HO062579

EST Overview


Name	HO062579
Unique Name	HO062579
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	468

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062579 vs. SwissProt
Match: RETO_PAPSO (Reticuline oxidase OS=Papaver somniferum GN=BBE1 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 6.321e-8
Identity = 23/38 (60.53%), Postives = 27/38 (71.05%), Query Frame = -1

Query:  298 ATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPL 411
            A +WG  YF  N+ RLVK KT +DP NVF H QSIPP+
Sbjct:  486 ARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 523

[Close]

BLAST of HO062579 vs. SwissProt
Match: RETOL_ARATH (Reticuline oxidase-like protein OS=Arabidopsis thaliana GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 6.321e-8
Identity = 27/42 (64.29%), Postives = 31/42 (73.81%), Query Frame = -1

Query:  298 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPL 423
            SY E   +G  YF +NF+RLVKIKT VDP N FR+EQSIP L
Sbjct:  495 SYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

[Close]

BLAST of HO062579 vs. SwissProt
Match: RETO_ESCCA (Reticuline oxidase OS=Eschscholzia californica GN=BBE1 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.137e-7
Identity = 21/36 (58.33%), Postives = 26/36 (72.22%), Query Frame = -1

Query:  298 SWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPL 405
            SWG  YF  N+ RL++ KT +DP NVF H QSIPP+
Sbjct:  484 SWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 519

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9TKQ6_RICCO (Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1964640 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.222e-15
Identity = 39/60 (65.00%), Postives = 49/60 (81.67%), Query Frame = -1

Query:  247 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIGS*KTRNPRTGNK 426
            TS+++A++WG+ YFKDNFNRLV++KTKVDP+N FRHEQSIPPLP S+   K R  R G K
Sbjct:  267 TSFIQASAWGSKYFKDNFNRLVQVKTKVDPDNFFRHEQSIPPLPASLRR-KRRGGRGGVK 325

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9SB04_RICCO (Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1335930 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.222e-15
Identity = 39/60 (65.00%), Postives = 49/60 (81.67%), Query Frame = -1

Query:  247 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIGS*KTRNPRTGNK 426
            TS+++A++WG+ YFKDNFNRLV++KTKVDP+N FRHEQSIPPLP S+   K R  R G K
Sbjct:  489 TSFIQASAWGSKYFKDNFNRLVQVKTKVDPDNFFRHEQSIPPLPASLRR-KRRGGRGGVK 547

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9SB02_RICCO (Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1335910 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.222e-15
Identity = 39/60 (65.00%), Postives = 49/60 (81.67%), Query Frame = -1

Query:  247 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIGS*KTRNPRTGNK 426
            TS+++A++WG+ YFKDNFNRLV++KTKVDP+N FRHEQSIPPLP S+   K R  R G K
Sbjct:  489 TSFIQASAWGSKYFKDNFNRLVQVKTKVDPDNFFRHEQSIPPLPASLRR-KRRGGRGGIK 547

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9GGJ1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_177387 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.037e-13
Identity = 32/44 (72.73%), Postives = 41/44 (93.18%), Query Frame = -1

Query:  295 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLP 426
            TS++EA++WG  YFKDNFNRL+++KTKVDP+N F+HEQSIPPLP
Sbjct:  464 TSFIEASAWGAKYFKDNFNRLIQVKTKVDPDNFFKHEQSIPPLP 507

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9I0Y5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1094922 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.768e-13
Identity = 34/48 (70.83%), Postives = 41/48 (85.42%), Query Frame = -1

Query:  289 RGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVS 432
            R TS+ EA+ WG  YFK+NFNRLV++KT VDP+N FRHEQSIPPLP+S
Sbjct:  485 RNTSFKEASVWGTKYFKNNFNRLVQVKTTVDPDNFFRHEQSIPPLPLS 532

[Close]

BLAST of HO062579 vs. TrEMBL
Match: D7MMH8_ARALY (FAD-binding domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_917168 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.776e-13
Identity = 33/43 (76.74%), Postives = 37/43 (86.05%), Query Frame = -1

Query:  295 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLP 423
            +++EA  WG  YFKDNFNRLVKIKTKVDP N FRHEQSIPP+P
Sbjct:  499 NFIEAKIWGPKYFKDNFNRLVKIKTKVDPNNFFRHEQSIPPMP 541

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9NE68_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_788911 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.497e-12
Identity = 33/48 (68.75%), Postives = 41/48 (85.42%), Query Frame = -1

Query:  283 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIG 426
            TS+++A+++G  YFKDNF RLV +KTKVDPEN FRHEQSIPPLP  +G
Sbjct:  485 TSFVDASAFGTNYFKDNFYRLVNVKTKVDPENFFRHEQSIPPLPQQMG 532

[Close]

