVR Q FE   K   +  N         +N +++ +     RS+D +V   Y S  SG  +D ++LKI I+GFGNFGQFL KT+V+ GH VLAYSRSDY+  A +LGVSYF D DDL E+HPEVI+LCTSILSTEKVL+SLP QRLKRST
Sbjct:  240 GRLLGMLKLISTPINTKGYEALLDLAENICGDSFDLYYGLFVYNNNSLEVLERIDLAFEALRKELFSRLHGVVRKQSFEGEAKKVHVFPNCG-------ENDASLDM----MRSEDVVVKYEYNSQVSGSVNDGSRLKIGIVGFGNFGQFLGKTMVKQGHTVLAYSRSDYTDEAAKLGVSYFSDLDDLFEEHPEVIILCTSILSTEKVLESLPFQRLKRST 449

HSP 2 Score: 123.635 bits (309), Expect = 1.041e-28
Identity = 57/94 (60.64%), Postives = 76/94 (80.85%), Query Frame = -1

Query:    7 SGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 288
            +  S+ +  L+IAIIGFGN+GQFLA+T++  GH + A+SRSD+S  A+ LGVSYF D  DLCE+HP+V+LLCTSILS E +LK+LP QRL+R+T
Sbjct:   44 NSNSNATPPLRIAIIGFGNYGQFLAETLISQGHILFAHSRSDHSSAARRLGVSYFTDLHDLCERHPDVVLLCTSILSIENILKTLPFQRLRRNT 137

HSP 3 Score: 104.375 bits (259), Expect = 6.536e-23
Identity = 49/81 (60.49%), Postives = 64/81 (79.01%), Query Frame = -1

Query:  424 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESA 666
            GR+L+ L LESTPI+TKGYE+LL LV+NT GDSFDLYYGLF++N N++E LER  +AFE L+ QL  RLH    ++L +S+
Sbjct:  550 GRLLEKLSLESTPIDTKGYETLLKLVENTAGDSFDLYYGLFLYNPNAMEQLERFHVAFESLKTQLFGRLHSQHSHELAKSS 630

[Close]

BLAST of HO062526 vs. TAIR peptide
Match: AT1G15710.1 (| Symbols: | prephenate dehydrogenase family protein | chr1:5404505-5405581 FORWARD LENGTH=358)

HSP 1 Score: 136.732 bits (343), Expect = 1.188e-32
Identity = 63/113 (55.75%), Postives = 85/113 (75.22%), Query Frame = -1

Query:    7 SSRNQRSDDAMVSNYKSNDSGQSDDSTKLKIAIIGFGNFGQFLAKTIVRHGHKVLAYSRSDYSHVAQELGVSYFDDADDLCEQHPEVILLCTSILSTEKVLKSLPVQRLKRST 345
            S R +  D A + +Y++    +   S+ LKIA++GFGNFGQFL+KT++RHGH ++ +SRSDYS  A  +G  +FD+  DLCEQHP+V+LLCTSILSTE VL+S P QRL+RST
Sbjct:   31 SLRIRAIDAAQIFDYETQLKSEYRKSSALKIAVLGFGNFGQFLSKTLIRHGHDLITHSRSDYSDAANSIGARFFDNPHDLCEQHPDVVLLCTSILSTESVLRSFPFQRLRRST 143

HSP 2 Score: 107.842 bits (268), Expect = 5.730e-24
Identity = 50/83 (60.24%), Postives = 66/83 (79.52%), Query Frame = -1

Query:  418 GRVLDMLMLESTPINTKGYESLLNLVQNTCGDSFDLYYGLFMFNKNSLEMLERLDLAFEDLRKQLIARLHDVVRNQLFESAVK 666
            GRVL+   +ES+PINTKGYE+LL+LV+NT  DSF+L+YGLFM+N N+LE LERLD+AFE ++K+L  RLH   R Q+F   V+
Sbjct:  244 GRVLEKYGVESSPINTKGYETLLDLVENTSSDSFELFYGLFMYNPNALEQLERLDMAFESVKKELFGRLHQQYRKQMFGGEVQ 326

HSP 3 Score: 20.7866 bits (42), Expect = 5.730e-24
Identity = 8/9 (88.89%), Postives = 8/9 (88.89%), Query Frame = -3