BLAST of HO062579 vs. TrEMBL
Match: D7MMH7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_356638 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.955e-12
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = -1

Query:  292 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPV 423
            +++EA  WG  YFKDNFNRLV+IKTKVDP+N FRHEQSIP LPV
Sbjct:  309 NFIEAKIWGAKYFKDNFNRLVRIKTKVDPDNFFRHEQSIPTLPV 352

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9N5S7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_928811 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.955e-12
Identity = 33/48 (68.75%), Postives = 41/48 (85.42%), Query Frame = -1

Query:  283 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIG 426
            TS++EA+++G  YFKDNF RLV +KTKVDP+N FRHEQSIPPLP  +G
Sbjct:  486 TSFVEASAFGTNYFKDNFYRLVHVKTKVDPDNFFRHEQSIPPLPQQMG 533

[Close]

BLAST of HO062579 vs. TrEMBL
Match: B9N5S5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_928886 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.955e-12
Identity = 33/48 (68.75%), Postives = 41/48 (85.42%), Query Frame = -1

Query:  283 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIG 426
            TS++EA+++G  YFKDNF RLV +KTKVDP+N FRHEQSIPPLP  +G
Sbjct:  484 TSFVEASAFGTNYFKDNFYRLVHVKTKVDPDNFFRHEQSIPPLPQQMG 531

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT5G44380.1 (| Symbols: | FAD-binding Berberine family protein | chr5:17878873-17881369 REVERSE LENGTH=541)

HSP 1 Score: 73.9442 bits (180), Expect = 4.393e-14
Identity = 32/43 (74.42%), Postives = 37/43 (86.05%), Query Frame = -1

Query:  295 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLP 423
            +++EA  WG  YFK NF+RLVKIKTKVDPEN FRHEQSIPP+P
Sbjct:  498 NFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT5G44390.1 (| Symbols: | FAD-binding Berberine family protein | chr5:17882329-17884906 REVERSE LENGTH=542)

HSP 1 Score: 73.9442 bits (180), Expect = 4.393e-14
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = -1

Query:  292 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPV 426
            ++++EA  WG  YFKDNFNRLV+IK+KVDP+N FRHEQSIP LPV
Sbjct:  498 SNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLPV 542

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT5G44400.1 (| Symbols: | FAD-binding Berberine family protein | chr5:17886365-17888071 REVERSE LENGTH=537)

HSP 1 Score: 65.0846 bits (157), Expect = 2.041e-11
Identity = 29/43 (67.44%), Postives = 33/43 (76.74%), Query Frame = -1

Query:  298 TSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPL 426
            T   +A  WG  YFK+NFNRL+ IK KVDPEN FRHEQSIPP+
Sbjct:  494 TCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT1G30760.1 (| Symbols: | FAD-binding Berberine family protein | chr1:10918321-10920441 FORWARD LENGTH=534)

HSP 1 Score: 63.1586 bits (152), Expect = 7.755e-11
Identity = 29/46 (63.04%), Postives = 34/46 (73.91%), Query Frame = -1

Query:  304 VGGRGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIP 441
            + G+G+   +A  WGN YFK NF RLV+IK K DPEN FRHEQSIP
Sbjct:  488 MNGKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT1G01980.1 (| Symbols: | FAD-binding Berberine family protein | chr1:340374-341999 REVERSE LENGTH=541)

HSP 1 Score: 62.7734 bits (151), Expect = 1.013e-10
Identity = 30/48 (62.50%), Postives = 34/48 (70.83%), Query Frame = -1

Query:  280 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSIGS 423
            SY EA  +G  YF +NF+RLVK+KT VDPEN FR EQSIP LP    S
Sbjct:  493 SYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTKPSS 540

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT4G20860.1 (| Symbols: | FAD-binding Berberine family protein | chr4:11172726-11174318 FORWARD LENGTH=530)

HSP 1 Score: 60.4622 bits (145), Expect = 5.027e-10
Identity = 27/46 (58.70%), Postives = 32/46 (69.57%), Query Frame = -1

Query:  298 GRGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPL 435
            G  TS+ +A  WG  YFK NF RL  +K K+DP N FR+EQSIPPL
Sbjct:  484 GINTSFEDARKWGETYFKGNFKRLGLVKGKIDPTNFFRNEQSIPPL 529

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT1G11770.1 (| Symbols: | FAD-binding Berberine family protein | chr1:3975679-3977289 FORWARD LENGTH=536)

HSP 1 Score: 60.4622 bits (145), Expect = 5.027e-10
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = -1

Query:  295 SYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLP 423
            SY  A  +G MYF +NF+RLVK+KT VDP+N FR EQSIP LP
Sbjct:  488 SYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT4G20820.1 (| Symbols: | FAD-binding Berberine family protein | chr4:11150160-11151758 FORWARD LENGTH=532)

HSP 1 Score: 59.6918 bits (143), Expect = 8.574e-10
Identity = 27/47 (57.45%), Postives = 33/47 (70.21%), Query Frame = -1

Query:  301 VGGRGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPP 441
            VG + + Y E  SWG  YFK+NF RLV++KT VDP + F  EQSIPP
Sbjct:  479 VGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT1G30700.1 (| Symbols: | FAD-binding Berberine family protein | chr1:10892623-10894437 FORWARD LENGTH=527)

HSP 1 Score: 59.3066 bits (142), Expect = 1.120e-9
Identity = 28/47 (59.57%), Postives = 34/47 (72.34%), Query Frame = -1

Query:  295 GRGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLP 435
            G+ +SY+E   +G  YF  NF RLVKIKT+VD  N FR+EQSIP LP
Sbjct:  481 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

[Close]

BLAST of HO062579 vs. TAIR peptide
Match: AT5G44440.1 (| Symbols: | FAD-binding Berberine family protein | chr5:17910694-17912295 REVERSE LENGTH=533)

HSP 1 Score: 58.151 bits (139), Expect = 2.495e-9
Identity = 26/46 (56.52%), Postives = 33/46 (71.74%), Query Frame = -1

Query:  304 VGGRGTSYLEATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIP 441
            +G + T Y E  SWG  YFK+NF RLV++KT+VDP + F  EQSIP
Sbjct:  482 LGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

The following BLAST results are available for this feature:

BLAST of HO062579 vs. SwissProt
BLAST of HO062579 vs. TrEMBL
BLAST of HO062579 vs. TAIR peptide

BLAST of HO062579 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
RETO_PAPSO	6.321e-8	60.53	Reticuline oxidase OS=Papaver somniferum GN=BBE1 P...	[more]
RETOL_ARATH	6.321e-8	64.29	Reticuline oxidase-like protein OS=Arabidopsis tha...	[more]
RETO_ESCCA	3.137e-7	58.33	Reticuline oxidase OS=Eschscholzia californica GN=...	[more]

BLAST of HO062579 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9TKQ6_RICCO	3.222e-15	65.00	Reticuline oxidase, putative OS=Ricinus communis G...	[more]
B9SB04_RICCO	3.222e-15	65.00	Reticuline oxidase, putative OS=Ricinus communis G...	[more]
B9SB02_RICCO	3.222e-15	65.00	Reticuline oxidase, putative OS=Ricinus communis G...	[more]
B9GGJ1_POPTR	1.037e-13	72.73	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9I0Y5_POPTR	1.768e-13	70.83	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MMH8_ARALY	8.776e-13	76.74	FAD-binding domain-containing protein OS=Arabidops...	[more]
B9NE68_POPTR	1.497e-12	68.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MMH7_ARALY	1.955e-12	75.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9N5S7_POPTR	1.955e-12	68.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N5S5_POPTR	1.955e-12	68.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of HO062579 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G44380.1	4.393e-14	74.42	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT5G44390.1	4.393e-14	71.11	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT5G44400.1	2.041e-11	67.44	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT1G30760.1	7.755e-11	63.04	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT1G01980.1	1.013e-10	62.50	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT4G20860.1	5.027e-10	58.70	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT1G11770.1	5.027e-10	65.12	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT4G20820.1	8.574e-10	57.45	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT1G30700.1	1.120e-9	59.57	\| Symbols: \| FAD-binding Berberine family protein...	[more]
AT5G44440.1	2.495e-9	56.52	\| Symbols: \| FAD-binding Berberine family protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 20..55 score: 2.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062579 ID=HO062579; Name=HO062579; organism=Cicer arietinum; type=EST; length=468bp
GCACTTAAAATAGTGTTCTCTATTAAATATTTGTAATTTATGCAATCTAA
AATCTTTCCACAAACATAATAACAAAACAAAAAAAGCGATGTTTAAGCAC
TGCATCGGTATCTGACAAATTACAACATTAAAAACACAAAAAACAGAAAA
CTTCAAAATATATCCTTTATTTTGTGTACTTCACCCAAACAACCCTTGCA
TCAAATTTATAGTCACCAACTTAGAGAAACTCTGGCAAATCAGTGCCTTA
TTCCCAGTTCTTGGATTTCTTGTTTTTTAACTGCCTATTGAAACTGGAAG
TGGTGGAATACTCTGCTCATGCCTGAACACATTTTCAGGATCCACTTTAG
TCTTAATTTTCACCAACCTGTTGAAGTTATCTTTGAAATACATGTTACCC
CAAGAAGTTGCTTCTAAATAGCTTGTACCTCGGCCCCCCACCACCCTAAT
TGAATTCCCGCGGCCGCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding

Vocabulary: **INTERPRO**
Term	Definition
IPR012951	BBE

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