Query:  683 HDKYAAGSQ 709
            HD YAAGSQ
Sbjct:  229 HDYYAAGSQ 237

The following BLAST results are available for this feature:

BLAST of HO062526 vs. SwissProt
BLAST of HO062526 vs. TrEMBL
BLAST of HO062526 vs. TAIR peptide

BLAST of HO062526 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
TYRA1_ARATH	1.015e-67	61.54	Arogenate dehydrogenase 1, chloroplastic OS=Arabid...	[more]
TYRA2_ARATH	1.172e-31	55.75	Arogenate dehydrogenase 2, chloroplastic OS=Arabid...	[more]

BLAST of HO062526 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9N704_POPTR	1.916e-77	68.92	Arogenate dehydrogenase OS=Populus trichocarpa GN=...	[more]
B9SXN5_RICCO	9.838e-74	64.85	Prephenate dehydrogenase, putative OS=Ricinus comm...	[more]
D7MHN3_ARALY	3.275e-69	63.51	Arogenate dehydrogenase OS=Arabidopsis lyrata subs...	[more]
A9RGN1_PHYPA	1.877e-32	66.04	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
D7TZ93_VITVI	1.757e-30	64.15	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BC69_VITVI	1.757e-30	64.15	Putative uncharacterized protein (Fragment) OS=Vit...	[more]
A9S8A1_PHYPA	2.294e-30	61.32	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9T2F3_PHYPA	3.914e-30	62.26	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
A9NUN5_PICSI	3.914e-30	48.20	Putative uncharacterized protein OS=Picea sitchens...	[more]
D7KDI5_ARALY	8.719e-30	52.00	Prephenate dehydrogenase family protein OS=Arabido...	[more]

BLAST of HO062526 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G34930.1	1.029e-68	61.54	\| Symbols: \| arogenate dehydrogenase \| chr5:13233...	[more]
AT1G15710.1	1.188e-32	55.75	\| Symbols: \| prephenate dehydrogenase family prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003099	Prephenate dehydrogenase	PROFILE	PS51176	PDH_ADH	coord: 151..237 score: 16
IPR004455	NADP oxidoreductase, coenzyme F420-dependent	PFAM	PF03807	F420_oxidored	coord: 152..232 score: 1.2
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 151..220 score: 7.2
None	No IPR available	PANTHER	PTHR14239	DUDULIN-RELATED	coord: 126..182 score: 4.5
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 151..235 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062526 ID=HO062526; Name=HO062526; organism=Cicer arietinum; type=EST; length=711bp
CGGGCAGGTACTTCTCTTCAACCTCTGCACAGGTAATGATTTAAGAACTT
TCTCTGTGGAAAGGATTGAAGTGCAAAGTAAAATCACTTCTGGATGCTGT
TCACAAAGATCATCTGCATCATCGAAATAGGAAACTCCCAATTCCTGAGC
CACATGAGAGTAGTCTGATCTAGAGTATGCTAACACTTTATGTCCATGAC
GTACAATAGTTTTTGCAAGGAATTGACCGAAGTTACCAAAACCAATTATT
GCGATCTTGAGCTTTGTGCTGTCATCAGACTGGCCAGAATCATTTGACTT
GTAATTACTTACCATAGCATCATCAGATCTCTGATTTCTGGAGGATATTA
CAATTGCGGATCCATTTTGGGCATACTTGGTTACTTCATAGTTGTAATTA
TCTCGTAAAGTTTGGACCTTTACAGCACTCTCAAACAACTGATTCCTCAC
AACATCATGCAAACGAGCAATGAGCTGTTTCCTCAAATCCTCAAAAGCCA
AATCCAATCTCTCCAACATCTCCAAAGAATTCTTATTAAACATAAACAGG
CCATAGTAAAGATCAAAACTATCCCCACAAGTATTTTGAACCAAATTCAA
CAAAGATTCATACCCTTTAGTATTAATAGGAGTGGACTCCAACATCAACA
TATCTAAAACCCTACCACAGTATGTGTTATGACTGTGACCCTGCTGCGTA
CTTATCATGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004665	prephenate dehydrogenase (NADP+) activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006571	tyrosine biosynthetic process
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR003099	Prephen_DH
IPR004455	NADP oxidoreductase, coenzyme F420-dependent
IPR016040	NAD(P)-bd_dom

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